Compact cell culture device

Abstract

The utility model belongs to the technical field of cell culture, and particularly relates to a compact cell culture device which comprises a base, a mounting groove is formed in the base, limiting grooves are symmetrically formed in the inner wall of the base, threaded holes are formed in the two limiting grooves, a culture dish is arranged in the mounting groove, and the culture dish is arranged in the mounting groove. Positioning grooves are symmetrically formed in the inner wall of the mounting groove; the culture dish is placed in the mounting groove, the reset spring is extruded, the rubber clamping block is located on the outer side of the positioning groove, the positioning spring pushes the rubber clamping block into the positioning groove, the culture dish can be mounted and fixed through the fixing bolt, the fixing bolt is screwed out, the culture dish is pressed downwards, and the elastic force of the reset spring is larger than that of the positioning spring. And therefore, the pressure borne by the reset spring is reduced in a short time, the culture dish can be pushed upwards, the culture dish can be taken out, and the effect of being convenient to mount and dismount is achieved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to cell culture technical field, specifically a compact cell culture device. BACKGROUND

[0002] Cell model is one of the most widely used models in space life science application, and cell culture is the basis of cell experiment, but it is limited by the difference between experimental environment and ideal cell culture environment, for example, lower carbon dioxide level under experimental condition will lead to pH value rising, temperature change will affect cell metabolism, etc., in order to eliminate these adverse effects, the environmental conditions during experiment must be controlled, and the living environment of cell in vivo is simulated as far as possible, so a compact cell culture device is needed.

[0003] A compact online observation or independent live cell culture device is disclosed in one of the Chinese patents with the authorized announcement number CN219930129U, which comprises a temperature control unit, a cell culture unit and a fluid control unit, the utility model has the advantages of compact structure, small volume, good compatibility and portability, the device can provide basic physiological conditions for cell culture, allows quick disassembly and installation to replace samples, and has good stability.The live cell culture device combines a microscopic imaging system, integrates cell culture and observation, can realize continuous observation of cells, and can record images of cells under fluorescence / microscope in real time.

[0004] However, the above scheme still has some problems, the culture dish is located in the device, and no disassembly component is arranged, thereby causing the culture dish to be inconvenient to assemble and disassemble, therefore, the compact cell culture device is proposed for the above problems. SUMMARY

[0005] In order to make up for the deficiency of the prior art, in view of the problems existing in the prior art, the utility model provides a compact cell culture device.

[0006] The utility model discloses a compact cell culture device that solves the technical problems thereof, which comprises: a base, an installation groove is formed in the inside of the base, limit grooves are symmetrically formed in the inner wall of the base, threaded holes are formed in the limit grooves, a culture dish is arranged in the installation groove, positioning grooves are symmetrically formed in the inner wall of the installation groove, limit rods are fixedly connected to the two sides of the culture dish in a symmetrical manner, the limit rods are movably connected to the inside of the limit grooves, fixing bolts are threadedly connected to the limit rods, the fixing bolts are threadedly connected to the inside of the threaded holes, rubber clamping blocks are fixedly connected to the two sides of the culture dish, positioning springs are fixedly connected to the inside of the rubber clamping blocks, one end of the positioning springs is fixedly connected to the culture dish, reset springs are fixedly connected to the four corners of the bottom of the culture dish, the elastic force of the reset springs is greater than that of the positioning springs, so that the pressure received by the reset springs changes from large to small in a short time, the culture dish can be pushed upward, and the culture dish can be taken out.

[0007] Preferably, one end of the reset spring is in close contact with the inside of the installation groove, the rubber clamping block is movably connected to the inside of the positioning groove, a sliding groove is symmetrically formed in the base, and a ventilation groove is symmetrically formed in the inner wall of the installation groove. Under the action of the elastic force of the positioning spring, the rubber clamping block is pushed into the positioning groove, so that the position of the culture dish is preliminarily fixed.

[0008] Preferably, a baffle is slidably connected to the inside of the sliding groove, and damping strips are fixedly connected to the baffle in a symmetrical manner. The damping strips are attached to the inner wall of the installation groove, the damping strips can increase the friction with the inner wall of the base, and the sealing performance of the culture dish is improved.

[0009] Preferably, a limiting block is slidably connected to the inside of the sliding groove, and the limiting block extends to the upper side of the base. A fixing groove is symmetrically formed in the inside of the base, the movement range of the limiting block can be limited by the sliding groove, and the situation that the limiting block falls off is avoided.

[0010] Preferably, a grid plate is fixedly connected to the inside of the base, the grid plate is located below the culture dish, and the reset spring passes through the grid plate. A cooling fan is rotatably connected to the bottom of the grid plate, the grid plate is located below the culture dish, and the situation that the culture dish is pressed too much and the cooling fan is damaged is avoided.

[0011] Preferably, a driving assembly is fixedly connected to one end of the cooling fan, the driving assembly is fixedly connected to the bottom of the base, and a heating plate is arranged in the fixing groove. When the driving assembly is started, the cooling fan is driven to rotate, air can be blown into the ventilation groove from below the grid plate, and then the air is discharged outward from the ventilation groove, so that the temperature is reduced.

[0012] The utility model discloses the beneficial effect lies in: the petri dish is put into the installation groove, and the reset spring is extruded, and the rubber clamping block is located the outside of the positioning groove, and the positioning spring pushes the rubber clamping block into the positioning groove, and can install and fix the petri dish with fixed bolt, and the fixed bolt is screwed out, and the petri dish is pressed downward, and the elastic force of reset spring is greater than the elastic force of positioning spring, so that reset spring is pressed from big to small in a short time, can push the petri dish upward, can take out the petri dish, and further has the effect that convenient to assemble and disassemble.

[0013] The utility model discloses the temperature required when culturing cell is different, thereby when culturing cell needs high temperature, start heating plate to heat treatment is carried out to the inside of base, if temperature is too high, start drive assembly, drive cooling fan rotation, can blow into ventilation groove from the below of grid plate, then export from ventilation groove, play the role of reducing temperature, further make the device can satisfy the demand under different situations of culturing cell. BRIEF DESCRIPTION OF DRAWINGS

[0014] In order to more clearly illustrate the technical scheme in the embodiment of the utility model or prior art, the following will be to the embodiment or prior art description needed to use the drawing briefly introduced, obviously, the following description in the drawing is only some embodiments of the utility model, for the ordinary skilled person in the art comes, under the premise of not paying the creative labor, can also obtain other drawings according to these drawings.

[0015] Figure 1 It is whole structure schematic diagram;

[0016] Figure 2 It is base schematic diagram;

[0017] Figure 3 It is whole cross section schematic diagram;

[0018] Figure 4 It is petri dish schematic diagram;

[0019] Figure 5 It is temperature regulating subassembly cross section schematic diagram.

[0020] In the drawing: 1, base;2, installation groove;3, limit slot;4, threaded hole;5, petri dish;6, positioning groove;7, limit rod;8, fixed bolt;9, rubber clamping block;10, reset spring;11, sliding slot;12, ventilation groove;13, baffle;14, damping strip;15, limit block;16, fixed slot;17, grid plate;18, cooling fan;19, drive assembly;20, heating plate. DETAILED DESCRIPTION

[0021] Clearly and completely describe the technical scheme in the embodiments of the utility model with reference to the drawings in the embodiments of the utility model, obviously, the described embodiments are only a part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the ordinary skill in the art without creative labor belong to the range of the utility model protection.

[0022] Please refer to Figures 1-5 As shown in FIG. 1, a compact cell culture device comprises: a base 1, an installation groove 2 is opened in the inside of the base 1, limit grooves 3 are symmetrically opened in the inner wall of the base 1, threaded holes 4 are opened on the two sets of limit grooves 3, a culture dish 5 is arranged in the inside of the installation groove 2, positioning grooves 6 are symmetrically opened in the inner wall of the installation groove 2, limit rods 7 are fixedly connected on the two sides of the culture dish 5 symmetrically, the limit rods 7 are movably connected in the inside of the limit grooves 3, fixed bolts 8 are threadedly connected on the limit rods 7, the fixed bolts 8 are threadedly connected in the inside of the threaded holes 4, rubber clamping blocks 9 are fixedly connected on the two sides of the culture dish 5, positioning springs are fixedly connected in the inside of the rubber clamping blocks 9, one end of the positioning springs is fixedly connected on the culture dish 5, reset springs 10 are fixedly connected on the four corners of the bottom of the culture dish 5, one end of the reset springs 10 is tightly attached in the inside of the installation groove 2, the rubber clamping blocks 9 are movably connected in the inside of the positioning grooves 6, slide grooves 11 are symmetrically opened on the base 1, ventilation grooves 12 are symmetrically opened in the inner wall of the installation groove 2, a baffle 13 is slidably connected in the inside of the slide groove 11, damping strips 14 are fixedly connected on the baffle 13 symmetrically, the damping strips 14 are attached to the inner wall of the installation groove 2, limit blocks 15 are slidably connected in the inside of the slide groove 11, and the limit blocks 15 extend to the upper side of the base 1, fixed grooves 16 are symmetrically opened in the inside of the base 1;

[0023] In order to eliminate the adverse effects during cell culture, it is necessary to control the environmental conditions during the experiment, and to simulate the living environment of cells in vivo as much as possible, and thus a compact cell culture device is needed. In actual use, the culture dish 5 is placed in the installation groove 2, so that the limit rod 7 is located in the inside of the limit groove 3, and the reset spring 10 is extruded at the same time. When the threaded hole on the limit rod 7 is opposite to the threaded hole 4 on the base 1, the rubber clamping block 9 is located outside the positioning groove 6 and is pushed into the positioning groove 6 by the elastic force of the positioning spring itself, thereby preliminarily fixing the position of the culture dish 5. Then, the position of the limit rod 7 is fixed by using the fixed bolt 8, so that the culture dish 5 can be installed by cooperation. When the culture dish 5 needs to be taken out, the fixed bolt 8 is unscrewed, and the culture dish 5 is pressed downward. Because the elastic force of the reset spring 10 is greater than that of the positioning spring, the reset spring 10 is subjected to a pressure that changes from large to small in a short time, so that the culture dish 5 is pushed upward, the rubber clamping block 9 is moved out of the positioning groove 6, and the culture dish 5 can be taken out, thereby achieving the effect of convenient installation and removal.

[0024] When the cell culture, the culture dish 5 is fixed in the installation groove 2, the two sides of the limiting block 15 along the sliding groove 11 to the middle, so that the two groups of baffle 13 paste, so as to close the culture dish 5 above, while the damping strip 14 can increase the friction with the inner wall of the base 1, thereby improving the sealing of the culture dish 5, the two groups of limiting block 15 to the two sides of the pull, that is, the two groups of baffle 13 can be separated, so that the air into the culture dish 5, so as to meet the needs of different situations of cell culture.

[0025] Please refer to Figures 1-5 As shown in the figure, the base 1 is fixedly connected with the grid plate 17, the grid plate 17 is located below the culture dish 5, and the reset spring 10 passes through the grid plate 17, the bottom of the grid plate 17 is rotatably connected with the cooling fan 18, one end of the cooling fan 18 is fixedly connected with the driving assembly 19, the driving assembly 19 is fixedly connected with the bottom of the base 1, and the inside of the fixed groove 16 is provided with a heating plate 20;

[0026] The grid plate 17 is located below the culture dish 5, so as to avoid the culture dish 5 to press too much and cause the cooling fan 18 to be damaged, when the cell culture needs high temperature, the heating plate 20 is started, so as to heat the inside of the base 1, if the temperature is too high, the driving assembly 19 is started, driving the cooling fan 18 to rotate, so as to blow the wind from below the grid plate 17 into the ventilation groove 12, and then discharge from the ventilation groove 12, thereby playing a role in reducing the temperature.

[0027] The working principle, the culture dish 5 is put into the installation groove 2, so that the limiting rod 7 is located in the limiting groove 3, at the same time, the reset spring 10 is extruded, when the threaded hole on the limiting rod 7 is opposite to the threaded hole 4 on the base 1, the rubber clamping block 9 is located outside the positioning groove 6, the positioning spring pushes the rubber clamping block 9 into the positioning groove 6, then the position of the limiting rod 7 is fixed by using the fixed bolt 8, so that the two can be installed, the culture dish 5 is started, the heating plate 20 is started, so as to heat the inside of the base 1, if the temperature is too high, the driving assembly 19 is started, driving the cooling fan 18 to rotate, so as to blow the wind from below the grid plate 17 into the ventilation groove 12, and then discharge from the ventilation groove 12, thereby playing a role in reducing the temperature, so that the temperature can meet the needs of cell culture, when the cell culture, the two sides of the limiting block 15 along the sliding groove 11 to the middle, so that the two groups of baffle 13 paste, so as to close the culture dish 5 above, the two groups of limiting block 15 to the two sides of the pull, that is, the two groups of baffle 13 can be separated, so that the air into the culture dish 5, so as to meet the needs of different situations of cell culture, after the cell culture, the culture dish 5 is pressed downward, because the elastic force of the reset spring 10 is greater than that of the positioning spring, so that the reset spring 10 is subjected to pressure from large to small in a short time, the culture dish 5 can be pushed upward, so that the rubber clamping block 9 moves out of the positioning groove 6, that is, the culture dish 5 can be taken out.

[0028] In the description of the present specification, the description referring to the terms "one embodiment", "an example", "a specific example" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Also, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0029] The basic principle, main features and advantages of the present application have been shown and described above. It should be understood by those skilled in the art that the present application is not limited by the above embodiments, and the above embodiments and descriptions in the specification are only to illustrate the principle of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application.

Claims

1. A compact cell culture device, characterized in that: include: The base (1) has an installation groove (2) inside. The inner wall of the base (1) has symmetrically opened limit grooves (3). Both sets of limit grooves (3) have threaded holes (4). The installation groove (2) has a petri dish (5) inside. The inner wall of the installation groove (2) has symmetrically opened positioning grooves (6). The two sides of the petri dish (5) are symmetrically fixedly connected to limit rods (7). The limit rods (7) are movably connected inside the limit grooves (3). The limit rods (7) are threadedly connected to fixing bolts (8). The fixing bolts (8) are threadedly connected inside the threaded holes (4). Both sides of the petri dish (5) are fixedly connected to rubber blocks (9). The rubber blocks (9) are fixedly connected to positioning springs. One end of the positioning spring is fixedly connected to the petri dish (5). The four corners of the bottom of the petri dish (5) are fixedly connected to reset springs (10).

2. The compact cell culture device according to claim 1, characterized in that: One end of the reset spring (10) is tightly attached to the inside of the mounting groove (2), the rubber block (9) is movably connected to the inside of the positioning groove (6), the base (1) is symmetrically provided with sliding grooves (11), and the inner wall of the mounting groove (2) is symmetrically provided with ventilation grooves (12).

3. A compact cell culture device according to claim 2, characterized in that: The slide groove (11) is slidably connected to a baffle (13), and a damping strip (14) is symmetrically fixedly connected to the baffle (13). The damping strip (14) is in contact with the inner wall of the mounting groove (2).

4. A compact cell culture device according to claim 3, characterized in that: The sliding groove (11) is slidably connected to a limiting block (15), and the limiting block (15) extends above the base (1). The base (1) is symmetrically provided with fixing grooves (16).

5. A compact cell culture device according to claim 4, characterized in that: A grid plate (17) is fixedly connected inside the base (1). The grid plate (17) is located below the petri dish (5), and a reset spring (10) passes through the grid plate (17). A cooling fan (18) is rotatably connected to the bottom of the grid plate (17).

6. A compact cell culture device according to claim 5, characterized in that: One end of the cooling fan (18) is fixedly connected to a drive assembly (19), which is fixedly connected to the bottom of the base (1). A heating plate (20) is provided inside the fixing groove (16).

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