Fluorescent camera applied to inverted microscope

Abstract

The utility model provides a fluorescent camera applied to an inverted microscope. The fluorescent camera comprises a mounting base and a moving assembly, the mounting base is provided with a mounting through groove, the mounting through groove is used for mounting the focus-shrinking photosensitive assembly, and the focus-shrinking photosensitive assembly is used for adjusting the focal length of optical imaging; the moving assembly is provided with a light path adjusting element, and the moving assembly is horizontally and movably mounted at the top of the mounting base; when the moving assembly moves to the position above the focus-shrinking photosensitive assembly, the light path adjusting element is added into an optical path between the objective lens and the ocular lens. The utility model has the technical effects of simple operation, convenient installation, and neat and beautiful appearance.

Description

Technical Field

[0001] This utility model relates to microscopes, and more specifically, to a fluorescence camera used in an inverted microscope. Background Technology

[0002] Currently, in biological research, fluorescence cameras and inverted microscopes are often used in combination. The inverted microscope serves as an observation platform, providing clear images of cells, while the fluorescence camera captures fluorescence signals in these images, thereby revealing more information about the inside of the cells.

[0003] However, there is no dedicated fluorescence camera for inverted microscopes in the current technology. For example, the Nikon-ECLIPSE-Ts2 inverted microscope usually has a trinocular adapter. When using the electronic eyepiece for imaging, a special adapter needs to be found and equipped to connect the electronic eyepiece to the microscope's C-mount, which leads to a complex structure. In addition, some trinocular adapters use 50:50 beam splitting, resulting in severe loss of imaging optical path and poor fluorescence imaging effect. Furthermore, the focal length of the camera connected to the C-mount cannot be adjusted, making it difficult to achieve synchronization when used with the inverted microscope. That is, the digital image and the view through the eyepiece cannot be clear at the same time, which makes it inconvenient to use, requires multiple assembly, and involves many items. Utility Model Content

[0004] Therefore, in order to solve the problems of complex connection structure between microscope and fluorescence camera, inability to adjust focus, and inconvenience of use, this utility model provides a fluorescence camera for inverted microscopes, the specific technical solution of which is as follows:

[0005] A fluorescence camera for use in an inverted microscope, comprising a mounting base and a moving assembly;

[0006] The mounting base is provided with a mounting slot for mounting a zoom-sensing component, which is used to adjust the focal length of optical imaging.

[0007] The movable component is equipped with an optical path adjustment element, and the movable component is horizontally mounted on the top of the mounting base;

[0008] When the moving component moves above the zoom-sensing component, the optical path adjustment element enters the optical path between the objective lens and the eyepiece.

[0009] The aforementioned fluorescence camera for use in an inverted microscope achieves optical path switching by horizontal movement of the moving component 2, which in turn moves the optical path adjustment component 33 into or out of the optical path between the objective lens and the eyepiece. By installing this fluorescence camera on the inverted microscope, it can be used directly without the need for other accessories. At the same time, the focus-reducing photosensitive component 3 enables the fluorescence camera to adjust the focal length, allowing for quick synchronization of the camera and eyepiece for people with different vision. The use of the fluorescence camera on the microscope is simple to operate, thus solving the problems of complex connection structure between the microscope and the fluorescence camera in the prior art, as well as the inability to adjust the focal length and the inconvenience of use.

[0010] Furthermore, the optical path adjustment element includes a reflecting prism, a beam splitter prism, or a reflector.

[0011] Furthermore, the moving component includes an optical path adjustment element and a limiting component;

[0012] The limiting component is fixed to the top of the mounting base, and the optical path adjustment element is movably mounted on the limiting component, so that the optical path adjustment element moves along the direction of the slot opening that is close to or far away from the mounting through slot.

[0013] The optical path adjustment element is provided with an optical channel, the optical path adjustment element is installed in the optical channel, and the optical path adjustment element faces the direction of the objective lens, for changing the optical path between the objective lens and the eyepiece.

[0014] Furthermore, the number of the limiting components is two, and the two limiting components are respectively installed on both sides of the opening of the mounting through groove;

[0015] The limiting component includes a limiting block and a limiting guide post, wherein the extending direction of the limiting guide post is perpendicular to the optical path between the objective lens and the eyepiece.

[0016] The optical path adjustment element is provided with a movable through slot, the limiting guide post passes through the movable through slot, and the limiting block is fixedly installed at both ends of the limiting guide post, so that the optical path adjustment element can move between the two limiting blocks.

[0017] Furthermore, the optical path adjustment element is also provided with a limiting through groove, the extending direction of the limiting through groove is perpendicular to the extending direction of the moving through groove, and the limiting through groove is connected to the moving through groove.

[0018] The limiting through groove is provided with a compression spring and a limiting steel ball. The compression spring is fixed to the limiting through groove, and the limiting steel ball is located between the compression spring and the limiting guide post.

[0019] The limiting guide post has a notch, which is used to limit the limiting steel ball.

[0020] Furthermore, the zoom-sensing component includes a zoom lens and a photosensitive chip;

[0021] The reducing mirror is fixed to the mounting slot, and the photosensitive chip is vertically mounted below the reducing mirror via an adjustment component.

[0022] Furthermore, the adjustment assembly includes an adjustment guide post, an adjustment screw, and an adjustment platform, with the photosensitive chip fixedly mounted on the adjustment platform;

[0023] The adjusting guide post is vertically installed in the mounting slot, the adjusting screw is installed on the adjusting guide post, and the adjusting platform and the adjusting screw are connected by a thread. By rotating the adjusting screw, the adjusting platform is moved along the length direction of the adjusting screw.

[0024] Furthermore, the housing of the mounting base is adapted to the shape of the insert of the inverted microscope.

[0025] Furthermore, the mounting base includes a first base and a second base, which overlap each other to form the mounting through groove;

[0026] The first base is equipped with a USB interface and a Wi-Fi transmission interface, and the bottom of the second base is equipped with an HDMI port and a network port;

[0027] The bottom of the second base is also provided with multiple fixing holes, which are used to fix the fluorescence camera to the inverted microscope. Attached Figure Description

[0028] The present invention can be further understood from the following description taken in conjunction with the accompanying drawings. The components in the drawings are not necessarily drawn to scale; rather, the focus is on illustrating the principles of the embodiments. In different views, the same reference numerals designate corresponding parts.

[0029] Figure 1 This is a schematic diagram of the structure of the optical path adjustment component of the fluorescence camera applied to an inverted microscope when it exits the optical path between the objective lens and the eyepiece, according to an embodiment of the present invention.

[0030] Figure 2 This is a cross-sectional schematic diagram of the optical path adjustment component of the fluorescence camera used in an inverted microscope according to an embodiment of the present invention, when it is added to the optical path between the objective lens and the eyepiece;

[0031] Figure 3 This is a cross-sectional schematic diagram of the limiting steel ball of the fluorescence camera applied to the inverted microscope as described in one embodiment of the present invention, when the ball is limited in the notch.

[0032] Figure 4This is a schematic diagram of the structure of a fluorescence camera applied to an inverted microscope according to an embodiment of the present invention.

[0033] Explanation of reference numerals in the attached figures:

[0034] 1-Mounting base, 10-Mounting slot, 11-First base, 111-USB interface, 112-Wifi transmission interface, 12-Second base, 121-HDMI port, 122-Ethernet port, 123-Fixing hole; 2-Moving component, 20-Optical path adjustment element, 21-Optical path adjustment element, 210-Optical channel, 211-Moving slot, 212-Limiting slot, 213-Compression spring, 214-Limiting ball, 22-Limiting component, 221-Limiting block, 222-Limiting guide post, 2221-Notch; 3-Focus-reducing photosensitive component, 31-Reducing lens, 32-Photosensitive chip, 33-Adjustment component, 331-Adjustment guide post, 332-Adjustment screw, 333-Adjustment platform. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of this utility model clearer, the following detailed description is provided in conjunction with its embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the utility model and do not limit its scope of protection.

[0036] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly attached to the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0037] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0038] In this utility model, "first" and "second" do not represent a specific quantity or order, but are merely used to distinguish names.

[0039] like Figure 1-4As shown, a fluorescence camera for an inverted microscope according to one embodiment of the present invention includes: a mounting base 1 and a moving component 2; the mounting base 1 is provided with a mounting slot 10 for mounting a focal-reducing photosensitive component 3, which is used to adjust the focal length of optical imaging; the moving component 2 is provided with an optical path adjustment element 20, and the moving component 2 is horizontally mounted on the top of the mounting base 1; when the moving component 2 moves above the focal-reducing photosensitive component 3, the optical path adjustment element 20 enters the optical path between the objective lens and the eyepiece.

[0040] The fluorescence camera of this invention is applied to an inverted microscope. Specifically, the fluorescence camera is installed between the objective lens and the eyepiece of the microscope to switch the optical path between the objective lens and the eyepiece. The optical path includes a fully transparent optical path or a total internal reflection optical path. The fully transparent optical path is the optical path where the optical path adjustment element 20 is removed from the optical path between the objective lens and the eyepiece, that is, the original optical path between the objective lens and the eyepiece is not damaged, and the imaging effect under the eyepiece is not affected. The total internal reflection optical path is the optical path where the optical path adjustment element 20 is added to the optical path between the objective lens and the eyepiece, that is, the optical path is connected to the fluorescence camera using the optical path adjustment element 20, thereby increasing the image brightness of the fluorescence and achieving a better imaging effect.

[0041] In this invention, the switching of the optical path is achieved by the horizontal movement of the moving component 2, which drives the optical path adjustment component 33 to join or leave the optical path between the objective lens and the eyepiece. By installing the fluorescence camera on an inverted microscope, it can be used directly without the need for other accessories. At the same time, the focal length adjustment function of the focusing photosensitive component 3 enables the fluorescence camera to adjust the focal length, allowing people with different vision to quickly adjust the synchronization between the camera and the eyepiece. The use of the fluorescence camera on the microscope is simple to operate, thus solving the problems of complex connection structure between the microscope and the fluorescence camera in the prior art, as well as the inability to adjust the focal length and the inconvenience of use.

[0042] In one embodiment, the optical path adjustment element 20 includes a reflecting prism, a beam splitter, or a mirror. Specifically, when the fluorescence camera is applied to the Nikon-ECLIPSE-Ts2 inverted microscope, the optical path adjustment element 20 is preferably a reflecting prism. When the reflecting prism is added to the optical path between the objective lens and the eyepiece, the light from the objective lens enters the reflecting prism and is refracted by 90° before entering the mounting slot 10. After the focal length is adjusted by the focusing photosensitive component 3, optical imaging is performed.

[0043] In one embodiment, the moving component 2 includes an optical path adjustment element 21 and a limiting component 22; the limiting component 22 is fixed to the top of the mounting base 1, and the optical path adjustment element 21 is movably mounted on the limiting component 22, so that the optical path adjustment element 21 moves along the direction close to or away from the slot of the mounting through slot 10; the optical path adjustment element 21 is provided with an optical channel 210, and the optical path adjustment element 20 is mounted in the optical channel 210, and the optical path adjustment element 20 faces the direction of the objective lens, for changing the optical path between the objective lens and the eyepiece.

[0044] In this utility model, such as Figure 2 As shown, the optical channel 210 includes a first channel and a second channel that are perpendicular to each other. The first channel and the second channel are connected to make the optical channel 210 L-shaped. The optical path adjustment element 20 is installed between the first channel and the second channel. When the optical path adjustment element 21 moves to the top of the slot of the mounting slot 10, the second channel is connected to the mounting slot 10. After the light from the objective lens passes through the first channel, it is adjusted by the optical path adjustment element 20 and then enters the second channel. Finally, it enters the mounting slot 10 and is adjusted by the focusing photosensitive assembly 3, thereby changing the optical path between the objective lens and the eyepiece.

[0045] In one embodiment, such as Figure 1 As shown, there are two limiting components 22, which are respectively installed on both sides of the opening of the mounting slot 10. The limiting component 22 includes a limiting block 221 and a limiting guide post 222. The extension direction of the limiting guide post 222 is perpendicular to the optical path between the objective lens and the eyepiece. The optical path adjustment element 21 is provided with a moving slot 211. It can be understood that there are two moving slots 211. The limiting guide post 222 passes through the moving slot 211, and the two ends of the limiting guide post 222 are fixedly installed with limiting blocks 221, so that the optical path adjustment element 21 can move between the two limiting blocks 221. The limiting blocks 221 of the two limiting components 22 play a limiting role in the movement of the optical path adjustment element 21.

[0046] In one embodiment, the optical path adjustment element 21 is further provided with a limiting through groove 212, the extending direction of the limiting through groove 212 is perpendicular to the extending direction of the moving through groove 211, and the limiting through groove 212 is connected to the moving through groove 211; a compression spring 213 and a limiting steel ball 214 are provided in the limiting through groove 212, the compression spring 213 is fixed in the limiting through groove 212, and the limiting steel ball 214 is located between the compression spring 213 and the limiting guide post 222; the limiting guide post 222 is provided with a notch 2221, the notch 2221 is used to limit the limiting steel ball 214.

[0047] In this utility model, such as Figure 3As shown, during optical path switching, the compression spring 213 interacts with the limiting guide post 222, thereby pressing against the limiting steel ball 214. When the moving component 2 moves to the corresponding optical path, the limiting steel ball 214 falls into the recess 2221 of the limiting guide post 222, achieving self-locking of the moving component 2. It can be understood that in this embodiment, one limiting guide post 222 has two recesses 2221, and the distance between the two recesses 2221 corresponds to the moving distance of the moving component 2. Specifically, the compression spring 213 is fixed to the limiting through groove 212 by fastening screws.

[0048] In one embodiment, the zoom-in photosensitive assembly 3 includes a zoom lens 31 and a photosensitive chip 32; the zoom lens 31 is fixed to the mounting slot 10, and the photosensitive chip 32 is vertically mounted below the zoom lens 31 by an adjustment assembly 33.

[0049] In this invention, the reducing mirror 31 is used to focus the image light transmitted through the objective lens and the optical path adjustment element 20 onto the photosensitive chip 32, which then converts the image light into electrical signals to ultimately form a digital image. Furthermore, the focus length can be adjusted by regulating the distance between the photosensitive chip 32 and the reducing mirror 31.

[0050] In one embodiment, the adjustment assembly 33 includes an adjustment guide post 331, an adjustment screw 332, and an adjustment platform 333, with the photosensitive chip 32 fixedly mounted on the adjustment platform 333;

[0051] The adjusting guide post 331 is vertically installed in the mounting slot 10, the adjusting screw 332 is installed on the adjusting guide post 331, and the adjusting table 333 and the adjusting screw 332 are connected by a thread. By rotating the adjusting screw 332, the adjusting table 333 is moved along the length of the adjusting screw 332.

[0052] Specifically, such as Figure 2 As shown, the adjusting guide post 331 is provided with an adjusting through groove, the adjusting screw 332 is installed in the adjusting through groove, the adjusting platform 333 is sleeved on the adjusting screw 332, and the adjusting platform 333 and the adjusting screw 332 are connected by threads. In some possible embodiments, the adjusting screw 332 is also provided with an adjusting knob, and the adjusting screw 332 is rotated by turning the adjusting knob, thereby driving the adjusting platform 333 to move.

[0053] In one embodiment, the housing of the mounting base 1 is adapted to the shape of the inverted microscope insert. Specifically, if the fluorescence camera is applied to the Nikon-ECL IPSE-Ts2 inverted microscope, the housing of the mounting base 1 is designed based on the shape of the Nikon-ECL IPSE-Ts2 inverted microscope, thereby making the overall appearance of the microscope neat and beautiful.

[0054] In one embodiment, the mounting base 1 includes a first base 11 and a second base 12, which overlap each other to form a mounting through groove 10. The first base 11 is provided with a USB interface 111 and a Wi-Fi transmission interface 112, and the bottom of the second base 12 is provided with an HDMI port 121 and a network port 122. Specifically, the USB interface 111, the Wi-Fi transmission interface 112, the HDMI port 121, and the network port 122 are used for image and signal transmission, thereby achieving the effect of electronic imaging.

[0055] In addition, such as Figure 4 As shown, a plurality of fixing holes 123 are provided at the bottom of the second base 12. The fixing holes 123 are used to fix the fluorescence camera to the inverted microscope. In this utility model, the way the fluorescence camera is fixed to the inverted microscope is the same as the way the microscope insert is fixed, that is, it is achieved by the cooperation of M3 screws with the fixing holes 123, so that the installation of the fluorescence camera on the microscope is simple and quick. In some embodiments, the number of fixing holes 123 is 3, and they are triangular at the bottom of the second base 12 to improve the stability of the fluorescence camera fixing.

[0056] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0057] The embodiments described above are merely illustrative of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. A fluorescence camera for use in an inverted microscope, characterized in that, include: Mounting base and moving components; The mounting base is provided with a mounting slot for mounting a zoom-sensing component, which is used to adjust the focal length of optical imaging. The movable component is equipped with an optical path adjustment element, and the movable component is horizontally mounted on the top of the mounting base; When the moving component moves above the zoom-sensing component, the optical path adjustment element enters the optical path between the objective lens and the eyepiece.

2. The fluorescence camera for use in an inverted microscope according to claim 1, characterized in that, The optical path adjustment element includes a reflecting prism, a beam splitter prism, or a reflector.

3. The fluorescence camera for use in an inverted microscope according to claim 1, characterized in that, The moving component includes an optical path adjustment element and a limiting component; The limiting component is fixed to the top of the mounting base, and the optical path adjustment element is movably mounted on the limiting component, so that the optical path adjustment element moves along the direction of the slot opening that is close to or far away from the mounting through slot. The optical path adjustment element is provided with an optical channel, the optical path adjustment element is installed in the optical channel, and the optical path adjustment element faces the direction of the objective lens, for changing the optical path between the objective lens and the eyepiece.

4. The fluorescence camera for use in an inverted microscope according to claim 3, characterized in that, The number of the limiting components is two, and the two limiting components are respectively installed on both sides of the slot of the mounting through groove; The limiting component includes a limiting block and a limiting guide post, wherein the extending direction of the limiting guide post is perpendicular to the optical path between the objective lens and the eyepiece. The optical path adjustment element is provided with a movable through slot, the limiting guide post passes through the movable through slot, and the limiting block is fixedly installed at both ends of the limiting guide post, so that the optical path adjustment element can move between the two limiting blocks.

5. The fluorescence camera for use in an inverted microscope according to claim 4, characterized in that, The optical path adjustment element is further provided with a limiting groove, the extension direction of the limiting groove is perpendicular to the extension direction of the moving groove, and the limiting groove is connected to the moving groove. The limiting through groove is provided with a compression spring and a limiting steel ball. The compression spring is fixed to the limiting through groove, and the limiting steel ball is located between the compression spring and the limiting guide post. The limiting guide post has a notch, which is used to limit the limiting steel ball.

6. The fluorescence camera for use in an inverted microscope according to claim 1, characterized in that, The zoom-sensing component includes a zoom lens and a photosensitive chip; The reducing mirror is fixed to the mounting slot, and the photosensitive chip is vertically mounted below the reducing mirror via an adjustment component.

7. The fluorescence camera for use in an inverted microscope according to claim 6, characterized in that, The adjustment assembly includes an adjustment guide post, an adjustment screw, and an adjustment platform, and the photosensitive chip is fixedly mounted on the adjustment platform; The adjusting guide post is vertically installed in the mounting slot, the adjusting screw is installed on the adjusting guide post, and the adjusting platform and the adjusting screw are connected by a thread. By rotating the adjusting screw, the adjusting platform is moved along the length direction of the adjusting screw.

8. The fluorescence camera for use in an inverted microscope according to claim 1, characterized in that, The housing of the mounting base is adapted to the shape of the inverted microscope insert.

9. The fluorescence camera for an inverted microscope according to claim 8, characterized in that, The mounting base includes a first base and a second base, which overlap each other to form the mounting through groove. The first base is equipped with a USB port and a Wi-Fi transmission port, while the second base is equipped with an HDMI port and a network port at the bottom. The bottom of the second base is also provided with multiple fixing holes, which are used to fix the fluorescence camera to the inverted microscope.

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