Agarose gel preparation device
By designing an automated agarose gel preparation device, the problem of high operational risks in agarose gel preparation was solved, and a convenient and safe preparation process was achieved.
Patent Information
- Application Number
- CN202520492299.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-19
- Publication Date
- 2026-02-13
- Estimated Expiration
- 2035-03-19
AI Technical Summary
The preparation of agarose gel in the existing technology has the problem of high operation risk, easy burns and contact with toxic buffer solutions.
An agarose gel preparation device was designed, comprising a mixing chamber, a stirring rod, a heating module, a buffer supply module, and a pipette nozzle. The device avoids manual contact with the buffer solution through automated stirring and heating, and enhances safety by employing a semiconductor cooling chip and a solenoid valve.
This method enables convenient and safe preparation of agarose gels, avoiding the risks of burns and contact with buffer solutions, and improving the ease and safety of operation.
Smart Images
Figure CN223901804U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to agarose gel preparation technical field, especially an agarose gel preparation device. BACKGROUND
[0002] The principle of nucleic acid gel electrophoresis is based on the migration behavior of charged nucleic acid molecules in an electric field. When nucleic acid molecules are placed in an electric field, they will move towards the appropriate electrode, and the speed of this movement is called electrophoretic mobility; the mobility is directly proportional to the electric field strength and the number of net charges carried by the electrophoretic molecules, that is, the greater the electric field strength and the more the number of net charges carried by the electrophoretic molecules, the faster the migration speed; nucleic acid electrophoresis is usually carried out in agarose gel.
[0003] In the prior art, agarose gel is mostly prepared by manual method, that is, a certain amount of agarose is weighed, a certain amount of buffer solution is measured, and then the agarose and the buffer solution are manually stirred, and then the stirred liquid is sent into a microwave oven for heating and melting, and finally the liquid after heating and melting is poured out and cooled and condensed; since the buffer solution has certain stimulation and toxicity, there is certain danger if it is accidentally touched during operation, and the agarose glue liquid may be violently boiled during heating, which may cause burns during operation, and the preparation of agarose gel by manual method has certain limitations, and an agarose gel preparation device is urgently needed. UTILITY MODEL CONTENT
[0004] The utility model aims at the deficiencies of the prior art and provides an agarose gel preparation device, which is more convenient to operate as a whole, can effectively avoid touching the buffer solution and causing burns, and is more convenient and safe in the whole preparation process, and can be well used for the preparation of agarose gel.
[0005] In order to achieve the above-mentioned purpose, the utility model adopts the technical scheme of an agarose gel preparation device, which comprises a stirring bin, a stirring rod rotatingly connected in the stirring bin in the vertical direction, a stirring power mechanism for driving the stirring rod to rotate, a heating module for heating the stirring bin, a buffer solution supply module, and a pipette nozzle; the top of the stirring bin is provided with a liquid inlet for communicating with the buffer solution supply module and a feed inlet for feeding agarose; the bottom of the stirring bin is provided with a liquid outlet for communicating with the pipette nozzle, and a liquid outlet valve is connected between the liquid outlet and the pipette nozzle.
[0006] Further improvement of the above scheme is that the buffer solution supply module comprises a buffer solution tank and a liquid pump, the liquid pump comprises a liquid suction port and a liquid delivery port, the liquid suction port is connected with the buffer solution tank, a switch valve is connected in series between the liquid suction port and the buffer solution tank, the liquid delivery port is connected with the liquid inlet, and a liquid inlet valve is connected in series between the liquid delivery port and the liquid inlet.
[0007] Further improvement of the above scheme is that the liquid inlet valve and the liquid outlet valve are respectively electromagnetic valves.
[0008] Further improvement of the above scheme is that the heating module comprises a semiconductor refrigeration sheet arranged beside the uniform stirring bin.
[0009] Further improvement of the above scheme is that the semiconductor refrigeration sheet comprises two semiconductor refrigeration sheets, and the two semiconductor refrigeration sheets are respectively arranged at the front end and the rear end of the uniform stirring bin.
[0010] Further improvement of the above scheme is that the stirring rod comprises a horizontal stirring part and two vertical stirring parts arranged at the two sides of the horizontal stirring part.
[0011] Further improvement of the above scheme is that a feeding funnel is arranged at the position of the feeding port.
[0012] Further improvement of the above scheme is that an insulation box is arranged outside the uniform stirring bin.
[0013] The agarose gel preparation device has the advantages that: the agarose gel preparation device comprises a uniform stirring bin, a stirring rod rotatingly connected in the uniform stirring bin in a vertical direction, a stirring power mechanism for driving the stirring rod to rotate, a heating module for heating the uniform stirring bin, a buffer solution supply module, and a liquid transfer gun nozzle, a liquid inlet for being connected with the buffer solution supply module and a feeding port for feeding agarose are arranged on the top of the uniform stirring bin, a liquid outlet for being connected with the liquid transfer gun nozzle is arranged on the bottom of the uniform stirring bin, and a liquid outlet valve is connected in series between the liquid outlet and the liquid transfer gun nozzle.
[0014] When the agarose gel is prepared by the agarose gel preparation device, the buffer solution is added to the buffer solution supply module in advance, the buffer solution is added to the uniform stirring bin by the buffer solution supply module, the agarose is added to the uniform stirring bin through the feeding port, the stirring rod is driven to rotate by the stirring power mechanism, the agarose and the buffer solution are automatically stirred, the uniform stirring bin is heated by the heating module, and finally the liquid after being heated and melted is guided out of the liquid transfer gun nozzle for cooling and condensation. BRIEF DESCRIPTION OF DRAWINGS
[0015] Fig. 1The utility model discloses a structure schematic drawing.
[0016] Fig. 2 The utility model discloses a structure schematic drawing of the stirring bin.
[0017] The figure mark explains: stirring bin 1, stirring rod 2, horizontal stirring part 21, vertical stirring part 22, stirring power mechanism 3, heating module 4, semiconductor refrigerating sheet 41, buffer solution liquid supply module 5, buffer solution tank 51, liquid pump 52, liquid pumping port 521, liquid sending port 522, switch valve 53, liquid inlet valve 54, pipette nozzle 6, liquid inlet 7, feed inlet 8, liquid outlet 9, liquid outlet valve 10, feed hopper 11, heat preservation box 12. Specific implementation:
[0018] The utility model makes further explanation from the following combined with the drawing, as shown in the utility model discloses a structure schematic drawing. Figs. 1-2 The utility model discloses a structure schematic drawing of the stirring bin.
[0019] The buffer solution liquid supply module 5 includes a buffer solution tank 51 and a liquid pump 52.
[0020] The liquid inlet valve 54 and the liquid outlet valve 10 are electromagnetic valves, and the automation degree can be further improved, and the practicability is stronger.
[0021] The heating module 4 comprises a semiconductor refrigerating sheet 41 arranged beside the stirring bin 1, and the stirring bin 1 can be well heated by the semiconductor refrigerating sheet 41, so as to replace the previous heating by a microwave oven.
[0022] Compared with the semiconductor refrigerating sheet 41 with only one, the semiconductor refrigerating sheet 41 has two, and the two semiconductor refrigerating sheets 41 are located at the front and rear ends of the stirring bin 1, so that the heating efficiency is higher, and the overall heating is more uniform.
[0023] The stirring rod 2 comprises a horizontal stirring part 21 and two vertical stirring parts 22 located at the two sides of the horizontal stirring part 21, so that the stirring is more uniform.
[0024] The feeding funnel 11 is arranged at the position of the feeding port 8, so that the agarose is more conveniently fed.
[0025] The outer side of the stirring bin 1 is covered with the heat preservation box 12, so that the heat loss can be well prevented, and the practicability is stronger.
[0026] Working principle:
[0027] When the agarose gel is prepared by the utility model, the buffer solution is added into the buffer solution supply module 5 in advance, the buffer solution is added into the stirring bin 1 by the buffer solution supply module 5, the agarose is added into the stirring bin 1 through the feeding port 8, the stirring rod 2 is driven to rotate by the stirring power mechanism 3, and the agarose and the buffer solution are automatically stirred, the stirring bin 1 is heated by the heating module 4, and finally the liquid after heating and melting is guided out of the cooling condensation through the pipette nozzle 6; compared with the previous manual preparation of the agarose gel, the utility model not only has more convenient overall operation, but also can effectively avoid touching the buffer solution and causing scalding, so that the whole preparation process is more convenient and safe, and the utility model can be well used for the preparation of the agarose gel.
[0028] Of course, the above-mentioned is only the preferred embodiment of the utility model, so that the equivalent changes or modifications of the structure, features and principles described in the utility model patent application range are included in the utility model patent application range.
Claims
1. An apparatus for preparing an agarose gel, characterized by: The device comprises a stirring bin (1), a stirring rod (2) connected to the stirring bin (1) in the vertical direction, a stirring power mechanism (3) for driving the stirring rod (2) to rotate, a heating module (4) for heating the stirring bin (1), a buffer solution supply module (5), and a pipette nozzle (6). The top of the stirring bin (1) is provided with a liquid inlet (7) for communicating with the buffer solution supply module (5) and a material inlet (8) for placing agarose. The bottom of the stirring bin (1) is provided with a liquid outlet (9) for communicating with the pipette nozzle (6). The liquid outlet (9) and the pipette nozzle (6) are connected in series with a liquid outlet valve (10).
2. An apparatus for preparing an agarose gel according to claim 1, characterized in that: The buffer solution supply module (5) comprises a buffer solution tank (51) and a liquid pump (52). The liquid pump (52) comprises a liquid suction port (521) and a liquid delivery port (522). The liquid suction port (521) is connected to the buffer solution tank (51). The liquid suction port (521) and the buffer solution tank (51) are connected in series with a switch valve (53). The liquid delivery port (522) is connected to the liquid inlet (7). The liquid delivery port (522) and the liquid inlet (7) are connected in series with a liquid inlet valve (54).
3. An apparatus for preparing an agarose gel according to claim 2, characterized in that: The liquid inlet valve (54) and the liquid outlet valve (10) are electromagnetic valves.
4. The apparatus of claim 1, wherein: The heating module (4) comprises semiconductor refrigerating sheets (41) arranged beside the stirring bin (1).
5. An apparatus for preparing an agarose gel according to claim 4, characterized in that: The semiconductor refrigerating sheets (41) are two, and the two semiconductor refrigerating sheets (41) are respectively located at the front and rear ends of the stirring bin (1).
6. The apparatus of claim 1, wherein: The stirring rod (2) comprises a horizontal stirring part (21) and two vertical stirring parts (22) respectively located on both sides of the horizontal stirring part (21).
7. The apparatus of claim 1, wherein: The material inlet (8) is provided with a material funnel (11).
8. The apparatus of claim 1, wherein: The stirring bin (1) is covered with a heat preservation box (12) on the outside.