Pathogen detection and analysis system for metagenome sequencing data

Abstract

The utility model discloses a pathogen detection and analysis system for metagenome sequencing data, which is designed according to a paramagnetic particle method for extracting nucleic acid, and is simple in structure, cost-saving and ingenious in conception. The system comprises a detection and analysis equipment body and a nucleic acid extraction device, the nucleic acid extraction device can simply, automatically and efficiently complete the process of magnetic bead method nucleic acid extraction through a horizontally movable placing frame containing a sample tube and a vertically movable and detachable magnetic rod assembly, the whole nucleic acid extraction process does not need manual operation of workers, the extraction efficiency is higher, and the detection and analysis efficiency is improved. Errors are smaller, taking is more convenient, and time and labor are saved.

Description

TECHNICAL FIELD

[0001] The utility model relates to detection analysis technical field especially, relate to a kind of pathogen detection analysis system of macrogenomic sequencing data. BACKGROUND

[0002] Metagenomics sequencing is a high-throughput DNA sequencing technology, aiming to analyze the genomic information of all microorganisms in complex microbial communities. Unlike traditional single-microbe genome sequencing, metagenomics sequencing sequences the DNA of the entire microbial community, including bacteria, archaea, eukaryotic microorganisms, etc.

[0003] Based on modern high-throughput sequencing technology, the first step is to extract total DNA from environmental samples and sequence these DNA fragments using high-throughput sequencing technology, such as Illumina's next-generation sequencing technology. This step generates a large number of short DNA fragment sequences, each containing a portion of the microbial genome. The large number of short DNA fragment sequences obtained by sequencing need to be processed and analyzed to determine the species identity of the microorganisms and reveal the metabolic pathways and potential functions of the microbial community.

[0004] Current pathogen detection analysis systems for metagenomic sequencing data generally involve manual extraction of nucleic acids by technicians. After manual extraction, the extracted samples are placed into the detection analysis equipment for detection. Manual extraction is inefficient and prone to errors. Large-scale automated nucleic acid extractors (automatic liquid workstations) can operate automatically, but they are very expensive and time-consuming, with each nucleic acid extraction taking more than an hour, resulting in low efficiency. UTILITY MODEL CONTENT

[0005] The utility model provides a simple, efficient, and low-cost pathogen detection analysis system for metagenomic sequencing data to address the above technical problems.

[0006] To achieve the above purpose, the utility model provides the following technical solutions:

[0007] A pathogen detection analysis system for metagenomic sequencing data includes a detection analysis equipment body, a nucleic acid extraction device is fixedly connected to one side of the detection analysis equipment body, the nucleic acid extraction device includes an extraction box, a placement frame is movably arranged horizontally inside the lower side of the extraction box, a tube rack is movably connected inside the placement frame, and a plurality of sample tubes are inserted into the tube rack.

[0008] Further, the placing frame is horizontally movable through a horizontal driving assembly, which comprises a threaded rod, a limiting block and a servo motor, both ends of the threaded rod are installed in a positioning frame through bearings, the positioning frame is fixedly connected with the body of the detection and analysis equipment through a fixing seat, one end of the threaded rod is fixedly connected with the output end of the servo motor, the outer surface of the threaded rod is threadedly connected with the limiting block, and the limiting block is fixedly installed on the lower surface of the placing frame.

[0009] Further, the extraction box is fixedly installed on the positioning frame.

[0010] Further, the bottom of the positioning frame is connected with a support.

[0011] Further, the magnetic rod assembly is movable up and down through an up-down driving assembly, which comprises a servo cylinder and a positioning seat, the servo cylinder is fixed on the top of the extraction box, the output end of the servo cylinder is fixedly connected with the upper surface of the positioning seat, and the lower surface of the positioning seat is movably connected with the magnetic rod assembly.

[0012] Further, the positioning seat is slidably connected with the inner wall of the extraction box through a sliding block, the two sides of the positioning seat are fixedly connected with the sliding blocks, and the two sides of the inner wall of the extraction box are provided with limiting grooves, and the sliding blocks are slidably connected in the limiting grooves.

[0013] Further, the magnetic rod assembly comprises a magnetic rod, a positioning plate and a positioning handle, a plurality of magnetic rods are connected with the lower surface of one positioning plate in a column along the Y-axis direction, a plurality of positioning plates are sequentially inserted into the lower surface of the positioning seat along the X-axis direction, and one positioning plate is fixedly connected with the positioning seat through one positioning handle.

[0014] Further, the fixing seat and the positioning frame are fixedly connected through a locking handle.

[0015] Further, the placing frame is provided with two or more than two.

[0016] Further, the placing frame is provided with a limiting groove, and the tube rack is inserted into the placing frame.

[0017] Compared with the prior art, the macro genome sequencing data pathogen detection and analysis system has the advantages that:

[0018] The macro genome sequencing data pathogen detection and analysis system has the advantages that: the magnetic bead method nucleic acid extraction is designed, the structure is simple, the cost is saved, and the design is ingenious. The placing frame horizontally movable and the magnetic rod assembly movable up and down and detachable are used to simply, automatically and efficiently complete the process of magnetic bead method nucleic acid extraction, the whole nucleic acid extraction process does not need manual operation of workers, the extraction efficiency is higher, the error is smaller, the taking is more convenient, and time and labor are saved. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments described in the present application, and other drawings can also be obtained by those skilled in the art according to these drawings.

[0020] Figure 1 The isometric structural schematic view of the pathogen detection and analysis system for metagenomic sequencing data provided by the embodiment of the present application;

[0021] Figure 2 The right isometric structural schematic view of the pathogen detection and analysis system for metagenomic sequencing data provided by the embodiment of the present application;

[0022] Figure 3 The bottom isometric structural schematic view of the pathogen detection and analysis system for metagenomic sequencing data provided by the embodiment of the present application;

[0023] Figure 4 The cross-sectional isometric structural schematic view of the pathogen detection and analysis system for metagenomic sequencing data provided by the embodiment of the present application;

[0024] Figure 5 The first local enlarged structural schematic view of the pathogen detection and analysis system for metagenomic sequencing data provided by the embodiment of the present application;

[0025] Figure 6 The second local enlarged structural schematic view of the pathogen detection and analysis system for metagenomic sequencing data provided by the embodiment of the present application;

[0026] Figure 7 The third local enlarged structural schematic view of the pathogen detection and analysis system for metagenomic sequencing data provided by the embodiment of the present application.

[0027] Explanation of reference signs:

[0028] 1, detection and analysis equipment body; 2, fixing seat; 3, positioning frame; 4, threaded rod; 5, servo motor; 6, limiting block; 7, placing frame; 8, pipe rack; 9, extraction box; 10, servo cylinder; 11, positioning seat; 12, sliding block; 13, limiting groove; 14, support; 15, positioning plate; 16, positioning handle; 17, magnetic bar; 18, sample tube; 19, locking handle. DETAILED DESCRIPTION

[0029] In order to make the technical scheme of the utility model better understood by the skilled in the art, the utility model will be further described in detail below in conjunction with the drawings. The following described embodiments are only specific embodiments of the present application, which are used to illustrate the technical scheme of the present application, but not limit the present application, the protection scope of the present application is not limited to this.

[0030] Referring to Figures 1-7 As shown in the figure;

[0031] The utility model provides a kind of pathogen detection analysis system of macro genome sequencing data, including detection analysis equipment body 1, the one side of detection analysis equipment body 1 is fixedly connected with nucleic acid extraction device, and the nucleic acid extraction device includes extraction box 9, specifically, extraction box 9 is fixedly installed on positioning frame 3. Specifically, to facilitate disassembly, the way of plug-in can be used. Meanwhile, to make the connection more firm, the fixed seat 2 and positioning frame 3 are fixedly connected by locking handle 19. The bottom of positioning frame 3 is connected with bracket 14. By the arrangement of bracket 14, positioning frame 3 can be placed more stably.

[0032] Horizontal movable placing frame 7 is arranged in the inside lower side of extraction box 9, and the placing frame 7 is provided with two or more than two, for containing sample tube 18 with different reaction liquid, and the horizontal movement can be used alternately. The inside of placing frame 7 is movably connected with tube rack 8, specifically, the inside of placing frame 7 is provided with limiting groove, and tube rack 8 is inserted in placing frame 7. Multiple sample tubes 18 are inserted in the inside of tube rack 8.

[0033] The placing frame 7 is realized horizontal movement by horizontal drive assembly, specifically, horizontal drive assembly includes threaded rod 4, limiting block 6 and servo motor 5, the both ends of threaded rod 4 are installed in positioning frame 3 by bearing, positioning frame 3 is fixedly connected with detection analysis equipment body 1 by fixed seat 2, one end of threaded rod 4 is fixedly connected with the output end of servo motor 5, and the outer surface of threaded rod 4 is screw-connected with limiting block 6, and limiting block 6 is fixedly installed on the lower surface of placing frame 7.

[0034] Magnetic rod assembly is movably arranged in the inside upper side of extraction box 9, specifically, the magnetic rod assembly is realized up-down movement by up-down drive assembly, and up-down drive assembly includes servo cylinder 10 and positioning seat 11, servo cylinder 10 is fixed on the top of extraction box 9, the output end of servo cylinder 10 is fixedly connected on the upper surface of positioning seat 11, and the lower surface of positioning seat 11 is movably connected with magnetic rod assembly. Specifically, by the arrangement of servo cylinder 10, positioning seat 11 can be controlled to move up and down, and then magnetic rod assembly moves up and down, and the nucleic acid with magnetic bead in the inside of sample tube 18 is adsorbed.

[0035] In order to ensure the stability of the positioning seat 11 up and down movement operation, the positioning seat 11 is slidably connected with the inner wall of the extraction box 9 through the sliding block 12, both sides of the positioning seat 11 are fixedly connected with the sliding block 12, both sides of the inner wall of the extraction box 9 are provided with a limiting groove 13, and the sliding block 12 is slidably connected in the limiting groove 13. When used, the positioning seat 11 is not easy to deviate in position during movement through the limiting groove 13 and the sliding block 12, so that the magnetic rod 17 is inserted into the sample tube 18.

[0036] The magnetic rod assembly comprises a plurality of longitudinally arranged magnetic rods 17, and the magnetic rod 17 corresponds to the sample tube 18. Specifically, the magnetic rod assembly comprises a magnetic rod 17, a positioning plate 15 and a positioning handle 16, a plurality of magnetic rods 17 are arranged in a column along the Y-axis direction and are connected to the lower surface of a positioning plate 15, a plurality of positioning plates 15 are sequentially inserted into the lower surface of the positioning seat 11 along the X-axis direction, and one positioning plate 15 is fixedly connected with the positioning seat 11 through one positioning handle 16. Through the arrangement of the positioning handle 16, the positioning plate 15 can be more stably installed in the positioning seat 11. When replacement or cleaning is required, the positioning handle 16 is loosened, and the column of positioning plates 15 can be pulled out from the positioning seat 11.

[0037] The process of nucleic acid extraction by magnetic bead method is as follows:

[0038] Lysis: adding lysis solution to the sample to release nucleic acid; adsorption and combination: adding magnetic beads to the sample lysis solution, and adsorbing and combining nucleic acid on the magnetic beads; washing: moving the magnetic beads and nucleic acid to the washing liquid to clean the nucleic acid impurities, and removing the nucleic acid impurities; elution: moving the magnetic beads and nucleic acid to the elution liquid, and separating the nucleic acid from the magnetic beads to obtain purified nucleic acid.

[0039] The working principle of the utility model is as follows:

[0040] When the sample needs to be extracted, the staff can place the sample tube 18 (the lysis solution is used to release nucleic acid, and then the magnetic beads are added, and the nucleic acid is adsorbed and combined on the magnetic beads) with the lysis solution and the magnetic beads in the tube rack 8, and then place the sample tube 18 in the placing frame 7. Start the servo motor 5, the servo motor 5 can drive the threaded rod 4 to rotate, and the placing frame 7 with the sample tube 18 is moved to the inside of the extraction box 9. Start the servo cylinder 10, the servo cylinder 10 can drive the positioning seat 11 to move up and down, so that the magnetic rod 17 can be inserted into the sample tube 18 and moved up and down, and the nucleic acid adsorption extraction can be carried out.

[0041] After the adsorption extraction is completed, the staff starts the servo cylinder 10 to drive the magnetic rod 17 adsorbed with the nucleic acid to move upwards, starts the servo motor 5 to make the threaded rod 4 rotate, and the sample tube 18 after extraction can be pushed out from the inside of the extraction box 9, and the sample tube 18 containing the washing liquid is placed in the next placement frame 7 and enters the extraction box 9 together. Then the servo cylinder 10 is started again to drive the magnetic rod 17 adsorbed with the nucleic acid to move downwards, so that the magnetic rod 17 adsorbed with the nucleic acid is inserted into the sample tube 18 containing the washing liquid and moves up and down, and the nucleic acid impurities are cleaned and removed from the magnetic beads and the nucleic acid. At this time, the tube rack 8 in the pushed-out placement frame 7 is replaced with the tube rack 8 containing the eluent sample tube 18.

[0042] After the washing is completed, the staff starts the servo cylinder 10 to drive the magnetic rod 17 adsorbed with the nucleic acid to move upwards, starts the servo motor 5 to make the threaded rod 4 rotate, and the sample tube 18 after washing can be pushed back from the inside of the extraction box 9, and the placement frame 7 of the sample tube 18 containing the eluent is returned to the extraction box 9. Then the servo cylinder 10 is started again to drive the magnetic rod 17 adsorbed with the nucleic acid to move downwards, so that the magnetic rod 17 adsorbed with the nucleic acid is inserted into the sample tube 18 containing the eluent and moves up and down, so that the nucleic acid is separated from the magnetic beads, and the purified nucleic acid is obtained. Thus, the entire nucleic acid extraction process is completed. The servo motor 5 is started to push out the placement frame 7 of the sample tube 18 containing the purified nucleic acid, and after the tube rack 8 in the placement frame 7 is removed, it can be transferred to the pathogen detection and analysis system of metagenomic sequencing data for nucleic acid sequence analysis. The entire nucleic acid extraction process does not require manual operation by the staff, has higher extraction efficiency, smaller error, is more convenient to take, and saves time and effort.

[0043] It should be noted that, in the present document, the terms such as first and second, etc., are merely used to differentiate one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Also, the terms "comprises", "comprising", or any other variant thereof are intended to cover non-exclusive inclusions, so that a process, method, article, or apparatus that includes a list of elements does not only include those elements, but also includes other elements not explicitly listed, or inherent to such a process, method, article, or apparatus. Without more limitations, the element defined by the statement "comprises a" does not exclude the presence of additional identical elements in the process, method, article, or apparatus that includes the element.

[0044] The above-described embodiments are merely specific implementations of the present application, and are used to illustrate the technical solutions of the present application, rather than limit the same. The protection scope of the present application is not limited thereto. Although the present application is described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that any person skilled in the art can make modifications or easily think of changes to the technical solutions recorded in the foregoing embodiments, or make equivalent replacements to part of the technical solutions within the technical scope disclosed by the present application. The modifications, changes or replacements do not cause the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present application. All should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A pathogen detection analysis system of metagenomic sequencing data, comprising a detection analysis device body (1), characterized in that, The nucleic acid extraction device is fixedly connected to one side of the detection analysis equipment body (1), and comprises an extraction box (9), a placing frame (7) movably arranged on the lower side of the extraction box (9), a pipe rack (8) movably connected to the inside of the placing frame (7), and a plurality of sample tubes (18) inserted into the pipe rack (8).

2. The pathogen detection analysis system of metagenomic sequencing data according to claim 1, wherein, The placing frame (7) is movably arranged by a horizontal driving assembly, and the horizontal driving assembly comprises a threaded rod (4), a limiting block (6) and a servo motor (5). Both ends of the threaded rod (4) are rotatably arranged in a positioning frame (3) through bearings, the positioning frame (3) is fixedly connected to the detection analysis equipment body (1) through a fixed seat (2), one end of the threaded rod (4) is fixedly connected to the output end of the servo motor (5), and the outer surface of the threaded rod (4) is threadedly connected to the limiting block (6), which is fixedly arranged on the lower surface of the placing frame (7).

3. The pathogen detection analysis system of metagenomic sequencing data according to claim 2, wherein, The extraction box (9) is fixedly arranged on the positioning frame (3).

4. The pathogen detection analysis system of metagenomic sequencing data according to claim 2, wherein, The positioning frame (3) is connected with a support (14) at the bottom.

5. The pathogen detection analysis system of metagenomic sequencing data according to claim 1, wherein, The magnetic rod assembly is movably arranged by an up-down driving assembly, and the up-down driving assembly comprises a servo cylinder (10) and a positioning seat (11). The servo cylinder (10) is fixedly arranged at the top of the extraction box (9), the output end of the servo cylinder (10) is fixedly connected to the upper surface of the positioning seat (11), and the lower surface of the positioning seat (11) is movably connected with the magnetic rod assembly.

6. The pathogen detection analysis system of metagenomic sequencing data according to claim 5, wherein, The positioning seat (11) is slidably connected with the inner wall of the extraction box (9) through a sliding block (12), the positioning seat (11) is fixedly connected with the sliding block (12) on both sides, and the inner wall of the extraction box (9) is provided with a limiting groove (13) on both sides.

7. The pathogen detection analysis system of metagenomic sequencing data according to claim 5, wherein, The magnetic rod assembly comprises a magnetic rod (17), a positioning plate (15) and a positioning handle (16). A plurality of magnetic rods (17) are connected to the lower surface of a positioning plate (15) in a column along the Y-axis direction, a plurality of positioning plates (15) are sequentially inserted into the lower surface of the positioning seat (11) along the X-axis direction, and one positioning plate (15) is fixedly connected with the positioning seat (11) through one positioning handle (16).

8. The pathogen detection analysis system of metagenomic sequencing data according to claim 2, wherein, The fixed seat (2) and the positioning frame (3) are fixedly connected through a locking handle (19).

9. The pathogen detection analysis system of metagenomic sequencing data according to claim 1, wherein, The placing frame (7) is provided in two or more.

10. The pathogen detection analysis system of metagenomic sequencing data according to claim 1, wherein, The placing frame (7) is provided with a limiting groove, and the pipe rack (8) is inserted into the placing frame (7).

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