Novel lateral chromatography detection card

Abstract

The utility model relates to a novel lateral chromatography detection card which comprises a bottom support, a sample adding strip positioning area, a containing groove and a display strip positioning area are sequentially arranged on the bottom support in the length direction of the bottom support, and the horizontal height of the containing groove is lower than the horizontal height of the sample adding strip positioning area and the horizontal height of the display strip positioning area; the test strip comprises a sample pad arranged on the sample adding strip positioning area, a display strip arranged on the display strip positioning area and a gold label freeze-drying device corresponding to the accommodating groove, and the sample pad and the gold label freeze-drying device are in lap joint in sequence; the upper cover covers the bottom support to form a cavity for accommodating the test strip, a sample adding hole corresponding to the sample pad and a detection window corresponding to the display strip are formed in the upper cover, and the reaction time of a detection target object and a gold-labeled antibody in a sample and the concentration of the detection target object during reaction are effectively prolonged through the accommodating groove; therefore, the detection sensitivity is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to reagent detection technical field, concretely relates to a novel lateral chromatographic detection card. BACKGROUND

[0002] At present, colloidal gold detection card detection adopts full horizontal chromatography and integration detection strip, namely sample pad, gold mark pad, chromatographic membrane, water absorption paper are connected in turn on a test paper strip, after sample is added to sample pad, sample liquid gradually chromatographs along the direction of test paper strip, and detection target in sample liquid reacts with antibody on gold mark pad.

[0003] But adopting this kind of mode, after adding sample, liquid chromatographs fast along the direction of test paper strip, and the reaction time of detection target in sample and gold mark antibody is short, leading to that detection sensitivity is much lower than other immune methods, because of fast speed, when sample liquid chromatographs to gold mark antibody, the volume of sample liquid is relatively small, so the amount of detection target in sample is less, which further influences detection sensitivity. UTILITY MODEL CONTENT

[0004] To solve the above technical problem, the utility model provides a novel lateral chromatographic detection card, which effectively improves the reaction time of detection target in sample and gold mark antibody and the concentration of detection target during reaction, thereby improving detection sensitivity.

[0005] The utility model provides a novel lateral chromatographic detection card to solve the above technical problem, which comprises,

[0006] The bottom support is provided with a sample adding strip positioning area, a containing groove and a display strip positioning area along the length direction in sequence, and the horizontal height of the containing groove is lower than that of the sample adding strip positioning area and the display strip positioning area.

[0007] The test paper strip comprises a sample pad placed on the sample adding strip positioning area, a display strip placed on the display strip positioning area and a gold mark freeze-dried corresponding to the containing groove, the sample pad and the gold mark freeze-dried are sequentially overlapped, the other side of the gold mark freeze-dried is disconnected with the display strip, and the side of the display strip close to the containing groove extends out of the display strip positioning area.

[0008] The upper cover is arranged on the bottom support to form a cavity containing the test paper strip, and the upper cover is provided with a sample adding hole corresponding to the sample pad and a detection window corresponding to the display strip.

[0009] Further, the bottom of the sample adding strip positioning area is inclined downward from one side to the direction of the containing groove, the bottom of the display strip positioning area is inclined downward from one side to the direction of the containing groove, and the lowest part of the sample adding strip positioning area is lower than that of the display strip positioning area.

[0010] Further, the lowest part of the sample adding strip positioning area is 0.2-2mm lower than the lowest part of the display strip positioning area.

[0011] Further, the lowest part of the sample adding strip positioning area is 1mm lower than the lowest part of the display strip positioning area.

[0012] Further, the display strip comprises chromatography membrane and water absorption pad which are connected in sequence, and the chromatography membrane is located at the side close to the gold label freeze-dried and partially extends out of the display strip positioning area.

[0013] Further, a display strip bottom plate corresponding to the display strip positioning area is further included, and the chromatography membrane and the water absorption pad are connected in sequence on the display strip bottom plate, and the side of the chromatography membrane close to the gold label freeze-dried extends out of the display strip bottom plate.

[0014] Further, the detection window corresponds to the chromatography membrane.

[0015] Further, the sample pad is made of glass cellulose membrane or polyester membrane, and the chromatography membrane is made of non-back support nitrocellulose membrane.

[0016] Further, a sample adding strip bottom plate corresponding to the sample adding strip positioning area is further included, and the sample pad and the gold label freeze-dried are connected in sequence on the sample adding strip bottom plate, and the sample adding strip bottom plate is provided with a groove corresponding to the containing groove.

[0017] Further, the sample adding hole is provided with a funnel-shaped liquid guiding part.

[0018] The beneficial effects of the utility model are as follows: the bottom support and the upper cover are combined to form a cavity for accommodating the test strip, and the test strip is protected. The sample adding strip positioning area on the bottom support is used for placing the sample pad of the test strip, the display strip positioning area is used for placing the display strip, and the containing groove is used for accommodating the sample liquid and the gold label freeze-dried reaction. The sample pad and the gold label freeze-dried are connected to enable the sample liquid of the sample pad to contact the gold label freeze-dried, and the gold label freeze-dried is disconnected from the display strip, so that when the sample liquid passes through the gold label freeze-dried, the gold label freeze-dried is dissolved to make the sample liquid and the gold label freeze-dried flow into the containing groove without directly contacting the display strip. At this time, the detection target in the slowly flowing sample liquid and the antibody in the gold label freeze-dried continuously react to form a binding complex in the containing groove, and the reaction time of the gold label freeze-dried and the sample liquid is improved. The side of the display strip close to the containing groove extends out of the display strip positioning area, so that the liquid level in the containing groove can contact the display strip after rising, and the detection target and the binding complex in the liquid are chromatographed towards the chromatography membrane of the display strip and reach the water absorption pad area.

[0019] In use, when the sample solution is added from the sample hole to the sample pad and then chromatographed towards the gold label freeze-dried direction, when flowing through the gold label freeze-dried, since one side of the gold label freeze-dried is overlapped with the sample pad and the other side is disconnected with the display strip, the gold label freeze-dried is quickly dissolved after contacting with the liquid, and the volume is reduced, and the sample solution and the dissolved gold label freeze-dried are all flowed into the containing groove for reaction, at this time, since the sample solution slowly flows into the containing groove through the sample hole and the sample pad, the liquid level in the containing groove is gradually increased, before the liquid level reaches the display strip, the detection target in the sample solution can continuously react with the antibody, and at this time, the sample solution continuously flows into the containing groove, so that the amount of the detection target in the containing groove is continuously increased, when the liquid level in the containing groove gradually rises to contact with the chromatography membrane, the reacted liquid chromatographs towards the chromatography membrane and reaches the water absorption pad area, at this time, the detection result can be observed through the detection window.

[0020] In summary, the utility model has the advantages of ingenious structure, which can effectively prolong the reaction time of the detection target and the gold label antibody, increase the concentration of the reaction target and the gold label antibody, reduce the influence of the external environment on the detection result, and effectively improve the detection sensitivity and accuracy.

[0021] The utility model will be further described in connection with the drawings and specific embodiments. BRIEF DESCRIPTION OF DRAWINGS

[0022] Fig. 1 The structure diagram of the utility model;

[0023] Fig. 2 The structure diagram of the upper cover of the utility model.

[0024] In the drawings: 1-bottom support, 11-sample adding strip positioning area, 12-containing groove, 13-display strip positioning area, 2-test strip, 21-sample pad, 22-display strip, 221-chromatography membrane, 222-water absorption pad, 23-gold label freeze-dried, 24-display strip bottom plate, 25-sample adding strip bottom plate, 251-groove, 3-upper cover, 31-sample hole, 32-detection window, 33-hopper-shaped liquid guide part. DETAILED DESCRIPTION

[0025] The specific implementation scheme of the utility model will be described in detail with reference to the drawings.

[0026] Reference Figs. 1-2The utility model provides a novel lateral flow assay card, including the bottom support 1, the bottom support 1 is equipped with sample adding strip positioning area 11, containing groove 12 and display strip positioning area 13 in proper order along its length direction, the horizontal height of containing groove 12 is lower than the horizontal height of sample adding strip positioning area 11 and display strip positioning area 13. Further, containing groove 12 is horizontal groove, the bottom of sample adding strip positioning area 11 is inclinedly arranged from one side to the direction of containing groove 12 downward, and sample liquid flows into containing groove 12 from sample pad 21; the bottom of display strip positioning area 13 is inclinedly arranged from one side to the direction of containing groove 12 downward, for slowing down the chromatography speed of target detection object and antibody, and increasing reaction time; the lowest place of sample adding strip positioning area 11 is lower than the lowest place of display strip positioning area 13, so that containing groove 12 can accommodate enough detection target object and antibody after reaction and then chromatography in the direction of display strip 22. Wherein, the lowest place of sample adding strip positioning area 11 is lower than the lowest place of display strip positioning area 13 by 0.2-2mm. For example, the lowest place of sample adding strip positioning area 1111 is lower than the display strip positioning area 13 by 0.5mm, 1mm, 1.5mm; preferably, the lowest place of sample adding strip positioning area 11 is lower than the lowest place of display strip positioning area 13 by 1mm.

[0027] Test strip 2, the test strip 2 includes the sample pad 21 placed on the sample adding strip positioning area 11, wherein, the sample pad 21 is made of glass cellulose membrane or polyester film. Further, it also includes sample adding strip bottom plate 25 corresponding to the sample adding strip positioning area 11, the sample pad 21 and the gold standard freeze-dried 23 are overlapped in sequence on the sample adding strip bottom plate 25, the sample adding strip bottom plate 25 is equipped with recess 251 corresponding to the containing groove 12. In this way, the sample adding strip bottom plate 25 supports the sample pad 21, and when the recess 251 falls into the containing groove 12 when the sample adding strip bottom plate 25 is placed on the sample adding strip positioning area 11, that is, in this way, the recess 251 is used for the reaction of the target detection object of sample liquid and gold standard antibody.

[0028] Test strip 2 also includes display strip 22 placed on the display strip positioning area 13 and gold standard freeze-dried 23 arranged corresponding to the containing groove 12, wherein, the gold standard freeze-dried 23 is made of freeze-drying technology, the display strip 22 includes chromatography membrane 221 and water absorption pad 222 overlapped in sequence, and detection window 32 corresponds to the area of chromatography membrane 221. Preferably, the chromatography membrane 221 is made of no back support nitrocellulose membrane.

[0029] The sample pad 21 and the gold label lyophilized 23 are overlapped in sequence to enable the sample liquid to flow from the sample pad 21 to the gold label lyophilized 23, the other side of the gold label lyophilized 23 is disconnected from the display strip 22, so that when the sample liquid passes through the gold label lyophilized 23, the gold label lyophilized 23 is dissolved to make the sample liquid and the gold label lyophilized 23 flow into the containing groove 12 without directly contacting the display strip 22, at this time, the detection target in the slowly flowing sample liquid and the antibody in the gold label lyophilized 23 continue to react in the containing groove 12 to form a binding complex, thereby increasing the reaction time of the gold label lyophilized 23 and the sample liquid.

[0030] The display strip 22 extends out of the display strip positioning area 13 on the side close to the containing groove 12, further, the chromatography membrane 221 of the display strip 22 is located on the side close to the gold label lyophilized 23 and partially extends out of the display strip positioning area 13, which can enable the liquid level in the containing groove 12 to rise and contact the display strip 22, so that the detection target and the binding complex in the liquid are chromatographed towards the chromatography membrane 221 of the display strip 22 and reach the area of the water absorption pad 222.

[0031] Further, a display strip bottom plate 24 corresponding to the display strip positioning area 13 is further included, the chromatography membrane 221 and the water absorption pad 222 are overlapped in sequence on the display strip bottom plate 24, and the chromatography membrane 221 extends out of the display strip bottom plate 24 on the side close to the gold label lyophilized 23. In this way, the display strip bottom plate 24 supports the chromatography membrane 221 and the water absorption pad 222, and the chromatography membrane 221, the water absorption pad 222 and the display strip bottom plate 24 become a whole, thereby ensuring the structural stability.

[0032] The upper cover 3 is arranged on the bottom support 1 to form a cavity for accommodating the test strip 2 for loading the test strip 2, the upper cover 3 is provided with a sample adding hole 31 corresponding to the sample pad 21 and a detection window 32 corresponding to the display strip 22, the sample adding hole 31 is used to add sample liquid to the sample pad 21, and the detection window 32 is used to observe the detection result, wherein the detection window 32 is isolated from the external environment by a transparent film to prevent environmental factors from causing inaccurate results and facilitate observation of the detection result. Further, the sample adding hole 31 is provided with a funnel-shaped liquid guiding part 33, which can guide the sample liquid and accommodate more sample liquid.

[0033] In use, when the sample solution is added from the sample hole 31 to the sample pad 21 and is chromatographed towards the gold label lyophilized 23, when the gold label lyophilized 23 is contacted with the liquid, the gold label lyophilized 23 is quickly dissolved and the volume is reduced, and the sample solution and the dissolved gold label lyophilized 23 are both flowed into the containing groove 12 to react, at this time, because the sample solution is slowly flowed into the containing groove 12 through the sample hole 31 and the sample pad 21, the liquid level in the containing groove 12 is gradually increased, before the liquid level reaches the display strip 22, the detection target in the sample solution can continuously react with the antibody, and at this time, the sample solution continuously flows into the containing groove 12, so that the amount of the detection target in the containing groove 12 continuously increases, when the liquid level in the containing groove 12 gradually rises to contact the chromatographic membrane 221, the reacted liquid is chromatographed towards the chromatographic membrane 221 and reaches the water absorption pad 222 area, at this time, the detection result can be observed through the detection window 32.

[0034] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, the different embodiments or examples described in the present specification and the features of the different embodiments or examples can be combined and combined by those skilled in the art without contradiction.

[0035] The above only describes the preferred embodiments of the present application and is not intended to limit the present application, although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments or make equivalent replacement for part of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A novel lateral flow test card, characterized in that, The application relates to a test strip for detecting a target substance in a sample, which comprises a base support (1) provided with a sample adding strip positioning area (11), a containing groove (12) and a display strip positioning area (13) in sequence along the length direction of the base support (1), wherein the horizontal height of the containing groove (12) is lower than that of the sample adding strip positioning area (11) and the display strip positioning area (13); a test strip (2) comprising a sample pad (21) arranged on the sample adding strip positioning area (11), a display strip (22) arranged on the display strip positioning area (13) and a gold label freeze-dried product (23) arranged corresponding to the containing groove (12), wherein the sample pad (21) and the gold label freeze-dried product (23) are connected in sequence, the other side of the gold label freeze-dried product (23) is disconnected from the display strip (22), and the side of the display strip (22) close to the containing groove (12) extends out of the display strip positioning area (13); and an upper cover (3) arranged on the base support (1) to form a cavity for containing the test strip (2), wherein the upper cover (3) is provided with a sample adding hole (31) corresponding to the sample pad (21) and a detection window (32) corresponding to the display strip (22). The bottom of the sample adding strip positioning area (11) is inclined downward from one side to the direction of the containing groove (12), and the bottom of the display strip positioning area (13) is also inclined downward from one side to the direction of the containing groove (12), wherein the lowest part of the sample adding strip positioning area (11) is lower than that of the display strip positioning area (13). The lowest part of the sample adding strip positioning area (11) is 0.2-2 mm lower than that of the display strip positioning area (13). The lowest part of the sample adding strip positioning area (11) is 1 mm lower than that of the display strip positioning area (13).

2. The novel lateral flow test card according to claim 1, characterized in that, The display strip (22) comprises a chromatography membrane (221) and a water absorption pad (222) connected in sequence, and the chromatography membrane (221) is located on the side close to the gold label freeze-dried product (23) and partially extends out of the display strip positioning area (13).

3. The novel lateral flow test card according to claim 2, characterized in that, The display strip (22) further comprises a display strip bottom plate (24) corresponding to the display strip positioning area (13), wherein the chromatography membrane (221) and the water absorption pad (222) are connected in sequence on the display strip bottom plate (24), and the side of the chromatography membrane (221) close to the gold label freeze-dried product (23) extends out of the display strip bottom plate (24).

4. The novel lateral flow test card according to claim 3, characterized in that, The detection window (32) corresponds to the chromatography membrane (221).

5. The novel lateral flow test card according to claim 1, characterized in that, The sample pad (21) is made of glass cellulose membrane or polyester membrane, and the chromatography membrane (221) is made of nitrocellulose membrane without back support.

6. The novel lateral flow test card according to claim 5, characterized in that, The display strip (22) further comprises a display strip bottom plate (24) corresponding to the display strip positioning area (13), wherein the chromatography membrane (221) and the water absorption pad (222) are connected in sequence on the display strip bottom plate (24), and the side of the chromatography membrane (221) close to the gold label freeze-dried product (23) extends out of the display strip bottom plate (24).

7. The novel lateral flow test card according to claim 5, characterized in that, The sample adding hole (31) is provided with a funnel-shaped liquid guiding part (33).

8. The novel lateral flow test card according to claim 5, characterized in that, ​ 9. The novel lateral flow test strip according to claim 1, characterized in that, ​ 10. The novel lateral flow test strip according to claim 1, characterized in that, ​

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