Special-shaped diaphragm structure and microscopic lighting device

By employing an irregular aperture structure in the microscope, combining annular and aperture-shaped apertures, complementary central and oblique illumination is achieved, solving the problem of insufficient field brightness for low-contrast transparent samples and improving the imaging speed and efficiency of the microscope.

CN223926706UActive Publication Date: 2026-02-17SHANGHAI JIAOTONG UNIV
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Patent Information

Application Number
CN202520172476.7
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2025-01-24
Publication Date
2026-02-17
Estimated Expiration
2035-01-24

AI Technical Summary

Technical Problem

Existing technologies result in low field-of-view brightness in the microscopic observation of low-contrast transparent samples, making it difficult to meet the rapid imaging requirements of fully automated microscopes, such as high-speed scanning and autofocus.

Method used

An irregular aperture structure is adopted, including a first ring and a second ring, forming an annular aperture and an aperture-shaped aperture. Combined with a condenser lens module, complementary central illumination and oblique illumination are achieved to improve the contrast of the boundary contour of transparent samples.

Benefits of technology

It enhances the illumination intensity of low-contrast transparent samples, improves energy utilization, meets the rapid imaging requirements of fully automated microscopes, and does not require large-scale changes to optical components, facilitating rapid microscope modification.

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Abstract

The utility model provides a special-shaped diaphragm structure and a microscopic lighting device. The special-shaped diaphragm structure comprises a first circular ring and a second circular ring, a second circular ring is arranged on the outer ring side of the first circular ring, and the second circular ring and the first circular ring are concentrically arranged; an annular diaphragm is formed in a light-transmitting area between the second circular ring and the first circular ring, a hole-shaped diaphragm is formed in a light-transmitting area on the inner ring side of the first circular ring, and the hole-shaped diaphragm is located on the inner ring side of the annular diaphragm and is concentric with the annular diaphragm. According to the method, the definition of the boundary contour of the low-contrast transparent sample can be greatly enhanced, compared with phase contrast illumination, the illumination intensity loss is small, the energy utilization rate is high, and the rapid imaging requirements of high-speed scanning, automatic focusing and the like of a full-automatic microscope can be basically met.
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Description

TECHNICAL FIELD

[0001] The utility model relates to the field of microscopic lighting device, specifically, it relates to special-shaped diaphragm structure and microscopic lighting device. BACKGROUND

[0002] At present, the microscopic observation of low contrast transparent sample, such as undyed algae, bacteria, living cell and the like, often adopts the method of phase contrast illumination, and the shortcoming is that the field brightness is low, and the exposure time required is long, so that the high speed scanning, automatic focusing and other rapid imaging requirements of full-automatic microscope are difficult to meet. UTILITY MODEL CONTENT

[0003] In view of the defects in the prior art, the utility model aims at providing a special-shaped diaphragm structure and a microscopic lighting device.

[0004] According to the special-shaped diaphragm structure provided by the utility model, it comprises: a first circular ring and a second circular ring.

[0005] The outer ring side of the first circular ring is provided with the second circular ring, and the second circular ring and the first circular ring are arranged with the same center.

[0006] The light transmission area between the second circular ring and the first circular ring forms an annular diaphragm, the light transmission area of the inner ring side of the first circular ring forms a hole-shaped diaphragm, and the hole-shaped diaphragm is arranged on the inner ring side of the annular diaphragm and with the same center as the annular diaphragm.

[0007] Preferably, the inner ring side of the second circular ring is connected to the outer ring side of the first circular ring through a plurality of thin rod-shaped connecting structures.

[0008] Preferably, the plurality of thin rod-shaped connecting structures divide the light transmission area between the second circular ring and the first circular ring into a plurality of fan-shaped areas with the same shape.

[0009] Preferably, the area ratio of the light transmission area of the hole-shaped diaphragm to the annular diaphragm is 1:100 to 10:1.

[0010] Preferably, a microscopic lighting device adopts the special-shaped diaphragm structure.

[0011] Preferably, it comprises: a special-shaped diaphragm structure and a condenser module.

[0012] The front end of the condenser module towards the sample direction is provided with the special-shaped diaphragm structure.

[0013] Preferably, the outer side of the lens of the condenser module close to the end of the sample direction is provided with the hole-shaped diaphragm and the annular diaphragm of the special-shaped diaphragm structure.

[0014] Preferably, the second annular outer ring of the special-shaped diaphragm structure is connected with a cylindrical shell, and the cylindrical shell is sleeved on the end of the condenser lens module.

[0015] Compared with the prior art, the utility model has the beneficial effects that:

[0016] 1、 The application can greatly enhance the clarity of the low-contrast transparent sample boundary profile, compared with the phase contrast illumination, the illumination intensity loss is smaller, the energy utilization rate is high, and the rapid imaging requirements such as full-automatic microscope high-speed scanning and automatic focusing can be basically met;

[0017] 2、 The utility model only needs to increase a special-shaped diaphragm structure on the basis of traditional illumination, without the need of changing large optical components, and can be suitable for rapid modification of various microscopes. BRIEF DESCRIPTION OF DRAWINGS

[0018] Other features, objects and advantages of the utility model will become more apparent through reading the following detailed description of non-restrictive embodiments with reference to the accompanying drawings:

[0019] Figure 1 It is a structural schematic view of the special-shaped diaphragm structure;

[0020] Figure 2 It is a schematic view of installing the special-shaped diaphragm structure on the condenser lens module;

[0021] Figure 3 It is a principle view of the special-shaped diaphragm structure;

[0022] The drawings show:

[0023] DETAILED DESCRIPTION

[0024] The utility model will be described in detail in combination with specific embodiments. The following embodiments will help the person skilled in the art to further understand the utility model, but do not limit the utility model in any form. It should be pointed out that, for ordinary skilled in the art, without departing from the concept of the utility model, a number of changes and improvements can be made. These all belong to the protection scope of the utility model.

[0025] In the embodiment, the special-shaped diaphragm structure 1 combined with the concentric structure of the hole diaphragm 11 and the ring diaphragm 12 is adopted on the condenser lens module 2 of the microscope, the center illumination and the oblique illumination are complementary, the uniformity and the intensity of the illumination are ensured through the center illumination, and the contrast of the transparent sample boundary profile is improved through the oblique illumination using the change of different medium refraction and scattering under the transparent sample.

[0026] Specifically, as Figure 1As shown, this embodiment includes: a first ring and a second ring; the second ring is disposed on the outer ring side of the first ring, and the second ring and the first ring are concentrically arranged; the inner ring side of the second ring is connected to the outer ring side of the first ring through multiple thin rod-shaped connecting structures. The multiple thin rod-shaped connecting structures divide the light-transmitting area between the second ring and the first ring into multiple fan-shaped areas of the same shape. The light-transmitting area between the second ring and the first ring forms an annular aperture 12, and the light-transmitting area on the inner ring side of the first ring forms an aperture 11, which is located on the inner ring side of the annular aperture 12 and is concentrically arranged with the annular aperture 12.

[0027] In one embodiment, the ratio of the light-transmitting area of ​​the aperture 11 to the annular aperture 12 is 1:100 to 10:1, which means that the contribution of the aperture 11 and the annular aperture 12 to the sample illumination intensity is between 1:20 and 20:1.

[0028] like Figure 2 As shown, a microscopic illumination device with the irregular aperture structure 1 of this embodiment is installed, including: the irregular aperture structure 1 and the condenser lens module 2; the irregular aperture structure 1 is provided at the front end of the condenser lens module 2 facing the sample direction. A cylindrical housing is connected to the outer ring side of the second ring of the irregular aperture structure 1, and the cylindrical housing is fitted onto the end of the condenser lens module 2.

[0029] In one implementation, such as Figure 3 As shown, the outer side of the lens of the condenser module 2 near the sample direction is provided with an aperture-shaped aperture 11 and an annular aperture 12 of the irregular aperture structure 1. That is, the irregular aperture structure 1 is placed between the condenser module 2 and the sample, and is close to the position of the last lens of the condenser module 2.

[0030] In one embodiment, the cylindrical housing of the irregular aperture structure 1 can be connected to the outer shell of one end of the condenser module 2 by thread, or it can be installed by interference fit, adhesive bonding or other methods.

[0031] In one embodiment, the cylindrical shell of the irregular aperture structure 1 has various diameters to accommodate condenser lens modules 2 of different sizes.

[0032] In the description of this application, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0033] The specific embodiments of the present application are described above. It needs to be understood that the present application is not limited to the specific embodiments described above, and various changes or modifications can be made by those skilled in the art within the scope of the claims, which does not affect the essential content of the present application. In the case of no conflict, the embodiments of the present application and the features in the embodiments can be combined with each other arbitrarily.

Claims

1. A heteromorphic diaphragm structure, characterized by, Comprising: a first circular ring and a second circular ring; the outer ring side of the first circular ring is provided with the second circular ring, and the second circular ring and the first circular ring are coaxial; the light transmission area between the second circular ring and the first circular ring forms an annular diaphragm (12), and the light transmission area on the inner ring side of the first circular ring forms a hole-shaped diaphragm (11), which is located on the inner ring side of the annular diaphragm (12) and is coaxial with the annular diaphragm (12).

2. The anamorphic diaphragm structure of claim 1, wherein: The inner ring side of the second circular ring is connected to the outer ring side of the first circular ring through a plurality of thin rod-shaped connecting structures.

3. The anamorphic diaphragm structure of claim 2, wherein: The plurality of thin rod-shaped connecting structures divide the light transmission area between the second circular ring and the first circular ring into a plurality of fan-shaped areas of the same shape.

4. The anamorphic diaphragm structure of claim 1, wherein: The area ratio of the hole-shaped diaphragm (11) to the light transmission area of the annular diaphragm (12) is 1:100 to 10:

1.

5. A microscope illumination device, characterized by: The special-shaped diaphragm structure of any one of claims 1-4 is adopted.

6. The microscope illumination device of claim 5, wherein, Comprising: a special-shaped diaphragm structure (1) and a condenser module (2); the front end of the condenser module (2) in the sample direction is provided with the special-shaped diaphragm structure (1).

7. The microscope illumination device of claim 6, wherein: The outer side of the lens near the end of the condenser module (2) in the sample direction is provided with the hole-shaped diaphragm (11) and the annular diaphragm (12) of the special-shaped diaphragm structure (1).

8. The microscope illumination device of claim 6, wherein: The outer ring side of the second circular ring of the special-shaped diaphragm structure (1) is connected with a cylindrical shell, and the cylindrical shell is sleeved on the end of the condenser module (2).