Liquid level control device for perfusion culture

Abstract

The utility model belongs to the technical field of perfusion culture, and particularly relates to a liquid level control device for perfusion culture, which comprises a bottom plate, a floating plate and a laser range finder, supporting legs are fixedly installed at the bottom of the bottom plate, a fixing support is fixedly installed at the top of the bottom plate, a tank body is fixedly installed on the inner wall of the fixing support, a liquid inlet assembly is arranged at the top of the tank body, a liquid outlet assembly is arranged at the bottom of the bottom plate, and a supporting assembly is arranged on the inner wall of the tank body. A measuring assembly is arranged on the inner wall of the tank body; through the arrangement of a liquid inlet pump, a first liquid conveying pipe, a liquid outlet pump and a second liquid conveying pipe, the liquid inlet pump and the first liquid conveying pipe are matched for use, the liquid inlet amount can be accurately controlled, the height of liquid in the tank body can be effectively controlled, and the liquid outlet pump and the second liquid conveying pipe are matched for use, so that the water outlet amount of the liquid in the tank body can be accurately controlled; and the water level can be accurately controlled by matching with the liquid inlet pump.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of perfusion culture, and specifically relates to a liquid level control device for perfusion culture. BACKGROUND

[0002] In the field of modern biological engineering, perfusion culture, as an advanced cell culture technology, is playing an increasingly important role. By continuously adding fresh medium to the culture system and simultaneously removing old medium containing metabolic waste and cell products, perfusion culture can provide a more stable and suitable growth environment for cells, effectively improving cell density and product yield.

[0003] However, during perfusion culture, maintaining the precise stability of the liquid level of the culture system is one of the key factors to ensure the smooth progress of the culture process. Fluctuations in the liquid level can have multiple adverse effects on cell growth. For example, too low a liquid level can expose cells to air, causing cell damage or even death; too high a liquid level can change the hydrodynamic environment of the culture system, affecting material transfer and gas exchange of cells, and thus interfering with the normal metabolic activity of cells.

[0004] Traditional liquid level control methods have many limitations. Early simple manual monitoring and manual adjustment methods not only consume a lot of manpower and time, but also due to the instability of human operation, it is difficult to achieve precise control of the liquid level, and it is easy to cause large fluctuations in the liquid level, which seriously affects the culture effect. Some liquid level control methods based on simple mechanical devices, although to some extent, realize automation, but the control precision is limited, and it cannot meet the high requirements of modern perfusion culture process for liquid level stability.

[0005] Therefore, the utility model provides a liquid level control device for perfusion culture. UTILITY MODEL CONTENT

[0006] In order to make up for the deficiencies of the prior art and solve at least one problem raised in the background art, a liquid level control device for perfusion culture is proposed.

[0007] The utility model solves its technical problems and adopts the technical scheme: the utility model discloses a liquid level control device for perfusion culture, including bottom plate, floating plate and laser range finder, the bottom of bottom plate is fixedly installed with support leg, the top of bottom plate is fixedly installed with fixed support, the inner wall of fixed support is fixedly installed with jar, the top of jar is provided with liquid inlet assembly, the bottom of bottom plate is provided with liquid outlet assembly, the inner wall of jar is provided with support assembly, the inner wall of jar is provided with measuring assembly.

[0008] Preferably, the liquid inlet assembly comprises a liquid inlet pump and a first liquid inlet pipe, the liquid inlet pump is fixedly installed on the top of the tank body, the output end of the liquid inlet pump is fixedly installed with the tank body, and the input end of the liquid inlet pump is fixedly installed with the first liquid inlet pipe.

[0009] Preferably, the liquid outlet assembly comprises a liquid outlet pump and a second liquid outlet pipe, the liquid outlet pump is fixedly installed on the bottom of the tank body, the input end of the liquid outlet pump is fixedly installed with the tank body, and the output end of the liquid outlet pump is fixedly installed with the second liquid outlet pipe, the cooperation of the liquid outlet pump and the second liquid outlet pipe can accurately control the liquid outlet amount of the liquid in the tank body, and the cooperation with the liquid inlet pump can realize accurate control of the water level.

[0010] Preferably, the support assembly comprises a fixed vertical rod and a support beam, the fixed vertical rod is fixedly installed on the top and bottom of the inner wall of the tank body, and the inner side of the fixed vertical rod is fixedly installed with the support beam, the cooperation of the fixed vertical rod and the support beam can provide stable support for the fixed thin rod, and can also limit the floating ball.

[0011] Preferably, the measuring assembly comprises a fixed thin rod and a floating ball, the fixed thin rod is fixedly installed on the inner wall of the support beam, and the surface of the fixed thin rod is slidably installed with the floating ball, the cooperation of the fixed thin rod and the floating ball can lift according to the buoyancy of the liquid, so as to feedback the height of the liquid.

[0012] Preferably, the floating plate is provided with two groups, the two groups of floating plates are fixedly installed on the top and bottom of the floating ball, and the floating plate is slidably installed with the fixed thin rod, the floating plate can improve the buoyancy of the floating ball and make the measurement more accurate, and the floating plate can also provide a good irradiation environment for the laser range finder.

[0013] Preferably, the laser range finder is provided with two groups, the two groups of laser range finders are fixedly installed on the top and bottom of the inner wall of the support beam, the laser range finder can accurately measure the water level according to the floating height of the floating plate, and feedback the data to the user, so that the user can operate the liquid inlet pump and the liquid outlet pump to adjust the water level.

[0014] Preferably, the surface of the floating ball and the floating plate is coated with anticorrosive paint, the coating of the anticorrosive paint can prevent the floating ball and the floating plate from being corroded after long-term immersion, so that they can be used for a long time.

[0015] The beneficial effects of the utility model are as follows:

[0016] 1. The liquid level control device for perfusion culture, through the setting of the liquid inlet pump, the first infusion tube, the liquid outlet pump and the second infusion tube, the cooperation of the liquid inlet pump and the first infusion tube can accurately control the liquid inlet amount, so that the height of the liquid in the tank body can be effectively controlled, the cooperation of the liquid outlet pump and the second infusion tube can accurately control the water outlet amount of the liquid in the tank body, and the cooperation with the liquid inlet pump can realize accurate control of the water level.

[0017] 2. The liquid level control device for perfusion culture, through the setting of the fixed vertical rod, the supporting cross beam, the fixed thin rod and the floating ball, the cooperation of the fixed vertical rod and the supporting cross beam can provide stable support for the fixed thin rod, and can also limit the floating ball, the cooperation of the fixed thin rod and the floating ball can lift according to the buoyancy of the liquid, so that the height of the liquid can be fed back. BRIEF DESCRIPTION OF DRAWINGS

[0018] The utility model makes further explanation in combination with the drawings.

[0019] Figure 1 It is the front view of the utility model;

[0020] Figure 2 It is the bottom view in the utility model;

[0021] Figure 3 It is the sectional view in the utility model;

[0022] Figure 4 It is Figure 1 The local enlarged view of A in it;

[0023] Figure 5 It is Figure 2 The local enlarged view of B in it;

[0024] Figure 6 It is Figure 3 The local enlarged view of C in it.

[0025] Legend:

[0026] 1, bottom plate; 2, support leg; 3, fixed support; 4, tank body; 5, liquid inlet assembly; 51, liquid inlet pump; 52, first infusion tube; 6, liquid outlet assembly; 61, liquid outlet pump; 62, second infusion tube; 7, support assembly; 71, fixed vertical rod; 72, supporting cross beam; 8, measuring assembly; 81, fixed thin rod; 82, floating ball; 90, floating plate; 100, laser range finder. DETAILED DESCRIPTION

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0028] Specific implementation examples are given below.

[0029] like Figures 1 to 6 As shown in the embodiment of this utility model, a liquid level control device for perfusion culture includes a base plate 1, a float plate 90, and a laser rangefinder 100. A support leg 2 is fixedly installed at the bottom of the base plate 1, a fixed bracket 3 is fixedly installed at the top of the base plate 1, a tank 4 is fixedly installed on the inner wall of the fixed bracket 3, a liquid inlet assembly 5 is provided at the top of the tank 4, a liquid outlet assembly 6 is provided at the bottom of the base plate 1, a support assembly 7 is provided on the inner wall of the tank 4, and a measuring assembly is provided on the inner wall of the tank 4. 8; The liquid inlet assembly 5 includes a liquid inlet pump 51 and a first inlet pipe 52. The liquid inlet pump 51 is fixedly installed on the top of the tank body 4. The output end of the liquid inlet pump 51 is fixedly installed to the tank body 4, and the input end of the liquid inlet pump 51 is fixedly installed to the first inlet pipe 52. The liquid dispensing assembly 6 includes a liquid dispensing pump 61 and a second infusion pipe 62. The liquid dispensing pump 61 is fixedly installed at the bottom of the tank body 4. The input end of the liquid dispensing pump 61 is fixedly installed to the tank body 4, and the output end of the liquid dispensing pump 61 is fixedly installed to the second infusion pipe 62. The support assembly 7 includes a fixed upright 71 and a support beam 72. The fixed upright 71 is fixedly installed at the top and bottom of the inner wall of the tank body 4, and the inner side of the fixed upright 71 is fixedly installed to the support beam 72. The measuring assembly 8 includes a fixed thin rod 81. The float 82 and the fixed thin rod 81 are fixedly installed on the inner wall of the supporting beam 72. The surface of the fixed thin rod 81 is slidably installed with the float 82. Two sets of float plates 90 are provided. The two sets of float plates 90 are fixedly installed on the top and bottom of the float 82. The float plates 90 are slidably installed with the fixed thin rod 81. Two sets of laser rangefinders 100 are provided. The two sets of laser rangefinders 100 are fixedly installed on the top and bottom of the inner wall of the supporting beam 72. The surfaces of the float 82 and the float plates 90 are coated with anti-corrosion paint.

[0030] like Figures 1 to 6As shown, the cooperation of the liquid inlet pump 51 and the first liquid inlet pipe 52 can accurately control the liquid inlet amount, so that the liquid height in the tank body 4 can be effectively controlled, the cooperation of the liquid outlet pump 61 and the second liquid outlet pipe 62 can accurately control the liquid outlet amount of the tank body 4, the cooperation of the liquid inlet pump 51 can realize accurate control of the water level, the cooperation of the fixed vertical rod 71 and the supporting cross beam 72 can provide stable support for the fixed thin rod 81, and the floating ball 82 can be limited, the cooperation of the fixed thin rod 81 and the floating ball 82 can be lifted according to the buoyancy of the liquid, so that the height of the liquid can be fed back, the floating plate 90 can improve the buoyancy of the floating ball 82 to make the measurement more accurate, and the floating plate 90 can also provide a good irradiation environment for the laser range finder 100, the laser range finder 100 can accurately measure the water level according to the floating height of the floating plate 90, and feed back the data to the user, so that the user can operate the liquid inlet pump 51 and the liquid outlet pump 61 to adjust the water level, and the application of the anticorrosive coating can prevent the floating ball 82 and the floating plate 90 from being corroded after being soaked for a long time, so that they can be used for a long time.

[0031] Working principle: when working, first place the bottom plate 1 on a flat position, then connect the first liquid inlet pipe 52 and the second liquid outlet pipe 62 to the liquid inlet and the liquid outlet respectively, then start the liquid inlet pump 51 to make the liquid in the first liquid inlet pipe 52 enter the inside of the tank body 4, when the liquid enters the inside of the tank body 4, the floating ball 82 and the floating plate 90 will slide on the surface of the fixed thin rod 81 due to the buoyancy, the floating plate 90 will start the laser range finder 100 to detect the height of the floating plate 90 when sliding, and feed back to the liquid inlet pump 51 and the liquid outlet pump 61, when the liquid in the tank body 4 is too high, the liquid inlet pump 51 will slow down the feeding speed, and the liquid outlet pump 61 will speed up the discharging speed, so that the liquid in the tank body 4 can be effectively controlled, otherwise the liquid height will be increased, the user only needs to accurately control the liquid inlet pump 51 and the liquid outlet pump 61 to realize accurate control of the water level, the utility model realizes accurate control of the water level through the liquid inlet pump 51 and the liquid outlet pump 61, has the advantages of low production cost, simple structure, convenient maintenance and the like.

[0032] The basic principle, main features and advantages of the utility model are shown and described above. Those skilled in the art should understand that the utility model is not limited by the above examples, and the above examples and descriptions in the specification are only to illustrate the principle of the utility model, and various changes and improvements can be made to the utility model without departing from the spirit and scope of the utility model, and these changes and improvements all fall within the scope of the utility model claimed. The scope of protection of the utility model is defined by the appended claims and their equivalents.

Claims

1. A liquid level control device for perfusion culture, comprising a base plate (1), a floating plate (90) and a laser range finder (100); characterized in that: The bottom of the bottom plate (1) is fixedly installed with supporting legs (2), the top of the bottom plate (1) is fixedly installed with a fixed support (3), the inner wall of the fixed support (3) is fixedly installed with a tank body (4), the top of the tank body (4) is provided with a liquid inlet assembly (5), the bottom of the bottom plate (1) is provided with a liquid outlet assembly (6), the inner wall of the tank body (4) is provided with a supporting assembly (7), and the inner wall of the tank body (4) is provided with a measuring assembly (8).

2. A liquid level control device for perfusion culture according to claim 1, characterized in that: The liquid inlet assembly (5) comprises a liquid inlet pump (51) and a first liquid inlet pipe (52), the liquid inlet pump (51) is fixedly installed at the top of the tank body (4), the output end of the liquid inlet pump (51) is fixedly installed with the tank body (4), and the input end of the liquid inlet pump (51) is fixedly installed with the first liquid inlet pipe (52).

3. A liquid level control device for perfusion culture according to claim 2, wherein: The liquid outlet assembly (6) comprises a liquid outlet pump (61) and a second liquid outlet pipe (62), the liquid outlet pump (61) is fixedly installed at the bottom of the tank body (4), the input end of the liquid outlet pump (61) is fixedly installed with the tank body (4), and the output end of the liquid outlet pump (61) is fixedly installed with the second liquid outlet pipe (62).

4. A liquid level control device for perfusion culture according to claim 3, wherein: The supporting assembly (7) comprises a fixed vertical rod (71) and a supporting cross beam (72), the fixed vertical rod (71) is fixedly installed at the top and bottom of the inner wall of the tank body (4), and the inner side of the fixed vertical rod (71) is fixedly installed with the supporting cross beam (72).

5. A liquid level control device for perfusion culture according to claim 4, wherein: The measuring assembly (8) comprises a fixed thin rod (81) and a floating ball (82), the fixed thin rod (81) is fixedly installed on the inner wall of the supporting cross beam (72), and the surface of the fixed thin rod (81) is slidably installed with the floating ball (82).

6. A liquid level control device for perfusion culture according to claim 5, wherein: The floating plate (90) is provided with two groups, the two groups of floating plates (90) are fixedly installed at the top and bottom of the floating ball (82), and the floating plate (90) is slidably installed with the fixed thin rod (81).

7. A liquid level control device for perfusion culture according to claim 6, wherein: The laser range finder (100) is provided with two groups, and the two groups of laser range finders (100) are fixedly installed at the top and bottom of the inner wall of the supporting cross beam (72).

8. A liquid level control device for perfusion culture according to claim 7, wherein: The surface of the floating ball (82) and the floating plate (90) is coated with anticorrosive paint.

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