Circulating laboratory microalgae rapid breeding device

Abstract

The utility model discloses a circulating type rapid culture device for microalgae in a laboratory, and belongs to the technical field of algae culture. According to the technical scheme, the circulating type laboratory microalgae rapid culture device comprises a culture tank, an aeration unit, a water quality monitoring unit and a controller, the bottom of the culture tank is of an inverted circular truncated cone structure, a top cover is detachably arranged at the top of the culture tank, and a bottom cover is detachably arranged at the bottom of the culture tank; a wave making pump and a heater are arranged in the culture tank; the top cover is provided with a water inlet and an air outlet, the water inlet is connected with a water inlet pipe, and the culture tank is provided with a liquid level sensor; the aeration unit comprises four groups of aeration pipes, the four groups of aeration pipes are communicated with one another through a central pipe and are vertically arranged in the culture tank in a cross shape, a plurality of circular vent holes are uniformly distributed in the outer walls of the four groups of aeration pipes, and an LED lamp tube is arranged in each group of aeration pipes. According to the utility model, the microalgae growth cycle can be shortened, and the microalgae yield is improved.

Description

Technical Field

[0001] This utility model belongs to the field of algae cultivation technology, specifically relating to a recirculating laboratory microalgae rapid cultivation device. Background Technology

[0002] Algae are an important group of eukaryotes in the protist kingdom. They primarily live in water, lack vascular bundles, yet possess the ability to photosynthesize, making them crucial to ecosystems. Long-term microalgae cultivation in laboratories often involves a combination of air pumps and conical flasks, resulting in low yields, slow growth cycles, and inefficient utilization of water and nutrients. Due to the relatively enclosed nature of the cultivation environment and uncontrollable factors during operation, microalgae are easily contaminated by bacteria, fungi, and other microorganisms. These microorganisms compete with microalgae for nutrients and may even produce harmful substances, severely impacting normal growth and quality. Contamination not only reduces microalgae yield but also increases the cost and difficulty of subsequent processing. Sedimentation and adhesion are also significant issues. Due to gravity, some microalgae settle to the bottom of the cultivation device and adhere to the walls, reducing the contact area with light and nutrients, lowering photosynthetic efficiency, and making these adhered algae difficult to extract and utilize effectively. Sedimentation and adhesion not only affect the uniform growth of microalgae but also reduce overall cultivation efficiency. Utility Model Content

[0003] This invention provides a cyclic laboratory microalgae rapid cultivation device that can shorten the microalgae growth cycle and increase microalgae yield.

[0004] The technical solution of this utility model is as follows:

[0005] This is a circulating laboratory microalgae rapid cultivation device, comprising a culture tank, an aeration unit, a water quality monitoring unit, and a controller. The culture tank has an inverted frustum-shaped bottom and a detachable top cover. An outlet is located at the bottom of the tank and is detachably connected to a bottom cover. A filter membrane is installed at the top of the tank to prevent external bacteria from entering when gas is introduced. A wavemaker pump and heater are installed inside the tank to circulate the culture medium. The continuous flow of the culture medium within the tank and circulation system prevents microalgae from settling, ensuring uniform distribution and sufficient contact with light and nutrients. The top cover has an inlet and a vent. The inlet connects to an inlet pipe. A level sensor is installed in the tank. The controller is electrically connected; the aeration unit includes four sets of aeration pipes, which are interconnected through a central pipe and arranged vertically in a cross shape within the culture tank. Several circular air vents are evenly distributed on the outer wall of each of the four aeration pipes, and each aeration pipe contains an LED light tube. The top cover is equipped with a vent pipe, which includes an air inlet valve and a gas flow meter. One end of the vent pipe is connected to an air pump, and the other end is connected to the aeration pipe. Both the air inlet valve and the gas flow meter are electrically connected to the controller. The water quality monitoring unit includes a temperature sensor, a dissolved oxygen sensor, a pH sensor, and a turbidity sensor, all installed within the culture tank. These sensors are electrically connected to the controller and are used to monitor the culture medium data.

[0006] Preferably, the culture tank and aeration pipe are made of acrylic material, the filter membrane is made of PTFE material, and the pore size of the filter membrane is 0.2-0.4 micrometers.

[0007] Preferably, the bottom cover and the culture tank are connected by threads, and a handle is provided at the bottom of the bottom cover.

[0008] Preferably, the diameter of the vent hole is less than half the inner diameter of the aeration pipe.

[0009] Preferably, the end of the vent pipe away from the aeration pipe is connected to an anti-backflow bottle via a branch pipe. The anti-backflow bottle can prevent backflow of the culture medium when the supply of carbon dioxide / oxygen is stopped.

[0010] Preferably, the aeration pipe is further provided with an aeration head, which is spaced apart from the air vents and evenly distributed on the aeration pipe, and the aeration head is provided with an aeration vent.

[0011] Preferably, the aeration head and the aeration pipe are connected by a connecting pipe.

[0012] Compared with the prior art, this utility model has the following advantages:

[0013] 1. The bottom of the culture tank of this utility model adopts a semi-circular structure. Combined with the use of wave pump and aeration unit, it prevents microalgae from settling and sticking to the wall, improves the contact efficiency of microalgae with light and nutrients, and promotes the rapid growth of microalgae.

[0014] 2. This utility model integrates an aeration unit, a water quality monitoring unit, and a controller to achieve real-time monitoring and precise control of key parameters such as light (via LED lamps), temperature, dissolved oxygen, pH value, and turbidity, providing an optimal growth environment for microalgae.

[0015] 3. The top and bottom of the culture tank are removable, making it easy to change the culture medium and perform equipment maintenance; at the same time, the threaded bottom cover and the handle design further simplify the operation process.

[0016] 4. The aeration pipe is set vertically in a cross shape, with evenly distributed air holes and aeration heads, which realizes uniform aeration in the aquaculture liquid and improves dissolved oxygen efficiency; at the same time, the stability and adjustability of the aeration volume are ensured by the precise control of the gas flow meter and the air inlet valve.

[0017] 5. The anti-backflow bottle design, with the venting pipe connected to the branch pipe, effectively prevents the backflow of the culture medium when the supply of carbon dioxide / oxygen is stopped, ensuring the safety and stability of the aquaculture device. Attached Figure Description

[0018] Figure 1 This is the main view structural diagram of this utility model.

[0019] Figure 2 This is a side view of the structure of this utility model.

[0020] Figure 3 This is a top view of the structure of this utility model.

[0021] Figure 4 This is a schematic diagram of the structure of the aeration pipe of this utility model.

[0022] In the diagram, 1. Culture tank; 2. Top cover; 3. Bottom cover; 4. Inlet; 5. Vent; 6. Outlet; 7. Aeration pipe; 8. Vent hole; 9. LED tube; 10. Vent pipe; 11. Temperature sensor; 12. Dissolved oxygen sensor; 13. pH sensor; 14. Turbidity sensor; 15. Handle; 16. Anti-backflow bottle; 17. Aeration head; 18. Liquid level sensor. Detailed Implementation

[0023] To enable those skilled in the art to better understand the technical solutions of this utility model, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of this utility model.

[0024] Example 1

[0025] like Figure 1-4 As shown, this embodiment provides a circulating laboratory microalgae rapid cultivation device, including a cultivation tank 1, an aeration unit, a water quality monitoring unit, and a controller. The cultivation tank 1 and the aeration pipe 7 are both made of acrylic material. The transparent material can better accept natural light. Combined with artificial light sources, it reduces the impact of environmental factors on the device and maximizes photosynthetic efficiency. The bottom of the cultivation tank 1 is an inverted frustum structure, which facilitates the sedimentation and outflow of microalgae. The top of the cultivation tank 1 is detachably equipped with a top cover 2. The bottom of the cultivation tank 1 is equipped with a water outlet 6. The water outlet 6 is detachably connected to a bottom cover 3. The bottom cover 3 is threadedly connected to the cultivation tank 1. The bottom of the bottom cover 3 is equipped with a handle 15.

[0026] The top of the culture tank 1 is equipped with a PTFE filter membrane with a pore size of 0.2 micrometers. A wavemaker pump and a heater are installed inside the culture tank 1. The wavemaker pump circulates the culture medium within the device, ensuring continuous flow and preventing microalgae sedimentation. This ensures uniform distribution and adequate contact with light and nutrients. The top cover 2 has a water inlet 4 and a vent 5. The vent 5 releases excess gas from the device. The water inlet 4 passes through the filter membrane and connects to the water inlet pipe. The culture tank 1 is equipped with a liquid level sensor 18. The liquid level sensor 18 is electrically connected to the controller; the aeration unit includes four sets of aeration pipes 7, which are interconnected through a central pipe and are vertically arranged in a cross shape in the culture tank 1. Several circular vent holes 8 are evenly distributed on the outer wall of the four sets of aeration pipes 7. The diameter of the vent holes 8 is less than half the inner diameter of the aeration pipe 7. The aeration pipe 7 is also provided with an aeration head 17, which is spaced apart from the vent holes 8 and evenly distributed on the aeration pipe 7. The aeration head 17 is provided with an aeration hole, and the aeration head 17 is connected to the aeration pipe 7 through a connecting pipe.

[0027] Each aeration pipe 7 is equipped with an LED light tube 9; the top cover 2 is equipped with a vent pipe 10, which is equipped with an air inlet valve and a gas flow meter. One end of the vent pipe 10 is connected to an air pump, and the other end is connected to the aeration pipe 7 through a central pipe. The air inlet valve and the gas flow meter are both electrically connected to the controller; the end of the vent pipe 10 away from the aeration pipe 7 is connected to an anti-backflow bottle 16 through a branch pipe; the water quality detection unit includes a temperature sensor, a dissolved oxygen sensor, a pH sensor, and a turbidity sensor installed in the culture tank 1. The temperature sensor, dissolved oxygen sensor, pH sensor, and turbidity sensor are all electrically connected to the controller. The water quality detection unit is used to monitor the culture medium data. The temperature sensor is a BRW600-400A temperature sensor provided by the Intelligent Manufacturing Industrial Park of Yuhua Economic Development Zone, Changsha City, Hunan Province; the dissolved oxygen sensor is a MIK-D07017 dissolved oxygen sensor provided by Hangzhou Meikong Automation Technology Co., Ltd.; the pH sensor is a SIN-pH10RP6.8 pH sensor provided by Hangzhou Liance Automation Technology Co., Ltd.; and the turbidity sensor is a SIN-PTU-8018 turbidity sensor provided by Hangzhou Liance Automation Technology Co., Ltd.

[0028] Working Process: The culture medium enters the culture tank 1 through inlet 4. The water level is monitored in real time by level sensor 18 and connected to the controller to maintain an appropriate water level. The wave generator pump starts, and the air pump supplies air to the aeration pipe 7 through the air pipe 10. The air vents 8 and aeration heads 17 on the aeration pipe 7 release air bubbles evenly, propelling the culture medium to flow continuously in the culture tank 1 and the circulation system, preventing microalgae sedimentation and ensuring that the microalgae are evenly distributed and fully exposed to light and nutrients. The LED tubes 9 inside the aeration pipe 7 provide additional light source, and the light intensity and spectrum are adjusted according to the controller's instructions to optimize microalgae growth conditions. The air inlet valve and gas flow meter control the gas flow rate and are connected to the controller to ensure stable aeration. The temperature sensor, dissolved oxygen sensor, pH sensor, and turbidity sensor in the water quality detection unit monitor the water quality parameters of the culture medium in real time. The sensor data is transmitted to the controller, which automatically adjusts the aeration rate, light intensity, water inlet and outlet, and other operations. When the temperature is too low, the heater is turned on to heat the nutrient solution. The pH can be adjusted by adding acid or alkali to maintain the water quality within a suitable range. After the microalgae cultivation cycle is completed, the aeration unit and wave generator in the device can be turned off. The anti-backflow bottle 16 prevents gas backflow and protects the air pump. The microalgae are allowed to settle to the bottom of the cultivation tank 1, and the outlet 6 is opened to collect only the settled microalgae. After collection, the water level drops. The water level is monitored by a water level detector, and the culture medium is replenished again for the next cultivation cycle. This reduces water and nutrient waste and improves resource utilization.

[0029] Although the present invention has been described in detail with reference to the accompanying drawings and preferred embodiments, it is not limited thereto. Various equivalent modifications or substitutions can be made to the embodiments of the present invention by those skilled in the art without departing from the spirit and essence of the present invention, and all such modifications or substitutions should be within the scope of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A circulating laboratory microalgae rapid cultivation device, characterized in that, The system includes a culture tank (1), an aeration unit, a water quality monitoring unit, and a controller. The top of the culture tank (1) is detachably equipped with a top cover (2). The bottom of the culture tank (1) is an inverted frustum structure. The bottom of the culture tank (1) is equipped with a water outlet (6), which is detachably connected to a bottom cover (3). A filter membrane is installed at the top of the culture tank (1). A wave generator and a heater are installed inside the culture tank (1). The top cover (2) is provided with a water inlet (4) and a vent (5). The water inlet (4) is connected to the water inlet pipe. The culture tank (1) is provided with a liquid level sensor (18). The liquid level sensor (18) is electrically connected to the controller. The aeration unit includes four sets of aeration pipes (7). The four sets of aeration pipes (7) are interconnected through a central pipe and are vertically arranged in a cross shape in the culture tank (1). Several circular ventilation holes (8) are evenly distributed on the outer wall of the four sets of aeration pipes (7). Each set of aeration pipes (7) is equipped with an LED lamp tube (9). The top cover (2) is equipped with a vent pipe (10), and the vent pipe (10) is equipped with an air inlet valve and a gas flow meter. One end of the vent pipe (10) is connected to the air pump, and the other end is connected to the aeration pipe (7). The air inlet valve and the gas flow meter are both electrically connected to the controller. The water quality detection unit includes a temperature sensor (11), a dissolved oxygen sensor (12), a pH sensor (13), and a turbidity sensor (14) installed in the culture tank (1). The temperature sensor (11), dissolved oxygen sensor (12), pH sensor (13), and turbidity sensor (14) are all electrically connected to the controller.

2. The circulating laboratory microalgae rapid cultivation device as described in claim 1, characterized in that, Both the culture tank (1) and the aeration pipe (7) are made of acrylic.

3. The circulating laboratory microalgae rapid cultivation device as described in claim 1, characterized in that, The bottom cover (3) is connected to the culture tank (1) by a thread, and a handle (15) is provided at the bottom of the bottom cover (3).

4. The circulating laboratory microalgae rapid cultivation device as described in claim 1, characterized in that, The diameter of the vent (8) is less than half the inner diameter of the aeration pipe (7).

5. The circulating laboratory microalgae rapid cultivation device as described in claim 1, characterized in that, The end of the vent pipe (10) away from the aeration pipe (7) is connected to an anti-backflow bottle (16) via a branch pipe.

6. The circulating laboratory microalgae rapid cultivation device as described in claim 1, characterized in that, The aeration pipe (7) is also provided with an aeration head (17). The aeration head (17) and the air vent (8) are spaced apart and evenly distributed on the aeration pipe (7). The aeration head (17) is provided with an aeration vent.

7. The circulating laboratory microalgae rapid cultivation device as described in claim 6, characterized in that, The aeration head (17) and the aeration pipe (7) are connected by a connecting pipe.

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