Fluorescence detection device based on quantum dots

Abstract

The utility model relates to the technical field of biological detection, in particular to a fluorescence detection device based on quantum dots. The fluorescence detection device based on the quantum dots comprises a workbench, a side plate and a stand column, the side plate and the stand column are fixedly installed on the upper surface of the workbench, a fluorescence detector is fixedly installed on the side plate, two sets of ring frames are installed on the stand column, integrating spheres are fixedly installed on the two sets of ring frames, a light inlet pipe is installed at the top ends of the integrating spheres, and a light outlet pipe is installed at the top end of the light inlet pipe. A laser generator is installed at the top end of the light inlet pipe, a detection pipe is communicated with the side, close to the side plate, of the integrating sphere, the detection pipe is communicated with the detection end of the fluorescence detector through a guide pipe, a sample injection blind pipe is installed at the bottom end of the integrating sphere, and a sample injection groove is formed in the side, facing the stand column, of the sample injection blind pipe; and an automatic sample injection assembly matched with the sample injection groove is mounted on the stand column. The fluorescence detection device based on quantum dots provided by the utility model has the advantages of high detection efficiency and accurate detection.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological detection technical field especially, relates to a fluorescence detection device based on quantum dot. BACKGROUND

[0002] Quantum fluorescence detection device is a kind of instrument using the fluorescence property of quantum dot material to carry out substance detection and analysis.Quantum dot is nanometer size semiconductor material, with unique optical property.When exciting quantum dot, they will emit fluorescence of specific wavelength.Fluorescence detection system is used to measure the fluorescence signal emitted by sample, and obtains the information of sample by analyzing these signals.

[0003] At present, when using quantum dot fluorescence detection, sample needs to be taken down single, which causes low detection efficiency, and the fluorescence detection device based on quantum dot disclosed in the prior art CN221926130U relates to detection device technical field, solves the problem of low efficiency when detecting multiple target materials, including installation platform, the surface of installation platform is installed with several groups of support plate, the top of support plate is installed with integrating sphere, the surface of integrating sphere is installed with light inlet and detection port, the surface of installation platform is installed with automatic replacement mechanism.The device can quickly detect multiple target substances by the cooperation of motor, threaded rod, sliding block, fluorescence detection machine, excitation light source, detection tube, first sliding connection ring, first electric cylinder, light-emitting tube, second sliding connection ring and second electric cylinder, greatly improves work efficiency.In actual use process, frequent docking operation is needed, and sample still needs to be single fed.

[0004] Therefore, it is necessary to provide a new fluorescence detection device based on quantum dot to solve the above technical problems. UTILITY MODEL CONTENT

[0005] To solve the above technical problems, the utility model provides a fluorescence detection device based on quantum dot.

[0006] The utility model provides a fluorescent detection device based on quantum dot includes: workstation and fixedly installed on the side plate and stand of workbench upper surface, fixedly installed with fluorescent detector on the side plate, install two groups of ring frame on the stand, fixedly installed with integrating sphere on two groups of ring frame, the top of integrating sphere installs the light pipe, the top of light pipe installs laser generator, the side close to side plate of integrating sphere is linked with detection tube, the detection end of detection tube and fluorescent detector passes through the duct intercommunication, the bottom of integrating sphere installs the sample introduction blind tube, the side of sample introduction blind tube towards stand is provided with sample introduction groove, install the automatic sample introduction subassembly with sample introduction groove cooperation on the stand, the automatic sample introduction subassembly includes rotary sleeve, rotary sleeve rotation is installed in the bottom of stand, and the top of rotary sleeve is fixedly installed with the turntable, the upper surface edge of turntable is evenly installed with a plurality of arc blocks of opening outward, the arc block and turntable form the cavity of placing.

[0007] Preferably, the arc block is adapted to the sample introduction groove.

[0008] Preferably, the outer side wall of the rotary sleeve is fixedly sleeved with a driven gear, a servo motor is fixedly installed on one side of the workbench, a driving gear is fixedly sleeved on the output shaft of the servo motor, the driving gear is engaged with the driven gear, the servo motor is electrically connected with a control box, and the control box is fixedly installed on the workbench.

[0009] Preferably, a filter assembly is installed on the detection tube.

[0010] Preferably, the filter assembly includes a connecting bracket, the connecting bracket is fixedly installed on the detection tube, one end of the connecting bracket is fixedly installed with a support shaft, a cutting disc is rotationally installed on the support shaft, a plurality of optical filters are uniformly embedded on the cutting disc, and a cutting groove adapted to the cutting disc is formed in the detection tube.

[0011] Preferably, a vertical plate is fixedly installed on the connecting bracket, a pin rod is slidingly installed on the vertical plate, an end disc is fixedly installed at the end of the pin rod, a return spring is sleeved on the pin rod, one end of the return spring is fixedly connected with the vertical plate, the other end of the return spring is fixedly connected with the end disc, and a plurality of positioning holes matched with the pin rod are formed in the cutting disc.

[0012] Compared with the related art, the fluorescent detection device based on quantum dots has the following beneficial effects:

[0013] 1. The utility model provides a fluorescent detection device based on quantum dots, through setting up the sampling groove at the sampling blind pipe of the integrating sphere, and setting up the automatic sampling assembly on the stand column, can realize efficient automatic sampling detection by the cooperation of the rotating sleeve, the rotating disc, the arc block, the placing cavity, the servo motor, the driving gear and the driven gear of automatic sampling assembly,

[0014] 2. Through setting up the filter disc assembly on the detection tube, the corresponding filter disc of filter disc assembly is switched by the cutting disc to filter the detected fluorescence, improve the precision of detection. DRAWINGS

[0015] Figure 1 It is a preferred embodiment structure diagram of the fluorescent detection device based on quantum dots provided by the utility model,

[0016] Figure 2 It is another perspective structure diagram of the fluorescent detection device based on quantum dots provided by the utility model,

[0017] Figure 3 It is the structure diagram of sampling blind pipe and rotating disc adhesion provided by the utility model,

[0018] Figure 4 It is the structure diagram of sampling blind pipe and rotating disc separation explosion provided by the utility model,

[0019] Figure 5 It is the structure diagram of filter disc assembly installed on the detection tube provided by the utility model.

[0020] Marked number in the drawing: 1, workbench;11, side plate;12, stand column;13, ring stand;2, fluorescent detector;3, integrating sphere;31, light pipe;32, detection tube;4, laser generator;5, sampling blind pipe;501, sampling groove;6, automatic sampling assembly;61, rotating sleeve;62, rotating disc;63, arc block;601, placing cavity;64, servo motor;65, driving gear;611, driven gear;7, filter disc assembly;71, connecting support;72, support shaft;73, cutting disc;74, filter disc;75, pin rod;76, end disc;77, return spring;711, vertical plate;701, positioning hole;8, control box. DETAILED DESCRIPTION

[0021] In order to make the utility model's purpose, technical scheme and advantage more clear and obvious, the following is combined with the drawing and example, and the utility model is further detailedly explained.It should be understood that the specific example described here is only used to explain the utility model, and is not used to limit the utility model.

[0022] The specific implementation of the utility model is described in detail below in combination with specific examples.

[0023] Please refer to Figures 1 to 5 The utility model discloses a fluorescent detection device based on quantum dots provides, fluorescent detection device based on quantum dots includes:

[0024] Workbench 1 and fixedly installed on the lateral plate 11 and the stand 12 of the upper surface of workbench 1, the lateral plate 11 is fixedly installed with fluorescent detector 2, the stand 12 is installed with two groups of ring frame 13, two groups of the ring frame 13 are fixedly installed with integrating sphere 3, the top of integrating sphere 3 is installed with light pipe 31, the top of light pipe 31 is installed with laser generator 4, the side close to lateral plate 11 of integrating sphere 3 is communicated with detection tube 32, detection tube 32 is communicated with the detection end of fluorescent detector 2 through the catheter, the bottom of integrating sphere 3 is installed with sample injection blind tube 5, the side towards stand 12 of sample injection blind tube 5 is provided with sample injection groove 501, and the stand 12 is installed with automatic sample injection assembly 6 matched with sample injection groove 501.

[0025] It should be noted that: when using, the sample to be detected is placed on the automatic sample injection assembly 6, sequentially passes through the sample injection groove 501, then the laser generator 4 is used to emit light from the light pipe 31, irradiate on the sample, then the fluorescent detector 2 is used to detect the fluorescence of the sample through the detection tube 32, which realizes continuous feeding detection and improves the detection efficiency.

[0026] In the embodiment of the utility model, please refer to Figures 1 to 5 The automatic sample injection assembly 6 includes rotating sleeve 61, rotating sleeve 61 is rotatably installed at the bottom of stand 12, and the top of rotating sleeve 61 is fixedly installed with rotating disc 62, the upper surface edge of rotating disc 62 is uniformly installed with a plurality of arc blocks 63 with opening outward, and the arc block 63 and rotating disc 62 form placing cavity 601;

[0027] The outer side wall of rotating sleeve 61 is fixedly provided with driven gear 611, one side of workbench 1 is fixedly installed with servo motor 64, the output shaft of servo motor 64 is fixedly provided with driving gear 65, driving gear 65 is engaged with driven gear 611, and servo motor 64 is electrically connected with control box 8, and control box 8 is fixedly installed on workbench 1.

[0028] It should be noted that: when using, the sample is sequentially placed in the placing cavity 601 formed by the arc block 63 and the rotating disc 62, then the servo motor 64 is controlled through the control box 8, and then the rotating disc 62 is driven to rotate through the engagement of driving gear 65 and driven gear 611, so that the sample in the placing cavity 601 is sequentially transferred into the sample injection groove 501 for detection.

[0029] It needs to be explained that: here, the control box 8 controls the servo motor 64 to work intermittently according to the single fluorescence detection time, so as to ensure that the sample is placed in the sample inlet groove 501 for a sufficient time for detection.

[0030] In the embodiment, the arc-shaped block 63 is matched with the sample inlet groove 501, that is, when the arc-shaped block 63 rotates into the sample inlet groove 501, the arc-shaped block 63 fills the entire sample inlet groove 501, so as to avoid large gaps and external light source interference.

[0031] In the embodiment of the utility model, please refer to Figures 1 to 5 The filter assembly 7 is installed on the detection tube 32, and the filter assembly 7 comprises a connecting bracket 71, the connecting bracket 71 is fixedly installed on the detection tube 32, one end of the connecting bracket 71 is fixedly installed with a supporting shaft 72, the supporting shaft 72 is rotatably installed with a cutting disc 73, the cutting disc 73 is uniformly embedded with a plurality of optical filters 74, and the cutting disc 73 is adapted with a cutting groove formed in the detection tube 32.

[0032] The connecting bracket 71 is fixedly installed with a vertical plate 711, the vertical plate 711 is slidably installed with a pin rod 75, the end of the pin rod 75 is fixedly installed with an end disc 76, the pin rod 75 is sleeved with a reset spring 77, one end of the reset spring 77 is fixedly connected with the vertical plate 711, and the other end of the reset spring 77 is fixedly connected with the end disc 76, and the cutting disc 73 is provided with a plurality of positioning holes 701 matched with the pin rod 75.

[0033] It needs to be explained that: in the detection of the detection tube 32 by the fluorescence detector 2, according to the selected spectrum type, the corresponding optical filter 74 is selected and switched to the cutting groove of the detection tube 32, when switching, the end disc 76 is first pulled outwards, so that the pin rod 75 is pulled out of the positioning hole 701, then the corresponding optical filter 74 is rotated into the cutting groove, and then the end disc 76 is loosened and automatically inserted into the corresponding positioning hole 701 under the action of the reset spring 77, and the switching and fixing are completed.

[0034] The working principle of the fluorescence detection device based on quantum dots is as follows:

[0035] In use, the sample is placed in the placement cavity 601 composed of the arc-shaped block 63 and the rotating disc 62 in sequence, then the servo motor 64 is controlled by the control box 8 to rotate, and then the driving rotating sleeve 61 drives the rotating disc 62 to rotate through the meshing of the driving gear 65 and the driven gear 611, so that the sample in the placement cavity 601 is sequentially transferred into the sample groove 501, and then the laser generator 4 is used to emit light from the light inlet tube 31 to irradiate on the sample, and then the fluorescence detector 2 is used to detect the fluorescence of the sample through the detection tube 32, so that the sample is automatically fed, the continuous feeding detection can be realized, and the detection efficiency is improved.

[0036] Further, when the fluorescence detector 2 is used to detect in the detection tube 32, the corresponding filter 74 is selected to switch into the cutting groove of the detection tube 32 according to the selected spectrum type, when switching, the end disc 76 is first pulled outwards, so that the pin rod 75 is pulled out of the positioning hole 701, then the corresponding filter 74 is rotated into the cutting groove, the end disc 76 is loosened and automatically inserted into the corresponding positioning hole 701 under the action of the reset spring 77, the switching and fixing are completed, and the detection accuracy can be further improved.

[0037] The circuit and the control involved in the utility model are prior art, and too much repetition is not performed here.

[0038] The above is only an embodiment of the utility model, and does not limit the patent range of the utility model, and any equivalent structure or equivalent process conversion obtained by using the content of the utility model specification and drawings, or direct or indirect application in other related technical fields, is also included in the patent protection range of the utility model.

Claims

1. A quantum dot-based fluorescence detection device, comprising: a workbench (1), and a side plate (11) and a column (12) fixedly installed on the upper surface of the workbench (1), characterized in that the side plate (11) is fixedly installed with a fluorescence detector (2), the column (12) is installed with two sets of ring frames (13), the two sets of ring frames (13) are fixedly installed with an integrating sphere (3), the top end of the integrating sphere (3) is installed with a light inlet tube (31), the top end of the light inlet tube (31) is installed with a laser generator (4), the side of the integrating sphere (3) close to the side plate (11) is communicated with a detection tube (32), the detection tube (32) is communicated with the detection end of the fluorescence detector (2) through a conduit, the bottom end of the integrating sphere (3) is installed with a sample inlet blind tube (5), the side of the sample inlet blind tube (5) facing the column (12) is provided with a sample inlet groove (501), the column (12) is installed with an automatic sample feeding assembly (6) matched with the sample inlet groove (501), the automatic sample feeding assembly (6) comprises a rotating sleeve (61), the rotating sleeve (61) is rotatably installed at the bottom end of the column (12), and the top end of the rotating sleeve (61) is fixedly installed with a rotating disc (62), the upper surface edges of the rotating disc (62) are uniformly installed with a plurality of arc-shaped blocks (63) with openings facing outward, and the arc-shaped blocks (63) and the rotating disc (62) form a placing cavity (601).

2. The quantum dot-based fluorescence detection device of claim 1, wherein, The arc-shaped blocks (63) are matched with the sample inlet groove (501).

3. The quantum dot-based fluorescence detection device of claim 1, wherein, The outer side wall of the rotating sleeve (61) is fixedly sleeved with a driven gear (611), one side of the workbench (1) is fixedly installed with a servo motor (64), the output shaft of the servo motor (64) is fixedly sleeved with a driving gear (65), the driving gear (65) is engaged with the driven gear (611), and the servo motor (64) is electrically connected with a control box (8), and the control box (8) is fixedly installed on the workbench (1).

4. The quantum dot-based fluorescence detection device of claim 1, wherein, A filter assembly (7) is installed on the detection tube (32).

5. The quantum dot-based fluorescence detection device of claim 4, wherein, The filter assembly (7) comprises a connecting bracket (71), the connecting bracket (71) is fixedly installed on the detection tube (32), one end of the connecting bracket (71) is fixedly installed with a supporting shaft (72), the supporting shaft (72) is rotatably installed with a cutting disc (73), a plurality of light filters (74) are uniformly embedded on the cutting disc (73), and a cutting groove matched with the cutting disc (73) is formed in the detection tube (32).

6. The quantum dot-based fluorescence detection device of claim 5, wherein, A vertical plate (711) is fixedly installed on the connecting bracket (71), a pin rod (75) is slidably installed on the vertical plate (711), an end disc (76) is fixedly installed at the end of the pin rod (75), a reset spring (77) is sleeved on the pin rod (75), one end of the reset spring (77) is fixedly connected with the vertical plate (711), the other end of the reset spring (77) is fixedly connected with the end disc (76), and a plurality of positioning holes (701) matched with the pin rod (75) are formed in the cutting disc (73).

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