Horizontal electrophoretic analysis device capable of quantifying by using 384-hole fluorescence microplate reader

Abstract

The utility model provides a horizontal electrophoretic analysis device capable of quantifying by using a 384-hole fluorescence microplate reader, which comprises a bottom box, a first glue preparation groove and a second glue preparation groove are respectively arranged on the bottom box, and a plurality of groups of limiting grooves are arranged on both sides of the first glue preparation groove and the second glue preparation groove; the jack comb is provided with clamping parts matched with the multiple sets of limiting grooves, first comb teeth and second comb teeth are arranged between the clamping parts respectively, and the first comb teeth and the second comb teeth are each provided with a first identification groove; and a supporting plate and an electrophoresis frame. According to the horizontal electrophoretic analysis device capable of quantifying by using the 384-hole fluorescence microplate reader, when glue is prepared, the tray is placed in the bottom box, glue is added, then the jack comb is clamped into the multiple groups of limiting grooves, and after the glue is solidified, the jack comb is taken out, and an electrophoresis frame is mounted for an electrophoresis experiment, so that the influence of movement of the tray on implementation data is reduced; and the accuracy of experimental data is improved.

Description

Technical Field

[0001] This utility model relates to the field of biological detection equipment technology, and more specifically to a horizontal electrophoresis analysis device that can be quantitatively analyzed using a 384-well fluorescence microplate reader. Background Technology

[0002] Agarose gel electrophoresis is used in analytical experiments in molecular biology, genetics, or genomics. In the experiment, agar or agarose is used as the supporting medium. The sample is placed in an agarose gel and TE buffer. Under the influence of negative charge, the sample moves from the negative charge to the positive charge. The pentose sugar-phosphate backbone in the sample is in a dissociated state. Due to the reproducibility of the structure, nucleic acid molecules of the same length have the same mass-to-charge ratio. Therefore, the main factor affecting the electrophoresis rate is the molecular sieving effect. For molecules with similar configurations, the larger the molecular weight, the slower the migration. Thus, the samples are separated according to their molecular weight.

[0003] A fluorescence microplate reader is mainly used to detect and analyze fluorescence signals in samples. Due to the differences in molecular fluorescence in the sample, this instrument can measure the characteristics and intensity of fluorescence, thereby enabling qualitative or quantitative analysis of substances.

[0004] During the electrophoresis gel preparation process, the colloid is poured onto a tray, and then combs are inserted. After the colloid solidifies, the combs are removed to form multiple grooves. Experimental samples are then poured into these grooves for electrophoresis. However, during the removal of the gel preparation tray, it is easy for hands to touch the colloid or for the gel surface to become unbalanced, which can affect the subsequent gel running operation and lead to inaccurate experimental data. Utility Model Content

[0005] The purpose of this invention is to solve the aforementioned problems in the existing technology.

[0006] To achieve the above objectives, this utility model can be implemented through the following technical solution: a horizontal electrophoresis analysis device that can be quantitatively analyzed using a 384-well fluorescence microplate reader, comprising:

[0007] The bottom box has a first glue-making groove and a second glue-making groove respectively. Both sides of the first glue-making groove and the second glue-making groove are provided with multiple sets of limiting grooves.

[0008] The plug comb has a snap-fit ​​part that cooperates with the multiple sets of limiting grooves. A first comb tooth and a second comb tooth are respectively provided between the snap-fit ​​parts. A first marking groove is provided on both the first comb tooth and the second comb tooth.

[0009] A tray, which is adapted to the first or second glue-making tank and cooperates with the first or second comb teeth;

[0010] An electrophoresis frame is mounted on the base box.

[0011] In this embodiment of the utility model, the bottom box is provided with an arc-shaped groove, and the tray is symmetrically provided with protrusions that cooperate with the arc-shaped groove, and the protrusions cooperate with the electrophoresis frame.

[0012] In this embodiment of the utility model, the pallet is an integrally molded part.

[0013] In this embodiment of the utility model, the electrophoresis frame is provided with a positive electrode mounting part and a negative electrode mounting part, and each of the positive electrode mounting part and the negative electrode mounting part is provided with an electrical control device, the electrical control device including an electrical sheet facing the tray;

[0014] The electrophoresis frame has a slot that matches the protrusion.

[0015] In this embodiment of the utility model, the electrical control unit further includes a support block, an adapter, and a plug that engages with the adapter. The electrical piece is snapped onto the support block, and the electrical piece abuts against the adapter.

[0016] In this embodiment of the invention, a liquid level sensor is provided on the support block.

[0017] In this embodiment of the utility model, the electrophoresis frame is provided with a liquid injection head, and a liquid addition block is provided at one end of the liquid injection head.

[0018] In this embodiment of the utility model, the electrophoresis frame is provided with a control panel, which cooperates with the electrophoresis control panel.

[0019] In this embodiment of the utility model, an observation plate is provided on the electrophoresis frame, and the observation plate is a transparent plate.

[0020] In this embodiment of the utility model, limit blocks are symmetrically arranged on the electrophoresis frame, and the limit blocks include a fitting part and an arc-shaped part that are connected to each other.

[0021] Compared with the prior art, the advantages of this application are: simple structure and reasonable design. When preparing the gel, the tray is placed in the bottom box, the gel is added, and the insertion comb is inserted into multiple sets of limiting grooves. After the gel solidifies, the insertion comb is removed and the electrophoresis rack is installed for electrophoresis experiment. This reduces the impact of tray movement on the data and improves the accuracy of experimental data. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall assembly structure;

[0023] Figure 2 This is a schematic diagram of the disassembled structure of the parts in the bottom box;

[0024] Figure 3This is a planar schematic diagram of the jack comb;

[0025] Figure 4 This is a schematic diagram showing the disassembled structure of the non-septate microplate and the tray;

[0026] Figure 5 This is a schematic diagram of the structure on which the electrophoresis frame is assembled on the box;

[0027] Figure 6 This is a schematic diagram of the exploded structure of some parts of the electrophoresis frame;

[0028] Figure 7 yes Figure 6 Enlarged view of part A in the middle.

[0029] Explanation of reference numerals in the attached figures:

[0030] 1. Base box; 11. Arc-shaped groove; 12. First gel casting tank; 13. Multiple sets of limiting grooves; 14. Second gel casting tank; 15. Drain hole; 2. Support plate; 21. Protrusion; 3. Insertion comb; 31. Snap-fit ​​part; 32. First marking groove; 33. First comb tooth; 34. Second comb tooth; 4. Gel casting plate; 5. Separatorless ELISA plate; 6. Electrophoresis rack; 61. Electrical control; 611. Liquid level sensor; 612. Electrical... 613. Plate; 614. Baffle; 615. Support block; 616. Adapter; 617. Plug; 62. Negative electrode mounting part; 63. Limiting block; 631. Fitting part; 632. Arc-shaped part; 64. Slot; 65. Slide groove; 66. Side plate; 661. Locking post; 67. Control panel; 674. Injection head; 675. Injection block; 68. Positive electrode mounting part; 681. Second marking slot; 69. Observation plate. Detailed Implementation

[0031] The following are specific embodiments of the present invention, and the technical solution of the present invention will be further described in conjunction with the accompanying drawings.

[0032] like Figure 1-7 As shown, a horizontal electrophoresis analysis device that can be quantitatively analyzed using a 384-well fluorescent microplate reader includes:

[0033] The bottom box 1 has a first glue-making groove 12 and a second glue-making groove 14 respectively. Multiple sets of limiting grooves 13 are provided on both sides of the first glue-making groove 12 and the second glue-making groove 14.

[0034] The insertion comb 3 has a locking part 31 that mates with multiple sets of limiting grooves 13. A first comb tooth 33 and a second comb tooth 34 are respectively provided between the locking parts 31. Each of the first comb tooth 33 and the second comb tooth 34 has a first marking groove 32, which is a letter from A to P (e.g., A to P). Figure 3 As shown), the letters correspond to the raised teeth of the first comb tooth 33 or the second comb tooth 34;

[0035] The tray 2 is adapted to the first glue-making tank 12 or the second glue-making tank 14 and cooperates with the first comb tooth 33 or the second comb tooth 34.

[0036] Electrophoresis frame 6 is mounted on the base box 1.

[0037] Specifically, the base box 1 has a first gel-forming tank 12 and a second gel-forming tank 14. Firstly, after one gel-forming cycle, a second gel-forming cycle can be performed directly, reducing cleaning frequency and improving experimental efficiency. Secondly, the first and second gel-forming tanks 12 and 14 can prepare colloids of different specifications. For example, multiple sets of limiting grooves 13 can be used to change the position of the insertion comb 3, thereby improving the adaptability of the device. During the electrophoresis experiment, the tray 2 or gel-forming plate 4 is placed in the base box 1 beforehand. The prepared colloid is then poured onto the tray 2, and before the colloid solidifies, the insertion comb 3 is engaged in the multiple sets of limiting grooves 1. Inside 3, after the colloid on tray 2 solidifies, remove the insertion comb 3. Due to the obstruction of the first comb tooth 33 or the second comb tooth 34, a uniformly distributed groove is formed. The experimental sample can be poured into the groove to wait for the gel running operation. Then, the electrophoresis rack 6 is clipped onto the base box 1, and electrophoresis buffer is added to the first gel preparation tank 12 for gel running, thereby reducing the impact of moving the tray on the experimental data. Furthermore, multiple sets of limiting grooves 13 are distributed on the outer wall of the base box 1. The limiting grooves cooperate with the insertion comb 3 to change the position of the insertion comb 3 to adapt to the electrophoresis experiment of colloids of different specifications.

[0038] As a further embodiment of this utility model, the bottom box 1 is provided with an arc-shaped groove 11, and the tray 2 is symmetrically provided with protrusions 21 that cooperate with the arc-shaped groove 11. The protrusions 21 cooperate with the electrophoresis rack 6. The protrusions 21 protrude from the arc-shaped groove 11 so that the tray 2 can be taken out from the bottom box 1 and placed on the unseparated ELISA plate 5. It can also be used for analysis by a fluorescence ELISA reader. By observing the fluorescence intensity in the experimental sample, the substance can be qualitatively or quantitatively analyzed, thereby improving the accuracy of the experimental data.

[0039] As a further embodiment provided by this utility model, the tray 2 is an integrally molded part, and both the tray 2 and the glue-making plate 4 are integrally molded parts, both of which are adapted to the bottom box 1.

[0040] As a further embodiment provided by this utility model, the electrophoresis frame 6 is provided with a positive electrode mounting part 68 and a negative electrode mounting part 62, respectively. Both the positive electrode mounting part 68 and the negative electrode mounting part 62 are provided with an electrical control 61. The electrical control 61 includes an electrode plate 612 facing the support plate 2. The electrophoresis frame 6 has a slot 64 that mates with the protrusion 21. The protrusion 21 on the support plate 2 restricts the electrophoresis frame 6, providing a certain degree of fixed support. Both the positive electrode mounting part 68 and the negative electrode mounting part 62 have a second marking groove 681, such as... Figure 6The indicated grooves are marked with "+" or "-", corresponding to the positive electrode mounting part 68 and the negative electrode mounting part 62. The negative electrode mounting part 62 is located on the side near the insertion comb 3. During the gel running process, the molecules of the experimental sample move from the negative electrode to the positive electrode to form a gel lane.

[0041] As a further embodiment of this utility model, the electrical control device 61 also includes a support block 614, an adapter 615, and a plug 616 that engages with the adapter 615. The electrode 612 is snapped onto the support block 614 and abuts against the adapter 615. A baffle 613 is provided on the support block 614 to fix the electrode 612. The electrical control device 61 is used to release positive and negative charges to facilitate electrophoresis experiments. The support block 614 supports the electrode 612, while the adapter 615 fixes it on the electrophoresis frame 6. When running the gel, the plug 616 can be inserted into the adapter 615 for gel running. After the experiment, the plug 616 can be unplugged to improve the electrical safety of the device.

[0042] As a further embodiment of this utility model, a liquid level sensor 611 is provided on the support block 614, and a drain hole 15 is provided on the bottom box 1. When running the gel, electrophoresis buffer is added to the bottom box 1, and the liquid level is detected by the liquid level sensor 611. Then, the experimental sample is added to the solidified gel on the tray 2, and then the gel running operation is performed. After the experiment is completed, the electrophoresis buffer can be drained through the drain hole 15, and the inner wall of the bottom box 1 is cleaned to facilitate subsequent experiments.

[0043] As a further embodiment of this utility model, the electrophoresis rack 6 is provided with a liquid injection head 674, one end of which is provided with a liquid addition block 675. The liquid injection head 674 is connected to an external infusion tube to inject electrophoresis buffer into the bottom box 1. The electrophoresis buffer is sprayed from the liquid addition block 675 onto the inner wall of the bottom box 1, thereby reducing the influence of the electrophoresis buffer on the coagulated colloid and improving the accuracy of experimental data.

[0044] As a further embodiment of this utility model, an electrophoresis stand 6 is provided with a control panel 67, which cooperates with the electrophoresis control 61. The control panel 67 has a display screen, a knob and an adjustment button, and controls the voltage of the electrophoresis control 61 and the electrophoresis time through the knob and the adjustment button.

[0045] As a further embodiment of this utility model, an observation plate 69 is provided on the electrophoresis rack 6. The observation plate 69 is a transparent plate, and the experimenter can observe the electrophoretic state of the experimental sample through the observation plate 69. The observation plate 69 can be removed from the electrophoresis rack 6, and the experimental sample can be added to the coagulated colloid through the removed opening.

[0046] As a further embodiment provided by this utility model, limit blocks 63 are symmetrically arranged on the electrophoresis frame 6. The limit blocks 63 include a mating part 631 and an arc-shaped part 632 connected to each other. Multiple sets of limit grooves 13 of the limit blocks 63 are engaged with each other. The mating part 631 abuts against the outer wall of the bottom box 1, while the arc-shaped part 632 is arranged to protrude. By bending the arc-shaped part 632, the limit blocks 63 can be disengaged from the multiple sets of limit blocks 63. A sliding groove 65 is provided on the electrophoresis frame 6. A side plate 66 is provided in the sliding groove 65. The side plate 66 fits against the arc-shaped groove 11 to improve the sealing performance during the electrophoresis experiment and further improve the fixing effect of the electrophoresis frame 6. When the position of the side plate 66 is determined, it can be fixed by locking post 661.

[0047] The above-described technical solution of this utility model addresses the problem that existing technical solutions are too simplistic and provides a solution that is significantly different from existing technologies. The parts not covered in this application's technical solution are the same as or can be implemented using existing technologies, and will not be described in detail here.

[0048] The technical solutions in the above embodiments have clearly and completely described the content of this utility model. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

Claims

1. A horizontal electrophoresis analysis device that can be quantitatively analyzed using a 384-well fluorescent microplate reader, characterized in that, include: The bottom box has a first glue-making groove and a second glue-making groove respectively. Both sides of the first glue-making groove and the second glue-making groove are provided with multiple sets of limiting grooves. The plug comb has a snap-fit ​​part that cooperates with the multiple sets of limiting grooves. A first comb tooth and a second comb tooth are respectively provided between the snap-fit ​​parts. A first marking groove is provided on both the first comb tooth and the second comb tooth. A tray, which is adapted to the first or second glue-making tank and cooperates with the first or second comb teeth; An electrophoresis frame is mounted on the base box.

2. The horizontal electrophoresis analysis device for quantitative analysis using a 384-well fluorescent microplate reader according to claim 1, characterized in that, The bottom box has an arc-shaped groove, and the tray has symmetrical protrusions that cooperate with the arc-shaped groove. The protrusions cooperate with the electrophoresis frame.

3. The horizontal electrophoresis analysis device for quantitative analysis using a 384-well fluorescent microplate reader according to claim 1, characterized in that, The pallet is a one-piece molded part.

4. The horizontal electrophoresis analysis device for quantitative analysis using a 384-well fluorescent microplate reader according to claim 2, characterized in that, The electrophoresis frame is provided with a positive electrode mounting part and a negative electrode mounting part, and each of the positive electrode mounting part and the negative electrode mounting part is provided with an electrical control device, the electrical control device including an electrical plate facing the tray; The electrophoresis frame has a slot that matches the protrusion.

5. A horizontal electrophoresis analysis device for quantitative analysis using a 384-well fluorescent microplate reader according to claim 4, characterized in that, The electrical control unit also includes a support block, an adapter, and a plug that engages with the adapter. The electrical contact is snapped onto the support block and abuts against the adapter.

6. A horizontal electrophoresis analysis device for quantitative analysis using a 384-well fluorescent microplate reader according to claim 5, characterized in that, A liquid level sensor is installed on the support block.

7. A horizontal electrophoresis analysis device for quantitative analysis using a 384-well fluorescent microplate reader according to claim 4, characterized in that, The electrophoresis frame is equipped with a liquid injection head, and a liquid addition block is provided at one end of the liquid injection head.

8. A horizontal electrophoresis analysis device for quantitative analysis using a 384-well fluorescent microplate reader according to claim 4, characterized in that, The electrophoresis stand is equipped with a control panel, which works in conjunction with the electrophoresis controls.

9. A horizontal electrophoresis analysis device for quantitative analysis using a 384-well fluorescent microplate reader according to claim 4, characterized in that, The electrophoresis stand is equipped with an observation plate, which is a transparent plate.

10. A horizontal electrophoresis analysis device for quantitative analysis using a 384-well fluorescent microplate reader according to claim 4, characterized in that, The electrophoresis frame is symmetrically provided with limiting blocks, each limiting block including an interlocking part and an arc-shaped part.

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