Portable low-temperature protein purification box

Abstract

The utility model relates to the technical field of protein purification, and particularly discloses a portable low-temperature protein purification box which comprises a box body, a control panel is mounted at the top of the box body, a temperature sensor is mounted on the inner wall of the box body, and a sample loading module, a purification module, a detection module and a sample collection module are arranged in the box body. The sample loading module comprises a sample liquid storage device, a buffer liquid storage device and a peristaltic pump which are mounted in the box body, the purification module comprises a chromatographic column connected with the peristaltic pump, and the detection module comprises an ultraviolet detector mounted in the box body. Through the design of the low-temperature control system and the heat preservation layer, protein purification can be carried out in a low-temperature environment, the activity of the protein is effectively maintained, and the problem that the protein is inactivated or even degraded due to the fact that the temperature is too high in a traditional purification method is solved; meanwhile, the influence of long-time low-temperature experiments on the body health of experiment operators is avoided.

Description

Technical Field

[0001] This invention belongs to the field of protein purification technology, specifically relating to a portable low-temperature protein purification box. Background Technology

[0002] Protein purification is a technical process that separates and extracts target proteins from biological samples and removes impurities. It is an indispensable step in biochemical research and biopharmaceutical manufacturing.

[0003] Traditional protein purification methods are typically performed in laboratory environments and require relatively high temperatures, especially at low temperatures where protein activity is less likely to be destroyed. However, most existing protein purification equipment is bulky and complex to operate, making it inconvenient to carry around and use in the field. This is particularly true for the purification of proteins with high temperature requirements, such as enzymes, where excessively high temperatures can easily lead to loss of activity. In summary, existing purification systems limit the application of protein purification technology in on-site and mobile laboratory settings, and also have limitations in the purification of proteins at low temperatures.

[0004] In addition, traditional protein purification equipment usually requires the assembly and operation of multiple independent components, which not only increases the complexity of the operation, but also requires high operating skills from the operators, and can easily lead to protein inactivation or contamination during the purification process due to improper operation.

[0005] Therefore, developing an integrated protein purification device that can achieve low-temperature protein purification and is easy to carry has important application value. Utility Model Content

[0006] The purpose of this invention is to provide a portable low-temperature protein purification box to solve the problem that most existing protein purification equipment is bulky, complex to operate, and inconvenient to carry.

[0007] To achieve the above objectives, this utility model provides the following technical solution:

[0008] A portable low-temperature protein purification chamber includes a chamber body, a control panel mounted on the top of the chamber body, a temperature sensor mounted on the inner wall of the chamber body, and a sample loading module, a purification module, a detection module, and a sample collection module inside the chamber body. The sample loading module includes a sample liquid reservoir, a buffer reservoir, and a peristaltic pump installed inside the chamber body. The purification module includes a chromatographic column connected to the peristaltic pump. The detection module includes an ultraviolet detector installed inside the chamber body. The sample collection module includes a circular sample collector connected to the ultraviolet detector.

[0009] Preferably, the front of the box is equipped with two doors, each with an observation window, and the top of the box is fixedly connected with a handle.

[0010] Preferably, the top of both the sample liquid reservoir and the buffer solution reservoir is connected to a first L-shaped tube, the two first L-shaped tubes are connected to a common conduit at their close ends, the end of the conduit away from the first L-shaped tube is connected to a peristaltic pump, and valves are installed on the arc surfaces of both first L-shaped tubes.

[0011] Preferably, the top of the chromatographic column is connected to a second L-shaped tube, the end of the second L-shaped tube away from the chromatographic column is connected to a peristaltic pump, and the bottom of the chromatographic column is connected to a third L-shaped tube.

[0012] Preferably, the end of the third L-shaped tube furthest from the chromatographic column is connected to the ultraviolet detector.

[0013] Preferably, the end of the circular sample collector near the ultraviolet detector is connected to a first connecting tube, the end of the first connecting tube away from the circular sample collector is connected to the ultraviolet detector, the arc surface of the first connecting tube is connected to a second connecting tube, and the other end of the second connecting tube is connected to a waste liquid bottle, which is installed inside the housing.

[0014] Preferably, the inner wall of the enclosure is provided with a heat insulation layer, a heat-conducting copper plate is installed on the back of the enclosure, a semiconductor cooling chip is fixedly installed on the side of the heat-conducting copper plate away from the enclosure, and multiple heat sinks are fixedly installed on the side of the semiconductor cooling chip away from the heat-conducting copper plate.

[0015] Compared with the prior art, the beneficial effects of this utility model are:

[0016] 1. This utility model, through the design of a low-temperature control system and insulation layer, enables protein purification in a low-temperature environment, effectively maintaining the activity of the protein. It solves the problem of protein inactivation or even degradation caused by excessively high temperatures in traditional purification methods, and also addresses the impact of prolonged low-temperature experiments on the health of the experimenters.

[0017] 2. The protein purification device of this utility model features an integrated box design, which integrates the components required for protein purification into one box. It organically combines functions such as low temperature control, purification operation, and portability to form a complete system, achieving high efficiency, portability, and applicability of low temperature protein purification. It can reduce operation steps, reduce the operation requirements and difficulty for users, and is also convenient for carrying out and on-site use. Attached Figure Description

[0018] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0019] Figure 2 This utility model Figure 1 A schematic diagram of the cross-sectional planar structure;

[0020] Figure 3 This utility model Figure 2 Schematic diagram of the structure at point A in the middle;

[0021] Figure 4 This utility model Figure 1 A partial right-view structural diagram.

[0022] In the diagram: 1. Box body; 101. Box door; 102. Observation window; 103. Handle; 104. Control panel; 105. Insulation layer; 106. Thermally conductive copper plate; 107. Semiconductor cooling chip; 108. Heat sink; 109. Temperature sensor; 2. Sample loading module; 201. Sample liquid storage container; 202. Buffer solution storage container; 203. First L-shaped tube; 204. Valve; 205. Tubing; 206. Peristaltic pump; 3. Purification module; 301. Second L-shaped tube; 302. Chromatographic column; 303. Third L-shaped tube; 4. Detection module; 401. Ultraviolet detector; 5. Sample collection module; 501. First connecting tube; 502. Circular sample collector; 503. Second connecting tube; 504. Waste liquid bottle. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Reference Figures 1-4 As shown, this utility model provides a portable low-temperature protein purification box, including a box body 1. A control panel 104 is installed on the top of the box body 1, and a temperature sensor 109 is installed on the inner wall of the box body 1. The box body 1 is equipped with a sample loading module 2, a purification module 3, a detection module 4, and a sample collection module 5. The sample loading module 2 includes a sample liquid storage container 201, a buffer storage container 202, and a peristaltic pump 206 installed inside the box body 1. The purification module 3 includes a chromatographic column 302 connected to the peristaltic pump 206. The detection module 4 includes an ultraviolet detector 401 installed inside the box body 1. The sample collection module 5 includes a circular sample collector 502 connected to the ultraviolet detector 401.

[0025] In this embodiment, the control panel 104 can set the temperature inside the enclosure 1. The temperature sensor 109 is wired to the control panel 104 and detects the temperature inside the enclosure 1 in real time and feeds it back to the control panel 104.

[0026] In an optional embodiment: two doors 101 are installed on the front of the box 1, and observation windows 102 are installed on the surface of both doors 101. A handle 103 is fixedly connected to the top of the box 1.

[0027] It should be noted that the observation window 102 is used to facilitate staff to view the inside of the box 1, and the handle 103 is used to facilitate staff to carry the box 1.

[0028] In an optional embodiment: the top of both the sample liquid reservoir 201 and the buffer reservoir 202 are connected to a first L-shaped tube 203, the ends of the two first L-shaped tubes 203 that are close to each other are connected to a conduit 205, the end of the conduit 205 that is away from the first L-shaped tube 203 is connected to a peristaltic pump 206, and valves 204 are installed on the arc surfaces of both first L-shaped tubes 203.

[0029] The top of the chromatographic column 302 is connected to a second L-shaped tube 301. The end of the second L-shaped tube 301 away from the chromatographic column 302 is connected to a peristaltic pump 206. The bottom of the chromatographic column 302 is connected to a third L-shaped tube 303. The end of the third L-shaped tube 303 away from the chromatographic column 302 is connected to an ultraviolet detector 401.

[0030] The end of the circular sample collector 502 near the ultraviolet detector 401 is connected to a first connecting tube 501. The end of the first connecting tube 501 away from the circular sample collector 502 is connected to the ultraviolet detector 401. The arc surface of the first connecting tube 501 is connected to a second connecting tube 503. The other end of the second connecting tube 503 is connected to a waste liquid bottle 504. The waste liquid bottle 504 is installed inside the housing 1 and is used to collect waste liquid.

[0031] It should be noted that the ultraviolet detector 401 and the circular sample collector 502 are existing devices, and will not be described in detail here.

[0032] In an optional embodiment: the inner wall of the housing 1 is provided with a heat insulation layer 105, a heat-conducting copper plate 106 is installed on the back of the housing 1, the heat-conducting copper plate 106 is embedded inside the housing, a semiconductor cooling chip 107 is fixedly installed on the side of the heat-conducting copper plate 106 away from the housing 1, and a plurality of heat sinks 108 are fixedly installed on the side of the semiconductor cooling chip 107 away from the heat-conducting copper plate 106.

[0033] It should be noted that the insulation layer 105 is made of polyurethane foam material, which has a low thermal conductivity and excellent insulation performance.

[0034] By energizing the semiconductor cooling chip 107, a cooling effect can be achieved. With the help of the thermal conductivity of the heat-conducting copper plate 106, the heat inside the box 1 is dissipated, and the temperature inside the box 1 is reduced. The heat sink 108 plays a role in improving the heat dissipation effect.

[0035] The working principle of this utility model is as follows: In use, the desired low-temperature environment is first set via the control panel 104. The cooling device is activated, energizing the semiconductor cooling chip 107 to achieve cooling, transferring temperature to the heat-conducting copper plate 106 to lower the temperature inside the chamber 1. The temperature sensor 109 monitors the temperature inside the chamber in real time and feeds the data back to the control panel 104 to ensure the temperature is maintained at the set value. The insulation layer 105 keeps the ambient temperature inside the chamber 1 relatively stable. The protein purification process begins at low temperature. First, the sample to be purified is loaded into the sample solution reservoir 201. The sample solution in the sample solution reservoir 201 is slowly injected into the chromatographic column 302 at a constant rate by the peristaltic pump 206. The buffer solution in the buffer reservoir 202 flows into the chromatographic column 302 at a constant rate under the action of the peristaltic pump 206, and elutes with the protein sample. The eluted liquid is detected by the ultraviolet detector 401 to detect the elution of protein. After the ultraviolet detector 401 shows that protein has eluted, the eluted liquid is collected by the circular sample collector 502. The entire process is completed at low temperature to ensure that the activity of the protein is maintained.

[0036] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A portable low temperature protein purification box comprising a box body (1), characterized in that, The top of the box (1) is provided with a control panel (104), the inner wall of the box (1) is provided with a temperature sensor (109), the inside of the box (1) is provided with a sample loading module (2), a purification module (3), a detection module (4) and a sample collection module (5), the sample loading module (2) comprises a sample liquid reservoir (201), a buffer reservoir (202) and a peristaltic pump (206) installed in the box (1), the purification module (3) comprises a chromatographic column (302) connected with the peristaltic pump (206), the detection module (4) comprises an ultraviolet detector (401) installed in the box (1), and the sample collection module (5) comprises a circular sample collector (502) connected with the ultraviolet detector (401).

2. The portable low temperature protein purification box according to claim 1, characterized in that: The front of the box (1) is provided with two box doors (101), and the surfaces of the two box doors (101) are provided with observation windows (102), and the top of the box (1) is fixedly connected with a handle (103).

3. The portable low temperature protein purification box according to claim 1, characterized in that: The top of the sample liquid reservoir (201) and the buffer reservoir (202) is communicated with a first L-shaped pipe (203), and the two first L-shaped pipes (203) are communicated with a conduit (205) at one end close to each other, the conduit (205) is communicated with the peristaltic pump (206) at one end away from the first L-shaped pipe (203), and the arc surfaces of the two first L-shaped pipes (203) are provided with valves (204).

4. The portable low temperature protein purification box according to claim 1, characterized in that: The top end of the chromatographic column (302) is communicated with a second L-shaped pipe (301), the second L-shaped pipe (301) is communicated with the peristaltic pump (206) at one end away from the chromatographic column (302), and the bottom of the chromatographic column (302) is communicated with a third L-shaped pipe (303).

5. A portable low temperature protein purification box according to claim 4, characterized in that: The third L-shaped pipe (303) is communicated with the ultraviolet detector (401) at one end away from the chromatographic column (302).

6. The portable low temperature protein purification box of claim 1, wherein: The circular sample collector (502) is communicated with a first connecting pipe (501) at one end close to the ultraviolet detector (401), the first connecting pipe (501) is communicated with the ultraviolet detector (401) at one end away from the circular sample collector (502), the arc surface of the first connecting pipe (501) is communicated with a second connecting pipe (503), the other end of the second connecting pipe (503) is communicated with a waste liquid bottle (504), and the waste liquid bottle (504) is installed in the box (1).

7. The portable low temperature protein purification box according to claim 1, characterized in that: The inner wall of the box (1) is provided with a heat preservation layer (105), the back of the box (1) is provided with a heat conducting copper plate (106), the side of the heat conducting copper plate (106) away from the box (1) is fixedly provided with a semiconductor refrigerating sheet (107), and the side of the semiconductor refrigerating sheet (107) away from the heat conducting copper plate (106) is fixedly provided with a plurality of heat dissipation fins (108).

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