Sample collection filter bottle
By designing a connecting chamber and filter element inside the sampling rod, combined with a sample collection filter bottle containing an activated carbon layer, the inconvenience of use and pigment interference in fecal sample testing are solved, achieving clear dripping of the sample solution and accurate test results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-30
- Publication Date
- 2026-03-06
AI Technical Summary
Existing technologies for testing fecal samples suffer from inconvenience, risk of contamination, and interference with test results, especially since pigments in fecal samples have a significant impact on test results.
A sample collection filter bottle was designed, which includes a connected chamber, a first filter element, and a second filter element inside a sampling rod. The first filter element is provided with an activated carbon layer. Through the combination of multiple liquid inlet holes and filter elements, the pigment in the sample solution is filtered to reduce the pigment content.
This method achieves clear dripping of the sample solution, reduces background interference in the test results, and improves the readability and accuracy of the test results.
Smart Images
Figure CN223976957U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the technical field of medical testing equipment, and in particular to a sample collection filter bottle for collecting and processing feces. Background Technology
[0002] Currently, there are no commercially available hepatitis A virus antigen detection kits. Antigen detection is performed by individual manufacturers or research institutions using different methods based on their specific needs. Common laboratory detection methods include virus isolation and culture, reverse transcription-polymerase chain reaction (RT-PCR), and enzyme-linked immunosorbent assay (ELISA). These methods have drawbacks, such as being time-consuming, requiring specialized equipment and significant technical support, which poses challenges to HAV diagnosis. Colloidal gold immunochromatography, developed in the 1980s, is an immunoassay technique with advantages such as speed, simplicity, and intuitive results, and it holds great promise for application in disease detection.
[0003] Feces, as human waste, serve as a testing sample for indicators related to bacterial and viral infections, offering advantages such as minimal harm to the human body and convenient sampling. However, due to the complex composition and significant individual variations in fecal samples, and the low concentration of the substances to be tested, a small sample is typically collected from the raw feces using a sampling swab before testing. The sampling swab is then inserted into a sampling bottle to dilute and mix with the processing solution inside the bottle. Finally, a certain amount of the mixture is taken for testing.
[0004] When collecting stool samples, sampling swabs are typically used alone. After collecting the sample, they are inserted into the bottle to mix with the processing solution before being removed and discarded. This is not only inconvenient but also increases the risk of sample spillage or dripping onto the testing platform, causing contamination. Furthermore, due to the high complexity and heterogeneity of stool samples, pretreatment is usually necessary to remove potential interfering substances such as food residue, microorganisms, sloughed epithelial cells, and mucus. For test strips that use immunochromatography to detect target substances in stool, while residues in the stool sample solution can be retained by the sample pad, pigments in the stool sample can still interfere with the background of the test strip, affecting the interpretation of results and reducing testing efficiency. Utility Model Content
[0005] To solve the above-mentioned technical problems, this utility model provides a sample collection filter bottle. By opening a chamber connected to the dropper inside the sampling rod, and providing a first filter and a second filter with a built-in activated carbon layer on the sampling rod, the pigments of the sample solution are filtered through the first filter and the second filter, making the sample solution clearer and allowing the test personnel to read the test results conveniently without affecting the test results.
[0006] The sample collection filter bottle includes a bottle body, a dropper, a cap, and a sampling rod. One end of the sampling rod is fixedly connected to the dropper. The sampling rod has a hollow chamber along its axial direction. The top of the chamber has an opening that communicates with the dropper. The bottom of the chamber is closed to form a sampling section. The sampling rod is provided with a first filter element and a second filter element. The first filter element is located on the outer side of the sampling rod near the bottom of the dropper. The second filter element is located on the outer side of the sampling rod near the sampling section.
[0007] A further technical solution is: the outer surface of the first filter element is provided with multiple filter holes, and the side wall of the sampling rod connected to the first filter element is provided with several liquid inlet holes that communicate with the chamber.
[0008] After the sampling rod extracts the sample, it is inserted into the bottle. By shaking the bottle to dissolve the sample into the liquid inside the bottle and form a sample solution, the activated carbon layer inside the second filter adsorbs at least some of the pigments in the sample solution, thereby further reducing the pigment content in the dripped sample solution. Then, the sample collection filter bottle is inverted so that the dropper is facing down. The sample solution drips out from the dropper through the first filter and the inlet hole and chamber on the sampling rod. The activated carbon layer inside the first filter adsorbs at least some of the pigments in the sample solution, thereby reducing the pigment content in the dripped sample solution.
[0009] A further technical solution is to provide at least two liquid inlet holes with different directions or heights on the side wall of the sampling rod.
[0010] A further technical solution is to provide activated carbon layers in both the first and second filter elements.
[0011] A further technical solution is: the first filter element and the second filter element are fixed around the outer wall of the sampling rod and the cross-section is set to be circular, so that the sample solution can only enter the chamber of the sampling rod through the first filter element.
[0012] A further technical solution is to use activated carbon cotton mesh or activated carbon filter cartridges for the activated carbon layer.
[0013] A further technical solution is that a filter cake layer is also provided inside the first filter element, and the filter cake layer is wrapped around the outside of the activated carbon layer.
[0014] A further technical solution is to use fiberglass, sponge, or degreased cotton to make the filter cake layer.
[0015] A further technical solution is to set the surface of the sampling part as a spiral surface with a threaded structure; or to set the surface with multiple evenly distributed protrusions.
[0016] A further technical solution is to wrap the surface of the sampling section with cotton or sponge.
[0017] Beneficial effects
[0018] This application features a chamber inside the sampling rod that communicates with the dropper, and an inlet hole on the side wall of the sampling rod that communicates with the chamber. During use, simply tilting the bottle allows the sample liquid to flow out, eliminating the need to remove and discard the sampling rod, making it more convenient and hygienic. This application incorporates a first filter element on the outside of the sampling rod, containing a filter residue layer and an activated carbon layer arranged sequentially from the outside in. The filter residue layer filters out large particles of residue in the sample liquid, preventing blockage of the inlet hole and the chamber, ensuring smooth flow of the sample liquid from the dropper. The activated carbon's strong adsorption properties trap pigment molecules in the fecal sample, reducing interference from the background and facilitating the reading of test results on the test strip. By placing multiple inlet holes at different directions or heights on the side wall of the sampling rod, this application creates multiple different sample liquid flow paths. Combined with the annular first filter element, this maximizes the filtration efficiency of the first filter element. Attached Figure Description
[0019] Figure 1 This is a schematic diagram of the sample collection filter bottle of this utility model.
[0020] Figure 2 This is a schematic diagram of a sampling rod.
[0021] Figure 3 This is a cross-sectional view of the sampling rod.
[0022] Figure 4 This is a schematic diagram of the second filter element.
[0023] Figure 5 yes Figure 3 A schematic diagram of one implementation at point A.
[0024] Figure 6 yes Figure 3 A schematic diagram of another embodiment at point A.
[0025] Figure 7 yes Figure 3 A schematic diagram of one implementation at point B.
[0026] Figure 8 yes Figure 3 A schematic diagram of another implementation at point B.
[0027] Figure label:
[0028] 10. Sample collection filter bottle, 1. Bottle body, 2. Dropper, 21. Connecting part, 22. Second connecting part, 9. Cap, 3. Sampling rod, 31. Sampling part, 32. Chamber, 33. Liquid inlet, 4. First filter element, 41. Filter hole, 5. Activated carbon layer, 6. Filter residue layer, 7. Cotton, 8. Second filter element. Detailed Implementation
[0029] like Figure 1 The sample collection filter bottle 10 shown includes a bottle body 1, a dropper 2 disposed on the top of the bottle body 1, and a sampling rod 3 inserted inside the bottle body 1.
[0030] The bottle body 1 has an opening at the top, and the outer wall of the opening is threaded. The dropper 2 is threadedly connected to the opening at the top of the bottle body 1 via a connecting part 21. A second connecting part 22 is also provided on the connecting part 21. The connecting part 21, the second connecting part 22, and the dropper 23 are coaxially arranged. The dropper 23 has a through cavity along its axial direction, which communicates with the bottle body 1, allowing the sample liquid inside the bottle body 1 to flow out from the cavity. A cap 9 for sealing the dropper 2 is threadedly connected to the outer wall of the second connecting part 22.
[0031] like Figures 1-4 As shown, the top of the sampling rod 3 extends into the bottom of the dropper 2 and is fixedly connected to it. The sampling rod 3 has a hollow chamber 32 along its axial direction. The top of the chamber 32 has an opening and is connected to the dropper 23 of the dropper 2. The bottom of the chamber 32 is closed to form a sampling part 31. The section of the sampling rod 3 near the bottom of the dropper 2 is wrapped with a first filter element 4. The outer surface of the first filter element 4 has multiple filter holes 41. The interior of the first filter element 4 is provided with an activated carbon layer 5. Several liquid inlet holes 33 connected to the chamber 32 are opened on the side wall of the section of the sampling rod 3 wrapped with the first filter element 4.
[0032] In one embodiment, the top of the sampling rod 3 is integrally connected to the bottom of the dropper 2. In another embodiment, a downwardly extending connecting tube is provided inside the dropper 2, and the interior of the connecting tube is interconnected with the cavity of the dropper 23. The sampling rod 3 is snapped into the interior of the connecting tube, so that the chamber 32 of the sampling rod 3 is interconnected with the connecting tube.
[0033] The number of liquid inlet holes 33 can be set to one or more. In a preferred embodiment, such as... Figure 5 As shown, the sampling rod 3 has at least two inlet holes 33 with different directions or heights on its side wall. The sample liquid can enter the chamber 32 through the first filter element 4 from multiple different paths, so that the sample liquid is fully filtered and the flow rate of the sample liquid is increased.
[0034] The number of filter elements can be set to one. In one embodiment, the first filter element 4 is disposed on the sampling rod 3 near the bottom of the dropper 2. After the sample and the treatment liquid are thoroughly mixed, the sample collection filter bottle 100 is poured out, and the sample liquid in the bottle 1 is filtered through the first filter element 4 before flowing out from the dropper 2. Furthermore, after the sampling rod 3 extracts the sample and inserts it into the bottle 1, the sample dissolves in the liquid in the bottle to form a sample solution. Then, the sample collection filter bottle is inverted so that the dropper is facing down, and the sample solution drips out from the dropper 23 through the first filter element 4 and the liquid inlet hole 33 and chamber 32 on the sampling rod. The activated carbon layer 5 inside the first filter element adsorbs at least some of the pigments in the sample solution, thereby reducing the pigment content in the dripped sample solution.
[0035] To achieve better filtration results, multiple filter elements can be installed. For example... Figure 4 As shown, in another embodiment, in addition to the first filter element 4, a second filter element 8 is also provided on the outer side of the sampling rod 3 near the sampling part 31. The outer surface of the second filter element 8 has multiple filter holes, and an activated carbon layer is disposed inside the second filter element 8. During the process of the sampling rod 3 extracting the sample and inserting it into the bottle 1, and shaking the bottle 1 to dissolve the sample into the liquid inside the bottle to form a sample solution, the activated carbon layer inside the second filter element 8 adsorbs at least some of the pigment in the sample solution, thereby further reducing the pigment content in the dripped sample solution. This allows for a first filtration by the first filter element 4 near the sampling part 31 when the fecal sample is mixed with the treatment liquid in the bottle 1 by shaking the bottle 1, and a second filtration by the first filter element 4 near the dropper 23 when pouring, resulting in better pigment filtration.
[0036] The first filter element 4 and the second filter element 8 are fixedly surrounding the outer wall of the sampling rod 3. The cross-sections of the first filter element 4 and the second filter element 8 can be square, trapezoidal, or triangular. In a preferred embodiment, the cross-sections of the first filter element 4 and the second filter element 8 are circular, which increases the contact area between the activated carbon layer 5 and the sample solution, allowing the sample solution to be fully filtered. Furthermore, the sample solution can only enter the chamber 32 of the sampling rod through the first filter element 4.
[0037] The diameter of the filter holes 41 on the first filter element 4 and the second filter element 8 should be such that the activated carbon layer 5 does not fall out. Specifically, the activated carbon layer 5 can be filled with activated carbon cotton mesh or activated carbon filter cartridges. Through the rich porosity and strong adsorption properties of the activated carbon, the sample liquid is filtered, and pigment molecules in the fecal sample are trapped by physical adsorption.
[0038] The activated carbon layer 5 can be made from a single piece of activated carbon, processed into a shape that conforms to the inner cavity of the first / second filter element, such as a ring or a hollow cylinder, and then assembled into the first / second filter element; alternatively, it can be made from powdered raw materials and binders through kneading, extrusion molding, carbonization, activation, and other processes to form a shape that conforms to the inner cavity of the first / second filter element; or it can be made from powdered activated carbon with binders through extrusion molding and then assembled into the first / second filter element. The first / second filter element is a hollow cylindrical shape that is fitted and fixed onto the sampling rod 3.
[0039] Furthermore, such as Figure 6 As shown, the first filter element 4 also has a filter cake layer 6 inside, which wraps around the outside of the activated carbon layer 5. By setting the filter cake layer 6 to pretreat the sample solution, large fecal particles in the sample solution can be filtered out first, avoiding clogging of the first filter element 4 and the liquid inlet 33, thus preventing the flow of the sample solution from being affected. Specifically, the filter cake layer 6 is made of glass fiber, sponge, or degreased cotton.
[0040] The sampling rod 3 has a sampling section 31 at its bottom end. To ensure that the sampling section 31 can pick up a sufficient amount of sample at once, the surface of the sampling section 31 is designed to be uneven or irregular, enhancing adhesion to the sample and preventing it from easily falling off during handling. Specifically, in one embodiment, such as... Figure 7 As shown, the surface of the sampling section 31 is configured as a spiral surface with a threaded structure, giving the surface of the sampling section 31 multiple spiral grooves. This allows for the collection of more fecal samples, avoids multiple operations by the sampler, reduces discomfort, and prevents samples from falling out during handling, thus reducing contamination. In an optional embodiment, the surface of the sampling section 31 may be configured with multiple evenly distributed protrusions, which can retain a sufficient amount of fecal sample in the gaps between the protrusions. In another optional embodiment, such as... Figure 7 As shown, the surface of the sampling section 31 may also be covered with cotton 7 or sponge, so that a sufficient amount of fecal sample can be collected at once by utilizing the water absorption of cotton 7 or sponge.
[0041] The body 1, dropper 2, and sampling rod 3 of the sample collection filter bottle 10 are all made of polyethylene material. The body 1 of the sample collection filter bottle 10 is transparent or semi-transparent, which facilitates observation of the internal condition of the sample collection filter bottle.
[0042] To facilitate the smooth outflow of sample liquid from the sample collection filter bottle, a portion of the bottle body 1 can be designed to be elastic, allowing for easy compression and deformation of the bottle body 1. This accelerates the flow of sample liquid within the bottle body 1 towards the chamber 32 of the sampling rod 3 and out through the dropper 23. In one embodiment, the bottle body 1 can be cylindrical. In another embodiment, the bottle body 1 is shaped like a frustum with a cross-section that gradually increases from bottom to top, allowing the testing personnel to squeeze the tail of the bottle body 1, thus accelerating the flow of sample liquid inside the bottle body 1.
[0043] In use, the present invention is used to unscrew the dropper 2, collect a sufficient amount of fecal sample through the sampling rod 3, add the collected fecal sample to the sample collection filter bottle 10, mix it thoroughly with the sample processing liquid in the bottle body 1 to obtain the sample liquid, then pour the bottle body 1, so that the sample liquid passes through the first filter element 4 and enters the chamber 32 through the liquid inlet 33, and drips onto the test strip through the dropper 23 through the chamber 32.
[0044] Detection and experimental verification
[0045] (I) Preparation of Testing Materials
[0046] 1. Experimental group: Sample collection filter bottle with first filter element
[0047] 2. Control group: Sample collection filter bottles without the first filter element.
[0048] 3. Ten stool samples were collected from Hangzhou Adicon Medical Laboratory Center: five negative samples, designated N1, N2, N3, N4, and N5; and five positive samples, designated P1, P2, P3, P4, and P5. These ten samples were tested simultaneously in both the experimental and control groups.
[0049] 4. Validation Product: Hepatitis A Antigen Test Strips
[0050] (1) Test strip components:
[0051] Quality control area (C): Goat anti-mouse IgG polyclonal antibody
[0052] Test area (T): HAV antibody-1
[0053] Label pad: HAV antibody-2
[0054] Sample processing solution: Sodium chloride (0.09%) + EDTA (0.5%)
[0055] (2) Preparation of test strips:
[0056] The test strip includes a sample pad, a marking pad, a test pad, an absorbent pad, and a base card. The absorbent pad is made of absorbent paper.
[0057] Test pad preparation. The test pad uses a nitrocellulose membrane (NC membrane) as a carrier. The NC membrane has two lines: a test line (T line) and a control line (C line). The test line (T line) is immobilized with HAV antibody-1 at a concentration of 1.0 mg / ml, diluted with PB buffer at a pH of 7.0–8.0. The control line (C line) is immobilized with goat anti-mouse IgG polyclonal antibody at a concentration of 0.5 mg / ml, diluted with PB buffer at a pH of 7.0–8.0. The T line and C line are immobilized on the NC membrane using a membrane application machine. After processing, the test pad is dried in a 37°C oven.
[0058] Labeling pad preparation: Labeling pad 202 uses glass fiber as a carrier. The labeling solution is applied to the glass fiber using a spray dotting machine. The labeling solution is obtained by covalently coupling HAV antibody-2 with latex microspheres, with a concentration of 0.2%. The dilution buffer is 0.05M Tris + 0.5% BSA. After spray dotting, the labeling pad is dried in a 37℃ oven.
[0059] Sample pad preparation. Sample pad 201 uses glass fiber as a carrier. The sample pad treatment solution is sprayed onto the glass fiber. The sample pad treatment solution is: 0.05M Tris + 0.5% Tween-20 + 0.5% BSA + 0.2% Proclin 300. The treated sample pad is then dried in a 37℃ oven.
[0060] Assemble the test strips. Assemble the processed components, starting with the test pad. One end of the absorbent pad covers the downstream end of the test pad, the downstream end of the marker pad covers the upstream end of the test pad, and the downstream end of the sample pad covers the upstream end of the marker pad. Secure the assembled sample pad, marker pad, test pad, and absorbent pad to the base plate.
[0061] (3) Test strip principle
[0062] A highly specific antigen-antibody reaction and immunochromatographic technique is used to qualitatively detect the presence of HAV antigen in fecal samples. The reagent contains HAV antibody-1 pre-immobilized on a nitrocellulose membrane in the test zone (T line) and goat anti-mouse IgG polyclonal antibody in the control zone (C line). A labeled pad contains labeled HAV antibody-2. During detection, the HAV antigen in the sample binds to the labeled antibody-2 to form a complex. Under chromatographic action, the complex moves forward along the nitrocellulose membrane. Upon passing the test zone (T line), it binds to the HAV antibody-1 coated on the membrane, forming a red band in the test zone (T line). The labeled HAV antibody-2 moves to the control zone (C line) and binds to the coated goat anti-mouse IgG polyclonal antibody, forming a red band. Negative samples do not show a red band in the test zone (T line), only in the control zone (C line). Regardless of the presence of HAV viral antigen in the sample, a red band will form in the control zone (C line), serving as a standard for judging whether the chromatographic process is normal.
[0063] (II) Testing process:
[0064] Before testing, allow the stool sample to recover to 15℃~30℃.
[0065] 1. Sample preparation
[0066] (1) Unscrew the dropper of the sample collection filter bottle 10 and take out the sampling rod 3 to prepare to pick up the sample.
[0067] (2) Use sampling stick 3 to pick up stool samples from at least 4 different locations, approximately 125 mg. Then insert the samples into the sample collection filter bottle containing the sample processing solution. If the stool is liquid, use a disposable plastic pipette to draw up the sample and add 3 drops (approximately 120 μL) of the sample into the sample collection filter bottle containing the sample processing solution.
[0068] (3) Tighten the dropper and cap of the sample collection filter bottle.
[0069] (4) Shake well to ensure that the sample and sample processing solution are thoroughly mixed.
[0070] 2. Testing
[0071] (1) Take out the kit, which contains the hepatitis A antigen test strip prepared in step (I). Place the kit on a clean and flat surface. Keep the sample collection filter bottle with the tube 10 facing upwards, unscrew the cap at the top of the sample collection filter bottle, then invert the sample collection filter bottle, gently squeeze the sample collection filter bottle, and vertically add 3 drops of bubble-free sample solution to the reagent application area of the hepatitis A antigen test strip in the kit.
[0072] (2) Wait for the red band to appear, and read the test results in 15 to 20 minutes.
[0073] 3. Interpretation of Test Results
[0074] Standard color chart: The colorimetric range of the standard color chart is between 0 and 10, i.e., L0-L10. The color intensity increases progressively from 0 to 10. When the color intensity of the test line (T line) is between L3 (inclusive) and L10, the test result is positive. When the color intensity of the test line (T line) is between L0 and L3 (exclusive), the test result is negative.
[0075] The test line T of the test strip was compared with the standard color card, and the test results are shown in Table 1.
[0076] Table 1 Sample Detection Results
[0077]
[0078] Based on the test results, adding a first filter does not affect the accuracy and sensitivity of positive and negative tests. For samples treated with activated carbon, the background is cleaner, and the color of the detection line for positive samples is more obvious compared to the background. In particular, for weakly positive samples, the interpretation of positive results is clearer and more definite.
Claims
1. A sample collection filter bottle comprising a bottle body, a drop head, a cap body and a sampling stick, characterized in that, One end of the sampling rod is fixedly connected with the drop head, a hollow chamber is arranged in the sampling rod along the axial direction of the sampling rod, an opening is arranged at the top of the chamber and is communicated with the drop head, the bottom of the chamber is closed to form a sampling part, a first filter and a second filter are arranged on the sampling rod, the first filter is arranged on the outer side of the sampling rod at a position adjacent to the bottom of the drop head, and the second filter is arranged on the outer side of the sampling rod close to one end of the sampling part.
2. The specimen collection filter bottle of claim 1, wherein, A plurality of filter holes are arranged on the outer surface of the first filter, and a plurality of liquid inlet holes which are communicated with the chamber are arranged on the side wall of the sampling rod connected with the first filter.
3. The specimen collection filtering bottle according to claim 1 or 2, characterized by, At least two liquid inlet holes in different directions or heights are arranged on the side wall of the sampling rod.
4. The specimen collection filter bottle of claim 1, wherein, An activated carbon layer is arranged in the first filter and the second filter.
5. The specimen collection filter bottle of claim 1, wherein, The first filter and the second filter are fixedly arranged on the outer wall of the sampling rod and have a circular cross section, and the sample solution can only enter the chamber of the sampling rod through the first filter.
6. The specimen collection filter bottle of claim 4, wherein, The activated carbon layer uses activated carbon cotton mesh or activated carbon filter element.
7. The specimen collection filter bottle of claim 4, wherein, A filter residue layer is further arranged in the first filter, and the filter residue layer is arranged on the outer side of the activated carbon layer.
8. The specimen collection filter bottle of claim 7, wherein, The filter residue layer is made of glass fiber, sponge or absorbent cotton.
9. The specimen collection filtering bottle of claim 1, wherein, The surface of the sampling part is provided with a threaded spiral surface or a plurality of uniformly distributed protrusions.
10. The specimen collection filtering bottle according to claim 1 or 9, characterized by, The surface of the sampling part is wrapped with cotton or sponge.