Stem cell exosome preservation device

Abstract

The utility model relates to a stem cell exosome preservation device. Comprising an outer box body and a sealing cover; an inner container body and a plurality of clamping assemblies are arranged in the outer box body, and a partition plate is arranged between every two adjacent clamping assemblies. A plurality of heat-conducting metal sleeves capable of accommodating test tubes are clamped on each clamping component; the clamping assembly comprises an upper mounting plate and a lower mounting plate, a plurality of elastic clamping units are mounted between the upper mounting plate and the lower mounting plate, and through grooves in one-to-one correspondence with the clamping units are formed in the upper mounting plate; the elastic clamping unit comprises two lower elastic mounting seats fixedly connected to the lower mounting plate, each lower elastic mounting seat is provided with an elastic mounting piece and two lower clamping mechanisms, two upper elastic mounting seats are fixedly connected to the upper mounting plate, and each upper elastic mounting seat is provided with an elastic mounting piece and two upper clamping mechanisms; a pull rod mechanism is further included. The test tubes are stored through the arranged heat conduction metal sleeve, the influence of temperature change on exosomes is reduced, meanwhile, the heat conduction metal sleeve can be clamped and limited, and the situation that the test tubes filled with the exosomes are collided due to shaking during transportation is avoided.

Description

TECHNICAL FIELD

[0001] The utility model belongs to stem cell preservation technical field especially relates to a stem cell exosome storage device. BACKGROUND

[0002] Stem cell exosome research is an important research means of biotechnology, and exosome refers to small membrane vesicles 30-150nm containing complex RNA and protein, and at present, it specifically refers to disc-shaped vesicles with a diameter of 40-100nm;Various cells can secrete exosomes under normal and pathological conditions;It is mainly derived from the multivesicular body formed by intracellular lysosome micro-particle invagination, and is released into the extracellular matrix after fusion of the multivesicular body outer membrane and the cell membrane.

[0003] When the stem cell exosome is stored, it needs to be stored in a special storage device, so that the stem cell exosome can be prevented from being polluted by the external environment. There are some problems in the stem cell exosome storage device at present: firstly, the exosome storage should be carried out in a low-temperature environment as much as possible, and the number of repeated freezing and thawing should be reduced, the traditional stem cell exosome storage technology adopts the mode of storing and taking out a plurality of test tubes at the same time, in the process of taking a test tube, all test tubes need to be exposed to room temperature, which reduces the storage effect of stem cells;In addition, the traditional stem cell exosome storage device has a simple structure, and when storing, the container containing exosomes is directly placed in the storage device, and there is no positioning structure for the container, so that the containers are easy to collide due to shaking during moving and transporting, which affects the exosomes. Therefore, it is urgent to design a stem cell exosome storage device to solve the above problems. CONTENT OF THE UTILITY MODEL

[0004] The utility model discloses a stem cell exosome storage device with reasonable structure design to solve the technical problems in the prior art. The utility model discloses a heat conduction metal sleeve storage test tube is arranged, reduces the influence of temperature change to exosome, can clamp and limit the heat conduction metal sleeve simultaneously, avoids the test tube containing exosome from colliding due to shaking during transportation.

[0005] The utility model discloses a stem cell exosome storage device, which comprises an outer box body, a window is formed on the top of the outer box body, and a sealing cover is arranged at the window; an inner container is arranged in the outer box body, a plurality of groups of clamping assemblies are arranged in the inner container in parallel, and a partition plate is arranged between every two adjacent groups of clamping assemblies; a plurality of heat-conducting metal sleeves capable of accommodating test tubes are clamped on each clamping assembly; the clamping assembly comprises an upper mounting plate and a lower mounting plate which are installed in the inner container, a plurality of elastic clamping units are installed between the upper mounting plate and the lower mounting plate in parallel, and a through slot for penetrating the heat-conducting metal sleeve is formed in the upper mounting plate and corresponds to the plurality of clamping units one by one; the elastic clamping unit comprises two oppositely arranged lower elastic mounting seats which are fixedly connected to the lower mounting plate, an elastic mounting piece is installed on each lower elastic mounting seat, and two oppositely arranged lower clamping mechanisms are installed on the elastic mounting piece; two oppositely arranged upper elastic mounting seats are fixedly connected to the upper mounting plate, an elastic mounting piece is installed on each upper elastic mounting seat, and two oppositely arranged upper clamping mechanisms are installed on the elastic mounting piece; a pull rod mechanism is installed on the lower mounting plate and is used for synchronously pushing the two upper clamping mechanisms and the two lower clamping mechanisms outward.

[0006] The stem cell exosome storage device provided by the utility model has the advantages that the heat-conducting metal sleeve is arranged, the test tube can be inserted into the heat-conducting metal sleeve, the test tube for storing stem cell exosomes can be isolated and operated, the influence of temperature change in the outer box body on the test tube after the sealing cover installed on the outer box body is opened is small, the test tube for storing stem cell exosomes in the heat-conducting metal sleeve will not change due to the opening of the sealing cover, the clamping assembly is arranged, the heat-conducting metal sleeve can be clamped and positioned, the pull rod mechanism only needs to be pulled upward, the operation process is simple, and the storage device is prevented from colliding due to shaking during movement and transportation, so that the exosomes are not affected.

[0007] Preferably, the lower clamping mechanism comprises an adapter connected to the inner end of the corresponding elastic mounting piece, the adapter is provided with a lower clamping jaw matched with the outer wall of the heat-conducting metal sleeve, and the lower linkage block is in frictional contact with the pull rod mechanism and connected to the adapter, and a lower pushing guide groove is formed in the inner side surface of the lower linkage block and gradually approaches the pull rod mechanism from bottom to top.

[0008] Preferably, the upper clamping mechanism comprises an upper clamping jaw connected to the inner end of the elastic mounting piece and matched with the outer wall of the heat-conducting metal sleeve, and the upper linkage block is connected to the upper clamping jaw through a plate piece, the upper linkage block is in frictional contact with the pull rod mechanism, and an upper pushing guide groove is formed in the inner side surface of the upper linkage block and gradually approaches the pull rod mechanism from bottom to top.

[0009] Preferably, the pull rod mechanism comprises a longitudinally arranged mounting guide sleeve fixed on the lower mounting plate, a driving pull rod slidingly inserted in the mounting guide sleeve, a compression spring arranged in top tight contact with the mounting guide sleeve and the driving pull rod, a transversely arranged lower pushing cross rod fixed on the driving pull rod, two ends of the lower pushing cross rod movably penetrating in two lower pushing guide slots, a transversely arranged upper pushing cross rod fixed on the driving pull rod, and two ends of the upper pushing cross rod movably penetrating in two upper pushing guide slots.

[0010] Preferably, the elastic mounting member comprises a spring guide rod arranged transversely and slidingly connected with the corresponding elastic mounting seat, a compression spring sleeved on the spring guide rod, and the compression spring being arranged between and in top tight contact with the corresponding clamping jaw and the elastic mounting seat.

[0011] Preferably, the clamping assembly further comprises a plurality of supporting sleeves respectively corresponding to the plurality of clamping units and mounted on the lower mounting plate, and a rubber pad mounted in each supporting sleeve.

[0012] Preferably, a longitudinally arranged air outlet plate is fixed in the inner container body to divide the inner cavity into a storage cavity and a blowing cavity, a plurality of air holes are formed in the air outlet plate, and a fan is arranged in the blowing cavity of the inner container body, and an air outlet of the fan is in communication with the storage cavity. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 is the front view structural schematic diagram of the utility model;

[0014] Figure 2 is the three-dimensional structure schematic diagram of the clamping assembly in the utility model;

[0015] Figure 3 is the three-dimensional structure schematic diagram of the clamping unit in the utility model, the front view angle;

[0016] Figure 4 is the three-dimensional structure schematic diagram of the clamping unit in the utility model, the rear view angle.

[0017] In the drawing: 1, outer box body; 2, inner container body; 3, air outlet plate; 4, fan; 5, clamping assembly; 5-1, pull rod mechanism; 5-1-1, mounting guide sleeve; 5-1-2, driving pull rod; 5-1-3, upper pushing cross rod; 5-1-4, lower pushing cross rod; 5-1-5, pull ring; 5-2, supporting sleeve; 5-3, lower elastic mounting seat; 5-4, elastic mounting member; 5-5, lower clamping jaw; 5-6, lower linkage block; 5-6-1, lower pushing guide slot; 5-7, upper elastic mounting seat; 5-8, upper linkage block; 5-8-1, upper pushing guide slot; 5-9, upper clamping jaw; 5-10, upper mounting plate; 5-11, lower mounting plate; 6, heat-conducting metal sleeve; 7, partition plate; 8, motor cover; 9, sealing cover. DETAILED DESCRIPTION

[0018] In order to further understand the application content, characteristics and effects of the present application, the following embodiments are described in detail as follows:

[0019] Please see Figure 1 The stem cell exosome storage device comprises an outer box body 1, a window is formed at the top of the outer box body 1, and a sealing cover 9 is arranged at the window; an inner container body 2 is arranged in the outer box body 1, a plurality of groups of clamping assemblies 5 are arranged in the inner container body 2, and a partition plate 7 is arranged between every two adjacent groups of clamping assemblies 5; a plurality of heat-conducting metal sleeves 6 capable of accommodating test tubes are clamped on each clamping assembly 5.

[0020] As shown in Figure 2 The clamping assembly 5 comprises an upper mounting plate 5-10 and a lower mounting plate 5-11 mounted in the inner container body 2, a plurality of elastic clamping units are arranged in parallel between the upper mounting plate 5-10 and the lower mounting plate 5-11, and a through slot for penetrating the heat-conducting metal sleeve 6 is formed in the upper mounting plate 5-10 and corresponds to each clamping unit.

[0021] As shown in Figure 3 and Figure 4 The elastic clamping unit comprises two oppositely arranged lower elastic mounting seats 5-3 fixedly connected to the lower mounting plate 5-11, an elastic mounting piece 5-4 is mounted on each lower elastic mounting seat 5-3, and two oppositely arranged lower clamping mechanisms are mounted on the elastic mounting piece 5-4, two oppositely arranged upper elastic mounting seats 5-7 are fixedly connected to the upper mounting plate 5-10, an elastic mounting piece 5-4 is mounted on each upper elastic mounting seat 5-7, and two oppositely arranged upper clamping mechanisms are mounted on the elastic mounting piece 5-4; a pull rod mechanism 5-1 mounted on the lower mounting plate 5-11 is used for synchronously pushing the two upper clamping mechanisms and the two lower clamping mechanisms outward.

[0022] The lower clamping mechanism comprises a connecting piece connected to the inner end of the corresponding elastic mounting piece 5-4, and a lower clamping jaw 5-5 adapted to the outer wall of the heat-conducting metal sleeve 6 is mounted on the connecting piece; a lower linkage block 5-6 in frictional contact with the pull rod mechanism 5-1 and connected to the connecting piece is further included, and a lower pushing guide groove 5-6-1 gradually approaching the pull rod mechanism 5-1 from bottom to top is formed on the inner side surface of the lower linkage block 5-6. The cross section of the lower clamping jaw 5-5 and the upper clamping jaw 5-9 is arc-shaped, and a plurality of anti-skid grooves are formed in the inner walls of the lower clamping jaw 5-5 and the upper clamping jaw 5-9.

[0023] The upper clamping mechanism comprises an upper clamping jaw 5-9 connected with the inner end of the elastic mounting member 5-4 and matched with the outer wall of the heat-conducting metal sleeve 6, and an upper linkage block 5-8 connected with the upper clamping jaw 5-9 through a plate member, the upper linkage block 5-8 being in frictional contact with the pull rod mechanism 5-1, and an upper pushing guide groove 5-8-1 being formed in the inner side surface of the upper linkage block 5-8 and gradually approaching the pull rod mechanism 5-1 from bottom to top.

[0024] The elastic mounting member 5-4 comprises spring guide rods arranged transversely and slidably connected with corresponding elastic mounting seats, and compression springs are sleeved on the spring guide rods and in abutting contact with the corresponding clamping jaws and elastic mounting seats. Limiting plate members are mounted on the outer ends of the spring guide rods.

[0025] The pull rod mechanism 5-1 comprises a longitudinally arranged mounting guide sleeve 5-1-1 fixed on a lower mounting plate 5-11, a driving pull rod 5-1-2 slidably inserted in the mounting guide sleeve 5-1-1, a compression spring arranged in abutting contact between the mounting guide sleeve 5-1-1 and the driving pull rod 5-1-2, a transversely arranged lower pushing cross rod 5-1-4 fixed on the driving pull rod 5-1-2, both ends of the lower pushing cross rod 5-1-4 being movably inserted in two lower pushing guide grooves 5-6-1, a transversely arranged upper pushing cross rod 5-1-3 fixed on the driving pull rod 5-1-2, both ends of the upper pushing cross rod 5-1-3 being movably inserted in two upper pushing guide grooves 5-8-1, and a pull ring 5-1-5 mounted on the upper end of the driving pull rod 5-1-2.

[0026] The clamping assembly 5 further comprises a plurality of supporting sleeves 5-2 mounted on the lower mounting plate 5-11 and corresponding to the plurality of clamping units respectively, and a rubber pad is mounted in each supporting sleeve 5-2.

[0027] The inner container body 2 is made of heat-insulating material, a longitudinally arranged air outlet plate 3 is fixedly connected in the inner container body 2 to divide the inner cavity of the inner container body 2 into a storage cavity and a blowing cavity, a plurality of air holes are formed in the air outlet plate 3, and a fan 4 is arranged in the blowing cavity of the inner container body 2, with the air outlet of the fan 4 being in communication with the storage cavity. A motor cover 8 is mounted on the outer box body 1 and covers the fan 4. In actual use, ice bags or other cooling substances can be arranged in the inner container body 2.

[0028] Working principle:

[0029] In the actual working process, when the test tube needs to be taken out and placed, after the outer box body 1 is opened, the corresponding pull ring 5-1-5 is pulled upward, and then the driving pull rod 5-1-2 and the upper pushing cross rod 5-1-3 and the lower pushing cross rod 5-1-4 installed thereon are pulled upward to move upward, the upward moving upper pushing cross rod 5-1-3 and the lower pushing cross rod 5-1-4 can push the two upper linkage blocks 5-8 and the two lower linkage blocks 5-6 outward, and then push the two upper clamping jaws 5-9 and the two lower clamping jaws 5-5 outward to move outward, so that the distance between the two upper clamping jaws 5-9 and the two lower clamping jaws 5-5 is increased to be able to put in the heat conducting metal sleeve 6, and then the staff inserts the test tube from top to bottom; when the two upper clamping jaws 5-9 and the two lower clamping jaws 5-5 move outward, the compression spring on the compression elastic mounting piece 5-4 is compressed, and after the staff releases the pulling of the pull rod mechanism 5-1, the upper pushing cross rod 5-1-3 and the lower pushing cross rod 5-1-4 move downward under the action of their own gravity, the two upper clamping jaws 5-9 and the two lower clamping jaws 5-5 move towards each other under the action of the compression spring to reset, when the heat conducting metal sleeve 6 is arranged between the two upper clamping jaws 5-9 and the two lower clamping jaws 5-5, the two upper clamping jaws 5-9 and the two lower clamping jaws 5-5 can realize the clamping and positioning operation of the culture vessel under the action of the compression spring; after the sealing cover 9 of the outer box body 1 is opened, the temperature change in the inner part of the outer box body 1 has little effect on the test tube, so that the test tube for storing stem cell exosomes in the heat conducting metal sleeve 6 will not change due to the opening of the sealing cover 9 of the outer box body 1.

Claims

1. A stem cell exosome preservation device, characterized in that: The device includes an outer casing (1), with a window at the top and a sealing cover (9) at the window; an inner liner (2) is provided inside the outer casing (1), and multiple sets of clamping assemblies (5) are arranged in parallel inside the inner liner (2), with partitions (7) between adjacent sets of clamping assemblies (5); several heat-conducting metal sleeves (6) capable of accommodating test tubes are clamped on each clamping assembly (5); the clamping assembly (5) includes an upper mounting plate (5-10) and a lower mounting plate (5-11) installed inside the inner liner (2), and several elastic clamping units are arranged in parallel between the upper mounting plate (5-10) and the lower mounting plate (5-11), with a corresponding opening on the upper mounting plate (5-10) for inserting guide tubes. The through slot of the hot metal sleeve (6); the elastic clamping unit includes two oppositely arranged lower elastic mounting seats (5-3) fixedly connected to the lower mounting plate (5-11), each lower elastic mounting seat (5-3) is equipped with an elastic mounting member (5-4), and two oppositely arranged lower clamping mechanisms are installed through the elastic mounting member (5-4); two oppositely arranged upper elastic mounting seats (5-7) are fixedly connected to the upper mounting plate (5-10), each upper elastic mounting seat (5-7) is equipped with an elastic mounting member (5-4), and two oppositely arranged upper clamping mechanisms are installed through the elastic mounting member (5-4); it also includes a pull rod mechanism (5-1) installed on the lower mounting plate (5-11) for synchronously pushing the two upper clamping mechanisms and the two lower clamping mechanisms outward.

2. The stem cell exosome preservation device as described in claim 1, characterized in that: The lower clamping mechanism includes a connector that connects to the inner end of the corresponding elastic mounting member (5-4), and a lower clamping claw (5-5) that is adapted to the outer wall of the heat-conducting metal sleeve (6) is mounted on the connector; it also includes a lower linkage block (5-6) that is in frictional contact with the pull rod mechanism (5-1) and connected to the connector, and a lower push guide groove (5-6-1) that gradually approaches the pull rod mechanism (5-1) from bottom to top is provided on the inner side of the lower linkage block (5-6).

3. The stem cell exosome preservation device as described in claim 2, characterized in that: The upper clamping mechanism includes an upper jaw (5-9) that is connected to the inner end of the elastic mounting member (5-4) and adapted to the outer wall of the heat-conducting metal sleeve (6), and an upper linkage block (5-8) that is connected to the upper jaw (5-9) through a plate. The upper linkage block (5-8) is in frictional contact with the pull rod mechanism (5-1). An upper push guide groove (5-8-1) is provided on the inner side of the upper linkage block (5-8) that gradually approaches the pull rod mechanism (5-1) from bottom to top.

4. The stem cell exosome preservation device as described in claim 3, characterized in that: The lever mechanism (5-1) includes a longitudinally arranged mounting guide sleeve (5-1-1) fixed to the lower mounting plate (5-11), a drive lever (5-1-2) slidably inserted in the mounting guide sleeve (5-1-1), a compression spring that is in abutting contact between the mounting guide sleeve (5-1-1) and the drive lever (5-1-2), a transversely arranged lower push crossbar (5-1-4) fixed to the drive lever (5-1-2), the two ends of the lower push crossbar (5-1-4) movably passing through two lower push guide grooves (5-6-1), and also includes a transversely arranged upper push crossbar (5-1-3) fixed to the drive lever (5-1-2), the two ends of the upper push crossbar (5-1-3) movably passing through two upper push guide grooves (5-8-1); and also includes a pull ring (5-1-5) installed at the upper end of the drive lever (5-1-2).

5. The stem cell exosome preservation device as described in claim 1, characterized in that: The elastic mounting component (5-4) includes a spring guide rod arranged laterally and slidably connected to the corresponding elastic mounting seat. A compression spring is sleeved on the spring guide rod, and the compression spring is located between the corresponding gripper and the elastic mounting seat and is in abutting contact with both.

6. The stem cell exosome preservation device as described in claim 1, characterized in that: The clamping assembly (5) also includes multiple support sleeves (5-2) installed on the lower mounting plate (5-11), each corresponding to a multiple clamping unit, and each support sleeve (5-2) is equipped with a rubber pad.

7. The stem cell exosome preservation device as described in claim 1, characterized in that: A longitudinally arranged air outlet plate (3) is fixed inside the inner liner (2) to divide its inner cavity into a storage cavity and a blower cavity. Several ventilation holes are opened on the air outlet plate (3). A fan (4) is installed in the blower cavity of the inner liner (2), and the air outlet of the fan (4) is connected to the storage cavity.

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