Automatic dilution device for immunodetection sample

Abstract

The utility model relates to the field of immunodetection, and discloses an automatic immunodetection sample diluting device which comprises a square box, a first motor is fixedly connected in the square box, a driving end of the first motor is fixedly connected with a transmission column, and the top of the transmission column is fixedly connected with a push rod. A first connecting rod is fixedly connected to the right side of the interior of the square box, a ratchet wheel is rotatably connected to the outer wall of the first connecting rod, a first rotating disc is fixedly connected to the top of the ratchet wheel, a plurality of first test tubes are fixedly connected to the interior of the first rotating disc, and a belt is connected to the exterior of a transmission column in a coupled mode. The rotating disc rotates for dilution through the output of the motor, and the rotating dilution enables a sample and a diluent to form a complex flowing mode in the reaction container under the combined action of centrifugal force and shearing force, so that more sufficient and more uniform mixing is realized.

Description

Technical Field

[0001] This utility model relates to the field of immunoassay, and in particular to an automatic dilution device for immunoassay samples. Background Technology

[0002] An automated immunoassay sample dilution device is a piece of equipment used in the field of immunoassay that can automatically perform sample dilution. The device is programmed via a control module. The sample processing module's aspiration device draws a predetermined volume of raw sample from the sample container, while the diluent supply module dispenses the corresponding volume of diluent into the sample-containing container according to a preset dilution ratio. Then, the mixing module operates, using methods such as rotation, stirring, or ultrasound to thoroughly mix the sample and diluent, achieving uniform dilution to meet the sample concentration requirements of subsequent immunoassay methods.

[0003] The automated immunoassay sample dilution device rotates to ensure the accuracy and uniformity of dilution. Rotation accelerates the diffusion and convection of various components in the sample and diluent, allowing substances required for the immunoassay to interact more quickly. This helps shorten detection time, improve overall detection efficiency, reduce bubble generation, enhance detection accuracy, reduce the risk of cross-contamination, and facilitate automated operation. Rotational dilution enables precise mixing of the sample and diluent, resulting in a more accurate dilution ratio and thus improved detection precision. Rotational dilution is typically performed in a closed reaction vessel, reducing sample contact with the external environment, lowering the risk of cross-contamination, minimizing human error, and facilitating the processing of multiple samples.

[0004] In automated immunoassay sample dilution devices, uneven mixing can lead to insufficient mixing of the sample and the diluent, causing localized aggregation of components and resulting in uneven concentrations after dilution. This uneven mixing directly increases the error in the test results, leading to inconsistent results, reduced reliability and accuracy of the test data, and decreased work efficiency. For large-scale immunoassay samples, non-rotational dilution methods have low processing efficiency and cannot meet the needs of high-throughput testing. Therefore, an automated immunoassay sample dilution device is proposed to solve the above problems. Utility Model Content

[0005] To overcome the above shortcomings, this utility model provides an automatic dilution device for immunoassay samples, which aims to improve the problems of uneven mixing, local aggregation, uneven concentration, and increased error in the prior art.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] An automated dilution device for immunoassay samples includes a square box. A motor is fixedly connected inside the square box, and a transmission column is fixedly connected to the drive end of the motor. A push rod is fixedly connected to the top of the transmission column. A connecting rod is fixedly connected to the right side of the square box, and a ratchet is rotatably connected to the outer wall of the connecting rod. A turntable is fixedly connected to the top of the ratchet, and multiple test tubes are fixedly connected inside the turntable. A belt is coupled to the outside of the transmission column. A connecting rod is rotatably connected to the left side of the square box, and a limit wheel is fixedly connected to the outer wall of the connecting rod. A turntable is fixedly connected to the top of the connecting rod, and multiple test tubes are fixedly connected inside the turntable. A fixing plate is fixedly connected to the inner wall of the square box, and a lifting mechanism assembly is fixedly connected to the top of the fixing plate.

[0008] As a further description of the above technical solution:

[0009] The lifting mechanism comprises two hydraulic cylinders, the bottoms of which are fixedly connected to the top left and right sides of the fixed plate one, respectively. A rack is fixedly connected to the top of each hydraulic cylinder, and a guide rail is fixedly connected to the top of the rack. A fixed plate two is slidably connected to the top of the guide rail. A hollow rectangular body is fixedly connected to the top of the fixed plate two, and a motor two is fixedly connected inside the hollow rectangular body. A bevel gear one is fixedly connected to the drive end of the motor two. Connecting rods three are rotatably connected to the left and right sides of the interior of the fixed plate two, and bevel gears two are fixedly connected to the top of the connecting rods three. Rollers are fixedly connected to the outer walls of both connecting rods three, and a rotating gear is fixedly connected to the bottom of the connecting rods three.

[0010] As a further description of the above technical solution:

[0011] The inside of the square box is rotatably connected to a glass window, and the top right side of the second fixed plate is fixedly connected to a third fixed plate. The inside left and right sides of the square box are both fixedly connected to fixed rods.

[0012] As a further description of the above technical solution:

[0013] The outer wall of turntable one is rotatably connected to the inner wall of fixed plate one, and the outer wall of turntable two is rotatably connected to the inner wall of fixed plate one.

[0014] As a further description of the above technical solution:

[0015] The outer sides of the first bevel gear and the second bevel gear are meshed with each other; the outer sides of the rotating gear and the rack are meshed with each other; and the outer sides of the ratchet and the push rod are meshed with each other.

[0016] As a further description of the above technical solution:

[0017] The outer walls of both rollers are rotatably connected to the inside of the guide rail, and the left and right sides of the rack are slidably connected to the inside of the square box;

[0018] As a further description of the above technical solution:

[0019] The belt is internally coupled to the outside of the limiting wheel and the inside of the two fixing rods on the left and right sides;

[0020] As a further description of the above technical solution:

[0021] The right side of the fixing plate three is fixedly connected to the left side of the hollow rectangular body, and a sample needle is fixedly connected to the bottom of the fixing plate three.

[0022] This utility model has the following beneficial effects:

[0023] 1. In this invention, dilution is achieved by rotating a turntable through the output of a motor. Rotational dilution allows the sample and diluent to form a complex flow pattern within the reaction vessel under the combined action of centrifugal force and shear force, thereby achieving more thorough and uniform mixing. Compared with traditional stirring or shaking methods, rotational dilution can avoid localized incomplete mixing, ensuring uniform sample concentration after dilution and improving the accuracy and reliability of test results. By precisely controlling parameters such as rotation speed, time, and angle, automated processing of different types of samples and different dilution requirements can be achieved, improving work efficiency and reducing the impact of human factors on experimental results.

[0024] 2. In this utility model, multi-directional movement is achieved through the cooperation of hydraulic cylinders and motors, which can quickly switch between samples and reagents, thereby improving the overall detection efficiency. Multiple samples can be processed simultaneously, and samples can be moved flexibly between different samples. Samples can be easily retrieved from the sample storage location and then moved to the designated location for dilution operations, realizing parallel dilution and detection of samples. The multi-directional movement function allows the automatic dilution device to be precisely aligned with the interfaces of these devices or the sample placement location, realizing automated sample transfer and processing, avoiding alignment deviations caused by manual operation or fixed positions, improving the versatility and adaptability of the device, reducing manual intervention, reducing human error, and improving detection efficiency and quality control level. Attached Figure Description

[0025] Figure 1 This is a three-dimensional schematic diagram of an automatic dilution device for immunoassay samples proposed in this utility model;

[0026] Figure 2 This is a schematic diagram of the hydraulic cylinder of an automatic dilution device for immunoassay samples proposed in this utility model;

[0027] Figure 3 This is a schematic diagram of the guide rail structure of an automatic dilution device for immunoassay samples proposed in this utility model;

[0028] Figure 4 This is a schematic diagram of the rotating disk of an automatic dilution device for immunoassay samples proposed in this utility model.

[0029] Legend:

[0030] 1. Square box; 2. Motor 1; 3. Transmission column; 4. Push rod; 5. Connecting rod 1; 6. Ratchet; 7. Turntable 1; 8. Test tube 1; 9. Belt; 10. Connecting rod 2; 11. Limiting wheel; 12. Turntable 2; 13. Test tube 2; 14. Fixing plate 1; 15. Hydraulic cylinder; 16. Rack; 17. Guide rail; 18. Fixing plate 2; 19. Hollow rectangular body; 20. Motor 2; 21. Bevel gear 1; 22. Connecting rod 3; 23. Bevel gear 2; 24. Roller; 25. Rotating gear; 26. Fixing plate 3; 27. Sample needle; 28. Glass window; 29. ​​Fixing rod. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] Reference Figures 1 to 3 This utility model provides an embodiment of an automatic dilution device for immunoassay samples, comprising a square box 1. The square box 1 provides a stable mounting platform for the internal precision components and also serves a protective function. A motor 2 is fixedly connected inside the square box 1. The square box 1 provides stability for the motor 2, enabling the motor 2 to output more stably. A transmission column 3 is fixedly connected to the drive end of the motor 2. When the motor 2 is running, the rotational motion of the drive end will drive the transmission column 3 to rotate. A push rod 4 is fixedly connected to the top of the transmission column 3. As the transmission column 3 rotates, the push rod 4 will make a circular motion around the axis of the transmission column 3, and its motion trajectory is circular.

[0033] A connecting rod 5 is fixedly connected to the right side of the inside of the square box 1. The square box 1 provides stability for the connecting rod 5, ensuring stable output for subsequent components. A ratchet 6 is rotatably connected to the outer wall of the connecting rod 5. A turntable 7 is fixedly connected to the top of the ratchet 6. The ratchet 6 can rotate outside the connecting rod 5. When the push rod 4 moves with the transmission column 3, it will push the ratchet 6 to rotate, which will drive the turntable 7 connected to the top to rotate synchronously. Multiple test tubes 8 are fixedly connected inside the turntable 7. The test tubes 8 are used to hold the immunoassay samples to be diluted. When the turntable 7 rotates intermittently, the test tubes inside will also rotate, making it easier for the sample needle 27 to extract the samples. A belt 9 is coupled to the outside of the transmission column 3.

[0034] The transmission column 3 is rotated by a motor, and the belt 9 connected to the outside of the transmission column 3 rotates accordingly, driving the subsequent components. The left side of the inside of the square box 1 is rotatably connected to the connecting rod 2 10. The square box 1 provides stability to the connecting rod 2 10, making it more stable when rotating. The outer wall of the connecting rod 2 10 is fixedly connected to the limiting wheel 11, which is connected to the belt 9. When the belt 9 rotates, it will drive the limiting wheel 11 and the connecting rod 2 10 to rotate. The top of the connecting rod 2 10 is fixedly connected to the turntable 2 12. Multiple test tubes 2 13 are fixedly connected inside the turntable 2 12. When the connecting rod 2 10 rotates, it drives the turntable 2 12 to rotate synchronously, driving the diluted immunoassay sample in the test tube 2 13 to rotate and dilute. The inner wall of the square box 1 is fixedly connected to the fixing plate 14. The fixing plate 14 provides protection for the transmission components at the bottom of the square box 1, preventing the immunoassay sample from spilling onto the motor 2. The top of the fixing plate 14 is fixedly connected to the components of the lifting mechanism, and the fixing plate 14 provides a stable installation platform.

[0035] Reference Figure 3 and Figure 4 The lifting mechanism comprises two hydraulic cylinders 15, the bottoms of which are fixedly connected to the top left and right sides of the fixed plate 14. The hydraulic cylinders 15 provide stable and reliable power support for the entire lifting mechanism. A rack 16 is fixedly connected to the top of each hydraulic cylinder 15, providing stability to the rack 16 and achieving synchronous lifting. A guide rail 17 is fixedly connected to the top of the rack 16, and a fixed plate 18 is slidably connected to the top of the guide rail 17, providing stable guidance for the movement of subsequent components and ensuring the accuracy and stability of the vertical movement of the fixed plate 18. A hollow rectangular body 19 is fixedly connected to the top of the fixed plate 18, providing stability and a mounting platform for the hollow rectangular body 19. A motor 20 is fixedly connected inside the hollow rectangular body 19, providing stability to the motor 20 and enabling the motor 20 to output power stably.

[0036] The drive end of motor 20 is fixedly connected to bevel gear 21. When motor 20 operates, bevel gear 21 can rotate and transmit motion to other components. The left and right sides of the inside of fixed plate 218 are rotatably connected to connecting rod 32. The top of connecting rod 32 is fixedly connected to bevel gear 23. When bevel gear 21 rotates under the drive of motor 20, due to the special tooth structure between bevel gears, the horizontal rotational motion can be converted into the vertical rotational motion, thereby driving connecting rod 32 to rotate around its axis. Rollers 24 are fixedly connected to the outer walls of both connecting rods 32. Rotating gear 25 is fixedly connected to the bottom of connecting rod 32. When connecting rod 32 rotates, it will also drive rollers 24 and rotating gear 25 to rotate, achieving the effect of parallel movement.

[0037] Reference Figures 2 to 4 The interior of the square box 1 is rotatably connected to a glass window 28, through which the interior of the square box 1 can be observed. The top right side of the fixed plate 2 18 is fixedly connected to a fixed plate 3 26. The fixed plate 2 18 provides stability for the fixed plate 3 26. The left and right sides of the interior of the square box 1 are fixedly connected to fixed rods 29. The fixed rods 29 are fixed inside the square box 1 and provide a limiting and fixing function for the glass window 28. The outer wall of the turntable 1 7 is rotatably connected to the inner wall of the fixed plate 14. The outer wall of the turntable 2 12 is rotatably connected to the inner wall of the fixed plate 14. The fixed plate 14 has turntable 1 7 and turntable 2 12 fixed inside to prevent them from shaking during rotation and to provide them with stability.

[0038] The outer surfaces of bevel gear 21 and bevel gear 23 are meshed together. Bevel gear 21 transmits power to bevel gear 23 through the meshing relationship between them. The outer surfaces of rotating gear 25 and rack 16 are meshed together. Rotating gear 25 moves parallel inside rack 16 through power transmission from motor 20. The outer surfaces of ratchet 6 and push rod 4 are meshed together. Through transmission from motor 2, transmission column 3 drives push rod 4 to rotate ratchet 6 intermittently. The outer walls of two rollers 24 are rotatably connected to the inside of guide rail 17. Rollers 24 can move parallel inside guide rail 17 under the drive of rotating gear 25. The left and right sides of rack 16 are slidably connected to the inside of square box 1. The inside of square box 1 is equipped with slide rails so that the left and right sides of rack 16 can slide within them, making the lifting and lowering movement more stable.

[0039] The belt 9 is internally coupled to the outside of the limiting wheel 11. When the belt 9 rotates, the limiting wheel 11 will also rotate synchronously. The bottoms of the two fixing rods 29 are respectively rotatably connected to the left and right sides of the inside of the glass window 28. The fixing rods 29 provide stable support and a certain degree of freedom of movement for the glass window 28. The right side of the fixing plate 3 26 is fixedly connected to the left side of the hollow rectangular body 19. The fixing plate 3 26 provides stability for the hollow rectangular body 19. The bottom of the fixing plate 3 26 is fixedly connected to the sample needle 27. The fixing plate 3 26 provides stability for the sample needle, so that it can output stably during operation.

[0040] Working principle: After motor 2 starts, its drive end drives transmission column 3 to rotate. Transmission column 3 drives limit wheel 11 to rotate via belt 9, causing connecting rod 10 and turntable 12 to rotate synchronously. Test tube 13 inside turntable 12 rotates accordingly, which can be used to carry and transport samples or reagents. At the same time, push rod 4 at the top of transmission column 3 rotates with transmission column 3. Since push rod 4 meshes with ratchet 6, push rod 4 will push ratchet 6 to rotate intermittently around connecting rod 5, thereby driving turntable 7 to rotate intermittently. Multiple test tubes 8 inside turntable 7 also realize intermittent position changes, which can sequentially send different samples or reagents to designated positions.

[0041] A guide rail 17 is connected to the top of the rack 16, and a hollow rectangular body 19 and a fixed plate 26 are connected to the top of the second fixed plate 18. A motor 20 is connected inside the hollow rectangular body 19. The motor 20 outputs power to rotate the first bevel gear 21, which transmits power to the second bevel gear 23. The connecting rod 22 connected inside the second bevel gear 23 rotates, which drives the roller 24 and the rotating gear 25 to rotate synchronously. When the rotating gear 25 rotates through the output of the second motor 20, it moves left and right in the rack 16, causing the two rollers 24 connected to the second fixed plate 18 to move. The two move synchronously, and the output of the hydraulic cylinder 15 drives the rack 16, which in turn drives the components at the top of the rack 16 to extend and retract, achieving the effect of multi-directional movement.

[0042] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An automatic dilution device for immunological detection of samples, comprising a square box (1), characterized in that: A motor (2) is fixedly connected inside the square box (1). A transmission column (3) is fixedly connected to the drive end of the motor (2). A push rod (4) is fixedly connected to the top of the transmission column (3). A connecting rod (5) is fixedly connected to the right side inside the square box (1). A ratchet (6) is rotatably connected to the outer wall of the connecting rod (5). A turntable (7) is fixedly connected to the top of the ratchet (6). Multiple test tubes (8) are fixedly connected inside the turntable (7). The transmission column (3) The external coupling connection is a belt (9), and the inside left side of the square box (1) is rotatably connected to a connecting rod two (10). The outer wall of the connecting rod two (10) is fixedly connected to a limit wheel (11). The top of the connecting rod two (10) is fixedly connected to a turntable two (12). The inside of the turntable two (12) is fixedly connected to multiple test tubes two (13). The inner wall of the square box (1) is fixedly connected to a fixing plate one (14). The top of the fixing plate one (14) is fixedly connected to a component for lifting mechanism.

2. The automatic dilution device for immunoassay samples according to claim 1, characterized in that: The lifting mechanism comprises two hydraulic cylinders (15), the bottoms of which are fixedly connected to the top left and right sides of the first fixed plate (14), respectively. A rack (16) is fixedly connected to the top of each of the two hydraulic cylinders (15). A guide rail (17) is fixedly connected to the top of the rack (16). A second fixed plate (18) is slidably connected to the top of the guide rail (17). A hollow rectangular body (19) is fixedly connected to the top of the second fixed plate (18). A motor 2 (20) is fixedly connected inside the centrifugal body (19). A bevel gear 1 (21) is fixedly connected to the drive end of the motor 2 (20). A connecting rod 3 (22) is rotatably connected to both the left and right sides inside the fixed plate 2 (18). A bevel gear 2 (23) is fixedly connected to the top of the connecting rod 3 (22). Rollers (24) are fixedly connected to the outer walls of the two connecting rods 3 (22). A rotating gear (25) is fixedly connected to the bottom of the connecting rod 3 (22).

3. The automatic dilution device for immunoassay samples according to claim 2, characterized in that: The inside of the square box (1) is rotatably connected to a glass window (28), and the top right side of the second fixing plate (18) is fixedly connected to a third fixing plate (26). The inside of the square box (1) is fixedly connected to both the left and right sides with fixing rods (29).

4. The automatic dilution device for immunoassay samples according to claim 1, characterized in that: The outer wall of turntable one (7) is rotatably connected to the inner wall of fixed plate one (14), and the outer wall of turntable two (12) is rotatably connected to the inner wall of fixed plate one (14).

5. The automatic dilution device for immunoassay samples according to claim 2, characterized in that: The outer sides of the first bevel gear (21) and the second bevel gear (23) are meshed with each other, the outer sides of the rotating gear (25) and the rack (16) are meshed with each other, and the outer sides of the ratchet (6) and the push rod (4) are meshed with each other.

6. The automatic dilution device for immunoassay samples according to claim 2, characterized in that: The outer walls of the two rollers (24) are rotatably connected to the inside of the guide rail (17), and the left and right sides of the rack (16) are slidably connected to the inside of the square box (1).

7. The automatic dilution device for immunoassay samples according to claim 3, characterized in that: The belt (9) is internally coupled to the outside of the limiting wheel (11), and the bottoms of the two fixing rods (29) are respectively rotatably connected to the left and right sides inside the glass window (28).

8. The automatic dilution device for immunoassay samples according to claim 3, characterized in that: The right side of the fixing plate three (26) is fixedly connected to the left side of the hollow rectangular body (19), and the bottom of the fixing plate three (26) is fixedly connected to the sample needle (27).

Images