Sample pretreatment device for gene detection of 10-50 g food sample
By designing multiple independent pretreatment units and a rotary cutting device, the problems of capacity limitation, personalized processing, and cross-contamination in existing sample pretreatment devices have been solved, achieving efficient and accurate gene detection of food samples.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-16
- Publication Date
- 2026-03-24
AI Technical Summary
Existing sample pretreatment devices have capacity limitations when processing 10-50g food samples, cannot personalize the processing of different sample types, and are prone to generating high temperatures that affect DNA integrity and cause cross-contamination, leading to inaccurate test results and safety risks.
A sample pretreatment device comprising multiple independent pretreatment units was designed. Each unit is equipped with a 50ml pretreatment container and a rotary cutting unit, supporting sample processing of 10-50g. It employs independent processing and lifting platforms to prevent cross-contamination, and optimizes the processing process through a guiding stabilization platform and heat dissipation structure.
It enables the sorting and processing of food samples ranging from 10 to 50g, avoiding cross-contamination, improving sample processing efficiency and DNA extraction effectiveness, and ensuring the accuracy and safety of test results.
Smart Images
Figure CN224030981U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to food and meat product's gene detection, and more particularly, relate to a kind of 10-50g food sample gene detection sample pretreatment device. BACKGROUND
[0002] In the gene detection process of food and meat products, sample pretreatment is a crucial step. However, the existing sample pretreatment device has many defects and deficiencies in processing such samples. Traditional grinders have obvious limitations in sample capacity. For example, the common Tissuelyser-24L grinder uses 2mL centrifuge tubes, which can only process ≤5g samples. This cannot meet the needs of meat samples commonly used in food detection, such as 50g muscle tissue. This capacity limitation not only reduces the efficiency of sample processing, but also may lead to insufficient fragmentation of the sample, thereby affecting the extraction of DNA and the accuracy of subsequent detection. For example, the existing equipment lacks differentiation of different sample types in program settings. Hard samples such as bones are prone to high temperatures during grinding, and high-temperature environments pose a threat to the integrity of DNA, thereby affecting the reliability of gene detection. In addition, for soft tissue samples, the existing equipment cannot dynamically adjust the grinding parameters according to the sample resistance, resulting in excessive grinding of soft tissue and a 15% decrease in DNA yield. This "one-size-fits-all" grinding method cannot meet the individual processing needs of different types of samples, seriously affecting the accuracy and consistency of the detection results. Moreover, cross-contamination is another important challenge faced by existing equipment. When metal blades are reused, even after high-temperature sterilization, the residual organic contamination rate is still as high as 0.7% (ISO 22174:2019 standard requires <0.1%). This cross-contamination not only affects the accuracy of the detection results, but also may lead to false positives in food safety testing, posing potential risks. SUMMARY
[0003] To address the above problems, the utility model provides a sample pretreatment device for 10-50g food sample gene detection. The sample pretreatment device for 10-50g food sample gene detection aims to improve at least one problem mentioned in the background art.
[0004] The application discloses a sample pretreatment device for gene detection of 10-50g food sample, which comprises a lower platform, wherein a first food sample pretreatment unit, a second food sample pretreatment unit, a third food sample pretreatment unit, a fourth food sample pretreatment unit, a fifth food sample pretreatment unit and a sixth food sample pretreatment unit are sequentially arranged on the lower platform.
[0005] Optionally, the sample pretreatment device further comprises an upper platform, wherein the lower platform and the upper platform are connected through four supporting columns, and the upper platform is provided with a power system for driving the first food sample pretreatment unit, the second food sample pretreatment unit, the third food sample pretreatment unit, the fourth food sample pretreatment unit, the fifth food sample pretreatment unit and the sixth food sample pretreatment unit to perform pretreatment.
[0006] Optionally, each food sample pretreatment unit further comprises a lifting platform, wherein a mounting platform of the pretreatment container is mounted on the top of the lifting platform, and a mounting structure of the pretreatment container is formed in the mounting platform.
[0007] Optionally, the lifting platform is a scissor-type hydraulic lifting platform.
[0008] Optionally, the mounting structure of the pretreatment container comprises an outer groove, wherein a boss is arranged at the middle position of the outer groove, an inner groove is arranged at the middle position of the boss, the depth of the outer groove is higher than the depth of the sample area of the pretreatment container, the depth of the inner groove is lower than the depth of the outer groove, and the shape of the inner groove is matched with the bottom of the pretreatment container.
[0009] Optionally, a guiding and stabilizing platform is further arranged on the mounting platform, wherein the guiding and stabilizing platform is mounted on the mounting platform through supporting legs, and a hole allowing the pretreatment container to pass through is formed in the guiding and stabilizing platform.
[0010] Optionally, a labeling area is arranged on the lower platform corresponding to each food sample pretreatment unit, or the pretreatment container is a container with a cover, and a hole allowing the rotating shaft to pass through is formed in the cover.
[0011] Optionally, the pretreatment container is a 50ml centrifugal tube.
[0012] Optionally, the rotating cutting blade set comprises an upper blade, a middle blade and a lower blade, the upper blade and the middle blade are oppositely arranged on the shaft of the blade set, the distance between the end of the upper blade and the wall of the 50ml centrifuge tube is smaller than the distance between the end of the middle blade and the wall of the 50ml centrifuge tube, and the lower blade is in the shape of a reversed gun head.
[0013] Optionally, the power system comprises a motor and a plurality of electromagnetic clutches corresponding to the number of rotating shafts, the motor is connected with each electromagnetic clutch, each electromagnetic clutch is connected with a rotating shaft, and the number of teeth of the driven wheels mounted on the rotating shafts is not equal.
[0014] Compared with the prior art, the utility model has the beneficial effects that:
[0015] The utility model can process 10-50g food sample pretreatment by categories, and there is no cross contamination problem. BRIEF DESCRIPTION OF DRAWINGS
[0016] In order to more clearly illustrate the technical scheme in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description are some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor under the premise of the drawings.
[0017] Figure 1 It is the whole structure schematic diagram of the utility model;
[0018] Figure 2 It is the schematic diagram of the installation of the pretreatment container and the rotating cutting unit of the utility model;
[0019] Figure 3 It is the structure schematic diagram of the lifting platform, the installation platform and the guiding stable platform of the utility model;
[0020] Figure 4 It is the top view schematic diagram of the installation structure of the pretreatment container of the utility model;
[0021] Figure 5 It is the schematic diagram of the principle of the electric power transmission of the utility model.
[0022] Explanation of reference signs: 1, lower platform, 2, upper platform, 3, support column, 4, first food sample sample pretreatment unit, 5, second food sample sample pretreatment unit, 6, third food sample sample pretreatment unit, 7, fourth food sample sample pretreatment unit, 8, fifth food sample sample pretreatment unit, 9, pretreatment container, 10, rotary cutting unit, 11, rotary shaft, 12, rotary cutting blade set, 121, upper blade, 122, middle blade, 123, lower blade, 13, sixth food sample sample pretreatment unit, 14, labeling area, 15, lifting platform, 16, mounting platform, 17, pretreatment container mounting structure, 171, outer groove, 172, boss, 173, inner groove, 18, guide stable platform, 19, driving wheel, 20, driven wheel, 21, electromagnetic clutch. DETAILED DESCRIPTION
[0023] In the description of the present application, it should be explained that, unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connection" should be understood in a broad sense, for example, it can be fixed connection, or indirect connection through intermediate medium, or internal communication of two elements or interaction relationship of two elements. For ordinary skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.
[0024] In the description of the present application, it should be understood that the terms "upper", "lower", "front", "rear", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, therefore it cannot be understood as a limitation on the present application. In the description of the present application, the meaning of "a plurality of" is two or more, unless otherwise specified.
[0025] The terms "first", "second", "third", "fourth" and the like (if any) in the description of the present application and claims and the above drawings are used to distinguish similar objects, and do not necessarily indicate a specific order or sequence. It should be understood that the data thus used can be interchanged under appropriate circumstances, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion, for example, a process, method, system, product or device including a series of steps or units does not necessarily limit to those steps or units clearly listed, but can include other steps or units not clearly listed or inherent to these processes, methods, products or devices.
[0026] So that the purposes, technical solutions and advantages of the embodiments of the present application are more apparent, the technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of the present application.
[0027] The technical solutions of the present application will be described in detail below with specific embodiments. The following specific embodiments can be combined with each other, and the same or similar concepts or processes can not be described in some embodiments.
[0028] Please refer to Figures 1-5 , Figure 1 is a schematic diagram of the overall structure of the present application, Figure 2 is a schematic diagram of the present application after the front treatment container and the rotary cutting unit are installed, Figure 3 is a schematic diagram of the structure of the lifting platform, the installation platform and the guide stable platform of the present application, Figure 4 is a schematic diagram of the top view of the installation structure of the front treatment container of the present application, Figure 5 is a schematic diagram of the principle of electric power transmission of the present application.
[0029] A sample pretreatment device for 10-50g food sample gene detection, comprising a lower platform 1 and an upper platform 2, the lower platform 1 and the upper platform 2 are connected by four supporting columns 3, a first food sample sample pretreatment unit 4, a second food sample sample pretreatment unit 5, a third food sample sample pretreatment unit 6, a fourth food sample sample pretreatment unit 7, a fifth food sample sample pretreatment unit 8 and a sixth food sample sample pretreatment unit 13 are arranged in sequence on the lower platform 1, and a power system (not shown in the figure) for driving the first food sample sample pretreatment unit 4, the second food sample sample pretreatment unit 5, the third food sample sample pretreatment unit 6, the fourth food sample sample pretreatment unit 7, the fifth food sample sample pretreatment unit 8 and the sixth food sample sample pretreatment unit 13 for pretreatment is installed on the upper platform 2. Figure 1
[0030] Each of the first food sample sample pre-treatment unit 4, the second food sample sample pre-treatment unit 5, the third food sample sample pre-treatment unit 6, the fourth food sample sample pre-treatment unit 7, the fifth food sample sample pre-treatment unit 8 and the sixth food sample sample pre-treatment unit 13 comprises a pre-treatment container 9 and a rotary cutting unit 10, the pre-treatment container 9 has a volume of 50ml, the rotary cutting unit 10 comprises a rotary shaft 11 and a rotary cutting blade set 12, the rotary cutting blade set 12 is connected to one end of the rotary shaft 11, the rotary cutting blade set 12 is located in a sample area in the pre-treatment container 9, and the other end of the rotary shaft 11 is located above the pre-treatment container 9.
[0031] By arranging the first food sample sample pre-treatment unit 4, the second food sample sample pre-treatment unit 5, the third food sample sample pre-treatment unit 6, the fourth food sample sample pre-treatment unit 7, the fifth food sample sample pre-treatment unit 8 and the sixth food sample sample pre-treatment unit 13, the food sample sample pre-treatment units are independent of each other, and each food sample sample pre-treatment unit is specially arranged to process different food, for example, some food sample sample pre-treatment units are arranged to process meat samples, and some food sample sample pre-treatment units are arranged to process soft tissue samples, the pre-treatment container 9 has a volume of 50ml, the single processing capacity is increased by 10 times at most, and 10-50g of meat samples can be processed.
[0032] In one or more specific embodiments of the utility model, in order to facilitate the pre-treatment container 9 to be taken out, each food sample sample pre-treatment unit further comprises a lifting platform 15, the lifting platform 15 is provided with a mounting platform 16 of the pre-treatment container 9 on the top, and the mounting platform 16 is provided with a pre-treatment container mounting structure 17. By the lifting platform 15, when processing is needed, after the sample and the like are mounted, the lifting platform 15 is lifted to stop lifting after the rotary cutting blade set 12 is located in the sample area in the pre-treatment container 9, rotary cutting is started, after processing is completed, the lifting platform 15 is lowered, and the pre-treatment container 9 is taken out, and the whole process is very convenient.
[0033] In one or more specific embodiments of the utility model, in order to remove the heat generated during the pre-treatment, the pre-treatment container mounting structure 17 includes an outer groove 171, the middle position of the outer groove 171 is provided with a boss 172, the middle position of the boss 172 is provided with an inner groove 173, the depth of the outer groove 171 is higher than the sample area depth of the pre-treatment container 9, the depth of the inner groove 173 is lower than the depth of the outer groove 171, and the shape of the inner groove 173 is matched with the bottom of the pre-treatment container 9. Through the setting of the outer groove 171 and the inner groove 173, the heat generated during the operation can be removed by the heat transfer mode by passing the cold fluid into the outer groove 171. In order to achieve better heat dissipation effect, the wall of the boss 172 between the inner groove 173 and the outer groove 171 should not be too thick, and the material of the boss 172 is also selected to be a material with good thermal conductivity. In order to prevent the cold quality passing into the outer groove 171 from being exposed, the exposed part of the outer groove 171 can be covered with a cover plate.
[0034] In one or more specific embodiments of the utility model, in order to install more stably and prevent the pre-treatment container from deviating, a guide stable platform 18 is further arranged on the mounting platform 16, the guide stable platform 18 is mounted on the mounting platform 16 through a supporting leg, and a hole allowing the pre-treatment container 9 to pass through is formed in the guide stable platform 18.
[0035] In one or more specific embodiments of the utility model, the lifting platform 15 is a scissor type hydraulic lifting platform.
[0036] In one or more specific embodiments of the utility model, in order to facilitate installation, the rotating shaft 11 and the rotating cutting blade group 12 can be connected integrally in a threaded connection mode. In the utility model, in order to avoid affecting the detection quality, the rotating cutting blade group 12 is a disposable consumable.
[0037] In one or more specific embodiments of the utility model, in order to facilitate identification, the lower platform 1 is provided with a labeling area 14 corresponding to each food sample sample pre-treatment unit, and the processing category and processing condition of the corresponding food sample sample pre-treatment unit are marked and explained in the labeling area 14.
[0038] In one or more specific embodiments of the utility model, in order to prevent the sample from splashing out during the operation, the pre-treatment container 9 is a container with a cover, and the cover is provided with a hole allowing the rotating shaft 11 to enter and exit.
[0039] In one or more specific embodiments of the present application, the pre-treatment container 9 is a 50ml centrifuge tube. The 50ml centrifuge tube can be directly purchased from the market, and the pre-treatment container 9 can be conveniently replaced as a consumable. The same tube can be used multiple times and cause pollution. At the same time, the bottom of the 50ml centrifuge tube is conical, which is also convenient to place. Before work, only need to open a hole on the purchased 50ml centrifuge tube, fill the sample, and install the rotary cutting unit 10, then the work can be started.
[0040] In one or more specific embodiments of the present application, in order to obtain better pre-treatment effect, according to the structure of the 50ml centrifuge tube, the rotary cutting blade set 12 includes an upper blade 121, a middle blade 122 and a lower blade 123, the upper blade 121 and the middle blade 122 are reversely arranged on the shaft of the blade set, the distance between the end of the upper blade 121 and the tube wall of the 50ml centrifuge tube is less than the distance between the end of the middle blade 122 and the tube wall of the 50ml centrifuge tube, and the lower blade 123 is in the shape of an inverted gun head.
[0041] Those skilled in the art should know that the power system can be an electric power system or a hydraulic drive system. How the power system is arranged and installed on the upper platform 2 is not specifically limited in the present application, and those skilled in the art can arrange and install it as needed. The upper platform 2 has holes allowing each rotary shaft 11 to pass through, and the upper end of each rotary shaft 11 passes through the upper platform 2 and is fixed by bearing.
[0042] In order to reduce the cost, in another specific embodiment of the present application, all rotary shafts 11 are connected to the same motor, and all rotary shafts 11 are driven to rotate by the same motor. In order to realize that some of the rotary shafts 11 connected to the same motor rotate when the motor is turned on, and some do not work when the motor is turned on, as shown in Figure 5 An electromagnetic clutch 21 is connected between the driving wheel 19 installed on the motor output shaft and the driven wheel 20 installed on the rotary shaft 11, and the driven wheel 20 is controlled to be in working and non-working state by the electromagnetic clutch 21. In order to adapt to different food processing, the rotational speed of each rotary shaft 11 is adapted, and the number of teeth of each driven wheel 20 is not equal.
[0043] Finally, it should be noted that: the above embodiments are used to illustrate the technical solutions of the present application, but not limited to them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.
Claims
1. A sample pretreatment device for 10-50 g food sample genetic detection, characterized in that, The sample pretreatment device comprises a lower platform (1), a first food sample sample pretreatment unit (4), a second food sample sample pretreatment unit (5), a third food sample sample pretreatment unit (6), a fourth food sample sample pretreatment unit (7), a fifth food sample sample pretreatment unit (8) and a sixth food sample sample pretreatment unit (13) are arranged in sequence on the lower platform (1), each food sample sample pretreatment unit comprises a pretreatment container (9) and a rotary cutting unit (10), the volume of the pretreatment container (9) is 50ml, the rotary cutting unit (10) comprises a rotary shaft (11) and a rotary cutting blade set (12), the rotary cutting blade set (12) is connected to one end of the rotary shaft (11), the rotary cutting blade set (12) is located in the sample area in the pretreatment container (9), and the other end of the rotary shaft (11) is located above the pretreatment container (9).
2. The sample pretreatment device for 10-50 g food sample gene detection according to claim 1, characterized in that, The sample pretreatment device further comprises an upper platform (2), the lower platform (1) and the upper platform (2) are connected through four support columns (3), and the upper platform (2) is provided with a power system for driving the first food sample sample pretreatment unit (4), the second food sample sample pretreatment unit (5), the third food sample sample pretreatment unit (6), the fourth food sample sample pretreatment unit (7), the fifth food sample sample pretreatment unit (8) and the sixth food sample sample pretreatment unit (13) to perform pretreatment.
3. The sample pretreatment device for 10-50 g food sample gene detection according to claim 2, characterized in that, Each food sample sample pretreatment unit further comprises a lifting platform (15), a mounting platform (16) of the pretreatment container (9) is mounted on the top of the lifting platform (15), and a pretreatment container mounting structure (17) is formed in the mounting platform (16).
4. The sample pretreatment device for 10-50 g food sample genetic detection according to claim 3, characterized in that, The lifting platform (15) is a scissor-type hydraulic lifting platform.
5. The sample pretreatment device for 10-50 g food sample genetic detection according to claim 3, characterized in that, The pretreatment container mounting structure (17) comprises an outer groove (171), a boss (172) is arranged at the middle position of the outer groove (171), an inner groove (173) is arranged at the middle position of the boss (172), the depth of the outer groove (171) is higher than the sample area depth of the pretreatment container (9), the depth of the inner groove (173) is lower than the depth of the outer groove (171), and the shape of the inner groove (173) is matched with the bottom of the pretreatment container (9).
6. The sample pretreatment device for 10-50 g food sample gene detection according to claim 3, characterized in that, The mounting platform (16) is further provided with a guide stabilizing platform (18), the guide stabilizing platform (18) is mounted on the mounting platform (16) through a support leg, and a hole allowing the pretreatment container (9) to pass through is formed in the guide stabilizing platform (18).
7. The sample pretreatment device for 10-50 g food sample genetic detection according to claim 1, characterized in that, The lower platform (1) is provided with a labeling area (14) corresponding to each food sample sample pretreatment unit, or the pretreatment container (9) is a container with a cover, and a hole allowing the rotary shaft (11) to pass in and out is formed in the cover.
8. The sample pretreatment device for 10-50 g food sample genetic detection according to claim 1, characterized in that, The pretreatment container (9) is a 50ml centrifuge tube.
9. The sample pretreatment device for 10-50 g food sample gene detection according to claim 8, characterized in that, The rotating cutting blade set (12) comprises an upper blade (121), a middle blade (122) and a lower blade (123), the upper blade (121) and the middle blade (122) are oppositely arranged on the shaft of the blade set, the distance between the end of the upper blade (121) and the wall of the 50ml centrifugal tube is less than the distance between the end of the middle blade (122) and the wall of the 50ml centrifugal tube, and the lower blade (123) is in the shape of inverted gun head.
10. The sample pretreatment device for 10-50 g food sample genetic detection according to claim 2, characterized in that, The power system comprises a motor and electromagnetic clutches (21) corresponding in number to the rotating shafts (11), the motor is connected with the electromagnetic clutches (21) respectively, each electromagnetic clutch (21) is connected with a rotating shaft (11), and the number of teeth of the driven wheels (20) mounted on the rotating shafts (11) is not equal.