Disposable exosome separating device

Abstract

The utility model discloses a disposable exosome separating device which comprises a separating bin and a cover plate, the cover plate is installed at the top of the separating bin, a separating mechanism is arranged in the separating bin and comprises separating pipes and a rotating bin, the rotating bin is arranged on the bottom wall of the separating bin, the separating pipes are evenly distributed at the top of the rotating bin, and the rotating bin is arranged on the bottom wall of the separating bin. According to the exosome extraction device, the disposable test tube is used for placing exosomes, and the test tube is subjected to differential centrifugal treatment through the separation mechanism, so that the problems that in the background technology, the exosomes are mainly extracted by adopting a reusable tube body, the tube body needs to be treated independently before and after use, and residues are avoided are solved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to exosome separation technical field, and particularly relates to disposable exosome separation device. BACKGROUND

[0002] Exosome is a kind of small vesicle released by cell, and contains lipid bilayer encapsulated bioactive molecules, such as miRNA, protein and extracellular matrix component.Exosome has extensive biological function, and has huge application potential in the fields of regenerative medicine, drug delivery and disease treatment.

[0003] At present, the method for extracting exosome mainly has differential centrifugation, ultrafiltration, precipitation and affinity chromatography, but the existing extraction is mainly to place exosome by reusable tube body, and the tube body needs to be handled separately before and after use to avoid residue, therefore, the disposable exosome separation device is proposed. UTILITY MODEL CONTENT

[0004] The utility model aims at overcoming the problem that the existing technology mainly uses reusable tube body to place exosome, and the tube body needs to be handled separately before and after use to avoid residue, and provides a disposable exosome separation device using disposable test tube to place exosome, and the test tube is subjected to differential centrifugation treatment by separation mechanism.

[0005] In order to realize the above-mentioned purpose, the utility model provides disposable exosome separation device, including separation bin and apron, the apron installation is located separation bin top, the separation bin is equipped with separation mechanism, the separation mechanism includes separation tube and rotating bin, the rotating bin is located separation bin bottom wall, the separation tube evenly distributes and is equipped with the rotating bin top.

[0006] Preferably, the separation tube includes tube body, cushion block, drive screw, drive motor and push tube, the tube body is equipped on the rotating bin, the disposable test tube is placed in the tube body, the drive motor is equipped on the bottom wall of the tube body, the drive screw is fixedly connected to the drive motor, the push tube is sleeved on the drive screw, the push tube is equipped with the internal thread structure that the drive screw outer side surface is in line with, the cushion block is equipped on the push tube top, and the arc recess structure is arranged on the top of the cushion block.

[0007] Preferably, the inner wall of the tube body is symmetrically provided with a slide rail, a sliding block is movably sleeved on the slide rail, and the sliding block is fixedly connected to the lower end of the side surface of the push tube.

[0008] Preferably, a partition plate is arranged in the middle of the inner wall of the tube body, and the partition plate is sleeved on the push tube and arranged on the slide rail.

[0009] Preferably, the cushion block is evenly provided with a pulley on both sides, and the pulley is movably arranged on the side surface of the inner wall of the tube body.

[0010] Preferably, the pipe body is provided with an inner lining block.

[0011] Preferably, the top of the cover plate is provided with a handle, and the lower cover plate is provided with an extension ring, and the outer diameter of the extension ring is the same as the inner diameter of the separation bin.

[0012] The beneficial effects of the present application are:

[0013] The present application uses a disposable test tube to place the exosome, and the test tube is subjected to differential centrifugal treatment through the separation mechanism, solving the problem of repeatedly using the pipe body to place the exosome in the background art, and the pipe body needs to be treated separately before and after use to avoid residue. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is a whole structure schematic view of the disposable exosome separation device of the present application.

[0015] Figure 2 It is an internal structure diagram of the disposable exosome separation device of the present application.

[0016] Figure 3 It is a structure diagram of the separation mechanism in the disposable exosome separation device of the present application.

[0017] Figure 4 It is an internal structure diagram of the separation tube in the disposable exosome separation device of the present application.

[0018] Figure 5 It is Figure 4 A local enlarged view of A in the figure.

[0019] In the figure: 1, separation bin; 2, cover plate; 3, separation mechanism; 4, separation tube; 5, rotating bin; 6, pipe body; 7, cushion block; 8, driving screw; 9, driving motor; 10, push tube; 11, slide rail; 12, sliding block; 13, partition; 14, pulley; 15, inner lining block; 16, handle; 17, extension ring. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present application are described below, and obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Figures 1-5 Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0021] In the description of the utility model, it is understood that the terms "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the utility model, and do not indicate or imply that the device or element referred to must have a particular orientation, a particular orientation structure and operation, so it cannot be understood as a limitation on the utility model.

[0022] The utility model provides a disposable exosome separation device, including separation bin 1 and cover plate 2, cover plate 2 installs in separation bin 1 top, cover plate 2 top is equipped with handle 16, cover plate 2 below is equipped with extension ring 17, the outer diameter of extension ring 17 is same with the inside diameter size of separation bin 1, separation bin 1 is equipped with separation mechanism 3 in, separation mechanism 3 includes separation pipe 4 and rotary bin 5, rotary bin 5 is equipped on separation bin 1 bottom wall, separation pipe 4 evenly distributes and is equipped in rotary bin 5 top.

[0023] In an embodiment, specifically, separation pipe 4 includes pipe body 6, cushion block 7, drive screw 8, drive motor 9 and push tube 10, pipe body 6 is equipped on rotary bin 5, disposable test tube is placed in pipe body 6, drive motor 9 is equipped on pipe body 6 bottom wall, drive screw 8 is fixedly connected to drive motor 9, push tube 10 is sleeved on drive screw 8, push tube 10 is equipped with the internal thread structure that drive screw 8 outer side surface is in line with, cushion block 7 is equipped in push tube 10 top, cushion block 7 top is equipped with arc recess structure, the inner wall of pipe body 6 is equipped with sliding rail 11 symmetrically, sliding block 12 is movably sleeved on sliding rail 11, sliding block 12 is fixedly connected to push tube 10 side lower end, the inner wall of pipe body 6 is equipped with baffle 13 in the middle, baffle 13 is sleeved on push tube 10 and is equipped on sliding rail 11, cushion block 7 both sides are evenly distributed and are equipped with pulley 14, pulley 14 moves and is equipped on the inner wall side of pipe body 6, pulley 14 improves the stability of cushion block 7 lifting, pipe body 6 is equipped with inner lining 15, drive motor 9 drives drive screw 8 to rotate, drive screw 8 drives push tube 10 to lift, push tube 10 promotes cushion block 7 and test tube to rise, test tube is ejected from pipe body 6, and it is convenient for staff to take test tube away.

[0024] The working process of the disposable exosome separation device in the embodiment is as follows: the test tube containing exosomes is placed in the pipe body 6, then the lower end of the test tube falls on the cushion block 7, then the cover plate 2 is placed on the separation bin 1, the extension ring 17 is inserted into the separation bin 1, the rotary bin 5 drives the separation pipe 4 and the test tube to rotate, and the exosome separation is performed, after the separation is completed, the cover plate 2 is removed through the handle 16, then the drive motor 9 is started, the drive motor 9 drives the drive screw 8 to rotate, the drive screw 8 drives the push tube 10 to lift, the push tube 10 promotes the cushion block 7 and the test tube to rise, the test tube is ejected from the pipe body 6, and the staff takes away the test tube.

[0025] The above is only an example and description of the structure of the utility model, and those skilled in the art can make various modifications or supplements to the described specific embodiments or replace them with similar ways, as long as they do not deviate from the structure of the utility model or exceed the scope defined by the present claims, which shall belong to the protection scope of the utility model.

Claims

1. A disposable exosome separation device, characterized in that, It includes a separation chamber (1) and a cover plate (2). The cover plate (2) is installed on the top of the separation chamber (1). The separation chamber (1) is provided with a separation mechanism (3). The separation mechanism (3) includes a separation tube (4) and a rotating chamber (5). The rotating chamber (5) is located on the bottom wall of the separation chamber (1). The separation tube (4) is evenly distributed on the top of the rotating chamber (5). The separation tube (4) includes a tube body (6), a pad (7), a drive screw (8), a drive motor (9), and a push tube (10). The tube body (6) is located on the rotating chamber (5), and a disposable test tube is placed inside the tube body (6). The drive motor (9) is located on the bottom wall of the tube body (6). The drive screw (8) is fixedly connected to the drive motor (9). The push tube (10) is sleeved on the drive screw (8). The push tube (10) has an internal thread structure that matches the outer side of the drive screw (8). The pad (7) is located on the top of the push tube (10), and the top of the pad (7) has an arc-shaped recessed structure.

2. The disposable exosome separation device according to claim 1, characterized in that, The inner wall of the tube (6) is symmetrically provided with slide rails (11), and a slider (12) is movably sleeved on the slide rails (11). The slider (12) is fixedly connected to the lower side of the push tube (10).

3. The disposable exosome separation device according to claim 2, characterized in that, The inner wall of the tube (6) is provided with a partition (13) in the middle, and the partition (13) is sleeved on the push tube (10) and provided on the slide rail (11).

4. The disposable exosome separation device according to claim 1, characterized in that, The pad (7) is provided with pulleys (14) evenly distributed on both sides, and the pulleys (14) are movably located on the inner wall side of the tube (6).

5. The disposable exosome separation device according to claim 1, characterized in that, An inner lining block (15) is provided between the tubes (6).

6. The disposable exosome separation device according to claim 1, characterized in that, The cover plate (2) is provided with a handle (16) at the top and an extension ring (17) is provided under the cover plate (2). The outer diameter of the extension ring (17) is the same as the inner diameter of the separation chamber (1).

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