Movable cell culture fluid infusion device

Abstract

The utility model discloses a movable cell culture fluid infusion device which comprises a moving component and a clamping component, an objective table for placing the clamping component is mounted at the top of the moving component, and a hanging component is mounted on the moving component; the clamping assembly comprises a bearing partition plate and an opening and closing device, a sliding assembly which is connected with the opening and closing device and allows the opening and closing device to move is installed at the top of the bearing partition plate, and a plurality of air holes are formed in the bearing partition plate. The bearing partition plate provides a bearing effect for the culture bag, meanwhile, good gas exchange in the culture box is guaranteed, the balance of the concentration of carbon dioxide and oxygen is helped to be maintained, the size of the bearing partition plate can be determined according to the size of the culture box, the adaptability of the bearing partition plate and the culture box is guaranteed, and the bearing partition plate is convenient to put in and take out.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture device field, concretely relates to a movable cell culture liquid supplementing device. BACKGROUND

[0002] In the cell culture process, the number and density of cells will increase with the increase of culture time, and the culture volume will also increase. The traditional liquid supplementing method is usually carried out when the number of cells is small and the culture volume is small, and after the number of cells is expanded, the container such as a culture bottle or a culture bag needs to be replaced due to the change in volume; replacing the culture container not only increases the risk of cell contamination, but also may affect the activity of cells due to too long liquid supplementing time, especially for cells in suspension culture, it is best to provide mixing conditions during liquid supplementing, otherwise the state of cells may be affected, therefore, there is an urgent need for a liquid supplementing device that can flexibly adjust the culture volume and provide mixing conditions during cell culture according to needs. SUMMARY

[0003] The utility model solves the problems of complex cell culture liquid supplementing operation, high contamination risk and inability of cells to mix during the liquid supplementing process in the prior art, and provides a movable cell culture liquid supplementing device, which can flexibly adjust the optimal bottom area of a cell culture bag and the height of a culture medium liquid surface through a clamping assembly, improve the gas exchange rate of the culture system, control the contact space between cells, and be beneficial to cell expansion culture; the replacement steps between different specifications of culture bags required by the conventional method can be avoided through a bearing partition, the cost is reduced, and the risk of cell contamination caused by replacement of culture bags is greatly reduced; the object table provides cell mixing conditions, which is beneficial to the growth and activity maintenance of cells, especially for cells in suspension; the device is simple to operate, can be used alone according to different culture needs, or can be used together, is suitable for cell culture and liquid supplementing operation in the fields of biological medicine and biotechnology, has a simple structure, is convenient to use, and is used to solve the defects caused by the prior art.

[0004] To solve the above technical problems, the utility model provides the following technical scheme:

[0005] A movable cell culture liquid supplementing device, wherein a mobile assembly and a clamping assembly are included, the top of the mobile assembly is provided with an object table on which the clamping assembly is placed, and a hanging assembly is installed on the mobile assembly;

[0006] The clamping assembly comprises a bearing partition and an opening and closing device, the bearing partition is provided with a sliding assembly connected with the opening and closing device at the top, and a plurality of air holes are formed in the bearing partition, in use, the culture bag is placed on the bearing partition and fixed by the opening and closing device, and then the culture bag can be placed in a culture box, the bearing partition provides bearing for the culture bag and ensures good gas exchange in the culture box, thereby helping to maintain the balance of carbon dioxide and oxygen concentration.

[0007] The movable cell culture liquid supplementing device, wherein the moving assembly comprises a fixed frame and a fixed plate installed on the top of the fixed frame, the object table is installed on the top of the fixed plate, a plurality of pulleys are installed on the bottom of the fixed frame, and the fixed plate can be placed with liquid supplementing related equipment such as a peristaltic pump, a connecting pipe machine, a heat sealing instrument and a counter, and the fixed frame can be moved to a position required for liquid supplementing operation according to needs through the pulleys.

[0008] The movable cell culture liquid supplementing device, wherein a universal support connected to the top of the fixed plate is installed on the bottom of the object table, the object table is used for placing and fixing the bearing partition or the culture bag, the direction of the object table can be changed for angle adjustment through the universal support, the size of the object table can be freely customized, and small instrument equipment can be carried according to needs.

[0009] The movable cell culture liquid supplementing device, wherein the suspension assembly comprises a fixed rod installed on one side of the fixed frame and a hook support adjustably installed on the fixed rod, a plurality of hooks are installed on the hook support, the hook support is rotatable, and the entire fixed frame can be moved through the pulleys to facilitate movement to a specified area.

[0010] The movable cell culture liquid supplementing device, wherein the sliding assembly comprises two fixed supports symmetrically arranged, and a sliding rail is installed on the top of each fixed support, and two ends of the opening and closing device are respectively installed on the two sliding rails.

[0011] The movable cell culture liquid supplementing device, wherein a plurality of fixed clamps are installed on the top of the bearing partition.

[0012] The movable cell culture liquid supplementing device, wherein four fixed clamps are arranged on the inside of the fixed supports at four corners of the bearing partition.

[0013] The movable cell culture liquid supplementing device, wherein a scale is installed on one side of the bearing partition.

[0014] The mobile cell culture liquid supplementing device relates to the fields of biological medicine, biotechnology and medical instrument equipment, and comprises a moving assembly and a clamping assembly, the clamping assembly comprises a bearing partition plate, a support, a sliding rail, an opener, a fixed clamp and a scale ruler, can adjust the cavity size required by a culture bag in a cell culture process, realizes one-bag culture from small volume to large volume, reduces the cell pollution risk caused by replacement of a culture container, the moving assembly comprises a fixed frame, a carrier table, a universal support, a hook and a pulley, the universal support can swing and is suitable for liquid supplementing of suspended cells, the device is simple in operation, easy to control, high in expandability, can enhance the stability of culture and liquid supplementing operation, is favorable for in-vitro mass expansion culture of cells, can reduce the risk of cell pollution caused by liquid supplementing, guarantees the quality of cultured cells, and is suitable for cell culture liquid supplementing operation in the fields of biological medicine and biotechnology.

[0015] The technical scheme of the mobile cell culture liquid supplementing device provided by the utility model has the following technical effects:

[0016] The clamping assembly can flexibly adjust the optimal bottom area of the cell culture bag and the height of the culture medium liquid surface, improves the gas exchange rate of the culture system, controls the contact space between cells, and is favorable for cell expansion culture; the bearing partition plate can eliminate the replacement steps between different specifications of conventional culture bags, reduces the cost, and greatly reduces the cell pollution risk caused by replacement of the culture bag; the carrier table provides cell mixing conditions, is favorable for cell growth and activity maintenance, especially for suspended cells; the device is simple in operation, can be used alone or together according to different culture needs, is suitable for cell culture and liquid supplementing operation in the fields of biological medicine and biotechnology, and is simple in structure and convenient to use. BRIEF DESCRIPTION OF DRAWINGS

[0017] Fig. 1 FIG. 1 is a structural schematic view of a moving assembly in the mobile cell culture liquid supplementing device;

[0018] Fig. 2 FIG. 2 is a structural schematic view of a clamping assembly in the mobile cell culture liquid supplementing device.

[0019] In the drawings, the reference signs are as follows:

[0020] The moving assembly 100, the clamping assembly 200, the carrier table 101, the fixed frame 102, the fixed plate 103, the pulley 104, the universal support 105, the fixed rod 106, the hook support 107, the hook 108, the bearing partition plate 201, the opener 202, the air hole 203, the fixed support 204, the sliding rail 205, the fixed clamp 206 and the scale ruler 207. DETAILED DESCRIPTION

[0021] In order to make the technical means, creative features, purposes and effects of the utility model easy to understand, the technical solutions in the embodiments of the utility model are clearly and completely described below in combination with specific drawings. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments.

[0022] Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the utility model.

[0023] It should be understood that the structures, proportions, sizes and the like shown in the drawings attached to the specification are only used to cooperate with the disclosed content, to be understood and read by those skilled in the art, and do not define the limiting conditions for the implementation of the utility model, so they do not have technical substantive significance. Any modification of structure, change of proportion relationship or adjustment of size, without affecting the effects and purposes that can be achieved by the utility model, should still fall within the scope of the disclosed technical content of the utility model.

[0024] At the same time, the terms such as "up", "down", "left", "right", "middle" and "one" cited in the specification are only for the convenience of clear description, and are not used to limit the scope of implementation of the utility model. The change or adjustment of the relative relationship is also considered as the scope of implementation of the utility model without substantial change of technical content.

[0025] A preferred embodiment of the utility model provides a movable cell culture liquid supplementing device, which can flexibly adjust the optimal bottom area of the cell culture bag and the height of the culture medium liquid surface through the clamping assembly, improve the gas exchange rate of the culture system, control the contact space between cells, and is beneficial to cell expansion culture. The replacement steps between different specifications of conventional culture bags can be avoided through the bearing partition plate, the cost is reduced, and the risk of cell pollution caused by replacement of the culture bag is also greatly reduced. The object table provides cell mixing conditions, which is beneficial to the growth and activity maintenance of cells, especially for suspended cells. The device is simple to operate, can be used alone according to different culture needs, can also be used together, is suitable for cell culture and liquid supplementing operation in the field of biological medicine and biotechnology, has simple structure and is convenient to use.

[0026] As shown in the drawings, Figs. 1-2 A movable cell culture liquid supplementing device, which comprises a moving assembly 100 and a clamping assembly 200. The moving assembly 100 is provided with a loading table 101 on the top for placing the clamping assembly 200. A hanging assembly is installed on the moving assembly 100, which can be used alone.

[0027] The clamping assembly 200 comprises a bearing partition plate 201 and an opening and closing device 202, the top of the bearing partition plate 201 is provided with a sliding assembly connected with the opening and closing device 202 for moving the opening and closing device 202, a plurality of air holes 203 are formed in the bearing partition plate 201, in use, the culture bag is placed on the bearing partition plate 201 and fixed by the opening and closing device 202, and then the culture bag can be placed in the incubator, the bearing partition plate 201 provides bearing for the culture bag and ensures good gas exchange in the incubator, helps to maintain the balance of carbon dioxide and oxygen concentration, the size of the bearing partition plate 201 can be determined according to the size of the incubator to ensure the adaptability with the incubator, and the bearing partition plate 201 and the cell culture bag can be placed in the incubator for normal culture, and when liquid supplementing is needed, the bearing partition plate 201 is taken out together with the cell culture bag for liquid supplementing operation.

[0028] The movable cell culture liquid supplementing device has the following advantages.

[0029] The movable cell culture liquid supplementing device has the following advantages.

[0030] The movable cell culture liquid supplementing device has the following advantages.

[0031] The movable cell culture liquid supplementing device has the following advantages. The movable cell culture liquid supplementing device has the following advantages.

[0032] The mobile cell culture liquid supplementing device, wherein a plurality of fixing clamps 206 are installed on the top of the bearing partition plate 201, the plurality of fixing clamps 206 are arranged at four corners of the bearing partition plate 201 and inside the fixing support 204, the fixing clamps 206 can fix the cell culture bag, facilitate the liquid supplementing operation, and can avoid directly taking the culture bag to reduce the risk of damaging the culture bag.

[0033] The mobile cell culture liquid supplementing device, wherein a scale ruler 207 is installed on one side of the bearing partition plate 201, the scale ruler 207 is used for positioning, can more accurately control the position of the opening and closing device 202, and makes the cell culture volume optimal.

[0034] The use steps of the mobile cell culture liquid supplementing device are as follows:

[0035] The cell culture bag is placed on the bearing partition plate 201, the size of the bottom area of the culture bag and the height of the liquid level of the culture solution are calculated according to the required culture volume, the opening and closing device 202 is moved to the appropriate position, and then the unused part of the culture bag is clamped, the process can be referred to the scale ruler 207 on the partition plate, the cell culture bag is placed in the incubator together with the bearing partition plate 201 for regular cell culture;

[0036] If the cell culture volume is small at the beginning and the liquid supplementing is required to a large volume in the subsequent culture process, the opening and closing device 202 is opened, the bottom area of the culture bag and the height of the liquid level of the culture solution are calculated, the opening and closing device 202 is moved to the appropriate position to clamp the unused part of the culture bag, if the size of the initially selected culture bag is suitable for the subsequent large volume cell culture, the opening and closing device 202 can be completely opened during the subsequent liquid supplementing;

[0037] The bearing partition plate 201 with the cell culture bag is taken out of the incubator, placed on the object table 101 and fixed, the required supplementing volume, the corresponding bottom area of the culture bag and the height of the liquid level are calculated, the opening and closing device 202 is moved to the appropriate position, the supplementing bag is hung on the hook 108, and the cell culture bag is connected by using a sterile pipe connecting machine and the like, the direction of the universal support 105 is adjusted to facilitate the operation, and the universal support 105 is manually shaken to swing left and right (the universal support 105 can be shaken or not shaken during the supplementing, and can be operated according to different cell supplementing requirements); then the peristaltic pump and the like are used for regular supplementing operation, after the supplementing operation is completed, the connection between the supplementing bag and the cell culture bag is disconnected by using a heat sealing instrument and the like, the cell culture bag is taken off from the object table 101 together with the bearing partition plate 201, and then placed in the incubator for continuous culture;

[0038] The mobile cell culture liquid supplementing device can be used as described above, and can also be used in other cell culture links, such as cell harvesting, cell liquid changing, cell transfer and the like.

[0039] In summary, the mobile cell culture liquid supplementing device can flexibly adjust the optimal bottom area of the cell culture bag and the height of the culture medium liquid surface through the clamping assembly, improve the gas exchange rate of the culture system, control the contact space between cells, and be conducive to cell expansion culture; the replacement steps between different specifications of conventional culture bags can be avoided through the bearing partition plate, the cost is reduced, and the cell pollution risk caused by the replacement of the culture bag is greatly reduced; the object table provides cell mixing conditions, is conducive to the growth and activity maintenance of cells, especially for suspended cells; the device is simple to operate, can be used alone according to different culture needs, can also be used together, is suitable for cell culture and liquid supplementing operation in the field of biological medicine and biotechnology, and has the advantages of simple structure and convenience in use.

[0040] The specific embodiments of the utility model are described above. It should be understood that the utility model is not limited to the above specific embodiments, and the devices and structures not fully described should be understood as being implemented in the ordinary way in the art; those skilled in the art can make various modifications or changes within the scope of the claims, make several simple deductions, modifications or replacements, which do not affect the essential content of the utility model.

Claims

1. A portable cell culture rehydration device, characterized in that, It includes a moving component and a clamping component. The moving component is equipped with a platform on top for placing the clamping component, and a suspension component is mounted on the moving component. The clamping assembly includes a support partition and an opening / closing device. A sliding component is installed on the top of the support partition to connect with the opening / closing device and allow the opening / closing device to move. The support partition has multiple ventilation holes.

2. The portable cell culture rehydration device according to claim 1, characterized in that, The moving component includes a fixed frame and a fixed plate mounted on top of the fixed frame, the platform is mounted on top of the fixed plate, and multiple casters are mounted on the bottom of the fixed frame.

3. The portable cell culture rehydration device according to claim 2, characterized in that, The bottom of the platform is equipped with a universal bracket connected to the top of the fixed plate.

4. A portable cell culture rehydration device according to claim 2, characterized in that, The suspension assembly includes a fixed rod mounted on one side of the fixed frame and a hook bracket adjustablely mounted on the fixed rod, wherein multiple hooks are mounted on the hook bracket.

5. A portable cell culture rehydration device according to claim 1, characterized in that, The sliding assembly includes two symmetrically arranged fixed supports, each of which has a slide rail mounted on its top, and the two ends of the opener are respectively mounted on the two slide rails.

6. A portable cell culture rehydration device according to claim 5, characterized in that, The top of the load-bearing partition is equipped with multiple fixing clips.

7. A portable cell culture rehydration device according to claim 6, characterized in that, Four fixing clips are provided, which are respectively located at the four corners of the bearing partition and inside the fixing bracket.

8. A portable cell culture rehydration device according to claim 1, characterized in that, A scale is installed on one side of the load-bearing partition.

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