Cell culture fluid collecting device capable of controlling flow velocity

Abstract

The utility model discloses a cell culture fluid collecting device capable of controlling flow velocity, which comprises a support and a fluid discharge rotating mechanism, the support comprises a base, two support rods fixedly connected to two sides of the base, and two lobster buckles fixedly connected to the tops of the two support rods respectively; the drainage rotating mechanism comprises a clamp, a rotating rod, a rotating gear, a silica gel gasket, a spring shifting piece and a rotating handle; the rotating rod penetrates through the lobster buckle and is fixedly connected with the clamp and the rotating gear, and the silica gel gasket is connected to the rotating rod in a sleeving mode and located between the lobster buckle and the rotating gear. The rotating handle is in threaded connection with the rotating rod; the spring shifting piece is fixed to the lobster buckle on the right side through a connecting block, and the thickness of the connecting block is the same as that of the silica gel gasket. The height of the cell culture bag is adjusted through the liquid discharge rotating mechanism, so that cell culture liquid is completely discharged due to gravity under the condition that the cell culture liquid is not extruded, and unnecessary waste is reduced while the cell activity is ensured; and compared with manual operation, the used time is shorter, and the efficiency is higher.

Description

TECHNICAL FIELD

[0001] The utility model relates to a culture device technical field especially, it relates to a cell culture solution collection device of controllable flow rate. BACKGROUND

[0002] In recent years, immune cell treatment technology develops rapidly at home and abroad, and shows great potential in dealing with various diseases. Immune cell therapy collects human peripheral blood or umbilical cord blood, separates immune cells in vitro, cultures, expands, and finally collects cells, and returns to the patient's body. When immune cells are cultured and expanded on a large scale, cell culture bags and a large amount of fresh culture medium are needed to provide nutrients required for cell growth; when collecting cells, cells and culture medium need to be discharged from the cell culture bag, which needs manual or related device to assist operation.

[0003] When collecting cell culture solution, the operator holds the cell culture bag to pour the liquid. With the outflow of the culture solution, the liquid in the cell culture bag gradually decreases, and the pressure gradually decreases. The operator needs to raise the hand to make the liquid flow out. This process needs to constantly adjust the position of the hand, which is not convenient and has low efficiency. In the late stage of liquid discharge, a small amount of cell culture solution may be retained in the culture bag and not easily discharged, causing waste. The existing cell culture solution collection device is mostly used to squeeze the cell bag to discharge the liquid, which may cause extrusion to the cells and affect the activity of the cells. Moreover, when using these devices, the flow rate of the cell culture solution cannot be controlled, and the liquid may be easily spilled and contaminated if not operated properly. UTILITY MODEL CONTENTS

[0004] The utility model solves the above technical problems, and provides a cell culture solution collection device capable of controlling flow rate, which comprises:

[0005] The support comprises a base, two support rods fixedly connected to the two sides of the base, and two lobster buckles fixedly connected to the top of the two support rods.

[0006] The liquid discharge rotating mechanism comprises a clamp, a rotating rod, a rotating gear, a silica gel gasket, a spring tab and a rotating handle. The rotating rod penetrates through the lobster buckle and is fixedly connected with the clamp and the rotating gear. The silica gel gasket is sleeved on the rotating rod and located between the lobster buckle and the rotating gear. The rotating handle is threadedly connected with the rotating rod. The spring tab is fixed to the right lobster buckle through a connecting block, and the thickness of the connecting block is the same as that of the silica gel gasket.

[0007] Further, the top of the support rod is provided with a hollow groove, and the lobster buckle is embedded in the hollow groove for fixation. The bottom of the base is provided with a silica gel pad.

[0008] Further, the lobster clasp comprises a clasp body, a clasp core and a first torsion spring; the clasp body is provided with a first limiting block and a first circular hole, the clasp core is provided with a first positioning slot with an opening, a first sheath shaft and a first through hole; the front end of the first torsion spring is inserted into the first positioning slot, the rear end is abutted against the first limiting block, the middle part is sleeved on the first sheath shaft, and the clasp body and the clasp core are connected through the first screw passing through the first circular hole and the first through hole; the inner side of the clasp body and the clasp core is provided with a first silica gel strip.

[0009] Further, the spring push piece comprises a shell, a push piece and a second torsion spring; the shell is provided with a second limiting block and a second circular hole, the push piece is provided with a second positioning slot with an opening, a second sheath shaft and a second through hole; the front end of the second torsion spring is inserted into the second positioning slot, the rear end is abutted against the second limiting block, the middle part is sleeved on the second sheath shaft, and the shell and the push piece are connected through the second screw passing through the second circular hole and the second through hole; the upper surface and the bottom of the front end of the push piece are provided with a second silica gel strip.

[0010] Further, the clamp comprises two stainless steel sheets and a third torsion spring connecting the two; the stainless steel sheet is provided with a third circular hole, the rotating rod passes through the third torsion spring and the third circular hole and is fixed with the third torsion spring; the clamp is provided with a third silica gel strip at the clamping opening.

[0011] Further, the cell culture liquid collecting device with controllable flow rate further comprises: a flow rate regulator sleeved on the drainage pipe of the cell culture bag for adjusting the flow rate of the liquid; the flow rate regulator comprises a device body and a pulley; the device body is provided with a slide and a front and rear square hole, the center of the pulley is provided with a small roller with a fourth silica gel strip, and the small roller is slidably embedded in the slide.

[0012] Further, the base, the supporting rod, the rotating rod, the lobster clasp, the clamp, the rotating gear, the spring push piece, the rotating handle, the connecting block and the flow rate regulator are all made of stainless steel.

[0013] The technical scheme of the utility model, when in use, the cell culture bag is clamped by the clamp, the height of the cell culture bag is adjusted through the drainage rotating mechanism, the cell culture liquid is completely discharged due to gravity without being subjected to extrusion, unnecessary waste is reduced while the cell activity is ensured, the flow rate regulator is sleeved on the drainage pipe of the cell culture bag, the flow rate of the cell culture liquid discharge is controlled through the adjusting pulley, and pollution caused by spilling is avoided; the device can save manpower, is simple and convenient to operate, and is shorter in time and higher in efficiency compared with manual operation when discharging the cell culture liquid. BRIEF DESCRIPTION OF DRAWINGS

[0014] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced as follows. Obviously, the drawings in the following description only show some of the embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of the drawings shown.

[0015] Figure 1 The structure diagram of the cell culture solution collecting device with controllable flow rate of the present application;

[0016] Figure 2 The structure diagram of the support rod and lobster buckle fixed connection of the present application;

[0017] Figure 3 The structure diagram and use state diagram of the lobster buckle assembly of the present application;

[0018] Figure 4 The structure diagram of the drainage rotating mechanism assembly of the present application;

[0019] Figure 5 The structure diagram of the spring push piece and lobster buckle fixed connection of the present application;

[0020] Figure 6 The structure diagram and use state diagram of the spring push piece assembly of the present application;

[0021] Figure 7 The structure diagram of the clamp top view and rotating rod fixed connection of the present application;

[0022] Figure 8 The structure diagram of the flow rate regulator of the present application;

[0023] Figure 9 The structure diagram of the base, small roller, lobster buckle and spring push piece of the present application is enlarged;

[0024] Figure 10 The use state diagram of the cell culture solution collecting device with controllable flow rate of the present application.

[0025] BRIEF DESCRIPTION OF DRAWINGS: 1-flow rate regulator, 101-body, 102-pulley, 103-ramp, 104-square hole, 105-small roller; 3-supporting rod; 4-rotating rod; 5-lobster clasp, 501-clasp body, 502-clasp core, 503-first torsional spring, 504-first limiting block, 505-first circular hole, 506-first positioning groove, 507-first sheath shaft, 508-first through hole, 509-first torsional spring front end, 510-first positioning groove opening, 511-first torsional spring rear end, 512-first torsional spring middle part, 513-first screw; 6-clamp, 601-stainless steel sheet, 602-third torsional spring, 603-third circular hole; 7-rotating gear; 8-silicone rubber gasket; 9-spring tab, 901-housing, 902-tab, 903-second torsional spring, 904-second limiting block, 905-second circular hole, 906-second positioning groove, 907-second sheath shaft, 908-second through hole, 909-second torsional spring front end, 910-second positioning groove opening, 911-second torsional spring rear end, 912-second torsional spring middle part, 913-second screw; 10-rotating handle; 11-connection block; 12-cell culture bag; 13-drainage tube; 14-empty groove; 15-silicone rubber pad; 16-first silicone rubber strip; 17-second silicone rubber strip; 18-third silicone rubber strip; 19-fourth silicone rubber strip; 20-drainage tube cover. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the specification of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0027] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present application are only used to explain the relative positional relationship, movement condition, etc. between components in a certain specific posture (as shown in the drawings), and if the specific posture changes, the directional indications also change accordingly.

[0028] In addition, the description of "first", "second" and the like in the present application is only for the purpose of description, and cannot be understood as indicating or implying the relative importance of the indicated technical features or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "several", "a plurality of" is at least two, for example, two, three, etc., unless otherwise specifically limited.

[0029] In the utility model, unless another definite provision and limitation, the terms "connect", "fix" and the like should be broad sense understanding, for example, "fix" can be fixed connection, also can be detachable connection, or be integrated, can be mechanical connection, also can be electrical connection, can be direct connection, also can through the indirect connection of intermediate medium, can be two element internal communication or the interaction of two elements, unless another definite limitation, for the ordinary skill in the art, the specific meaning of the above terms in the utility model can be understood according to specific circumstances.

[0030] In addition, the technical solutions of various embodiments of the utility model can be combined with each other, but it must be based on that the ordinary skill in the art can be realized, when the combination of technical solutions appears mutual contradiction or cannot be realized, it should be considered that the combination of technical solutions does not exist, also not within the protection scope required by the utility model.

[0031] The utility model provides a cell culture solution collecting device of controllable flow rate, aims at improving the sensitivity and practicality of cell culture solution collecting device.

[0032] The cell culture solution collecting device of controllable flow rate proposed by the utility model will be described in the specific embodiments as follows:

[0033] In the technical scheme of the embodiment, as shown in Figures 1-8 A cell culture solution collecting device of controllable flow rate, characterized by comprising:

[0034] The support comprises a base 2, two support rods 3 fixedly connected to the two sides of the base 2, and two lobster buckles 5 fixedly connected to the top of the two support rods 3 respectively;

[0035] The liquid discharge rotating mechanism comprises a clamp 6, a rotating rod 4, a rotating gear 7, a silica gel gasket 8, a spring push piece 9 and a rotating handle 10; the rotating rod 4 penetrates through the lobster buckle 5 and is fixedly connected with the clamp 6 and the rotating gear 7, the silica gel gasket 8 is sleeved on the rotating rod 4 and located between the lobster buckle 5 and the rotating gear 7; the rotating handle 10 is threadedly connected with the rotating rod 4; the spring push piece 9 is fixed to the right lobster buckle 5 through a connecting block 11, and the thickness of the connecting block 11 is the same as that of the silica gel gasket 8.

[0036] Further, as shown in Figure 2 The top of the support rod 3 is provided with a hollow groove 14, and the lobster buckle 5 is embedded in the hollow groove 14 and fixed; the bottom of the base 2 is provided with a silica gel pad 15.

[0037] Further, as shown in Figure 3 , Figure 4As shown, the lobster clasp 5 includes a clasp body 501, a clasp core 502, and a first torsion spring 503; the clasp body 501 is provided with a first limiting block 504 and a first circular hole 505, the clasp core 502 is provided with a first positioning slot 506 with an opening, a first sheath shaft 507, and a first through hole 508; the front end 509 of the first torsion spring 503 is inserted into the first positioning slot 506, the rear end 511 abuts against the first limiting block 504, the middle part 512 is sleeved on the first sheath shaft 507, and the first clasp body 501 and the clasp core 502 are connected by the first screw 513 passing through the first circular hole 505 and the first through hole 508; the inner sides of the clasp body 501 and the clasp core 502 are provided with a first silica gel strip 16.

[0038] Further, as shown in Figure 5 , Figure 6 , Figure 9 , the spring tab 9 includes a shell 901, a tab 902, and a second torsion spring 903; the shell 901 is provided with a second limiting block 904 and a second circular hole 905, the tab 902 is provided with a second positioning slot 906 with an opening, a second sheath shaft 907, and a second through hole 908; the front end 909 of the second torsion spring 903 is inserted into the second positioning slot 906, the rear end 911 abuts against the second limiting block 904, the middle part 912 is sleeved on the second sheath shaft 907, and the shell 901 and the tab 902 are connected by the second screw 913 passing through the second circular hole 905 and the second through hole 908; the upper surface and the bottom of the front end of the tab 902 are provided with a second silica gel strip 17.

[0039] Further, as shown in Figure 7 , the clamp 6 includes two stainless steel sheets 601 and a third torsion spring 602 connecting the two; the stainless steel sheet 601 is provided with a third circular hole 603, the rotating rod 4 passes through the third torsion spring 602 and the third circular hole 603 and is fixed with the third torsion spring 602; the clamping opening of the clamp 6 is provided with a third silica gel strip 18.

[0040] Further, the cell culture liquid collection device with controllable flow rate further includes: a flow rate regulator 1 sleeved on the drainage tube 13 of the cell culture bag 12, for adjusting the flow rate of the liquid;

[0041] As shown in Figure 8 , the flow rate regulator 1 includes a body 101 and a pulley 102; the body 101 is provided with a slide 103 and a front and rear square hole 104, and the center of the pulley 102 is provided with a small roller 105 with a fourth silica gel strip 19, which is slidably embedded in the slide 103.

[0042] Further, the base 2, the supporting rod 3, the rotating rod 4, the lobster clasp 5, the clamp 6, the rotating gear 7, the spring tab 9, the rotating handle 10, the connecting block 11, and the flow rate regulator 1 are all made of stainless steel.

[0043] Working principle: as shown in Figure 10As shown, in use, the clamp 6 clamps the cell culture bag 12, the drainage tube 13 passes through the flow rate regulator 1, after the drainage tube cover 20 of the drainage tube 13 is screwed open, the cell culture solution flows out due to gravity, and the flow rate can be controlled by sliding the pulley 102 of the flow rate regulator 1 during the process; in the later stage of discharging, the liquid in the cell culture bag 12 gradually decreases, the pressure decreases, and the culture solution is not easy to flow out, for example Figure 10 As shown, the rotating handle 10 can be controlled to rotate clockwise, so that the rotating rod 4, the rotating gear 7 and the clamp 6 all rotate clockwise, so that the empty cell culture bag 12 above which there is no culture solution is wound on the clamp 6, the height of the remaining culture solution in the bag is increased, and the discharge is facilitated; for example Figure 6 As shown, when the rotating gear 7 rotates clockwise, an upward force is applied to the front end of the push piece 902, so that the front end of the push piece 902 is retracted and does not hinder the operation of the rotating gear 7; after the cell culture bag 12 is adjusted to an appropriate height, the operator can release the handle, if the remaining culture solution in the bag is too much and too heavy, the rotating gear 7 can be pulled to rotate in the opposite direction, at this time, the front end of the push piece 902 can be clamped in the tooth gap of the rotating gear 7, preventing the empty cell culture bag 12 wound on the clamp from falling off, causing the height of the remaining culture solution to be too low and not easy to flow out; after the cell culture solution is collected, the rear end of the push piece 902 can be pressed downward, so that the front end of the push piece 902 is retracted and cannot touch the rotating gear 7, the rotating handle 10 is rotated counterclockwise, the empty cell culture bag 12 wound on the clamp 6 falls off, the clamp 6 is pressed to release the empty cell culture bag 12, and then the empty cell culture bag 12 can be handled.

[0044] The above is only a preferred specific embodiment of the present application, but the protection scope of the present application is not limited thereto, any person skilled in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A cell culture medium collection device with controllable flow rate, characterized in that, The utility model relates to a cell culture bag liquid flow speed regulator, including: Support frame, including base (2), two support rods (3) fixedly connected on both sides of base (2) and two lobster buckles (5) fixedly connected on the top of two support rods (3) respectively; Liquid discharging rotating mechanism, including clamp (6), rotating rod (4), rotating gear (7), silica gel washer (8), spring switch (9) and rotating handle (10), rotating rod (4) penetrates lobster buckle (5) and is fixedly connected with clamp (6) and rotating gear (7), silica gel washer (8) is sleeved on rotating rod (4) and is located between lobster buckle (5) and rotating gear (7), rotating handle (10) is threadedly connected with rotating rod (4), spring switch (9) is fixed to right side lobster buckle (5) through connecting block (11), and the thickness of connecting block (11) is same with silica gel washer (8).

2. The flow rate controllable cell culture fluid collection device according to claim 1, wherein, Support rod (3) top is equipped with empty slot (14), and lobster buckle (5) is embedded in empty slot (14) and is fixed, and base (2) bottom is equipped with silica gel pad (15).

3. The flow rate controllable cell culture fluid collection device of claim 1, wherein, Lobster buckle (5) includes buckle body (501), buckle core (502) and first torsional spring (503), buckle body (501) is equipped with first limit block (504) and first round hole (505), buckle core (502) is equipped with first positioning slot (506) with opening, first sheath shaft (507) and first through hole (508), the front end (509) of first torsional spring (503) is inserted into first positioning slot (506), rear end (511) is abutted to first limit block (504), middle part (512) is sleeved on first sheath shaft (507), and is connected buckle body (501) and buckle core (502) through first screw (513) passing through first round hole (505) and first through hole (508), the inboard of buckle body (501) and buckle core (502) is equipped with first silica gel strip (16).

4. The flow rate controllable cell culture fluid collection device of claim 1, wherein, Spring switch (9) includes shell (901), switch (902) and second torsional spring (903), shell (901) is equipped with second limit block (904) and second round hole (905), switch (902) is equipped with second positioning slot (906) with opening, second sheath shaft (907) and second through hole (908), the front end (909) of second torsional spring (903) is inserted into second positioning slot (906), rear end (911) is abutted to second limit block (904), middle part (912) is sleeved on second sheath shaft (907), and is connected shell (901) and switch (902) through second screw (913) passing through second round hole (905) and second through hole (908), the upper surface and bottom of switch (902) front end are equipped with second silica gel strip (17).

5. The flow rate controllable cell culture fluid collection apparatus of claim 1, wherein, Clamp (6) includes two stainless steel sheets (601) and third torsional spring (602) connecting both, stainless steel sheet (601) is equipped with third round hole (603), rotating rod (4) passes through third torsional spring (602) and third round hole (603) and is fixed with third torsional spring (602), and the mouth of clamp (6) is equipped with third silica gel strip (18).

6. The flow rate controllable cell culture fluid collection apparatus of claim 1, wherein, Still include: flow speed regulator (1) is sleeved on the drainage pipe (13) of cell culture bag (12), is used for adjusting liquid flow speed; The flow rate regulator (1) comprises a body (101) and a pulley (102); the body (101) is provided with a slide (103) and front and rear square holes (104), and the pulley (102) is provided with a small roller (105) with a fourth silica gel strip (19) in the center, which is slidably embedded in the slide (103).

7. The flow rate controllable cell culture fluid collection device of claim 1, wherein, The base (2), the supporting rod (3), the rotating rod (4), the lobster buckle (5), the clamp (6), the rotating gear (7), the spring push piece (9), the rotating handle (10), the connecting block (11) and the flow rate regulator (1) are all made of stainless steel.

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