Bioreactor for culturing calculus bovis in vitro

Abstract

The utility model relates to the technical field of calculus bovis cultivation, and discloses a bioreactor for in-vitro cultivation of calculus bovis, which comprises a reactor body, a material uniformizing mechanism arranged in an inner cavity of the reactor body, a plurality of groups of oxygen supply tanks arranged at the upper end of the reactor body, and a first electromagnetic valve arranged at the lower end of each group of oxygen supply tank, an oxygen supply mechanism is arranged at the bottom of the inner cavity of the reactor body and is arranged below the refining mechanism; through cooperation of the oxygen supply mechanism, the reactor body and the material uniformizing mechanism, oxygen can be uniformly introduced into the bottom of the inner cavity of the reactor body through the oxygen supply mechanism, so that the contact range between the oxygen and cells is expanded, and meanwhile, under the action of the material uniformizing mechanism, the oxygen can flow in the reactor body along with bile, so that the bile is uniformly dispersed. Therefore, the oxygen can be uniformly supplied to the cells, and the mixing uniformity of the bile and various raw materials for promoting cell differentiation is improved, so that the cultivation efficiency of the calculus bovis is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of cultivating technology of ox gall, concretely is a bioreactor for cultivating ox gall in vitro. BACKGROUND

[0002] Ox gall is a traditional Chinese medicine, which is a gallstone produced by the gallbladder of a cow. Its surface is golden yellow or yellowish brown, delicate and lustrous. It has a slightly cool nature and can be used for some heat diseases. It has the effects of relieving fever and detoxification and calming. Natural ox gall is very rare and expensive. Therefore, people have developed ox gall cultivated in vitro. This kind of ox gall is mainly made of fresh cow bile as mother liquor, and deoxycholic acid, cholic acid, and calcium complex bilirubin are added.

[0003] When cultivating ox gall in vitro, cow bile and added deoxycholic acid, cholic acid, and calcium complex bilirubin are usually needed to promote cell differentiation and the generation of ox gall. However, oxygen needs to be added to the reactor simulating the in vivo environment of the cow during the generation process to supply oxygen to the cells. The traditional oxygen supply structure does not uniformly supply oxygen to the cells in the reactor, resulting in uneven oxygen concentration in the upper and lower layers, which further affects the cultivation effect of ox gall. Therefore, we need to propose a bioreactor for cultivating ox gall in vitro. SUMMARY

[0004] The utility model aims to provide a bioreactor for cultivating ox gall in vitro, which improves the oxygen supply range in the reactor, increases the contact range of oxygen and cells, and thus improves the oxygen supply effect of the cells and the cultivation efficiency of ox gall, to solve the problems raised in the background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a bioreactor for cultivating ox gall in vitro, comprising a reactor body, a uniform material mechanism is arranged in the inner cavity of the reactor body, a plurality of groups of feeding tanks are arranged at the upper end of the reactor body, a first electromagnetic valve is arranged at the lower end of each group of feeding tanks, an oxygen supply mechanism is arranged at the bottom of the inner cavity of the reactor body, the oxygen supply mechanism is arranged below the uniform material mechanism, and a discharge mechanism is arranged at the lower end of the reactor body.

[0006] The oxygen supply mechanism comprises an annular oxygen supply pipe and a plurality of groups of supporting rods, the supporting rods are fixedly connected to the lower end of the annular oxygen supply pipe, a gas source connecting pipe penetrating through the reactor body is communicated with the annular oxygen supply pipe, a plurality of groups of oxygen supply holes are arranged at the lower end of the annular oxygen supply pipe, and the oxygen supply holes are arranged in a circular array.

[0007] Preferably, the uniform material mechanism comprises a motor and a rotating shaft, the upper end of the rotating shaft penetrates through the reactor body and is driven by the motor, and a turnover blade and a stirring piece are arranged at the lower end of the rotating shaft.

[0008] Preferably, the stirring piece comprises a fixed ring fixedly connected to the end of the rotating shaft, the outer side of the fixed ring is symmetrically fixedly connected with horizontal rods, the lower middle part of the lower end of the two groups of horizontal rods is fixedly connected with first scraping rods, and the end of the lower end of the two groups of horizontal rods away from the rotating shaft is fixedly connected with second scraping rods.

[0009] Preferably, the inner tube is provided with a plurality of groups of supporting rods at the lower end, a connecting channel is arranged between two adjacent groups of supporting rods, the turning blade is arranged in the inner cavity of the inner tube, the first scraping rod is slidingly arranged on the outer wall of the inner tube, and the second scraping rod is slidingly arranged on the inner wall of the reactor body.

[0010] Preferably, two groups of connecting rods are fixedly connected between the first scraping rod and the second scraping rod, a drainage sheet is fixedly connected to the middle part of the two groups of connecting rods, and the drainage sheet is arranged in an inclined manner.

[0011] Preferably, the outer side of the reactor body is provided with a controller and a feeding pipe, and the lower end of the reactor body is provided with a supporting chassis.

[0012] Preferably, the discharging mechanism comprises a bottom pipe fixedly connected to the lower end of the reactor body, a discharging pipe is arranged on the outer side of the bottom pipe, a gas cylinder is arranged at the lower end of the bottom pipe, and a sealing piston is arranged at the telescopic end of the gas cylinder and penetrates the bottom pipe.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] The utility model mainly through the cooperation between oxygen supply mechanism, reactor body and uniform material mechanism, through oxygen supply mechanism can pass into the oxygen even the reactor body inner chamber bottom, thereby expand the contact range between oxygen and cell, simultaneously under the action of uniform material mechanism, make oxygen can flow along with bile in the reactor body, further make oxygen can even oxygen supply to cell, improve bile and various raw materials that promote cell differentiation mix evenness, thereby improve the cultivation efficiency of calculus bovis. BRIEF DESCRIPTION OF DRAWINGS

[0015] Fig. 1 It is whole structure schematic diagram of the utility model;

[0016] Fig. 2 It is internal structure schematic diagram of reactor body of the utility model;

[0017] Fig. 3 It is stirring piece structure schematic diagram of the utility model.

[0018] In the diagram: 1. Reactor body; 2. Feeding mechanism; 21. Motor; 22. Rotating shaft; 23. Turning blades; 24. Stirring component; 241. Crossbar; 242. First scraper; 243. Second scraper; 244. Connecting rod; 245. Guide plate; 246. Fixing ring; 3. Oxygen supply tank; 4. First solenoid valve; 5. Oxygen supply mechanism; 51. Gas source connection pipe; 52. Annular oxygen supply pipe; 53. Support rod; 54. Oxygen supply hole; 6. Discharge mechanism; 61. Bottom pipe; 62. Discharge pipe; 63. Cylinder; 64. Sealing piston; 7. Support base frame; 8. Controller; 9. Feed pipe; 10. Inner pipe; 11. Support rod; 12. Connecting channel. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Please see Figs. 1-3 This utility model provides a technical solution: a bioreactor for in vitro culture of bezoar, including a reactor body 1, a uniform feeding mechanism 2 is provided in the inner cavity of the reactor body 1, multiple sets of feeding tanks 3 are provided at the upper end of the reactor body 1, a first solenoid valve 4 is provided at the lower end of each set of feeding tanks 3, an oxygen supply mechanism 5 is provided at the bottom of the inner cavity of the reactor body 1, the oxygen supply mechanism 5 is located below the uniform feeding mechanism 2, and a discharge mechanism 6 is provided at the lower end of the reactor body 1.

[0021] The oxygen supply mechanism 5 includes an annular oxygen supply pipe 52 and multiple sets of support rods 53. The multiple sets of support rods 53 are all fixedly connected to the lower end of the annular oxygen supply pipe 52. The annular oxygen supply pipe 52 is connected to a gas source connection pipe 51 that penetrates the reactor body 1. Multiple sets of oxygen supply holes 54 are opened at the lower end of the annular oxygen supply pipe 52. The multiple sets of oxygen supply holes 54 are arranged in a circular array.

[0022] The feeding mechanism 2 includes a motor 21 and a rotating shaft 22. The upper end of the rotating shaft 22 passes through the reactor body 1 and is driven by the motor 21. The lower end of the rotating shaft 22 is provided with a turning blade 23 and a stirring element 24. The motor 21 drives the rotating shaft 22 to drive the turning blade 23 to draw bile and other nutrient materials at the bottom of the reactor body 1 upward and gather oxygen towards the middle, so that the oxygen comes into contact with the cells in the bile and then flows upward. Under the action of the stirring element 24, it flows to the bottom of the inner cavity of the reactor body 1, improving the oxygen circulation effect, thereby simulating the physiological environment in the body of cattle and improving the cultivation effect of bezoar.

[0023] The stirring piece 24 comprises a fixed ring 246 fixedly connected to the upper end of the rotating shaft 22, and a horizontal rod 241 is fixedly connected to the outer side of the fixed ring 246 in a symmetrical manner, and a first scraping rod 242 is fixedly connected to the middle of the lower end of the two groups of horizontal rods 241, and a second scraping rod 243 is fixedly connected to the end of the lower end of the two groups of horizontal rods 241 away from the rotating shaft 22, and the first scraping rod 242 and the second scraping rod 243 can avoid the attachment of the material to the inner wall of the inner tube 10 and the inner wall of the reactor body 1, and ensure the uniform distribution of the material in the inner cavity of the reactor body 1, and avoid local accumulation or uneven stirring.

[0024] The inner cavity of the reactor body 1 is provided with the inner tube 10, the lower end of the inner tube 10 is provided with a plurality of groups of support rods 11, the adjacent two groups of support rods 11 are provided with a connecting channel 12, the material turning blade 23 is arranged in the inner cavity of the inner tube 10, the first scraping rod 242 is slidingly arranged on the outer wall of the inner tube 10, and the second scraping rod 243 is slidingly arranged on the inner wall of the reactor body 1.

[0025] The first scraping rod 242 and the second scraping rod 243 are fixedly connected with two groups of connecting rods 244, the middle of the two groups of connecting rods 244 is fixedly connected with a flow guide piece 245, the flow guide piece 245 is arranged in an inclined manner, and the flow guide piece 245 guides the material to different areas in the inner cavity of the reactor body 1 in the rotating process, so that the material is fully mixed and reacted.

[0026] The outer side of the reactor body 1 is provided with a controller 8 and a feeding pipe 9, and the lower end of the reactor body 1 is provided with a supporting chassis 7, the operation of each mechanism is conveniently controlled through the controller 8, so that the automation degree in the cultivation process is improved.

[0027] The discharging mechanism 6 comprises a bottom pipe 61 fixedly connected to the lower end of the reactor body 1, the outer side of the bottom pipe 61 is provided with a discharging pipe 62, the lower end of the bottom pipe 61 is provided with a pneumatic cylinder 63, the telescopic end of the pneumatic cylinder 63 penetrates through the bottom pipe 61 and is provided with a sealing piston 64, the position of the sealing piston 64 is conveniently adjusted through the pneumatic cylinder 63, so that the cultivated bovine bezoar in the reactor body 1 is conveniently discharged.

[0028] In use, bovine bile is introduced into the reactor body 1 through the feeding pipe 9, different components promoting cell differentiation are introduced through the plurality of feeding tanks 3, the gas source of the oxygen supply mechanism 5 is opened, so that oxygen is introduced into the annular oxygen supply pipe 52 through the gas source connecting pipe 51, and is discharged through the uniformly distributed oxygen supply holes 54, so that the oxygen supply range is increased, and the oxygen can be well absorbed by the cells, at the same time, the motor 21 drives the rotating shaft 22 to drive the stirring piece 24 and the material turning blade 23 to rotate, so that the bile and the material and oxygen enter the inner tube 10 through the connecting channel 12, and the material is lifted from the lower end of the inner tube 10 to the upper end of the inner tube 10, and is then dispersed into the inner cavity of the reactor body 1, and the oxygen is dispersed under the action of the flow guide piece 245, so that the uniformity of oxygen dispersion is improved, the uniformity of oxygen supply to the cells is ensured, and the efficiency of bovine bezoar cultivation is improved.

[0029] While the embodiments of the present application have been illustrated and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the spirit and scope of the application, which is defined by the appended claims and their equivalents.

Claims

1. A bioreactor for in vitro culture of bezoar, comprising a reactor body (1), characterized in that: The reactor body (1) is provided with a uniform feeding mechanism (2) in its inner cavity. Multiple sets of oxygen supply tanks (3) are provided at the upper end of the reactor body (1). Each set of oxygen supply tanks (3) is provided with a first solenoid valve (4) at its lower end. An oxygen supply mechanism (5) is provided at the bottom of the inner cavity of the reactor body (1). The oxygen supply mechanism (5) is located below the uniform feeding mechanism (2). A discharge mechanism (6) is provided at the lower end of the reactor body (1). The oxygen supply mechanism (5) includes an annular oxygen supply pipe (52) and multiple sets of support rods (53). The multiple sets of support rods (53) are fixedly connected to the lower end of the annular oxygen supply pipe (52). The annular oxygen supply pipe (52) is connected to a gas source connection pipe (51) that penetrates the reactor body (1). The lower end of the annular oxygen supply pipe (52) is provided with multiple sets of oxygen supply holes (54), which are arranged in a circular array.

2. The bioreactor for in vitro culture of bezoar according to claim 1, characterized in that: The material leveling mechanism (2) includes a motor (21) and a rotating shaft (22). The upper end of the rotating shaft (22) passes through the reactor body (1) and is driven by the motor (21). The lower end of the rotating shaft (22) is provided with a material turning blade (23) and a stirring component (24).

3. The bioreactor for in vitro culture of bezoar according to claim 2, characterized in that: The stirring component (24) includes a fixed ring (246) fixedly connected to the upper end of the rotating shaft (22). Crossbars (241) are symmetrically fixedly connected to the outer side of the fixed ring (246). A first scraper (242) is fixedly connected to the middle of the lower end of the two sets of crossbars (241). A second scraper (243) is fixedly connected to the lower end of the two sets of crossbars (241) away from the rotating shaft (22).

4. A bioreactor for in vitro culture of bezoar according to claim 3, characterized in that: The reactor body (1) has an inner tube (10) in its inner cavity. The lower end of the inner tube (10) is provided with multiple sets of support rods (11). A connecting channel (12) is provided between two adjacent sets of support rods (11). The material turning blade (23) is provided in the inner cavity of the inner tube (10). The first scraper (242) is slidably disposed on the outer wall of the inner tube (10), and the second scraper (243) is slidably disposed on the inner wall of the reactor body (1).

5. A bioreactor for in vitro culture of bezoar according to claim 4, characterized in that: Two sets of connecting rods (244) are fixedly connected between the first scraper (242) and the second scraper (243). A diversion plate (245) is fixedly connected to the middle of each of the two sets of connecting rods (244), and the diversion plate (245) is inclined.

6. A bioreactor for in vitro culture of bezoar according to claim 1, characterized in that: The reactor body (1) is equipped with a controller (8) and a feed pipe (9) on its outer side, and a support frame (7) is installed at the lower end of the reactor body (1).

7. A bioreactor for in vitro culture of bezoar according to claim 1, characterized in that: The discharge mechanism (6) includes a bottom pipe (61) fixedly connected to the lower end of the reactor body (1), a discharge pipe (62) is provided on the outside of the bottom pipe (61), and a cylinder (63) is provided at the lower end of the bottom pipe (61). The telescopic end of the cylinder (63) passes through the bottom pipe (61) and is equipped with a sealing piston (64).

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