Horizontal shaft stirring reactor for preparing mesenchymal stem cell spheres

Abstract

The utility model discloses a horizontal shaft stirring reactor for preparing mesenchymal stem cell spheres, and relates to the technical field of mesenchymal stem cell sphere preparation, the horizontal shaft stirring reactor comprises a container and a stirring device, the container is used for containing a culture solution for balling stem cells, the stirring device is arranged in the container, the top of the container is provided with an opening, and a top cover capable of opening the opening is fixed at the top of the container. The stirring device comprises a stirring shaft which is transversely arranged and a plurality of stirring blades which are transversely arranged around the stirring shaft and are arranged in the container, the stirring shaft is driven by a driving assembly which is arranged outside the container to rotate, the driving assembly drives the stirring shaft to rotate in a magnetic coupling mode, and the driving shaft drives the stirring blades to stir the culture solution in the container; the balling of the mesenchymal stem cells is promoted. According to the utility model, a horizontal stirring structure is innovated, the flow field distribution of the reactor is optimized, the problem of non-uniform shearing force in a cell ball culture process is effectively solved, and a key technical support is provided for industrial production of high-quality stem cell balls.

Description

TECHNICAL FIELD

[0001] The utility model relates to mesenchymal stem cell ball preparation technical field, specifically a kind of horizontal shaft stirring reactor for mesenchymal stem cell ball preparation. BACKGROUND

[0002] In the modern biomedical field, mesenchymal stem cells (MSC) show great application value in tissue engineering, regenerative medicine and cell therapy due to their multi-directional differentiation potential and immune regulation function. Stem cell ball culture technology can effectively maintain stem cell stemness and promote intercellular signal transmission, which is a key means for large-scale production of high-quality stem cells. At present, stem cell ball preparation technology based on bioreactor has become a research hotspot, but the existing device still has significant deficiencies in cell aggregation efficiency, microenvironment regulation and engineering scale-up.

[0003] Traditional stirring bioreactor mostly adopts vertical shaft stirring structure (such as propeller type, turbine type stirring paddle), and the shear force distribution is uneven, which easily leads to cell damage on the surface of stem cell ball or ball breakage, especially in high cell density culture, the problem of cell apoptosis caused by shear force is more prominent. At the same time, the vortex formed by vertical stirring easily causes mass transfer gradient of nutrients, oxygen and signal molecules in the culture system, leading to uneven size of stem cell ball, core necrosis and other phenomena, which seriously affects the uniformity of cell ball quality. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a kind of horizontal shaft stirring reactor for mesenchymal stem cell ball preparation to solve the problems raised in the above background technology.

[0005] To achieve the above object, the utility model provides the following technical scheme:

[0006] The horizontal shaft stirring reactor for mesenchymal stem cell ball preparation includes a container for containing culture solution for stem cell ball formation and a stirring device arranged in the container, the top of the container is open, and a top cover that can open the opening is fixed to the top of the container, the stirring device includes a transversely arranged stirring shaft and a plurality of stirring blades arranged around the stirring shaft transversely in the container, the stirring shaft is driven to rotate by a driving assembly arranged outside the container, the driving assembly drives the stirring shaft to rotate by magnetic coupling, the stirring shaft drives the stirring blades to stir the culture solution in the container, and promotes the formation of mesenchymal stem cell ball.

[0007] Compared with the prior art, the utility model has the advantages that:

[0008] The utility model effectively solves the problem of uneven shear force in the process of cell ball culture by innovating horizontal stirring structure and optimizing reactor flow field distribution, which provides key technical support for the industrialized production of high-quality stem cell ball. BRIEF DESCRIPTION OF DRAWINGS

[0009] Fig. 1 Structure diagram of horizontal shaft stirring reactor for mesenchymal stem cell sphere preparation.

[0010] Fig. 2 Exploded view of horizontal shaft stirring reactor for mesenchymal stem cell sphere preparation.

[0011] Fig. 3 Structure diagram of stirring device in horizontal shaft stirring reactor for mesenchymal stem cell sphere preparation.

[0012] Fig. 4 Structure diagram of mounting bracket in horizontal shaft stirring reactor for mesenchymal stem cell sphere preparation.

[0013] Fig. 5 Structure diagram of active magnetic ring and protective cover in horizontal shaft stirring reactor for mesenchymal stem cell sphere preparation.

[0014] Fig. 6 Structure diagram of container interior in horizontal shaft stirring reactor for mesenchymal stem cell sphere preparation. DETAILED DESCRIPTION

[0015] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0016] Please refer to Figs. 1-6 In the embodiments of the present application, the horizontal shaft stirring reactor for mesenchymal stem cell sphere preparation comprises a container 1 for containing culture solution for stem cell sphere formation and a stirring device 5 arranged in the container 1. The top of the container 1 is provided with an opening, and a top cover 2 capable of opening the opening is fixed to the top of the container 1. The stirring device 5 comprises a stirring shaft 51 arranged transversely and a plurality of stirring blades 52 arranged transversely around the stirring shaft 51 in the container 1. The stirring shaft 51 is driven to rotate by a driving assembly 3 arranged outside the container 1. The driving assembly 3 drives the stirring shaft 51 to rotate by magnetic coupling. The stirring shaft 51 drives the stirring blades 52 to stir the culture solution in the container 1, thereby promoting the formation of mesenchymal stem cell spheres.

[0017] The utility model discloses through the innovation level stirring structure, optimization reactor flow field distribution, effectively solved the problem of uneven shear force in the cell ball culture process, provides the key technical support for the industrialization production of high quality stem cell ball, and through the setting of the stirring mode of horizontal stirring shaft structure, avoided a series of problems caused by the vortex of vertical stirring in the prior art.

[0018] In addition, the utility model discloses through the magnetic coupling mode drive stirring device 5, the both sides of whole container 1 are completely sealed, do not have the position of opening, can effectively avoid the leakage problem of transmission shaft penetration container wall in the prior art.

[0019] The stirring blade 52 is circumferentially distributed around the stirring shaft 51 in multiple different diameters, in order to improve the uniformity in the stirring process, the stirring blades 52 in the same radial direction are uniformly distributed, and in specific setting, the stirring blade is a horizontally arranged rod, both ends of the rod are connected to a plurality of connecting rods arranged radially along the stirring shaft 51, and the end of the connecting rod is fixedly connected with the stirring shaft 51.

[0020] The stirring device 5 further comprises a driven magnetic ring 53 coaxially arranged with the stirring shaft 51, the driven magnetic ring 53 rotates synchronously with the stirring shaft 51 and the stirring blade 52, the driving assembly 3 comprises a driving magnetic ring 31 arranged outside the container 1 and a driving motor 33 arranged outside the container 1, the driving motor 33 drives the driving magnetic ring 31 to rotate, the driving magnetic ring 31 drives the driven magnetic ring 53 to rotate through the side wall of the container 1, so as to drive the stirring shaft 51 and the stirring blade 52 to stir the culture solution in the container 1.

[0021] The magnetic poles of the driven magnetic ring 53 and the driving magnetic ring 31 are opposite, and when the driving magnetic ring 31 rotates, the driven magnetic ring 53 can be driven to rotate, so that the power transmission is realized.

[0022] The inside of the driving magnetic ring 31 is fixedly connected with an inner ring 311 arranged coaxially through a connecting rod, and the container 1 further comprises a protective cover 32 covering the outer wall of the container 1, the protective cover 32 is rotatably connected with a driving shaft 321 arranged coaxially with the protective cover 32, the driving shaft 321 is coaxially arranged through the inside of the inner ring 311 and is fixedly connected with the inner ring 311, and the driving shaft 321 is fixedly connected with the driving shaft of the driving motor 33, so that the driving magnetic ring 31 is driven to rotate by the driving motor 33 through the driving shaft 321 and the inner ring 311.

[0023] In specific implementation, the pin can penetrate the inner ring 311 and the driving shaft 321, so that the two rotate synchronously, and the driving shaft 321 is synchronously rotated with the output shaft of the driving motor 33 through the key connection.

[0024] The outer edge of the protective cover 32 is fixedly connected with a mounting ring 322 for fixing connection with the container 1.

[0025] In the specific installation, a plurality of studs are fixed on the outer wall of the container 1 and pass through the mounting ring 322, nuts are threadedly connected on the studs, and the mounting ring 322 is pressed on the outer wall of the container 1 by the nuts to be fixed, so that the sealing property of the container 1 is ensured.

[0026] The stirring device 5 is mounted in the container 1 through the two mounting supports 4 symmetrically arranged relative to the stirring device 5.

[0027] The mounting support 4 comprises an n-shaped support vertically arranged, an installation slot 11 with only the top being open is fixed on the inner wall of the container 1 and perpendicular to the stirring shaft 51 of the stirring device 5, one vertical leg of the n-shaped support is inserted into the installation slot 11 from top to bottom, and a fixing shaft 42 is fixed on the other vertical leg of the n-shaped support and can be inserted into the stirring shaft 51, the fixing shafts 42 of the two mounting supports 4 on the two sides of the stirring device 5 are inserted into the stirring shaft 51 from the two ends of the stirring shaft 51, so that the stirring shaft 51 is mounted.

[0028] The distance between the two legs of the n-shaped support is the same as the wall thickness of the installation slot 11 inside the n-shaped support.

[0029] In order to facilitate the insertion of the mounting support 4 into the installation slot 11, a guide inclined surface 12 that opens outward can be arranged at the slot opening of the top of the installation slot 11, so that the installation difficulty of the mounting support 4 is reduced.

[0030] The top of the mounting support 4 is flush with the bottom surface of the top cover 2 fixed on the top of the container 1, when the top cover 2 is fixed on the top of the container 1, the mounting support 4 is pressed in the installation slot 11 to be fixed, and after the stirring device 5 is installed, the mounting support 4 can be stable and not shaken, so that the stability of the installation of the stirring device 5 is improved.

[0031] It should be noted that the driving motor 33 in the utility model can be a three-phase vertical motor with a model number of 80M1-4, and is powered by a municipal power supply system, and the speed, direction and other data in the working process can be controlled through a special controller, which is a mature technology, and will not be described here. In addition, in order to ensure that the stirring device 5 in the container 1 has a proper speed, a speed reducer can also be arranged between the driving motor and the driving shaft 321; this technology is easily thought of by a person skilled in the art, and will not be described in detail in this application.

[0032] Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part of the technical features, any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the utility model shall be included in the protection scope of the utility model, and the contents not described in detail in the specification are all the existing technology known to a person skilled in the art.

Claims

1. A horizontal axis stirred reactor for mesenchymal stem cell sphere preparation, characterized by, The utility model provides a container (1) and stirring device (5) for containing culture solution for stem cell spheroid, and the container (1) top is provided with an opening, and the top of container (1) is fixed with the top cover (2) that can open the opening, and the stirring device (5) includes transversely arranged stirring shaft (51) and multiple stirring blades (52) arranged in the container (1) and transversely arranged around the stirring shaft (51), the stirring shaft (51) is driven to rotate by the drive assembly (3) arranged outside the container (1), the drive assembly (3) drives the stirring shaft (51) to rotate by the mode of magnetic coupling, and the stirring shaft (51) drives the stirring blade (52) to stir the culture solution in the container (1), and promotes mesenchymal stem cell spheroid.

2. The horizontal-axis stirring reactor for mesenchymal stem cell sphere preparation according to claim 1, wherein The stirring blades (52) are distributed on multiple concentric circles with different diameters around the stirring shaft (51).

3. The horizontal-axis stirring reactor for mesenchymal stem cell sphere preparation according to claim 1, wherein The stirring device (5) further comprises a driven magnetic ring (53) coaxially arranged with the stirring shaft (51), the driven magnetic ring (53) rotates synchronously with the stirring shaft (51) and the stirring blades (52), the drive assembly (3) comprises a driving magnetic ring (31) arranged outside the container (1) and a drive motor (33) arranged outside the container (1), the drive motor (33) drives the driving magnetic ring (31) to rotate, and the driving magnetic ring (31) drives the driven magnetic ring (53) to rotate by the mode of magnetic coupling through the side wall of the container (1), so as to drive the stirring shaft (51) and the stirring blades (52) to stir the culture solution in the container (1).

4. The horizontal-axis stirring reactor for mesenchymal stem cell sphere preparation according to claim 3, wherein The inside of the driving magnetic ring (31) is fixed with an inner ring (311) coaxially arranged through a connecting rod, and the container (1) is further provided with a protective cover (32) covering the outer wall of the container (1) outside the driving magnetic ring (31), the protective cover (32) is rotatably connected with a driving shaft (321) coaxially arranged with the protective cover (32), the driving shaft (321) coaxially penetrates the inside of the inner ring (311) and is fixedly connected with the inner ring (311), and the driving shaft (321) is fixedly connected with the driving shaft of the drive motor (33), so that the driving magnetic ring (31) is driven to rotate by the drive motor (33) through the driving shaft (321) and the inner ring (311).

5. The horizontal-axis stirring reactor for mesenchymal stem cell sphere preparation according to claim 4, wherein The outer edge of the protective cover (32) is fixed with a mounting ring (322) for fixed connection with the container (1).

6. The horizontal-axis stirring reactor for mesenchymal stem cell sphere preparation according to claim 5, wherein A plurality of studs penetrating the mounting ring (322) are fixed on the outer wall of the container (1), and nuts are threadedly connected on the studs to fix the mounting ring (322) on the outer wall of the container (1).

7. The horizontal-axis stirring reactor for mesenchymal stem cell sphere preparation according to claim 1, wherein The stirring device (5) is installed in the container (1) through two mounting brackets (4) symmetrically arranged with respect to the stirring device (5).

8. The horizontal-axis stirring reactor for mesenchymal stem cell sphere preparation according to claim 7, wherein The mounting bracket (4) comprises a vertically arranged n-shaped bracket, an open-top mounting groove (11) is fixed on the inner wall of the two sides of the container (1) perpendicular to the stirring shaft (51) of the stirring device (5), one vertical leg of the n-shaped bracket is inserted into the mounting groove (11) from top to bottom, the other vertical leg of the n-shaped bracket is fixed with a fixing shaft (42) which can be inserted into the stirring shaft (51), the fixing shaft (42) of the mounting bracket (4) on both sides of the stirring device (5) is inserted into the stirring shaft (51) from both ends of the stirring shaft (51), so as to realize the installation of the stirring shaft (51).

9. The horizontal-axis stirring reactor for mesenchymal stem cell sphere preparation according to claim 8, wherein The distance between the two legs of the n-shaped bracket is the same as the wall thickness of the mounting groove (11) inside the n-shaped bracket.

10. The horizontal-axis stirring reactor for mesenchymal stem cell sphere preparation according to claim 8, wherein The top of the mounting bracket (4) is flush with the bottom surface of the top cover (2) fixed on the top of the container (1), when the top cover (2) is fixed on the top of the container (1), the mounting bracket (4) is pressed in the mounting groove (11) to be fixed.

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