Manual 96-hole pipettor

Abstract

The utility model discloses a manual 96-hole pipettor, and relates to the field of pipettes. The manual 96-hole pipettor comprises a pipette body, and further comprises a pipetting negative pressure bin which is in threaded connection with a suction end of the pipette body; according to the pipettor, 96 samples can be added at one time, so that the whole sample adding time is greatly shortened, the experiment process is greatly accelerated, the experiment efficiency is remarkably improved, and as the required reagent can be added at one time, the pollution risk caused by frequent operation in the repeated sample adding process is avoided; according to the method, the purity of experimental samples and the accuracy of experimental results are guaranteed, the characteristic that 96 samples are processed through one-time operation is achieved, the frequency of manual operation is reduced, workers can complete more experimental tasks within the same time, the overall working efficiency is effectively improved, meanwhile, the labor cost is reduced due to reduction of human input, and the working efficiency is improved. The method has obvious advantages in large-scale experiments or daily detection work.

Description

Technical Field

[0001] This utility model belongs to the field of pipette technology, specifically, it relates to a manual 96-well pipette. Background Technology

[0002] In modern scientific research fields such as biology, medicine, and chemistry, it is often necessary to precisely transfer and add trace amounts of liquid. 96-well plates, as a commonly used experimental consumable, are widely used in high-throughput screening, drug development, gene detection, cell culture and other experiments because they can process multiple samples simultaneously.

[0003] Traditional single-channel pipettes can only transfer one liquid sample at a time. When dealing with multi-well samples such as 96-well plates, a large number of repetitive operations are required. This not only consumes a lot of time and energy of the experimenters, but also easily leads to fatigue due to long-term operation, which in turn causes operational errors and affects the accuracy and consistency of experimental results. Even multi-channel pipettes can usually only process a limited number of samples at the same time. For the sample loading of 96-well plates, the efficiency improvement is still limited and cannot meet the growing demand for large-scale and rapid experiments.

[0004] Meanwhile, during repeated sample addition, the risk of sample contamination is greatly increased due to frequent changes in pipette tips and contact with different reagents and samples. Once the sample is contaminated, it will not only lead to inaccurate experimental data and may make the entire experimental results unreliable, but may also require the experiment to be repeated, resulting in a waste of time, reagents and samples. In view of this, this utility model is proposed. Utility Model Content

[0005] The technical problem to be solved by this utility model is to overcome the shortcomings of the prior art and provide a manual 96-well pipette that can overcome or at least partially solve the above problems.

[0006] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by this utility model is: a manual 96-well pipette, including a pipette body, and further including: a pipetting negative pressure chamber, which is threadedly connected to the aspiration end of the pipette body; 96 first negative pressure needles, which are equidistantly installed at the bottom of the pipetting negative pressure chamber, and the first negative pressure needles are connected to the inside of the pipetting negative pressure chamber.

[0007] To facilitate the adaptation of the pipette to pipettes of different inner diameters, a second negative pressure needle is further included. The second negative pressure needle is installed at the bottom of the negative pressure pipetting chamber near the first negative pressure needle and is connected to the interior of the negative pressure pipetting chamber. The size of the second negative pressure needle 203 is smaller than that of the first negative pressure needle. A sealing component for sealing the first negative pressure needle or the second negative pressure needle is provided inside the negative pressure pipetting chamber.

[0008] Furthermore, the sealing assembly includes a movable plate, 96 rubber end caps, a first magnetic plate, a second magnetic plate with a handle, a screw, and a pressure plate. The movable plate is slidably connected inside the negative pressure pipetting chamber. The 96 rubber end caps are equidistantly installed on the lower end face of the movable plate. The first magnetic plate is symmetrically embedded on both sides of the movable plate. T-shaped grooves are symmetrically opened on both sides of the negative pressure pipetting chamber. The second magnetic plate is slidably connected in the T-shaped groove. The first and second magnetic plates on the same side are magnetically attracted to each other. The screw is symmetrically connected to the negative pressure pipetting chamber by threads. The pressure plate is symmetrically slidably connected inside the negative pressure pipetting chamber. One end of the screw inside the negative pressure pipetting chamber is rotatably connected to the pressure plate on the same side.

[0009] To ensure that all pipettes can be smoothly separated from the negative pressure needle, the bottom of the negative pressure pipetting chamber is further provided with a push plate, which has a port for use with the first negative pressure needle and the second negative pressure needle. The negative pressure pipetting chamber is provided with a limiting component for limiting the push plate.

[0010] Furthermore, the limiting assembly includes a first support plate, a second support plate, a tension spring, and a guide rod. The first support plate is symmetrically fixedly connected to both sides of the push plate, and the second support plate is fixedly connected to the upper part of the negative pressure pipetting chamber near the first support plate. The tension spring is equidistantly fixedly connected between the first support plate and the second support plate, and the guide rod is fixedly connected to the first support plate and slidably connected to the second support plate.

[0011] To facilitate quick replacement of the first and second negative pressure needles and improve the sealing between the first and second negative pressure needles and the pipetting negative pressure chamber, the first and second negative pressure needles are further detachably connected to the pipetting negative pressure chamber via threads, and both the first and second negative pressure needles are equipped with sealing rings for sealing.

[0012] After adopting the above technical solution, this utility model has the following beneficial effects compared with the prior art: Compared with traditional pipettes that can only process one or a few samples at a time, this manual 96-well pipette can add 96 samples at once, which greatly shortens the overall sample addition time, speeds up the experimental process, and significantly improves experimental efficiency. Since this pipette can add the required reagents at once, it avoids the risk of contamination caused by frequent operations during repeated sample addition, ensuring the purity of experimental samples and the accuracy of experimental results. Its characteristic of processing 96 samples in one operation reduces the frequency of manual operation, allowing staff to complete more experimental tasks in the same amount of time, effectively improving overall work efficiency. At the same time, the reduction in manpower also reduces labor costs, showing obvious advantages in large-scale experiments or daily testing work.

[0013] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings. Attached Figure Description

[0014] In the attached diagram:

[0015] Figure 1 This is a schematic diagram of the structure of the present invention. Figure 1 ;

[0016] Figure 2 This is a schematic diagram of the structure of the present invention. Figure 2 ;

[0017] Figure 3 This is a schematic diagram of the internal structure of the negative pressure chamber for pipetting in this utility model;

[0018] Figure 4 This is a cross-sectional schematic diagram of a portion of the structure of this utility model;

[0019] Figure 5 This utility model Figure 4 A schematic diagram of the structure of part A.

[0020] In the diagram: 1. Pipette body; 2. Pipette negative pressure chamber; 202. First negative pressure needle; 203. Second negative pressure needle; 204. Moving plate; 205. Rubber end cap; 206. First magnetic plate; 207. Second magnetic plate; 208. Screw; 209. Pressure plate; 3. Sealing ring; 4. Push plate; 401. First support plate; 402. Second support plate; 403. Tension spring; 404. Guide rod. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate this utility model, but are not intended to limit the scope of this utility model.

[0022] Example 1:

[0023] Reference Figures 1-5 A manual 96-well pipette includes a pipette body 1 and a pipetting negative pressure chamber 2, which is threaded to the aspiration end of the pipette body 1; 96 first negative pressure needles 202 are equidistantly installed at the bottom of the pipetting negative pressure chamber 2, and the first negative pressure needles 202 are connected to the inside of the pipetting negative pressure chamber 2.

[0024] Before using this manual 96-well pipette, the negative pressure chamber 2 must be securely installed to the aspiration end of the pipette body 1 via a threaded connection. Then, holding the pipette body 1, precisely insert the 96 first negative pressure needles 202 into the 96 pipette tubes respectively, thereby establishing a communication channel between the pipette tubes and the negative pressure chamber 2.

[0025] The working principle of this pipette is based on controlling the air pressure within the negative pressure chamber 2. In operation, pressing the pipette body 1 forces the air out of the negative pressure chamber 2, creating a negative pressure environment. At this point, the 96 connected pipettes are immersed in the liquid to be transferred. Releasing the pipette body 1 allows the liquid to be forced into the pipettes by atmospheric pressure, successfully completing the aspiration step. When it is necessary to transfer the liquid to the target container, pressing the pipette body 1 again increases the air pressure within the negative pressure chamber 2, causing the liquid to be expelled from the pipettes to the target location.

[0026] Traditional pipettes can only process one or a few samples at a time, while this manual 96-well pipette can add 96 samples at once, greatly shortening the overall sample addition time, significantly accelerating the experimental process, and significantly improving experimental efficiency.

[0027] Because this pipette can add the required reagents in one go, it avoids the risk of contamination caused by frequent operations during repeated sample additions, thus ensuring the purity of experimental samples and the accuracy of experimental results.

[0028] Its ability to process 96 samples in one operation reduces the frequency of manual operations, allowing staff to complete more experimental tasks in the same amount of time, effectively improving overall work efficiency. At the same time, the reduction in manpower also lowers labor costs, making it a clear advantage in large-scale experiments or routine testing.

[0029] Example 2:

[0030] Reference Figures 1-5 The manual 96-well pipette is basically the same as in Example 1, but further includes a second negative pressure needle 203. The second negative pressure needle 203 is installed at the bottom of the negative pressure pipetting chamber 2 near the first negative pressure needle 202 and is connected to the interior of the negative pressure pipetting chamber 2. The size of the second negative pressure needle 203 is smaller than that of the first negative pressure needle 202. The negative pressure pipetting chamber 2 is provided with a sealing component for sealing the first negative pressure needle 202 or the second negative pressure needle 203.

[0031] The sealing assembly includes a movable plate 204, 96 rubber end caps 205, a first magnetic plate 206, a second magnetic plate 207 with a handle, a screw 208, and a pressure plate 209. The movable plate 204 is slidably connected inside the negative pressure pipetting chamber 2. The 96 rubber end caps 205 are equidistantly installed on the lower end face of the movable plate 204. The first magnetic plate 206 is symmetrically embedded on both sides of the movable plate 204. T-shaped grooves are symmetrically opened on both sides of the negative pressure pipetting chamber 2. The second magnetic plate 207 is slidably connected in the T-shaped groove. The first magnetic plate 206 and the second magnetic plate 207 on the same side are magnetically attracted to each other. The screw 208 is symmetrically connected to the negative pressure pipetting chamber 2 by threads. The pressure plate 209 is symmetrically slidably connected inside the negative pressure pipetting chamber 2. One end of the screw 208 inside the negative pressure pipetting chamber 2 is rotatably connected to the pressure plate 209 on the same side.

[0032] The second negative pressure needle 203 is smaller than the first negative pressure needle 202 and communicates with the inside of the negative pressure pipetting chamber 2. It is installed near the first negative pressure needle 202, allowing the pipette to be adapted to pipettes of different inner diameters. When encountering thinner pipettes, the second negative pressure needle 203 can be used, expanding the pipette's applicability. The 96 rubber caps 205 installed on the moving plate 204, in conjunction with the sliding of the moving plate 204 within the negative pressure pipetting chamber 2, can precisely position the first negative pressure needle 202. Alternatively, the second negative pressure needle 203 can be used for sealing. When different sizes of pipettes are needed, the second magnetic plate 207 with a handle is operated. The magnetic attraction between the first magnetic plate 206 and the second magnetic plate 207 drives the moving plate 204 to slide, so that the rubber end cap 205 seals the corresponding needle. This is convenient and quick. At the same time, the screw 208 and the pressure plate 209 can stabilize the moving plate 204, preventing it from shifting during the pipetting process and ensuring the accuracy and stability of the pipetting.

[0033] Example 3:

[0034] Reference Figures 1-5 The manual 96-well pipette is basically the same as in Example 2, but further: a push plate 4 is provided at the bottom of the pipetting negative pressure chamber 2, and the push plate 4 has a through-hole for use with the first negative pressure needle 202 and the second negative pressure needle 203. A limiting component for limiting the push plate 4 is provided on the pipetting negative pressure chamber 2.

[0035] The limiting assembly includes a first support plate 401, a second support plate 402, a tension spring 403, and a guide rod 404. The first support plate 401 is symmetrically fixedly connected to both sides of the push plate 4. The second support plate 402 is fixedly connected to the negative pressure chamber 2 above the first support plate 401. The tension spring 403 is fixedly connected at equal intervals between the first support plate 401 and the second support plate 402. The guide rod 404 is fixedly connected to the first support plate 401 and slidably connected to the second support plate 402.

[0036] The push plate 4 has an opening for mates with a negative pressure needle, facilitating the connection between the pipette and the negative pressure needle. When separation is required, the tension spring 403 in the limiting assembly connects the first support plate 401 and the second support plate 402. In its natural state, this allows the push plate 4 to maintain a relatively stable position. When the pipette needs to be separated, the operator can apply external force to the push plate 4 to overcome the tension of the tension spring 403 and move it downwards. At this time, the push plate 4 will apply a downward force to the pipette, thereby gently dislodging the pipette from the first negative pressure needle 202 or the second negative pressure needle 203. The sliding connection between the guide rod 404 and the second support plate 402 provides precise guidance for the movement of the push plate 4, ensuring that the push plate 4 will not deviate or shake during movement. This allows the push plate 4 to act evenly on the pipettes, ensuring that all pipettes can be smoothly separated from the negative pressure needle, avoiding incomplete separation of some pipettes. This design not only improves the convenience and efficiency of the separation operation, but also reduces the risk of damage to the pipettes or negative pressure needles due to improper manual separation, extends the service life of the pipette components, and helps to reduce experimental costs.

[0037] Example 4:

[0038] Reference Figures 1-5 The manual 96-well pipette is basically the same as in Example 3, but with the following additional features: the first negative pressure needle 202 and the second negative pressure needle 203 are detachably connected to the pipetting negative pressure chamber 2 via threads, and both the first negative pressure needle 202 and the second negative pressure needle 203 are provided with sealing rings 3 for sealing.

[0039] The first and second negative pressure needles are threadedly connected to the negative pressure pipetting chamber 2. During the experiment, the needles are easily contaminated or damaged. The experimenter can quickly replace them by manually rotating the threads without complicated tools or skills, which greatly shortens the maintenance time and ensures the continuous normal operation of the pipette. The sealing ring 3 fits tightly with the connection between the needle and the negative pressure pipetting chamber 2, effectively preventing air leakage, ensuring stable air pressure in the negative pressure pipetting chamber 2, preventing insufficient aspiration and incomplete drainage, avoiding liquid leakage and contamination, ensuring accurate pipetting, and improving the reliability of experimental results.

[0040] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model in any way. Although the present utility model has been disclosed above with reference to a preferred embodiment, it is not intended to limit the present utility model.

Claims

1. A manual 96- well pipette characterized in that, The pipette body (1) further comprises: A pipette negative pressure chamber (2) is threadedly connected to the suction end of the pipette body (1); 96 first negative pressure needle heads (202) are equidistantly arranged on the bottom of the pipette negative pressure chamber (2) and communicate with the interior of the pipette negative pressure chamber (2).

2. The manual 96-channel pipettor of claim 1, wherein, A second negative pressure needle head (203) is arranged on the bottom of the pipette negative pressure chamber (2) near the first negative pressure needle head (202) and communicates with the interior of the pipette negative pressure chamber (2), the size of the second negative pressure needle head (203) is smaller than that of the first negative pressure needle head (202), and a plugging assembly for plugging the first negative pressure needle head (202) or the second negative pressure needle head (203) is arranged in the pipette negative pressure chamber (2).

3. The manual 96-channel pipettor of claim 2, wherein, The plugging assembly comprises a moving plate (204), 96 rubber sealing heads (205), a first magnetic plate (206), a second magnetic plate (207) with a handle, a screw rod (208) and a pressing plate (209), the moving plate (204) is slidably connected in the pipette negative pressure chamber (2), the 96 rubber sealing heads (205) are equidistantly arranged on the lower end face of the moving plate (204), the first magnetic plates (206) are symmetrically embedded on the two sides of the moving plate (204), T-shaped sliding grooves are symmetrically arranged on the two sides of the pipette negative pressure chamber (2), the second magnetic plates (207) are slidably connected in the T-shaped sliding grooves, the first magnetic plates (206) and the second magnetic plates (207) on the same side are magnetically attracted to each other, the screw rod (208) is symmetrically connected to the pipette negative pressure chamber (2) through threads, the pressing plates (209) are symmetrically slidably connected in the pipette negative pressure chamber (2), and one end of the screw rod (208) in the pipette negative pressure chamber (2) is rotatably connected to the pressing plate (209) on the same side.

4. The manual 96-channel pipettor of claim 2, wherein, A push plate (4) is arranged on the bottom of the pipette negative pressure chamber (2), a through hole is arranged on the push plate (4) and used in cooperation with the first negative pressure needle head (202) and the second negative pressure needle head (203), and a limiting assembly for limiting the push plate (4) is arranged on the pipette negative pressure chamber (2).

5. The manual 96-channel pipettor of claim 4, wherein, The limiting assembly comprises first supporting plates (401), second supporting plates (402), tension springs (403) and guide rods (404), the first supporting plates (401) are fixedly connected to the two sides of the push plate (4), the second supporting plates (402) are fixedly connected to the pipette negative pressure chamber (2) above the first supporting plates (401), the tension springs (403) are equidistantly fixedly connected between the first supporting plates (401) and the second supporting plates (402), and the guide rods (404) are fixedly connected to the first supporting plates (401) and slidably connected to the second supporting plates (402).

6. The manual 96-channel pipettor of claim 2, wherein, The first negative pressure needle head (202) and the second negative pressure needle head (203) are detachably connected to the pipette negative pressure chamber (2) through threads, and a sealing ring (3) for sealing is arranged on the first negative pressure needle head (202) and the second negative pressure needle head (203).

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