Aquatic product quality safety detection device for rapidly screening food-borne pathogenic bacteria

Abstract

The invention relates to the technical field of aquatic product quality safety detection, in particular to an aquatic product quality safety detection device for rapidly screening food-borne pathogenic bacteria. Comprising a workbench, a support, a suction head bin, a sample frame, a detection module, a suction head recovery box, a movement module and a pipetting module, the support is fixedly arranged at the top of the workbench, and the suction head bin, the sample frame, the detection module and the suction head recovery box are arranged between the workbench and the support and are sequentially arranged from left to right; the movement module is fixedly arranged at the inner top of the bracket, and the pipetting module is movably arranged below the movement module; an integrated, processized and automatic detection platform is constructed, the complete process from suction head installation, sample / reagent suction, liquid transfer and distribution to final suction head discarding is automatically completed, instability and fatigability of manual operation are avoided, and the overall detection efficiency and consistency are remarkably improved.

Description

Technical Field

[0001] This invention relates to the field of aquatic product quality and safety testing technology, and in particular to an aquatic product quality and safety testing device for rapid screening of foodborne pathogens. Background Technology

[0002] Aquatic products are highly susceptible to contamination by foodborne pathogens such as Vibrio parahaemolyticus, Salmonella, and Listeria monocytogenes during aquaculture, fishing, processing, storage, and transportation. Consuming contaminated aquatic products can lead to serious foodborne illnesses, threatening public health and causing significant economic losses. Therefore, establishing an efficient and accurate screening system for pathogens in aquatic products is crucial for ensuring food safety.

[0003] Currently, the detection of pathogens in aquatic products mainly relies on traditional microbial culture methods, molecular biological detection methods, and immunological detection methods. Traditional microbial culture methods provide accurate and reliable results and are relatively inexpensive, but they are time-consuming (usually 3-7 days), involve cumbersome procedures, and require a large workload. Molecular biological detection methods, including polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), and gene chip technology, are highly specific and sensitive, and the detection time can be shortened to several hours, but the equipment is expensive and requires highly skilled operators. Immunological detection methods, including enzyme-linked immunosorbent assay (ELISA) and immunochromatographic test strips, are faster (several hours to a day) and relatively simple to operate, making them suitable for large-scale screening, but their sensitivity is lower than that of molecular biological detection methods.

[0004] Molecular biology and immunological detection methods require the transfer and addition of large quantities of samples. Currently, laboratories mainly rely on manual pipettes for liquid handling, including single-channel, multi-channel, and motorized pipettes. Although throughput and consistency are gradually improving, efficiency gains are limited, and the risk of cross-contamination cannot be avoided. Automated liquid handling workstations offer high throughput, high efficiency, and good consistency, but they are expensive to invest in and lack flexibility. For many small and medium-sized testing laboratories, customs rapid screening points, or corporate quality inspection departments, the threshold for purchasing and operating such equipment is too high.

[0005] Therefore, there is an urgent need for aquatic product quality and safety testing equipment for rapid screening of foodborne pathogens to solve the above-mentioned technical problems. Summary of the Invention

[0006] The purpose of this invention is to solve the problems in the background art and provide a device for rapid screening of foodborne pathogens in aquatic products for quality and safety testing.

[0007] The above-mentioned technical objective of the present invention is achieved through the following technical solution: A rapid screening device for foodborne pathogens in aquatic products includes a workbench, a support, a pipette tip compartment, a sample rack, a detection module, a pipette tip recovery box, a motion module, and a pipetting module. The support is fixedly mounted on the top of the workbench. The pipette tip compartment, sample rack, detection module, and pipette tip recovery box are arranged between the workbench and the support from left to right. The motion module is fixedly mounted on the inner top of the support, and the pipetting module is movable below the motion module.

[0008] Preferably, the detection module includes a detection fixture and a detection reactor, with the detection reactor mounted on the detection fixture. After the pipetting module completes the aspiration of the sample or reagent, the motion module drives it above the detection module and adds the sample or reagent into the detection reactor. The detection fixture ensures that the detection reactor does not shift during sample addition and other operations. Operators can flexibly select the most suitable detection reactor and install it on the device based on the type of pathogen to be tested, detection sensitivity, cost budget, and site conditions.

[0009] Preferably, the motion module includes a Y-axis guide rail, a Y-axis slider, an X-axis guide rail, an X-axis slider, and a Z-axis telescopic rod. The Y-axis guide rail is fixedly mounted on the inner top of the bracket and extends along the Y-axis. The Y-axis slider is slidably mounted below the Y-axis guide rail. The X-axis guide rail is fixedly mounted at the bottom of the Y-axis slider and slidably mounted below the X-axis guide rail. The motor end of the Z-axis telescopic rod is fixedly mounted at the bottom of the X-axis slider, and the telescopic end of the Z-axis telescopic rod is fixedly connected to the pipetting module. The Y-axis slider drives the X-axis guide rail to move along the Y-axis guide rail, realizing the movement of the pipetting module in the width direction of the worktable. The X-axis slider drives the Z-axis telescopic rod to move along the X-axis guide rail, realizing the movement of the pipetting module in the length direction of the worktable. The Z-axis telescopic rod drives the pipetting module to move in the vertical direction. The position of the pipetting module in three-dimensional space is precisely controlled by three independent linear motion axes, ensuring the repeatability and positioning accuracy of the pipetting operation and providing a stable and efficient basic motion platform for automated liquid handling processes.

[0010] Preferably, the pipetting module includes a top support plate, side support plates, a pitch-changing mechanism, a pressing mechanism, and multiple clamping mechanisms. The top support plate is fixedly disposed at the bottom of the telescopic end of the Z-axis telescopic rod. The two side support plates are fixedly disposed at the bottom of the top support plate. The pitch-changing mechanism and the pressing mechanism are disposed below the top support plate. The clamping mechanisms are fixedly disposed outside the pitch-changing mechanism. Multiple pipettes are securely clamped by the multiple clamping mechanisms. The pitch-changing mechanism changes the spacing between the multiple pipettes. The pressing mechanism controls the aspiration and dispensing of the multiple pipettes. The top support plate and the side support plates provide stable support for the pitch-changing mechanism and the pressing mechanism.

[0011] Preferably, the pitch-changing mechanism includes a pitch-changing screw, a pitch-changing connecting block, a pitch-changing plate, a pitch-changing fixing seat, a limiting sleeve, a pitch-changing guide rod, and a pitch-changing motor driving the pitch-changing screw. The pitch-changing motor is fixedly mounted on the top of the supporting top plate. The pitch-changing screw extends vertically from the output end of the pitch-changing motor and penetrates the supporting top plate. The pitch-changing plate is located outside the pitch-changing screw. Each of the supporting side plates has a pitch-changing guide groove extending vertically on its inner side facing each other. The two sides of the pitch-changing plate are slidably disposed in the pitch-changing guide groove. The pitch-changing connecting block is fixedly mounted on the inner side of the pitch-changing plate and sleeved on the pitch-changing screw. Multiple pitch-changing fixing seats are located outside the pitch-changing plate. Multiple stroke grooves are formed on the pitch-changing plate. The limiting sleeve is fixedly mounted on the inner side of each pitch-changing fixing seat and slidably embedded in each stroke groove. The pitch-changing guide rod is fixedly disposed between two of the supporting side plates along the waterline. The variable pitch mechanism extends horizontally and penetrates the variable pitch fixing seat. Its core working logic is to convert rotational motion into linear motion using a lead screw drive, and then simultaneously decompose the single vertical linear motion into multiple horizontal linear motions through a mechanical structure. This allows for the coordinated adjustment of the spacing between multiple clamping mechanisms. The variable pitch motor drives the variable pitch screw to rotate, and the variable pitch connecting block sleeved on the variable pitch screw rises and falls accordingly, causing the variable pitch plate to move along the variable pitch guide groove. When the variable pitch plate rises and falls, the stroke groove causes the limiting sleeve passing through it to move horizontally, causing the variable pitch fixing seat to move left and right along the variable pitch guide rod. This achieves synchronous and equidistant adjustment of each variable pitch fixing seat, enabling the pipetting module to adjust the pipette spacing according to different needs. The overall structure is compact, the transmission is precise, ensuring repeatability and consistency, and greatly improving the equipment's versatility and automation level.

[0012] Preferably, the pressing mechanism includes a first telescopic rod, a first crossbeam, a second telescopic rod, and a second crossbeam. The second telescopic rod and the second crossbeam are located outside the first telescopic rod and the first crossbeam. The motor ends of the first and second telescopic rods are fixedly mounted on the bottom of the supporting top plate. The first and second crossbeams are located above the clamping mechanism and are respectively fixedly connected to the telescopic rod ends of the first and second telescopic rods. The supporting side plates have a first pressing guide groove and a second pressing guide groove extending vertically on their inner sides facing each other. The second pressing guide groove is located outside the first pressing guide groove. The two ends of the first and second crossbeams are respectively slidably mounted on... In the first and second pressing guide grooves; when liquid needs to be drawn, the telescopic end of the first telescopic rod extends downward, and the first crossbeam moves downward along the first pressing guide groove, simultaneously contacting the piston push rods on the top of multiple pipettes and continuously pressing down to expel some air and draw in liquid using atmospheric pressure; when liquid needs to be dispensed, the telescopic end of the first telescopic rod extends downward, and the first crossbeam continues to press down simultaneously on multiple piston push rods to push air out of the liquid; when the pipette tip needs to be discarded, the telescopic end of the second telescopic rod extends downward, and the second crossbeam moves downward along the second pressing guide groove, simultaneously contacting the tip ejection buttons on multiple pipettes and continuously pressing down to push the tip out.

[0013] Preferably, each clamping mechanism includes a fixed frame, a clamping base, and movable claws. The clamping base includes a left clamping base and a right clamping base, and the movable claws include a left movable claw and a right movable claw. The fixed frame is fixedly disposed on the outside of the variable pitch fixing seat. Straight grooves extending horizontally are formed on the upper and lower inner walls of the fixed frame. The upper and lower ends of the left and right clamping bases are slidably disposed in the straight grooves. The left and right movable claws are respectively fixedly disposed on the outside of the left and right clamping bases. When the left and right clamping bases move away from each other along the straight grooves, the left and right movable claws open outwards, allowing the operator to place or remove the pipette. When the left and right clamping bases move towards each other along the straight grooves, the left and right movable claws close inwards, and the pipette is securely clamped by the movable claws.

[0014] Preferably, each clamping mechanism further includes a clamping connecting rod, a control cam, a positioning block, and a clamping motor that drives the control cam. The positioning block includes a left positioning block and a right positioning block. The clamping motor is fixedly mounted at the end of the limiting sleeve. The clamping connecting rod is located at the output end of the clamping motor and passes through the limiting sleeve and the fixing frame. The control cam is located between the clamping connecting rod and the clamping base and is fixedly connected to the clamping connecting rod. A left helical groove and a right helical groove are formed on the outer surface of the control cam. The left positioning block and the right positioning block are located on the inner sides of the left clamping base and the right clamping base, respectively, and are slidably embedded therein. In the left and right helical grooves; the clamping motor drives the clamping connecting rod to rotate, the control cam rotates accordingly, and the left and right positioning blocks slide along the left and right helical grooves. The left and right helical grooves are symmetrical about the rotation axis of the control cam, so they can drive the left and right positioning blocks to move in opposite directions or in opposite directions in the horizontal direction, thereby driving the left and right clamping bases to move in opposite directions or in opposite directions along the straight groove, realizing the opening and closing of the movable jaws. The cam mechanism has direct transmission, fast response, and compact structure, ensuring the synchronicity of the movement of the left and right movable jaws.

[0015] Preferably, elastic anti-slip pads are fixedly provided on the inner surfaces of both the left and right movable jaws; during the clamping process of the pipette, the elastic anti-slip pads are compressed and deformed, filling the microscopic gap between the movable jaws and the outer surface of the pipette, increasing the actual contact area, and providing greater friction, significantly improving clamping reliability, and effectively preventing the pipette from shaking, sliding or even falling off when it is in motion or subjected to vibration.

[0016] Preferably, the suction head recycling box includes a box body, a cover plate, a mounting base, an opening and closing mechanism, and a recycling motor that drives the opening and closing mechanism. The box body is disposed on the workbench, the cover plate is rotatably disposed on the top of the box body, the mounting base is fixedly disposed on the bottom surface of the cover plate, and the recycling motor is fixedly disposed on the outside of the box body. The opening and closing mechanism includes an active connecting rod, an active moving rod, a first driven rod, a second driven rod, and an opening and closing connecting rod. The active connecting rod is disposed at the output end of the recycling motor and passes through the box body. The opening and closing connecting rod is disposed inside the box body. One end of the active moving rod is fixedly connected to the active connecting rod, and the other end of the active moving rod is rotatably disposed on the opening and closing connecting rod. One end of the first driven rod and the second driven rod... The first and second driven rods are rotatably mounted on the mounting base, and the other ends of both are rotatably mounted on the opening and closing connecting rod. The recycling motor drives the active connecting rod to rotate, and the active moving rod rotates accordingly. The movable end of the active moving rod is hinged to the opening and closing connecting rod, thereby driving the opening and closing connecting rod to move in the vertical plane. The first and second driven rods, hinged to the opening and closing connecting rod, move accordingly, and the mounting base, which is hinged to the other ends of the first and second driven rods, moves accordingly, thereby driving the cover plate to rotate around the hinge point between itself and the box body, realizing the opening and closing of the cover plate, avoiding the inconvenience of manual opening and closing, and preventing aerosol pollution or odor emission caused by the opening of the box body.

[0017] In summary, the present invention has the following beneficial effects: This invention integrates pipette tip supply, sample / reagent storage, reaction / detection, and waste recycling into a streamlined process on the workbench. Combined with the motion module and pipetting module, it constructs an integrated, streamlined, and automated detection platform. This platform automatically completes the entire process from pipette tip installation, sample / reagent aspiration, liquid transfer and dispensing to final pipette tip disposal, avoiding the instability and fatigue associated with manual operation and significantly improving the overall efficiency and consistency of detection.

[0018] The motion module of this invention achieves high-precision three-dimensional spatial positioning, providing a stable and repeatable spatial positioning foundation for automated processes. The clamping mechanism ensures stable clamping of the pipette, guaranteeing the stability of the pipetting operation. Multiple pipettes increase the detection throughput. The variable-pitch mechanism enables synchronous adjustment of the spacing between multiple channels, adapting to different spacing arrangements. The pressing mechanism provides synchronous downward pressure for all pipettes, ensuring the consistency of the pipetting volume.

[0019] This invention enables the automatic opening and closing of the suction head recycling box by setting the opening and closing mechanism, avoiding direct contact between laboratory personnel and contaminated waste, improving operational safety, further shortening the process cycle, and improving overall operating efficiency. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the pipetting module structure of the present invention; Figure 3 This is a schematic diagram of the variable pitch guide groove, the first pressing guide groove, and the second pressing guide groove of the present invention; Figure 4 This is a schematic diagram of the variable pitch mechanism structure of the present invention; Figure 5 This is a schematic diagram of the variable pitch mechanism structure of the present invention; Figure 6 This is a schematic diagram of the clamping mechanism structure of the present invention; Figure 7 This is a schematic diagram of the fixed frame structure of the present invention; Figure 8 This is the present invention. Figure 6 An enlarged view of point A; Figure 9 This is a schematic diagram of the suction head recycling box structure of the present invention; Figure 10 This is a schematic diagram of the suction head recycling box structure of the present invention; Figure 11 This is a schematic cross-sectional view of the pipette structure of the present invention; Figure 12 This is the present invention. Figure 11 An enlarged view of point B; In the diagram, 1. Workbench; 11. Support; 12. Pipe tip compartment; 13. Sample rack; 14. Detection module; 15. Detection fixture; 16. Detection reactor; 2. Motion module; 21. Y-axis guide rail; 22. Y-axis slider; 23. X-axis guide rail; 24. X-axis slider; 25. Z-axis telescopic rod; 3. Pipetting module; 31. Support top plate; 32. Support side plate; 33. Variable pitch guide groove; 34. First pressing guide groove; 5. Second pressing guide groove; 4. Pitch-changing mechanism; 41. Pitch-changing screw; 42. Pitch-changing connecting block; 43. Pitch-changing plate; 44. Pitch-changing fixing seat; 45. Limiting sleeve; 46. Pitch-changing guide rod; 47. Pitch-changing motor; 48. Stroke groove; 5. Pressing mechanism; 51. First telescopic rod; 52. First crossbeam; 53. Second telescopic rod; 54. Second crossbeam; 6. Clamping mechanism; 61. Fixed frame; 611. Straight groove; 62. 621. Clamping base; 622. Left clamping base; 623. Right clamping base; 64. Movable jaw; 65. Left movable jaw; 66. Right movable jaw; 67. Clamping connecting rod; 68. Control cam; 69. Left helical groove; 60. Right helical groove; 61. Positioning block; 62. Left positioning block; 63. Right positioning block; 64. Clamping motor; 75. Elastic anti-slip pad; 8. Suction head recycling box; 76. Box body; 77. 73. Cover plate; 74. Mounting base; 8. Recycling motor; 9. Opening and closing mechanism; 10. Active connecting rod; 11. Active moving rod; 12. First driven rod; 13. Second driven rod; 14. Opening and closing connecting rod; 15. Pipette; 16. Pipette body; 17. Sleeve; 18. Push rod; 19. Piston; 10. Tip; 11. Tip ejection sleeve; 12. Tip ejection button; 13. Limit block; 14. Limit groove; 15. Tip ejection spring. Detailed Implementation

[0021] The present invention will be further described in detail below with reference to the accompanying drawings.

[0022] Example

[0023] according to Figure 1 As shown, the aquatic product quality and safety testing device for rapid screening of foodborne pathogens includes a workbench 1, a support 11, a pipette tip chamber 12, a sample rack 13, a detection module 14, a pipette tip recovery box 7, a motion module 2, and a pipetting module 3. The support 11 is fixedly installed on the top of the workbench 1. The pipette tip chamber 12, the sample rack 13, the detection module 14, and the pipette tip recovery box 7 are arranged between the workbench 1 and the support 11 from left to right. The motion module 2 is fixedly installed on the inner top of the support 11, and the pipetting module 3 is movable below the motion module 2.

[0024] according to Figure 1As shown, the detection module 14 includes a detection fixture 15 and a detection reactor 16, with the detection reactor 16 mounted on the detection fixture 15. The detection reactor 16 includes, but is not limited to, real-time fluorescence PCR kits, isothermal amplification detection kits, immunochromatographic test strips, microfluidic chips, etc.

[0025] according to Figure 1 As shown, the motion module 2 includes a Y-axis guide rail 21, a Y-axis slider 22, an X-axis guide rail 23, an X-axis slider 24, and a Z-axis telescopic rod 25. The Y-axis guide rail 21 is fixedly installed on the inner top of the bracket 11 and extends along the Y-axis. The Y-axis slider 22 is slidably installed below the Y-axis guide rail 21. The X-axis guide rail 23 is fixedly installed at the bottom of the Y-axis slider 22. The X-axis slider 24 is slidably installed below the X-axis guide rail 23. The motor end of the Z-axis telescopic rod 25 is fixedly installed at the bottom of the X-axis slider 24. The telescopic end of the Z-axis telescopic rod 25 is fixedly connected to the pipetting module 3.

[0026] according to Figure 2 As shown, the pipetting module 3 includes a top support plate 31, two side support plates 32, a pitch mechanism 4, a pressing mechanism 5, and multiple clamping mechanisms 6. The top support plate 31 is fixedly mounted on the bottom of the telescopic end of the Z-axis telescopic rod 25. The two side support plates 32 are fixedly mounted on the bottom of the top support plate 31. The pitch mechanism 4 and the pressing mechanism 5 are located below the top support plate 31. The clamping mechanisms 6 are fixedly mounted on the outside of the pitch mechanism 4. When aspirating or dispensing liquid, the pressing mechanism 5 presses down on the piston rod of the pipette mounted on the clamping mechanism 6 to generate negative pressure to aspirate or positive pressure to dispense liquid.

[0027] according to Figures 3-5 As shown, the pitch mechanism 4 includes a pitch screw 41, a pitch connecting block 42, a pitch plate 43, a pitch fixing seat 44, a limiting sleeve 45, a pitch guide rod 46, and a pitch motor 47 that drives the pitch screw 41. The pitch motor 47 is fixedly mounted on the top of the supporting top plate 31. The pitch screw 41 is located at the output end of the pitch motor 47, extends vertically, and passes through the supporting top plate 31. The pitch plate 43 is located on the outside of the pitch screw 41. Pitch guide grooves 33 extending vertically are opened on the inner sides of the supporting side plates 32 facing each other. The two sides of the pitch plate 43 are slidably mounted in the pitch guide grooves 33. The pitch connecting block 42 is fixedly mounted on the top of the supporting top plate 31. The pitch plate 43 is sleeved on the inner side of the pitch screw 41, and multiple pitch fixing seats 44 are provided on the outer side of the pitch plate 43. Multiple stroke grooves 48 are provided on the pitch plate 43. The limiting sleeve 45 is fixedly provided on the inner side of each pitch fixing seat 44 and slidably embedded in each stroke groove 48. The pitch guide rod 46 is fixedly provided between the two support side plates 32, extends horizontally and passes through the pitch fixing seat 44. The pitch guide groove 33 ensures the accuracy of the movement of the pitch plate 43 in the vertical direction and avoids the shaking caused by the screw drive. The pitch guide rod 46 provides a horizontal movement guide for the pitch fixing seat 44 to ensure the straightness and stability of the horizontal movement.

[0028] according to Figure 2 As shown, the pressing mechanism 5 includes a first telescopic rod 51, a first crossbeam 52, a second telescopic rod 53, and a second crossbeam 54. The second telescopic rod 53 and the second crossbeam 54 are located outside the first telescopic rod 51 and the first crossbeam 52, respectively. The motor ends of the first telescopic rod 51 and the second telescopic rod 53 are both fixedly located at the bottom of the supporting top plate 31. The first crossbeam 52 and the second crossbeam 54 are both located above the clamping mechanism 6 and are fixedly connected to the telescopic rod ends of the first telescopic rod 51 and the second telescopic rod 53, respectively. The inner sides of the supporting side plates 32 facing each other are provided with a first pressing guide groove 34 and a second pressing guide groove 35 extending vertically. The second pressing guide groove 35 is located outside the first pressing guide groove 34. The two ends of the first crossbeam 52 and the second crossbeam 54 are slidably located in the first pressing guide groove 34 and the second pressing guide groove 35, respectively. The first pressing guide groove 34 and the second pressing guide groove 35 provide vertical movement guides for the first crossbeam 52 and the second crossbeam 54, respectively, to prevent deviation and shaking.

[0029] according to Figure 6 , Figure 7 As shown, each clamping mechanism 6 includes a fixed frame 61, a clamping base 62, and a movable claw 63. The clamping base 62 includes a left clamping base 621 and a right clamping base 622, and the movable claw 63 includes a left movable claw 631 and a right movable claw 632. The fixed frame 61 is fixedly mounted on the outside of the variable pitch fixing seat 44. Straight grooves 611 extending horizontally are opened on the upper and lower inner walls of the fixed frame 61. The upper and lower ends of the left clamping base 621 and the right clamping base 622 are slidably mounted in the straight grooves 611. The left movable claw 631 and the right movable claw 632 are respectively fixedly mounted on the outside of the left clamping base 621 and the right clamping base 622. The opposing clamping method can apply force evenly from both sides to ensure that the pipette is clamped vertically and to ensure the alignment of the pipette with the sample tube and the detection reactor 16.

[0030] according to Figure 6 , Figure 8As shown, each clamping mechanism 6 also includes a clamping connecting rod 64, a control cam 65, a positioning block 66, and a clamping motor 67 that drives the control cam 65. The positioning block 66 includes a left positioning block 661 and a right positioning block 662. The clamping motor 67 is fixedly mounted on the end of the limiting sleeve 45. The clamping connecting rod 64 is located at the output end of the clamping motor 67 and passes through the limiting sleeve 45 and the fixed frame 61. The control cam 65 is located between the clamping connecting rod 64 and the clamping base 62 and is fixedly connected to the clamping connecting rod 64. A left helical groove 651 and a right helical groove 652 are provided on the outer surface of 65. The left positioning block 661 and the right positioning block 662 are located on the inner side of the left clamping base 621 and the right clamping base 622, respectively, and are slidably embedded in the left helical groove 651 and the right helical groove 652. The cooperation between the left positioning block 661 and the right positioning block 662 and the left helical groove 651 and the right helical groove 652 converts the rotational motion of the control cam 65 into the linear motion of the left movable jaw 631 and the right movable jaw 632, thereby realizing automated clamping.

[0031] according to Figure 7 As shown, elastic anti-slip pads 68 are fixedly provided on the inner surfaces of the left movable jaw 631 and the right movable jaw 632; the deformation capability of the elastic anti-slip pads 68 can better adapt to pipettes of different brands and appearances, and improve the versatility of the clamping mechanism 6.

[0032] according to Figure 9 , Figure 10 As shown, the suction head recycling box 7 includes a box body 71, a cover plate 72, a mounting base 73, an opening and closing mechanism 8, and a recycling motor 74 that drives the opening and closing mechanism 8. The box body 71 is mounted on the workbench 1, the cover plate 72 is rotatably mounted on the top of the box body 71, the mounting base 73 is fixedly mounted on the bottom surface of the cover plate 72, and the recycling motor 74 is fixedly mounted on the outside of the box body 71. The opening and closing mechanism 8 includes an active connecting rod 81, an active moving rod 82, a first driven rod 83, a second driven rod 84, and an opening and closing connecting rod 85. The active connecting rod 81 is mounted on the outside of the recycling motor 74. The output end of the 4 extends through the housing 71. The opening and closing connecting rod 85 is located inside the housing 71. One end of the active motion rod 82 is fixedly connected to the active connecting rod 81, and the other end of the active motion rod 82 is rotatably mounted on the opening and closing connecting rod 85. One end of the first driven rod 83 and the second driven rod 84 are rotatably mounted on the mounting base 73, and the other ends of the first driven rod 83 and the second driven rod 84 are rotatably mounted on the opening and closing connecting rod 85. The automatic opening and closing function and the automated pipetting process are integrated, further improving the automation level of the entire device.

[0033] according to Figure 11 , Figure 12As shown, the pipette 9 includes a body 91, a sleeve 92, a push rod 93, a piston 94, a pipette tip 95, a tip ejection sleeve 96, a tip ejection button 97, and a tip ejection spring 99. The sleeve 92 is fixedly disposed at the bottom of the body 91. The push rod 93 extends vertically through the body 91. The piston 94 is fixedly disposed at the bottom of the push rod 93 and slidably disposed inside the body 91. The tip ejection sleeve 96 is sleeved on the body 91 and the sleeve 92. The tip ejection button 97 is fixedly disposed at the top of the tip ejection sleeve 96 and extends outward. A limiting groove 98 extending vertically is provided on the outer wall of 91. A limiting block 971 extending inward into the limiting groove 98 is provided on the inner side of the suction head retraction button 97. The suction head retraction spring 99 is provided in the limiting groove 98. The upper end face of the suction head retraction spring 99 is fixedly connected to the bottom face of the limiting block 971, and the lower end face of the suction head retraction spring 99 is fixedly connected to the inner bottom face of the limiting groove 98. The suction head 95 is installed at the bottom of the sleeve 92 during use. The top of the suction head 95 abuts against the bottom of the suction head retraction sleeve 96. The suction head 95 is in storage When placing the pipette tip 95 in the tip compartment 12, the tip 95 is made of a flexible but rigid plastic, mostly polypropylene, which can firmly adhere to the outer wall of the tip retraction sleeve 96 during use. The outer wall of the tip retraction sleeve 96 can be provided with anti-slip texture to increase friction. Before drawing liquid, press the push rod 93 to drive the piston 94 down, forming an air column in the sleeve 92, partially inserting the tip 95 mounted on the pipette 9 into the liquid. Release the push rod 93 to raise the piston 94, and the air column rises accordingly, thus drawing liquid into the liquid. A negative pressure is generated below the air column, i.e., in the pipette tip 95, drawing the liquid into the pipette tip 95. When transferring the liquid, press the push rod 93 to make the piston 94 descend again, and the air column descends accordingly, squeezing the liquid out of the pipette tip 95. To prevent cross-contamination, avoid interaction, and ensure pipetting accuracy, the pipette tip 95 should be replaced after one use. Press the pipette tip ejection button 97 to make the pipette tip ejection sleeve 96 descend. The bottom of the pipette tip ejection sleeve 96 pushes the pipette tip 95 installed at the bottom of the sleeve 92 until the pipette tip 95 is dislodged from the bottom of the sleeve 92.

[0034] Working principle: According to Figures 1-12As shown, the clamping motor 67 drives the left movable jaw 631 and the right movable jaw 632 to open outwards, placing the pipette body 91 of the pipette 9 between them. The clamping motor 67 then restarts, causing the left movable jaw 631 and the right movable jaw 632 to close inwards, completing the stable clamping of the pipette body 91. The motion module 2 drives the pipetting module 3 to move, moving the pipette 9 above the tip compartment 12. The variable pitch motor 47 drives the variable pitch fixing seat 44 to move, adjusting the spacing between each pipette 9 to fit the tip compartment 12. After the spacing of the pipette tips 95 is adjusted, the motion module 2 lowers the pipette 9 until the tips 95 are installed at the bottom of the pipette body 91, completing the installation of the tips 95. The motion module 2 then moves the pipette 9 above the sample holder 13. The first telescopic rod 51 pushes the first crossbeam 52 downward, simultaneously contacting the push rods 93 on the top of multiple pipettes 9 and continuously pressing down to expel some air. After the variable pitch motor 47 adjusts the spacing between each pipette 9 according to the spacing of the sample tubes, the motion module 2 moves the pipette... The first telescopic rod 51 lowers the pipette tip 95 until it partially extends into the sample liquid surface. The first telescopic rod 51 then pulls the first crossbeam 52 upwards away from the push rod 93, using atmospheric pressure to draw the sample liquid into the pipette tip 95, completing sample aspiration. The motion module 2 moves the pipette 9 above the detection module 14. The variable pitch motor 47 adjusts the spacing between the pipettes 9 according to the spacing of the detection reactor 16. The motion module 2 then lowers the pipette 9 until the bottom of the pipette tip 95 is close to the sample application point of the detection reactor 16. The first telescopic rod 51 then pushes the first crossbeam 52 upwards away from the push rod 93. The crossbeam 52 presses down on the push rod 93 at the top of the pipette 9 to expel the liquid from the pipette tip 95, completing the sample distribution. The motion module 2 moves the pipette 9 above the pipette tip collection box 7, and the collection motor 74 drives the cover plate 72 to open. The second telescopic rod 53 pushes the second crossbeam 54 to press down on the pipette tip ejection button 97 on the outside of the pipette 9 until the ejection sleeve 96 pushes the pipette tip 95 away from the sleeve 92. After discarding the used pipette tip 95, the collection motor 74 drives the cover plate 72 to close, completing the collection of the pipette tip 95.

[0035] This specific embodiment is merely an explanation of the present invention and is not intended to limit the invention. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they are within the scope of the claims of the present invention.

Claims

1. A rapid screening device for foodborne pathogens in aquatic products, comprising a workbench (1), a support (11), a pipette tip chamber (12), a sample rack (13), a detection module (14), a pipette tip recovery box (7), a motion module (2), and a pipetting module (3). The support (11) is fixedly installed on the top of the workbench (1). The pipette tip chamber (12), the sample rack (13), the detection module (14), and the pipette tip recovery box (7) are arranged between the workbench (1) and the support (11) and arranged sequentially from left to right. The motion module (2) is fixedly installed on the inner top of the support (11). The pipetting module (3) is movable below the motion module (2).

2. The aquatic product quality and safety testing device for rapid screening of foodborne pathogens according to claim 1, characterized in that, The detection module (14) includes a detection fixture (15) and a detection reactor (16), wherein the detection reactor (16) is disposed on the detection fixture (15).

3. The aquatic product quality and safety testing device for rapid screening of foodborne pathogens according to claim 1, characterized in that, The motion module (2) includes a Y-axis guide rail (21), a Y-axis slider (22), an X-axis guide rail (23), an X-axis slider (24), and a Z-axis telescopic rod (25). The Y-axis guide rail (21) is fixedly installed on the inner top of the bracket (11) and extends along the Y-axis. The Y-axis slider (22) is slidably installed below the Y-axis guide rail (21). The X-axis guide rail (23) is fixedly installed at the bottom of the Y-axis slider (22). The X-axis slider (24) is slidably installed below the X-axis guide rail (23). The motor end of the Z-axis telescopic rod (25) is fixedly installed at the bottom of the X-axis slider (24). The telescopic end of the Z-axis telescopic rod (25) is fixedly connected to the pipetting module (3).

4. The aquatic product quality and safety testing device for rapid screening of foodborne pathogens according to claim 3, characterized in that, The pipetting module (3) includes a top support plate (31), a side support plate (32), a pitch mechanism (4), a pressing mechanism (5), and multiple clamping mechanisms (6). The top support plate (31) is fixedly installed at the bottom of the telescopic end of the Z-axis telescopic rod (25). The two side support plates (32) are fixedly installed at the bottom of the top support plate (31). The pitch mechanism (4) and the pressing mechanism (5) are located below the top support plate (31). The clamping mechanisms (6) are fixedly installed on the outside of the pitch mechanism (4).

5. The aquatic product quality and safety testing device for rapid screening of foodborne pathogens according to claim 4, characterized in that, The pitch-changing mechanism (4) includes a pitch-changing screw (41), a pitch-changing connecting block (42), a pitch-changing plate (43), a pitch-changing fixing seat (44), a limiting sleeve (45), a pitch-changing guide rod (46), and a pitch-changing motor (47) that drives the pitch-changing screw (41). The pitch-changing motor (47) is fixedly mounted on the top of the supporting top plate (31). The pitch-changing screw (41) is located at the output end of the pitch-changing motor (47), extends vertically, and passes through the supporting top plate (31). The pitch-changing plate (43) is located on the outside of the pitch-changing screw (41). The supporting side plates (32) are provided with pitch-changing guide rods extending vertically on their inner sides facing each other. The variable pitch plate (43) is slidably disposed on both sides in the variable pitch guide groove (33). The variable pitch connecting block (42) is fixedly disposed on the inner side of the variable pitch plate (43) and sleeved on the variable pitch screw (41). Multiple variable pitch fixing seats (44) are disposed on the outer side of the variable pitch plate (43). Multiple stroke grooves (48) are opened on the variable pitch plate (43). The limiting sleeve (45) is fixedly disposed on the inner side of each variable pitch fixing seat (44) and slidably embedded in each stroke groove (48). The variable pitch guide rod (46) is fixedly disposed between the two support side plates (32), extends horizontally and passes through the variable pitch fixing seat (44).

6. The aquatic product quality and safety testing device for rapid screening of foodborne pathogens according to claim 4, characterized in that, The pressing mechanism (5) includes a first telescopic rod (51), a first crossbeam (52), a second telescopic rod (53), and a second crossbeam (54). The second telescopic rod (53) and the second crossbeam (54) are located outside the first telescopic rod (51) and the first crossbeam (52). The motor ends of the first telescopic rod (51) and the second telescopic rod (53) are both fixed to the bottom of the supporting top plate (31). The first crossbeam (52) and the second crossbeam (54) are both located above the clamping mechanism (6) and The telescopic rod ends of the first telescopic rod (51) and the second telescopic rod (53) are fixedly connected respectively. The support side plate (32) has a first pressing guide groove (34) and a second pressing guide groove (35) extending vertically on its inner side facing each other. The second pressing guide groove (35) is located outside the first pressing guide groove (34). The two ends of the first crossbeam (52) and the second crossbeam (54) are slidably located in the first pressing guide groove (34) and the second pressing guide groove (35) respectively.

7. The aquatic product quality and safety testing device for rapid screening of foodborne pathogens according to claim 4, characterized in that, Each clamping mechanism (6) includes a fixed frame (61), a clamping base (62), and a movable claw (63). The clamping base (62) includes a left clamping base (621) and a right clamping base (622). The movable claw (63) includes a left movable claw (631) and a right movable claw (632). The fixed frame (61) is fixedly disposed on the outside of the variable pitch fixing seat (44). The upper and lower inner walls of the fixed frame (61) are provided with straight grooves (611) extending in the horizontal direction. The upper and lower ends of the left clamping base (621) and the right clamping base (622) are slidably disposed in the straight grooves (611). The left movable claw (631) and the right movable claw (632) are respectively fixedly disposed on the outside of the left clamping base (621) and the right clamping base (622).

8. The aquatic product quality and safety testing device for rapid screening of foodborne pathogens according to claim 7, characterized in that, Each of the clamping mechanisms (6) further includes a clamping connecting rod (64), a control cam (65), a positioning block (66), and a clamping motor (67) that drives the control cam (65). The positioning block (66) includes a left positioning block (661) and a right positioning block (662). The clamping motor (67) is fixedly mounted at the end of the limiting sleeve (45). The clamping connecting rod (64) is located at the output end of the clamping motor (67) and passes through the limiting sleeve (45) and the fixing frame (61). The control cam (65) is located between the clamping connecting rod (64) and the clamping base (62) and is fixedly connected to the clamping connecting rod (64). A left helical groove (651) and a right helical groove (652) are provided on the outer side of the control cam (65). The left positioning block (661) and the right positioning block (662) are located on the inner side of the left clamping base (621) and the right clamping base (622) respectively and are slidably embedded in the left helical groove (651) and the right helical groove (652) respectively.

9. The aquatic product quality and safety testing device for rapid screening of foodborne pathogens according to claim 7, characterized in that, Elastic anti-slip pads (68) are fixedly provided on the inner sides of both the left movable claw (631) and the right movable claw (632).

10. The aquatic product quality and safety testing device for rapid screening of foodborne pathogens according to claim 1, characterized in that, The suction head recycling box (7) includes a box body (71), a cover plate (72), a mounting base (73), an opening and closing mechanism (8), and a recycling motor (74) that drives the opening and closing mechanism (8). The box body (71) is mounted on the workbench (1). The cover plate (72) is rotatably mounted on the top of the box body (71). The mounting base (73) is fixedly mounted on the bottom surface of the cover plate (72). The recycling motor (74) is fixedly mounted on the outside of the box body (71). The opening and closing mechanism (8) includes an active connecting rod (81), an active moving rod (82), a first driven rod (83), a second driven rod (84), and an opening and closing connection. The active connecting rod (81) is located at the output end of the recycling motor (74) and passes through the box (71). The opening and closing connecting rod (85) is located inside the box (71). One end of the active moving rod (82) is fixedly connected to the active connecting rod (81). The other end of the active moving rod (82) is rotatably located on the opening and closing connecting rod (85). One end of the first driven rod (83) and the second driven rod (84) are respectively rotatably located on the mounting base (73). The other ends of the first driven rod (83) and the second driven rod (84) are both rotatably located on the opening and closing connecting rod (85).

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