Magnetic linkage non-contact drive closure cassette

Abstract

The application discloses a magnetic linkage non-contact driving closed cartridge, relates to the technical field of closed sample processing and detection, and comprises a base and an upper cover. A pipette is arranged in the inner cavity of the upper cover. The central hole of the pipette is slidably sleeved on the cylindrical inner recess of the upper cover. The upper end surface of the pipette is provided with a support part around the central hole. The support part is provided with groove cavities. A magnetic part one is fixedly embedded in each groove cavity. A pipette tip is fixedly arranged on the outer ring of the pipette. The rotation and axial movement of the pipette are controlled by using magnetic force, thereby forming non-contact driving. The sealing property of the cartridge is effectively ensured. Meanwhile, the sealing ring and other mechanisms are avoided, the difficulty of sealing the internal space is greatly reduced, the leakage risk is reduced, the size of the driving mechanism is reduced, and the precision of pipette movement control is greatly improved.

Description

Technical Field

[0001] This invention relates to the field of sealed sample processing and detection technology, specifically a magnetically linked non-contact driven sealed cartridge. Background Technology

[0002] Sample analysis in the fields of biology, chemistry, and medicine typically involves sample preparation, reaction, separation, and detection. Most routine experimental operations are conducted in open environments, allowing samples to directly contact the outside world through air. However, some samples pose significant risks to operators, are easily affected by external interference, or cause interference to the environment, such as biological samples containing pathogenic agents. To reduce biohazards, there is an urgent need to develop a closed-loop device for sample processing, reaction, and product detection.

[0003] Achieving horizontal and vertical movement of a pipette within a closed container requires a relatively complex structure; traditional solutions typically use mechanical connections, which have the following problems:

[0004] 1. The sealing of the cartridge usually requires the use of sealing rings, such as O-rings, which poses a certain risk of leakage. This could result in the tested sample containing pathogens that are harmful to operators and the environment; it could lead to operator infection; leakage of the tested sample or reaction product could lead to contamination of the environment or operating instruments, and result in false positives in the test results of other samples.

[0005] 2. To achieve precise movement control of the pipette, a complex connection structure is usually required. As can be seen from the patent application number 201080031828.3, entitled "Device for Amplicon Rescue Multiplex PCR", a relatively complex mechanical structure such as a cam rod and lead screw is used to achieve the horizontal and vertical movement of the pipette. If the precision of the movement control is not achieved, it will lead to insufficient aspiration, excessive liquid hanging at the tip, excessive residual liquid, and in more serious cases, it may cause unexpected collisions between the pipette and other parts of the cartridge, resulting in complete failure of the pipetting function. Summary of the Invention

[0006] The purpose of this invention is to provide a magnetically linked non-contact driven enclosed card box to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a magnetically linked non-contact driven closed card box, comprising a base and a top cover, wherein the top cover has an inner cavity, and a pipette is disposed in the inner cavity. The central hole of the pipette is slidably fitted onto the cylindrical concave portion of the top cover. A support portion is disposed on the upper end face of the pipette around the central hole. Four slots are evenly distributed on the support portion, and a magnetic component is fixedly embedded in each slot. A pipette tip is fixedly disposed on the outer ring of the pipette. An inner cavity is disposed at the center of the top cover, and the inner cavity is used for the magnetic end of the driving mechanism to extend into. A limiting component for limiting the vertical movement of the pipette is disposed between the side of the pipette away from the pipette tip and the side wall of the inner cavity of the top cover.

[0008] Preferably, the top of the cover is provided with an opening, and a flexible tube is connected between the opening and the pipette tip.

[0009] Preferably, the base is disposed at the bottom of the upper cover, and a sealed space is formed between the base and the upper cover. The base and the upper cover can be detachably connected or integrated.

[0010] Preferably, the magnetic end of the driving mechanism includes a driving rod, and a disk portion is fixedly provided at the bottom end of the driving rod. Magnetic elements two are fixedly distributed around the bottom of the disk portion around the driving rod, and each magnetic element two corresponds to a magnetic element one.

[0011] Preferably, the magnetic component is a permanent magnet.

[0012] Preferably, the end of the first magnetic component near the center line of the base is the "N" pole, and the outer end of the second magnetic component is the "S" pole. The interaction force between the first magnetic component and the second magnetic component is not less than 100 mN·m in the rotational direction and not less than 5.0 N in the vertical direction.

[0013] Preferably, a limiting base is fixedly installed at the bottom of the cylindrical concave portion of the upper cover.

[0014] Preferably, the edge of the bracket portion near the central hole is rounded.

[0015] In summary, the beneficial effects of this invention are:

[0016] 1. This invention utilizes magnetic force to control the rotation and axial movement of the pipette fitting, thereby forming a non-contact drive that effectively ensures the sealing of the cartridge. At the same time, it avoids the use of sealing rings and other mechanisms, greatly reducing the difficulty of sealing the internal space and reducing the risk of leakage.

[0017] 2. The friction is also greatly reduced compared to the friction caused by the sealing ring of traditional pipettes, which helps to reduce the size of the drive mechanism. At the same time, the accuracy of pipette motion control is greatly improved, effectively avoiding insufficient liquid aspiration, large liquid hanging at the tip, excessive residual liquid, and even more serious problems such as unexpected collisions between the pipette and other parts of the cartridge, which may cause the pipetting function to fail completely. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of the invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the overall structure of a magnetically linked non-contact drive enclosed card box according to the present invention;

[0020] Figure 2 This is a schematic diagram of the main cross-sectional structure of a magnetically linked non-contact drive enclosed card box according to the present invention.

[0021] Figure 3 This is a schematic diagram of the pipetting fitting structure of a non-contact driven closed cartridge with magnetic linkage according to the present invention;

[0022] Figure 4 This is a schematic diagram of the base structure of a component in a magnetically linked non-contact drive enclosed card box according to the present invention;

[0023] Figure 5 This is a schematic diagram of the magnetic pole distribution in a non-contact drive enclosed card box with magnetic linkage according to the present invention;

[0024] Figure 6 This is a schematic diagram of the upper cover structure of a magnetically linked non-contact driven closed card box according to the present invention;

[0025] Figure 7 This is a schematic diagram of a limiting chassis structure in a magnetically linked non-contact drive enclosed card box according to the present invention.

[0026] Figure 8 This is a schematic diagram of the structure of a magnetically linked non-contact drive closed card box according to Embodiment 2 of the present invention.

[0027] The markings in the attached diagram are described as follows: base 1; pipette fitting 2; top cover 3; drive rod 4; flexible hose 5; drive ring 6; limiting base 7; support part 201; cavity 202; pipette tip 203; magnetic component one 204; concave cavity 301; opening part 302; inner cavity of top cover 303; disc part 401; magnetic component two 402. Detailed Implementation

[0028] All features disclosed in this specification, or all steps in all disclosed methods or processes, may be combined in any way, except for mutually exclusive features and / or steps.

[0029] Any feature disclosed in this specification (including any appended claims, abstract, and drawings) may be replaced by other equivalent or similar features, unless specifically stated otherwise. That is, unless specifically stated otherwise, each feature is merely one example of a series of equivalent or similar features.

[0030] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, a direct connection, or an indirect connection through an intermediate medium; they can refer to the internal communication of at least two elements or the interaction relationship of at least two elements, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0031] The following is combined Figure 1-8 The present invention will be described in detail below. For ease of description, the directions referred to below are defined as follows: the directions of up, down, left, right, front, and back mentioned below are the same as... Figure 1 The directions of front, back, left, right, up, and down in the view are consistent. Figure 1 The directions shown are consistent with the front-to-back, left-to-right, up-down directions of the device of the present invention when viewed from the front.

[0032] Please see Figure 1-7This invention provides an embodiment 1: a magnetically linked non-contact driven closed card holder, comprising a base 1, a pipette 2, and a top cover 3. The base 1 has a central rotating axis and at least one chamber distributed in the circumferential direction for holding samples, reagents, waste liquids, etc., or for use in reactions and detections. The base 1 is connected to the bottom of the top cover 3, forming a sealed space between the base 1 and the interior of the top cover 3. The top cover 3 has an inner cavity 303, and an inner cavity 301 is provided at the center of the upper end of the top cover 3, thus forming a cylindrical inner recess within the inner cavity 303. The central hole of the pipette 2 is slidably fitted onto the cylindrical inner recess. The pipette 2 can rotate relative to the central axis of the base 1 and can be positioned above the chamber. A [missing information - likely a design feature] is provided on the upper end surface of the pipette 2 around the central hole. The support portion 201 has a rounded edge near the central hole. Four equidistant cavities 202 are arranged on the support portion 201, each containing a fixedly embedded magnetic component 204. A pipette tip 203 is fixedly mounted on the outer ring of the pipette fitting 2, allowing it to be inserted into the cavity. A limiting component for vertical positioning of the pipette fitting 2 is provided between the side of the pipette fitting 2 away from the pipette tip 203 and the side wall of the inner cavity 303 of the upper cover. An opening 302 is provided at the top of the upper cover 3, and a flexible tubing 5 connects the opening 302 to the pipette tip 203. A syringe located outside the cartridge can connect to the inner cavity of the pipette tip 203 through the opening 302, enabling actions such as liquid aspiration, dispensing, and mixing via the pipette tip 203.

[0033] An external driving mechanism is used to drive the drive rod 4 to rotate and move up and down. A disc portion 401 is fixedly provided at the bottom end of the drive rod 4. Magnetic elements 402 are fixedly distributed around the bottom of the disc portion 401 around the drive rod 4. The distribution of each magnetic element 402 corresponds to the magnetic element 204. Therefore, the end of the drive rod 4 with the magnetic element 402 is inserted into the concave cavity 301 as the magnetic end, so that the magnetic elements 402 and the magnetic element 204 generate a mutual magnetic attraction, thereby using magnetic force to control the movement. The rotation and axial movement of the liquid tube 2 form a non-contact drive, effectively ensuring the sealing of the cartridge. At the same time, it avoids the use of sealing rings and other mechanisms, greatly reducing the difficulty of sealing the internal space and reducing the risk of leakage. The friction is also much lower than that caused by the traditional pipette using sealing rings, which is conducive to reducing the size of the drive mechanism. At the same time, the precision of pipette movement control is greatly improved, effectively avoiding insufficient liquid aspiration, excessive liquid hanging at the tip, excessive residual liquid, and more serious problems such as unexpected collisions between the aspiration tube and other parts of the cartridge, which may lead to complete failure of the pipetting function.

[0034] It is worth mentioning that, in order to ensure that the drive rod 4 can fully drive the pipette 2 to move, the magnetic poles of the first magnetic component 204 and the second magnetic component 402 are opposite, and the interaction force between the first magnetic component 204 and the second magnetic component 402 is not less than 100mN·m in the rotation direction and not less than 5.0N in the vertical direction.

[0035] The aforementioned magnetic component 204 and magnetic component 402 can be permanent magnets or magnetically conductive materials, or coils that generate magnetic fields.

[0036] It should be noted that, in order to prevent the pipette fitting 2 from detaching from the cap 3, refer to... Figure 7 A limiting base 7 is installed at the bottom of the concave part of the cylinder to limit the position of the pipette 2.

[0037] refer to Figure 8 Example 2: The main difference from Example 1 is that the drive rod 4 is replaced with a drive ring 6. Magnetic elements 402 are also distributed on the drive ring 6. The movement of the pipette 2 is controlled by moving and rotating the drive ring 6.

[0038] The above description is merely a specific embodiment of the invention, but the scope of protection of the invention is not limited thereto. Any variations or substitutions conceived without inventive effort should be included within the scope of protection of the invention. Therefore, the scope of protection of the invention should be determined by the scope defined in the claims.

Claims

1. A magnetically linked non-contact driven closed card holder, comprising a base (1), a top cover (3), and a pipette fitting (2), characterized in that: The upper end face of the pipette fitting (2) is provided with a support portion (201) around the central hole. At least one magnetic element (204) is fixedly disposed on the support portion (201). A pipette tip (203) is fixedly disposed on the outer ring of the pipette fitting (2). The top of the upper cover (3) is provided with an opening (302). A flexible tube (5) is connected between the opening (302) and the pipette tip (203). The outer side of the upper cover (3) is magnetically attracted to the magnetic element (204) to drive the pipette fitting (2) to move. The support portion (201) is provided with grooves (202). The magnetic element (204) is fixedly embedded in the grooves. In the aforementioned cavity (202), the upper cover (3) is provided with an inner cavity (303), and an inner cavity (301) is provided at the center of the upper cover (3). The inner cavity (301) is used for the magnetic end of the driving mechanism to extend into. A cylindrical inner concave part is formed on the upper side wall of the inner cavity (303). The central hole of the pipette fitting (2) is slidably sleeved on the cylindrical inner concave part. The magnetic end of the driving mechanism includes a driving rod (4). A disk part (401) is fixedly provided at the bottom end of the driving rod (4). Magnetic element two (402) is fixedly distributed around the bottom of the disk part (401) around the driving rod. Each magnetic element two (402) corresponds to the magnetic element one (204).

2. The magnetically linked non-contact drive enclosed card holder according to claim 1, characterized in that: The base (1) is located at the bottom of the upper cover (3), and a sealed space is formed between the base (1) and the upper cover (3). The base (1) and the upper cover (3) are configured to be detachably connected or integrated.

3. The magnetically linked non-contact drive enclosed card holder according to claim 1, characterized in that: The magnetic component (204) is a permanent magnet or a magnetically conductive material, or a coil that generates a magnetic field.

4. The magnetically linked non-contact drive enclosed card holder according to claim 3, characterized in that: The magnetic poles of the first magnetic component (204) and the second magnetic component (402) are opposite, and the interaction force between the first magnetic component (204) and the second magnetic component (402) is not less than 100 mN·m in the rotational direction and not less than 5.0 N in the vertical direction.

5. A magnetically linked non-contact drive enclosed card holder according to claim 1, characterized in that: The edge of the bracket part (201) near the center hole is rounded.

6. A magnetically linked non-contact drive enclosed card holder according to claim 1, characterized in that: A limiting base (7) is fixedly installed at the bottom of the cylindrical concave part of the upper cover (3).

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