Vessel for transporting cells
By designing a funnel-shaped cover and sealing structure in the cell transport vessel, the problem of shear force damage to the cell membrane caused by the shaking of the culture medium during long-distance transport was solved, achieving a simple and economical shear force inhibition effect.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-27
- Publication Date
- 2026-03-31
AI Technical Summary
During long-distance cell transfer, the cell membrane is susceptible to shear force damage caused by the shaking of the culture medium. Existing shear force suppression devices are complex in structure and expensive.
Design a vessel comprising a main body, a lid, and a cover. The cover has a funnel-shaped structure to reduce the surface area of the culture medium. The design of through holes and openings within the cover suppresses the shaking of the culture medium. A sealing ring and a rubber stopper are used to ensure sealing and stability.
It effectively reduces shear damage to cells during transport, and has a simple structure and low manufacturing cost.
Smart Images
Figure CN224061594U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to a vessel for transporting cells. Background Technology
[0002] The cell membranes of certain biological cells (formulations) are very fragile. During cell transfer, especially long-distance transfer (e.g., long-distance transport), the cell membranes of cells in the vessel are easily damaged by shear forces caused by the shaking of the culture medium within the vessel. Although some vessels for suppressing shear forces have been developed in the prior art, for example, by arranging microchannels in the vessel to suppress the flow of the culture medium and reduce shear forces, this results in complex vessel structures and increased manufacturing costs. Utility Model Content
[0003] In view of the above-mentioned technical problems existing in the prior art, the embodiments of this utility model provide a vessel for transporting cells.
[0004] To solve the above-mentioned technical problems, the technical solution adopted in the embodiments of this utility model is as follows:
[0005] A vessel for transporting cells, comprising:
[0006] The main body of the vessel is used to hold the culture medium and cells;
[0007] A lid for attaching to the upper end of the vessel body;
[0008] A cover, configured as a flared structure with a lower port larger than the upper port, is fastened into the body of the vessel to suppress the shaking of the culture medium located within the cover.
[0009] Preferably, the vessel lid has a through hole, and the upper port of the cover protrudes from the vessel lid through the through hole.
[0010] Preferably, the upper port of the cover is provided with a rubber stopper, which has an elastic slit that can be sealed when closed. The suction device passes through the elastic slit and draws air above the liquid surface of the culture medium inside the cover to fill the cover with the culture medium.
[0011] Preferably, the lower port of the cover has an opening in its wall to allow culture medium from outside the cover to enter the interior of the cover.
[0012] Preferably, a grille is arranged at the opening.
[0013] Preferably, the upper part of the cover is provided with an annular step, and a sealing ring is arranged on the annular step. After the vessel lid is fastened to the vessel body, the vessel lid presses against the sealing ring.
[0014] Preferably, the wall of the vessel body is configured as an inclined wall such that the upper end of the vessel body is larger than the bottom of the vessel body.
[0015] Preferably, the vessel body is a circular vessel, and the vessel lid is screwed onto the vessel body.
[0016] Preferably, the vessel body and the cover are configured to be transparent.
[0017] Preferably, a sealing ring is provided between the lid of the vessel and the upper port of the vessel body.
[0018] Compared with the prior art, the beneficial effects of the cell transport vessel provided by the embodiments of this utility model are:
[0019] 1. By adding a cover to the main body of the vessel and configuring the cover into a funnel-shaped structure, the liquid surface area of the culture medium inside the cover can be significantly reduced, thereby suppressing the shaking of the culture medium and reducing the damage to the cells caused by excessive shear force generated by shaking during transportation.
[0020] 2. The vessel for suppressing shear force provided by this utility model has a simple structure, is easy to manufacture, and has a low manufacturing cost.
[0021] The overview of various implementations or examples of the technology described in this utility model is not a complete disclosure of the full scope or all features of the disclosed technology. Attached Figure Description
[0022] In drawings that are not necessarily drawn to scale, the same reference numerals may describe similar parts in different views. The same reference numerals with or without letter suffixes may indicate different instances of similar parts. The drawings generally illustrate various embodiments by way of example rather than limitation and, together with the description and claims, serve to explain embodiments of the utility model. Where appropriate, the same reference numerals are used in all drawings to refer to the same or similar parts. Such embodiments are illustrative and not intended to be exhaustive or exclusive embodiments of the apparatus or method.
[0023] Figure 1 A front sectional view of a cell transport vessel provided in one embodiment of this utility model.
[0024] Figure 2 for Figure 1 A magnified view of part A.
[0025] Figure 3 A main cross-sectional view of a cell transport vessel provided for another embodiment of this utility model.
[0026] In the picture:
[0027] 10-Vessel body; 11-Vessel body; 12-Vessel bottom; 20-Vessel lid; 21-Through hole; 30-Cover body; 31-Lower port; 32-Upper port; 33-Notch; 331-Grate; 34-Annular step; 35-Rubber stopper; 36-Elastic joint; 41-First sealing ring; 42-Second sealing ring. Detailed Implementation
[0028] Unless otherwise defined, the technical or scientific terms used in this utility model shall have the ordinary meaning understood by one of ordinary skill in the art to which this utility model pertains. The terms "first," "second," and similar terms used in this utility model do not indicate any order, quantity, or importance, but are merely used to distinguish different components. Terms such as "comprising" or "including" mean that the element or object preceding the word encompasses the elements or objects listed following the word and their equivalents, without excluding other elements or objects. Terms such as "connected" or "linked" are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. Terms such as "upper," "lower," "left," and "right" are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly.
[0029] To keep the following description of the embodiments of this utility model clear and concise, detailed descriptions of known functions and known components are omitted.
[0030] like Figure 1 and Figure 2 As shown, this utility model discloses a cell transport vessel, which includes: a vessel body 10, a vessel lid 20, and a cover 30. The vessel body 10, the vessel lid 20, and the cover 30 are all made of transparent material, such as inorganic glass or plexiglass.
[0031] The vessel body 10 is preferably circular and is used to hold cells and culture medium. The vessel lid 20 is used to fasten onto the upper port 32 of the vessel body 10 to seal the vessel body 10. Preferably, the vessel lid 20 is fastened onto the vessel body 10 by screwing. Preferably, a first sealing ring 41 is disposed between the vessel lid 20 and the upper port 32 of the vessel body 10 to prevent culture medium leakage.
[0032] The cover 30 is configured with a funnel-shaped structure, where the lower port 31 is much larger than the upper port 32. This results in a smaller cross-section in the middle of the cover 30. The size of the lower port 31 of the cover 30 is essentially the same as the size of the bottom 12 of the vessel body 10. This allows the cover 30 to be inserted into the vessel body 10 from above, so that the cells are covered by the cover 30. Thus, even if the liquid level of the culture medium inside the cover 30 is the same as the liquid level of the culture medium outside the cover 30 based on the principle of communicating vessels, the funnel-shaped structure of the cover 30 significantly reduces the surface area of the liquid medium inside the cover 30. This significantly suppresses the shaking of the culture medium inside the cover 30, thereby significantly suppressing the shear forces generated inside the cover 30 that could damage the cells. Preferably, the body 11 of the vessel body 10 is configured as a conical surface, which facilitates the insertion of the cover 30 into the vessel body 10 from the upper port 32.
[0033] In some embodiments, such as Figure 1 As shown, the upper port 32 of the cover 30 is located below the vessel lid 20. In some preferred embodiments, such as... Figure 2 As shown, the upper port 32 of the cover 30 extends above the vessel lid 20. Specifically, the vessel lid 20 has a through hole 21 in the middle, and the upper end of the cover 30 passes through the through hole 21. When the vessel lid 20 is screwed on, the vessel lid 20 applies pressure to the cover 30 to fix the cover 30, thereby limiting the shaking of the cover 30. In some more preferred embodiments, an annular step 34 is provided on the upper part of the cover 30, and a second sealing ring 42 is fitted on the annular step 34. The vessel lid 20 presses against the second sealing ring 42, and the second sealing ring 42 is used to limit the leakage of culture medium through the through hole 21 in the middle of the vessel lid 20.
[0034] In some preferred embodiments, the upper port 32 of the cover 30 is provided with a rubber stopper 35. The rubber stopper 35 has an elastic slit 36 that can be sealed when closed. The suction device passes through the elastic slit 36 and draws air above the liquid surface of the culture medium inside the cover 30, thereby filling the cover 30 with culture medium and completely removing the air inside the cover 30, thus further suppressing the shaking of the culture medium inside the cover 30. In some more preferred embodiments, such as Figure 1 and Figure 2 As shown, the lower port 31 of the cover 30 has six circumferentially arranged notches 33 on its wall to allow culture medium from outside the cover 30 to enter the interior of the cover 30, thereby facilitating the entry of culture medium from the vessel body 10 into the cover 30 during air aspiration. More preferably, a grid 331 is arranged at the notch 33 to restrict cell transfer from inside the cover 30 into the vessel body 10.
[0035] The advantages of the vessel provided by this utility model are:
[0036] 1. By adding a cover 30 to the main body 10 of the vessel and configuring the cover 30 into a funnel-shaped structure, the liquid surface area of the culture medium inside the cover 30 can be significantly reduced, thereby suppressing the shaking of the culture medium and reducing the damage to the cells caused by excessive shear force generated by shaking during transportation.
[0037] 2. The vessel for suppressing shear force provided by this utility model has a simple structure, is easy to manufacture, and has a low manufacturing cost.
[0038] Furthermore, although exemplary embodiments have been described in this invention, its scope includes any and all embodiments based on this invention that have equivalent elements, modifications, omissions, combinations (e.g., schemes involving intersections of various embodiments), adaptations, or alterations. Elements in the claims will be interpreted broadly based on the language used in the claims and are not limited to the examples described in this specification or during the implementation of this application, which will be interpreted as non-exclusive. Therefore, this specification and examples are intended to be considered illustrative only, and the true scope and spirit are indicated by the following claims and the full scope of their equivalents.
[0039] The above description is intended to be illustrative and not restrictive. For example, the above examples (or one or more of them) can be used in combination with each other. Other embodiments can be used by those skilled in the art when reading the above description. Furthermore, in the above detailed description, various features may be grouped together to simplify the invention. This should not be construed as an intention that a disclosed feature not claimed is necessary for any claim. Rather, the subject matter of the invention may be less than all the features of a particular disclosed embodiment. Thus, the following claims are incorporated herein by reference as examples or embodiments, wherein each claim is an independent, separate embodiment, and these embodiments are contemplated to be combined with each other in various combinations or arrangements. The scope of the invention should be determined by reference to the appended claims and the full scope of their equivalents.
[0040] The above embodiments are merely exemplary embodiments of this utility model and are not intended to limit this utility model. The scope of protection of this utility model is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to this utility model within its substance and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of this utility model.
Claims
1. A vessel for transporting cells, characterized in that, Comprising: a vessel body for containing culture medium and cells; a vessel cover for being screwed on the upper port of the vessel body; a cover body configured in a horn shape with the lower port being larger than the upper port, the cover body being screwed in the vessel body for inhibiting the shaking of the culture medium in the cover body.
2. The vessel for transporting cells according to claim 1, wherein The vessel cover is provided with a through hole, the upper port of the cover body protrudes out of the vessel cover through the through hole.
3. The vessel for transporting cells according to claim 2, wherein The upper port of the cover body is provided with a rubber plug having an elastic slit capable of being sealed after being closed, a suction device passes through the elastic slit to fill the cover body with the culture medium by sucking air above the liquid level of the culture medium in the cover body.
4. The vessel for transporting cells according to claim 1, wherein A gap is provided on the wall of the lower port of the cover body to allow the culture medium outside the cover body to enter the inside of the cover body.
5. The vessel for transporting cells according to claim 4, wherein A grid is arranged at the gap.
6. The vessel for transporting cells according to claim 2, wherein The upper part of the cover body is provided with an annular step, a sealing ring is arranged on the annular step, after the vessel cover is screwed on the vessel body, the vessel cover presses against the sealing ring.
7. The vessel for transporting cells according to claim 1, wherein The wall of the body of the vessel body is configured as an inclined wall so that the upper port of the vessel body is larger than the bottom of the vessel body.
8. The vessel for transporting cells according to claim 1, wherein The vessel body is a circular vessel, the vessel cover is screwed with the vessel body.
9. The vessel for transporting cells according to claim 1, wherein The vessel body and the cover body are configured to be transparent.
10. The vessel for transporting cells according to claim 1, wherein A sealing ring is arranged between the vessel cover and the upper port of the vessel body.