NK cell in-vitro amplification culture device
By introducing a transparent observation window and a sliding shelf structure into the NK cell in vitro expansion culture device, the problem of interference with the culture environment when observing cell status was solved, ensuring the stability of the culture dish and independent operation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-07
- Publication Date
- 2026-03-31
AI Technical Summary
Existing NK cell in vitro expansion and culture devices require opening the incubator to check cell status, which causes changes in humidity and temperature that affect other culture dishes and thus the cell culture effect.
An in vitro expansion and culture device for NK cells was designed, which adopts a transparent observation window and a sliding rack structure, allowing individual placement and removal of culture dishes and observation through the transparent observation window, avoiding the impact of opening the incubator on other culture dishes.
This allows for the independent operation and observation of petri dishes without opening the incubator, maintaining the stability of the culture environment and avoiding the impact of humidity and temperature changes on other petri dishes.
Smart Images
Figure CN224062785U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of NK cell in vitro expansion and culture technology, specifically to an NK cell in vitro expansion and culture device. Background Technology
[0002] NK cells are important immune cells in the body, not only involved in anti-tumor, anti-viral infection, and immune regulation, but also participating in hypersensitivity reactions and the occurrence of autoimmune diseases in certain situations. They can recognize target cells and kill mediators. They originate from bone marrow lymphoid stem cells, and their differentiation and development depend on the bone marrow and thymus microenvironment. They are mainly distributed in bone marrow, peripheral blood, liver, spleen, lungs, and lymph nodes. Unlike T and B cells, NK cells are a type of lymphocyte that can non-specifically kill tumor cells and virus-infected cells without prior sensitization. In the development of medicine, it has been found that NK cells can help the human body fight viruses. Therefore, medical researchers conduct experiments by culturing NK cells for in vitro expansion.
[0003] Some NK cell in vitro expansion and culture devices require opening the entire incubator to check the cultured cells during use. This can cause changes in the humidity and temperature inside the incubator, and factors such as exposure to external air humidity can affect the culture of cells in other culture dishes. Therefore, this does not meet the current requirements. To address this, we have proposed an NK cell in vitro expansion and culture device. Utility Model Content
[0004] The purpose of this invention is to provide an in vitro expansion and culture device for NK cells, in order to solve the problem that, when using the in vitro expansion and culture device for NK cells mentioned in the background art, it is necessary to open the entire incubator to check the condition of the cultured cells. This will cause changes in the humidity and temperature inside the incubator, and factors such as contact with external air humidity will affect the culture of cells in other culture dishes.
[0005] To achieve the above objectives, this utility model provides the following technical solution: an in vitro expansion and culture device for NK cells, comprising an incubator and a culture dish. The incubator has four compartments spaced apart inside, and a placement rack is slidably arranged inside each compartment, with the culture dish placed inside the placement rack. A transparent observation window is fixedly provided at the upper end of each compartment. A movable groove is provided on one side of each compartment, inside the incubator. A sliding plate is slidably connected inside the movable groove. A limiting plate is fixedly connected to the side of the sliding plate near the compartment. One end of the limiting plate extends through into the corresponding compartment and is movably connected to the corresponding placement rack.
[0006] Preferably, a connecting rod is fixedly connected to the inner wall of the movable tank on the side away from the partition, and a push rod extending through and into the interior of the movable tank is movably connected to the top of the incubator, with the bottom end of the push rod slidably connected to the outer surface of the connecting rod.
[0007] Preferably, a connecting block is fixedly connected to the bottom end of the sliding plate on the side away from the limiting plate, and a driving groove is provided at the upper end of the connecting block, with an inclined surface provided on the inner wall of the driving groove.
[0008] Preferably, a drive rod is fixedly connected to the bottom end of one side of the push rod, the bottom end of the drive rod extends into the interior of the drive groove, and the drive rod slides in cooperation with the inclined surface of the drive groove.
[0009] Preferably, the sliding plate is located on the side away from the connecting block, and springs are fixedly connected between the upper and lower sides of the limiting plate and the inner wall of the movable groove.
[0010] Preferably, a limiting groove is provided on the side of the placement rack near the movable groove, and one end of the limiting plate extending through into the partition groove is inserted into the corresponding limiting groove.
[0011] Compared with the prior art, the beneficial effects of this utility model are:
[0012] 1. This utility model pushes the push rod, causing the sliding groove inside the push rod to slide down along the outer surface of the connecting rod, which then drives the drive rod to move downward, and causes one side of the bottom end of the drive rod to slide down along the inclined surface of the drive groove. This pulls the sliding plate and causes the limiting plate to move away from the partition groove, causing the spring to stretch and causing one end of the limiting plate to disengage from the limiting groove, thus releasing the limitation on the placement rack. The placement rack can then be pulled out from inside the partition groove, and a culture dish containing NK cells can be placed inside the placement rack. The placement rack containing the culture dish is then pushed into the partition groove, and the push rod is released, causing the spring to release its elastic force, thus allowing the limiting plate to be inserted into the limiting groove, thereby fixing the placement rack inside the partition groove.
[0013] 2. This utility model avoids mutual interference when picking up individual culture dishes by placing the placement rack with the culture dishes separately inside each compartment, and facilitates observation of the culture dishes through the transparent observation window set at the top of each compartment. Attached Figure Description
[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model;
[0015] Figure 2 This is a schematic diagram of the structure of the placement rack of this utility model pulled out from the outside of the incubator;
[0016] Figure 3 This is a front sectional view of the incubator of this utility model;
[0017] Figure 4 This utility model Figure 3 A schematic diagram of the structure at point A in the middle.
[0018] In the diagram: 1. Incubator; 101. Divider; 102. Movable slot; 2. Placement rack; 201. Limiting slot; 3. Petri dish; 4. Transparent observation window; 5. Connecting rod; 6. Push rod; 601. Sliding slot; 7. Drive rod; 8. Sliding plate; 9. Limiting plate; 10. Spring; 11. Connecting block; 1101. Drive slot. Detailed Implementation
[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.
[0020] Please see Figures 1 to 4 This utility model provides an embodiment of an in vitro expansion and culture device for NK cells, including an incubator 1 and culture dishes 3. The incubator 1 has four partitions 101 spaced apart inside, and a placement rack 2 is slidably installed inside each partition 101. The culture dishes 3 are placed inside the placement rack 2. By placing the placement rack 2 with the culture dishes 3 separately inside each partition 101, the mutual interference when picking up the culture dishes 3 is avoided. A transparent observation window 4 is fixedly installed at the upper end of each partition 101. The culture dishes 3 can be easily observed through the transparent observation window 4 at the upper end of each partition 101. A movable groove 102 is opened on one side of each partition 101 and inside the incubator 1. A sliding plate 8 is slidably connected inside the movable groove 102. A limiting plate 9 is fixedly connected to the side of the sliding plate 8 near the partition 101. One end of the limiting plate 9 extends through into the corresponding partition 101 and is movably connected to the corresponding placement rack 2.
[0021] A connecting rod 5 is fixedly connected to the inner wall of the movable groove 102 on the side away from the partition groove 101. A push rod 6 is movably connected to the top of the incubator 1, extending through into the interior of the movable groove 102. The bottom end of the push rod 6 is slidably connected to the outer surface of the connecting rod 5. A connecting block 11 is fixedly connected to the bottom end of the sliding plate 8 on the side away from the limiting plate 9. A driving groove 1101 is opened at the upper end of the connecting block 11. The inner wall of the driving groove 1101 is provided with an inclined surface. A driving rod 7 is fixedly connected to the bottom end of the push rod 6 on one side. The bottom end of the driving rod 7 extends into the interior of the driving 1101. The driving rod 7 slides in cooperation with the inclined surface of the driving groove 1101. A spring 10 is fixedly connected between the sliding plate 8 on the side away from the connecting block 11 and the inner wall of the movable groove 102, both above and below the limiting plate 9.
[0022] By pushing the push rod 6, the sliding groove 601 inside the push rod 6 slides down along the outer surface of the connecting rod 5, which in turn drives the drive rod 7 to move downward, and causes one side of the bottom end of the drive rod 7 to slide down along the inclined surface of the drive groove 1101, thereby pulling the sliding plate 8 to move the limiting plate 9 away from the partition groove 101, causing the spring 10 to be stretched.
[0023] A limiting groove 201 is provided on the side of the placement rack 2 near the movable groove 102. One end of the limiting plate 9 extends through into the interior of the partition 101 and is inserted into the corresponding limiting groove 201. By pulling up the spring 10, one end of the limiting plate 9 is disengaged from the limiting groove 201, thus releasing the limitation on the placement rack 2. Then, the placement rack 2 can be pulled out from the interior of the partition 101, and the culture dish 3 containing NK cells is placed inside the placement rack 2. Next, the placement rack 2 with the culture dish 3 is pushed into the interior of the partition 101. Then, the push rod 6 is released, so that the spring 10 releases its elastic force, thereby allowing the limiting plate 9 to be inserted into the interior of the limiting groove 201, thus fixing the placement rack 2 inside the partition 101.
[0024] When using the NK cell in vitro expansion and culture device, pushing the push rod 6 causes the sliding groove 601 inside the push rod 6 to slide downwards along the outer surface of the connecting rod 5. This then drives the drive rod 7 downwards, causing one side of the bottom end of the drive rod 7 to slide downwards along the inclined surface of the drive groove 1101. This pulls the sliding plate 8, causing the limiting plate 9 to move away from the partition groove 101, thus stretching the spring 10 and disengaging one end of the limiting plate 9 from the limiting groove 201. This releases the restriction on the placement rack 2, allowing the placement rack 2 to be pulled out from inside the partition groove 101 and the device to be placed in place. A culture dish 3 containing NK cells is placed inside a placement rack 2. The placement rack 2 containing the culture dish 3 is then pushed into the compartment 101. The push rod 6 is then released, causing the spring 10 to release its elastic force, thereby allowing the limiting plate 9 to be inserted into the limiting groove 201. This fixes the placement rack 2 inside the compartment 101. By placing the placement rack 2 containing the culture dish 3 separately inside each compartment 101, the mutual interference when handling each culture dish 3 is avoided. The transparent observation window 4 at the top of each compartment 101 facilitates observation of the culture dish 3.
[0025] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.
Claims
1. An NK cell in vitro expansion culture device, comprising a culture box (1) and a culture dish (3), characterized in that: The inside interval of the incubator (1) is equipped with four grooves (101), the inside of each groove (101) is slidably equipped with a rack (2), and a culture dish (3) is arranged in the inside of the rack (2), the upper end of each groove (101) is fixedly equipped with a transparent observation window (4), one side of each groove (101) and located in the inside of the incubator (1) is equipped with a movable slot (102), the inside of the movable slot (102) is slidably connected with a sliding plate (8), one side of the sliding plate (8) close to the groove (101) is fixedly connected with a limiting plate (9), one end of the limiting plate (9) extends through to the inside of the corresponding groove (101), and is movably connected with the corresponding rack (2).
2. The NK cell in vitro expansion culture device according to claim 1, wherein: The inner wall of the side of the movable slot (102) away from the groove (101) is fixedly connected with a connecting rod (5), the top end of the incubator (1) is movably connected with a push rod (6) extending through to the inside of the movable slot (102), and the bottom end of the push rod (6) is slidably connected to the outer surface of the connecting rod (5).
3. The NK cell in vitro expansion culture device according to claim 2, characterized in that: The bottom end of the side of the sliding plate (8) away from the limiting plate (9) is fixedly connected with a connecting block (11), the upper end of the connecting block (11) is equipped with a driving slot (1101), and the inner wall of the driving slot (1101) is provided with an inclined surface.
4. The NK cell in vitro expansion culture device according to claim 3, characterized in that: The bottom end of one side of the push rod (6) is fixedly connected with a driving rod (7), the bottom end of the driving rod (7) extends to the inside of the driving slot (1101), and the driving rod (7) is slidably connected with the inclined surface of the driving slot (1101).
5. The NK cell in vitro expansion culture device according to claim 3, characterized in that: The side of the sliding plate (8) away from the connecting block (11) and located between the upper and lower of the limiting plate (9) and the inner wall of the movable slot (102) is fixedly connected with a spring (10).
6. The NK cell in vitro expansion culture device according to claim 1, wherein: The side of the rack (2) close to the movable slot (102) is equipped with a limiting slot (201), and one end of the limiting plate (9) extending through to the inside of the groove (101) is inserted into the corresponding limiting slot (201).