Nanometer groove plate for exosome capture

Abstract

The utility model relates to the technical field of detection plates, in particular to a nano groove plate for capturing exosomes. According to the technical scheme, the device comprises a supporting bottom support, a groove is formed in the upper surface of the supporting bottom support, a first capturing plate and a second capturing plate are arranged in the groove in a sliding mode, and a plurality of third capturing plates are arranged on one side of the second capturing plate; the capturing grooves are formed in the first capturing plate, the second capturing plate and the third capturing plate, hole grooves used for enhancing the exosome enrichment and capturing efficiency are formed in the bottom faces of the interiors of the capturing grooves, and the distance between every two adjacent hole grooves is about 200 nanometers. According to the utility model, the enrichment and capture efficiency of the exosome can be obviously improved, the structure effectively avoids the occupation and consumption of soluble protein on a plate capture antibody, and provides a more adaptive binding site for the exosome, so that the capture efficiency of the exosome is greatly improved, the detection error caused by low capture efficiency is reduced, and the detection accuracy is improved. And the requirement of high-sensitivity detection is met.

Description

Technical Field

[0001] This invention relates to the field of detection plate technology, and in particular to a nanogroove plate for exosome capture. Background Technology

[0002] Exosomes are membrane-bound vesicles with a particle size of 30-150 nm, secreted by cells. They carry cell surface receptors, proteins, RNA, and other biomolecules, enabling intercellular communication. Many molecules expressed on cell membranes also participate in tumor metastasis, progression, and drug sensitivity. Therefore, exosome vesicle membrane molecules and contents are important for companion diagnostics and liquid biopsies. Exosome enrichment methods mainly include ultracentrifugation, immunoprecipitation, and the traditional ELISA method. While ultracentrifugation is currently the most commonly used exosome isolation method, its complex operation and difficulty in obtaining high-purity exosomes affect subsequent analysis and detection. Immunoprecipitation uses specific antibodies to capture exosomes, which can improve enrichment efficiency to some extent, but its sensitivity and capture efficiency are still relatively low, and the operation is time-consuming, limiting its suitability. Existing capture plates are often used to detect soluble protein molecules, but these plates cannot distinguish between soluble and exosome membrane proteins. Conventional capture plates suffer from reduced exosome capture efficiency due to the occupation and consumption of capture antibodies by soluble proteins. Even when antibodies are captured, they are often lost during washing because exosome vesicles are not firmly bound to the flat plate, leading to unstable detection results and failing to meet the requirements for high sensitivity and accuracy. Therefore, this invention proposes a nanogrooved plate for exosome capture. Utility Model Content

[0003] The purpose of this invention is to address the problems in the prior art where capture plates are commonly used to detect soluble protein molecules, but such plates cannot distinguish between soluble and exosome membrane proteins; even if ordinary capture plates capture antibodies, they will be washed away during washing because the exosome vesicles are not firmly bound to the flat bottom plate, resulting in unstable detection results and difficulty in meeting the requirements of high sensitivity and high accuracy. Therefore, a 96-well nanogroove plate for exosome capture is proposed.

[0004] The technical solution of this utility model is as follows: a nanogroove plate for exosome capture, comprising: a supporting base, the upper surface of which is provided with a groove, a first capture plate and a second capture plate being slidably disposed inside the groove, and a plurality of third capture plates being disposed on one side of the second capture plate; a plurality of capture grooves being formed on the first capture plate, the second capture plate and the third capture plates, and the inner bottom surface of the capture grooves being provided with pore grooves for enhancing the enrichment and capture efficiency of exosomes, and the spacing between the pore grooves is approximately 200 nanometers.

[0005] Optionally, a positioning component for limiting the first capture plate, the second capture plate, and the third capture plate is snapped onto one side of the support base. The positioning component includes a baffle on one side of the support base, a first through groove on one side of the baffle, a guide groove on the inner wall of the first through groove, a guide block slidably disposed inside the guide groove, and a stop block fixedly disposed between the guide blocks.

[0006] Optionally, a second through groove is provided on one side of the support base, and the second through groove is connected to the groove. A push plate is slidably arranged inside the second through groove, and the push plate is fixedly connected to one end of the first capture plate, the second capture plate and the third capture plate.

[0007] Optionally, the first capture plate, the second capture plate, and the third capture plate are arranged side by side and located on the same horizontal plane.

[0008] Optionally, the upper surface of the support base is provided with numerical and alphabetical markings.

[0009] Optionally, two slots are provided on one side of the support base, and a card block is inserted into the inside of the slot. One end of the card block is fixedly connected to the baffle.

[0010] Optionally, a first magnetic strip is fixedly embedded at one end of the first capture plate, the second capture plate, the third capture plate, and the capture groove, and a second magnetic strip is fixedly embedded on one side of the stop block.

[0011] In summary, this application includes at least one of the following beneficial technical effects:

[0012] This invention features 200nm grooves within the capture channels on the upper surfaces of the first, second, and third capture plates. Exosomes are more easily captured by antibodies coated at the bottom of the wells and stably bound to these grooves. Soluble proteins, lacking antigenic epitopes that bind to the coated antibodies, cannot be captured. Furthermore, their smaller size makes them less likely to specifically bind to the grooves, thus reducing the occupation and consumption of plate-site capture antibodies by soluble proteins. This allows the characteristic marker antibodies for exosome capture to be used more extensively for binding, significantly improving exosome enrichment and capture efficiency. This structure effectively avoids competitive binding and consumption of plate-site capture antibodies by soluble proteins, while reducing exosome loss during washing. It provides more suitable binding sites for exosomes, thereby greatly improving exosome capture efficiency, reducing detection errors caused by low capture efficiency, and meeting the requirements for high-sensitivity detection.

[0013] Furthermore, this invention utilizes the baffle, first through groove, guide groove, guide block, and stop block in the positioning component to limit the positioning of the first, second, and third capture plates. This allows the capture plates to be disassembled into units, enabling flexible selection of single or multiple capture plate units for operation when processing different sample volumes or multiple sample types. This avoids unnecessary use of the entire capture plate, significantly improving the flexibility and resource utilization of the capture plate. Additionally, if a unit of the capture plate malfunctions or becomes contaminated, the entire capture plate does not need to be replaced; only the damaged unit needs to be disassembled and replaced. This significantly reduces equipment maintenance and time costs, effectively improving the continuity and efficiency of the detection work, and providing strong support for the smooth operation of exosome capture. Attached Figure Description

[0014] Figure 1 A three-dimensional diagram of a nanogroove plate for exosome capture is provided;

[0015] Figure 2 for Figure 1 A schematic diagram of the split structure;

[0016] Figure 3 for Figure 2 A schematic diagram of the split structure of the positioning component;

[0017] Figure 4 for Figure 2 A schematic diagram of the structure of the third capture plate.

[0018] Figure label:

[0019] 1. Support base; 2. Groove; 3. First capture plate; 4. Second capture plate; 5. Third capture plate; 6. Capture slot; 7. Hole groove;

[0020] 8. Positioning component; 81. Baffle; 82. First through slot; 83. Guide slot; 84. Guide block; 85. Stop block;

[0021] 9. Second through slot; 10. Push plate; 11. Number label; 12. Letter label; 13. Card slot; 14. Card block; 15. First magnetic strip; 16. Second magnetic strip. Detailed Implementation

[0022] The technical solution of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are some embodiments of this utility model, but not all embodiments.

[0023] The components of the present invention embodiments described and shown in the accompanying drawings can typically be arranged and designed in a variety of different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention.

[0024] Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0025] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0026] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0027] Example

[0028] like Figure 1 , Figure 2 and Figure 4 As shown, the present invention proposes a nanogroove plate for exosome capture, comprising: a support base 1, the upper surface of which is provided with numerical labels 11 and letter labels 12, which are vertically arranged to classify and mark the capture plate, thereby making it easier to obtain information on the position after capture; a groove 2 is formed on the upper surface of the support base 1, and a first capture plate 3 and a second capture plate 4 are slidably arranged inside the groove 2; a plurality of third capture plates 5 are arranged on one side of the second capture plate 4; the first capture plate 3, the second capture plate 4 and the third capture plate 5 are arranged side by side and located on the same horizontal plane, which facilitates the addition of samples to the capture plate.

[0029] Furthermore, multiple capture grooves 6 are formed on the first capture plate 3, the second capture plate 4, and the third capture plate 5. The diameter of the capture groove 6 is about 0.69 cm and the height is 1.4 cm. The capture groove 6 corresponds to the number 11 and the letter 12, so that the position of the capture groove 6 can be obtained by the number 11 and the letter 12. The bottom surface of the capture groove 6 is provided with a hole groove 7, and the spacing of the hole groove 7 is about 200 nanometers. The nano-sized hole groove 7 can significantly enhance the enrichment and capture efficiency of exosomes.

[0030] like Figure 2 and Figure 3 As shown, a positioning component 8 is snapped onto one side of the support base 1 to limit the first capture plate 3, the second capture plate 4, and the third capture plate 5. The positioning component 8 includes a baffle 81 on one side of the support base 1. A first through groove 82 is provided on one side of the baffle 81. A guide groove 83 is provided on the inner wall of the first through groove 82. A guide block 84 is slidably arranged inside the guide groove 83 to ensure that the guide block 84 moves stably according to the direction of the guide groove 83. A stop block 85 is fixedly arranged between the guide blocks 84 to ensure the stable displacement of the stop block 85, which facilitates the removal or limiting of the capture plate.

[0031] like Figure 1 , Figure 2 and Figure 4 As shown, a second through groove 9 is provided on one side of the support base 1, and the second through groove 9 is connected to the groove 2. A push plate 10 is slidably arranged inside the second through groove 9. The push plate 10 is fixedly connected to one end of the first capture plate 3, the second capture plate 4 and the third capture plate 5, so as to push out one end of the capture plate, thereby facilitating the pulling of the capture plate for picking up and putting down.

[0032] Secondly, two slots 13 are provided on one side of the support base 1. A block 14 is inserted into the slot 13. One end of the block 14 is fixedly connected to the baffle 81, which makes it easy to disassemble the baffle 81 and thus facilitates cleaning of the inside of the groove 2 on the upper surface of the support base 1.

[0033] Furthermore, a first magnetic strip 15 is fixedly embedded at one end of the first capture plate 3, the second capture plate 4, the third capture plate 5, and the capture groove 6, and a second magnetic strip 16 is fixedly embedded on one side of the stop block 85, which can increase the stability of the limit between the stop block 85 and the capture plate and prevent the capture plate from shifting.

[0034] In this embodiment, samples are added to the capture grooves 6 on the first capture plate 3, the second capture plate 4, and the third capture plate 5. Because the bottom surface of the capture grooves 6 has perforated grooves 7, exosomes, due to their relatively large size and ability to bind to the exosome-specific marker antibody CD9 coated on the bottom of the perforations, are more likely to bind stably to these grooves. Soluble proteins, because they cannot bind to the coated antibodies and are smaller in size, are less likely to specifically bind to the grooves, thus reducing the occupation and consumption of the capture antibodies by soluble proteins at the plate sites, allowing the capture antibodies to be used more effectively for binding exosomes.

[0035] The sample is placed in capture groove 6, allowing exosomes to continuously bind directly to the capture antibodies in pore groove 7. This eliminates the need for exosome separation, significantly shortening detection time and enabling rapid enrichment and capture of exosomes in plasma or other bodily fluid samples for downstream detection. It also reduces exosome loss during washing. In this process, pore groove 7 provides more suitable binding sites for exosomes, greatly improving exosome capture efficiency, reducing detection errors caused by low capture efficiency, and increasing detection sensitivity and detection values.

[0036] If a unit of the capture plate malfunctions, becomes contaminated, or needs to be disassembled individually, the stop block 85 between the baffles 81 is moved. The stop block 85 moves stably within the guide groove 83 via the guide block 84, simultaneously causing the first magnetic strip 15 to detach from the second magnetic strip 16 it was attracted to. The stop block 85 is then moved to the side of the capture plate that needs to be disassembled, and the capture plate is then pushed out of the groove 2 on the support base 1 by the push plate 10. The capture plate is disassembled unit by unit, and the damaged unit is removed and replaced. This eliminates the need to replace the entire capture plate, significantly reducing equipment maintenance and time costs, effectively improving the continuity and efficiency of the detection work, and ensuring the smooth operation of exosome capture.

[0037] The above specific embodiments are merely optional embodiments of this utility model. Based on the technical solution of this utility model and the relevant teachings of the above embodiments, those skilled in the art can make various alternative improvements and combinations to the above specific embodiments.

Claims

1. A nanogroove plate for exosome capture, characterized by, Include: Supporting the bottom (1), the upper surface of the supporting bottom (1) is provided with a groove (2), the inside of the groove (2) is provided with a first capture plate (3) and a second capture plate (4), one side of the second capture plate (4) is provided with a plurality of third capture plates (5); A plurality of capture grooves (6) are provided on the first capture plate (3), the second capture plate (4) and the third capture plate (5), the inside bottom surface of the capture groove (6) is provided with a hole groove (7) for enhancing the efficiency of exosome enrichment and capture, and the pitch of the hole groove (7) is about 200 nanometers.

2. The nanogroove plate for exosome capture of claim 1, wherein, The side of the supporting bottom (1) is provided with a positioning assembly (8) for limiting the first capture plate (3), the second capture plate (4) and the third capture plate (5), the positioning assembly (8) includes a baffle (81) on one side of the supporting bottom (1), one side of the baffle (81) is provided with a first through slot (82), the inner wall of the first through slot (82) is provided with a guide groove (83), the inside of the guide groove (83) is provided with a guide block (84), and the guide block (84) is fixedly provided with a stop block (85) between them.

3. The nanogroove plate for exosome capture of claim 1, wherein, The side of the supporting bottom (1) is provided with a second through slot (9), and the second through slot (9) is provided in communication with the groove (2), the inside of the second through slot (9) is provided with a push plate (10), and the push plate (10) is fixedly connected with one end of the first capture plate (3), the second capture plate (4) and the third capture plate (5).

4. The nanogroove plate for exosome capture of claim 1, wherein, The first capture plate (3), the second capture plate (4) and the third capture plate (5) are arranged side by side and located in the same horizontal plane.

5. The nanogroove plate for exosome capture of claim 1, wherein, The upper surface of the supporting bottom (1) is provided with a digital mark (11) and an alphabetical mark (12).

6. The nanogroove plate for exosome capture of claim 2, wherein, The side of the supporting bottom (1) is provided with two clamping grooves (13), the inside of the clamping groove (13) is provided with a clamping block (14), and one end of the clamping block (14) is fixedly connected with the baffle (81).

7. The nanogroove plate for exosome capture of claim 2, wherein, One end of the first capture plate (3), the second capture plate (4), the third capture plate (5) and the capture groove (6) is fixedly embedded with a first magnetic stripe (15), and one side of the stop block (85) is fixedly embedded with a second magnetic stripe (16).

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