Sample storage and treatment device for gene detection

Abstract

The utility model relates to the field of medical detection, provides a sample storage and treatment device for gene detection, and aims to solve the problems of inconvenience in storage, easiness in omission and risk of pollution of DNA (deoxyribonucleic acid) samples during treatment in the prior art, and the sample storage and treatment device comprises a shell, a puncher, a punching die and a storage positioning mechanism, the punching die is arranged on the shell; the storing and positioning mechanism is detachably connected to the punching die and used for storing and positioning the dried blood spot collection cards during punching. The storage positioning mechanism can store a plurality of dried blood spot collection cards after sampling and can also perform positioning; when the blood spot needs to be punched and cut out, the storage positioning mechanism is directly installed on the shell, and the puncher is used for punching. And through the matched design of the storage positioning mechanism and the punching die, the dried blood spot collection cards do not need to be sequentially taken out for punching during sample punching treatment, pollution to dried blood spot collection card samples for Rh blood group gene detection is effectively reduced, and more convenience is achieved. The method is especially suitable for large-scale Rh blood group gene screening.

Description

Technical Field

[0001] This utility model relates to the field of medical testing, and in particular to a sample storage and processing device for gene testing. Background Technology

[0002] The Rh blood group system is one of the most important blood group systems in humans, with its main antigen located on the Rh protein on the red blood cell membrane. Rh blood group gene testing is of great significance in fields such as transfusion medicine, prenatal diagnosis, and forensic medicine. By testing the Rh blood group gene, an individual's Rh blood type can be accurately determined, providing guidance for clinical blood transfusions and preventing the occurrence of immune diseases such as hemolytic disease of the newborn.

[0003] Establishing an Rh blood group gene bank using dried blood spot technology (also known as dried blood spot, DBS) offers significant advantages. Dried blood spot collection is a simple, non-invasive method for blood sample collection, allowing for long-term preservation of blood samples at room temperature, facilitating sample transportation and storage. Using dried blood spot technology to establish a gene bank not only saves on cold chain transportation and storage costs but also improves sample stability and traceability, which is beneficial for large-scale population genetic research and database construction.

[0004] However, the inventors discovered in their Rh blood group system gene establishment project that existing dried blood spot sample storage and processing devices have not been adapted to the high-throughput task of establishing an Rh blood group system gene bank. Firstly, traditional devices store multiple dried blood spot collection cards sequentially in plastic bags, resulting in a disorganized and difficult-to-order process, increasing the risk of omissions. Furthermore, processing requires individually removing the samples, punching holes, and cutting blood smears, which is cumbersome, time-consuming, and inefficient, while also increasing the risk of human error and DNA contamination. During the removal and processing, samples may be contaminated by external factors, leading to DNA degradation or cross-contamination, affecting the accuracy of Rh blood typing results. Utility Model Content

[0005] To address the aforementioned issues, this invention aims to provide a sample storage and processing device for gene detection. This device offers convenient storage, minimizing the risk of omissions, and eliminates the need for manual removal and punching of blood spot collection cards during processing. Instead, the cards are punched directly outside the storage device.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0007] This utility model provides a sample storage and processing device for gene detection, including a shell and a punch, and also includes a punching mold and a storage positioning mechanism; the punching mold is disposed on the shell; the storage positioning mechanism is detachably connected to the top of the punching mold and located below the punch, and is used for storing and positioning the dried blood spot collection card for gene detection during punching.

[0008] This design allows the storage and positioning mechanism to store and locate multiple dried blood spot collection cards after sampling. When it's necessary to punch holes to collect blood smears, the storage and positioning mechanism is simply installed onto the housing, and the puncher can then be used to punch holes. For Rh blood typing, ensuring the integrity of the sample's DNA information is paramount. The coordinated design of the storage and positioning mechanism and the punching mold eliminates the need to sequentially remove the dried blood spot collection cards for punching, effectively reducing contamination of the Rh blood typing samples and simplifying the process. This is particularly suitable for large-scale Rh blood typing screening.

[0009] Furthermore, the storage positioning mechanism includes a base plate and a cover plate; the base plate is provided with multiple positioning slots; the cover plate is rotatably connected to the base plate and is provided with multiple positioning blocks that correspond one-to-one with the positioning slots; wherein, both the positioning slots and the positioning blocks are provided with auxiliary holes corresponding to the sample area of ​​the dried blood spot collection card.

[0010] With this design, the positioning groove on the base plate and the positioning block on the cover plate work together to press and position the dried blood spot collection card when the base plate and cover plate are joined together; the reserved auxiliary hole is for subsequent drilling.

[0011] Furthermore, the aforementioned sample storage and processing device for gene detection also includes a sealing sleeve; a storage positioning mechanism is detachably fitted inside the sealing sleeve; and both the bottom plate and the cover plate are provided with stabilizing grooves that cooperate with the sealing sleeve.

[0012] With this design, the storage positioning mechanism is sealed after storing the dried blood spot collection card, further reducing contamination of the dried blood spot sample and degradation of the DNA carried by the sample.

[0013] Furthermore, the punching die is equipped with multiple hole dies, and each hole die corresponds to an auxiliary hole.

[0014] In some embodiments, the individual die is a protruding cylindrical shape. After the storage positioning mechanism is installed, the die is inserted into the auxiliary hole of the base plate, flush with the bottom of the positioning groove. This facilitates the stability of the storage positioning mechanism and also prevents the dried blood spot collection card from being pressed down during drilling.

[0015] Furthermore, the shell is slidably connected to a tube rack, which has multiple tube positions, and each tube position corresponds to a hole mold.

[0016] The tube rack is used to install centrifuge tubes and is located below the punching die. After punching, the blood smears cut from the dried blood spot collection card fall directly into the corresponding centrifuge tubes.

[0017] Furthermore, the housing is provided with an adjustment mechanism for adjusting the position of the punch.

[0018] In summary, this utility model has the following beneficial effects:

[0019] 1. The storage and positioning mechanism in this utility model is specifically designed for Rh blood typing. Its design ensures stable sample storage and avoids the risk of DNA degradation during storage. During sample processing, the storage and positioning mechanism can be directly installed onto the housing for perforation, eliminating the need to individually remove each dried blood spot collection card. This simplifies the process, reduces manual steps, and effectively lowers the risk of sample contamination. Especially in large-scale Rh blood typing screening, it significantly improves processing efficiency and accuracy.

[0020] 2. The storage positioning mechanism, used in conjunction with the sealing sleeve, effectively seals the dried blood spot collection card sample, preventing contamination from the external environment and protecting the DNA information in the sample. This ensures the accuracy of Rh blood type gene testing results even after long-term storage.

[0021] 3. The punching die and the auxiliary hole in the storage and positioning mechanism correspond one-to-one, and the die and the auxiliary hole fit precisely to ensure the accuracy of the punching operation, reduce the risk of deviation and sample damage, and thus improve the accuracy and reliability of sample processing.

[0022] 4. The tube rack designed to fit under the punching die allows the blood smears cut after punching to fall directly into the corresponding centrifuge tubes, reducing manual intervention, simplifying the operation process, and reducing possible errors from manual operation. Attached Figure Description

[0023] The accompanying drawings, which form part of this application, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings:

[0024] Figure 1 This is a schematic diagram of the sample storage and processing device for gene detection according to the present invention;

[0025] Figure 2 This is a schematic diagram of the structure of the shell of this utility model;

[0026] Figure 3 This is a structural schematic diagram of the housing of this utility model from another perspective;

[0027] Figure 4 This is a schematic diagram of the storage positioning mechanism of this utility model in its unfolded state;

[0028] Figure 5 This is a schematic diagram of the closed state of the storage positioning mechanism of this utility model;

[0029] Figure 6 This is a schematic diagram of the sealing sleeve of this utility model.

[0030] The components are: 1. Shell; 2. Puncher; 3. Punching die; 31. Hole mold; 4. Storage and positioning mechanism; 41. Base plate; 411. Positioning groove; 42. Cover plate; 421. Positioning block; 43. Auxiliary hole; 5. Sealing sleeve; 44. Stabilizing groove; 45. L-shaped limit buckle; 6. Pipe rack; 61. Pipe position. Detailed Implementation

[0031] To enable those skilled in the art to better understand the technical solution of this utility model, the technical solution of this utility model will be further described below in conjunction with the accompanying drawings and embodiments.

[0032] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "front end", "rear end", "inner side", "outer side", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0033] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set up," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances. Example 1

[0034] This embodiment provides a sample storage and processing device for gene detection, such as... Figures 1 to 6As shown, the device includes a housing 1 and a puncher 2, as well as a punching mold 3 and a storage and positioning mechanism 4. The punching mold 3 is mounted on the housing 1. The storage and positioning mechanism 4 is detachably connected to the top of the punching mold 3 and located below the puncher 2. It is used for storing and positioning dried blood spot collection cards for gene testing during punching. The storage and positioning mechanism 4 can store multiple dried blood spot collection cards after sampling and can also position multiple dried blood spot collection cards. When sample processing requires punching and cutting blood smears, the storage and positioning mechanism 4 is directly installed on the housing 1, and the puncher 2 can then punch the smears. For gene testing, it is important to ensure the integrity of the sample DNA information. The design of the storage and positioning mechanism 4 and the punching mold 3 eliminates the need to remove the dried blood spot collection cards one by one for punching when processing samples, effectively reducing contamination of dried blood spot collection card samples for Rh blood typing gene testing, while simplifying the process and making it more convenient. It is especially suitable for large-scale Rh blood typing gene screening.

[0035] Specifically, according to the requirements of sample testing, the punching die 3 is provided with a through hole for cutting blood smears of the corresponding specifications.

[0036] Furthermore, the storage and positioning mechanism 4 includes a base plate 41 and a cover plate 42. The base plate 41 has multiple positioning slots 411. The cover plate 42 is rotatably connected to the base plate 41 and has multiple positioning blocks 421 corresponding one-to-one with the positioning slots 411. Each positioning slot 411 and positioning block 421 has an auxiliary hole 43 corresponding to the sample area of ​​the dried blood spot collection card. The positioning slots 411 on the base plate 41 and the positioning blocks 421 on the cover plate 42 cooperate to press and position the dried blood spot collection card when the base plate 41 and cover plate 42 are closed. The reserved auxiliary holes 43 are for subsequent drilling. This makes storing the dried blood spot collection card particularly convenient. After collecting the sample from the dried blood spot collection card's sample area, the collection card is directly placed into the positioning slot 411, ensuring the sample area corresponds to the position of the auxiliary hole 43. Then, the cover plate 42 is closed, and the positioning blocks 421 correspondingly enter the positioning slots 411, thus achieving the positioning of the dried blood spot collection card. This ensures that the dried blood spot collection card is not only effectively positioned during storage, but also reserves the necessary space for drilling, ensuring the accuracy and efficiency of the drilling operation.

[0037] Furthermore, the aforementioned sample storage and processing device for gene detection also includes a sealing sleeve 5; a storage positioning mechanism 4 is detachably fitted inside the sealing sleeve 5; and stabilizing grooves 44 that cooperate with the sealing sleeve 5 are provided on both the base plate 41 and the cover plate 42. The storage positioning mechanism 4 is sealed after storing the dried blood spot collection card, further reducing contamination of the dried blood spot sample and degradation of the DNA carried by the sample.

[0038] Specifically, the base plate 41 and cover plate 42 of the storage positioning mechanism 4 are provided with stabilizing grooves 44 on both sides, and L-shaped limiting buckles 45 are also provided on the outer sides of the base plate 41 and cover plate 42. During installation, the protrusions corresponding to the stabilizing grooves 44 inside the sealing sleeve 5 slide within the stabilizing grooves 44 until the L-shaped limiting buckles 45 reach the edge of the sealing sleeve 5. The friction between the L-shaped limiting buckles 45 and the edge of the sealing sleeve 5 is preset to prevent the sealing sleeve 5 from easily sliding out.

[0039] Furthermore, the punching die 3 is provided with multiple perforated dies 31, each corresponding to an auxiliary hole 43. The cooperation between the punching die 3 and the storage positioning mechanism 4 ensures the accuracy of punching. Each perforated die 31 corresponds to an auxiliary hole 43, ensuring that during punching, the perforated die 31 can be accurately aligned with the position in the auxiliary hole 43, thereby avoiding punching deviation or sample damage. This design effectively improves operational accuracy, ensures accurate blood smear cutting, and reduces possible errors. The perforated die 31 is typically cylindrical, and its protruding shape facilitates insertion into the auxiliary hole 43 of the base plate 41, ensuring that the perforated die 31 is flush with the bottom of the positioning groove 411, thus ensuring the stability of the sample during punching, preventing sample displacement or downward pressure during punching, and ensuring smooth sample processing.

[0040] Furthermore, the housing 1 is slidably connected to a tube rack 6, which has multiple tube positions 61, each corresponding to a perforation mold 31. The tube rack 6, located below the perforation mold 3, is used to install centrifuge tubes. After punching, the blood smears cut from the dried blood spot collection card fall directly into the corresponding centrifuge tube. This facilitates subsequent sample processing, allowing the cut blood smears to fall directly into the designated tube position 61 without manual intervention, reducing operational complexity. The sliding connection of the tube rack 6 allows for flexible adjustment of different tube positions 61 to adapt to different work requirements, further improving the operability and flexibility of the equipment. Example 2

[0041] Based on Embodiment 1, in Embodiment 2, preferably, the housing 1 is provided with an adjustment mechanism for adjusting the position of the punch 2. In this embodiment, one end of the adjustment mechanism is slidably connected to the housing 1, and the other end is connected to the punch 2. Specifically, the adjustment mechanism includes a first telescopic rod slidably connected to the housing 1, a second telescopic rod rotatably connected to the first telescopic rod, and the punch 2 rotatably connected to the second telescopic rod. This two-section rotatable telescopic connection structure allows the punch 2 to flexibly adjust its position according to different operational needs, ensuring the flexibility of the punching operation. In other embodiments, the adjustment mechanism can also be configured with other adjustable length and direction structures.

[0042] Specific application examples:

[0043] Rh blood typing is widely used in blood type identification, clinical typing, prenatal health checkups, and infant D antigen screening. Rh blood typing typically requires perforating dried blood spots to extract DNA information. During the inventor's project, "Establishment and Clinical Application of Rh Blood Group System Gene Bank and Accidental Antibody Library," the efficient and accurate processing of large batches of samples became crucial due to increasing testing demands. Traditional manual processing methods are not only inefficient but also risk sample contamination or DNA degradation, affecting the accuracy of test results. In this case, the sample storage and processing device for gene testing provided by this invention can be used. The following is the usage procedure:

[0044] 1. Prepare samples:

[0045] First, after collecting dried blood spots, place the dried blood spot collection card on the base plate 41 of the storage and positioning mechanism 4, ensuring that the sample area corresponds to the auxiliary hole 43 of the base plate 41. Each dried blood spot collection card is placed into the designated positioning slot 411, the cover plate 42 covers the base plate 41, and the positioning block 421 enters the corresponding positioning slot 411 to securely position the collection card.

[0046] 2. Store in a sealed container:

[0047] The storage positioning mechanism 4 is installed into the sealing sleeve 5. The stabilizing groove 44 and the L-shaped limiting buckle 45 ensure the storage positioning mechanism 4 is stably installed within the sealing sleeve 5, maintaining the sample's airtightness. This effectively reduces external environmental contamination of the sample and protects the DNA within it.

[0048] 3. Drilling process:

[0049] The storage positioning mechanism 4 inside the sealing sleeve 5 is installed onto the punching die 3. Through this cooperation, the die 31 and the auxiliary hole 43 correspond one-to-one, ensuring that the die 31 is accurately aligned with the sample area during the punching process. The blood smear is cut by punching with the punch 2, ensuring that the size and position of the blood smear meet the requirements.

[0050] 4. Blood smear collection:

[0051] After punching, the blood smear will fall directly into the designated centrifuge tube in the tube rack 6 located below the punching die 3. The tube positions 61 on the tube rack 6 correspond one-to-one with the punching die 31, ensuring that each blood smear is accurately collected into the corresponding centrifuge tube.

[0052] 5. Follow-up processing:

[0053] After completing the above steps, the sample can be processed according to the dried blood paper method (also known as dried blood spot technology) and then proceed with the Rh blood type gene testing.

[0054] This method enables efficient and accurate storage, location, and processing of large batches of samples, avoiding the errors and contamination risks associated with traditional manual operations. It simplifies the workflow, improves testing efficiency, and effectively enhances the integrity of DNA in samples, making it particularly suitable for large-scale Rh blood group gene screening and meeting the high-throughput requirements for establishing and improving the Rh blood group gene bank.

[0055] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A sample storage and processing device for genetic testing comprising a housing (1) and a puncher (2), characterized in that, Also include: Punching die (3), provided on the shell (1); Storage positioning mechanism (4) is detachably connected to the top of the punching die (3), and is located below the puncher (2), which is used for storing and positioning the gene detection dry blood spot collection card during punching.

2. The sample storage and processing device for genetic testing of claim 1, wherein, The storage positioning mechanism (4) comprises: Bottom plate (41) provided with a plurality of positioning grooves (411); Cover plate (42), rotationally connected to the bottom plate (41), provided with a plurality of positioning blocks (421) corresponding to the positioning grooves (411) one by one; Wherein, the positioning groove (411) and the positioning block (421) are provided with auxiliary holes (43) corresponding to the dry blood spot collection card sample area.

3. The sample storage and processing device for genetic testing of claim 2, wherein, The sample storage and processing device further comprises a sealing sleeve (5); The storage positioning mechanism (4) is detachably provided in the sealing sleeve (5).

4. The sample storage and processing device for genetic testing of claim 2, wherein, The punching die (3) is provided with a plurality of hole dies (31), and the hole dies (31) correspond to the auxiliary holes (43) one by one.

5. The sample storage and processing device for genetic testing of claim 4, wherein, The shell (1) is slidably connected with a pipe rack (6), the pipe rack (6) is provided with a plurality of pipe positions (61), and the pipe positions (61) correspond to the hole dies (31) one by one.

6. The sample storage and processing device for genetic testing of claim 4, wherein, The shell (1) is provided with an adjusting mechanism for adjusting the position of the puncher (2).

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