Culture dish for culturing and transplanting unfrozen embryos
By setting partitions inside the embryo culture dish to divide the area into embryo culture and transfer areas, the problem of decreased embryo viability caused by temperature and humidity changes during transfer after thawing is solved. This allows culture and transfer to be completed in one dish, improving the success rate and reliability.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-24
- Publication Date
- 2026-04-03
AI Technical Summary
Embryos are susceptible to temperature and humidity changes during transfer after thawing, which affects the success rate of embryo transfer. This impacts the success rates of embryo culture and transfer in existing technologies, particularly in embryo culture dishes. Furthermore, embryo transfer procedures, especially after thawing, can lead to decreased embryo viability during transfer in embryo culture dishes, thus affecting the overall success rate.
Design a culture dish for culturing and transferring embryos after thawing. The dish is equipped with a partition to divide the internal area into an embryo culture area and an embryo transfer area. The lid and the dish body work together to ensure the independence and stability of the two areas and reduce the impact of environmental crossover.
When embryo culture and transfer are performed in a single dish, the existing technology improves embryo viability and transfer success rate, reduces the chance of errors during embryo transfer between different dishes, and improves reliability.
Smart Images
Figure CN224077427U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of medical and experimental equipment technology, and in particular to a culture dish for culturing and transplanting embryos after thawing. Background Technology
[0002] In assisted reproductive technology, the brief culture and transfer of embryos after thawing are crucial steps. The standard procedure involves briefly culturing the thawed embryos in a microdrop culture medium covered with mineral oil, and equilibrating them in a CO2 incubator one day in advance. Before embryo transfer, the embryos are transferred to a new transfer dish containing a small amount of transfer fluid, but not covering the embryo.
[0003] Currently, before embryo transfer, embryos need to be transferred from a culture dish to another transfer dish. This process increases the time spent handling the embryos in vitro, making them more susceptible to changes in temperature and humidity, which may lead to decreased embryo viability and thus affect the success rate of transfer. Furthermore, traditional culture dishes and procedures cannot solve the problems currently existing in the culture and transfer of thawed embryos. Utility Model Content
[0004] Based on the technical problems existing in the background art, this utility model proposes a culture dish for culturing and transplanting embryos after thawing.
[0005] This utility model proposes a culture dish for culturing and transplanting embryos after thawing, comprising a dish body and a dish lid. A partition is provided in the dish body to divide the internal area of the dish body into an embryo culture area and an embryo transplantation area. The plate of the partition is in contact with the inner wall of the dish body, and both the embryo culture area and the embryo transplantation area are in close contact with the inner wall of the dish body. The upper surface of the partition plate is in contact with the inner top wall of the dish lid.
[0006] Preferably, the outer periphery of the dish is provided with a rough texture first, and the outer periphery of the dish lid is provided with a rough texture second.
[0007] Preferably, the area of the embryo culture region is not less than the area of the embryo transfer region.
[0008] Preferably, the partition is a circular or polygonal tubular structure, and part of the partition is attached to the inner wall of the dish.
[0009] Preferably, the partition is a straight, arc-shaped, or S-shaped plate structure, with both ends of the partition tightly attached to the inner wall of the vessel.
[0010] Preferably, a limiting plate one is provided on both sides of the partition, and a limiting plate two and a limiting plate three are provided on the inner top wall of the lid. The limiting plate one, the limiting plate two, and the limiting plate three have the same shape and structure as the partition but are different in size.
[0011] Preferably, the partition is engaged between the second and third limiting plates and is in contact with the top wall between the second and third limiting plates inside the lid.
[0012] Preferably, the upper end face of the two limiting plates is respectively attached to the lower end face of the limiting plate and the limiting plate.
[0013] Preferably, a gap is provided between the limiting plate and the partition.
[0014] The beneficial effects of this utility model are as follows:
[0015] This invention relates to a culture dish for culturing and transferring thawed embryos. By setting a partition inside the dish and dividing the internal area into an embryo culture area and an embryo transfer area, it can provide a stable and suitable survival environment for embryo culture and transfer after thawing. The entire process can be completed in one dish, saving time and effort, improving embryo viability and transfer success rate, reducing the impact of environmental changes such as temperature and humidity on embryos, and reducing the chance of errors during embryo transfer between different dishes, thus ensuring high reliability. Attached Figure Description
[0016] Figure 1 : A schematic diagram of the structure of the petri dish in Embodiment 1 of this utility model;
[0017] Figure 2 : A schematic diagram of the structure of the petri dish in Embodiment 2 of this utility model;
[0018] Figure 3 : A schematic diagram of the structure of the dish lid in Embodiment 2 of this utility model;
[0019] Figure 4 : A schematic diagram of the structure of the petri dish in Embodiment 3 of this utility model;
[0020] Figure 5 : A schematic diagram of the structure of the dish lid in Embodiment 3 of this utility model;
[0021] Figure 6 : A schematic diagram of the structure of the petri dish in Embodiment 4 of this utility model;
[0022] Figure 7 : A schematic diagram of the structure of the dish lid in Embodiment 4 of this utility model. Detailed Implementation
[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.
[0024] Example 1:
[0025] like Figure 1 As shown, this utility model proposes a culture dish for culturing and transplanting embryos after thawing, comprising a dish body 1 and a dish lid 2. The outer periphery of the dish body 1 is provided with a rough texture 15, and the outer periphery of the dish lid 2 is provided with a rough texture 21. The rough texture 15 on the outer periphery of the dish body 1 and the rough texture 21 on the outer periphery of the dish lid 2 can increase the friction when the operator holds the dish body 1 and the dish lid 2, making it easier to operate.
[0026] A cylindrical partition 11 is provided inside the dish 1. The outer wall of the cylindrical partition 11 is attached to the inner wall of the dish 1, dividing the interior of the dish 1 into an embryo culture area 12 and an embryo transfer area 13. The area of the circular embryo culture area 12 is not less than the area of the embryo transfer area 13, and both the embryo culture area 12 and the embryo transfer area 13 are in close contact with the inner wall of the dish 1. Even though the embryo transfer area 13 is in close contact with one side of the interior of the dish 1, it ensures that the area of the embryo culture area 12 is large enough and concentrated, which is convenient for operation.
[0027] The embryo culture area 12 contains several microdroplets made with culture medium and covered with mineral oil for culturing thawed embryos; while the embryo transfer area 13 is filled with a certain amount of transfer fluid for later embryo transfer. After the dish lid 2 and dish body 1 are closed, the upper surface of the partition 11 adheres to the inner top wall of the dish lid 2, reducing environmental flow between the embryo culture area 12 and the embryo transfer area 13 and preventing cross-contamination.
[0028] Example 2:
[0029] Reference Figure 2-3 This invention proposes a culture dish for culturing and transplanting embryos after thawing, comprising a dish body 1 and a dish lid 2. The outer periphery of the dish body 1 is provided with a rough texture 15, and the outer periphery of the dish lid 2 is provided with a rough texture 21. The rough texture 15 on the outer periphery of the dish body 1 and the rough texture 21 on the outer periphery of the dish lid 2 can increase the friction when the operator holds the dish body 1 and the dish lid 2, making it easier to handle.
[0030] A partition 11 is installed inside the dish body 1, dividing the internal area of the dish body 1 into an embryo culture area 12 and an embryo transfer area 13. The area of the embryo culture area 12 is not smaller than the area of the embryo transfer area 13. The embryo culture area 12 contains several microdroplets made with culture medium and is covered with mineral oil for culturing thawed embryos. The embryo transfer area 13 is filled with a certain amount of transfer fluid for later embryo transfer. After the dish lid 2 is closed with the dish body 1, the upper surface of the partition 11 adheres to the inner top wall of the dish lid 2, reducing environmental flow between the embryo culture area 12 and the embryo transfer area 13 and preventing cross-contamination.
[0031] Limiting plates 14 are provided on both sides of the partition 11, with a gap between the limiting plates 14 and the partition 11. Limiting plates 22 and 23 are provided on the inner top wall of the lid 2. The limiting plates 14, 22, and 23 have the same shape and structure as the partition 11, but are different in size. After the lid 2 and the body 1 are closed, the partition 11 is engaged between the limiting plates 22 and 23 and is in contact with the top wall between the limiting plates 22 and 23 inside the lid 2. The upper surfaces of the two limiting plates 14 are in contact with the lower surfaces of the limiting plates 22 and 23, respectively. Therefore, during embryo culture and transfer, the combination of limiting plate 14 and limiting plate 22, and limiting plate 14 and limiting plate 33 again ensures the independence of the environment between the embryo culture area 12 and the embryo transfer area 13. Even if some water vapor seeps in, it will condense in the gap between limiting plate 14 and partition 11.
[0032] Partition 11, limiting plate 14, limiting plate 22, and limiting plate 3 23 are all tubular structures of closed polygons, such as circles, triangles, quadrilaterals, or other regular or irregular shapes. The outer wall of limiting plate 14 is attached to the inner wall of the dish body 1, and the outer wall of limiting plate 3 23 is attached to the inner wall of the dish lid 2.
[0033] like Figure 2-3 As shown in the figure, partition 11, limiting plate one 14, limiting plate two 22, and limiting plate three 23 are all cylindrical tubular structures. Partition 11 is attached to the inner wall of dish 1 through the cylindrical limiting plate one 14 on its outer side, and the outer wall of cylindrical limiting plate three 23 is attached to the inner wall of dish lid 2. The circular area enclosed by the limiting plate one 14 on the inner side of partition 11 is the embryo transfer area 13, and the annular area enclosed by the limiting plate one 14 on the outer side of partition 11 and the inner wall of dish 1 is the embryo culture area 12. Both the embryo culture area 12 and the embryo transfer area 13 are in close contact with the inner wall of dish 1, so that the embryo transfer area 13 is in close contact with the inside of dish 1, ensuring that the area of the embryo culture area 12 is large enough and concentrated, which is convenient for operation.
[0034] Example 3:
[0035] Reference Figure 4-5 The present invention proposes a culture dish for culturing and transplanting embryos after thawing, the structure of which is basically the same as that of Example 2, except that:
[0036] like Figure 4-5As shown, partition 11, limiting plate one 14, limiting plate two 22, and limiting plate three 23 are all linear plate-like structures. Partition 11 has straight ends. The two ends of partition 11 and limiting plate one 14 inside the dish body 1 are tightly attached to the inner wall of the dish body 1. The two ends of limiting plate two 22 and limiting plate three 23 inside the dish lid 2 do not contact the inner wall of the dish lid 2. The gaps between the two ends of limiting plate two 22 and limiting plate three 23 and the inner wall of the dish lid 2 are used for locking the outer periphery of the dish body 1. The smaller area enclosed by the limiting plate one 14 on the inner side of partition 11 and the inner wall of the dish body 1 is the embryo transfer area 13. The larger area enclosed by the limiting plate one 14 on the outer side of partition 11 and the inner wall of the dish body 1 is the embryo culture area 12. Both the embryo culture area 12 and the embryo transfer area 13 are tightly attached to the inner wall of the dish body 1. Even though the embryo transfer area 13 is tightly attached to one side of the inside of the dish body 1, this ensures that the area of the embryo culture area 12 is sufficiently large and concentrated, facilitating operation.
[0037] Example 4:
[0038] Reference Figure 6-7 The present invention proposes a culture dish for culturing and transplanting embryos after thawing, the structure of which is basically the same as that of Example 2, except that:
[0039] Partition 11, limiting plate 14, limiting plate 22, and limiting plate 3 23 are all arc-shaped or S-shaped plate structures. Specifically, such as... Figure 6-7 As shown, partition 11, limiting plate one 14, limiting plate two 22, and limiting plate three 23 are all arc-shaped plate structures of partition 11. The two ends of partition 11 and limiting plate one 14 inside the dish body 1 are tightly attached to the inner wall of the dish body 1, while the two ends of limiting plate two 22 and limiting plate three 23 inside the dish lid 2 do not contact the inner wall of the dish lid 2. The gaps between the two ends of limiting plate two 22 and limiting plate three 23 and the inner wall of the dish lid 2 are used for locking the outer periphery of the dish body 1. The smaller area enclosed by the limiting plate one 14 on the inner side of partition 11 and the inner wall of the dish body 1 is the embryo transfer area 13. The larger area enclosed by the limiting plate one 14 on the outer side of partition 11 and the inner wall of the dish body 1 is the embryo culture area 12. Both the embryo culture area 12 and the embryo transfer area 13 are tightly attached to the inner wall of the dish body 1. Even though the embryo transfer area 13 is tightly attached to one side of the inside of the dish body 1, this ensures that the area of the embryo culture area 12 is sufficiently large and concentrated, facilitating operation.
[0040] As shown in Examples 1, 2, 3, and 4, this utility model provides a culture dish for culturing and transplanting embryos after thawing. By setting a partition 11 inside the dish body 1 and dividing the internal area of the dish body 1 into an embryo culture area 12 and an embryo transplantation area 13, it can provide a stable and suitable survival environment for the culture and transplantation of embryos after thawing. The operation can be completed in one dish, saving time and effort, improving embryo viability and transplantation success rate, reducing the impact of environmental changes such as temperature and humidity on embryos, and reducing the chance of errors during embryo transfer between different dishes, thus ensuring high reliability.
[0041] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.
Claims
1. A culture dish for culture and transplantation of embryos after thawing, comprising a dish body (1) and a dish cover (2), characterized in that, The tray body (1) is provided with a partition plate (11) which separates the inner region of the tray body (1) into an embryo culture region (12) and an embryo transplantation region (13), the plate body of the partition plate (11) is in contact with the inner wall of the tray body (1) and makes the embryo culture region (12) and the embryo transplantation region (13) both closely adhere to the inner wall of the tray body (1), and the upper end surface of the partition plate (11) is in close contact with the inner top wall of the tray cover (2).
2. The culture dish for culture and transfer of thawed embryos according to claim 1, wherein The outer periphery of the tray body (1) is provided with rough lines (15), and the outer periphery of the tray cover (2) is provided with rough lines (21).
3. The culture dish for the culture and transfer of embryos after thawing according to claim 1, wherein The area of the embryo culture region (12) is not less than the area of the embryo transplantation region (13).
4. The culture dish for the culture and transfer of embryos after thawing according to claim 1, wherein The partition plate (11) is a tubular structure with a circular or polygonal shape, and part of the plate body of the partition plate (11) is in contact with the inner wall of the tray body (1).
5. The culture dish for the culture and transplantation of embryos after thawing according to claim 1, wherein The partition plate (11) is a plate structure with a straight line type, an arc type or an S type, and the two ends of the partition plate (11) are closely attached to the inner wall of the tray body (1).
6. The culture dish for culture and transfer of thawed embryos according to claim 4 or 5, wherein The two sides of the partition plate (11) are provided with limiting plates (14), the inner top wall of the tray cover (2) is provided with limiting plates (22) and (23), and the limiting plates (14), (22) and (23) have the same shape structure as the partition plate (11) but different sizes.
7. The culture dish for culture and transfer of thawed embryos according to claim 6, wherein The partition plate (11) is clamped between the limiting plates (22) and (23) and is in contact with the top wall between the limiting plates (22) and (23) of the tray cover (2).
8. The culture dish for culture and transfer of thawed embryos according to claim 6, wherein The upper end surfaces of the two limiting plates (14) are respectively in contact with the lower end surfaces of the limiting plates (22) and (23).
9. The culture dish for the culture and transplantation of embryos after thawing according to claim 6, wherein A gap is provided between the limiting plate (14) and the partition plate (11).