Plant non-in-vitro rooting device suitable for field

Abstract

The utility model relates to the technical field of plant rooting, in particular to a plant non-in-vitro rooting device suitable for the field, which comprises a non-in-vitro rooting device main body, a shading layer, a rooting capsule placing groove, a branch guide support, a bandage and a fixing component, the surface of the non-in-vitro rooting device main body is connected with a shading layer, and a rooting capsule placing groove is fixed on the inner wall of the non-in-vitro rooting device main body; branches are guided to be naturally bent through the S-shaped symmetrical branch guide supports, rooting space is reserved, the tightness of the branches can be adjusted through cooperation of the bandages and the positioning pieces, the branches can be matched with the branches with different thicknesses, rapid locking is achieved through control bolts in the fixing assemblies, and on the premise that parent nutrition transmission is reserved, the device is convenient to use and high in practicability. The problem that the survival rate of a traditional field rooting technology is low is solved, it is guaranteed that the to-be-rooted branches can continuously obtain water and nutrients, and the rooting survival rate is remarkably increased.

Description

Technical Field

[0001] This utility model relates to the field of plant rooting technology, and in particular to a non-in vitro plant rooting device suitable for use in the field. Background Technology

[0002] The root system is the "nutrient highway" of a plant. Its surface area can be 10-15 times that of the above-ground parts. Through active transport by the root tip and passive absorption by the root hair, the root system can transport water equivalent to 20 times its own weight every day. At the same time, it can accurately capture trace elements in the soil solution at the ppm level. Rooting devices are often used to cultivate plants in order to promote the spread of vegetation.

[0003] Existing plant rooting devices involve separating branches from the parent plant and placing them into the device for rooting. However, this method not only relies on a sterile laboratory environment but also cannot continuously supply organic matter and water from the parent plant to the rooting site, thus greatly reducing the rooting survival rate.

[0004] Therefore, in view of the problem that the traditional field rooting device uses in vitro rooting, which easily leads to low survival rate, a non-in vitro rooting device suitable for the field can be designed. Utility Model Content

[0005] To overcome the problem that traditional field rooting devices, which use in vitro rooting methods, often result in low survival rates.

[0006] The technical solution of this utility model is as follows: a non-in vitro rooting device for plants suitable for outdoor use, comprising a non-in vitro rooting device body, a light-shielding layer, a rooting capsule placement groove, a branch guide support, a binding strap, and a fixing component. A support base is fixed to the lower end of the non-in vitro rooting device body. A light-shielding layer is connected to the surface of the non-in vitro rooting device body. A rooting capsule placement groove is fixed to the inner wall of the non-in vitro rooting device body. A support is fixed to the bottom end of the inner wall of the non-in vitro rooting device body. A support rod is fixed to the upper end of the support rod. A branch guide support is fixed to the upper end of the non-in vitro rooting device body. A protective pad is connected to the upper end of the branch guide support. A binding strap is fixed to the rear end of the branch guide support. A positioning device is fixed to the front end of the binding strap. A fixing assembly is provided at the front end of the branch guide support. The fixing component includes control bolts.

[0007] Preferably, the support base provides stable support for the main body of the non-in vitro rooting device, the light-blocking layer effectively blocks direct sunlight and protects the new roots, the internal rooting capsule placement slot facilitates the placement of fertilizers containing growth hormones or nutrients to promote root development, the S-shaped symmetrical branch guide can guide the branches to bend naturally, and the fixed components facilitate fixing the branches in the device to prevent displacement or falling off.

[0008] Preferably, the light-shielding layer is a ring-shaped structure bonded to the outer surface of the non-detached rooting device, and the light-shielding layer is made of silver-coated light-shielding cloth.

[0009] Preferably, the rooting capsule placement groove has a semi-circular structure, and the lower end of the rooting capsule placement groove has through holes that are evenly spaced.

[0010] As a preferred option, two branch guide supports are provided, and a rooting zone is provided between the lowest points of the two branch guide supports.

[0011] Preferably, the branch guide holder has an S-shaped structure and is symmetrically arranged about the center line of the main body of the non-in vitro rooting device.

[0012] As a preferred option, a protective pad is fixed to the upper end of the branch guide support, and the protective pad is made of elastic rubber.

[0013] Preferably, the fixing component also includes a U-shaped frame, the front end of the branch guide bracket is fixed with the U-shaped frame, the front end of the U-shaped frame is fixed with an internal threaded sleeve, the inner side of the internal threaded sleeve is connected with a control bolt, and the control bolt is threadedly connected to the internal threaded sleeve.

[0014] The beneficial effects of this utility model are:

[0015] This non-in vitro rooting device for plants, suitable for outdoor use, guides branches to bend naturally and reserves space for rooting through an S-shaped symmetrical branch guide holder. Combined with an elastic rubber protective pad, it significantly reduces epidermal damage. Furthermore, the binding straps and positioning plates allow for adjustable branch tightness to accommodate branches of different thicknesses. A control bolt in the fixing component enables quick locking. Compared to traditional in vitro rooting devices, this device addresses the low survival rate of traditional outdoor rooting techniques while preserving nutrient transfer from the parent plant. It ensures that the branches awaiting rooting can continuously obtain water and nutrients, significantly improving the rooting survival rate, and is particularly suitable for rapidly establishing plant propagation systems in the wild. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of the non-in vitro rooting device of this utility model. Figure 1 ;

[0017] Figure 2 This is a schematic diagram of the overall structure of the non-in vitro rooting device of this utility model. Figure 2 ;

[0018] Figure 3 This is a cross-sectional view of the non-in vitro rooting device of this utility model;

[0019] Figure 4 This is a schematic diagram of the branch guide support structure of this utility model.

[0020] Explanation of reference numerals in the attached diagram: 1. Main body of the non-in vitro rooting device; 2. Support base; 3. Light-shielding layer; 4. Rooting capsule placement groove; 5. Through hole; 6. Support rod; 7. Support bracket; 8. Branch guide bracket; 9. Protective pad; 10. Binding strap; 11. Positioning plate; 12. Fixing component; 13. Rooting area; 1201. U-shaped frame; 1202. Internal threaded sleeve; 1203. Control bolt. Detailed Implementation

[0021] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0022] Existing plant rooting devices are specialized equipment based on plant tissue culture technology, primarily used for detached plant organs. Detached plants refer to a biotechnology system that uses plant tissue culture (PTC) to regenerate detached organs (such as roots, stems, leaves, anthers, etc.) or cells into complete plants in a sterile artificial environment. Its scientific basis stems from the totipotency of plant cells, meaning that a single cell can develop into a complete plant under suitable conditions. Explant selection: Meristems (shoot tips, root tips), leaves, endosperm, pollen, etc., from healthy parent plants are selected as initial materials, requiring high differentiation potential and low pathogen carrying rate. Sterile environment: Microbial contamination is eliminated through physical (autoclaving, ultraviolet irradiation) and chemical (ethanol, sodium hypochlorite) methods to ensure the purity of the culture system. Culture medium control: Based on MS (Murashige and Skoog) medium, the carbon source (30 g / L sucrose), nitrogen, phosphorus and potassium ratio, plant growth regulators (such as 2,4-D to induce callus and NAA to promote rooting) and pH value (5.6-6.0) were adjusted according to the plant species.

[0023] A plant rooting system is a bioengineering device developed based on plant tissue culture (PTC) technology. Its core function is to achieve efficient rooting and regeneration of isolated plant organs under artificially controlled conditions. The in vitro culture system consists of three main elements: explants, culture medium, and an environmental control system.

[0024] Explant selection and treatment: Select meristematic tissues such as shoot tips, leaves, and axillary buds of healthy mother plants as initial materials. After surface sterilization (usually treated with 0.1% HgCl2 or sodium hypochlorite), aseptic inoculation is completed in a clean bench.

[0025] Culture medium optimization: The basal culture medium (such as MS, White, B5 medium) is adjusted according to the plant species to include macroelements (N, P, K), microelements (Fe, Zn, Cu), organic components (vitamins, amino acids) and growth regulators (such as NAA and IBA for root induction, and 6-BA for shoot differentiation).

[0026] Precise environmental control: The integrated equipment simulates the optimal growth conditions for plants, including parameters such as temperature (25±2℃), light intensity (1000-3000 lux), light quality ratio (red light promotes elongation, blue light enhances differentiation), humidity (70-90% RH) and gas exchange (CO2 concentration control).

[0027] Modern plant rooting devices have formed a modular and intelligent technical architecture. Key subsystems include: a controllable environment incubator, which uses a 304 stainless steel inner liner and double-layered insulated glass doors, and is equipped with 4-8 layers of height-adjustable culture racks, each with a load capacity of ≥15kg, adaptable to different sizes of culture containers (such as tissue culture bottles, breathable bags, and aerosol cultivation troughs); a HEPA high-efficiency air filtration system (filtration efficiency ≥99.97%@0.3μm), combined with ultraviolet lamps and an ozone generator to achieve chamber sterilization, controlling the contamination rate to <5%; and a light environment control system, using a full-spectrum LED array, programmably adjustable in the proportions of red light (630-670nm), blue light (430-460nm), and far-red light (730nm), supporting pulsed light modes (such as a 16h / 8h light-dark cycle), and achieving stepless adjustment from 0-5000 lux through PWM dimming technology, with light uniformity ≥85% (based on ISO). (Standard 13690); Equipped with a temperature and humidity coordinated control module, combining semiconductor temperature control chip with PID algorithm, accuracy reaches ±0.5℃, supports day and night temperature difference simulation (e.g., daytime 28℃ / nighttime 22℃), ultrasonic atomizer generates 5-10μm water mist particles, combined with condensate recovery and circulation system, humidity fluctuation range ≤3%, equipped with culture medium dynamic replenishment unit, peristaltic pump injects liquid culture medium containing growth hormone (pH5.8±0.2) according to preset program (e.g., deliver 50mL every 24 hours), conductivity sensor monitors nutrient consumption in real time, culture medium is atomized into 20-50μm aerosol through high pressure nozzle, the root system is exposed to high oxygen environment, nutrient absorption efficiency is improved by more than 40%.

[0028] With the integration of biomanufacturing and IoT technologies, the new generation of devices exhibits significant innovations. By integrating machine vision systems (such as CCD cameras + OpenCV algorithms), it can analyze morphological indicators such as callus formation and root primordia differentiation in real time, automatically adjust the concentration of growth regulators, and has a blockchain traceability system. By putting data on the blockchain of the entire process of tissue culture seedling production (explant source, culture medium formula, environmental parameters), it meets the GACP (Growth Standard for Medicinal Plants) certification requirements. Furthermore, it enables in vitro docking of rootstock and scion within the device, and promotes vascular bundle connection through a microgravity environment (3D rotating support), increasing the grafting survival rate to 92%.

[0029] Plant rooting devices have been widely used in the industrial production of forest tree seedlings, the protection of endangered plants, and the research of transgenic plants. The industrial production of forest tree seedlings is as follows: (1) Eucalyptus rapid propagation: Somatic embryogenesis technology is used, and a single device can produce 500,000 high-quality tissue culture seedlings per year, shortening the rooting cycle to 14 days; (2) Pine mycorrhizal seedling cultivation: Ectomycorrhizal fungi (such as Pisolithus tinctorius) are added to the culture medium to promote the stress resistance of seedlings and increase the survival rate of afforestation by 35%. The protection of endangered plants is as follows: (1) Orchid rescue: Non-symbiotic germination culture of Dendrobium officinale seeds has successfully broken through the technical bottleneck of germination rate <0.1% under natural conditions; (2) Fern rejuvenation: Through in vitro spore culture technology, the seedling survival rate of Alsophila spinulosa spores has been increased from 5% in the wild environment to 78%. Transgenic plant research: (1) Gene editing system: Arabidopsis transformed with CRISPR / Cas9 vectors were screened in the device, and positive seedlings were efficiently sorted by hygromycin resistance markers; (2) Artificial chromosome integration: The hairy root system mediated by Agrobacterium rhizogenes was used to reconstruct the synthetic pathway of medicinal components (such as paclitaxel).

[0030] Please see Figures 1-3This utility model provides an embodiment: a non-in vitro rooting device for plants suitable for outdoor use, comprising a non-in vitro rooting device body 1, a light-shielding layer 3, a rooting capsule placement groove 4, a branch guide support 8, a binding strap 10, and a fixing component 12. A support base 2 is fixed to the lower end of the non-in vitro rooting device body 1. The light-shielding layer 3 is connected to the surface of the non-in vitro rooting device body 1. The rooting capsule placement groove 4 is fixed to the inner wall of the non-in vitro rooting device body 1. A support rod 6 is fixed to the bottom end of the inner wall of the non-in vitro rooting device body 1. A support bracket 7 is fixed to the upper end of the support rod 6. A branch guide support 8 is fixed to the upper end of the non-in vitro rooting device body 1. A protective device is connected to the upper end of the branch guide support 8. The rear end of the pad 9 and the branch guide support 8 is fixed with a tie 10. The front end of the tie 10 is fixed with a positioning 11. The front end of the branch guide support 8 is provided with a fixing group 12. The fixing component 12 includes a control bolt 1203. The support base 2 provides stable support for the main body 1 of the non-detached rooting device. The light-blocking layer 3 can effectively block strong direct sunlight and protect the new roots. The rooting capsule placement groove 4 inside facilitates the placement of fertilizer containing growth hormones or nutrients to promote root development. The branch guide support 8 with its S-shaped symmetrical structure can guide the branches to bend naturally. The fixing component 12 makes it easy to fix the branches in the device to prevent displacement or falling off.

[0031] Please see Figures 2-4In this embodiment, the light-shielding layer 3 is annularly bonded and adhered to the outer surface of the non-detached rooting device body 1. The light-shielding layer 3 is made of silver-coated light-shielding cloth. By using light-shielding material on part of the non-detached rooting device body 1, the damage of strong light to the new root system can be effectively reduced. The rooting capsule placement groove 4 has a semi-circular structure, and the lower end of the rooting capsule placement groove 4 is provided with through holes 5. The through holes 5 are evenly distributed. Multiple through holes 5 facilitate the release of rooting fertilizer in the rooting capsule placement groove 4 into the non-detached rooting device body 1, thereby promoting root development. Two branch guide supports 8 are provided, and a rooting zone 13 is provided between the lowest points of the two branch guide supports 8. The branch guide supports 8 have an S-shaped structure and are symmetrically arranged about the center line of the non-detached rooting device body 1. The S-shaped symmetrical branch guide support 8 can guide the branches to bend naturally so as to achieve non-detached rooting. The upper end of the branch guide support 8 is fixedly connected to a protective pad 9. The protective pad 9 is made of elastic rubber. The elastic rubber protective pad 9 can buffer the friction between the branch and the branch guide support 8, reduce mechanical damage, and prevent epidermal damage. The fixing component 12 also includes a U-shaped frame 1201. The front end of the branch guide support 8 is fixed to the U-shaped frame 1201. The front end of the U-shaped frame 1201 is fixed to an internal threaded sleeve 1202. The inner side of the internal threaded sleeve 1202 is connected to a control screw 1203. The control screw 1203 is threadedly connected to the internal threaded sleeve 1202. By rotating the control screw 1203 in the internal threaded sleeve 1202, the binding strap 10 can be fixed or loosened as needed.

[0032] During the process, a small section of the bark is removed from the target branch. The target branch is then bent into a U-shape along the branch guide support 8, and the section with the bark removed is placed in the rooting area 13. Next, the binding strap 10 is tightened, and then the control bolt 1203 in the internal threaded sleeve 1202 is tightened until the end of the control bolt 1203 abuts against the fixed plate 11. This fixes the target branch to the branch guide support 8 to prevent it from falling off. Finally, water is injected into the main body 1 of the non-detached rooting device. At the same time, the rooting fertilizer in the rooting capsule placement groove 4 is released upon contact with water, thereby promoting root development.

[0033] Through the above steps, the shading layer can effectively block direct sunlight and protect the new roots. The S-shaped symmetrical branch guide can guide the branches to bend naturally. The fixing components can easily fix the branches in the device to prevent displacement or falling off, thus facilitating the branches to achieve non-external rooting, which solves the problem of low survival rate caused by the external rooting method in traditional field rooting devices.

[0034] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention.

Claims

1. A non-in vitro rooting device for plants suitable for outdoor use, comprising a main body (1) of the non-in vitro rooting device; characterized in that: It also includes a light-shielding layer (3), a rooting capsule placement slot (4), a branch guide (8), a binding strap (10), and a fixing component (12). The lower end of the non-in vitro rooting device body (1) is fixed with a support base (2), the surface of the non-in vitro rooting device body (1) is connected with a light-shielding layer (3), the inner wall of the non-in vitro rooting device body (1) is fixed with a rooting capsule placement slot (4), and the bottom end of the inner wall of the non-in vitro rooting device body (1) is fixed with a support rod (6). The upper end of the support rod (6) is fixed with a support bracket (7), the upper end of the non-in vitro rooting device body (1) is fixed with a branch guide bracket (8), the upper end of the branch guide bracket (8) is connected with a protective pad (9), the rear end of the branch guide bracket (8) is fixed with a binding strap (10), the front end of the binding strap (10) is fixed with a positioning piece (11), the front end of the branch guide bracket (8) is provided with a fixing component (12), and the fixing component (12) includes a control bolt (1203).

2. The plant non-in vitro rooting device suitable for outdoor use according to claim 1, characterized in that: The light-shielding layer (3) is a ring-shaped structure bonded to the outer surface of the non-in vitro rooting device body (1), and the light-shielding layer (3) is made of silver-coated light-shielding cloth.

3. The plant non-in vitro rooting device suitable for outdoor use according to claim 1, characterized in that: The rooting capsule placement groove (4) has a semi-circular structure, and the lower end of the rooting capsule placement groove (4) is provided with a through hole (5), which is evenly distributed.

4. The plant non-in vitro rooting device suitable for outdoor use according to claim 1, characterized in that: There are two branch guide supports (8), and a rooting zone (13) is set between the lowest points of the two branch guide supports (8).

5. A non-in vitro rooting device for plants suitable for outdoor use according to claim 4, characterized in that: The branch guide holder (8) has an S-shaped structure and is symmetrically arranged about the center line of the main body (1) of the non-in vitro rooting device.

6. A non-in vitro rooting device for plants suitable for outdoor use according to claim 5, characterized in that: A protective pad (9) is fixed to the upper end of the branch guide support (8), and the protective pad (9) is made of elastic rubber.

7. A non-in vitro rooting device for plants suitable for outdoor use according to claim 1, characterized in that: The fixing component (12) also includes a U-shaped frame (1201), the front end of the branch guide bracket (8) is fixed with the U-shaped frame (1201), the front end of the U-shaped frame (1201) is fixed with an internal threaded sleeve (1202), the inner side of the internal threaded sleeve (1202) is connected with a control bolt (1203), and the control bolt (1203) is threadedly connected to the internal threaded sleeve (1202).

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