Microfluidic detection device and nucleic acid detection equipment

Abstract

The utility model belongs to the technical field of medical detection, and relates to a microfluidic detection device and nucleic acid detection equipment. The microfluidic detection device comprises a base, a detection tube, a reaction tube, a driving rod and a puncturing piece, the base is connected with the detection pipe, and a liquid storage cavity and a detection piece connected with the liquid storage cavity are arranged on the base; a sealing film is arranged at the end, close to the base, of the detection tube, the detection tube is provided with a dilution cavity, a liquid inlet communicated with the dilution cavity is formed in the tube wall of the detection tube, the reaction tube is arranged at the liquid inlet, and a sealing cover is movably arranged at the end, away from the liquid inlet, of the reaction tube; one end of the driving rod penetrates out of the dilution cavity, and the other end of the driving rod is used for sealing the liquid inlet and is arranged in the dilution cavity in a sliding manner; the puncturing piece is connected with the end, close to the base, of the driving rod, and the puncturing piece is used for puncturing the sealing film. According to the invention, portable detection is realized, and the detection efficiency of self-detection is improved.

Description

Technical Field

[0001] This application relates to the field of medical testing technology, and more specifically, to a microfluidic testing device and a nucleic acid testing equipment. Background Technology

[0002] With the improvement of people's living standards and the rapid development of science and technology, people are paying more and more attention to their health. At the same time, with the acceleration of the pace of life, cost, time and privacy have become aspects of concern for people, whether in life or at work. Especially in the field of medical testing, the cumbersome examinations, high testing fees and long waiting times for results in hospitals have caused people trouble. Utility Model Content

[0003] This application provides a microfluidic detection device and a nucleic acid detection equipment to solve the problem of not being able to perform home self-testing in the prior art.

[0004] To address the aforementioned technical problems, this application provides a microfluidic detection device, which employs the following technical solution:

[0005] A microfluidic detection device includes: a base, a detection tube, a reaction tube, a drive rod, and a puncture component; the base is connected to the detection tube, and the base has a liquid storage chamber and a detection component connected to the liquid storage chamber; the detection tube has a sealing membrane at one end near the base, a dilution chamber, and an inlet communicating with the dilution chamber on its wall; the reaction tube is located at the inlet, and a sealing cap is movably provided at one end away from the inlet; one end of the drive rod extends out of the dilution chamber, and the other end of the drive rod is used to seal the inlet and slide within the dilution chamber; the puncture component is connected to the end of the drive rod near the base, and the puncture component is used to puncture the sealing membrane;

[0006] The dilution chamber is used to pre-load the first reaction reagent, and the reaction tube is used to pre-load the second reaction reagent and store the sample. When the drive rod moves away from the base, the seal on the inlet is released, allowing the sample reacting with the second reaction reagent to flow into the dilution chamber, so that the first reaction reagent and the sample are mixed into a mixed liquid. When the drive rod moves closer to the base, the puncturing element punctures the sealing membrane, allowing the mixed liquid to flow into the storage chamber, and the detection element detects the mixed liquid.

[0007] Furthermore, the puncturing component includes a puncture needle and a ring plate. The puncture needle is connected to one end of the drive rod near the base. The ring plate is arranged circumferentially around the puncture needle and is located at one end of the puncture needle near the base.

[0008] Furthermore, a limiting member is provided inside the liquid storage cavity, which is used to abut against the annular plate.

[0009] Furthermore, the distance from the end of the limiting member near the end of the detection tube to the bottom of the liquid storage cavity is greater than the distance from the end of the needle near the base.

[0010] Furthermore, the base is also provided with a channel and a fixing cavity, the channel connecting the fixing cavity and the liquid storage cavity, and the detection element being connected to the fixing cavity.

[0011] Furthermore, the testing component includes a test strip and a fixing clip, the test strip being disposed on the fixing clip, and the end of the fixing clip being connected to the fixing cavity.

[0012] Furthermore, the fixing clip is arranged perpendicularly to the base and parallel to the detection tube.

[0013] Furthermore, the fixing clip includes a body, a first observation window is provided on one side of the body, and an installation groove is provided on the other side of the body along the length direction. A snap-fit ​​block is provided on the groove wall of the installation groove. The test paper is placed in the installation groove and is clamped between the bottom of the installation groove and the snap-fit ​​block. The color development area of ​​the test paper corresponds to the first observation window.

[0014] Furthermore, the microfluidic detection device also includes a housing, which is connected to and parallel to the detection tube, and connected to and perpendicular to the base. The housing covers the outer periphery of the fixing clamp, and the housing has a second observation window that corresponds to the first observation window.

[0015] To address the aforementioned technical problems, this application also provides a nucleic acid detection device, which employs the following technical solution:

[0016] A nucleic acid detection device includes a heating auxiliary device and a microfluidic detection device as described above, wherein the heating auxiliary device is used to heat the reaction tube of the microfluidic detection device.

[0017] Compared with existing technologies, the embodiments of this application have the following main advantages: The reaction tube is used to pre-load the second reaction reagent and store the sample. After sampling, the sample can be directly placed in the reaction tube to carry out the biological reaction, reducing the steps required for operators to obtain the second reaction reagent from the outside during the detection process. Furthermore, the microfluidic detection device does not need to be inverted when placing the sample, thus avoiding leakage of the first reaction reagent. The reaction tube can be sealed or opened via a drive rod, eliminating the need for multiple external pipetting operations during the use of the microfluidic detection device. This avoids the use of complex and bulky instruments, simplifies operation, improves detection efficiency, and enhances flexibility. No professional training or learning is required, and the device can be operated immediately, enabling portable detection and thus improving the efficiency of home-based nucleic acid testing. In addition, the base is connected to the detection tube and a piercing element is set at the end of the drive rod. By moving the drive rod, the piercing element pierces the sealing membrane, allowing the mixed liquid to flow to the detection element. There is no need for the operator to manually align the base and the detection tube, nor is there an additional piercing structure to puncture the sealing membrane. On the one hand, it reduces the number of steps for the operator, on the other hand, it can reduce sample contamination and also avoid the operator being accidentally injured by the additional piercing structure. Attached Figure Description

[0018] To more clearly illustrate the solution of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the structure of a microfluidic detection device provided in an embodiment of this application;

[0020] Figure 2 yes Figure 1 A sectional view;

[0021] Figure 3 yes Figure 1 Exploded view;

[0022] Figure 4 yes Figure 1 A schematic diagram of the structure after concealing the outer casing and detection tube;

[0023] Figure 5 yes Figure 4 A structural schematic diagram of the central base and the testing component from another perspective.

[0024] Reference numerals: 10, base; 11, storage chamber; 12, limiting element; 13, passageway; 14, fixing chamber; 20, detection tube; 21, sealing membrane; 22, dilution chamber; 23, inlet; 30, reaction tube; 31, sealing cap; 40, drive rod; 41, push rod; 42, piston; 50, puncture element; 51, puncture needle; 52, ring plate; 60, detection element; 61, test paper; 62, fixing clamp; 621, body; 622, mounting groove; 623, snap-fit ​​block; 70, outer shell. Detailed Implementation

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used herein in the specification of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "comprising" and "having," and any variations thereof, in the specification, claims, and foregoing drawings of this application are intended to cover non-exclusive inclusion. The terms "first," "second," etc., in the specification, claims, or foregoing drawings of this application are used to distinguish different objects, not to describe a particular order.

[0026] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0027] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings.

[0028] This application provides a microfluidic detection device, such as... Figures 1 to 5As shown, the microfluidic detection device includes: a base 10, a detection tube 20, a reaction tube 30, a drive rod 40, and a puncture element 50; the base 10 is connected to the detection tube 20, and the base 10 is provided with a liquid storage chamber 11 and a detection element 60 connected to the liquid storage chamber 11; the detection tube 20 is provided with a sealing membrane 21 at one end near the base 10, the detection tube 20 has a dilution chamber 22, and the tube wall of the detection tube 20 has an inlet 23 communicating with the dilution chamber 22; the reaction tube 30 is located at the inlet 23, and a sealing cap 31 is movably provided at one end of the reaction tube 30 away from the inlet 23; one end of the drive rod 40 extends out of the dilution chamber 22, and the other end of the drive rod 40 is used to seal the inlet 23 and slide within the dilution chamber 22; the puncture element 50 is connected to the end of the drive rod 40 near the base 10, and the puncture element 50 is used to puncture the sealing membrane 21;

[0029] The dilution chamber 22 is used to pre-load the first reaction reagent, and the reaction tube 30 is used to pre-load the second reaction reagent and store the sample. When the drive rod 40 moves away from the base 10, the seal on the liquid inlet 23 is released, allowing the sample after reacting with the second reaction reagent to flow into the dilution chamber 22, so that the first reaction reagent and the sample are mixed into a mixed liquid. When the drive rod 40 moves closer to the base 10, the puncturing element 50 punctures the sealing membrane 21, allowing the mixed liquid to flow into the storage chamber 11, and the detection element 60 detects the mixed liquid.

[0030] The working principle of the microfluidic detection device provided in this application embodiment is as follows: First, a quantitative amount of first reaction reagent is pre-loaded in the dilution chamber 22, and a quantitative amount of second reaction reagent is pre-loaded in the reaction tube 30; during sampling, the base 10 is placed on a flat surface, and the sealing cap 31 is removed from the reaction tube 30 to remove the seal on the reaction chamber; after sampling, the lysed sample is placed in the reaction tube 30, and then the sealing cap 31 is immediately put back on the reaction tube 30 to seal the reaction chamber, allowing the second reaction reagent to undergo a biological reaction with the sample; then, the drive rod 40 is moved away from the base 10, causing the drive rod 40 to remove... The seal between the inlet 23 and the liquid inlet is broken. At this time, a negative pressure is generated in the detection tube 20. Under the action of the negative pressure, the sample that has reacted with the second reaction reagent in the reaction chamber flows into the dilution chamber 22, so that the first reaction reagent and the sample are mixed. Then, the detection tube 20 is inverted several times to make the first reaction reagent and the sample fully mixed to obtain a mixed liquid. Finally, the drive rod 40 is moved towards the base 10. The drive rod 40 drives the puncturing element 50 to puncture the sealing film 21, so that the mixed liquid flows into the storage chamber 11. Then the mixed liquid flows to the detection element 60, which detects the mixed liquid and displays the detection result.

[0031] The beneficial effects of the microfluidic detection device provided in this application embodiment are as follows: The reaction tube 30 is used to pre-load the second reaction reagent and store the sample. After sampling, the sample can be directly placed in the reaction tube 30 to carry out the biological reaction, reducing the step of requiring the operator to obtain the second reaction reagent from the outside to carry out the biological reaction with the sample during the detection process. Moreover, the microfluidic detection device does not need to be inverted when placing the sample, which can avoid leakage of the first reaction reagent. The reaction tube 30 can be sealed or opened by the drive rod 40, so that during the use of the microfluidic detection device, there is no need to perform multiple external pipetting operations, avoiding the use of complex and bulky instruments and equipment. The reduction of external pipetting operations simplifies the operation, improves detection efficiency, and enhances flexibility. No professional training or learning is required, and it can be operated by hand, realizing portable detection, thereby improving the detection efficiency of nucleic acid home self-testing. In addition, the base 10 is connected to the detection tube 20, and a piercing element 50 is provided at the end of the drive rod 40. By moving the drive rod 40, the piercing element 50 pierces the sealing membrane 21, allowing the mixed liquid to flow to the detection element 60. There is no need for the operator to manually align the base 10 with the detection tube 20, nor is there a need for an additional piercing structure to pierce the sealing membrane 21. On the one hand, this reduces the number of steps for the operator, on the other hand, it can reduce sample contamination, and it can also prevent the operator from being accidentally injured by an additional piercing structure.

[0032] Furthermore, the first reaction reagent is a biological reaction reagent, such as a diluent, which is used to dilute the second reaction reagent and the reaction reagent after the viral nucleic acid reaction.

[0033] Furthermore, the second reaction reagent is a biological reaction reagent, such as a lyophilized reagent, which can be in powder or solid sphere form, and is used to amplify the lysed sample.

[0034] Furthermore, the sealing film 21 is an aluminum film, which is welded to the bottom of the detection tube 20 by heat sealing or welding to seal the first reaction reagent pre-loaded inside the detection tube 20.

[0035] Specifically, the aluminum film can be heat-sealed by heating it at 160-180°C for 2-5 seconds using a heat sealer. After heat sealing, the aluminum film has super strong adhesion and sealing properties, which can reduce the evaporation of the first reaction reagent. The aluminum film is relatively thin and can be punctured under force. In addition, the aluminum film has good biocompatibility and will not react with the first reaction reagent.

[0036] Furthermore, each of the two sides of the detection tube 20 is provided with an inlet 23, and each inlet 23 is provided with a reaction tube 30.

[0037] In this embodiment, two reaction tubes 30 can be used to place two different second reaction reagents, or different amounts of the same second reaction reagent can be placed.

[0038] Specifically, the reaction tube 30 and the detection tube 20 are integrally injection molded.

[0039] Specifically, the sealing cap 31 can be snapped or threaded onto the reaction tube 30.

[0040] Specifically, the sealing cap 31 can be made of plastic or silicone.

[0041] Furthermore, the base 10 and the detection tube 20 are fixed by laser welding or ultrasonic welding.

[0042] like Figures 2 to 4 As shown, the puncture member 50 further includes a needle 51 and a ring plate 52. The needle 51 is connected to one end of the drive rod 40 near the base 10. The ring plate 52 is arranged around the circumference of the needle 51 and is located at one end of the needle 51 near the base 10.

[0043] In this embodiment, when the drive rod 40 is moved toward the base 10, the drive rod 40 drives the needle 51 to puncture the sealing membrane 21. As the drive rod 40 moves, the ring plate 52 pushes open the sealing membrane 21, allowing the mixed liquid to flow into the storage chamber 11. Then the mixed liquid flows to the detection element 60, which detects the mixed liquid and displays the detection result.

[0044] In this embodiment, a ring plate 52 is provided around the needle 51 to prevent the sealing membrane 21 from sticking to the needle 51 after the needle 51 punctures the sealing membrane 21, thereby allowing the mixed liquid to flow smoothly into the storage chamber 11.

[0045] like Figure 4 , 5 As shown, the liquid storage cavity 11 is further provided with a limiting member 12, which is used to abut against the ring piece 52.

[0046] In this embodiment, when the drive rod 40 is moved toward the base 10, the drive rod 40 drives the needle 51 to puncture the sealing membrane 21. As the drive rod 40 moves, the ring plate 52 pushes open the sealing membrane 21, and then the ring plate 52 abuts against the limiting member 12. The mixed liquid flows into the storage chamber 11 and then flows to the detection member 60. The detection member 60 detects the mixed liquid and displays the detection result.

[0047] In this embodiment, by abutting the limiting member 12 against the ring plate 52, the movement of the drive rod 40 and the ring plate 52 is limited, which can realize the quantitative addition of liquid to the liquid storage chamber 11; and the abutting of the limiting member 12 against the ring plate 52 can bring resistance to prompt the operator to move the drive rod 40 into place, so that the operator does not need to observe the movement of the drive rod 40, reducing the difficulty of operation.

[0048] Furthermore, the distance from the end of the limiting member 12 near the end of the detection tube 20 to the bottom of the liquid storage chamber 11 is greater than the distance from the end of the needle 51 near the end of the base 10 to the ring plate 52.

[0049] In this embodiment, the relationship between the distance from the end of the limiting member 12 to the bottom of the liquid storage cavity 11 and the distance from the end of the needle 51 to the ring plate 52 is limited to prevent the end of the needle 51 from damaging the bottom of the liquid storage cavity 11 of the base 10 when the ring plate 52 abuts against the limiting member 12, thus preventing leakage from the base 10.

[0050] Specifically, the limiting member 12 can be sheet-shaped, extending from the bottom of the liquid storage cavity 11 toward the detection tube 20; the limiting member 12 can also be block-shaped, disposed on the cavity wall of the liquid storage cavity 11.

[0051] Furthermore, the base 10 is also provided with a channel 13 and a fixing cavity 14. The channel 13 connects the fixing cavity 14 and the liquid storage cavity 11, and the detection element 60 is connected to the fixing cavity 14.

[0052] In this embodiment, the drive rod 40 is moved closer to the base 10, and the drive rod 40 drives the puncturing member 50 to puncture the sealing film 21, so that the mixed liquid flows into the storage chamber 11, then the mixed liquid flows into the channel 13, and finally the mixed liquid flows into the fixed chamber 14. The detection member 60 detects the mixed liquid and displays the detection result.

[0053] In this embodiment, the fixed cavity 14 and the liquid storage cavity 11 are connected by the channel 13, so that the flow rate of the mixed liquid can be controlled by the channel 13. This can prevent a large amount of mixed liquid from flowing directly to the detection element 60, prevent the detection element 60 from being quickly wetted, and improve the detection accuracy of the detection element 60.

[0054] like Figure 4 , 5 As shown, the detection element 60 further includes a test strip 61 and a fixing clip 62. The test strip 61 is disposed on the fixing clip 62, and the end of the fixing clip 62 is connected to the fixing cavity 14.

[0055] In this embodiment, after the mixed liquid flows into the fixed chamber 14, the mixed liquid slowly precipitates into the test paper 61, and finally the biochemical reaction results are viewed through the test paper 61.

[0056] In this embodiment, a fixing clip 62 is used to fix the test strip 61 to prevent the test strip 61 from shifting.

[0057] Furthermore, the fixing clip 62 is arranged perpendicularly to the base 10, and the fixing clip 62 is arranged parallel to the detection tube 20.

[0058] In this embodiment, the fixing clip 62 is set perpendicular to the base 10. On the one hand, this can reduce the volume of the microfluidic detection device, and on the other hand, it can further control the speed at which the test strip 61 absorbs the mixed liquid, thus avoiding the problem of detection failure caused by the test strip head absorbing too much water.

[0059] Furthermore, the fixing clip 62 includes a body 621, a first observation window (not shown) is provided on one side of the body 621, and a mounting groove 622 is provided on the other side of the body 621 along the length direction. A snap-fit ​​block 623 is provided on the groove wall of the mounting groove 622. The test paper 61 is disposed in the mounting groove 622 and is clamped between the bottom of the mounting groove 622 and the snap-fit ​​block 623. The color development area of ​​the test paper 61 corresponds to the first observation window.

[0060] In this embodiment, when the mixed liquid flows to the test strip 61, the test result can be observed through the first observation window. After the reaction is completed, the test strip 61 will change color, and the test result can be judged as negative or positive by the color change. The test strip 61 is clamped between the bottom of the mounting groove 622 and the snap-fit ​​block 623 to fix the test strip 61.

[0061] Furthermore, the microfluidic detection device also includes a housing 70, which is connected to and parallel to the detection tube 20, and connected to and perpendicular to the base 10. The housing 70 covers the outer periphery of the fixing clamp 62, and the housing 70 has a second observation window (not shown), which corresponds to the first observation window.

[0062] In this embodiment, when the mixed liquid flows to the test strip 61, the test result can be observed through the second observation window and the first observation window. After the reaction is completed, the test strip 61 will change color, and the test result can be judged as negative or positive by the color change.

[0063] In this embodiment, a housing 70 is provided around the outer periphery of the fixing clamp 62, so that aerosols will not leak out through the detection element 60 during the detection reaction, thus avoiding aerosol contamination. At the same time, the sealing of the microfluidic detection device can reduce interference from external factors, avoid contamination of the sample and the test strip 61, and thus improve the detection accuracy.

[0064] Furthermore, the outer shell 70 is also covered over the passageway 13 to further prevent aerosol pollution.

[0065] Furthermore, the base 10 and the outer shell 70 are fixed by laser welding or ultrasonic welding.

[0066] Furthermore, the drive rod 40 includes a push rod 41 and a piston 42. One end of the push rod 41 extends out of the dilution chamber 22, and the other end of the push rod 41 is connected to the piston 42. The piston 42 is used to seal the liquid inlet 23 and slides within the dilution chamber 22.

[0067] Furthermore, the piston 42 is press-fitted with the inner wall of the detection tube 20 to prevent leakage of the second reaction reagent pre-loaded in the reaction tube 30.

[0068] This application embodiment also provides a nucleic acid detection device, which includes a heating auxiliary device and a microfluidic detection device as described above. The heating auxiliary device is used to heat the reaction tube 30 of the microfluidic detection device.

[0069] The working principle of the nucleic acid detection device provided in this application embodiment is as follows: First, a quantitative amount of first reaction reagent is pre-filled in the dilution chamber 22, and a quantitative amount of second reaction reagent is pre-filled in the reaction tube 30; during sampling, the base 10 is placed on a flat surface, and the sealing cap 31 is removed from the reaction tube 30 to remove the seal on the reaction chamber; after sampling, the lysed sample is placed in the reaction tube 30, and then the sealing cap 31 is immediately put back on the reaction tube 30 to seal the reaction chamber, and the reaction tube 30 is heated by a heating sealing auxiliary device to increase the biological reaction rate between the sample and the second reaction reagent; then, the drive rod 40 is moved away from the base 10. The drive rod 40 is moved to release the seal between itself and the inlet 23. At this time, a negative pressure is generated in the detection tube 20. Under the action of the negative pressure, the sample that has reacted with the second reaction reagent in the reaction chamber flows into the dilution chamber 22, so that the first reaction reagent and the sample are mixed. Then, the detection tube 20 is inverted several times to ensure that the first reaction reagent and the sample are fully mixed to obtain a mixed liquid. Finally, the drive rod 40 is moved closer to the base 10, and the drive rod 40 drives the puncturing element 50 to puncture the sealing membrane 21, so that the mixed liquid flows into the storage chamber 11. Then the mixed liquid flows to the detection element 60, which detects the mixed liquid and displays the detection result.

[0070] The beneficial effects of the nucleic acid detection device provided in this application embodiment are as follows: The reaction tube 30 is used to pre-load the second reaction reagent and store the sample. After sampling, the sample can be directly placed in the reaction tube 30 to carry out the biological reaction. This reduces the step of requiring the operator to obtain the second reaction reagent from the outside to carry out the biological reaction with the sample during the detection process. Moreover, the microfluidic detection device does not need to be inverted when placing the sample, which can avoid leakage of the first reaction reagent. The reaction tube 30 can be sealed or opened by the drive rod 40, so that during the use of the microfluidic detection device, there is no need to perform multiple external pipetting operations. This avoids the use of complex and bulky instruments and equipment, reduces external pipetting operations, simplifies operation, improves detection efficiency, and increases flexibility. No professional training or learning is required, and it can be operated by hand, realizing portable detection, thereby improving the detection efficiency of nucleic acid home self-testing. In addition, the base 10 is connected to the detection tube 20, and a piercing element 50 is provided at the end of the drive rod 40. By moving the drive rod 40, the piercing element 50 pierces the sealing membrane 21, allowing the mixed liquid to flow to the detection element 60. There is no need for the operator to manually align the base 10 with the detection tube 20, nor is there a need for an additional piercing structure to pierce the sealing membrane 21. On the one hand, this reduces the number of steps for the operator, on the other hand, it can reduce sample contamination, and it can also prevent the operator from being accidentally injured by an additional piercing structure.

[0071] Specifically, the heating auxiliary device heats and adjusts the ambient temperature of the environment where the microfluidic detection device is placed to 42°C or 65°C to promote the reaction between the second reaction reagent and the sample.

[0072] Obviously, the embodiments described above are only some embodiments of this application, not all embodiments. The accompanying drawings show preferred embodiments of this application, but do not limit the patent scope of this application. This application can be implemented in many different forms; rather, the purpose of providing these embodiments is to provide a more thorough and comprehensive understanding of the disclosure of this application. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing specific embodiments, or make equivalent substitutions for some of the technical features. Any equivalent structures made using the content of this application's specification and drawings, directly or indirectly applied to other related technical fields, are similarly within the scope of patent protection of this application.

Claims

1. A microfluidic detection device, characterized in that, include: The device comprises a base, a detection tube, a reaction tube, a drive rod, and a piercing element. The base is connected to the detection tube and has a liquid storage chamber and a detection element connected to the liquid storage chamber. The detection tube has a sealing membrane at one end near the base and a dilution chamber. An inlet communicating with the dilution chamber is opened in the tube wall of the detection tube. The reaction tube is located at the inlet, and a sealing cap is movably provided at the end of the reaction tube away from the inlet. One end of the drive rod extends out of the dilution chamber, and the other end of the drive rod is used to seal the inlet and slide within the dilution chamber. The piercing element is connected to the end of the drive rod near the base and is used to pierce the sealing membrane. The dilution chamber is used to pre-load the first reaction reagent, and the reaction tube is used to pre-load the second reaction reagent and store the sample. When the drive rod moves away from the base, the seal on the inlet is released, allowing the sample reacting with the second reaction reagent to flow into the dilution chamber, so that the first reaction reagent and the sample are mixed into a mixed liquid. When the drive rod moves closer to the base, the puncturing element punctures the sealing membrane, allowing the mixed liquid to flow into the storage chamber, and the detection element detects the mixed liquid.

2. The microfluidic detection device according to claim 1, characterized in that, The puncturing component includes a puncture needle and a ring plate. The puncture needle is connected to one end of the drive rod near the base. The ring plate is arranged circumferentially around the puncture needle and is located at one end of the puncture needle near the base.

3. The microfluidic detection device according to claim 2, characterized in that, The liquid storage cavity is provided with a limiting member, which is used to abut against the ring plate.

4. The microfluidic detection device according to claim 3, characterized in that, The distance from the end of the limiting member near the end of the detection tube to the bottom of the liquid storage cavity is greater than the distance from the end of the needle near the base.

5. The microfluidic detection device according to any one of claims 1 to 4, characterized in that, The base is also provided with a channel and a fixed cavity. The channel connects the fixed cavity and the liquid storage cavity, and the detection element is connected to the fixed cavity.

6. The microfluidic detection device according to claim 5, characterized in that, The testing component includes a test strip and a fixing clip. The test strip is disposed on the fixing clip, and the end of the fixing clip is connected to the fixing cavity.

7. The microfluidic detection device according to claim 6, characterized in that, The fixing clamp is arranged perpendicularly to the base and parallel to the detection tube.

8. The microfluidic detection device according to claim 6, characterized in that, The fixing clip includes a body, a first observation window on one side of the body, and an installation groove on the other side of the body along the length direction. A snap-fit ​​block is provided on the groove wall of the installation groove. The test paper is placed in the installation groove and is held between the bottom of the installation groove and the snap-fit ​​block. The color development area of ​​the test paper corresponds to the first observation window.

9. The microfluidic detection device according to claim 8, characterized in that, The microfluidic detection device also includes a housing, which is connected to and parallel to the detection tube, and connected to and perpendicular to the base. The housing covers the outer periphery of the fixing clamp, and the housing has a second observation window that corresponds to the first observation window.

10. A nucleic acid detection device, characterized in that, It includes a heating auxiliary device and a microfluidic detection device as described in any one of claims 1 to 9, wherein the heating auxiliary device is used to heat the reaction tube of the microfluidic detection device.

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