Microbial fermentation seed transferring device

Abstract

The utility model relates to a microbial fermentation seed transferring device which comprises a seed tank, a fermentation tank; a main pipeline; a seed tank first air pipe; a seed tank second air pipe; a fermentation tank first air pipe; a fermentation tank second air pipe; before seed transferring, the first air pipe of the seed tank conveys air to the seed tank, and the first air pipe of the fermentation tank conveys air to the fermentation tank; in the seed transferring process, the second air pipe of the seed tank conveys air to the seed tank, the second air pipe of the fermentation tank conveys air to the fermentation tank, and seed liquid in the seed tank is conveyed to the fermentation tank through the main pipeline. According to the microbial fermentation seed transferring device disclosed by the utility model, the pipeline design of the microbial fermentation seed transferring device is optimized, so that the seed liquid is prevented from oxygen deficit in the main pipeline in the seed transferring process of the seed tank, the bubbling phenomenon generated in the seed transferring process of the fermentation tank can be avoided, and the reverse transmission can be started under the condition that the seed liquid is relatively thick; seeds are transplanted after the seed solution is diluted, so that the performance is more perfect, and the application is wider.

Description

Technical Field

[0001] This utility model relates to the field of microbial transfer device technology, and in particular to a microbial fermentation transfer device. Background Technology

[0002] The microbial fermentation transfer device mainly includes a seed tank, a fermenter, a sterile air supply system, a transfer pipe, a seed tank air pipe, and a fermenter air pipe. The seed tank and the fermenter are each equipped with an electric mixer. One end of the transfer pipe is connected to the discharge port at the bottom of the seed tank, and the other end is connected to the feed port at the top or upper middle part of the fermenter. The seed tank air pipe is inserted from the top of the seed tank and extends into the culture medium at the bottom of the seed tank. The fermenter air pipe is inserted from the top of the fermenter and extends into the culture medium at the bottom of the fermenter.

[0003] When the microbial fermentation transfer device is in operation, firstly, the microbial inoculum in the seed tank matures into a certain number of mycelia and inoculum under the continuous supply of sterile air and the specified fermentation process conditions, thus obtaining seed liquid. Then, the seed liquid is transported to the fermentation tank through the transfer pipeline, realizing the transfer of mature inoculum from the seed tank to the fermentation tank. This transfer process is called "transfer". Then, the seed liquid is further fermented in the fermentation tank.

[0004] During transplanting, the pressure difference between the seed tank and the fermentation tank is adjusted. Under the action of air pressure, the seed liquid in the seed tank is transported to the fermentation tank. Specifically, first, the sterile air outlet valve of the seed tank is closed, and the pressure inside the seed tank is increased. The pressure gauge shows that the tank pressure of the seed tank is 1-2 kg / cm³. 2 Under this pressurized condition, the seed liquid enters the transfer pipe from the bottom valve of the seed tank. Simultaneously, by adjusting the sterile air intake and exhaust valves of the fermenter, the tank pressure is maintained at 0.2-0.6 kg / cm². 2 A pressure difference is created between the seed tank and the fermentation tank, and the seed culture is transferred into the fermentation tank through the transfer pipe. During the transfer process, it is usually necessary to repeatedly adjust the sterile air intake and exhaust of the fermentation tank, especially for large fermentation tanks, where the sterile air intake and exhaust need to be adjusted even more frequently. Since the air pipe of the fermentation tank extends into the culture medium, repeated adjustments to the intake and exhaust can easily cause foaming of the sterile culture medium in the fermentation tank, abnormally raising the fermentation broth level, and posing a risk of overflowing or overfilling, ultimately prolonging the transfer time. Utility Model Content

[0005] Based on the aforementioned deficiencies in the existing technology, the purpose of this utility model is to provide a microbial fermentation transfer device. The pipeline design of the microbial fermentation transfer device is optimized to avoid oxygen deficiency in the main pipeline during the transfer process in the seed tank, and to avoid foaming during the fermentation process in the fermenter. It can also enable reverse transfer when the seed liquid is relatively viscous to dilute the seed liquid before transfer, resulting in more complete performance and wider application.

[0006] Therefore, the present invention provides the following technical solution.

[0007] This utility model provides a microbial fermentation transfer device, the microbial fermentation transfer device comprising:

[0008] Seed container, used to cultivate microbial strains;

[0009] Fermentation tank, used to ferment seed liquid;

[0010] The main pipe has its feed end inserted from the top of the seed tank and extends to the bottom of the seed tank, and its discharge end inserted from the top of the fermentation tank and extends to the bottom of the fermentation tank.

[0011] The first air pipe of the seed tank has its outlet end connected to the main pipe;

[0012] The second air tube of the seed tank has its outlet end inserted from the top of the seed tank and located in the upper part of the seed tank;

[0013] The first air pipe of the fermenter has its outlet end connected to the main pipe;

[0014] The second air pipe of the fermenter has its outlet end inserted from the top of the fermenter and located in the upper part of the fermenter;

[0015] Before transplanting, the first air pipe of the seed tank supplies air to the seed tank, and the first air pipe of the fermentation tank supplies air to the fermentation tank;

[0016] During the transplanting process, the second air pipe of the seed tank supplies air to the seed tank, the second air pipe of the fermentation tank supplies air to the fermentation tank, and the seed liquid in the seed tank is transported to the fermentation tank through the main pipe.

[0017] Optionally, the microbial fermentation transfer device further includes a steam input pipe, the outlet of which is connected to the main pipeline.

[0018] Optionally, the inlet end of the second air pipe of the seed tank is connected to the first air pipe of the seed tank.

[0019] Optionally, the first air pipe of the seed tank is provided with a first control valve and a second control valve, and the second air pipe of the seed tank is provided with a third control valve; the first control valve is located between the inlet end of the first air pipe of the seed tank and the inlet end of the second air pipe of the seed tank, and the second control valve is located between the inlet end of the second air pipe of the seed tank and the outlet end of the first air pipe of the seed tank.

[0020] Optionally, the inlet end of the second air pipe of the fermenter is connected to the first air pipe of the fermenter.

[0021] Optionally, the first air pipe of the fermenter is provided with a fourth control valve and a fifth control valve, and the second air pipe of the fermenter is provided with a sixth control valve; the fourth control valve is located between the inlet end of the first air pipe of the fermenter and the inlet end of the second air pipe of the fermenter, and the fifth control valve is located between the inlet end of the second air pipe of the fermenter and the outlet end of the first air pipe of the fermenter.

[0022] Optionally, the outlet end of the steam input pipe is located between the outlet end of the first air pipe of the seed tank and the outlet end of the first air pipe of the fermenter;

[0023] The main pipeline is also equipped with a seventh control valve and an eighth control valve. The seventh control valve is located between the outlet end of the steam input pipe and the outlet end of the first air pipe of the seed tank, and the eighth control valve is located between the outlet end of the steam input pipe and the outlet end of the first air pipe of the fermenter.

[0024] Optionally, a ninth control valve is provided on the steam input pipe.

[0025] Optionally, the top of the seed tank is connected to a first exhaust pipe, and the first exhaust pipe is equipped with a tenth control valve; the top of the fermentation tank is connected to a second exhaust pipe, and the second exhaust pipe is equipped with an eleventh control valve.

[0026] Optionally, the main pipeline is provided with multiple discharge ends, each discharge end is matched with a fermentation tank, and each fermentation tank is equipped with a first air pipe and a second air pipe.

[0027] This utility model has the following technical effects:

[0028] This invention provides a microbial fermentation transfer device, which optimizes the piping design by adding a second air pipe to the seed tank and a second air pipe to the fermentation tank, and connecting the first air pipes of the seed tank and the fermentation tank to the main pipeline. During the transfer process in the seed tank, the first air pipe of the seed tank supplies a small amount of air to the main pipeline, preventing oxygen deficiency in the seed solution within the main pipeline. During the transfer process in the fermentation tank, it avoids or reduces foaming of the culture medium caused by air supply from the fermentation tank. Furthermore, when the seed solution is viscous, it can be transferred in reverse, transferring the culture medium from the fermentation tank to the seed tank to dilute the seed solution before transfer, resulting in improved performance and wider application. Attached Figure Description

[0029] Figure 1 This is a schematic diagram of the microbial fermentation transfer device of this utility model.

[0030] Explanation of reference numerals in the attached figures

[0031] 100. Microbial fermentation transfer device;

[0032] 1. Seed tank; 2. Fermentation tank; 3. Main pipeline; 31. Feed end; 32. Discharge end; 41. First air pipe of seed tank; 42. Second air pipe of seed tank; 51. First air pipe of fermentation tank; 52. Second air pipe of fermentation tank; 6. Steam input pipe; 71. First control valve; 72. Second control valve; 73. Third control valve; 74. Fourth control valve; 75. Fifth control valve; 76. Sixth control valve; 77. Seventh control valve; 78. Eighth control valve; 79. Ninth control valve; 7010. Tenth control valve; 7011. Eleventh control valve; 7021. First exhaust valve; 7022. Second exhaust valve; 7023. Third exhaust valve; 7024. Fourth exhaust valve; 7025. Fifth exhaust valve; 7026. Sixth exhaust valve; 8. First exhaust pipe; 9. Second exhaust pipe. Detailed Implementation

[0033] To make the technical solution and beneficial effects of this utility model more apparent and understandable, a detailed description is provided below by listing specific embodiments. Unless otherwise defined, the technical and scientific terms used herein have the same meanings as those in the technical field to which this application pertains.

[0034] In the description of this utility model, unless otherwise expressly defined, the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "height", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the purpose of simplifying the description of this utility model and do not indicate that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. That is, they should not be construed as limitations on this utility model.

[0035] In this utility model, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating the relative importance of the indicated features or the number of indicated technical features. Therefore, a feature specified as "first" or "second" can explicitly include at least one of that feature. In the description of this utility model, "a plurality of" means at least two; "several" means at least one; unless otherwise expressly defined.

[0036] In this utility model, unless otherwise explicitly defined, the terms "installation," "connection," "linking," "fixing," and "setting," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral molding; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can also refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0037] In this utility model, unless otherwise explicitly defined, the terms "above," "on top of," "above," "over," "below," "below," "below," or "below" for "first feature above second feature" can refer to direct contact between the first and second features, or indirect contact between the first and second features through an intermediate medium. Furthermore, "above," "above," and "over" for "first feature above second feature" can mean the first feature is directly above or diagonally above the second feature, or simply indicates that the horizontal height of the first feature is higher than the horizontal height of the second feature. Similarly, "below," "below," and "below" for "first feature below second feature" can mean the first feature is directly below or diagonally below the second feature, or simply indicates that the horizontal height of the first feature is lower than the horizontal height of the second feature.

[0038] The following is based on Figure 1 This invention provides a detailed description of the microbial fermentation transfer device.

[0039] In this embodiment, such as Figure 1As shown, the microbial fermentation transfer device 100 includes a seed tank 1, a fermentation tank 2, a main pipe 3, a first air pipe 41 for the seed tank, a second air pipe 42 for the seed tank, a first air pipe 51 for the fermentation tank, and a second air pipe 52 for the fermentation tank. The seed tank 1 contains a culture medium for cultivating microbial inoculum, while the fermentation tank 2 contains a culture medium for fermentation to ferment the seed liquid. The inlet end 31 of the main pipe 3 is inserted from the top of the seed tank 1 and extends to the bottom of the seed tank 1. The outlet end 32 of the main pipe 3 is inserted from the top of the fermentation tank 2 and extends to the bottom of the fermentation tank 2. After the microbial inoculum matures into a seed liquid in the seed tank 1, the seed liquid enters the main pipe 3 through the inlet end 31, and is then transported through the main pipe 3 to the outlet end 32, and finally delivered to the fermentation tank 2.

[0040] The outlet end of the first air pipe 41 of the seed tank is connected to the main pipe 3. The outlet end of the second air pipe 42 of the seed tank is inserted from the upper part of the seed tank 1 and located in the upper part of the seed tank 1. The outlet end of the first air pipe 51 of the fermentation tank is connected to the main pipe 3. The outlet end of the second air pipe 52 of the fermentation tank is inserted from the upper part of the fermentation tank 2 and located in the upper part of the fermentation tank 2. The outlet end of the second air pipe 42 of the seed tank can be inserted from the upper part of one side wall of the seed tank 1 or from the top wall of the seed tank 1. Similarly, the outlet end of the second air pipe 52 of the fermentation tank can be inserted from the upper part of one side wall of the fermentation tank 2 or from the top wall of the fermentation tank 2.

[0041] Specifically, the working steps of seed tank 1 include:

[0042] (1) Sterilize seed tank 1;

[0043] (2) Ventilation: After sterilization, the seed tank 1 needs to be ventilated and pressurized. Specifically, the first air pipe 41 of the seed tank delivers air to the main pipe 3. The air is delivered to the bottom of the seed tank 1 through the main pipe 3 and directly delivered to the culture medium in the seed tank 1, which is conducive to the air being more evenly dispersed in the culture medium.

[0044] (3) Inoculation and cultivation: The seed tank 1 is inoculated with microbial strains using the differential pressure method or the fire ring protection method. The required amount of air is continuously supplied to the seed tank 1 through the first air pipe 41 of the seed tank. The microbial strains grow and mature into a certain number of mycelia and bacterial counts to obtain seed liquid.

[0045] (4) Transplanting: Open the main pipe 3 and reduce the amount of air supplied by the first air pipe 41 of the seed tank. Since the first air pipe 41 of the seed tank is connected to the main pipe 3, it can supply a small amount of air to the main pipe 3. The second air pipe 42 of the seed tank supplies a larger amount of air to the seed tank 1 to increase the pressure in the seed tank 1. Then, under the pressure of the seed tank 1, the seed liquid enters the main pipe 3. Furthermore, since the first air pipe 41 of the seed tank supplies a small amount of air to the main pipe 3, it can provide oxygen to the seed liquid in the main pipe 3, preventing oxygen deficiency in the seed liquid in the main pipe 3. It should be explained that during the transplanting process, the small amount of air supplied by the first air pipe 41 of the seed tank will not affect the entry of the seed liquid into the main pipe 3, that is, it will not interfere with the transplanting.

[0046] The working steps of fermenter 2 include:

[0047] (1) Sterilize fermenter 2;

[0048] (2) Ventilation: After sterilization, the fermenter 2 needs to be ventilated and pressurized. Specifically, air is supplied to the fermenter 2 through the first air pipe 51 of the fermenter and delivered to the bottom of the fermenter 2 through the main pipe 3. The air is directly delivered to the culture medium of the fermenter 2, which is conducive to the air being more evenly dispersed in the culture medium.

[0049] (3) Transplanting: The first air pipe 51 of the fermenter stops supplying air to the fermenter 2, and the second air pipe 52 of the fermenter supplies air to the fermenter 2. By adjusting the supply volume, the tank pressure is reduced, so that the seed liquid in the main pipe 3 enters the fermenter 2. In addition, the second air pipe 52 of the fermenter directly supplies air to the top of the culture medium, which can avoid or reduce the foaming phenomenon of the culture medium caused by air supply (the culture medium is rich in organic nitrogen sources and usually contains foaming substances such as peptone and corn steep liquor. Therefore, repeated air supply can easily lead to foaming).

[0050] (4) Cultivation: The second air pipe 52 of the fermenter stops supplying air to the fermenter 2, and the first air pipe 51 of the fermenter starts supplying air to the fermenter 2, directly supplying air to the mixture of seed liquid and culture medium, which is conducive to the uniform dispersion of air in the mixture of seed liquid and culture medium.

[0051] The above technical solution optimizes the pipeline design of the microbial fermentation transfer device 100 by adding a second air pipe 42 for the seed tank and a second air pipe 52 for the fermentation tank, and connecting the first air pipe 41 for the seed tank and the first air pipe 51 for the fermentation tank to the main pipeline 3 respectively. During the transfer process in the seed tank 1, the first air pipe 41 for the seed tank supplies a small amount of air to the main pipeline 3, and the second air pipe 42 for the seed tank supplies air to the seed tank 1 to increase the tank pressure, driving the seed liquid into the main pipeline 3. Furthermore, since a small amount of air is continuously introduced into the main pipeline 3, oxygen deficiency in the seed liquid can be avoided in the main pipeline 3. During the transfer process in the fermentation tank 2, the second air pipe 52 for the fermentation tank supplies air to the fermentation tank 2 to regulate the tank pressure, which can avoid or reduce the foaming phenomenon of the culture medium caused by using the first air pipe 51 for the fermentation tank 2 to supply air.

[0052] Furthermore, when the seed solution is relatively viscous, the transfer speed via the main pipe 3 will be significantly reduced. In this case, before transfer, the air supply pipes of the fermenter's second air pipe 52 and the seed tank's second air pipe 42 are adjusted (for specific operation of adjusting the air pressure, refer to the air pressure adjustment operation in the above seed tank to fermenter transfer process) to ensure that the pressure in fermenter 2 is greater than that in seed tank 1, and the pressure difference between the two reaches a certain value. This allows the culture medium in fermenter 2 to be transported in reverse through the main pipe 3 to seed tank 1 for reverse transfer, diluting the seed solution before transfer. The seed solution and culture medium in seed tank 2 then enter fermenter 2. In other words, the microbial fermentation transfer device 100 of this scheme has more complete performance, wider application, and is also suitable for scenarios where the seed solution is relatively viscous.

[0053] It should be understood that, in order to ensure that the seed tank 1 and the fermentation tank 2 can be kept in a sterile state during use, the air input must be sterile air.

[0054] In one implementation, such as Figure 1 As shown, the microbial fermentation transfer device 100 also includes a steam input pipe 6, the outlet end of which is connected to the main pipeline 3. Specifically, when the seed tank 1 and the fermentation tank 2 need to be sterilized, steam is supplied to the main pipeline 3 through the steam input pipe 6, and then to the first air pipe 41 of the seed tank and the first air pipe 51 of the fermentation tank via the main pipeline 3. At the same time, the feed end 31 of the main pipeline 3 delivers steam to the bottom of the seed tank 1, and the discharge end 32 of the main pipeline 3 delivers steam to the bottom of the fermentation tank 2. The steam sterilizes the seed tank 1, the fermentation tank 2, the first air pipe 41 of the seed tank, the first air pipe 51 of the fermentation tank, and the main pipeline 3. The pipeline design of this scheme is simple, which can reduce the number of pipelines. The overall structure of the microbial fermentation transfer device 100 is simpler, reducing space occupation and cost.

[0055] Furthermore, the inlet end of the second air pipe 42 of the seed tank is connected to the first air pipe 41 of the seed tank. In this way, the first air pipe 41 and the second air pipe 42 of the seed tank share a section of pipeline, which helps to simplify the pipeline structure. Moreover, when steam sterilization is performed using the steam input pipe 6, steam can also enter the first air pipe 41 of the seed tank through the main pipe 3, and then enter the second air pipe 42 of the seed tank to sterilize the second air pipe 42 of the seed tank. There is no need to set up an additional steam connection branch pipe for the second air pipe 42 of the seed tank.

[0056] Furthermore, the first air pipe 41 of the seed tank is equipped with a first control valve 71 and a second control valve 72, and the second air pipe 42 of the seed tank is equipped with a third control valve 73. The first control valve 71 is located between the inlet end of the first air pipe 41 and the inlet end of the second air pipe 42, and the second control valve 72 is located between the inlet end of the second air pipe 42 and the outlet end of the first air pipe 41. Specifically, the first control valve 71 is used to open or close the first air pipe 41, the second control valve 72 is used to allow or prevent the first air pipe 41 from supplying air to the main pipe 3, and the third control valve 73 is used to open or close the second air pipe 42. It should be understood that when steam sterilization is performed using the steam input pipe 6, the first control valve 71 is closed to prevent steam from escaping from the inlet end of the first air pipe 41 (i.e., the port where air enters).

[0057] In one implementation, such as Figure 1 As shown, the inlet end of the second air pipe 52 of the fermenter is connected to the first air pipe 51 of the fermenter. In this way, the first air pipe 51 and the second air pipe 52 of the fermenter share a section of pipeline, which helps to simplify the pipeline structure. In addition, when steam sterilization is performed using the steam input pipe 6, the steam can also enter the first air pipe 51 of the fermenter through the main pipe 3, and then enter the second air pipe 52 of the fermenter to sterilize the second air pipe 52 of the fermenter. There is no need to set up an additional steam connection branch pipe for the second air pipe 52 of the fermenter.

[0058] Furthermore, the first air pipe 51 of the fermenter is equipped with a fourth control valve 74 and a fifth control valve 75, and the second air pipe 52 of the fermenter is equipped with a sixth control valve 76. The fourth control valve 74 is located between the inlet end of the first air pipe 51 and the inlet end of the second air pipe 52, and the fifth control valve 75 is located between the inlet end of the second air pipe 52 and the outlet end of the first air pipe 51. Specifically, the fourth control valve 74 is used to open or close the first air pipe 51, the fifth control valve 75 is used to allow or prevent the first air pipe 51 from supplying air to the main pipe 3, and the sixth control valve 76 is used to open or close the second air pipe 52. It should be understood that when steam sterilization is performed using the steam input pipe 6, the fourth control valve 74 is closed to prevent steam from escaping from the inlet end of the first air pipe 51 (i.e., the port where air enters).

[0059] Furthermore, the outlet end of the steam input pipe 6 is located between the outlet end of the first air pipe 41 of the seed tank and the outlet end of the first air pipe 51 of the fermenter, resulting in a more rational pipeline layout. After the steam in the steam input pipe 6 enters the main pipeline 3, it can be divided into two steam streams, one supplying the seed tank system and the other supplying the fermenter system. The main pipeline 3 is also equipped with a seventh control valve 77 and an eighth control valve 78. The seventh control valve 77 is located between the outlet end of the steam input pipe 6 and the outlet end of the first air pipe 41 of the seed tank, and the eighth control valve 78 is located between the outlet end of the steam input pipe 6 and the outlet end of the first air pipe 51 of the fermenter.

[0060] Furthermore, a ninth control valve 79 is provided on the steam input pipe 6 to control the opening or closing of the steam input pipe 6.

[0061] In one embodiment, the top of the seed tank 1 is connected to a first exhaust pipe 8, and a tenth control valve 7010 is provided on the first exhaust pipe 8; the top of the fermentation tank 2 is connected to a second exhaust pipe 9, and an eleventh control valve 7011 is provided on the second exhaust pipe 9. Specifically, when the seed tank 1 is sterilized or aerated, the tenth control valve 7010 is opened. During sterilization, the steam in the seed tank 1 is discharged through the first exhaust pipe 8. During aeration, excess air in the seed tank 1 is discharged through the first exhaust pipe 8 to maintain a constant tank pressure. When the seed tank 1 is used for seed transfer, the tenth control valve 7010 is closed to quickly increase the tank pressure of the seed tank 1. When fermenter 2 is sterilized or aerated, the eleventh control valve 7011 is opened. During sterilization, the steam in fermenter 2 is discharged through the second exhaust pipe 9. During aeration, the excess air in fermenter 2 is discharged through the second exhaust pipe 9 to maintain a constant tank pressure. When fermenter 2 is transferred, the eleventh control valve 7011 is opened. By adjusting the amount of air delivered by the second air pipe 52 of the fermenter and adjusting the aeration rate of the eleventh control valve 7011, the tank pressure of fermenter 2 is reduced to the required tank pressure value.

[0062] In one embodiment, the main pipeline 3 is provided with multiple discharge ends 32 (not shown in the figure), each discharge end 32 is matched with a fermentation tank 2, and each fermentation tank 2 is equipped with a first air pipe 51 and a second air pipe 52, which can provide seed transfer for multiple fermentation tanks 2.

[0063] In one embodiment, a first exhaust valve 7021 is provided upstream of the first control valve 71, a second exhaust valve 7022 is provided upstream of the third control valve 73, a third exhaust valve 7023 is provided upstream of the seventh control valve 77, a fourth exhaust valve 7024 is provided upstream of the eighth control valve 78, a fifth exhaust valve 7025 is provided upstream of the sixth control valve 76, and a sixth exhaust valve 7026 is provided upstream of the fourth control valve 74. The first exhaust valve 7021, second exhaust valve 7022, third exhaust valve 7023, fourth exhaust valve 7024, fifth exhaust valve 7025, and sixth exhaust valve 7026 are all used for steam end drainage to solve the problem of sterilization dead zones. It should be understood that "upstream" in this document refers to the position closer to the steam inlet pipe 6 in the direction of steam flow.

[0064] In one specific embodiment, the working steps of the seed tank 1 include:

[0065] (1) Sterilize seed tank 1; close the first control valve 71, and keep the second control valve 72, the third control valve 73, the seventh control valve 77, the ninth control valve 79, and the tenth control valve 7010 in the open state. Steam enters the main pipe 3 through the steam input pipe 6. One steam enters the bottom of seed tank 1 through the main pipe 3 to sterilize the culture medium, and then is discharged from the first exhaust pipe 8. Another steam enters the first air pipe 41 and the second air pipe 42 of the seed tank to sterilize the first air pipe 41 and the second air pipe 42 of the seed tank.

[0066] (2) Ventilation: After sterilization, close the seventh control valve 77 and the third control valve 73, open the first control valve 71, and keep the second control valve 72 open. Air enters the bottom of the seed tank 1 through the first air pipe 41 of the seed tank and is finally discharged from the first exhaust pipe 8.

[0067] (3) Inoculation and cultivation: The seed tank 1 is inoculated with microbial strains using the differential pressure method or the fire ring protection method. The required amount of air is continuously supplied to the seed tank 1 through the first air pipe 41 of the seed tank. The microbial strains grow and mature into a certain number of mycelia and bacterial counts to obtain seed liquid.

[0068] (4) Transplanting: Reduce the air flow of the second control valve 72. A small amount of air enters the main pipe 3 from the first air pipe 41 of the seed tank. Open the third control valve 73. Air enters the upper part of the seed tank 1 from the second air pipe 42 of the seed tank to force the seeds in the seed tank 1 into the main pipe 3.

[0069] The working steps of fermenter 2 include:

[0070] (1) Sterilization of fermenter 2: Close the fourth control valve 74, and keep the fifth control valve 75, the sixth control valve 76, the eighth control valve 78, the ninth control valve 79, and the eleventh control valve 7011 in the open state. Steam enters the main pipe 3 through the steam input pipe 6. One path of steam enters the bottom of fermenter 2 through the main pipe 3 to sterilize the culture medium, and then is discharged from the second exhaust pipe 9. Another path of steam enters the first air pipe 51 and the second air pipe 52 of fermenter to sterilize the first air pipe 51 and the second air pipe 52 of fermenter.

[0071] (2) Ventilation: After sterilization, close the eighth control valve 78 and the sixth control valve 76, open the fourth control valve 74, and keep the fifth control valve 75 open. Air enters the bottom of the fermenter 2 through the first air pipe 51 of the fermenter and is finally discharged from the second exhaust pipe 9.

[0072] (3) Transplanting: Close the fifth control valve 75, open the sixth control valve 76, adjust the exhaust volume of the eleventh control valve 7011, and the seed liquid enters the bottom of the fermentation tank 2 from the main pipe 3. Transplanting is completed. Close the eighth control valve 78.

[0073] (4) Cultivation: Close the sixth control valve 76, open the fifth control valve 75, and the first air pipe 51 of the fermenter delivers air to the bottom of the fermenter 2. Adjust the eleventh control valve 7011 to ensure constant tank pressure.

[0074] It should be understood that the above embodiments are exemplary and are not intended to encompass all possible implementations included in the claims. Various modifications and changes can be made to the above embodiments without departing from the scope of this disclosure. Similarly, the various technical features of the above embodiments can be arbitrarily combined to form other embodiments of this utility model that may not be explicitly described. Therefore, the above embodiments only illustrate several implementations of this utility model and do not limit the scope of protection of this utility model patent.

Claims

1. A microbial fermentation transfer device, characterized in that, The microbial fermentation transfer device (100) includes: Seed container (1), which is used to cultivate microbial strains; Fermentation tank (2), which is used to ferment seed liquid; The main pipe (3) has its feed end (31) inserted from the top of the seed tank (1) and extending into the bottom of the seed tank (1), and its discharge end (32) inserted from the top of the fermentation tank (2) and extending into the bottom of the fermentation tank (2). The first air pipe (41) of the seed tank is connected to the main pipe (3) at its outlet end; The second air tube (42) of the seed tank has its outlet end inserted from the upper part of the seed tank (1) and located in the upper part of the seed tank (1); The first air pipe (51) of the fermenter is connected at its outlet to the main pipe (3); The second air pipe (52) of the fermenter has its outlet end inserted from the upper part of the fermenter (2) and located in the upper part of the fermenter (2); Before transplanting, the first air pipe (41) of the seed tank supplies air to the seed tank (1), and the first air pipe (51) of the fermentation tank supplies air to the fermentation tank (2); During the transplanting process, the second air pipe (42) of the seed tank supplies air to the seed tank (1), the second air pipe (52) of the fermentation tank supplies air to the fermentation tank (2), and the seed liquid in the seed tank (1) is transported to the fermentation tank (2) through the main pipe (3).

2. The microbial fermentation transfer device according to claim 1, characterized in that, The microbial fermentation transfer device (100) also includes a steam input pipe (6), the outlet of which is connected to the main pipe (3).

3. The microbial fermentation transfer device according to claim 2, characterized in that, The inlet end of the second air pipe (42) of the seed tank is connected to the first air pipe (41) of the seed tank.

4. The microbial fermentation transfer device according to claim 3, characterized in that, The first air pipe (41) of the seed tank is provided with a first control valve (71) and a second control valve (72), and the second air pipe (42) of the seed tank is provided with a third control valve (73); the first control valve (71) is located between the inlet end of the first air pipe (41) of the seed tank and the inlet end of the second air pipe (42) of the seed tank, and the second control valve (72) is located between the inlet end of the second air pipe (42) of the seed tank and the outlet end of the first air pipe (41) of the seed tank.

5. The microbial fermentation transfer device according to claim 3, characterized in that, The inlet end of the second air pipe (52) of the fermenter is connected to the first air pipe (51) of the fermenter.

6. The microbial fermentation transfer device according to claim 5, characterized in that, The first air pipe (51) of the fermenter is provided with a fourth control valve (74) and a fifth control valve (75), and the second air pipe (52) of the fermenter is provided with a sixth control valve (76); the fourth control valve (74) is located between the inlet end of the first air pipe (51) of the fermenter and the inlet end of the second air pipe (52) of the fermenter, and the fifth control valve (75) is located between the inlet end of the second air pipe (52) of the fermenter and the outlet end of the first air pipe (51) of the fermenter.

7. The microbial fermentation transfer device according to claim 5, characterized in that, The outlet end of the steam input pipe (6) is located between the outlet end of the first air pipe (41) of the seed tank and the outlet end of the first air pipe (51) of the fermentation tank. The main pipe (3) is also equipped with a seventh control valve (77) and an eighth control valve (78). The seventh control valve (77) is located between the outlet end of the steam input pipe (6) and the outlet end of the first air pipe (41) of the seed tank. The eighth control valve (78) is located between the outlet end of the steam input pipe (6) and the outlet end of the first air pipe (51) of the fermenter.

8. The microbial fermentation transfer device according to claim 7, characterized in that, The steam input pipe (6) is equipped with a ninth control valve (79).

9. The microbial fermentation transfer apparatus according to any one of claims 1-8, characterized in that, The top of the seed tank (1) is connected to a first exhaust pipe (8), and the first exhaust pipe (8) is equipped with a tenth control valve (7010); the top of the fermentation tank (2) is connected to a second exhaust pipe (9), and the second exhaust pipe (9) is equipped with an eleventh control valve (7011).

10. The microbial fermentation transfer apparatus according to any one of claims 1-8, characterized in that, The main pipeline (3) is provided with multiple discharge ends (32), each discharge end (32) is matched with a fermentation tank (2), and each fermentation tank (2) is equipped with a first air pipe (51) and a second air pipe (52).

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