Incubator for cell culture

Abstract

The utility model relates to an incubator for cell culture, which comprises an incubator body with an opening at the top and an incubator cover detachably arranged at the top of the incubator body and used for closing the opening, rotating holes are arranged on the inner walls of the front side and the rear side in the incubator body, and rotating bolts are rotatably connected in the two rotating holes. The opposite ends of the two rotating bolts are connected with the same movable plate, a double-shaft motor is fixedly arranged on the bottom wall in the incubator, cams are installed on two output shafts of the double-shaft motor, protruding heads of the two cams are oppositely arranged, and the outer side walls of the two cams make contact with the bottom sides of the left end and the right end of the movable plate. A left clamping plate and a right clamping plate which are used for fixing a culture dish are arranged at the top of the movable plate, a double-shaft motor is controlled to work, two output shafts of the double-shaft motor respectively drive two cams to rotate, raised heads of the two cams are oppositely arranged, so that the raised heads are respectively in contact with the movable plate, and the left side and the right side of the movable plate continuously swing up and down; the culture solution in the culture dish flows and is distributed at the bottom of the culture dish.

Description

TECHNICAL FIELD

[0001] The utility model relates to stem cell culture technical field, concretely relates to a culture box for cell culture. BACKGROUND

[0002] Stem cells refer to a kind of cells with self-renewal and differentiation capacity, they can differentiate into different types of cells, and can constantly self-replicate to maintain its quantity, stem cells have multi-directional differentiation potential, that is, can differentiate into multiple different types of cells, such as muscle cells, nerve cells, heart cells etc., and can also produce the same type of cells, called unidirectional differentiation potential, due to its strong differentiation potential and self-renewal capacity, therefore, it has important role in medical research and treatment, stem cell culture is the process of placing stem cells into culture dish containing appropriate nutrient components and environment to promote its growth and self-renewal.

[0003] The existing culture dish is usually placed in the incubator for culture, when culturing, usually need to add drop culture solution into the culture dish, but due to the drop amount of culture solution needs to be considered according to the inside situation of culture dish when adding culture solution, if the drop amount is less, the inside culture dish often cannot evenly distribute culture solution, need manual to adjust the angle of culture dish to make the culture solution in culture dish evenly distributed in the bottom of culture dish, it is more troublesome. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a culture box for cell culture, solve the problem that the culture device in prior art needs manual to adjust the angle of culture dish to make the culture solution in culture dish evenly distributed in the bottom of culture dish.

[0005] To solve the above technical problem, the utility model adopts the following technical scheme:

[0006] A culture box for cell culture, including the culture box with opening in top and the box cover that can be detachably arranged on the top of culture box and used for closing the opening, the rotating hole is arranged on the inner wall of culture box at front and back sides, the rotating bolt is rotatably connected in the two rotating holes, the same movable plate is connected to the opposite end of the two rotating bolts, the double-shaft motor is fixedly arranged on the bottom wall in culture box, the two output shafts of double-shaft motor are respectively arranged towards the left and right side walls of culture box, and the cam is installed on the two output shafts, the convex heads of the two cams are oppositely arranged, and the outer side walls of the two cams are in contact with the bottom side of the left and right ends of movable plate.

[0007] Further technical solutions are that the top of the movable plate is provided with a transmission groove with two ends respectively facing the left and right side walls of the incubator, a sliding rod is fixedly arranged in the transmission groove, a left supporting spring and a right supporting spring are respectively sleeved on the outer sides of the two ends of the sliding rod, one end of the left supporting spring is fixedly connected with the left groove wall of the transmission groove, and the other end is connected with a left sliding block sleeved on the sliding rod; one end of the right supporting spring is fixedly connected with the right groove wall of the transmission groove, and the other end is connected with a right sliding block sleeved on the sliding rod; and the top ends of the left and right sliding blocks pass through the transmission groove and are fixedly connected with the bottom ends of the left and right clamping plates respectively.

[0008] Still further technical solutions are that the left and right clamping plates are respectively provided with a silica gel pad on the side facing each other.

[0009] Still further technical solutions are that the box opening of the incubator is provided with a sealing strip, the sealing strip is arranged around the inner side wall of the incubator, the top of the side wall of the incubator is provided with a connecting hole, the bottom side of the box cover is provided with a plug matched with the connecting hole, and the opposite ends of the box cover are provided with handles.

[0010] Still further technical solutions are that the inner side wall of the incubator is provided with an ultraviolet lamp.

[0011] Compared with the prior art, the incubator has the advantages that:

[0012] By controlling the operation of the double-shaft motor, the two output shafts of the double-shaft motor drive the two cams to rotate, the protrusions of the two cams are oppositely arranged to be in contact with the movable plate, the movable plate is driven to reciprocatingly rotate around the rotating pin under the cooperation of the rotating pin and the rotating hole, the left and right sides of the movable plate are continuously up and down swung, the culture dishes on the movable plate are shaken, the culture solution in the culture dishes is distributed on the bottom of the culture dishes, and the culture solution in the culture dishes is prevented from being precipitated due to long-time static culture, so that the normal culture of tumor cells is facilitated. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 It is a structure diagram of the incubator for cell culture.

[0014] Figure 2 It is an exploded view of the incubator.

[0015] Legend: 1-incubator, 2-box cover, 3-rotating hole, 4-rotating pin, 5-movable plate, 6-double-shaft motor, 7-cam, 8-output shaft, 9-left clamping plate, 10-right clamping plate, 11-transmission groove, 12-sliding rod, 13-left supporting spring, 14-right supporting spring, 15-left sliding block, 16-right sliding block, 17-silica gel pad, 18-sealing strip, 19-connecting hole, 20-plug, 21-handle, 22-ultraviolet lamp, 23-mounting hole, 24-transparent glass. Detailed Implementation

[0016] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.

[0017] Example 1

[0018] See Figure 1 , Figure 2 The present invention discloses a cell culture chamber, comprising a culture chamber 1 with an opening at the top and a detachable cover 2 on the top of the culture chamber 1 for closing the opening. Rotating holes 3 are provided on the inner walls of the front and rear sides of the culture chamber 1. Rotating bolts 4 are rotatably connected to each of the two rotating holes 3. The two rotating bolts 4 are connected to the same movable plate 5 at opposite ends. A dual-axis motor 6 is fixedly mounted on the bottom wall of the culture chamber 1. The two output shafts 8 of the dual-axis motor 6 are respectively positioned facing the left and right side walls of the culture chamber 1, and cams 7 are mounted on each of the two output shafts 8. The protrusions of the two cams 7 are arranged oppositely, and the outer walls of the two cams 7 are in contact with the bottom sides of the left and right ends of the movable plate 5. A left clamp 9 and a right clamp 10 for fixing culture dishes are provided on the top of the movable plate 5.

[0019] The use of a control box to control the rotation of the dual-axis motor 6 is existing technology and will not be described in detail here. It should be noted that when the dual-axis motor 6 drives the convex heads of the two cams 7 to be in a horizontal orientation, the bottom side of the movable plate 5 contacts the side wall of the two cams 7 respectively. At this time, the top surface of the movable plate 5 is horizontal. The structure in which the rotating hole 3 and the rotating bolt 4 are connected by rotating bearings is common knowledge to those skilled in the art, and those skilled in the art can directly obtain the corresponding installation relationship and structure based on common knowledge.

[0020] In use, the culture dish is first fixed to the movable plate 5 using the left clamp 9 and the right clamp 10. The lid 2 is then closed to prevent external bacteria from entering the incubator 1. The dual-axis motor 6 is then activated, and its two output shafts 8 drive two cams 7 to rotate. The convex heads of the two cams 7 are arranged in opposite directions so that they contact the movable plate 5. The movable plate 5 rotates back and forth around the rotating bolt 4 with the rotating hole 3 as the axis of rotation. This causes the movable plate 5 to swing up and down continuously on both sides, which in turn causes the culture dish on the movable plate 5 to shake. This allows the culture medium in the culture dish to flow and distribute at the bottom of the culture dish, while preventing sedimentation of the culture medium due to prolonged stillness, thus facilitating the normal culture of tumor cells.

[0021] Example 2

[0022] On the basis of the foregoing embodiments, referring to Figure 1 、 Figure 2 As shown in the figure, the top of the movable plate 5 is provided with a transmission groove 11 with two ends respectively facing the left and right side walls of the incubator 1, and a sliding rod 12 is fixedly arranged in the transmission groove 11. A left supporting spring 13 and a right supporting spring 14 are respectively sleeved on the outer sides of the two ends of the sliding rod 12. One end of the left supporting spring 13 is fixedly connected with the left groove wall of the transmission groove 11, and the other end is connected with a left sliding block 15 which is sleeved on the sliding rod 12. One end of the right supporting spring 14 is fixedly connected with the right groove wall of the transmission groove 11, and the other end is connected with a right sliding block 16 which is sleeved on the sliding rod 12. The top ends of the left and right sliding blocks 15 and 16 respectively pass through the transmission groove 11 and are fixedly connected with the bottom ends of the left and right clamping plates 9 and 10.

[0023] When the culture dish is aligned between the left and right clamping plates 9 and 10 and is pressed down, the culture dish is pressed to make the left and right clamping plates 9 and 10 move away from each other, which drives the left and right sliding blocks 15 and 16 to slide along the sliding groove and move away from each other, respectively pressing the left and right supporting springs 13 and 14, until the bottom side of the culture dish is in contact with the top side of the movable plate 5. At this time, the left and right supporting springs 13 and 14 are elastically pressed against the left and right sliding blocks 15 and 16, which in turn drives the left and right clamping plates 9 and 10 to clamp and limit the culture dish, thereby fixing the culture dish. By arranging the sliding rod 12, the left and right sliding blocks 15 and 16, the left and right supporting springs 13 and 14 can be limited to avoid sliding out of the transmission groove 11.

[0024] As a preferred embodiment, referring to Figure 1 、 Figure 2 As shown in the figure, the left and right clamping plates 9 and 10 are respectively provided with a silica gel pad 17 on the side facing each other. By arranging the silica gel pad 17, the silica gel pad 17 can avoid damaging the culture dish when clamping and fixing the culture dish, and at the same time, the silica gel pad 17 can increase the friction between the culture dish, thereby better fixing the culture dish.

[0025] As a preferred embodiment, referring to Figure 1 、 Figure 2As shown, the box mouth of the incubator 1 is provided with a sealing strip 18, which is arranged around the inner side wall of the incubator 1. It should be noted that the top of the sealing strip 18 is higher than the top of the incubator 1. The top of the sealing strip 18 is provided with a connecting hole 19, and the bottom side of the box cover 2 is provided with a plug 20 matched with the connecting hole 19. The box cover 2 is provided with a handle 21 at each end. When the box cover 2 is installed on the top of the incubator 1, the plug 20 is inserted into the connecting hole 19, and the top of the sealing strip 18 is tightly attached to the bottom side of the box cover 2. When installing the box cover 2, the plug 20 is aligned with the corresponding connecting hole 19, and the box cover 2 is installed downward, so that the plug 20 is inserted into the connecting hole 19. At this time, the top of the sealing strip 18 is tightly attached to the bottom side wall of the box cover 2, which can prevent bacteria in the outside environment from entering the incubator 1 through the gap between the box cover 2 and the incubator 1, thereby affecting the culture of stem cells. By arranging the plug 20 and the connecting hole 19, the box cover 2 can be stably installed on the bottom of the incubator 1, and the box cover 2 can be easily disassembled. When the box cover 2 needs to be disassembled, the handle 21 on the box cover 2 is pulled upward, so that the plug 20 slides out of the connecting hole 19, and the box cover 2 can be disassembled.

[0026] As a preferred embodiment, refer to Figure 1 、 Figure 2 As shown, the inner side wall of the incubator 1 is provided with an ultraviolet lamp 22. Before using the device, the ultraviolet lamp 22 can be turned on to sterilize and disinfect the inside of the incubator 1, so that the inside of the incubator 1 is in a sterile state, thereby ensuring that the subsequent cell culture will not be affected. It should be noted that the ultraviolet lamp 22 can be controlled by the control box, which is known in the art and will not be described here.

[0027] As a preferred embodiment, refer to Figure 1 As shown, the top of the box cover 2 is provided with a mounting hole 23 penetrating through the upper and lower sides thereof. The mounting hole 23 is provided with a transparent glass 24. The transparent glass 24 can be used to observe the inside of the incubator 1.

[0028] Although the present application has been described with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the principles and spirit of the disclosure. More particularly, various modifications and improvements can be made to the components of the subject combination layout and / or the layout itself within the teachings of the present disclosure, drawings, and claims. In addition to modifications and improvements to the components and / or layout, other uses will also be apparent to those skilled in the art.

Claims

1. A cell culture chamber, comprising a chamber (1) with an opening at the top and a detachable cover (2) disposed at the top of the chamber (1) for closing the opening, characterized in that: Rotating holes (3) are provided on the inner walls of the front and rear sides of the incubator (1). Rotating bolts (4) are rotatably connected in both rotating holes (3). The two rotating bolts (4) are connected to the same movable plate (5) at one end facing each other. A dual-axis motor (6) is fixedly installed on the bottom wall of the incubator (1). The two output shafts (8) of the dual-axis motor (6) are respectively set towards the left and right side walls of the incubator (1), and cams (7) are installed on both output shafts (8). The protrusions of the two cams (7) are set opposite to each other, and the outer side walls of the two cams (7) are in contact with the bottom sides of the left and right ends of the movable plate (5). The top of the movable plate (5) is provided with a left clamp (9) and a right clamp (10) for fixing the culture dish.

2. The cell culture incubator according to claim 1, characterized in that: The top of the movable plate (5) is provided with a transmission groove (11) with both ends facing the left and right side walls of the incubator (1). A slide rod (12) is fixedly provided in the transmission groove (11). A left support spring (13) and a right support spring (14) are respectively sleeved on the outer sides of both ends of the slide rod (12). One end of the left support spring (13) is fixedly connected to the left groove wall of the transmission groove (11), and the other end is connected to a left slider (15) that is slidably sleeved on the slide rod (12). One end of the right support spring (14) is fixedly connected to the right groove wall of the transmission groove (11), and the other end is connected to a right slider (16) that is slidably sleeved on the slide rod (12). The top ends of the left slider (15) and the right slider (16) protrude from the transmission groove (11) and are fixedly connected to the bottom ends of the left clamping plate (9) and the right clamping plate (10) respectively.

3. The cell culture incubator according to claim 1, characterized in that: The left clamp (9) and the right clamp (10) are each provided with a silicone pad (17) on the opposite side.

4. The cell culture incubator according to claim 1, characterized in that: The incubator (1) has a sealing strip (18) at the opening. The sealing strip (18) surrounds the inner side wall of the incubator (1). The sealing strip (18) has a connection hole (19) at the top of the side wall of the incubator (1). The bottom side of the cover (2) has a plug (20) that matches the connection hole (19). The cover (2) has handles (21) at both ends.

5. The cell culture incubator according to claim 1, characterized in that: An ultraviolet lamp (22) is installed on the inner wall of the incubator (1).

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