Liquid nitrogen box suitable for embryo freezing

Abstract

The liquid nitrogen box comprises a liquid nitrogen box body, the liquid nitrogen box body comprises an inner freezing heat preservation cavity and an outer protection shell, a first hole frame used for containing a freezing pipe is fixedly arranged at the bottom of the inner side of the inner freezing heat preservation cavity, and a second supporting assembly used for supporting the upper portion of the freezing pipe is arranged on the upper portion of the first hole frame. The second supporting assembly is connected with an adjusting assembly on the side wall of the inner freezing heat preservation cavity so as to adjust the vertical height of the second supporting assembly relative to the first hole frame, and a double-layer heat preservation cover is arranged in the liquid nitrogen box body and comprises an outer cover arranged on the upper portion of the liquid nitrogen box body and an inner heat preservation cover in threaded connection with the inner freezing heat preservation cavity. According to the utility model, the second supporting assembly with adjustable height is arranged, so that the liquid nitrogen box can accommodate embryo freezing tubes with different lengths, and a sample is ensured to be completely immersed in a low-temperature environment; meanwhile, a double-layer heat preservation cover and a composite heat insulation structure are adopted, heat exchange is effectively restrained, the heat preservation performance is improved, and liquid nitrogen consumption and sample storage risks are reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to embryo freezing technical field, concretely relates to a liquid nitrogen box suitable for embryo freezing. BACKGROUND

[0002] In the field of assisted reproduction, biological sample library and life science research, low temperature cryopreservation is the key technology for long-term storage of precious biological samples such as embryos and stem cells, and liquid nitrogen provides a stable ultralow temperature environment, and the liquid nitrogen box for storing samples is the core container.

[0003] At present, the conventional liquid nitrogen box is usually standardized, the height of the internal storage cavity is usually designed for the general 2ml standard freezing tube, however, with the development of technology, specific application scenarios (such as some in vivo embryo transfer technology) need to use longer special embryo freezing tubes, and the length often exceeds the effective height of the internal cavity of the conventional liquid nitrogen box, and the long tube is placed in the standard liquid nitrogen box, which causes the cap to be partially exposed to the liquid surface or unable to be placed vertically, not only can not ensure that the sample is completely immersed in the low-temperature gas phase or liquid phase, but also affects the freezing uniformity and storage safety, and there is a risk of tube body tilting, collision and even dumping, which is easy to cause irreversible sample damage. SUMMARY

[0004] In order to solve the above problems, the utility model provides a liquid nitrogen box suitable for embryo freezing.

[0005] The utility model is realized through the following technical schemes:

[0006] A liquid nitrogen box suitable for embryo freezing, comprising a liquid nitrogen box body, the liquid nitrogen box body comprises an inner freezing and heat preservation cavity and an outer protective shell, a first hole frame for placing a freezing tube is fixedly arranged on the inner side bottom of the inner freezing and heat preservation cavity, a second support assembly for supporting the upper part of the freezing tube is arranged on the upper part of the first hole frame, the second support assembly is connected with an adjusting assembly on the side wall of the inner freezing and heat preservation cavity, so as to adjust the vertical height thereof relative to the first hole frame, a double-layer heat preservation cover is arranged in the liquid nitrogen box body, the double-layer heat preservation cover comprises an outer cover arranged on the upper part of the liquid nitrogen box body and an inner heat preservation cover threadedly connected with the inner freezing and heat preservation cavity.

[0007] Further, the second support assembly comprises a second hole frame above the first hole frame, the left and right sides of the second hole frame are respectively fixedly provided with a connecting box, a square limiting rod is slidably connected in the connecting box, the two sides of the square limiting rod are respectively connected with a sliding block, a first sliding groove for the movement of the sliding block is formed in the connecting box, a pushing rod is fixedly connected to the upper surface of the square limiting rod, a second sliding groove for the movement of the pushing rod is formed in the upper part of the connecting box, and a spring is fixedly connected between the square limiting rod and the connecting box.

[0008] Further, the adjusting assembly comprises a slide fixed to the inner side walls of the inner freezing and heat preservation cavity, a connecting rod is slidably connected in the slide, a plurality of limiting holes matched with the square limiting rods are formed in the connecting rod, and a hanging ring facilitating lifting is arranged on the upper portion of the connecting rod.

[0009] Further, a plurality of first positioning holes are formed in the first hole frame, a plurality of second positioning holes are formed in the second hole frame, and the first positioning holes and the second positioning holes are coaxially arranged in the vertical direction and correspond to each other to jointly accommodate and fix the upper and lower ends of the same frozen tube.

[0010] Further, the inner freezing and heat preservation cavity is made of stainless steel, and a plurality of layers of heat insulation material layers are arranged in the interlayer between the inner freezing and heat preservation cavity and the outer protective shell.

[0011] Further, the inner heat preservation cover and the outer cover are respectively provided with handles facilitating opening.

[0012] Further, a handle is hingedly connected to the outer side of the outer protective shell.

[0013] Compared with the prior art, the beneficial effects of the utility model are that: the second supporting assembly with adjustable vertical height is arranged, so that the liquid nitrogen box can stably accommodate special embryo freezing tubes with different lengths, and it is ensured that the sample is completely immersed in the low-temperature environment; at the same time, the double-layer heat preservation cover and the composite heat insulation structure are adopted, heat exchange is effectively inhibited, the heat preservation performance and the temperature stability are significantly improved, and the liquid nitrogen consumption and the sample storage risk are reduced. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 is a structural schematic diagram of the utility model;

[0015] Figure 2 is a partial sectional view schematic diagram of Figure 1 ;

[0016] Figure 3 is a partial enlarged schematic diagram of Figure 2 ;

[0017] Figure 4 is a front view schematic diagram of Figure 2 ;

[0018] In the drawing: the liquid nitrogen box body 1, the inner freezing and heat preservation cavity 2, the outer protective shell 3, the first hole frame 4, the first positioning hole 41, the second supporting assembly 5, the outer cover 6, the inner heat preservation cover 7, the second hole frame 8, the second positioning hole 81, the connecting box 9, the square limiting rod 10, the sliding block 11, the first sliding groove 12, the push rod 13, the second sliding groove 14, the spring 15, the slide 16, the connecting rod 17, the limiting hole 18, the hanging ring 19, the plurality of layers of heat insulation material layers 20, the handle 21, and the handle 22. DETAILED DESCRIPTION

[0019] The utility model will be described in further detail below in combination with the drawings and specific embodiments:

[0020] As Figure 1 , 2 , 3, 4 shows, a kind of liquid nitrogen box suitable for embryo freezing, including liquid nitrogen box body 1, liquid nitrogen box body 1 includes inner freezing heat preservation cavity 2 with outer protective shell 3, first hole frame 4 of placing frozen tube is fixedly arranged on inner freezing heat preservation cavity 2 inside bottom, second support assembly 5 for supporting the upper portion of frozen tube is arranged on the upper portion of first hole frame 4, second support assembly 5 is connected with the adjusting assembly on the side wall of inner freezing heat preservation cavity 2, to adjust its vertical height relative to first hole frame 4, double-layer heat preservation cover is provided in the inside of liquid nitrogen box body 1, double-layer heat preservation cover includes outer cover 6 arranged in the upper portion of liquid nitrogen box body 1 and inner heat preservation cover 7 with inner freezing heat preservation cavity 2 screw connection.

[0021] As Figure 2 , 3 It is shown that second support assembly 5 includes second hole frame 8 in the first hole frame 4 directly above, second hole frame 8 left and right sides are fixedly connected with connecting box 9, corresponding connecting box 9 is slidably connected with square limiting rod 10, slide block 11 is connected with the two sides of square limiting rod 10 respectively, first sliding slot 12 for the movement of slide block 11 is opened in the inside of connecting box 9, the upper surface of square limiting rod 10 is fixedly connected with the lever 13, the second sliding slot 14 for the movement of lever 13 is correspondingly opened in the upper portion of connecting box 9, spring 15 is fixedly connected between square limiting rod 10 and connecting box 9.

[0022] As Figure 2 , 3 It is shown that adjusting assembly includes slide 16 fixed on the side wall of inner freezing heat preservation cavity 2 both sides, corresponding slide 16 is slidably connected with connecting rod 17, a plurality of limiting holes 18 matched with square limiting rod 10 are opened in the upper portion of connecting rod 17, hanging ring 19 for facilitating lifting is arranged on the upper portion of connecting rod 17.

[0023] As Figure 2 , 3 , 4 shows, a plurality of first positioning holes 41 are opened in first hole frame 4, a plurality of second positioning holes 81 are opened in second hole frame 8, first positioning hole 41 and second positioning hole 81 are coaxially arranged in vertical direction to accommodate and fix the upper and lower ends of the same frozen tube.

[0024] As Figure 2 , 4 It is shown that inner freezing heat preservation cavity 2 is made of stainless steel material, and a plurality of layers of heat insulation material layers 20 are arranged in the interlayer between inner freezing heat preservation cavity 2 and outer protective shell 3.

[0025] As Figure 1 , 2As shown in Figure 4, the inner insulation cover 7 and the outer cover 6 are respectively provided with handles 21 for easy opening.

[0026] like Figure 1 , 2 As shown in Figures 4 and 5, a handle 22 is hinged to the outer side of the outer protective shell 3.

[0027] The implementation principle of a liquid nitrogen box suitable for embryo freezing in this application embodiment is as follows:

[0028] In use, first lift the handle 22 to raise the liquid nitrogen box body 1 to the working position. Then, open the handle 21 on the outer cover 6. Turn the handle 21 on the inner insulation cover 7 to open the inner freezing insulation cavity 2. Next, use the external hook to hang on the hanging ring 19 on the connecting rod 17 to raise the connecting rod 17 to the outside of the inner freezing insulation cavity 2. Then, move the lever 13 on the connecting boxes 9 on both sides to pull the square limiting rod 10 out of the limiting hole 18. After the square limiting rod 10 is pulled out of the limiting hole 18, the spring 15 connecting the square limiting rod 10 and the connecting box 9 will be compressed, thus generating a rebound force. Then, adjust the distance between the second hole bracket 8 and the first hole bracket 4 according to the length of the freezing tube used on site. After adjusting, release the lever 13. The spring force generated by the spring 15 will push the square limiting rod 10 into the corresponding limiting hole 18. Then, vertically insert the lower end of the embryo cryopreservation tube into the second positioning hole 81 on the second bracket 8, which is coaxial with it. At this time, the cap of the embryo cryopreservation tube will be locked by the second positioning hole 81 and will be in a vertically downward state. Then, place the second bracket 8 at a fixed distance into the inner cryopreservation chamber 2 along the slide 16. At this time, the lower part of the embryo cryopreservation tube will enter the first positioning hole 41 corresponding to the first bracket 4, so that the cryopreservation tube is precisely and vertically fixed between the two, ensuring that its entire length can be stably kept in the low temperature protection area.

[0029] After completing the sample storage and retrieval operation, first tighten the inner insulation cover 7 to seal the opening of the inner freezing insulation cavity 2, and then close the outer cover 6. This double sealing structure can effectively block the intrusion of external heat and minimize the evaporation of liquid nitrogen. At the same time, the synergistic effect of the multi-layer heat insulation material layer 20 between the inner freezing insulation cavity 2 and the outer protective shell 3 provides long-term and stable heat insulation performance for the entire storage environment.

[0030] When the liquid nitrogen container needs to be moved, it can be safely and conveniently transported by unfolding and holding the handle 22 that is hinged to the outside of the outer protective shell 3. The entire process solves the problems of adaptability and temperature stability of long embryo cryopreservation tube storage through the highly adjustable collaborative support structure and composite insulation design.

[0031] The basic principle, main features and advantages of the present application are shown and described above. Those skilled in the art should understand that the present application is not limited by the above examples, and the above examples and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application. The scope of protection of the present application is defined by the appended claims and their equivalents.

Claims

1. A liquid nitrogen box suitable for embryo freezing, comprising a liquid nitrogen box body (1), characterized in that: The liquid nitrogen box body (1) comprises an inner freezing insulation cavity (2) and an outer protective shell (3), a first hole frame (4) for placing a freezing tube is fixedly arranged on the inner side bottom of the inner freezing insulation cavity (2), a second supporting assembly (5) for supporting the upper part of the freezing tube is arranged on the upper part of the first hole frame (4), the second supporting assembly (5) is connected with an adjusting assembly on the side wall of the inner freezing insulation cavity (2) to adjust the vertical height thereof relative to the first hole frame (4), a double-layer insulation cover is arranged in the liquid nitrogen box body (1), the double-layer insulation cover comprises an outer cover (6) arranged on the upper part of the liquid nitrogen box body (1) and an inner insulation cover (7) threadedly connected with the inner freezing insulation cavity (2), the second supporting assembly (5) comprises a second hole frame (8) located directly above the first hole frame (4), connecting boxes (9) are fixedly arranged on the left side and the right side of the second hole frame (8) respectively, square limiting rods (10) are slidably connected in the connecting boxes (9) correspondingly, the square limiting rods (10) are connected with sliding blocks (11) respectively on the two sides thereof, first sliding grooves (12) for the movement of the sliding blocks (11) are formed in the connecting boxes (9), a pulling rod (13) is fixedly connected to the upper surface of the square limiting rod (10), second sliding grooves (14) for the movement of the pulling rod (13) are formed in the upper part of the connecting boxes (9), springs (15) are fixedly connected between the square limiting rods (10) and the connecting boxes (9), and the adjusting assembly comprises sliding rails (16) fixedly arranged on the side walls of the inner freezing insulation cavity (2) on the two sides, connecting rods (17) are slidably connected in the sliding rails (16) correspondingly, a plurality of limiting holes (18) matched with the square limiting rods (10) are formed in the connecting rods (17), and hanging rings (19) facilitating lifting are arranged on the upper part of the connecting rods (17).

2. A liquid nitrogen box suitable for embryo freezing according to claim 1, characterized in that: A plurality of first positioning holes (41) are formed in the first hole frame (4), a plurality of second positioning holes (81) are formed in the second hole frame (8), and the first positioning holes (41) and the second positioning holes (81) are coaxially and correspondingly arranged in the vertical direction to jointly accommodate and fix the upper and lower ends of the same freezing tube.

3. The liquid nitrogen box suitable for embryo freezing according to claim 1, characterized in that: The inner freezing insulation cavity (2) is made of stainless steel, and a plurality of layers of heat insulation material layers (20) are arranged in the interlayer between the inner freezing insulation cavity (2) and the outer protective shell (3).

4. The liquid nitrogen box suitable for embryo freezing according to claim 1, characterized in that: Handles (21) facilitating opening are arranged on the upper parts of the inner insulation cover (7) and the outer cover (6).

5. The liquid nitrogen box suitable for embryo freezing according to claim 1, characterized in that: A handle (22) is hingedly connected to the outer side of the outer protective shell (3).

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