Water bath constant-temperature incubator

Abstract

The utility model relates to the technical field of stem cell culture, in particular to a water bath constant-temperature incubator which comprises a heat preservation box, a sliding frame, a spline shaft, a missing gear, a fixing frame and a pushing mechanism. A heating assembly is arranged in the heat preservation box, and a box cover is arranged on the heat preservation box. The sliding frame is arranged in the heat preservation box, a supporting plate is arranged on the sliding frame, a rotating disc is rotationally arranged on the supporting plate, a plurality of containing holes are formed in the rotating disc, the rotating disc is coaxially connected with a fixing shaft, and the fixing shaft is coaxially connected with a bevel gear A; the spline shaft is rotationally connected with the heat preservation box and provided with a bevel gear B, and the bevel gear B is meshed with the bevel gear A; the missing gear is coaxially and rotationally connected with the fixed shaft; the fixing frame is arranged in the heat preservation box, two racks are oppositely arranged on the fixing frame, and the missing gear is alternately meshed with the racks on the two sides; the pushing mechanism is arranged in the heat preservation box. According to the utility model, the operation of oscillation and rotation transposition of the culture dish is completed through a group of driving components, and air can be prevented from entering the heat preservation box when the culture dish is taken and placed.

Description

TECHNICAL FIELD

[0001] The utility model relates to stem cell culture technical field especially relates to a water bath constant temperature incubator. BACKGROUND

[0002] Stem cells are a kind of cells with self-renewal and differentiation potential, which can proliferate and differentiate into multiple cell types in vitro environment. The core of stem cell culture technology is to promote the expansion and differentiation of stem cells through specific culture medium and growth factors to meet different research or treatment needs.

[0003] The Chinese patent with the authorization announcement number CN214973548U discloses a water bath temperature control constant temperature incubation oscillator, the device starts the heating pipe inside the editor starting frame, the heating pipe generates heat to heat the inside of the oscillator, the editor starts the fan at the upper end inside the oscillator, the rotation of the fan drives the heat inside the oscillator to flow from top to bottom, so that the heat forms a hot backflow in the oscillator, thereby making the solution evenly heated.

[0004] But the device still has some shortcomings: the device uses up-and-down air convection to make the solution evenly heated, but the heat conduction efficiency of air is low, so the uniform heating efficiency of water is low, and the device lacks a structure for separately taking and placing the culture dish, so external air can easily enter the inside. UTILITY MODEL CONTENT

[0005] The utility model aims at the problems in the background art and provides a water bath constant temperature incubator.

[0006] The technical scheme of the utility model: a water bath constant temperature incubator, including the heat preservation box, the heat preservation box is provided with heating assembly, the heat preservation box is provided with the box cover, the box cover is provided with the through -hole, and the box cover is provided with the screw cap for controlling the opening and closing of the through -hole;

[0007] The slide rail is slidably arranged in the heat preservation box, the support plate is arranged on the slide rail, the support plate is provided with a notch A on one side, the turntable is rotatably arranged on the support plate, a plurality of placing holes are arranged in an annular array around the shaft center of the turntable, a plurality of notches B corresponding to the placing holes are arranged on the turntable, the turntable is coaxially connected with the fixed shaft, the fixed shaft is rotatably connected with the slide rail, and the fixed shaft is coaxially connected with the bevel gear A;

[0008] The spline shaft penetrates through the slide rail and is rotatably connected with the heat preservation box, the heat preservation box is provided with motor A for driving the spline shaft to rotate, the spline shaft is coaxially provided with bevel gear B, and the bevel gear B is located in the slide rail and is engaged with the bevel gear A;

[0009] The gear with missing teeth is coaxially rotatably connected with the fixed shaft, and the fixed shaft is provided with a ratchet wheel assembly for limiting the one-way rotation of the gear with missing teeth;

[0010] The fixed frame is arranged in the heat preservation box, two racks are oppositely arranged on the fixed frame, and the missing gear is located between the two racks and alternately engages with the two racks.

[0011] And the pushing mechanism is arranged in the heat preservation box, and the pushing mechanism passes through the gap A and the gap B in the working state.

[0012] Preferably, a hinge is arranged on one side of the box cover, the movable end of the hinge is connected with the heat preservation box, a lock hook is arranged on the end of the box cover away from the hinge, a buckle matched with the lock hook is arranged on the heat preservation box, and a handle is arranged on the box cover.

[0013] Preferably, a perspective window is arranged on the heat preservation box, and a sliding cover for closing the perspective window is arranged on the heat preservation box in a sliding mode.

[0014] Preferably, the ratchet wheel assembly comprises a ratchet wheel and a ratchet tooth; a circular groove is coaxially arranged on the missing gear, the ratchet wheel is located in the circular groove and is coaxially connected with the fixed shaft, a sliding groove is arranged on the missing gear, a spring rod is arranged in the sliding groove, and one end of the ratchet tooth is inserted into the sliding groove and connected with the movable end of the spring rod.

[0015] Preferably, the pushing mechanism comprises a lifting driving assembly and a tray, the lifting driving assembly comprises a waterproof linear synchronous belt module; a sliding block is slidably connected with the inner wall of the heat preservation box, the tray is connected with the sliding block, the linear synchronous belt module is arranged in the heat preservation box, and the output end of the linear synchronous belt module is connected with the sliding block.

[0016] Preferably, the heating assembly comprises a heater, a temperature sensor and a controller, the heater and the temperature sensor are arranged in the heat preservation box and located below the liquid surface, the controller is arranged on the outer wall of the heat preservation box, and the temperature sensor and the heater are electrically connected with the controller.

[0017] Preferably, a tapered channel with a narrow upper part and a wide lower part is arranged in the heater, a water inlet hole communicated with the tapered channel is arranged at the bottom of the heater, a rotating shaft is coaxially arranged in the heater and rotationally connected with the heat preservation box, a spiral plate is coaxially arranged on the rotating shaft, a motor B is arranged on the heat preservation box, and the output end of the motor B is connected with the rotating shaft.

[0018] Compared with the prior art, the heat preservation box has the following beneficial technical effects:

[0019] By setting the box cover with through hole and the push mechanism, it is convenient to push a specified culture dish or test tube out of the through hole by the push mechanism, and the culture dish or test tube is operated alone, so that the temperature and the bacterial population environment in the incubator can be prevented from being out of control after external air enters the incubator; the rotating disc with a plurality of placing holes is arranged, and the fixed shaft, the bevel gear set and the missing gear and the rack are arranged in cooperation, so that the motor A can drive the rotating disc to rotate at the same point and also drive the rotating disc to rotate while reciprocating, so that the culture dish or test tube is shaken, and the function of the device is expanded; meanwhile, the spiral plate is arranged in the heater, the spiral plate is driven to rotate to circulate the water, so that the overall water heating efficiency is improved, and the water temperature of each point is consistent and constant. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 It is a structure schematic view of an embodiment of the utility model;

[0021] Figure 2 It is an internal structure schematic view of the incubator;

[0022] Figure 3 It is a connecting structure schematic view of each component on the sliding frame;

[0023] Figure 4 It is a connecting structure schematic view of the missing gear and the fixed frame;

[0024] Figure 5 It is a structure schematic view of the push mechanism;

[0025] Figure 6 It is a structure schematic view of the heating mechanism.

[0026] The figure sign: 1, the incubator; 2, box cover; 201, through hole; 3, screw cap; 4, sliding frame; 5, support plate; 51, notch A; 6, rotating disc; 61, placing hole; 62, notch B; 7, fixed shaft; 8, bevel gear A; 9, spline shaft; 10, bevel gear B; 11, motor A; 12, missing gear; 13, ratchet wheel; 14, ratchet; 15, fixed frame; 16, rack; 17, sliding block; 171, tray; 18, straight line synchronous belt module; 19, heater; 192, water inlet hole; 191, tapered channel; 20, spiral plate; 21, motor B. DETAILED DESCRIPTION

[0027] Embodiment one

[0028] As Figures 1-6The utility model provides a water bath constant temperature incubator, including heat preservation box 1, slide 4, spline shaft 9, missing gear 12, fixed frame 15 and push mechanism. The heat preservation box 1 is provided with heating assembly, is provided with box cover 2 on the heat preservation box 1, is provided with through -hole 201 on the box cover 2, and is provided with the screw cap 3 for controlling the opening and closing of through -hole 201 on the box cover 2. The hinge of the box cover 2 is provided with the movable end and is connected with the heat preservation box 1, and the lock hook is provided on the end away from the hinge of the box cover 2, the clasp that is provided with the lock hook is matched on the heat preservation box 1, and the handle is provided on the box cover 2. The heat preservation box 1 is provided with a perspective window, and the slide cover for closing the perspective window is slidably arranged on the heat preservation box 1. The heating assembly includes a heater 19, a temperature sensor and a controller, the heater 19 and the temperature sensor are arranged in the heat preservation box 1 and located below the liquid level, the controller is arranged on the outer wall of the heat preservation box 1, and the temperature sensor and the heater 19 are electrically connected with the controller. The slide 4 is slidably arranged in the heat preservation box 1, the support plate 5 is arranged on the slide 4, the notch A51 is arranged on one side of the support plate 5, the turntable 6 is rotatably arranged on the support plate 5, a plurality of placing holes 61 are arranged in an annular array around the axis of the turntable 6, a plurality of notches B62 corresponding to the placing holes 61 are arranged on the turntable 6, the fixed shaft 7 is coaxially connected with the turntable 6, the fixed shaft 7 is rotatably connected with the slide 4, and the bevel gear A8 is coaxially connected with the fixed shaft 7. The spline shaft 9 penetrates through the slide 4 and is rotatably connected with the heat preservation box 1, the motor A11 for driving the spline shaft 9 to rotate is arranged on the heat preservation box 1, the bevel gear B10 is coaxially arranged on the spline shaft 9, and the bevel gear B10 is located in the slide 4 and is engaged with the bevel gear A8. The missing gear 12 is coaxially rotatably connected with the fixed shaft 7, and the ratchet assembly for limiting the one-way rotation of the missing gear 12 is arranged on the fixed shaft 7. The ratchet assembly includes a ratchet wheel 13 and a ratchet 14. The missing gear 12 is coaxially provided with a circular groove, the ratchet wheel 13 is located in the circular groove and is coaxially connected with the fixed shaft 7, the missing gear 12 is provided with a sliding groove, a spring rod is arranged in the sliding groove, one end of the ratchet 14 is inserted into the sliding groove and is connected with the movable end of the spring rod. The fixed frame 15 is arranged in the heat preservation box 1, two racks 16 are oppositely arranged on the fixed frame 15, the missing gear 12 is located between the two racks 16 and is alternately engaged with the two racks 16. The push mechanism is arranged in the heat preservation box 1, and the push mechanism includes a lifting drive assembly and a tray 171. The lifting drive assembly includes a waterproof linear synchronous belt module 18. A sliding block 17 is slidably connected with the inner wall of the heat preservation box 1, the tray 171 is connected with the sliding block 17, the linear synchronous belt module 18 is arranged in the heat preservation box 1, the output end of the linear synchronous belt module 18 is connected with the sliding block 17, and the tray 171 passes through the notch A51 and the notch B62 in the working state of the push mechanism.

[0029] In this embodiment, first, a proper amount of sterile water is added into the incubator 1, the water level does not exceed the upper end surface of the support plate 5, the box cover 2 is started to open, the culture dishes are respectively placed into each placing groove 61, then the box cover 2 is closed, the motor A11 is started, the motor A11 drives the spline shaft 9 to rotate forward, thereby driving the fixed shaft 7 and the rotating disc 6 to rotate through the bevel gear A8 and the bevel gear B10, at the same time, the forward rotating fixed shaft 7 makes the ratchet wheel 13 engage with the ratchet teeth 14, thereby driving the cogwheel 12 to rotate, the cogwheel 12 alternately engages with the two side racks 16 in the rotating state, thereby making the sliding frame 4 reciprocate in the incubator 1, cooperating with the rotating rotating disc 6 to make the culture dishes (test tubes) oscillate in water; when it is needed to add nutrient agent into the culture dishes or test tubes, the sliding frame 4 is slid to the initial position, then the motor A11 drives the spline shaft 11 to rotate reversely, thereby making the rotating disc 6 rotate at the fixed position, each placing hole 61 passes through below the through hole 201 in turn, when the culture dishes or test tubes needing to add nutrient agent reach below the through hole 201, the linear synchronous belt module 18 is started, the linear synchronous belt module 18 drives the sliding block 17 to slide up and makes the tray 171 push the culture dishes or test tubes upward into the through hole 201, then the screw cover 3 is unscrewed to open the sealed cover and add nutrient agent.

[0030] Embodiment two

[0031] As shown in Figure 2 and Figure 6 the utility model discloses a water bath constant temperature incubator, compared to embodiment one, the taper channel 191 of upper narrow lower wide is arranged in the heater 19, and the water inlet hole 192 that communicates with the taper channel 191 is arranged at the bottom of the heater 19, the rotating shaft that rotates with the incubator 1 is arranged coaxially in the heater 19, the spiral plate 20 is arranged coaxially on the rotating shaft, the motor B21 is arranged on the incubator 1, and the output end of the motor B21 is connected with the rotating shaft.

[0032] In this embodiment, when heating water, the motor B21 is started, the motor B21 drives the rotating shaft and the spiral plate 20 to rotate, thereby pushing the hot water in the taper channel 191 upward and making it diffuse around along the upper end surface of the heater 19, at the same time, the surrounding low-temperature water enters the taper channel 191 through the water inlet hole 192 for rapid heating, which can make the water heat up quickly and uniformly, and keep the water temperature consistent at each point.

[0033] The embodiments of the utility model are described in detail above in combination with the drawings, but the utility model is not limited to this, within the knowledge scope of the technical personnel in the technical field to which the utility model belongs, various changes can be made without departing from the purpose of the utility model.

Claims

1. A water bath incubator characterized by, include A heat preservation box (1) is provided inside the heat preservation box (1). A box cover (2) is provided on the heat preservation box (1). A through hole (201) is provided on the box cover (2), and a screw cap (3) for controlling the opening and closing of the through hole (201) is provided on the box cover (2). The slide (4) is slidably set inside the heat preservation box (1). A support plate (5) is set on the slide (4). A notch A (51) is set on one side of the support plate (5). A turntable (6) is rotatably set on the support plate (5). Several placement holes (61) are arranged in a ring array around its axis on the turntable (6). Several notches B (62) are set on the turntable (6) that correspond one-to-one with each placement hole (61). The turntable (6) is coaxially connected to a fixed shaft (7). The fixed shaft (7) is rotatably connected to the slide (4). The fixed shaft (7) is coaxially connected to a bevel gear A (8). A spline shaft (9) passes through the slide (4) and is rotatably connected to the insulation box (1). A motor A (11) is installed on the insulation box (1) to drive the spline shaft (9) to rotate. A bevel gear B (10) is coaxially installed on the spline shaft (9). The bevel gear B (10) is located inside the slide (4) and meshes with the bevel gear A (8). A missing gear (12) is coaxially rotatably connected to a fixed shaft (7), and a ratchet assembly is provided on the fixed shaft (7) to limit the unidirectional rotation of the missing gear (12); A fixed frame (15) is set inside the heat preservation box (1). Two racks (16) are arranged opposite each other on the fixed frame (15). A missing gear (12) is located between the two racks (16) and meshes alternately with the two racks (16). And a jacking mechanism, which is set inside the insulation box (1), and the jacking mechanism passes through the gap A (51) and the gap B (62) in the working state.

2. The water bath constant temperature incubator according to claim 1, characterized in that, A hinge is provided on one side of the lid (2), and the movable end of the hinge is connected to the insulated box (1). A locking hook is provided on the lid (2) at the end away from the hinge. A buckle that cooperates with the locking hook is provided on the insulated box (1), and a handle is provided on the lid (2).

3. The water bath constant temperature incubator according to claim 1, characterized in that, A viewing window is provided on the insulated box (1), and a sliding cover for closing the viewing window is slidably provided on the insulated box (1).

4. The water bath constant-temperature incubator according to claim 1, characterized in that, The ratchet assembly includes a ratchet (13) and a ratchet tooth (14); a circular groove is coaxially provided on the gear (12), the ratchet (13) is located in the circular groove and coaxially connected to the fixed shaft (7), a sliding groove is provided on the gear (12), a spring rod is provided in the sliding groove, one end of the ratchet tooth (14) is inserted into the sliding groove and connected to the movable end of the spring rod.

5. The water bath constant-temperature incubator according to claim 1, characterized in that, The jacking mechanism includes a lifting drive assembly and a tray (171). The lifting drive assembly includes a waterproof linear synchronous belt module (18). A slider (17) is provided inside the insulation box (1) and is slidably connected to its inner wall. The tray (171) is connected to the slider (17). The linear synchronous belt module (18) is provided inside the insulation box (1). The output end of the linear synchronous belt module (18) is connected to the slider (17).

6. The water bath constant-temperature incubator according to claim 1, characterized in that, The heating assembly comprises a heater (19), a temperature sensor and a controller, the heater (19) and the temperature sensor are arranged in the heat preservation box (1) and below the liquid level, the controller is arranged on the outer wall of the heat preservation box (1), and the temperature sensor and the heater (19) are electrically connected with the controller.

7. A water bath constant-temperature incubator according to claim 6, characterized in that, The heater (19) is provided with a tapered channel (191) which is narrow at the top and wide at the bottom, and the bottom of the heater (19) is provided with a water inlet hole (192) communicated with the tapered channel (191), a rotating shaft which is rotatably connected with the heat preservation box (1) is coaxially arranged in the heater (19), a spiral plate (20) is coaxially arranged on the rotating shaft, a motor B (21) is arranged on the heat preservation box (1), and an output end of the motor B (21) is connected with the rotating shaft.

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