Pathological slide dyeing device based on advanced fluid control technology
By integrating centrifugation and slide preparation/staining components with advanced fluid control technology, the problems of cumbersome and unstable traditional pathological slide preparation and staining equipment have been solved, achieving fully automated processing, improving efficiency and reducing costs, and ensuring the accuracy of experimental results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2026-04-10
AI Technical Summary
Traditional pathological slide preparation and staining equipment is cumbersome, unstable, requires multiple devices, has high manufacturing and operating costs, low efficiency, and the fluid control method is prone to causing experimental errors.
Employing advanced fluid control technology, the centrifugation and slide preparation/staining components are integrated into one unit to achieve fully automated processing, simplifying operation steps, reducing the number of devices and space occupation. The liquid circuit system designed with advanced fluid control technology improves liquid transfer efficiency and stability.
It achieves a high degree of integration and automation in pathological slide preparation and staining, improves experimental efficiency, reduces operational complexity and equipment costs, reduces failure rate, and ensures the accuracy and consistency of experimental results.
Smart Images
Figure CN224109153U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to pathological section dyeing automation field, concretely is a cytological section dyeing and histological immunohistochemistry and special dyeing automation device based on advanced fluid control technology. BACKGROUND
[0002] Clinical pathology can be divided into histopathology and cytopathology, the most commonly used sample processing method for histology is paraffin section; centrifugal sedimentation type section and separate drop dyeing are ideal sample pretreatment methods for cytology; histopathology and cytopathology staining methods include traditional chemical staining, such as HE staining, bismuth staining, etc.; and special staining, such as immunohistochemistry and special staining, etc. However, traditional manual and various semi-automatic section dyeing processes developed later are complicated and inefficient. Most of the semi-automatic equipment on the market can only realize single histology or cytology or tissue chip sectioning or a certain staining function, and cannot complete multiple functions and full-process automation at the same time. Among them, the traditional sedimentation type liquid-based cell section dyeing semi-automatic process usually involves multiple independent steps, including sample oscillation, transfer, centrifugation and section dyeing, and requires manual sample transfer, equipment transfer and marking multiple times, and the working process is not only complicated and time-consuming, but also prone to human error, resulting in experimental errors.
[0003] In addition, the traditional method also needs to be equipped with multiple instruments, such as oscillation transfer machine, centrifuge, section dyeing machine, immunohistochemical staining machine, etc., which not only increases the equipment cost, but also occupies a large amount of laboratory space, and the equipment failure rate is high. Therefore, there is an urgent need in the market for a device and method that can integrate and automate the whole process of cytology and histology section dyeing, and for large laboratories, a high-throughput assembly line is also needed to improve experimental efficiency, reduce operation complexity and cost, reduce equipment space occupation, and reduce failure rate.
[0004] The traditional fluid control method is used in in vitro diagnosis applications, such as transferring sample liquid in one container to another container, usually with the help of an intermediate carrier, such as a liquid suction gun and a disposable suction head. Because of the need for repeated high-frequency operation, the one-time suction head has a work difference, etc., it is easy to cause sample suction, leakage, inaccurate quantification, etc., resulting in experimental errors.
[0005] Traditional fluid control methods for pathological slide preparation and staining rely on three-axis robotic arms to move pipette tips, needles, and tubing. However, this multi-directional mechanical movement leads to inaccurate positioning, requiring cleaning of components or replacement of disposable consumables like pipette tips after each use to prevent contamination. A single mechanical system can only perform one liquid transfer or one functional action at a time, such as centrifugation, shaking, or pipetting. Integrating a shaking module into an integrated instrument typically involves ultrasonic vibration motors, increasing instrument cost and causing cell sample deformation that can affect diagnosis, resulting in low efficiency. Furthermore, long paths and numerous bends in the tubing can lead to leaks and air leaks, causing errors in liquid addition and aspiration. Therefore, traditional fluid control instruments require frequent maintenance and calibration to ensure stable and reliable operation. However, advanced fluid control methods and technologies can effectively solve these problems. Utility Model Content
[0006] The purpose of this invention is to provide a pathological slide preparation and staining device based on advanced fluid control technology, in order to solve the problems mentioned in the background art, such as the cumbersome and unstable nature of traditional manual and various semi-automatic slide preparation and staining devices, the need for multiple devices, high manufacturing and usage costs, and low efficiency.
[0007] To achieve the above objectives, this utility model provides an automated device for pathological slide preparation and staining based on advanced fluid control technology. Its features include: a single integrated, fully automated system for centrifugation-sedimentation cytology sample pretreatment and slide preparation / staining; optional installation and replacement of consumables on the same device to switch between cytology, histology, tissue microarray, and high-throughput automated line configurations; and adaptation to special staining requirements such as immunohistochemistry. Its structure includes an upper centrifugation assembly and a lower slide preparation / staining assembly. The centrifugation assembly includes a centrifugation drive component and an upper centrifugation fluid path component. The slide preparation / staining assembly includes an arc-shaped staining turntable and an upper staining fluid path component. Several slide preparation / staining units and several waste liquid tanks are arranged on the staining turntable. The liquid after centrifugation is directly poured into the slide preparation / staining assembly or the waste liquid tank below.
[0008] Preferably, a plurality of centrifugal units are annularly mounted on the outer side of the centrifugal drive component, and each centrifugal unit includes a basket, the inside of which is provided with a plurality of centrifugal test tubes.
[0009] Preferably, the upper centrifuge assembly and the lower slide preparation and staining assembly can be controlled by an external motor to move and rotate relative to each other. A lifting plate is installed on the staining turntable, and the lifting plate is located below the centrifuge unit. The lifting plate lifts the centrifuge unit, so that the positions of the slide preparation and staining unit or waste liquid tank and the centrifuge unit correspond one-to-one through positioning, and the centrifuge unit is flipped to directly pour and transfer the liquid in the centrifuge tube into the slide preparation and staining unit or waste liquid tank.
[0010] As a preferred, the centrifugal tube can be used as a cell sample preservation liquid bottle at the same time; a sleeve is attached to the inner wall of the centrifugal tube, the lower end of the sleeve is close to the bottom of the centrifugal tube, and a filter screen is installed, the filter screen is close to the bottom of the centrifugal tube, a sampling brush or an alternative stirring object is placed outside the sleeve, the sampling brush tip or the alternative stirring object is close to the position of the bottom of the centrifugal tube, a vibration motor is installed below the centrifugal tube, and the two cooperate to shake and disperse the cell sample more fully.
[0011] As a preferred, the centrifugal liquid path component includes a centrifugal tube liquid adding needle, a driving pump, and a matched pipeline and a reagent bottle; the centrifugal tube liquid adding needle is fixed above the centrifugal unit, and the corresponding liquid adding position is positioned by rotating the centrifugal driving component through a centrifugal positioning motor.
[0012] As a preferred, the liquid path component of the slide preparation and staining assembly is divided into two types: one is a liquid path component for traditional cytological staining, such as for Papanicolaou staining and HE staining; and the other is a liquid path component for immunohistochemistry and special staining (including tissue chip) of cytology and histology.
[0013] As a preferred, the traditional staining liquid path component includes a plurality of liquid adding needles, a plurality of waste liquid suction needles, a matched pipeline, a driving pump, and a reagent bottle / reservoir bottle, a slide preparation and staining unit, and a slide preparation chamber special for traditional staining.
[0014] As a preferred, the immunohistochemistry and special staining liquid path component includes a plurality of immunohistochemistry and special staining liquid adding needles, a plurality of immunohistochemistry and special staining liquid suction needles, a plurality of special antibody reagent bottles, a plurality of single-head precise liquid adding needles, and a plurality of multi-head precise liquid adding needles; the slide preparation and staining unit includes a special staining special slide, a slide preparation chamber-distributor, a matched pipeline, a driving pump, and a reagent bottle.
[0015] As a preferred, the special staining special slide is a slide with a hydrophobic coating separating a plurality of slide preparation adhesion areas. The slide preparation chamber-distributor is arranged on the upper part of the special staining special slide, the upper layer of which is provided with a liquid storage pool, an overflow outlet, and a plurality of flow channel grooves, the thickness and length of the flow channel grooves are designed according to the flow rate, the end of the flow channel groove has a downward channel, liquid can be dropped onto the slide preparation hole of the slide, and the excess liquid is discharged through the overflow groove to ensure the consistency of the liquid amount each time. In addition, the liquid suction needle is aligned with the channel to suck the waste liquid on the corresponding slide preparation hole and the slide preparation chamber-distributor; the lower end of the drop channel is close to the slide preparation area, which plays a role in evenly dividing the liquid amount of multiple holes when dropping liquid; the bottom of the slide preparation chamber-distributor is provided with a plurality of steam holes, and the lower part of the slide preparation chamber-distributor is provided with a water bath box. The adjustable heating liquid generates steam, which enters the upper part of the slide through the steam holes in the lower part of the slide preparation chamber-distributor, and plays a role in keeping the sample warm and humid.
[0016] As preferred, a piston is arranged on the reagent bottle of the immunohistochemistry and special staining dedicated liquid path system, which is used as a syringe pump; the reagent bottle is provided with a single-head or multi-head precise liquid adding needle, and the immunohistochemistry and special staining dedicated liquid path system adopts a multi-head immunohistochemistry and special staining liquid suction needle.
[0017] Compared with the prior art, the utility model has the beneficial effects that:
[0018] 1. In the pathological section dyeing device based on advanced fluid control technology, the high integration and automation of the section dyeing process are realized. By integrating the centrifugal component, the turntable, the centrifugal unit, the section dyeing plate and the pipetting component and other key components, the utility model can complete the whole process from sample preparation, centrifugal treatment to section dyeing on the same equipment, greatly simplifying the cumbersome steps of the traditional section dyeing process. This not only significantly improves the experimental efficiency and reduces the operation complexity, but also reduces the equipment cost and the laboratory space occupation, and reduces the equipment failure rate. The device can be optionally provided with different components and consumables to present cytology, histology staining, immunohistochemistry and special staining, tissue chip staining and high-throughput flow line forms to meet different section and dyeing functions and improve the diversity of equipment functions.
[0019] 2. In the pathological section dyeing device based on advanced fluid control technology, the centrifugal test tube is used as a cell sample storage liquid bottle; the gradient density centrifugation and the coarse impurity filtering steps are performed synchronously; and before centrifugation, the cell sample does not need to be pre-shaken and is directly put into the machine; after centrifugation, the sample is dispersed by a mechanical vibration motor, which has a lower cost than an ultrasonic motor and will not cause cell deformation to affect the staining and morphological effect. The immunohistochemistry and special staining are performed to make a slide (tissue / cell chip) with multiple section areas by one-step sample adding. The above-mentioned simplification of the use process improves the efficiency and reduces the cost.
[0020] 3. In the pathological section dyeing device based on advanced fluid control technology, the centrifuge motor has the positioning function during liquid adding in addition to the centrifugal effect, and the centrifugation and positioning are completed in one step. The centrifuge motor can be installed above the centrifugal driving member, further reducing the space of the equipment, and reducing the volume, complexity and production cost of the equipment.
[0021] 4. In the pathological section dyeing device based on advanced fluid control technology, the liquid adding device pipeline is designed based on advanced fluid control technology, greatly shortening the liquid path stroke, improving the efficiency by using one peristaltic pump to drive multiple liquid paths; without the commonly used three-axis mechanical arm and liquid transfer consumables such as a suction head, the liquid can be directly poured or dripped into the section warehouse, multiple liquid paths are simultaneously actuated, the complexity of mechanical action is greatly simplified, the accuracy requirements of the motor and the liquid pump are reduced, the user can conveniently replace the hose, the efficiency is doubled, the efficiency and stability of the equipment are improved, and the maintenance cost and use cost of the equipment are reduced.
[0022] 5、The pipeline form of pathological section dyeing device based on advanced fluid control technology, the section dyeing assembly is a large annular pipeline structure, and the section dyeing unit can be replaced up and down without stopping the centrifugal driving member. The use flux of the equipment is greatly improved, and a scanning and printing slide bar code function module, a subsequent sealing and scanning function module and the like can be additionally installed, so that the flux and the function are further expanded. BRIEF DESCRIPTION OF DRAWINGS
[0023] Figure 1 It is an internal overall structure schematic view of the utility model;
[0024] Figure 2 It is a schematic view of the centrifugal assembly structure and motor installation in the utility model;
[0025] Figure 3 It is a schematic view of the structure of the section dyeing assembly of traditional dyeing in the utility model plus lifting plate;
[0026] Figure 4 It is a schematic view of the structure of the section dyeing assembly of immunohistochemistry and special dyeing in the utility model;
[0027] Figure 5 It is a schematic view of the internal structure of the centrifugal test tube in the utility model;
[0028] Figure 6 It is a schematic view of the effect that the centrifugal test tube is lifted and pours and transfers liquid to the section warehouse in the utility model;
[0029] Figure 7 It is a schematic view of the effect that the sample gradient centrifugal separation and impurity pouring in the utility model;
[0030] Figure 8 It is a schematic view of the effect that the sample oscillation scattering and transfer in the utility model;
[0031] Figure 9 It is a schematic view of the structure of the section warehouse-distributor and slide and the sample liquid filling in the utility model;
[0032] Figure 10 It is a schematic view of the structure section of the section warehouse-distributor and the liquid filling and liquid suction in the utility model;
[0033] Figure 11 It is a schematic view of the pipeline form in the utility model
[0034] The meanings of various reference numerals in the drawing are as follows:
[0035] 1, centrifugal assembly; 11, centrifugal driving member; 101, centrifugal liquid path member; 102, centrifugal test tube liquid adding needle; 2, slice making and staining assembly; 21, staining turntable; 22, waste liquid tank; 23, lifting plate; 201, traditional staining liquid path member; 202, immunohistochemical and special staining liquid path member; 2021, immunohistochemical and special staining liquid adding needle; 2022, immunohistochemical and special staining liquid sucking needle; 2023, reagent bottle; 2024, single-head precise liquid adding needle; 2025, multi-head precise liquid adding needle; 2026, piston; 3, centrifugal unit; 31, hanging basket; 32, centrifugal test tube; 321, sleeve; 322, filter screen; 323, sampling brush; 324, gradient density separation liquid; 325, sample cell; 326, upper layer impurity; 327, cell suspension; 328, vibration motor; 329, dilution liquid; 4, slice making and staining unit; 5, slide; 51, traditional staining slide; 52, special staining special slide; 53, slice making area; 6, slice making bin; 61, traditional staining special slice making bin; 62, slice making bin-liquid separator; 621, liquid storage pool; 622, overflow outlet; 623, flow channel groove; 624, hole channel; 625, steam hole; 626, water bath box; 7, centrifugal positioning motor; 8, staining positioning motor; 9, lifting motor. DETAILED DESCRIPTION
[0036] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.
[0037] The present application provides a pathological slice making and staining device based on advanced fluid control technology, which adopts upper and lower layered design, such as Figures 1-11As shown, including the upper centrifugal assembly 1 and the lower sheet dyeing assembly 2, the centrifugal assembly 1 includes a centrifugal drive member 11 and an upper centrifugal liquid path member, the sheet dyeing assembly 2 includes a circular arc dyeing turntable 21 and an upper dyeing liquid path member; the dyeing turntable 21 is arranged with a plurality of sheet dyeing units 4 and a plurality of waste liquid tanks 22; the liquid after the centrifugal assembly 1 completes centrifugation is directly poured and transferred to the sheet dyeing assembly 2 or the waste liquid tank 22 below. Through advanced fluid control technology, the centrifugal assembly and the sheet dyeing assembly are ingeniously combined together, and accurate control and efficient transfer of the liquid are realized. This layout not only saves space, but also makes the whole device more compact and reasonable. At the same time, the circular arc dyeing turntable design facilitates the arrangement and mechanical action of the sheet dyeing unit and the waste liquid tank, and improves the work efficiency. This design can complete multiple liquid transfer operations in one action; after the centrifugal assembly completes the centrifugal operation, the liquid can be directly poured and transferred to the sheet dyeing assembly or the waste liquid tank, which simplifies the multiple transfer and operation steps in the traditional sheet dyeing process, improves the work efficiency, reduces the use of consumables, reduces the use cost, and also helps to improve the consistency and quality of the sheet.
[0038] The device adopts upper and lower layered design, the centrifugal assembly is located in the upper part, and the sheet dyeing assembly is located in the lower part. This layout not only saves space, but also makes the whole device more compact and reasonable. At the same time, the circular arc dyeing turntable design facilitates the arrangement and mechanical action of the sheet dyeing unit and the waste liquid tank, and improves the work efficiency.
[0039] A plurality of waste liquid tanks are arranged on the dyeing turntable, so that a plurality of centrifuged waste liquids can be directly poured to the specified position, facilitating the collection and treatment of the waste liquid.
[0040] In this embodiment, a plurality of centrifugal units 3 are annularly mounted outside the centrifugal drive member 11, and the centrifugal unit 3 includes a hanging basket 31, and a plurality of centrifugal test tubes 32 are arranged in the inside of the hanging basket 31.
[0041] Specifically, the upper centrifugal assembly 1 and the lower slice dyeing assembly 2 can be controlled to move up and down and rotate by external motors, including a centrifugal positioning motor 7, a dyeing positioning motor 8, and a lifting motor 9. The dyeing positioning motor 8 controls the rotation positioning, so that the slice dyeing unit 4 or the waste liquid tank 22 and the centrifugal unit 3 are positioned one by one in correspondence with the up and down positioning. The dyeing turntable 21 is provided with a lifting plate 23, which is located below the centrifugal unit 3. The centrifugal assembly 1 and the slice dyeing assembly 2 move up and down relative to each other, so that the lifting plate 23 lifts the centrifugal unit 3, and the centrifugal unit 3 is turned over to directly pour the liquid in the centrifugal test tube into the slice dyeing unit 4 or the waste liquid tank 22. The liquid in the centrifugal driving assembly 1 is directly poured into the slice dyeing assembly 2 or the waste liquid tank 22 below after centrifugation. In addition to the function of centrifuging the sample, the centrifugal driving assembly 1 also has the function of shaking or vibrating the motor 328 to disperse the sample.
[0042] Further, the centrifugal test tube 32 can also serve as a cell sample preservation liquid bottle. A sleeve 321 is attached to the inner wall of the centrifugal test tube 32. The lower end of the sleeve 321 is close to the bottom of the centrifugal test tube 32 and is provided with a filter screen 322 close to the bottom of the centrifugal test tube 32. During sampling, the sampling brush is placed outside the sleeve 321, and the sampling brush 323 or the alternative stirring material (such as a suitable size suction head) is directed at the position of the bottom of the centrifugal test tube. A vibration motor 328 is installed below the centrifugal test tube 32, and the two cooperate to shake and stir, better dispersing the cell sample to make it suspended. Before centrifugation, a gradient density separation liquid is slowly added from the sleeve 321 into the bottom of the centrifugal test tube 32, so that most of the suspended cells are in the upper part of the separation liquid. After centrifugation and shaking, a diluent or buffer is added to fully suspend and evenly distribute the cell sample.
[0043] Further, the centrifugal liquid path component includes a centrifugal test tube liquid adding needle, a driving pump, and a matching pipeline and a reagent bottle. The centrifugal test tube liquid adding needle is fixed above the centrifugal unit 3, and the centrifugal driving component 11 is rotated by the centrifugal positioning motor 7 to position the corresponding liquid adding position.
[0044] Further, the liquid path component of the slice dyeing assembly 2 is divided into two types: one is used for traditional dyeing, such as for Papanicolaou staining and HE staining; and the other is used for immunohistochemistry and special staining.
[0045] Further, the slice dyeing unit 4 includes a slice warehouse 6, and each of the warehouses is provided with a slide 5. The slice warehouse 6 is divided into two types, i.e., a slice warehouse 61 special for traditional dyeing and a special slice warehouse 62 special for immunohistochemistry and special staining. The slides are divided into two categories: traditional dyeing slides 51 and special dyeing slides 52 special for immunohistochemistry.
[0046] Further, the traditional dyeing liquid path component 201 comprises a plurality of liquid adding needles, a plurality of waste liquid suction needles, matched pipelines and driving pumps and reagent bottles / reservoir bottles, a slice dyeing unit 4 and a traditional dyeing special slice making chamber 61.
[0047] Further, the immunohistochemical and special dyeing special liquid path component 202 comprises a plurality of special antibody reagent bottles 2023, single-head precise liquid adding needles 2024 and multi-head precise liquid adding needles 2025, a plurality of immunohistochemical and special dyeing liquid suction needles 2022, a plurality of liquid adding needles 2021, other matched pipelines and driving pumps and reagent bottles / reservoir bottles, and a piston 2026 arranged in the antibody reagent bottle 2023 and used as an injection pump. The slice dyeing unit 4 comprises special dyeing special slides 52, a slice making chamber-liquid distributor 62, matched pipelines and driving pumps and reagent bottles.
[0048] Further, the liquid adding driving pump of the centrifugal liquid path component 101 and the liquid adding driving pump and the waste liquid suction driving pump of the traditional dyeing liquid path component 201 are all sample quantitative peristaltic pumps, which are lower in cost and convenient for users to replace hoses.
[0049] Further, the immunohistochemical and special dyeing special slide 52 has a hydrophobic coating layer for separating a plurality of slice making adhesion areas 53. The slice making chamber-liquid distributor 62 is provided with a liquid storage pool 621, an overflow outlet 622 and a plurality of flow channel grooves 623 in the upper layer, the flow channel grooves 623 are provided with different thicknesses and lengths according to flow rates, the flow channel grooves 623 are provided with downward hole channels 624 at the ends, the downward hole channels 624 are tightly arranged opposite to the slice making areas 53, and the downward hole channels 624 have the function of evenly distributing liquid through multiple holes. The slice making chamber-liquid distributor 62 is provided with a plurality of steam holes 625 at the bottom, and a water bath box 626 is arranged below the slice making chamber-liquid distributor 62. The water temperature can be controlled and set, and the water temperature can be conducted to the immunohistochemical and special dyeing special slide 52 and the sample. The steam in the water bath box can enter the inside of the slice making chamber-liquid distributor 62 through the steam holes in the bottom of the slice making chamber-liquid distributor 62, so as to play a role of heat preservation and moisture preservation for the sample.
[0050] Further, the slice dyeing component 2 of the utility model can be designed into a large annular assembly line structure, more slice dyeing units 4 can be installed, the slice dyeing units 4 can be replaced up and down without stopping the centrifugation, the throughput and functions can be further expanded, the scanning and printing slide bar code function can be additionally installed, and the subsequent sealing and scanning functions are also provided.
[0051] The pathological slice dyeing device based on the advanced fluid control technology comprises the following steps in use:
[0052] 1. Take the cell slice making and traditional dyeing - single machine scheme as an example:
[0053] S1, sample and slide preparation: when sampling, the sampling brush is placed outside the sleeve, the sampling brush tip or the alternative stirrer is directed at the sharp position at the bottom of the centrifugal test tube, the cell preservation liquid bottle / centrifugal test tube is manually placed, the filter screen is installed, the slide and the slide making bin are installed at the specified position;
[0054] S2, cell sample shaking and centrifugation: the centrifugal test tube is shaken by rotating the centrifugal drive member back and forth to disperse the cells in the tube; the separation liquid is added through the liquid adding device above, slowly from the bottom of the centrifugal test tube, and the centrifugal positioning motor is started to centrifuge;
[0055] S3, sample transfer and sedimentation slide making: the slide dyeing plate is lifted to pour the upper centrifugal waste liquid into the waste liquid tank; the dilution liquid is added to the centrifugal test tube through the liquid adding device above, and the vibration motor is started to shake the centrifugal test tube, cooperating with the cervical brush or the stirrer, to disperse the sample into a cell suspension state;
[0056] S4, sedimentation slide making: the cell suspension sample in the centrifugal test tube is poured into the slide making bin below by lifting the slide dyeing plate, and is left for a certain period of time to allow the cells to settle and adhere to the slide;
[0057] S5, traditional staining of cell slides (Papanicolaou staining or HE staining, etc.): the waste liquid is sucked away by the waste liquid suction needle, the staining liquid and the washing liquid are added according to the requirements of the reagent instruction manual, and the waste liquid is finally sucked away. The slide will be kept moist to prevent dry slides;
[0058] S6, the dyed slide is taken out of the slide making bin for subsequent mounting, scanning or observation, etc.
[0059] 2. Cell making immunohistochemical staining - single machine scheme for example:
[0060] S1, sample and slide preparation: when sampling, the sampling brush is placed outside the sleeve, the sampling brush tip or the alternative stirrer is directed at the sharp position at the bottom of the centrifugal test tube, the cell preservation liquid bottle / centrifugal test tube is manually placed, the filter screen is installed, the slide and the appropriate immunohistochemical special slide making bin-distributor are installed at the specified position;
[0061] S2-S4, the steps are consistent with the above 1;
[0062] S5, immunohistochemical staining of cell slides: the water bath box is pre-opened, and the temperature is set according to the requirements of the reagent instruction manual; the waste liquid on the slide is sucked away by the waste liquid suction needle; the program is set according to the requirements of the reagent instruction manual, such as: adding primary antibody reagent with reagent bottle and single-head precise liquid adding needle, constant temperature and humidity incubation, adding washing liquid and sucking waste liquid with multi-head liquid suction needle, adding secondary antibody reagent with reagent bottle and multi-head precise liquid adding needle, constant temperature and humidity incubation, adding washing liquid and sucking waste liquid, and finally the slide will be kept moist to prevent dry slides;
[0063] S6, the dyed slide is taken out from the slide preparation bin-liquid distributor, and subsequent processes such as mounting, scanning or observation are performed.
[0064] 3. Histological immunohistochemical staining (single machine program is taken as an example):
[0065] S1, a slide is manually prepared according to an experimental procedure: for example, sample dehydration, embedding, slicing, slide floating, slide fishing, slide baking, dewaxing, water, antigen repair and the like. The slide and the slide fishing should be noted: the adhesive anti-removal slide should be used, the slide has a waterproof coating and is separated into several areas; the tissue slice sample position must fall in the adhesive area in the waterproof isolation zone of the slide during the slide fishing operation.
[0066] S2-S6, the steps corresponding to the above 2 are consistent.
[0067] Finally, it should be noted that the centrifugal positioning motor 7 and the electronic components in the above components in the embodiment are all general standard components or components known to those skilled in the art, and their structure and principle are known to those skilled in the art through technical manuals or through conventional experimental methods. In the idle place of the device, all the electrical components are connected through wires respectively, and the specific connection means should be completed according to the working order of the electrical components in the above working principle, which are all known technologies in the art. In the centrifugal liquid path component, the connecting centrifugal test tube liquid adding needle, three-way pipe fitting and reagent bottle / liquid storage bottle are included; in the staining liquid path component, the connecting liquid adding needle, liquid suction needle, staining needle, reagent bottle / liquid storage bottle and waste liquid bottle are included, all the above components are general standard components or components known to those skilled in the art, and their structure and principle are known to those skilled in the art through technical manuals or through conventional experimental methods. In the idle place of the device, all the components are connected through pipes respectively, and the specific connection means should be completed according to the working order of the components in the above working principle, which are all known technologies in the art.
[0068] The basic principle, main features and advantages of the present application are shown and described above. Those skilled in the art should understand that the present application is not limited by the above embodiments, and the above embodiments and descriptions in the specification are only preferred examples of the present application and do not limit the present application. Without departing from the spirit and scope of the present application, the present application can also have various changes and improvements, and these changes and improvements all fall within the scope of the claimed present application.
Claims
1. An advanced fluid control technology based histopathological slide staining apparatus comprising an upper centrifugation assembly (1) and a lower slide staining assembly (2), characterized in that: The centrifugal assembly (1) comprises a centrifugal driving member (11) and an upper centrifugal liquid path member (101), and the slice dyeing assembly (2) comprises a circular arc-shaped dyeing turntable (21) and an upper dyeing liquid path member; a plurality of slice dyeing units (4) and a plurality of waste liquid tanks (22) are arranged on the dyeing turntable (21); the liquid after the centrifugal assembly (1) is completed is directly poured and transferred to the slice dyeing assembly (2) or the waste liquid tank (22) below; A plurality of centrifugal units (3) are annularly mounted on the outside of the centrifugal driving member (11), and the centrifugal unit (3) comprises a hanging basket (31), and a plurality of centrifugal test tubes (32) are arranged in the inside of the hanging basket (31). The upper centrifugal assembly (1) and the lower slice dyeing assembly (2) can be relatively moved up and down and rotationally positioned by an external motor, so that the slice dyeing unit (4) or the waste liquid tank (22) and the centrifugal unit (3) are one-to-one corresponding in position; the dyeing turntable (21) is provided with a lifting plate (23) and is located below the centrifugal unit (3); the centrifugal unit (3) is lifted by the lifting plate (23) through the relative movement up and down, and the centrifugal unit (3) is turned over to directly pour and transfer the liquid in the centrifugal test tube (32) to the slice dyeing unit (4) or the waste liquid tank (22).
2. The advanced fluid control technology based histopathological slide staining device as claimed in claim 1, wherein: The centrifugal test tube (32) can be used as a cell sample storage liquid bottle at the same time; a sleeve (321) is arranged close to the inner wall of the centrifugal test tube (32), the lower end outlet of the sleeve (321) is close to the bottom of the centrifugal test tube (32) and is provided with a filter screen (322), the filter screen (322) is close to the bottom of the centrifugal test tube (32), a sampling brush (323) or a replaced stirring object is arranged outside the sleeve (321), and the tip of the sampling brush (323) or the replaced stirring object is close to the position of the bottom of the centrifugal test tube; a vibration motor (328) is installed below the centrifugal test tube (32).
3. The advanced fluid control technology based histopathological slide staining device as claimed in claim 1, wherein: The centrifugal liquid path member (101) comprises a centrifugal test tube liquid adding needle (102), a driving pump, a matched pipeline and a reagent bottle, and a centrifugal positioning motor (7); the centrifugal test tube liquid adding needle (102) is fixed above the centrifugal unit (3), and the centrifugal positioning motor (7) is installed below or above the centrifugal driving member (11) to rotate the centrifugal driving member (11) to position the corresponding liquid adding position.
4. The advanced fluid control technology based histopathological slide staining device as claimed in claim 1, wherein: The dyeing liquid path member of the slice dyeing assembly (2) is divided into two kinds: one is a traditional dyeing liquid path member (201), which is used for the Papanicolaou staining and HE staining; and the other is an immunohistochemical and special dyeing liquid path member (202).
5. The advanced fluid control technology based histopathological slide staining device as claimed in claim 4, wherein: The traditional dyeing liquid path member (201) comprises a plurality of liquid adding needles, a plurality of waste liquid suction needles, a matched pipeline, a driving pump, a reagent bottle / storage bottle, a slice dyeing unit (4) and a slice dyeing special slice warehouse (61).
6. The advanced fluid control technology based histopathological slide staining device according to any one of claims 4 or 5, wherein: The immunohistochemistry and special staining liquid path component (202) comprises a plurality of immunohistochemistry and special staining liquid adding needles (2021), a plurality of immunohistochemistry and special staining liquid sucking needles (2022), a plurality of special antibody reagent bottles (2023), a plurality of single-head precise liquid adding needles (2024) and a plurality of multi-head precise liquid adding needles (2025); the sectioning and staining unit (4) comprises special staining special slides (52), a sectioning and separating device (62), matched pipelines and a driving pump and reagent bottles.
7. The advanced fluid control technology based histopathological slide staining device as claimed in claim 6, wherein: The sectioning and separating device (62) functions to divide the liquid for the multi-sectioning area (53) when dripping the liquid, is arranged on the upper part of the special staining special slide (52), and is provided with a liquid storage pool (621), an overflow outlet (622) and a plurality of flow channel grooves (623) on the upper layer; the flow channel grooves (623) are set in thickness and length according to the required flow, and the end of the flow channel grooves (623) is provided with downward dripping channels (624) which are closely arranged below the sectioning area (53).
8. The advanced fluid control technology based histopathological slide staining device as claimed in claim 6, wherein: The reagent bottle (2023) of the immunohistochemistry and special staining special liquid path system is provided with a piston (2026) which is also used as an injection pump; the reagent bottle is provided with the single-head precise liquid adding needle (2024) or the multi-head precise liquid adding needle (2025).