Detection image acquisition device

Abstract

In the detection image acquisition device, an image sensor module comprises one or two or more than two image sensors; the control module is in electric signal connection with the image sensor module; the control module is in electric signal connection with the light source module; the image sensor module and the light source module are oppositely arranged above or below the sample accommodating module; a sample accommodating cavity in the sample accommodating module is used for bearing a sample; the light source module is used for emitting light rays, and the light rays penetrate through the sample accommodating module and project image information of the visible components onto the image sensor module; the sensor image includes a visible component image. The image acquisition module is used for acquiring images of the antigen-antibody agglomerated microparticles and projecting image information of the agglomerated microparticles onto the image sensor module; the image sensor module obtains a sensor image; the sensor image comprises an agglomerated microparticle image. The microscopic camera shoots the sample accommodating module to obtain a microscopic image; the microscopic image comprises an agglomerated microparticle image.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field related to formed element analysis, and particularly relates to an image acquisition device and method for formed element analysis. BACKGROUND

[0002] Detection of body fluids, excreta or secretions of organisms, such as blood, feces and urine, is a routine detection item. Through detection, the morphology and constituent information of various material components contained therein can be obtained, which is helpful for the diagnosis of disease types and disease courses. The most common feces detection is to distinguish and identify the components therein with the aid of a microscope, and to perform microscopic examination with the microscope.

[0003] The applicant has proposed a series of Chinese patents, such as

[0004] 1. CN2020112669290, "Cell analysis method and system and quantitative method and system";

[0005] 2. CN2020112669182, "Cell suspension sample imaging method and system and kit";

[0006] 3. CN2022104799126, "Microscopic image acquisition device rapid focusing method and microscopic image acquisition method";

[0007] 4. CN2023105340140, "Fecal component antigen antibody microparticle detection method and system, and turbidimetric dilution card and method";

[0008] 5. CN2023113529188, "Fecal suspension liquid formed component antigen antibody microparticle detection method and device";

[0009] 6. CN2023103592077, "Fecal formed component quantitative analysis and control method and system based on microscopic images";

[0010] With a brand-new technical solution, the content of target objects in various body fluids such as blood, urine and feces is measured, and a brand-new technical route is opened up to detect formed components in suspension liquid.

[0011] Regardless of the formed component image analysis of any body fluid, image acquisition is the key, and how to efficiently acquire high-quality pictures is the key. For some components that are difficult to observe with the naked eye, how to display them with the aid of technical means so that the image analysis tool can play a role is also a technical problem to be solved. SUMMARY

[0012] In the present application, the applicant proposes an image acquisition device and method, which combines an image sensor module, a sample containing module, a light source module, and a control module in one device to directly acquire images, thereby avoiding the need for complex optical structures and the process of adjusting focus or convergence in the prior art when acquiring images by using a microscope. By adding detection microparticles to the sample, the surface of the detection microparticles includes antigens or antibodies; the detection microparticles can bind to the to-be-detected substances in the sample to form microparticle binding bodies; the microparticle binding bodies aggregate to form agglomerated microparticle bodies; the size range of the agglomerated microparticle bodies is comparable to that of other formed components in the sample, and thus the image acquisition and analysis means of the formed components can be used to analyze and detect the corresponding components, thereby expanding the detectable components.

[0013] The technical solution of the present application solves the above technical problems. A detection image acquisition device is used for formed component detection and includes an image sensor module, a sample containing module, a light source module, and a control module. The image sensor module includes one or two or more image sensors. The control module is electrically connected to the image sensor module. The control module is electrically connected to the light source module. The image sensor module and the light source module are oppositely arranged above or below the sample containing module. The sample containing cavity in the sample containing module is used to carry a sample. The light source module is used to emit light, and the light passes through the sample containing module to project the image information of the formed components onto the image sensor module. The image sensor module obtains a sensor image. The sensor image includes the image of the formed components.

[0014] The pixel size of the image sensor of the image sensor module is less than 3 microns.

[0015] The detection image acquisition device further includes a lens module, which includes two or more lenses. The lenses correspond to the image sensors, and the lens module is located between the image sensor module and the sample containing module.

[0016] The image acquisition device is used for antigen-antibody detection and further includes detection microparticles. The surface of the detection microparticles includes antigens or antibodies. The detection microparticles are used to be added to a sample. The detection microparticles are used to bind to to-be-detected substances in the sample to form microparticle binding bodies. The microparticle binding bodies aggregate to form agglomerated microparticle bodies. The light source module is used to emit light, and the light passes through the sample containing module to project the image information of the agglomerated microparticle bodies onto the image sensor module. The image sensor module obtains a sensor image. The sensor image includes the image of the agglomerated microparticle bodies.

[0017] The formed components include any one or a combination of multiple of red blood cells, white blood cells, platelets, bacteria, worms, and worm eggs.

[0018] The technical solution of the application for solving the above technical problem can also be a detection image acquisition device for antigen-antibody agglomeration microparticle body image acquisition. The image acquisition device is used for antigen-antibody detection and includes a microscopic camera, a sample containing module, a light source module, and a control module. The control module is electrically connected to the microscopic camera and the light source module. The sample containing cavity in the sample containing module is used to carry the sample. The detection microparticles are also included. The surface of the detection microparticles includes antigens or antibodies. The detection microparticles are used to join the sample. The detection microparticles are used to combine with the detected substances in the sample to form microparticle combination bodies. The microparticle combination bodies are aggregated to form agglomeration microparticle bodies. The microscopic camera photographs the sample containing module to obtain a microscopic image. The microscopic image includes the agglomeration microparticle body image.

[0019] The detection image acquisition device includes a distance adjusting device. The distance adjusting device is used to adjust the distance between the microscopic camera and the sample containing module. The distance adjusting device is electrically connected to the control module. The magnification of the microscopic camera is equal to a set value.

[0020] The detection image acquisition device includes an image calculation module. The image calculation module is used to analyze the microscopic image or the sensor image, identify the agglomeration microparticle body image, and identify the agglomeration microparticle bodies less than the set value of the number of aggregated microparticles.

[0021] The set value of the number of aggregated microparticles is greater than 2 and less than 10.

[0022] The detection image acquisition device includes any one of the following technical features: TA1: The image calculation module is used to calculate the total area of the aggregated microparticles. The total area of the aggregated microparticles does not include the agglomeration microparticle bodies less than the set value of the number of aggregated microparticles. TA2: The image calculation module is used to calculate the total area of the aggregated microparticles. The total area of the aggregated microparticles includes the agglomeration microparticle bodies greater than the set value of the number of aggregated microparticles.

[0023] The technical solution of the application for solving the above technical problem can also be a detection image acquisition method. The method includes the following steps: detection microparticles are added to the sample. The surface of the detection microparticles includes antigens or antibodies. The sample is added to the sample containing cavity in the sample containing module. The detection microparticles combine with the detected substances in the sample to form microparticle combination bodies. The microparticle combination bodies are aggregated to form agglomeration microparticle bodies. Light passes through the sample containing module. The method also includes any one of the following technical features: TB1: The image information of the formed components is projected onto the image sensor module. The image sensor module obtains a sensor image. The sensor image includes the image of the formed components. TB2: The image information of the agglomeration microparticle bodies is projected onto the image sensor module. The image sensor module obtains a sensor image. The sensor image includes the agglomeration microparticle body image. TB3: A microscopic camera photographs the sample containing module to obtain a microscopic image. The microscopic image includes the agglomeration microparticle body image.

[0024] The technical effects of the above technical solutions include: the image sensor is sheet-shaped and closely arranged with the sample containing module to directly obtain images, the optical path is simple, and the structure is simple.

[0025] The technical effects of the above technical solutions include: the image sensor has a pixel size of less than 3 microns, which can ensure image acquisition accuracy and is suitable for detection and analysis of formed elements, including red blood cells, white blood cells, and various agglomerated microparticle images with a size of microns.

[0026] The technical effects of the above technical solutions include: the lens module can converge light to ensure that the exposure energy of each image sensor is sufficient.

[0027] The technical effects of the above technical solutions include: based on the agglomerated microparticle image, image analysis and detection of antigens and antibodies can be used to bring immunodetection into a new technical implementation system.

[0028] The technical effects of the above technical solutions include: simultaneous detection of formed elements and antigens and antibodies.

[0029] The technical effects of the above technical solutions include: the agglomerated microparticle image can also be obtained based on a microscope camera, which expands more acquisition methods.

[0030] The technical effects of the above technical solutions include: the distance adjusting device can ensure the clarity of the obtained images.

[0031] The technical effects of the above technical solutions include: agglomerated microparticles less than a set number of agglomerated microparticles are identified for subsequent identification and calculation, avoiding calculation errors introduced by occasional small numbers of microparticles.

[0032] The technical effects of the above technical solutions include: the detection image acquisition method is simple and efficient, and can simultaneously obtain formed element images and agglomerated microparticle images. BRIEF DESCRIPTION OF DRAWINGS

[0033] Figure 1 is a schematic block diagram of a detection image acquisition device Figure 1 ;

[0034] Figure 2 is a schematic block diagram of a detection image acquisition device Figure 2 ;

[0035] Figure 3 is a schematic diagram of a detection image acquisition device in an exploded state Figure 1 ;

[0036] Figure 4 is a schematic diagram of a detection image acquisition device in an exploded state Figure 2 ;

[0037] Figure 5 is a schematic diagram of a partially exploded state of the detection image acquisition device Figure 1 ;

[0038] Figure 6 is a schematic diagram of a partially exploded state of the detection image acquisition device

[0039] Figure 7 is a schematic diagram of a partially exploded state of the detection image acquisition device Figure 2 ;

[0040] Figure 8 is a top view of the sample containing module 1200 in the detection image acquisition device

[0041] Figure 9 is a schematic diagram of a partially exploded state of the detection image acquisition device Figure 3 ;

[0042] Figure 10 is a schematic diagram of a partially exploded state of the detection image acquisition device Figure 4 ;

[0043] Figure 11 is a schematic diagram of a partially exploded state of the detection image acquisition device Figure 3 ;

[0044] Figure 12 is a schematic diagram of a partially exploded state of the detection image acquisition device Figure 5 ;

[0045] Figure 13 is one of the images acquired by the detection image acquisition device

[0046] Figure 14 is a partial screenshot of the image acquired by the detection image acquisition device

[0047] Figures 15-a to 15-r is a screenshot of a plurality of formed components in the image acquired by the detection image acquisition device DETAILED DESCRIPTION

[0048] The present application will be further described in conjunction with the accompanying drawings. It should be noted that the following is a description of the preferred embodiments of the present application and does not constitute any limitation of the present application. The description of the preferred embodiments of the present application is only as an illustration of the general principles of the present application. The numbers "first", "second", and "A", "B" in the present application are only for the convenience of description and do not represent the time or spatial sequence relationship. The combination of letters and numbers "TA", "TB", "H" in the present application is only for the convenience of description and the specific meaning is determined by the specific words referred to.

[0049] As Figures 1 to 2An embodiment of the application is a detection image acquisition device for detecting a target substance, comprising an image sensor module, a sample containing module, a light source module, and a control module; the control module is electrically connected to the image sensor module; the control module is electrically connected to the light source module. The image sensor module and the light source module are oppositely arranged.

[0050] As Figure 1 An embodiment of the application is a detection image acquisition device, wherein the image sensor module and the light source module are oppositely arranged; the light source module is arranged above, and the image sensor module is arranged below; the sample containing module is arranged between the image sensor module and the light source module.

[0051] As Figure 2 An embodiment of the application is a detection image acquisition device, wherein other Figure 1 arrangements are the same, that is, the light source module is arranged below, and the image sensor module is arranged above.

[0052] As Figure 3 An embodiment of the application is a detection image acquisition device, wherein the sample containing cavity in the sample containing module 1200 is used for carrying a sample; the sheet-shaped image sensor 1300 is close to one side of the sample containing module 1200; the light source module 1100 emits light, and the light passes through the sample containing module 1200 to project the image information of the target substance onto the image sensor module 1300.

[0053] As Figures 3 to 4 An embodiment of the application is a detection image acquisition device, wherein the image sensor module comprises one or two or more than two image sensors 1300. Figure 3 The image sensor 1300 in the above

[0054] As Figures 3 to 5 An embodiment of the application is a detection image acquisition device, wherein the light source module 1100 is located above the image sensor 1300 and the sample containing module 1200.

[0055] As Figure 6 An embodiment of the application is a detection image acquisition device, wherein the light source module 1100 comprises a sheet-shaped light source body 1120 and a light homogenizer 1180; the sheet-shaped light source body 1120 is centrally distributed with a plurality of LED light sources 1122; the light emitted by the plurality of LED light sources 1122 is output after passing through the light homogenizer 1180. The output light is more uniform, and the consistency of the acquired image is improved. The light homogenizer comprises ground glass or multiple layers of ground glass.

[0056] As Figure 7An embodiment of the detection image acquisition device, the sample containing cavity 1230 in the sample containing module 1200 is used to carry various samples, and the samples contain formed components; the sample is in the form of a suspension liquid. The sample includes various body fluids such as blood, urine, fecal dilution samples, body cavity fluid, tissue fluid, and various samples to be detected. The formed components in the image include any one or more combinations of red blood cells, white blood cells, platelets, bacteria, worms, and worm eggs.

[0057] As Figure 7 and Figure 8 An embodiment of the detection image acquisition device, the sample containing module 1200 includes a sample containing cavity 1230 for carrying various samples, a sample inlet 1210, and an exhaust port 1220; light passes through the sample containing cavity 1230 for imaging projection.

[0058] Figure 7 In the image sensor 1330 of the image sensor module 1300, the image sensor 1 pixel size is less than 3 microns. It can be used to acquire micron-level image information. The image sensor module 1300 obtains a sensor image; the formed component image is included in the above-mentioned sensor image.

[0059] As Figure 13 The detection microparticles 7100 can be seen in the figure; the detection microparticle surface includes antigens or antibodies; the detection microparticles are used to add to the sample; the detection microparticles are used to combine with the to-be-detected substances in the sample to form microparticle combination bodies; the microparticle combination bodies are aggregated to form agglomerated microparticle bodies 7200. Figure 14 In the figure, the agglomerated microparticle body 7300 is also an agglomerated microparticle body. The light source module emits light to irradiate the sample containing module, and projects the image information of the agglomerated microparticle body 7200 or the agglomerated microparticle body 7300 onto the above-mentioned image sensor module; the image sensor module obtains a sensor image; the agglomerated microparticle body image is included in the above-mentioned sensor image.

[0060] When the sample is urine, the formed components in the image include any one or more of red blood cells as shown in Figure 15-a , white blood cells as shown in Figure 15-b , bacteria, worms, worm eggs, lipid droplets as shown in Figure 15-j , any one or more of coccus as shown in Figure 15-g , bacillus as shown in Figure 15-h , and yeast as shown in Figure 15-i . Also include some cells, such as epithelial cells as shown in Figure 15-e , renal tubular epithelial cells as shown in Figure 15-c , squamous epithelial cells as shown in Figure 15-d , and sperm as shown in Figure 15-f . Also include a variety of crystals, such as Figure 15-oAny one or more of the following: a magnesium ammonium phosphate crystal as shown in Figure 15-q Any one or more of the following: a calcium oxalate dihydrate crystal as shown in Figure 15-p Any one or more of the following: a calcium oxalate monohydrate crystal as shown in Figure 15-r Any one or more of the following: a calcium phosphate crystal as shown in Figure 15-k Any one or more of the following: a transparent tube type as shown in Figure 15-m Any one or more of the following: a granular tube type as shown in Figure 15-l Any one or more of the following: a cell tube type as shown in Figure 15-n Any one or more of the following: a waxy tube type as shown in

[0061] As shown in Figures 9 to 10 An embodiment of a detection image acquisition device includes an image sensor module 2300, a sample containing module 2200, a light source module 2100; further includes a lens module 2500, the lens module 2500 includes two or more lenses 2550, the above-mentioned lenses 2550 correspond to the above-mentioned image sensor 2330, the lens module 2500 is located between the image sensor module 2300 and the sample containing module 2200.

[0062] As shown in Figures 9 to 10 An embodiment of a detection image acquisition device, the sample containing module 2200 includes a sample containing cavity 2230 for carrying various samples, a sample inlet 2210, an exhaust port 2220; light passes through the sample containing cavity 2230 for imaging projection.

[0063] As shown in Figure 11 An embodiment of a detection image acquisition device, the above-mentioned image acquisition device is used for antigen-antibody detection; including a microscopic camera, a sample containing module, a light source module, a control module; the control module is electrically connected with the microscopic camera; the control module is electrically connected with the light source module; the sample containing cavity in the above-mentioned sample containing module is used for carrying samples; further includes detection microparticles; the surface of the detection microparticles includes antigens or antibodies; the detection microparticles are used for adding to the samples; the detection microparticles are used for combining with the to-be-detected substances in the samples to form microparticle combination bodies; the microparticle combination bodies gather to form agglomerated microparticle bodies; the microscopic camera photographs the sample containing module to obtain a microscopic image; the above-mentioned microscopic image includes an agglomerated microparticle body image.

[0064] As shown in Figure 12In one embodiment of an image acquisition detection device, the light source module 3100 includes a light source module body 3120 and a homogenizer 3180; the sample receiving module 3200 includes a sample receiving cavity 3230 for holding various samples, a sample inlet 3210, and an exhaust port 3220; the microscope camera includes an objective lens 3700, a lens barrel 3600, and a camera 3500. The light source module 3100 is planar and can be combined with the sample receiving module 3200 to form a whole, allowing relative movement with the microscope camera. It can be combined with the sample receiving module 3200 to move, or it can be fixed in position while the microscope camera moves.

[0065] In some embodiments of the image acquisition device not shown in the accompanying drawings, a phase-distance adjustment device is included; the phase-distance adjustment device is used to adjust the distance between the microscope camera and the sample containment module; the phase-distance adjustment device is electrically connected to the control module; the magnification of the microscope camera is equal to a set value. Adjusting the phase-distance adjustment device allows for the acquisition of clearly focused microscope images.

[0066] like Figures 1 to 2 ,as well as Figure 12 In one embodiment of an image acquisition device, an image calculation module is included. The image calculation module analyzes the aforementioned microscopic image or sensor image, identifies the aforementioned aggregated microparticle image, and identifies aggregated microparticles smaller than a set value for the number of aggregated microparticles. The set value for the number of aggregated microparticles is greater than 2 and less than 10.

[0067] Figure 13 In one embodiment of an image acquisition device, the image calculation module is used to calculate the total area of ​​aggregated microparticles, where the total area of ​​aggregated microparticles does not include agglomerated microparticles smaller than a set value for the number of aggregated microparticles; if the set value for the number of aggregated microparticles is 3, then... Figure 13 The aggregated microparticles (7200) are not included in the calculation of the total area of ​​aggregated microparticles. The total area of ​​aggregated microparticles includes aggregated microparticles larger than the set value for the number of aggregated microparticles. Figure 14 The aggregated microparticles 7200 in the calculation participate in the total area of ​​the aggregated microparticles.

[0068] In some embodiments of the detection image acquisition method not shown in the accompanying drawings, the steps include: adding detection particles to a sample, the surface of which includes antigens or antibodies; adding the sample to a sample receiving cavity in a sample receiving module; the detection particles and the analyte in the sample combining to form a particle conjugate; the particle conjugates aggregating to form aggregated microparticles; light passing through the sample receiving module; image information of the formed elements being projected onto the image sensor module; the image sensor module acquiring a sensor image; and the sensor image including the formed element image.

[0069] In some embodiments of the detection image acquisition method not shown in the accompanying drawings, the method comprises the following steps: adding detection microparticles into a sample, wherein the detection microparticles have a surface comprising antigens or antibodies; adding the sample into a sample holding cavity in a sample holding module; allowing the detection microparticles to bind with the analyte in the sample to form microparticle conjugates; allowing the microparticle conjugates to aggregate to form aggregated microparticle bodies; allowing light to pass through the sample holding module; projecting image information of the aggregated microparticle bodies onto the image sensor module; and obtaining a sensor image by the image sensor module, wherein the sensor image comprises an image of the aggregated microparticle bodies.

[0070] In some embodiments of the detection image acquisition method not shown in the accompanying drawings, the method comprises the following steps: adding detection microparticles into a sample, wherein the detection microparticles have a surface comprising antigens or antibodies; adding the sample into a sample holding cavity in a sample holding module; allowing the detection microparticles to bind with the analyte in the sample to form microparticle conjugates; allowing the microparticle conjugates to aggregate to form aggregated microparticle bodies; allowing light to pass through the sample holding module; taking a microscopic image of the sample holding module by a microscopic camera; and obtaining a microscopic image, wherein the microscopic image comprises an image of the aggregated microparticle bodies.

[0071] The present application is described and demonstrated according to preferred embodiments and several alternatives, but the application will not be limited by the specific description in the specification. Other additional alternatives or equivalent components can also be used to practice the present application.

Claims

1. A detection image acquisition device for detecting formed elements, characterized in that, comprising an image sensor module, a sample containing module, a light source module, a control module; the image sensor module comprises one or two or more image sensors; the control module is electrically connected with the image sensor module; the control module is electrically connected with the light source module; the image sensor module and the light source module are oppositely arranged above or below the sample containing module; the sample containing cavity in the sample containing module is used for carrying samples; the light source module is used for emitting light, and the light passes through the sample containing module to project the image information of formed elements onto the image sensor module; the image sensor module obtains a sensor image, and the sensor image comprises formed element images.

2. The detection image acquisition device according to claim 1, characterized in that, the pixel size of the image sensor of the image sensor module is less than 3 microns.

3. The detection image acquisition device according to claim 1, characterized in that, further comprising a lens module, the lens module comprises two or more lenses, the lenses correspond to the image sensors, and the lens module is located between the image sensor module and the sample containing module.

4. The detection image acquisition device according to claim 1, characterized in that, the image acquisition device is used for antigen-antibody detection; further comprising detection microparticles, the surface of the detection microparticles comprises antigens or antibodies, and the detection microparticles are used for adding to the sample; the detection microparticles are used for combining with the to-be-detected substances in the sample to form microparticle combination bodies; the microparticle combination bodies are aggregated to form agglomerated microparticle bodies; the light source module is used for emitting light, and the light passes through the sample containing module to project the image information of the agglomerated microparticle bodies onto the image sensor module; the image sensor module obtains a sensor image, and the sensor image comprises agglomerated microparticle body images.

5. The detection image acquisition device according to claim 1, characterized in that, the formed elements comprise any one or a combination of more than one of red blood cells, white blood cells, platelets, bacteria, worms, and worm eggs.

6. A detection image acquisition device for antigen-antibody agglomerated microparticle body image acquisition, characterized in that, the image acquisition device is used for antigen-antibody detection; comprising a microscopic camera, a sample containing module, a light source module, and a control module; the control module is electrically connected with the microscopic camera; the control module is electrically connected with the light source module; the sample containing cavity in the sample containing module is used for carrying samples; further comprising detection microparticles, the surface of the detection microparticles comprises antigens or antibodies, and the detection microparticles are used for adding to the sample; the detection microparticles are used for combining with the to-be-detected substances in the sample to form microparticle combination bodies; the microparticle combination bodies are aggregated to form agglomerated microparticle bodies; the microscopic camera photographs the sample containing module to obtain a microscopic image, and the microscopic image comprises agglomerated microparticle body images.

7. The detection image acquisition device according to claim 6, characterized in that, comprising a distance adjusting device, the distance adjusting device is used for adjusting the distance between the microscopic camera and the sample containing module; the distance adjusting device is electrically connected with the control module; and the magnification of the microscopic camera is equal to a set value. ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ 8. The detection image acquisition device according to claim 6, characterized in that, the image computing module is configured to analyze the microscopic image or the sensor image, identify the agglomerated particle body image, and identify the agglomerated particle body with a number less than the set value of the agglomerated particle.

9. The detection image acquisition device according to claim 8, characterized in that, the set value of the agglomerated particle is greater than 2 and less than 10.

10. The detection image acquisition device according to claim 8, characterized in that, any one of the following technical features is included: TA1: the image computing module is configured to calculate the total area of the agglomerated particles, and the total area of the agglomerated particles does not include the agglomerated particle body with a number less than the set value of the agglomerated particle; TA2: the image computing module is configured to calculate the total area of the agglomerated particles, and the total area of the agglomerated particles includes the agglomerated particle body with a number greater than the set value of the agglomerated particle.

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