Detection sample containing and detecting device

Abstract

The detection sample accommodating and detecting device comprises more than two microscopic examination parts; the microscopic examination part comprises a detection part A1; the detection part A1 comprises a cavity A1 and a sample adding port A1, and the cavity A1 is communicated with the sample adding port A1; the cavity A1 is used for containing a sample A1, the cavity A1 is used for shooting a microscopic magnification image of the microscopic examination sample A1, the sample A1 is obtained by adding detection particles into the sample, and the detection particles and a target object are combined and gathered to form agglomerated particles; shooting the sample A1 in the cavity A to obtain a microscopic image of the agglomerated microparticles or the detected microparticles; performing image analysis on the microscopic image to obtain the content of the agglomerated microparticles, and obtaining the content of the antigen A1 or the antibody A1 in the sample A1 according to the content. The detection part B comprises a cavity B and a sample adding port B, the cavity B is used for containing a sample B, the sample adding port B is communicated with the cavity B, the cavity B is used for optical detection analysis of the sample, and the optical detection analysis is used for detection-free epidemic analysis of the sample B.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of medical equipment, and particularly relates to a formed component detection container and a detection device and a detection method. BACKGROUND

[0002] Medical detection analysis devices include various instruments, which can be classified in various ways according to functions or detection principles, such as:

[0003] Type A: detection devices based on optical reaction characteristics of samples, such as flow cytometers, biochemical analysis instruments, etc. The detection of optical energy based on the transmission, scattering or absorption characteristics of different substances in the sample is used to obtain component or content information of the corresponding components.

[0004] Type B: detection devices for quantitative and qualitative detection of trace amounts of immune markers. The principles of such instruments are complex, including enzyme-linked immunosorbent assay, radioimmunoassay, immune turbidimetry, immunofluorescence analysis, and chemiluminescence immunoassay. Most of them use optical characteristics for detection.

[0005] Patients need to use different instruments for various detections when they enter a hospital, which is time-consuming and costly.

[0006] With the development of the pet economy, pet hospitals require more and more high-end instruments, but pet hospitals are sensitive to the cost of instrument investment, and the area of pet hospitals is limited, making it difficult to place more instruments and equipment.

[0007] Therefore, how to reduce the types and quantities of sample collection in the detection process, how to complete multiple detections in one detection process, especially how to complete cross-sample type and cross-detection principle detection in one detection, are technical problems to be solved by the present application. SUMMARY

[0008] The present application proposes to integrate one or more immune detection sites in a detection sample container, and to pressurize the detection sites, so that one detection container can support formed component detection and / or immune detection, and proposes a detection device supporting multiple technical types.

[0009] The solution of the technical problem of the present application is a detection sample containing device, which comprises two or more microscopic detection parts; the microscopic detection part comprises a detection part A; the detection part A comprises a cavity A and a sample adding port A, the cavity A is communicated with the sample adding port A, the cavity A is used for containing a sample A, the cavity A is used for shooting a microscopic magnified image of the microscopic sample, and the microscopic magnified image is used for forming component analysis; the detection device further comprises a detection part B, the detection part B comprises a cavity B and a sample adding port B, the cavity B is used for containing a sample B, the sample adding port B is communicated with the cavity B, the cavity B is used for optical detection analysis of the sample, and the optical detection analysis is used for sample B immune detection analysis.

[0010] The solution of the technical problem of the present application is a detection sample containing device, which comprises two or more microscopic detection parts; the microscopic detection part comprises a detection part A; the detection part A comprises a cavity A and a sample adding port A, the cavity A is communicated with the sample adding port A, the cavity A is used for containing a sample A, the cavity A is used for shooting a microscopic magnified image of the microscopic sample, and the microscopic magnified image is used for forming component analysis; the detection device further comprises a detection part B, the detection part B comprises a cavity B and a sample adding port B, the cavity B is used for containing a sample B, the sample adding port B is communicated with the cavity B, the cavity B is used for optical detection analysis of the sample, and the optical detection analysis is used for sample B immune detection analysis.

[0011] The sample can be blood, and the stratification information is used for detecting the hematocrit of the blood.

[0012] The detection device can further comprise at least one reagent containing part C, the reagent containing part C comprises a cavity C and a sample adding port C, the cavity C is used for containing a detection reagent, and the sample adding port C is upwardly opened, liquid is added to the cavity C through the sample adding port C or liquid is taken out from the cavity C.

[0013] The cavity C is respectively used for containing a staining reagent or an immune detection reagent.

[0014] The detection device can further comprise at least one sample preparation part D, the sample preparation part D comprises a cavity D and a sample adding port D; one cavity D is used for sample preparation, the sample adding port D is upwardly opened, liquid is added to the cavity D through the sample adding port D or liquid is taken out from the cavity D; and a lower section of the cavity D is a conical cavity.

[0015] The solution of the technical problem of the application can also be a detection device for cooperating with a detection sample containing device, the microscopic detection part includes detection part A1 and detection part A2; the detection part A1 includes cavity A1, and the detection part A2 includes cavity A2; the cavity A1 is used for containing sample A1, and the cavity A2 is used for containing sample A2; the detection device includes a digital microscope assembly and a bearing assembly; the bearing assembly includes an X-direction sliding table assembly; the detection sample containing device is placed on the X-direction sliding table assembly, and the X-direction sliding table assembly drives the detection sample containing device to move in the X-direction; the X-direction sliding table assembly moves to position A1, and the digital microscope assembly is used for shooting an image of the microscopic sample through the outer wall of the cavity A1; an image of the sample A1 is obtained; the X-direction sliding table assembly moves to position A2, and the digital microscope assembly is used for shooting an image of the microscopic sample through the outer wall of the cavity A2; an image of the sample A2 is obtained.

[0016] The microscopic detection part can include detection part A3 and detection part A4; the detection part A3 includes cavity A3, and the detection part A4 includes cavity A4; the cavity A3 is used for containing sample A3, and the cavity A4 is used for containing sample A4; the bearing assembly further includes a Y-direction sliding table assembly; the Y-direction sliding table assembly drives the X-direction sliding table assembly to move in the Y-direction.

[0017] The detection device can further include a layered detection assembly; the layered detection assembly is used for calculating the volume of the components according to the static layered information of the sample E in the cavity E.

[0018] The layered detection assembly can include a camera, which obtains a photo of the sample E, and obtains the layered information of the sample E through the photo.

[0019] The optical detection assembly can include a light source and a light intensity detection assembly; the light source irradiates the cavity B, and the light intensity detection assembly obtains light passing through the cavity B to obtain the optical reaction information of the sample B.

[0020] The solution of the technical problem of the application can also be a detection method based on a detection sample containing device, which includes two or more microscopic detection parts; the microscopic detection part includes detection part A1; the detection part A1 includes cavity A1 and sample adding port A1, and the cavity A1 is communicated with the sample adding port A1; the cavity A1 is used for containing sample A1, the cavity A1 is used for shooting a microscopic magnified image of the microscopic sample A1, the sample A1 is obtained by adding a detection particle to a sample, the detection particle is combined with a target object to form a group of agglomerated particle bodies; the sample A1 in the cavity A1 is shot to obtain a microscopic image of the group of agglomerated particle bodies or the detection particle; the microscopic image is subjected to image analysis to obtain the content of the group of agglomerated particle bodies, and the content of the antigen A1 or the antibody A1 in the sample A1 is obtained according to the content.

[0021] The microscope detection part can include a detection part A2; the detection part A2 includes a cavity A2 and a sample adding port A2, and the cavity A2 is communicated with the sample adding port A2; the cavity A2 is used for accommodating a sample A2, and the cavity A2 is used for shooting a microscopic magnified image of the detection sample A2; the sample A2 is obtained by adding a detection particle to a sample; the detection particle is combined and aggregated with a target object to form a group particle body; the sample A2 in the cavity A2 is shot to obtain a microscopic image of the group particle body or the detection particle; image analysis is performed on the microscopic image to obtain the content of the group particle body, and the content of the antigen A2 or the antibody A2 in the sample A2 is obtained according to the content.

[0022] The microscope detection part can include a detection part A3; the detection part A3 includes a cavity A3 and a sample adding port A3, and the cavity A3 is communicated with the sample adding port A3; the cavity A3 is used for shooting a microscopic magnified image of the detection sample A3, and the microscopic magnified image is used for component analysis.

[0023] The detection sample containing device can further include at least one reagent containing part C, and the reagent containing part C includes a cavity C and a sample adding port C; one cavity C is used for containing an immunological detection reagent, and the sample adding port C is upwardly opened; liquid is added to the cavity C through the sample adding port C or liquid is taken out from the cavity C; and the immunological detection reagent includes detection particles.

[0024] In the plurality of reagent containing parts C of the detection sample containing device, one cavity C is used for containing a staining reagent.

[0025] The detection sample containing device can further include a detection part B, which is used for optical detection analysis of a sample; the detection part B includes a cavity B and a sample adding port B; the cavity B is used for containing a sample B; the sample adding port B is communicated with the sample adding port A; the cavity B is used for optical detection analysis of the sample; the optical detection analysis is used for immunological analysis of the sample B; and the sample adding port B is consistent with the sample adding port A in height.

[0026] The target object can include any one of a protein, a saccharide, a lipid, a vitamin and a small molecule hormone; the detection particle surface is marked with an antibody, and is combined and aggregated as an affinity reaction.

[0027] The target object can include an antibody; the detection particle surface includes any one of a protein, a saccharide, a lipid, a vitamin and a small molecule hormone, and is combined and aggregated as an affinity reaction.

[0028] The detection particle surface is marked with a specific antigen or antibody, and is combined and aggregated as specific antigen-antibody combination.

[0029] The detection particle surface is marked with a specific antibody, and is combined and aggregated as an immunological reaction.

[0030] The group particle body is formed by combination and aggregation of a cell surface antigen in a sample and an antibody on a particle surface.

[0031] The agglomeration microparticle body can be the binding of the cell surface antibody in the sample to the antigen on the surface of the microparticle.

[0032] The antibody / antigen on the surface of the detection microparticle can be modified with a quenching group. When the detection microparticle is attracted to the corresponding antigen / antigen, the fluorescent group is quenched and no longer emits fluorescence. The more the aggregation, the less the fluorescence.

[0033] The antibody / antigen on the surface of the detection microparticle can be modified with a fluorescent group. When the detection microparticle is attracted to the corresponding antigen / antigen, the fluorescent group is excited to emit fluorescence. The more the aggregation, the more the fluorescence.

[0034] The detection part E can be further included for sample stratification detection. The detection part E includes a cavity E and a sample inlet E, and the cavity E is in communication with the sample inlet E. The cavity E is used to accommodate the sample E to be stratified. The stratification information of the sample E is used to calculate the volume of the formed component.

[0035] The sample can be blood, and the stratification information can be used to detect the hematocrit of the blood.

[0036] The technical effect of the above technical solution is that the cavity A suitable for microscopic digital imaging can present the sample in a flattened state, and the cavity B suitable for light irradiation and detection, so that the sample container can be applied to multiple functional detection scenarios.

[0037] The technical effect of the above technical solution is that the cavity E can be used to accommodate the sample E to be stratified, so that the volume or volume ratio of the corresponding component can be calculated based on the stratification. The sample container can be applied to more functional detection scenarios.

[0038] The technical effect of the above technical solution is that the reagent container C provides a reagent storage space and integrates reagents to make the preparation and detection process more efficient.

[0039] The technical effect of the above technical solution is that the sample preparation part D provides a space for reagents and samples to be prepared nearby.

[0040] The technical effect of the above technical solution is that the cavity A has multiple cavities, which can be more freely combined for detection items. One part of the cavity A can be used for formed component detection, and another part of the cavity A can be used for immune detection based on detection microparticles. Each cavity A can be used for formed component detection of different items. Each cavity A can be used for immune detection of different items. It is a more efficient and comprehensive solution. The immune detection based on detection microparticles is different from the traditional immune detection method, which is based on the image feature change caused by the agglomeration of detection microparticles in the microscopic magnified image.

[0041] The technical effects of the above technical solutions are: the cavity C in the reagent containing part C is used for containing a staining reagent; or the cavity C is used for containing an immunological detection reagent or a biochemical detection reagent; a storage space is provided for the reagent in the process, so that the reagent and the sample containing device can be integrated.

[0042] The technical effects of the above technical solutions are: the cavity A is used for microscopic digital imaging analysis of the formed components, and the cavity B is used for optical detection and analysis of the sample, so that the detection sample containing device is suitable for different application scenarios and can also be suitable for comprehensive application scenarios, and can be used for microscopic digital imaging analysis of the formed components, biochemical detection and immunological detection analysis. The material of the cavity B meets the corresponding optical characteristic requirements and can be glass, sapphire, transparent plastic or other materials with different optical properties.

[0043] The technical effects of the above technical solutions are: based on the agglomeration of detection particles, the optical characteristic detection is performed according to the change of the optical properties of the sample B, and the immunological detection or biochemical detection is realized.

[0044] The technical effects of the above technical solutions are: the cavity D in the sample preparation part D provides a space for the preparation of the sample, and the same sample containing device can support the whole process of reagent packaging, sample preparation and sample detection.

[0045] The technical effects of the above technical solutions are: the detection part A and the detection part B are located on the same side or different sides of the detection sample containing device, which facilitates the setting of different detection components and has better adaptability and flexibility.

[0046] The technical effects of the above technical solutions are: microscopic magnified images can be obtained; based on the images, formed component analysis of multiple detection parts is performed, multiple detection parts can detect different samples, and efficiency is improved. At the same time, immunological analysis of the sample can also be performed according to the optical reaction characteristics of the sample.

[0047] The technical effects of the above technical solutions are: the cavity A is used for formed component analysis, and the cavity E is used for containing a sample E static layer, which provides a basis for more accurate volume ratio calculation.

[0048] The technical effects of the above technical solutions are: the cavity E and the cavity A each have their own sample adding port, which facilitates the addition of samples in different states or different samples. In some cases, samples with different dilution ratios can be added to facilitate corresponding detection. The cavity E and the cavity A can not be connected; in some cases, the cavity E and the cavity A can be connected.

[0049] The technical effects of the above technical solutions are: the cavity A is used for formed component analysis, including morphological analysis, biochemical analysis and immunological detection analysis, and can perform analysis of multiple items, which has good adaptability.

[0050] The technical effects of the above technical solutions are that the cross section of the sample adding port E is larger than the cross section of the cavity E, the sample adding is facilitated, the liquid in the cavity E is facilitated to be collected and layered, and the calculation accuracy is improved.

[0051] The technical effects of the above technical solutions are that the sample is blood, the layering information is used for detecting the hematocrit of the blood, and the multi-cavity detection sample containing device can be used for microscopic magnified image analysis of formed elements and also can accurately calculate the hematocrit.

[0052] The technical effects of the above technical solutions are that the reagent containing part C can increase the containing space of the multi-path detection reagent as the detection part B.

[0053] The technical effects of the above technical solutions are that the cavity C in the reagent containing part C is used for containing a staining reagent, or the cavity C is used for containing an immunological detection reagent or a biochemical detection reagent, and storage space is provided for the reagent in the process, so that the reagent and the sample containing device can be integrated.

[0054] The technical effects of the above technical solutions are that the cavity heights of the cavity B, the cavity C and the cavity E are consistent, and the manufacturing is facilitated and the sample storage volumes between different cavities are compared.

[0055] The technical effects of the above technical solutions are that the detection device can obtain a microscopic magnified image, can perform formed element analysis and immunological analysis based on the image, and can also perform accurate hematocrit calculation.

[0056] The technical effects of the above technical solutions are that the detection device can perform formed element analysis based on a microscopic image, can perform hematocrit calculation, and can also perform immunological analysis of the sample according to the optical reaction characteristics of the sample. BRIEF DESCRIPTION OF DRAWINGS

[0057] Figure 1 is a schematic diagram of a detection sample containing device;

[0058] Figure 2 is a schematic diagram of a detection sample containing device in an exploded state;

[0059] Figure 3 is a schematic diagram of a detection sample containing device;

[0060] Figure 4 is a schematic diagram of a detection sample containing device;

[0061] Figure 5 is a schematic diagram of a detection sample containing device;

[0062] Figure 6 is a schematic diagram of a detection sample containing device in a partially exploded state;

[0063] Figure 7is a partial view of a detection sample container device;

[0064] Figure 8 is a partial view of a detection sample container device;

[0065] Figure 9 is a schematic view of a detection sample container device;

[0066] Figure 10 is a partial sectional view of a detection sample container device;

[0067] Figure 11 is a schematic block diagram of a detection device;

[0068] Figure 12 is a schematic view of a detection device. DETAILED DESCRIPTION

[0069] The content of the present application will be further described in conjunction with the accompanying drawings.

[0070] It should be noted that the following is a description of the preferred embodiments of the present application and does not constitute any limitation on the present application. The description of the preferred embodiments of the present application is only as an illustration of the general principles of the present application. The embodiments described in the present application are only a part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0071] In the description of the present application, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the purpose of facilitating the description of the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first", "second", and technical features numbered with Arabic numerals 1, 2, 3, and the like, as well as "A" and "B" numbers, are only for the purpose of description, and are only for the convenience of explanation, and do not represent the temporal or spatial sequence relationship; cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features limited by "first", "second", and technical features numbered with Arabic numerals 1, 2, 3, and the like can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "several" is two or more, unless otherwise explicitly specified.

[0072] As Figure 1The application discloses a detection sample containing device, which comprises two or more microscopic detection parts; the microscopic detection part comprises a detection part A; the detection part A comprises a cavity A, i.e., a formed element detection cavity, and a sample adding port A, the cavity A is communicated with the sample adding port A, the cavity A is used for containing a sample A, the cavity A is used for shooting a microscopic magnified image of the microscopic sample, and the microscopic magnified image is used for formed element analysis; the detection device further comprises a detection part B, the detection part B comprises a cavity B, i.e., an optical detection cavity, and a sample adding port B, the cavity B is used for containing a sample B, the sample adding port B is communicated with the cavity B, the cavity B is used for optical detection analysis of the sample, and the optical detection analysis is used for sample B immune detection analysis.

[0073] As Figures 1 to 9 , the microscopic detection part comprises a detection part A1, a detection part A2, a detection part A3 and a detection part A4; the detection part A1 comprises a cavity A1, a sample adding port A1 and an exhaust port A1; the detection part A2 comprises a cavity A2, a sample adding port A2 and an exhaust port A2; the detection part A3 comprises a cavity A3, a sample adding port A3 and an exhaust port A3; and the detection part A4 comprises a cavity A4, a sample adding port A4 and an exhaust port A4. Figure 8 The sample adding port A1 and the sample adding port A2 are symmetrically arranged; and the sample adding port A3 and the sample adding port A4 are symmetrically and adjacently arranged, so that the sample adding is facilitated. Figure 7 And Figure 8 The detection part A1, the detection part A2, the detection part A3 and the detection part A4 can be added with different samples or the same sample.

[0074] As Figure 5 , the cavity A of the detection part A is a shallow cavity, which is suitable for flat expansion of formed elements in the sample. Figure 6 The detection part A is further provided with a cavity cover. Figure 7 The detection part A can not be designed with the cavity cover.

[0075] As Figure 9 , the application discloses a detection sample containing device, which comprises two or more microscopic detection parts; the microscopic detection part comprises a detection part A; the detection part A comprises a cavity A, i.e., a formed element detection cavity, and a sample adding port A, the cavity A is communicated with the sample adding port A, the cavity A is used for containing a sample A, the cavity A is used for shooting a microscopic magnified image of the microscopic sample, and the microscopic magnified image is used for formed element analysis; the detection device further comprises a detection part E, which is used for sample layering detection, and the detection part E comprises a cavity E, i.e., a pressure accumulation detection cavity, and a sample adding port E, the cavity E is communicated with the sample adding port E; the cavity E is used for containing a sample E for static layering; and layering information of the sample E is used for calculating the volume of components in the formed elements. The sample is blood, and the layering information is used for detecting the pressure accumulation of the blood. Figure 10 The sample adding port E is larger than the cavity E in cross section; the sample adding port E is convenient for adding the sample, the cavity E is convenient for collecting and layering liquid in the cavity E, and the calculation accuracy is improved.

[0076] As Figure 3, the embodiment of the detection sample containing device further comprises at least one reagent containing part C, the reagent containing part C comprises a cavity C for containing detection reagents, and a sample adding port C opening upward for adding liquid to the cavity C or taking out liquid from the cavity C. The cavity C is used for containing a staining reagent or containing an immunological detection reagent. Figure 4 The cavity C can be multiple.

[0077] As shown in Figures 1 to 3 , the embodiment of the detection sample containing device further comprises at least one sample preparation part D, the sample preparation part D comprises a cavity D and a sample adding port D; one cavity D is used for sample preparation, and the sample adding port D opens upward for adding liquid to the cavity D or taking out liquid from the cavity D; as shown in Figure 4 , the lower section of the cavity D is a conical cavity. As shown in Figure 4 The cavity D can be multiple.

[0078] A detection device for cooperating with the detection sample containing device as shown in Figures 1 to 9 , the microscopic examination part comprises detection part A1 and detection part A2; the detection part A1 comprises a cavity A1, and the detection part A2 comprises a cavity A2; the cavity A1 is used for containing a sample A1, and the cavity A2 is used for containing a sample A2.

[0079] As shown in Figure 11 and Figure 12 , in an embodiment of the detection device, a digital microscope assembly and a bearing assembly are included; the bearing assembly comprises an X-direction sliding table assembly; the detection sample containing device is placed on the X-direction sliding table assembly, and the X-direction sliding table assembly drives the detection sample containing device to move in the X-direction; the X-direction sliding table assembly moves to position A1, and the digital microscope assembly is used for photographing an image of a microscopic examination sample in the cavity A1; an image of the sample A1 is obtained; the X-direction sliding table assembly moves to position A2, and the digital microscope assembly is used for photographing an image of a microscopic examination sample in the cavity A2; an image of the sample A2 is obtained.

[0080] As shown in Figures 1 to 9 , the microscopic examination part comprises detection part A3 and detection part A4; the detection part A3 comprises a cavity A3, and the detection part A4 comprises a cavity A4; the cavity A3 is used for containing a sample A3, and the cavity A4 is used for containing a sample A4; the bearing assembly further comprises a Y-direction sliding table assembly; the Y-direction sliding table assembly drives the X-direction sliding table assembly to move in the Y-direction.

[0081] As shown in Figure 11 and Figure 12 , in an embodiment of the detection device, a layered detection assembly is further included; the layered detection assembly is used for calculating the volume of components according to the static layered information of a sample E in a cavity E. The layered detection assembly comprises a camera device, the camera device obtains a photo of the sample E, and the layered information of the sample E is obtained through the photo.

[0082] As Figure 11 And Figure 12 In an embodiment of the detection device, the optical detection assembly comprises a light source and a light intensity detection assembly; the light source irradiates the cavity B, and the light intensity detection assembly obtains the light passing through the cavity B to obtain the optical reaction information of the sample B.

[0083] As Figures 1 to 9 In an embodiment of the detection method, the detection sample containing device comprises two or more microscopic detection parts; the microscopic detection part comprises a detection part A1; the detection part A1 comprises a cavity A1 and a sample adding port A1, and the cavity A1 is in communication with the sample adding port A1; the cavity A1 is used for containing a sample A1, and the cavity A1 is used for shooting a microscopic magnified image of the microscopic sample A1; the sample A1 is obtained by adding detection microparticles to a sample, the detection microparticles are combined with target objects to form agglomerated microparticle bodies; the sample A1 in the cavity A is shot to obtain a microscopic image of the agglomerated microparticle bodies or the detection microparticles; the microscopic image is subjected to image analysis to obtain the content of the agglomerated microparticle bodies, and the content of the antigen A1 or the antibody A1 in the sample A1 is obtained according to the content.

[0084] As Figures 1 to 9 The microscopic detection part comprises a detection part A2; the detection part A2 comprises a cavity A2 and a sample adding port A2, and the cavity A2 is in communication with the sample adding port A2; the cavity A2 is used for containing a sample A2, and the cavity A2 is used for shooting a microscopic magnified image of the microscopic sample A2; the sample A2 is obtained by adding detection microparticles to a sample, the detection microparticles are combined with target objects to form agglomerated microparticle bodies; the sample A2 in the cavity A is shot to obtain a microscopic image of the agglomerated microparticle bodies or the detection microparticles; the microscopic image is subjected to image analysis to obtain the content of the agglomerated microparticle bodies, and the content of the antigen A2 or the antibody A2 in the sample A2 is obtained according to the content.

[0085] As Figures 1 to 9 The microscopic detection part comprises a detection part A3; the detection part A3 comprises a cavity A3 and a sample adding port A3, and the cavity A3 is in communication with the sample adding port A3; the cavity A3 is used for shooting a microscopic magnified image of a microscopic sample A3, and the microscopic magnified image is used for component analysis.

[0086] As Figures 1 to 9 The embodiment of the detection sample containing device further comprises at least one reagent containing part C, and the reagent containing part C comprises a cavity C and a sample adding port C; one cavity C is used for containing an immunological detection reagent, and the sample adding port C is upwardly opened; liquid is added to the cavity C through the sample adding port C or liquid is taken out; the immunological detection reagent comprises detection microparticles. In the plurality of reagent containing parts C of the detection sample containing device, one cavity C is used for containing a staining reagent.

[0087] As Figures 1 to 9The embodiment of the detection sample containing device also comprises a detection part B for optical detection analysis of the sample, the detection part B comprises a cavity B for containing the sample B and a sample adding port B, the cavity B is communicated with the sample adding port B, the cavity B is used for optical detection analysis of the sample, and the optical detection analysis is used for sample B immune detection analysis; the sample adding port B is consistent with the sample adding port A in height. The overall detection sample containing device is convenient to translate on the bearing assembly, and the sample adding operation control is also convenient.

[0088] In an embodiment of the detection method, the target object comprises any one of a protein, a saccharide, a lipid, a vitamin, and a small molecule hormone; the detection micro-particle surface is marked with an antibody, and is combined and aggregated into an affinity reaction.

[0089] In an embodiment of the detection method, the target object comprises an antibody; the detection micro-particle surface comprises any one of a protein, a saccharide, a lipid, a vitamin, and a small molecule hormone, and is combined and aggregated into an affinity reaction.

[0090] In an embodiment of the detection method, the detection micro-particle surface is marked with a specific antigen or antibody, and is combined and aggregated into specific antigen-antibody combination.

[0091] In an embodiment of the detection method, the detection micro-particle surface is marked with a specific antibody, and is combined and aggregated into an immune reaction.

[0092] In an embodiment of the detection method, the aggregated micro-particle body is combined by a cell surface antigen in a sample and an antibody on a micro-particle surface.

[0093] In an embodiment of the detection method, the aggregated micro-particle body is combined by a cell surface antibody in a sample and an antigen on a micro-particle surface.

[0094] In an embodiment of the detection method, the detection micro-particle surface is marked with an antibody / antigen modified with a quencher group, when the detection micro-particle is attracted and aggregated by a corresponding antigen / antibody, the fluorescent group is quenched, and no longer emits fluorescence, and the more the aggregation, the less the fluorescence.

[0095] In an embodiment of the detection method, the detection micro-particle surface is marked with an antibody / antigen modified with a fluorescent group, when the detection micro-particle is attracted and aggregated by a corresponding antigen / antibody, the fluorescent group is excited to emit fluorescence, and the more the aggregation, the more the fluorescence.

[0096] As Figure 9 The embodiment also comprises a detection part E for layered detection of the sample, the detection part E comprises a cavity E and a sample adding port E, and the cavity E is communicated with the sample adding port E; the cavity E is used for containing the sample E to be statically layered; and layered information of the sample E is used for calculating the volume of components in the formed components. The layered information is used for detecting the hematocrit of the blood. In some embodiments, a scale line is arranged on the cavity for detecting the hematocrit, and is used for distinguishing the layered state information.

[0097] While the application has been illustrated and described in accordance with preferred embodiments and several alternatives, it is not intended to be limited to the details described. Other alternative or equivalent components can be used in practicing the application.

Claims

1. A detection sample container, comprising two or more microscopic detection parts, wherein the microscopic detection parts comprise a detection part A, the detection part A comprises a cavity A and a sample inlet A, the cavity A is in communication with the sample inlet A, the cavity A is used for containing a sample A, the cavity A is used for taking a microscopic magnified image of the sample for microscopic component analysis.

2. A detection sample container, comprising two or more microscopic detection parts, wherein the microscopic detection parts comprise a detection part A, the detection part A comprises a cavity A and a sample inlet A, the cavity A is in communication with the sample inlet A, the cavity A is used for containing a sample A, the cavity A is used for taking a microscopic magnified image of the sample for microscopic component analysis.

3. The detection sample container according to claim 1 or 2, further comprising a detection part B, the detection part B comprises a cavity B and a sample inlet B, the cavity B is used for containing a sample B, the cavity B is in communication with the sample inlet B, the cavity B is used for optical detection analysis of the sample, and the optical detection analysis is used for immune detection analysis of the sample B.

4. The detection sample container according to claim 1 or 2, further comprising at least one reagent container C, the reagent container C comprises a cavity C and a sample inlet C, the cavity C is used for containing a detection reagent, and the sample inlet C is upwardly open, and the cavity C is used for adding or removing liquid through the sample inlet C.

5. The detection sample container according to claim 4, wherein the cavity C is used for containing a staining reagent or an immune detection reagent.

6. The detection sample container according to claim 1 or 2, further comprising at least one sample preparation part D, the sample preparation part D comprises a cavity D and a sample inlet D, one cavity D is used for sample preparation, the sample inlet D is upwardly open, and the cavity D is used for adding or removing liquid through the sample inlet D, and the lower part of the cavity D is a conical cavity.

7. The detection sample container according to claim 1 or 2, wherein the microscopic detection parts comprise a detection part A1 and a detection part A2, the detection part A1 comprises a cavity A1, and the detection part A2 comprises a cavity A2, the cavity A1 is used for containing a sample A1, and the cavity A2 is used for containing a sample A2.

8. The detection sample container according to claim 1 or 2, further comprising a digital microscope assembly and a bearing assembly, the bearing assembly comprises an X-direction slide assembly.

9. The detection sample container according to claim 1 or 2, wherein the detection sample container is placed on the X-direction slide assembly, and the X-direction slide assembly drives the detection sample container to move in the X-direction.

10. The detection sample container according to claim 1 or 2, wherein the X-direction slide assembly moves to a position A1, and the digital microscope assembly is used for taking an image of the sample through the outer wall of the cavity A1 to obtain an image of the sample A1.

11. The detection sample container according to claim 1 or 2, wherein the X-direction slide assembly moves to a position A2, and the digital microscope assembly is used for taking an image of the sample through the outer wall of the cavity A2 to obtain an image of the sample A2.

3. The sample containment device of claim 2, wherein, ​ ​ ​ ​ ​ ​ ​ ​ ​ 7. A detection device for use with a sample containment device according to any one of claims 1 to 6, characterised in that, ​ ​ ​ ​ ​ 8. The detection device of claim 7, wherein, The microscopic detection part includes detection part A3 and detection part A4; the detection part A3 includes cavity A3, and the detection part A4 includes cavity A4; the cavity A3 is used for accommodating sample A3, and the cavity A4 is used for accommodating sample A4; The bearing assembly further includes a Y-direction sliding table assembly; the Y-direction sliding table assembly drives the X-direction sliding table assembly to move in the Y direction.

9. The detection device of claim 7, wherein, Further comprising a layered detection assembly; the layered detection assembly is used for calculating the volume of components according to the static layered information of sample E in the cavity E.

10. The detection device of claim 9, wherein, The layered detection assembly includes a camera, which obtains a photo of sample E, and obtains the layered information of sample E through the photo.

11. The detection device of claim 7, wherein, The optical detection assembly includes a light source and a light intensity detection assembly; the light source irradiates the cavity B, and the light intensity detection assembly obtains light passing through the cavity B to obtain optical reaction information of sample B.

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