Fiber membrane for guiding periodontal tissue regeneration

Abstract

The utility model provides a fibrous membrane for guiding periodontal tissue regeneration, and relates to the technical field of medical instruments. Preparing a PLGA double-layer fiber membrane loaded with drug-loaded sodium hyaluronate particles through the cooperative work of electrostatic spinning and electrostatic spraying; the sodium hyaluronate particles can greatly improve the loading rate of hydrophilic anti-inflammatory antibacterial and bone regeneration promoting medicines; a PLGA compact layer in the PLGA double-layer fiber membrane is loaded with first sodium hyaluronate particles loaded with anti-inflammatory and antibacterial drugs, and when the first sodium hyaluronate particles make contact with gingival tissue, cell adhesion and permeation can be hindered, and the anti-inflammatory and antibacterial effects can be achieved; a PLGA loose layer in the PLGA double-layer fiber membrane carries second sodium hyaluronate particles loaded with a bone regeneration promoting medicine, and the second sodium hyaluronate particles can promote osteoblast adhesion and facilitate dentale regeneration when making contact with bone tissue.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, specifically to a fibrous membrane for guiding periodontal tissue regeneration. Background Technology

[0002] For patients with periodontal disease, the periodontal tissues are in a complex oral microenvironment for a long time and are attacked by a large number of pathogenic bacteria, which makes the treatment of periodontitis significantly different from traditional trauma treatment.

[0003] Post-periodontal surgery tissue healing often involves epithelial regeneration, which increases the risk of periodontal disease recurrence. Therefore, periodontal replacement materials have higher requirements than bone graft materials, and must possess certain mechanical properties to prevent epithelial cells from growing into the defective tissue.

[0004] Currently, commonly used treatment materials in periodontal surgery include bone powder and GTR-guided tissue regeneration membranes. The function of GTR membranes is to block epithelial tissue and allow periodontal ligament or osteoblast-derived cells to preferentially occupy the repair area for repair of defects. However, the application of existing GTR membranes as 3D scaffolds in tissue regeneration is often limited by drug loading efficacy. Electrospinning technology, as a commonly used high-tech method for preparing 3D scaffold structures, has been widely developed and applied. However, commonly used absorbable polymer medical materials typically use organic solvents as spinning solvents in electrospinning, which makes it difficult to improve the loading rate of many hydrophilic anti-inflammatory and antibacterial drugs. Utility Model Content

[0005] (a) Technical problems to be solved

[0006] To address the shortcomings of existing technologies, this invention provides a fibrous membrane for guiding periodontal tissue regeneration, solving the problem of low loading rate of hydrophilic drugs in fibrous membranes with a single structure for guiding periodontal tissue regeneration.

[0007] (II) Technical Solution

[0008] To achieve the above objectives, this utility model provides the following technical solution:

[0009] A fibrous membrane for guiding periodontal tissue regeneration, wherein the fibrous membrane is a PLGA bilayer fibrous membrane carrying sodium hyaluronate microparticles;

[0010] The PLGA bilayer fiber membrane consists of a dense PLGA layer and a loose PLGA layer.

[0011] The dense PLGA layer and the loose PLGA layer are integrally molded.

[0012] Preferably, the dense PLGA layer is used to contact the gingival tissue, and the loose PLGA layer is used to contact the bone tissue.

[0013] Preferably, the dense layer of PLGA is loaded with first sodium hyaluronate microparticles, and the porous layer of PLGA is loaded with second sodium hyaluronate microparticles; the first sodium hyaluronate microparticles are loaded with anti-inflammatory and antibacterial drugs, and the second sodium hyaluronate microparticles are loaded with bone regeneration-promoting drugs.

[0014] Preferably, the anti-inflammatory and antibacterial drugs loaded on the first sodium hyaluronate microparticles include: dexamethasone and antibiotics.

[0015] Preferably, the bone regeneration drug loaded on the second sodium hyaluronate microparticles includes: growth factors, recombinant BMP-2 protein, and parathyroid hormone.

[0016] Preferably, the fiber diameter of the PLGA dense layer is less than 1 μm, and the average pore size is 8-12 μm.

[0017] Preferably, the fiber diameter of the PLGA loose layer is 1.5-2.5 μm, and the average pore size is greater than 40 μm.

[0018] (III) Beneficial Effects

[0019] This invention provides a fibrous membrane for guiding periodontal tissue regeneration. Compared with the prior art, it has the following advantages:

[0020] In this invention, a PLGA bilayer fiber membrane loaded with drug-loaded sodium hyaluronate microparticles is prepared by electrospinning and electrospraying working in synergy. The sodium hyaluronate microparticles can significantly increase the loading rate of hydrophilic anti-inflammatory, antibacterial, and bone-regenerating drugs. The dense PLGA layer of the PLGA bilayer fiber membrane carries the first sodium hyaluronate microparticles loaded with anti-inflammatory and antibacterial drugs, which, upon contact with gingival tissue, can both inhibit cell adhesion and penetration and exert anti-inflammatory and antibacterial effects. The porous PLGA layer of the PLGA bilayer fiber membrane carries the second sodium hyaluronate microparticles loaded with bone-regenerating drugs, which, upon contact with bone tissue, can promote osteoblast adhesion and facilitate bone regeneration. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a schematic diagram of the exploded structure of the fiber membrane in an embodiment of this utility model;

[0023] The reference numerals in the figure are set as follows: PLGA dense layer 1, PLGA loose layer 2, first sodium hyaluronate microparticle 3, second sodium hyaluronate microparticle 4. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions in the embodiments of this utility model are described clearly and completely. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0025] This application provides a fibrous membrane for guiding periodontal tissue regeneration, thus solving the problem of low loading rate of hydrophilic drugs in fibrous membranes with a single structure for guiding periodontal tissue regeneration.

[0026] To better understand the above technical solutions, the following will provide a detailed explanation of the technical solutions in conjunction with the accompanying drawings and specific implementation methods.

[0027] Example:

[0028] like Figure 1 As shown, this utility model provides a fiber membrane for guiding periodontal tissue regeneration, wherein the fiber membrane is a PLGA bilayer fiber membrane loaded with sodium hyaluronate microparticles;

[0029] The PLGA bilayer fiber membrane comprises: a dense PLGA layer 1 and a loose PLGA layer 2;

[0030] The dense PLGA layer 1 and the loose PLGA layer 2 are integrally formed.

[0031] The dense PLGA layer 1 is used to contact the gingival tissue, and the loose PLGA layer 2 is used to contact the bone tissue.

[0032] like Figure 1 As shown, the dense PLGA layer 1 is loaded with first sodium hyaluronate microparticles 3, and the loose PLGA layer 2 is loaded with second sodium hyaluronate microparticles 4; the first sodium hyaluronate microparticles 3 are loaded with anti-inflammatory and antibacterial drugs, and the second sodium hyaluronate microparticles 4 are loaded with bone regeneration-promoting drugs.

[0033] The anti-inflammatory and antibacterial drugs loaded on the first sodium hyaluronate microparticle 3 include: dexamethasone and antibiotics.

[0034] The bone regeneration drugs loaded on the second sodium hyaluronate microparticle 4 include: growth factors, recombinant BMP-2 protein, and parathyroid hormone.

[0035] The fiber diameter of the PLGA dense layer 1 is less than 1 μm, and the average pore size is 8-12 μm.

[0036] The fiber diameter of the PLGA loose layer 2 is 1.5-2.5 μm, and the average pore size is greater than 40 μm.

[0037] In summary, compared with the prior art, the present invention has the following beneficial effects:

[0038] In this embodiment of the invention, a PLGA (polylactic acid) bilayer fiber membrane loaded with drug-loaded sodium hyaluronate microparticles is prepared by electrospinning and electrospraying working in synergy. The sodium hyaluronate microparticles can significantly increase the loading rate of hydrophilic anti-inflammatory, antibacterial, and bone regeneration-promoting drugs. The dense PLGA layer 1 of the PLGA bilayer fiber membrane carries the first sodium hyaluronate microparticle 3 loaded with anti-inflammatory and antibacterial drugs, which can both inhibit cell adhesion and penetration and play an anti-inflammatory and antibacterial role when in contact with gingival tissue. The loose PLGA layer 2 of the PLGA bilayer fiber membrane carries the second sodium hyaluronate microparticle 4 loaded with bone regeneration-promoting drugs, which can promote osteoblast adhesion and facilitate bone regeneration when in contact with bone tissue.

[0039] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0040] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A fibrous membrane for guiding periodontal tissue regeneration, characterized in that, The fiber membrane is a PLGA bilayer fiber membrane carrying sodium hyaluronate microparticles; The PLGA bilayer fiber membrane comprises: a dense PLGA layer (1) and a loose PLGA layer (2); The dense PLGA layer (1) and the loose PLGA layer (2) are integrally formed.

2. The fibrous membrane for guiding periodontal tissue regeneration as described in claim 1, characterized in that, The dense PLGA layer (1) is used to contact the gingival tissue, and the loose PLGA layer (2) is used to contact the bone tissue.

3. The fibrous membrane for guiding periodontal tissue regeneration as described in claim 1, characterized in that, The dense layer (1) of PLGA is loaded with a first sodium hyaluronate microparticle (3), and the loose layer (2) of PLGA is loaded with a second sodium hyaluronate microparticle (4); the first sodium hyaluronate microparticle (3) is loaded with anti-inflammatory and antibacterial drugs, and the second sodium hyaluronate microparticle (4) is loaded with bone regeneration drugs.

4. The fibrous membrane for guiding periodontal tissue regeneration as described in claim 3, characterized in that, The anti-inflammatory and antibacterial drugs loaded on the first sodium hyaluronate microparticle (3) include: dexamethasone and antibiotics.

5. The fibrous membrane for guiding periodontal tissue regeneration as described in claim 3, characterized in that, The bone regeneration drugs loaded on the second sodium hyaluronate microparticle (4) include: growth factors, recombinant BMP-2 protein and parathyroid hormone.

6. The fibrous membrane for guiding periodontal tissue regeneration as described in claim 1, characterized in that, The fiber diameter of the PLGA dense layer (1) is less than 1 μm, and the average pore size is 8-12 μm.

7. The fibrous membrane for guiding periodontal tissue regeneration as described in claim 1, characterized in that, The fiber diameter of the loose PLGA layer (2) is 1.5-2.5 μm, and the average pore size is greater than 40 μm.

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