32-hole extraction and purification magnetic frame

Abstract

The utility model relates to the technical field of extraction and purification, in particular to a 32-hole extraction and purification magnetic frame which comprises an aluminum alloy support, 32 PCR (polymerase chain reaction) tube holes are formed in the top of the aluminum alloy support and are divided into four groups, and each group comprises eight PCR tube holes; 32 cylindrical magnet mounting holes are obliquely formed in the two side walls of the aluminum alloy support, the 32 cylindrical magnet mounting holes are divided into four groups, each group comprises eight cylindrical magnet mounting holes, the lower portion of each PCR pipe hole corresponds to one cylindrical magnet mounting hole, and one cylindrical strong neodymium iron boron magnet is mounted in each cylindrical magnet mounting hole. According to the utility model, the magnet is kept vertical to the PCR tube by adopting an inclined magnet mode, so that the magnet strength is utilized to the maximum extent, the magnet adsorption efficiency is improved, and the magnetic bead adsorption time is shortened; and the aluminum alloy main body is adopted, so that the service life of the product is greatly prolonged.

Description

Technical Field

[0001] This utility model relates to the field of extraction and purification technology, specifically a 32-well magnetic rack for extraction and purification. Background Technology

[0002] Existing techniques for extracting and purifying magnetic racks commonly use 100 to 200 microliter PCR tubes. However, these racks have issues with the inability to fit tightly against the magnet, resulting in prolonged magnetic bead adsorption time during the experimental process. Furthermore, the back-and-forth shaking of the tube during liquid aspiration causes the magnetic beads to be attracted and lost, indirectly leading to sample loss. Utility Model Content

[0003] The purpose of this invention is to provide a 32-well magnetic extraction and purification rack to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a 32-well extraction and purification magnetic rack, comprising an aluminum alloy support, wherein the top of the aluminum alloy support has 32 PCR tube wells, which are divided into four groups of eight; the two side walls of the aluminum alloy support have 32 cylindrical magnet mounting holes, which are divided into four groups of eight, and each PCR tube well has a corresponding cylindrical magnet mounting hole below it, and each cylindrical magnet mounting hole has a cylindrical neodymium iron boron magnet installed inside it.

[0005] Preferably, the angle between the cylindrical magnet mounting hole and the horizontal bottom surface of the aluminum alloy bracket is 8.8°.

[0006] Preferably, the center lines of the PCR tube holes and the cylindrical magnet mounting holes intersect on the same plane.

[0007] Preferably, the center-to-center distance between the wells of each PCR tube in the same group is 9 mm.

[0008] Preferably, the center-to-center distance between the mounting holes of each cylindrical magnet in the same group is 9 mm.

[0009] Preferably, each of the PCR tube wells has an outwardly flared tapered chamfer at the top edge, which facilitates the insertion of the PCR tube.

[0010] Compared with existing technologies, the beneficial effects of this invention are as follows: During the implementation of this 32-well extraction and purification magnetic rack, the magnetic beads and the substances to be separated are mixed in a PCR tube. The mixed PCR tube is then directly inserted into the PCR tube wells of the aluminum alloy support. The magnetic beads are attracted by the strong magnetic field of the magnetic rack, and the magnetic beads in the PCR tube are attracted to one side of the centrifuge tube, transferring the unadsorbed liquid and achieving substance separation. Simultaneously, because the lower section of the PCR tube has a taper of 8.8°, the inclined cylindrical neodymium iron boron magnet maintains a perpendicular relationship with the outside of the PCR tube, allowing the conical surface of the PCR tube to fit tightly against the surface of the aluminum alloy support, improving adsorption strength and shortening adsorption time. Therefore, this extraction and purification magnetic rack uses an inclined magnet to keep the magnet perpendicular to the PCR tube, maximizing the use of magnet strength, improving magnet adsorption efficiency, and shortening the magnetic bead adsorption time. The use of an aluminum alloy body greatly extends the product's service life. Attached Figure Description

[0011] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0012] Figure 2 This is a three-dimensional exploded structural diagram of the present invention;

[0013] Figure 3 This is a schematic diagram of the oblique cross-sectional structure of this utility model;

[0014] Figure 4 This is a side view enlarged structural diagram of the present invention.

[0015] In the figure: 1. Aluminum alloy support; 2. PCR tube hole; 21. Tapered chamfer; 3. Cylindrical magnet mounting hole; 4. Cylindrical high-strength neodymium iron boron magnet. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, not all embodiments. In addition, the terms "first", "second", "third", "upper", "lower", "left", "right", etc. are used for descriptive purposes only and should not be construed as indicating or implying relative importance. At the same time, in the description of the present utility model, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present utility model.

[0017] This utility model provides a structure for a 32-well extraction and purification magnetic rack, as follows: Figure 1 as well as Figure 3 As shown, it includes an aluminum alloy support 1, with 32 PCR tube wells 2 on the top of the aluminum alloy support 1. The 32 PCR tube wells 2 are divided into four groups, with eight wells in each group. The center-to-center distance between the wells 2 in the same group is 9 mm. Each PCR tube well 2 has an outwardly expanding conical chamfer 21 at the top edge of the top. The conical chamfer 21 facilitates the insertion of PCR tubes.

[0018] During implementation, the PCR tube with a lower taper of 8.8° is inserted into the PCR tube hole 2 of the aluminum alloy support 1. The tapered chamfer 21 facilitates the insertion of the PCR tube during insertion.

[0019] Furthermore, such as Figure 2 as well as Figure 4 As shown, 32 cylindrical magnet mounting holes 3 are obliquely opened on both sides of the aluminum alloy support 1. The 32 cylindrical magnet mounting holes 3 are divided into four groups, with eight holes in each group. The center distance between the holes of each cylindrical magnet mounting hole 3 in the same group is 9mm. The angle between each cylindrical magnet mounting hole 3 and the horizontal bottom surface of the aluminum alloy support 1 is 8.8°. The cylindrical magnet mounting holes 3 and the center line of the PCR tube hole 2 intersect on the same plane. Each PCR tube hole 2 is located below a cylindrical magnet mounting hole 3. A cylindrical high-strength neodymium iron boron magnet 4 is installed inside each cylindrical magnet mounting hole 3.

[0020] During implementation, the magnetic beads inside the PCR tube are attracted by the strong magnetic field of the magnetic holder, and the magnetic beads inside the PCR tube are attracted to one side of the centrifuge tube, transferring the other unadsorbed liquids and achieving substance separation. At the same time, since the lower section of the PCR tube has a taper of 8.8°, the inclined cylindrical neodymium iron boron magnet 4 is kept perpendicular to the outside of the PCR tube, so that the conical surface of the PCR tube can be closely attached to the surface of the aluminum alloy support 1, which improves the adsorption intensity and shortens the adsorption time.

[0021] The principle of this invention for purifying substances is as follows: Various chemical substances are modified onto the surface of a paramagnetic material to prepare magnetic beads. These beads utilize the property that different chemical substances can bind to different target molecules. Once the target molecules are bound, the magnetic beads are adsorbed onto the side closest to the magnetic frame under the influence of a magnetic field. By discarding other reagents, the desired target molecules can be separated, achieving the purpose of extracting substances from or purifying substances from solution. This purification method is a common and well-known technique.

[0022] Working principle: The magnetic beads are mixed with the substances to be separated in the PCR tube. The mixed PCR tube is directly inserted into the PCR tube well 2 of the aluminum alloy support 1. The magnetic beads are attracted by the strong magnetic field of the magnetic support. The magnetic beads in the PCR tube are attracted to one side of the centrifuge tube, and the other unadsorbed liquids are transferred, thus realizing the separation of substances.

[0023] Meanwhile, since the lower section of the PCR tube has a taper of 8.8°, the inclined cylindrical neodymium iron boron magnet 4 maintains a perpendicular relationship with the outside of the PCR tube, allowing the conical surface of the PCR tube to fit tightly against the surface of the aluminum alloy support 1, thereby improving the adsorption intensity and shortening the adsorption time.

[0024] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A 32-well magnetic extraction and purification rack, comprising an aluminum alloy support (1), characterized in that: The top of the aluminum alloy support (1) is provided with 32 PCR tube holes (2), which are divided into four groups of eight. The two side walls of the aluminum alloy support (1) are provided with 32 cylindrical magnet mounting holes (3), which are divided into four groups of eight. Each PCR tube hole (2) is located below a cylindrical magnet mounting hole (3), and a cylindrical high-strength neodymium iron boron magnet (4) is installed inside each cylindrical magnet mounting hole (3).

2. The 32-well extraction and purification magnetic rack according to claim 1, characterized in that: The angle between the cylindrical magnet mounting hole (3) and the horizontal bottom surface of the aluminum alloy bracket (1) is 8.8°.

3. The 32-well extraction and purification magnetic rack according to claim 1, characterized in that: The center lines of the PCR tube hole (2) and the cylindrical magnet mounting hole (3) intersect on the same plane.

4. The 32-well extraction and purification magnetic rack according to claim 1, characterized in that: The distance between the centers of each well (2) in the same group is 9 mm.

5. A 32-well extraction and purification magnetic rack according to claim 1, characterized in that: The center-to-center distance between the holes (3) of each cylindrical magnet in the same group is 9 mm.

6. The 32-well extraction and purification magnetic rack according to claim 1, characterized in that: Each of the PCR tube wells (2) has an outwardly flared conical chamfer (21) at the top edge, which facilitates the insertion of the PCR tube.

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