Special culture plate for cell tubulation experiment

Abstract

The utility model relates to the technical field of cell culture, in particular to a special culture plate for a cell tubulation experiment, which comprises a cell tubulation culture plate, a plurality of groups of positioning rotating holes are uniformly formed in the cell tubulation culture plate, and a group of controllable dynamic culture trays are connected in each positioning rotating hole in an inserted manner. A plurality of groups of cell culture tubes for tube culture of cells are uniformly mounted in the top of the controllable dynamic culture tray, a rotary supporting bottom column is mounted in the middle of the bottom of the controllable dynamic culture tray, the bottom end of the rotary supporting bottom column and the inner bottom wall of the positioning rotary hole are positioned in a rotary magnetic attraction manner, and a circle of driven straight teeth are mounted in the middle of the rotary supporting bottom column; a group of driving straight teeth are rotationally arranged in the gear space, one side of each driving straight tooth is in meshed connection with a driven straight tooth inserted into the positioning rotating hole, and the bottom of each driving straight tooth is connected with a micro servo motor arranged in a groove in the bottom wall in the gear space. The culture plate realizes a use mode of integrated design of dynamic culture and static culture.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, specifically to a culture plate for cell tube formation experiments. Background Technology

[0002] Cell tube formation assay plates are experimental tools used to study biological processes such as angiogenesis, cell migration, and invasion. During cell culture, depending on cell type and available resources, dynamic or static culturing under simulated physiological conditions is employed, depending on the specific circumstances. However, existing cell tube formation assay plates are mostly simple static structures. When simulating a dynamic culture environment, bioreactors or microfluidic devices are needed, employing external forces such as shear forces to provide mechanical stimulation. While this method can simulate dynamic culture under certain requirements, it necessitates the use of external tools and equipment for each operation, making testing inconvenient, and requiring sterilization of the external equipment.

[0003] Therefore, in view of the above-mentioned problems, this technical solution proposes a special culture plate for cell tube formation experiments. Summary of the Invention

[0004] The purpose of this invention is to provide a special culture plate for cell tube formation experiments to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] This is a cell culture plate specifically designed for cell tube formation experiments. The plate contains multiple sets of evenly spaced rotating positioning holes. Each hole is connected to a controllable dynamic culture tray. Multiple cell culture tubes for cell tube formation are evenly installed on the top of each tray. A rotating support column is mounted at the center of the tray's bottom. The bottom of this column is magnetically positioned against the inner wall of the rotating positioning hole. A ring of driven spur teeth is installed in the center of the column. A gear space is located on one side of the inner wall of each positioning hole, containing a set of rotatable driving spur teeth. One side of these driving spur teeth meshes with the driven spur teeth inserted into the positioning hole. A gear shaft is installed in the middle of the active spur gear. The top of the gear shaft is rotatably connected to the top wall of the gear space, and the bottom is connected to a micro servo motor that is opened in the groove of the bottom wall of the gear space. After the rotating support column is inserted into the positioning rotation hole until the bottom end of the rotating support column is magnetically rotated to the bottom wall of the positioning rotation hole, the gear space and the active spur gear are synchronously meshed. At this time, the micro servo motor is started to drive the active spur gear to rotate, thereby driving the rotating support column to control the rotation of the controllable dynamic culture tray, thereby controlling the cells placed in the cell culture tube for dynamic culture. The micro servo motor can also be turned off as needed. At the same time, under the meshing limit of the active spur gear and the gear space, the controllable dynamic culture tray is kept in a static state for static culture.

[0007] Each set of micro servo motors is electrically connected to a micro motor button mounted on the outer circumferential wall of the cell tube culture plate via a connecting wire. Each set of micro servo motors corresponds to a set of micro motor buttons. That is, during actual cell tube culture, the corresponding controllable dynamic culture plate is rotated according to the number of cells cultured and the position of the corresponding controllable dynamic culture plate, thereby realizing the use mode of the integrated design of dynamic and static culture of this culture plate.

[0008] Compared with the prior art, the beneficial effects of this utility model are: by inserting the controllable dynamic culture plate into the positioning rotation hole and then selectively rotating it, and then utilizing the cell culture tube's function of tube culture, the dynamic and static culture functions of this culture plate are integrated, ensuring great convenience during use. At the same time, when conducting cell culture, different conditions can be cultured simultaneously according to the cell type culture requirements, thereby expanding the applicability of this culture plate. Attached Figure Description

[0009] Figure 1 A three-dimensional structural diagram of a culture plate specifically designed for cell tube formation experiments;

[0010] Figure 2 A top view of a culture plate specifically designed for cell tube formation experiments;

[0011] Figure 3 A schematic diagram of a partial structure connecting the controllable dynamic culture tray and the positioning rotating hole in a culture plate for cell tube formation experiments.

[0012] Figure 4 for Figure 3 A magnified structural diagram of A in the diagram.

[0013] The components include: a cell culture tube plate 10, a micro motor button 11, a controllable dynamic culture tray 12, a cell culture tube 13, a positioning rotation hole 14, a rotating support base column 15, a driven spur gear 16, a magnet plate 17, a magnetic metal block 18, a bottom hole 19, a gear space 20, an active spur gear 21, a gear shaft 22, a micro servo motor 23, connecting wires 24, and a top groove 25. Detailed Implementation

[0014] It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0015] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0016] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0017] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0018] Please see Figures 1-4A cell culture plate for tube formation experiments includes a cell culture plate 10. Multiple sets of positioning and rotating holes 14 are evenly distributed inside the cell culture plate 10. Each positioning and rotating hole 14 is connected to a controllable dynamic culture tray 12. Multiple sets of cell culture tubes 13 for cell tube formation are evenly installed inside the top of the controllable dynamic culture tray 12. A rotating support column 15 is installed at the bottom center of the controllable dynamic culture tray 12. The bottom end of the rotating support column 15 is magnetically positioned by rotational attraction to the inner wall of the positioning and rotating hole 14. A ring of driven spur teeth 16 is installed in the middle of the rotating support column 15. A gear space 20 is opened on one side of the inner wall of the positioning and rotating hole 14. A set of driving spur teeth 21 is rotatably arranged in the gear space 20. One side of the driving spur teeth 21 meshes with the driven spur teeth 16 inserted into the positioning and rotating hole 14. A gear shaft 22 is installed in the middle of the moving spur gear 21. The top of the gear shaft 22 is rotatably connected to the top wall of the gear space 20, and the bottom is connected to a micro servo motor 23 that is opened in the groove of the bottom wall of the gear space 20. After the rotating support column 15 is inserted into the positioning rotating hole 14 until the bottom end of the rotating support column 15 is magnetically rotated to the bottom wall of the positioning rotating hole 14, the gear space 20 and the moving spur gear 21 are synchronously meshed. At this time, the micro servo motor 23 is started to drive the moving spur gear 21 to rotate, thereby driving the rotating support column 15 to control the rotation of the controllable dynamic culture tray 12, thereby controlling the cells placed in the cell culture tube 13 to perform dynamic culture. The micro servo motor 23 can also be turned off as needed. At the same time, under the meshing limit of the moving spur gear 21 and the gear space 20, the controllable dynamic culture tray 12 is kept in a static state for static culture.

[0019] Each set of micro servo motors 23 is electrically connected to a micro motor button 11 mounted on the outer circumference of the cell tube culture plate 10 via a connecting wire 24. Each set of micro servo motors 23 corresponds to a set of micro motor buttons 11. That is, during actual cell tube culture, the corresponding controllable dynamic culture plate 12 is rotated or not according to the number of cells cultured and the position of the corresponding controllable dynamic culture plate 12, thereby realizing the use mode of the integrated design of dynamic culture and static culture of this culture plate.

[0020] In this embodiment of the invention, a magnet 17 is rotatably connected to the bottom end of the rotating support column 15, and a magnetic metal block 18 is installed on the bottom wall of the positioning rotating hole 14. After the rotating support column 15 is fully inserted into the positioning rotating hole 14, the magnet 17 and the magnetic metal block 18 are magnetically connected. At the same time, when the rotating support column 15 rotates, the magnet 17 is stationary relative to the magnetic metal block 18, ensuring that the rotating support column 15 rotates smoothly while maintaining stability in the vertical direction.

[0021] The upper and lower sides of the positioning rotation hole 14 are respectively set as a top groove 25 and a bottom hole 19. The bottom hole 19 is used for the insertion and positioning of the rotating support bottom column 15. The top groove 25 is used for the embedding and positioning of the controllable dynamic culture plate 12. At the same time, a sealing ring is wrapped around the circumferential outer wall of the top groove 25, and the sealing ring is slidably sealed to the inner wall of the top groove 25.

[0022] In one embodiment of the present invention, the internal structure of the cell culture tube 13 is designed as follows:

[0023] Double-layer structure: It usually consists of two layers, the upper layer is used for cell culture, and the lower layer can be added with culture medium or inducing factors.

[0024] Microporous membrane: The upper bottom layer has a microporous membrane that allows cells to pass through and form tubular structures.

[0025] Matrix coating: A matrix (such as Matrigel) is often pre-coated to mimic the extracellular environment and promote cell tube formation.

[0026] Here is a brief description of the cell culture tube 13: (1) Prepare a controllable dynamic culture tray 12 and insert it into the positioning rotating hole 14, thaw it and pre-coat it with matrix.

[0027] (2) Inoculate cells: Add the cell suspension to the upper layer.

[0028] (3) Culture: Culture under suitable conditions. Depending on the type of cell culture, choose static culture or dynamic culture, and observe cell behavior at the same time.

[0029] (4) Detection: Analyze the tube formation by microscopy or staining.

[0030] As a preferred embodiment of the present invention, the rotating support base column 15, driven spur gear 16, driving spur gear 21, and gear shaft 22 are all made of plastic material, which ensures stable connection and operation, while reducing the weight of the entire cell culture plate 10 and reducing production costs.

[0031] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention.

Claims

1. A culture plate specifically for cell tube formation experiments, characterized in that, The system includes a cell culture plate (10), which has multiple sets of positioning and rotating holes (14) evenly distributed inside. Each positioning and rotating hole (14) is connected to a set of controllable dynamic culture trays (12) in a plug-in manner. Multiple sets of cell culture tubes (13) for cell culture are evenly installed inside the top of the controllable dynamic culture trays (12). A rotating support column (15) is installed in the middle of the bottom of the controllable dynamic culture trays (12). The bottom end of the rotating support column (15) is magnetically positioned by rotating with the bottom wall of the positioning and rotating hole (14). A ring of driven spur teeth (16) is installed in the middle of the column (15). A gear space (20) is opened on one side of the inner wall of the positioning rotation hole (14). A set of active spur teeth (21) is rotatably arranged in the gear space (20). One side of the active spur teeth (21) meshes with the driven spur teeth (16) inserted into the positioning rotation hole (14). A gear shaft (22) is installed in the middle of the active spur teeth (21). The top of the gear shaft (22) is rotatably connected to the top wall of the gear space (20), and the bottom is connected to a miniature servo motor (23) opened in the groove of the bottom wall of the gear space (20). Each micro servo motor (23) is electrically connected to a micro motor button (11) mounted on the outer circumferential wall of the cell tube culture plate (10) via a connecting wire (24). Each micro servo motor (23) corresponds to a set of micro motor buttons (11).

2. The cell tube formation assay culture plate according to claim 1, characterized in that, A magnet (17) is rotatably connected to the bottom end of the rotating support column (15), and a magnetic metal block (18) is installed on the bottom wall of the positioning rotating hole (14).

3. The cell tube formation assay culture plate according to claim 2, characterized in that, The upper and lower sides of the positioning rotating hole (14) are respectively configured as a top groove (25) and a bottom hole (19).

4. The cell tube formation assay culture plate according to claim 3, characterized in that, A sealing ring is wrapped around the circumferential outer wall of the top groove (25), and the sealing ring is slidably sealed to the inner wall of the top groove (25).

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