Quantitative high-precision capillary electrophoresis apparatus

Abstract

The utility model discloses a quantitative high-precision capillary electrophoresis apparatus and relates to the field of biochemical detection.The quantitative high-precision capillary electrophoresis apparatus comprises an electrophoresis apparatus body, a mounting cavity is formed in the inner side of the electrophoresis apparatus body, a mounting frame is fixed in the mounting cavity, capillary tubes are fixed to the upper portion of the mounting frame through a plurality of clamps, and a mounting groove is formed in the side end of the electrophoresis apparatus body; and the groove bottom of the mounting groove is provided with two insertion holes communicating with the mounting cavity, a mounting block is arranged in the mounting groove, two electric heating plates are arranged on the mounting block, the electric heating plates are inserted into the insertion holes in the corresponding positions, and the two electric heating plates are located on the front side and the rear side of the capillary tube correspondingly. According to the electrophoresis apparatus, the capillary tube can be heated from the two sides of the capillary tube through the two electric heating plates, the heating effect is more uniform, an operator can replace the electric heating plates from the outside of the electrophoresis apparatus body when the electric heating plates are unstable in heating, and the operation difficulty is low.

Description

Technical Field

[0001] This application relates to the field of biochemical detection, and in particular to a quantitative high-precision capillary electrophoresis instrument. Background Technology

[0002] Capillary electrophoresis, a leading analytical technique, is widely used across various fields due to its high efficiency, sensitivity, speed, simple equipment, and broad applicability. It is a novel liquid-phase separation technique that uses capillaries as separation channels and a high-voltage DC electric field as the driving force. Capillary electrophoresis actually encompasses electrophoresis, chromatography, and their intersections, enabling analytical chemistry to move from the microliter to the nanoliter level and making single-cell and even single-molecule analysis possible.

[0003] For example, the capillary electrophoresis apparatus with patent number CN202021327269.8 has the following shortcomings in actual use:

[0004] The aforementioned device includes a capillary assembly containing a capillary tube. The cathode end of the capillary tube can be connected to the cathode reaction liquid, and the anode end of the capillary tube can be connected to the anodic reaction liquid. An anodic electrode is disposed in the anodic reaction liquid, and a current detection component is disposed between the anodic electrode and the high-voltage grounding wire to measure the capillary electrophoresis current. By measuring the capillary electrophoresis current, the high-voltage insulation of the instrument and the presence of air bubbles in the capillary liquid path can be detected, thus improving the detection accuracy of the capillary electrophoresis instrument. However, the heating component on the outside of the capillary tube may experience uneven heating after prolonged use. The heating component of the aforementioned device is difficult to replace, which is not conducive to maintaining uniform temperature in various parts of the capillary tube and reduces the detection accuracy. Summary of the Invention

[0005] To address the technical problem of the difficulty in replacing the heating components of capillary electrophoresis, this application provides a quantitative high-precision capillary electrophoresis apparatus.

[0006] The quantitative high-precision capillary electrophoresis apparatus provided in this application adopts the following technical solution:

[0007] A quantitative high-precision capillary electrophoresis apparatus includes an apparatus body. An installation cavity is provided on the inner side of the apparatus body, and a mounting frame is fixed within the cavity. A capillary tube is fixed above the mounting frame by several clips. An installation groove is provided on the side end of the apparatus body, and two insertion holes communicating with the installation cavity are provided at the bottom of the groove. An installation block is provided in the groove, and two heating plates are provided on the mounting block. The heating plates are inserted into corresponding insertion holes, and the two heating plates are located on the front and rear sides of the capillary tube, respectively.

[0008] By adopting the above technical solution, the two heating plates heat the capillary from both sides, resulting in a more uniform heating effect and effectively improving the detection accuracy. After long-term use, if the heating of the heating plates becomes unstable, the operator can pull the mounting block outward from the outside of the electrophoresis apparatus, thereby moving the two heating plates outward to replace them with new ones. The operation is simple and convenient for experimental personnel.

[0009] Preferably, the mounting block has two symmetrically distributed pressing pieces integrally formed on both sides, and the pressing pieces are provided with hooks on the outer side. The inner walls on both sides of the mounting groove are provided with slots, and the hooks are located inside the slots.

[0010] By adopting the above technical solution, the two hooks are locked inside the slots, which can prevent the mounting block and the heating plate from falling out during use or transportation, ensuring the stability of the experimental process and the safety of the transportation process. When it is necessary to remove the heating plate, the two pressing pieces can be pressed inward to make the hooks disengage from the slots, and the mounting block and the heating plate can be pulled out. The operation is simple.

[0011] Preferably, the outer side of the pressing piece is provided with an arc-shaped notch for pressing force.

[0012] By adopting the above technical solution, the arc-shaped notch makes it easier for operators to hold the installation block when it is pulled out, preventing slippage and improving the user experience of the equipment.

[0013] Preferably, the mounting block has two first electrical contacts symmetrically distributed vertically on its side wall near the heating plate for conducting electricity to the heating plate, and the bottom of the mounting groove has two second electrical contacts that abut against the first electrical contacts.

[0014] By adopting the above technical solution, the heating plate needs to be conductive to heat up. After the mounting block is installed, the two first electrical contacts and the two second electrical contacts make contact respectively, thus realizing the circuit loop and facilitating the power supply to the heating plate without the need for additional wiring.

[0015] Preferably, the mounting block has a sliding groove, and two lateral grooves are formed on both sides of the sliding groove. Two locking blocks are slidably arranged in the two lateral grooves, and a pressing block and a magnetic block are slidably arranged in the sliding groove. The pressing block is located below the two locking blocks and is in inclined contact with the locking blocks. The magnetic block is fixed below the pressing block. An electromagnet is fixed in the sliding groove and is located below the magnetic block.

[0016] By adopting the above technical solution, when the heating plate is powered on, the electromagnet is also powered on. At this time, the same magnetic poles of the electromagnet and the magnetic block are opposite each other, generating a repulsive force. This can push the magnetic block and the pressing block upward, causing the pressing block to move between the two locking blocks and press the two locking blocks outward. When the operator presses the pressing plate, it will be blocked by the two locking blocks, making the mounting block unable to be disassembled. This prevents the operator from removing the heating plate during the heating process, which would lead to inaccurate test data.

[0017] Preferably, the slide groove has an embedding groove, the width of which is greater than that of the slide groove, and the electromagnet is located inside the embedding groove. A removable cover plate is provided on the outside of the slide groove.

[0018] By adopting the above technical solution, the cover plate can be removed during installation, and then the electromagnet can be installed in the embedded groove. The two ends of the electromagnet are blocked, so it will not slide in the groove, thus achieving a fixing effect. Moreover, after the cover plate is removed, the card block, magnetic block, and squeezing block can be installed, making the installation process simpler and more efficient.

[0019] Preferably, the mounting bracket is provided with a plurality of temperature sensors, and the probes of the temperature sensors are oriented toward different parts of the capillary.

[0020] By adopting the above technical solution, the temperature sensor can monitor the temperature of various parts of the capillary in real time. When a large temperature difference occurs, an abnormality warning can be issued, which will facilitate timely maintenance by the operator.

[0021] Preferably, the end of the heating plate away from the mounting block is provided with a mounting chamfer.

[0022] By adopting the above technical solution, the chamfered corners can act as guides when the heating plate is inserted into the socket, reducing the difficulty of installation and preventing damage to the corners of the heating plate.

[0023] Preferably, the second electrical contact is an elastic metal sheet.

[0024] By adopting the above technical solution, the elastic metal sheet has an outward squeezing force, which can make a tighter contact with the first electrical contact, ensuring smooth circuit and preventing poor contact.

[0025] In summary, this application includes at least one of the following beneficial technical effects:

[0026] 1. After long-term use, if the heating of the electric heating plate becomes unstable, the operator can pull the mounting block outward from the outside of the electrophoresis apparatus, thereby moving the two electric heating plates outward to replace them with new ones. The operation is simple and convenient for experimental personnel.

[0027] 2. Two heating plates heat the capillary from both sides, resulting in more uniform heating and effectively improving detection accuracy. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the overall isometric structure of this application;

[0029] Figure 2 This is a schematic diagram of the internal structure of the mounting cavity in this application;

[0030] Figure 3 For this application Figure 2 Enlarged structural diagram at point A;

[0031] Figure 4 This is a schematic diagram of the structure of the heating plate in this application;

[0032] Figure 5 This is a schematic diagram of the mounting block in this application;

[0033] Figure 6 This is a schematic diagram of the disassembled structure of the mounting block in this application.

[0034] Reference numerals: 1. Electrophoresis apparatus body; 11. Mounting cavity; 12. Mounting bracket; 13. Capillary tube; 14. Temperature sensor;

[0035] 2. Mounting slot; 21. Insertion hole; 22. Card slot;

[0036] 3. Mounting block; 31. Pressing plate; 32. Hook; 33. Arc-shaped notch; 34. Slide groove; 35. Side groove; 36. Cover plate; 37. Embedded groove;

[0037] 4. Heating plate; 41. Chamfering installation;

[0038] 5. First electrical contact; 6. Second electrical contact; 7. Clamping block; 8. Pressing block; 9. Magnetic block; 10. Electromagnet. Detailed Implementation

[0039] The following is in conjunction with the appendix Figure 1-5 This application will be described in further detail.

[0040] This application discloses a quantitative high-precision capillary electrophoresis apparatus.

[0041] Reference Figure 1-4A quantitative high-precision capillary electrophoresis apparatus includes an apparatus body 1. An installation cavity 11 is provided inside the apparatus body 1. A mounting frame 12 is fixed in the installation cavity 11. A capillary tube 13 is fixed above the mounting frame 12 by several clips. Several temperature sensors 14 are provided on the mounting frame 12 to detect the temperature of different parts of the capillary tube 13. An installation groove 2 is provided on the side end of the apparatus body 1. Two insertion holes 21 communicating with the installation cavity 11 are provided at the bottom of the installation groove 2. An installation block 3 is provided in the installation groove 2. Two... The first electrical contact 5 is symmetrically distributed vertically. The bottom of the mounting groove 2 is provided with two second electrical contacts 6, which are elastic metal sheets. The second electrical contacts 6 abut against the first electrical contacts 5. The mounting block 3 is provided with two heating plates 4, which are inserted into the corresponding insertion holes 21. The two heating plates 4 are located on the front and rear sides of the capillary tube 13, respectively. The first electrical contact 5 and the second electrical contact 6 supply power to the heating plates 4. The end of the heating plate 4 away from the mounting block 3 is provided with a mounting chamfer 41. The first electrical contact 5 and the second electrical contact 6 are both made of copper.

[0042] With the above settings, the first electrical contact 5 and the second electrical contact 6 energize the heating plate 4. The elastic metal sheet has an outward squeezing force, which can make a tighter contact with the first electrical contact 5, ensuring smooth circuit and preventing poor contact. The two heating plates 4 heat the capillary tube 13 from both sides, resulting in a more uniform heating effect and effectively improving detection accuracy. When the temperature sensor 14 detects a large temperature difference in different parts of the capillary tube 13, it indicates that the heating of the heating plate 4 is unstable. The operator can pull the mounting block 3 outward from the outside of the electrophoresis apparatus body 1, thereby moving the two heating plates 4 outward to replace the new heating plate 4. The operation is simple and convenient for the experimenter. Moreover, when inserting a new heating plate 4, the installation chamfer 41 can play a guiding role, reducing the installation difficulty and preventing damage to the corners of the heating plate 4.

[0043] Reference Figure 3 , 5 The mounting block 3 has two integrally formed pressing pieces 31 on both sides, and the two pressing pieces 31 are symmetrically distributed and can be pressed inward. The outer side of the pressing piece 31 is provided with an arc-shaped notch 33 for pressing force, and the outer side of the pressing piece 31 is provided with a hook 32. The inner walls on both sides of the mounting groove 2 are provided with a groove 22 that matches the hook 32, and the hook 32 is locked inside the groove 22.

[0044] With the above-mentioned setup, the two hooks 32 are secured inside the slots 22, preventing the mounting block 3 and the heating plate 4 from falling out during use or transport, thus ensuring the stability of the experimental process and the safety of the transport process. When the heating plate 4 needs to be removed, the two pressing pieces 31 can be pressed inward through the arc-shaped notch 33, causing the hooks 32 to disengage from the slots 22, allowing the mounting block 3 and the heating plate 4 to be pulled out. The operation is simple, and the arc-shaped notch 33 can prevent slippage when removing the mounting block 3, improving the user experience of the equipment.

[0045] refer to Figure 6 The mounting block 3 has a sliding groove 34 and an embedding groove 37 with a width greater than the sliding groove 34. A detachable cover plate 36 is provided on the outside of the sliding groove 34. The cover plate 36 is connected to the mounting block 3 by bolts. Two symmetrically distributed side grooves 35 are provided on both sides of the sliding groove 34. A locking block 7 is slidably arranged in the side groove 35. A pressing block 8 and a magnetic block 9 are slidably arranged in the sliding groove 34. The pressing block 8 is located below the two locking blocks 7. A first chamfer is provided on the corner of the locking block 7 near the pressing block 8. A second chamfer is provided on both sides of the upper end of the pressing block 8. The first chamfer and the second chamfer fit together. The magnetic block 9 is fixed below the pressing block 8. An electromagnet 10 is fixed in the embedding groove 37 and is located below the magnetic block 9.

[0046] With the above settings, when installing components in the sliding groove 34 and the embedding groove 37, the cover plate 36 can be removed, making installation more convenient. Moreover, the electromagnet 10 is more stable in the embedding groove 37 and will not slide. When the heating plate 4 is energized, the electromagnet 10 will also be energized. At this time, the same magnetic poles of the electromagnet 10 and the magnetic block 9 are opposite each other, generating a repulsive force between them. This can push the magnetic block 9 and the pressing block 8 upward, causing the pressing block 8 to move between the two locking blocks 7 and press the two locking blocks 7 outward. When the operator presses the pressing piece 31, it will be blocked by the two locking blocks 7, preventing the mounting block 3 from being disassembled. This prevents the operator from removing the heating plate 4 during the heating process, which would lead to inaccurate test data. When the electromagnet 10 is de-energized, the material properties of the electromagnet 10 itself will generate an attractive force between it and the magnetic block 9. At this time, the magnetic block 9 will drive the pressing block 8 to move downward and away from the position between the two locking blocks 7. At this time, the operator can press the pressing piece 31 inward and disassemble the mounting block 3.

[0047] The implementation principle of a quantitative high-precision capillary electrophoresis apparatus according to an embodiment of this application is as follows: The first electrical contact 5 and the second electrical contact 6 energize the heating plate 4. The two heating plates 4 heat the capillary 13 from both sides, resulting in more uniform heating and effectively improving detection accuracy. Furthermore, the two hooks 32 are secured inside the slots 22, preventing the mounting block 3 and the heating plate 4 from detaching during use or transport, ensuring the stability of the experimental process and the safety of the transport process. In addition, when the heating plate 4 is energized, the electromagnet 10 is also energized. At this time, the same magnetic poles of the electromagnet 10 and the magnetic block 9 are opposite each other, generating a repulsive force that pushes the magnetic block 9 and the squeezing block 8 upwards, causing the squeezing block 8 to move between the two locking blocks 7, squeezing the two locking blocks 7 outwards. When the operator presses the pressing plate 31, it will be blocked by the two locking blocks 7, preventing the mounting block 3 from being disassembled. This prevents the operator from removing the heating plate 4 during the heating process, which would lead to inaccurate test data. When the temperature sensor 14 detects a large temperature difference in different parts of the capillary tube 13, it indicates that the heating of the heating plate 4 is unstable. The operator can press the two pressing plates 31 inward through the arc-shaped notch 33, causing the locking hook 32 to disengage from the locking groove 22. This allows the mounting block 3 and the heating plate 4 to be pulled outward, thereby moving the two heating plates 4 outward to replace the new heating plate 4. The operation is relatively simple and convenient for the experimenter. Moreover, when inserting the new heating plate 4, the installation chamfer 41 can play a guiding role, reducing the installation difficulty and preventing damage to the corners of the heating plate 4.

[0048] In summary, this device can heat the capillary 13 from both sides using two heating plates 4, resulting in more uniform heating. Furthermore, if the heating plates 4 become unstable, the operator can replace them from the outside of the electrophoresis apparatus 1, making the operation easier and improving the detection accuracy.

[0049] The above are all preferred embodiments of this application and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A quantitative high-precision capillary electrophoresis apparatus, comprising an electrophoresis apparatus body (1), wherein an installation cavity (11) is provided on the inner side of the electrophoresis apparatus body (1), a mounting frame (12) is fixed in the installation cavity (11), and a capillary tube (13) is fixed above the mounting frame (12) by a plurality of clips, characterized in that: The electrophoresis apparatus body (1) has a mounting groove (2) on its side end, and the bottom of the mounting groove (2) has two insertion holes (21) that connect to the mounting cavity (11). The mounting groove (2) is provided with a mounting block (3), and the mounting block (3) is provided with two electric heating plates (4). The electric heating plates (4) are inserted into the corresponding insertion holes (21), and the two electric heating plates (4) are located on the front and rear sides of the capillary tube (13) respectively.

2. The high-precision capillary electrophoresis apparatus for quantitative analysis according to claim 1, characterized in that: The mounting block (3) has two symmetrically distributed pressing pieces (31) integrally formed on both sides, and the pressing pieces (31) are provided with hooks (32) on the outer side. The mounting groove (2) is provided with slots (22) on both sides of the inner wall, and the hooks (32) are located inside the slots (22).

3. The high-precision capillary electrophoresis apparatus for quantitative analysis according to claim 2, characterized in that: The outer side of the pressing piece (31) is provided with an arc-shaped notch (33) for pressing force.

4. The high-precision capillary electrophoresis apparatus as claimed in claim 1, wherein: The mounting block (3) has two first electrical contacts (5) symmetrically distributed on its side wall near the heating plate (4) for conducting electricity to the heating plate (4). The bottom of the mounting groove (2) has two second electrical contacts (6) that abut against the first electrical contacts (5).

5. The high-precision capillary electrophoresis apparatus as claimed in claim 1, wherein: The mounting block (3) has a sliding groove (34) with two side grooves (35) on both sides. Two locking blocks (7) are slidably arranged in the two side grooves (35). A pressing block (8) and a magnetic block (9) are slidably arranged in the sliding groove (34). The pressing block (8) is located below the two locking blocks (7) and is in inclined contact with the locking blocks (7). The magnetic block (9) is fixed below the pressing block (8). An electromagnet (10) is fixed in the sliding groove (34) and is located below the magnetic block (9).

6. The high-precision capillary electrophoresis apparatus for quantitative analysis according to claim 5, characterized in that: An embedding groove (37) is provided in the slide groove (34). The width of the embedding groove (37) is greater than that of the slide groove (34), and the electromagnet (10) is located inside the embedding groove (37). A detachable cover plate (36) is provided on the outside of the slide groove (34).

7. The high-precision capillary electrophoresis apparatus as claimed in claim 1, wherein: The mounting bracket (12) is provided with several temperature sensors (14), and the probes of the temperature sensors (14) are oriented toward different parts of the capillary tube (13).

8. The high-precision capillary electrophoresis apparatus as claimed in claim 1, wherein: The heating plate (4) has a chamfer (41) at the end away from the mounting block (3).

9. The high-precision capillary electrophoresis apparatus for quantitative analysis according to claim 4, characterized in that: The second electrical contact (6) is an elastic metal sheet.

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