Gene sequencing kit bottom box convenient to position
By introducing a positioning and sealing mechanism into the gene sequencing kit cassette, the problem of effective cassette positioning is solved, ensuring rapid and accurate sample loading and the stability of sequencing results, and avoiding detection interference caused by shaking and contamination.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- CHINA JILIANG UNIV
- Filing Date
- 2025-05-22
- Publication Date
- 2026-04-24
AI Technical Summary
Existing gene sequencing kit cassettes are difficult to position effectively, leading to prolonged detection processes and positioning errors that affect the accuracy and stability of sequencing results.
The system employs positioning and sealing mechanisms, including components such as telescopic rods, clamping arms, return springs, sealing gaskets, and limit blocks, to ensure precise docking between the base box and the testing instrument and to form a sealed space, preventing shaking and contamination.
It enables rapid and accurate docking between the cassette and the detection instrument, reduces the risk of abnormal sample loading and contamination, and ensures the quality and integrity of gene sequencing data.
Smart Images
Figure CN224159647U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biomedical technology, and in particular to a base box for a convenient localization gene sequencing reagent kit. Background Technology
[0002] In pharmaceutical oncology gene sequencing scenarios, the gene sequencing kit cartridge plays a crucial role. Before administering medication to cancer patients, doctors need to use gene sequencing to determine whether the patient has specific gene mutations, thereby screening for suitable targeted drugs. The cartridge can accurately place the patient's tumor tissue or blood sample, ensuring the stability of the sample during transportation, storage, and testing, and avoiding cross-contamination or degradation of the sample due to shaking.
[0003] In the existing technology, some reagent kit boxes typically employ a precise grid-like positioning structure. Each positioning slot is precisely designed according to the standard size of the tumor gene detection reagent tube, which can firmly fix the reagent tube and ensure its stability during operation and transportation. Some boxes are also equipped with a cushioning protection device, which can effectively absorb vibration and prevent the reagent tube from being damaged by collision, thus ensuring the integrity and effectiveness of the tumor gene sequencing reagent.
[0004] However, in practical use, some gene sequencing reagent kit cartridges are difficult to position effectively, which seriously affects the detection process. In actual operation, it is difficult for experimenters to place them quickly and accurately in the designated position on the instrument, resulting in repeated adjustments, significantly prolonging sample loading time and reducing detection efficiency. At the same time, positioning deviations can easily cause poor contact between the reagent kit and the instrument detection module, interfering with the accuracy and stability of sequencing results. To address the above problems, a gene sequencing reagent kit cartridge that is easy to position is proposed. Utility Model Content
[0005] To overcome the above shortcomings, this utility model provides a convenient positioning gene sequencing reagent kit bottom, aiming to improve the problem that some gene sequencing reagent kit bottoms in the prior art are difficult to effectively position.
[0006] To achieve the above objectives, the present invention adopts the following technical solution:
[0007] A convenient positioning gene sequencing reagent kit bottom box includes a bottom box, a reagent kit fixedly connected inside the bottom box, a fixing plate fixedly connected inside the bottom box, a follower block fixedly connected outside the bottom box, a top cover rotatably connected to the outside of the follower block, a positioning mechanism fixedly connected to the bottom of the fixing plate, and a sealing mechanism fixedly connected to the outside of the bottom box.
[0008] The positioning mechanism includes a telescopic rod, which is externally fixedly connected to the outside of the fixed plate. An elastic component is externally fixedly connected to the outside of the fixed plate. A connecting ring is slidably connected to the bottom of the base box. Multiple support plates are externally fixedly connected to the outside of the connecting ring. A follower plate is rotatably connected to the outside of the support plate. A fixed rod is externally fixedly connected to the outside of the follower plate. A clamping arm is externally fixedly connected to the outside of the fixed rod. A limit spring is externally fixedly connected to the outside of the clamping arm.
[0009] As a further description of the above technical solution:
[0010] The elastic component includes a return spring, and a follower block two is fixedly connected to the outside of the return spring. The return spring is also fixedly connected to the outside of the fixed plate.
[0011] As a further description of the above technical solution:
[0012] The sealing mechanism includes a sealing gasket, a connecting rod that is slidably connected inside the sealing gasket, a connecting ring that is fixedly connected outside the connecting rod, and the sealing gasket that is detachably connected to the outside of the base box.
[0013] As a further description of the above technical solution:
[0014] The connecting rod has a limit hole inside, and the bottom box is fixedly connected to a limit spring.
[0015] As a further description of the above technical solution:
[0016] The second limiting spring is externally fixedly connected to a follower rod, the follower rod is externally fixedly connected to a limiting block, and the limiting block is externally slidably connected to the inside of the connecting rod.
[0017] As a further description of the above technical solution:
[0018] A U-shaped plate is fixedly connected to the outside of the bottom box, and a clamping plate is rotatably connected to the outside of the U-shaped plate;
[0019] As a further description of the above technical solution:
[0020] A positioning rod is fixedly connected to the outside of the card plate, and a tension spring is fixedly connected to the outside of the card plate.
[0021] As a further description of the above technical solution:
[0022] One end of the tension spring is fixedly connected to the outside of the card plate, and the other end of the tension spring is fixedly connected to the outside of the top cover.
[0023] This utility model has the following beneficial effects:
[0024] 1. In this utility model, by placing the bottom box on the sequencing mounting base, the connecting ring drives the follower block two to move, which in turn drives the telescopic rod to move, and then the follower block two drives the clamping arm to move, thereby ensuring that the bottom box and the detection instrument are accurately connected, avoiding abnormal sample loading due to positional deviation, and ensuring the stability of the bottom box during the detection process, reducing uneven reagent mixing or sample loss caused by shaking, and ensuring the quality of gene sequencing data.
[0025] 2. In this utility model, by fixing the sealing gasket to the outside of the bottom box, the connecting ring drives the connecting rod to move, which in turn drives the follower rod to move, and then the limiting block moves inside the limiting hole, thereby achieving a seal on the bottom box. This prevents the intrusion of external dust and moisture, avoids reagent deterioration and failure, eliminates contact between the sample and external substances, prevents contamination, and ensures accurate sequencing results. Attached Figure Description
[0026] Figure 1 This is a three-dimensional schematic diagram of the bottom box of a gene sequencing reagent kit for easy positioning proposed in this utility model;
[0027] Figure 2 This is a schematic diagram of the follower block 1 of the bottom box of a convenient positioning gene sequencing reagent kit proposed in this utility model;
[0028] Figure 3 This is a schematic diagram of the sealing gasket structure of the bottom box of a convenient positioning gene sequencing reagent kit proposed in this utility model;
[0029] Figure 4 for Figure 2 Enlarged view of point A in the middle;
[0030] Figure 5 for Figure 3 Enlarged view of point B in the middle.
[0031] Legend:
[0032] 1. Base box; 2. Reagent kit; 3. Fixing plate; 4. Follower block one; 5. Top cover; 6. Telescopic rod; 7. Return spring; 8. Follower block two; 9. Connecting ring; 10. Support plate; 11. Follower plate; 12. Fixing rod; 13. Clamping arm; 14. Limiting spring one; 15. Sealing gasket; 16. Connecting rod; 17. Connecting ring; 18. Limiting hole; 19. Limiting spring two; 20. Follower rod; 21. Limiting block; 22. U-shaped plate; 23. Clamping plate; 24. Positioning rod; 25. Tension spring. Detailed Implementation
[0033] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0034] Reference Figure 1 , Figure 2 , Figure 4 This utility model provides an embodiment of a positioning-friendly gene sequencing reagent kit bottom box, including a bottom box 1. A reagent kit 2 is fixedly connected inside the bottom box 1. The bottom box 1 is generally in the shape of a regular cuboid with rounded corners to ensure structural strength and prevent bumps during use. The bottom box 1 has a hollow interior, and its size is adapted to the height of the rectangular reagent kit 2. The inner wall is provided with anti-slip texture. It is tightly fixed to the reagent kit 2 through an embedded slot to ensure that the reagent kit 2 is placed stably inside the bottom box 1 and will not affect the stability of the reagent due to shaking. A fixing plate 3 is fixedly connected inside the bottom box 1. The fixing plate 3 not only provides reliable support for other functional components, but also plays a certain role in electromagnetic shielding to reduce the impact of external electromagnetic interference on the precision reagents inside the reagent kit 2. A follower block 4 is fixedly connected to the outside of the bottom box 1. A top cover 5 is rotatably connected to the outside of the follower block 4. A positioning mechanism is fixedly connected to the bottom of the fixing plate 3. A sealing mechanism is fixedly connected to the outside of the bottom box 1.
[0035] The positioning mechanism includes a telescopic rod 6, one end of which is firmly fixed to the bottom of the fixed plate 3, allowing for flexible extension and retraction along the axial direction. The telescopic rod 6 is externally fixedly connected to the outside of the fixed plate 3, and an elastic component is externally fixedly connected to the outside of the fixed plate 3. A connecting ring 9 is slidably connected to the bottom of the base box 1, and multiple support plates 10 are externally fixedly connected to the outside of the connecting ring 9. A follower plate 11 is rotatably connected to the outside of the support plate 10, and a fixed rod 12 is externally fixedly connected to the outside of the follower plate 11. A clamping arm 13 is externally fixedly connected to the outside of the fixed rod 12, and a limit spring 14 is externally fixedly connected to the outside of the clamping arm 13. In the non-working state, the limit spring 14 is in a naturally extended state, keeping the clamping arm 13 at an opening angle of about 30 degrees.
[0036] When the base box 1 is positioned, the clamping arm 13 can retract inward under external pressure to tightly clamp the positioning column of the instrument and achieve stable fixation. The elastic component includes a return spring 7. When the base box 1 is subjected to external pressure, the return spring 7 will undergo elastic deformation, driving the follower block 8 to move up and down along the surface of the fixed plate 3. After the pressure is removed, the base box 1 is quickly reset to the initial state by relying on the elastic restoring force of the spring. The follower block 8 is fixedly connected to the outside of the return spring 7, and the outside of the fixed plate 3 is fixedly connected to the outside of the return spring 7.
[0037] Reference Figure 2 , Figure 3 , Figure 5 The sealing mechanism includes a sealing gasket 15, which is the core and has a rounded semi-circular cross-section. It can tightly fit the contact surface between the top cover 5 and the bottom box 1. A connecting rod 16 is slidably connected inside the sealing gasket 15, and a connecting ring 17 is fixedly connected outside the connecting rod 16. The sealing gasket 15 is detachably connected to the outside of the bottom box 1. The outer edge of the sealing gasket 15 has a protruding buckle structure that interlocks with the groove on the top edge of the bottom box 1 to achieve a detachable connection, which facilitates the replacement and maintenance of the sealing gasket 15. A limit hole 18 is opened inside the connecting rod 16. A limit spring 19 is fixedly connected to the outside of the bottom box 1. A follower rod 20 is fixedly connected to the outside of the limit spring 19. The follower rod 20 can slide flexibly along the axis under the action of the spring. The follower rod 20 is sleeved on the outside of the limit spring 19. A limit block 21 is fixedly connected to the outside of the follower rod 20. The limit block 21 is slidably connected to the inside of the connecting rod 16. A U-shaped plate 22 is fixedly connected to the outside of the bottom box 1.
[0038] The U-shaped plate 22 is rotatably connected to the rectangular clamping plate 23 via a transverse pivot, allowing the clamping plate 23 to swing around the pivot. A cylindrical positioning rod 24 is vertically fixed to the bottom of the clamping plate 23. When the top cover 5 is gradually pressed down, the clamping plate 23 is squeezed by the top cover 5 and rotates around the pivot. After the positioning rod 24 is inserted into the positioning hole, the tension spring 25 is further stretched, generating a strong pulling force, which makes the clamping plate 23 tightly fasten the top cover 5. At the same time, it drives the sealing gasket 15 to fit tightly, forming an efficient sealing space, isolating external impurities and moisture, and ensuring the safety of gene sequencing samples. The clamping plate 23 is rotatably connected to the outside of the U-shaped plate 22, the positioning rod 24 is fixedly connected to the outside of the clamping plate 23, and the tension spring 25 is fixedly connected to the outside of the clamping plate 23. One end of the tension spring 25 is fixedly connected to the outside of the clamping plate 23, and the other end of the tension spring 25 is fixedly connected to the outside of the top cover 5.
[0039] Working principle: By placing the bottom box 1 on the sequencing mount, the telescopic rod 6 extends from the bottom of the fixing plate 3. At the same time, the connecting ring 9 at the bottom of the bottom box 1 can slide, thereby driving the support plate 10 and the connected follower plate 11 to rotate, so that the clamping arm 13 on the fixing rod 12 aligns with the instrument positioning column. Under the action of external pressure, the clamping arm 13, which is in the open state, overcomes the elastic force of the limiting spring 14 and retracts inward to clamp the positioning column. When the bottom box 1 is squeezed by external force, the reset spring 7 outside the fixing plate 3 undergoes elastic deformation, pushing the follower block 8 to move along the surface of the fixing plate 3.
[0040] By sliding the top cover 5 along the raised guide rail of the follower block 4 to close, the clamping plate 23 rotates around the U-shaped plate 22 under the pressure of the top cover 5, so that the positioning rod 24 is inserted into the positioning hole of the top cover 5. At the same time, the tension spring 25 in a stretched state further tightens the clamping plate 23, which in turn causes the connecting rod 16 to slide in the sealing gasket 15, so that the limiting block 21 is inserted into the limiting hole 18 under the action of the limiting spring 19, compressing the sealing gasket 15 and making the sealing gasket 15 tightly fit the contact surface between the top cover 5 and the bottom box 1 to form a sealed space.
[0041] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A kit box for easy localization gene sequencing, comprising a kit box (1), characterized in that: The reagent kit (2) is fixedly connected inside the bottom box (1), the fixing plate (3) is fixedly connected inside the bottom box (1), the follower block (4) is fixedly connected outside the bottom box (1), the top cover (5) is rotatably connected outside the follower block (4), the positioning mechanism is fixedly connected to the bottom of the fixing plate (3), and the sealing mechanism is fixedly connected to the outside of the bottom box (1). The positioning mechanism includes a telescopic rod (6), which is externally fixedly connected to the outside of the fixed plate (3). An elastic component is externally fixedly connected to the outside of the fixed plate (3). A connecting ring (9) is slidably connected to the bottom of the bottom box (1). Multiple support plates (10) are externally fixedly connected to the outside of the connecting ring (9). A follower plate (11) is rotatably connected to the outside of the support plate (10). A fixed rod (12) is externally fixedly connected to the outside of the follower plate (11). A clamping arm (13) is externally fixedly connected to the outside of the fixed rod (12). A limit spring (14) is externally fixedly connected to the outside of the clamping arm (13).
2. The easy-to-position gene sequencing reagent kit chassis according to claim 1, characterized in that: The elastic component includes a return spring (7), and a follower block (8) is fixedly connected to the outside of the return spring (7). The return spring (7) is fixedly connected to the outside of the fixed plate (3).
3. The easy-to-position gene sequencing reagent kit chassis according to claim 2, characterized in that: The sealing mechanism includes a sealing gasket (15), a connecting rod (16) is slidably connected inside the sealing gasket (15), a connecting ring (17) is fixedly connected outside the connecting rod (16), and the sealing gasket (15) is detachably connected to the outside of the bottom box (1).
4. The easy-to-position gene sequencing reagent kit chassis according to claim 3, characterized in that: The connecting rod (16) has a limiting hole (18) inside, and the bottom box (1) is fixedly connected to a limiting spring (19).
5. The easy-to-position gene sequencing reagent kit chassis according to claim 4, characterized in that: The limiting spring 2 (19) is externally fixedly connected to a follower rod (20), the follower rod (20) is externally fixedly connected to a limiting block (21), and the limiting block (21) is externally slidably connected to the inside of the connecting rod (16).
6. The easy-to-position gene sequencing reagent kit chassis according to claim 1, characterized in that: The bottom box (1) is fixedly connected to the outside of a U-shaped plate (22), and the U-shaped plate (22) is rotatably connected to a clamping plate (23).
7. The easy-to-position gene sequencing reagent kit chassis according to claim 6, characterized in that: The card plate (23) is externally fixedly connected to a positioning rod (24), and the card plate (23) is externally fixedly connected to a tension spring (25).
8. The easy-to-position gene sequencing reagent kit chassis according to claim 7, characterized in that: One end of the tension spring (25) is fixedly connected to the outside of the card plate (23), and the other end of the tension spring (25) is fixedly connected to the outside of the top cover (5).