Reaction kettle for producing mycotoxin degrading enzyme

Abstract

The utility model relates to the technical field of enzyme substance preparation, in particular to a reaction kettle for producing mycotoxin degrading enzyme, which comprises a reaction kettle body, the ventilation stirring mechanism is arranged at the vertical center line in the reaction kettle body; the air inflation mechanism is mounted on the reaction kettle body, and the air inflation mechanism is used for inputting oxygen to the ventilation stirring mechanism; the driving mechanism is mounted on the reaction kettle body, and one end of the driving mechanism drives the ventilation stirring mechanism to rotate; according to the reaction kettle disclosed by the utility model, high-pressure gas is input through the axis, bubbles are crushed into micron-sized bubbles through rotating centrifugal force, and the bubbles are guided to be uniformly distributed along the axial direction through dispersed conical holes, so that local gas accumulation is avoided, and the foaming rate of fermentation liquor is reduced; the stirring shear force in the rotating process of the stirring shaft can directly break bubbles, so that the thalli are prevented from being damaged by local peroxidation.

Description

Technical Field

[0001] This utility model relates to the field of enzyme preparation technology, and in particular to a reaction vessel for producing fungal toxin degrading enzymes. Background Technology

[0002] Mycotoxin degrading enzymes are enzymes that can degrade different types of mycotoxins, breaking them down into non-toxic substances to ensure food safety. Currently, mycotoxin degrading enzymes and other substances have broad application prospects in food processing and storage, and are also one of the important means to ensure food quality and safety. It is necessary to use a reaction vessel to produce mycotoxin degrading enzymes.

[0003] Existing reactors have some shortcomings in the preparation of mycotoxin degrading enzymes: the mycotoxin degrading enzyme producing strains (such as Aspergillus, Trichoderma, etc.) are mostly aerobic microorganisms, and the cell metabolism requires continuous dissolved oxygen (DO concentration ≥ 30% saturation). Oxygen deficiency will lead to mycelial autolysis and enzyme production stagnation, but directly introducing oxygen can easily lead to cell peroxidation.

[0004] To address these issues, those skilled in the art have proposed a reaction vessel for the production of fungal toxin-degrading enzymes. Utility Model Content

[0005] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the present invention.

[0006] In view of the problem that directly introducing oxygen into the reaction vessel in the above or existing technologies can easily lead to bacterial peroxidation, this utility model is proposed.

[0007] Therefore, the purpose of this invention is to provide a reaction vessel for the production of fungal toxin degrading enzymes.

[0008] To solve the above-mentioned technical problems, this utility model provides the following technical solution: a reaction vessel for producing fungal toxin degrading enzymes, comprising, a reaction vessel body;

[0009] A ventilation and stirring mechanism is installed at the vertical centerline inside the reactor body;

[0010] An aeration mechanism is installed on the reactor body and is used to supply oxygen to the aeration and stirring mechanism.

[0011] A drive mechanism, mounted on the reactor body, one end of which drives the rotation of the aeration and stirring mechanism; and,

[0012] A rinsing mechanism is installed on the reactor body.

[0013] In a preferred embodiment of the reaction vessel for producing mycotoxin-degrading enzymes according to this utility model, a feed pipe is inserted into the top outer wall of the reaction vessel body, a support frame is installed on the outer side of the reaction vessel body near the bottom, and a discharge pipe is provided at the bottom of the reaction vessel body.

[0014] In a preferred embodiment of the reaction vessel for producing mycotoxin-degrading enzymes according to this utility model, the aeration and stirring mechanism includes a bushing, a stirring shaft is rotatably inserted into the bushing, and multiple spiral stirring blades are fixed on the outer circumferential wall of the stirring shaft.

[0015] As a preferred embodiment of the reaction vessel for producing mycotoxin degrading enzymes according to this utility model, the stirring shaft has a vertical airflow channel that runs through both the upper and lower ends. The outer walls on both sides of the stirring shaft have conical holes, each conical hole having a small diameter end and a large diameter end. The small diameter end of the conical hole is connected to the airflow channel, and the large diameter end of the conical hole leads to the interior of the reaction vessel body.

[0016] As a preferred embodiment of the reaction vessel for producing mycotoxin-degrading enzymes according to this utility model, the inner wall of the conical hole is coated with a hydrophobic coating of polytetrafluoroethylene, and the outer wall of the stirring shaft near the conical hole is coated with a titanium dioxide photocatalytic coating.

[0017] In a preferred embodiment of the reaction vessel for producing mycotoxin-degrading enzymes according to this utility model, a connecting rod is installed at the bottom end of the stirring shaft, and an arc-shaped scraper is installed at the bottom end of the connecting rod, with the bottom of the arc-shaped scraper adhering to the bottom inner wall of the reaction vessel body.

[0018] As a preferred embodiment of the reaction vessel for producing mycotoxin degrading enzymes according to this utility model, the gas-filling mechanism includes an air pump, which is installed on the surface of the reaction vessel body, located directly above the stirring shaft, and the output end of the air pump passes through the inside of the bushing.

[0019] In a preferred embodiment of the reaction vessel for producing mycotoxin degrading enzymes according to this utility model, the driving mechanism includes a motor, which is mounted on the surface of the reaction vessel body. A transmission shaft is fixed at the bottom end of the motor output shaft, and a transmission assembly is provided on the outer wall of the transmission shaft. The other end of the transmission assembly is mounted on the outer wall of the stirring shaft.

[0020] In a preferred embodiment of the reaction vessel for producing mycotoxin-degrading enzymes according to this utility model, the transmission assembly includes a drive wheel, a conveyor belt, and a transmission wheel. The drive wheel is sleeved on the outer circumference of the transmission shaft, the transmission wheel is sleeved on the outer circumference of the stirring shaft, and the conveyor belt is sleeved on the outer walls of the drive wheel and the transmission wheel.

[0021] In a preferred embodiment of the reaction vessel for producing mycotoxin-degrading enzymes according to this utility model, the rinsing mechanism includes an annular tube installed at the inner edge of the top of the reaction vessel body. A water inlet pipe is inserted into the surface of the annular tube, one end of which is located above the reaction vessel body. Multiple nozzles are installed on the bottom outer wall of the annular tube at equal intervals.

[0022] The beneficial effects of this invention's reaction vessel for producing mycotoxin-degrading enzymes are as follows: Inside the reaction vessel, the microbial fermentation broth is stirred and mixed by a stirring shaft and a spiral stirring paddle. Simultaneously, the stirring shaft has an internal airflow channel and a conical hole on its outer side. After oxygen is introduced through the aeration mechanism, high-pressure gas is input through the shaft. The centrifugal force of rotation breaks the bubbles into micron-sized particles. The dispersed conical holes guide the bubbles to a uniform axial distribution, preventing localized gas accumulation. Precise aeration reduces large bubbles generated by traditional bottom bubbling, lowering the foaming rate of the fermentation broth. The stirring shear force during the rotation of the stirring shaft directly breaks the bubbles, preventing localized oxidative damage to the microbial cells. Attached Figure Description

[0023] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Among them:

[0024] Figure 1 This is a schematic diagram of the overall structure of a reactor used for the production of a fungal toxin-degrading enzyme.

[0025] Figure 2 This is a schematic diagram of the internal structure of a reactor used for the production of a fungal toxin-degrading enzyme.

[0026] Figure 3 for Figure 2 Another structural diagram from another angle.

[0027] Figure 4 This is a partial cross-sectional schematic diagram of the aeration and stirring mechanism of a reactor used for the production of a fungal toxin-degrading enzyme. Detailed Implementation

[0028] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0029] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0030] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that excludes other embodiments.

[0031] Example 1

[0032] Reference Figure 1 and Figure 2 This is the first embodiment of the present invention. This embodiment provides a reaction vessel for the production of fungal toxin degrading enzymes, which can achieve precise aeration and reduce the foaming rate of fermentation liquid. It includes a reaction vessel body 100.

[0033] A ventilation and stirring mechanism 500 is installed at the vertical centerline inside the reactor body 100;

[0034] The gas filling mechanism 300 is installed on the reactor body 100 and is used to supply oxygen to the gas-blowing and stirring mechanism 500.

[0035] A drive mechanism 200 is mounted on the reactor body 100, and one end of the drive mechanism 200 drives the rotation of the aeration and stirring mechanism 500; and,

[0036] The rinsing mechanism 400 is installed on the reactor body 100.

[0037] Specifically, a feed pipe 101 is inserted into the top outer wall of the reactor body 100, a support frame 102 is installed on the outer side of the reactor body 100 near the bottom, and a discharge pipe 103 is provided at the bottom of the reactor body 100.

[0038] In use, microbial fermentation broth is introduced into the reactor body 100 through the feed pipe 101. The microbial fermentation broth is a liquid mixture containing the target product produced by the metabolic activities of microorganisms during their growth and reproduction under specific culture conditions. The target product can be a fungal toxin degrading enzyme. The enzyme is rapidly produced in the reactor body 100 by stirring and aeration. Then, the drive mechanism 200 is started to drive the rotation of the aeration and stirring mechanism 500. At the same time, the aeration mechanism 300 is started to aerate the aeration and stirring mechanism 500. While the aeration and stirring mechanism 500 stirs the fermentation broth, it also introduces gas into the fermentation broth, so that the fermentation broth can rapidly produce enzymes. After completion, the target product is discharged through the discharge pipe 103. Subsequently, the interior of the reactor body 100 can be rinsed by the rinsing mechanism 400 for the next use.

[0039] Example 2

[0040] Reference Figures 1 to 4 This is the second embodiment of the present invention. Unlike the previous embodiment, the aeration and stirring mechanism 500 includes a bushing 505. A stirring shaft 501 is rotatably inserted into the bushing 505. Multiple spiral stirring paddles 502 are fixed on the outer circumference of the stirring shaft 501.

[0041] The drive mechanism 200 drives the stirring shaft 501 and the spiral stirring paddle 502 to rotate, thereby stirring the microbial fermentation broth, making it evenly mixed, and facilitating its fermentation.

[0042] Furthermore, the stirring shaft 501 has a vertical airflow channel 506 that runs through both the top and bottom ends. Both sides of the outer wall of the stirring shaft 501 have conical holes 507. The conical hole 507 includes a small diameter end and a large diameter end. The small diameter end of the conical hole 507 is connected to the airflow channel 506, and the large diameter end of the conical hole 507 leads to the interior of the reactor body 100.

[0043] The aeration mechanism 300 introduces high-pressure gas into the airflow channel 506. The high-pressure gas is input through the shaft and the centrifugal force of rotation breaks the bubbles into micron-sized particles. The bubbles are guided to be evenly distributed along the axial direction through the dispersed conical holes 507, avoiding local gas accumulation. Precise aeration can reduce the large bubbles generated by traditional bottom bubbling and reduce the foaming rate of the fermentation liquid.

[0044] Furthermore, the inner wall of the conical hole 507 is coated with a hydrophobic polytetrafluoroethylene coating, and the outer wall of the stirring shaft 501 near the conical hole 507 is coated with a titanium dioxide photocatalytic coating.

[0045] The inner side of the conical hole 507 is coated with a hydrophobic polytetrafluoroethylene coating to reduce liquid wettability. The titanium dioxide photocatalytic coating around the conical hole 507 helps guide the bubbles to detach quickly, allowing the gas to enter the fermentation broth.

[0046] Furthermore, a connecting rod 503 is installed at the bottom end of the stirring shaft 501, and an arc-shaped scraper 504 is installed at the bottom end of the connecting rod 503. The bottom of the arc-shaped scraper 504 is attached to the bottom inner wall of the reactor body 100.

[0047] During use, the stirring shaft 501 drives the connecting rod 503 and the arc-shaped scraper 504 to move, which facilitates the stirring of the solid matter of fermentation liquid accumulated at the bottom of the reactor body 100, thereby making it easier to mix the fermentation liquid evenly.

[0048] Example 3

[0049] Reference Figures 1 to 3 This is the third embodiment of the present invention. Unlike the previous embodiment, the air filling mechanism 300 includes an air pump 301. The air pump 301 is installed on the surface of the reactor body 100 and is located directly above the stirring shaft 501. The output end of the air pump 301 passes through the inside of the bushing 505.

[0050] After starting the air pump 301, the gas can be sent into the airflow channel 506 inside the stirring shaft 501.

[0051] Furthermore, the drive mechanism 200 includes a motor 201, which is mounted on the surface of the reactor body 100. A transmission shaft 202 is fixed to the bottom end of the output shaft of the motor 201. A transmission assembly is provided on the outer wall of the transmission shaft 202, and the other end of the transmission assembly is mounted on the outer wall of the stirring shaft 501.

[0052] Furthermore, the transmission assembly includes a drive wheel 203, a conveyor belt 204, and a transmission wheel 205. The drive wheel 203 is sleeved on the outer circumference of the transmission shaft 202, the transmission wheel 205 is sleeved on the outer circumference of the stirring shaft 501, and the conveyor belt 204 is sleeved on the outer walls of the drive wheel 203 and the transmission wheel 205.

[0053] The motor 201 is started, which drives the transmission shaft 202 to rotate. The drive wheel 203, the conveyor belt 204 and the transmission wheel 205 drive the stirring shaft 501 to rotate, thereby facilitating the stirring of the fermentation liquid.

[0054] Furthermore, the rinsing mechanism 400 includes an annular tube 402, which is installed at the inner edge of the top of the reactor body 100. A water inlet pipe 401 is inserted into the surface of the annular tube 402, with one end of the water inlet pipe 401 located above the reactor body 100. Multiple nozzles 403 are evenly distributed on the bottom outer wall of the annular tube 402.

[0055] After the fermentation of the microbial fermentation broth is completed, the product is discharged through the discharge pipe 103, and then water is introduced through the water inlet pipe 401, passing through the annular pipe 402 and entering the nozzle 403. The nozzle 403 washes the inner wall of the reactor body 100, making the inside of the reactor body 100 cleaner and facilitating subsequent work.

[0056] It should be understood that numerous specific implementation decisions can be made during the development of any practical implementation, such as in any engineering or design project. Such development efforts may be complex and time-consuming, but for those skilled in the art who benefit from this disclosure, the development effort will be a routine task in design, manufacturing, and production without requiring extensive experimentation.

[0057] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A reaction vessel for producing fungal toxin-degrading enzymes, characterized in that: include, Reactor body (100); A ventilation and stirring mechanism (500) is installed at the vertical centerline inside the reactor body (100); An aeration mechanism (300) is installed on the reactor body (100) and is used to supply oxygen to the aeration and stirring mechanism (500); A drive mechanism (200) is mounted on the reactor body (100), one end of which drives the rotation of the aeration and stirring mechanism (500); and, A rinsing mechanism (400) is mounted on the reactor body (100).

2. The reaction vessel for producing mycotoxin-degrading enzymes as described in claim 1, characterized in that: A feed pipe (101) is inserted into the top outer wall of the reactor body (100), a support frame (102) is installed on the outer side of the reactor body (100) near the bottom, and a discharge pipe (103) is provided at the bottom of the reactor body (100).

3. The reaction vessel for producing mycotoxin-degrading enzymes as described in claim 1 or 2, characterized in that: The aeration and stirring mechanism (500) includes a bushing (505), and a stirring shaft (501) is rotatably inserted inside the bushing (505). Multiple spiral stirring blades (502) are fixed on the outer circumferential wall of the stirring shaft (501).

4. The reaction vessel for producing mycotoxin-degrading enzymes as described in claim 3, characterized in that: The stirring shaft (501) has a vertical airflow channel (506) that runs through both the top and bottom. Both sides of the outer wall of the stirring shaft (501) have conical holes (507). The conical hole (507) includes a small diameter end and a large diameter end. The small diameter end of the conical hole (507) is connected to the airflow channel (506), and the large diameter end of the conical hole (507) leads to the interior of the reactor body (100).

5. The reaction vessel for producing mycotoxin-degrading enzymes as described in claim 4, characterized in that: The inner wall of the conical hole (507) is coated with a hydrophobic polytetrafluoroethylene coating, and the outer wall of the stirring shaft (501) near the conical hole (507) is coated with a titanium dioxide photocatalytic coating.

6. The reaction vessel for producing mycotoxin-degrading enzymes as described in claim 5, characterized in that: A connecting rod (503) is installed at the bottom end of the stirring shaft (501), and an arc-shaped scraper (504) is installed at the bottom end of the connecting rod (503). The bottom of the arc-shaped scraper (504) is attached to the bottom inner wall of the reactor body (100).

7. The reaction vessel for producing mycotoxin-degrading enzymes as described in claim 6, characterized in that: The aeration mechanism (300) includes an air pump (301), which is mounted on the surface of the reactor body (100). The air pump (301) is located directly above the stirring shaft (501), and the output end of the air pump (301) passes through the inside of the bushing (505).

8. The reaction vessel for producing mycotoxin-degrading enzymes as described in claim 7, characterized in that: The drive mechanism (200) includes a motor (201), which is mounted on the surface of the reactor body (100). A transmission shaft (202) is fixed at the bottom of the output shaft of the motor (201). A transmission assembly is provided on the outer wall of the transmission shaft (202), and the other end of the transmission assembly is mounted on the outer wall of the stirring shaft (501).

9. The reaction vessel for producing mycotoxin-degrading enzymes as described in claim 8, characterized in that: The transmission assembly includes a drive wheel (203), a conveyor belt (204), and a drive wheel (205). The drive wheel (203) is sleeved on the outer circumference of the drive shaft (202), the drive wheel (205) is sleeved on the outer circumference of the stirring shaft (501), and the conveyor belt (204) is sleeved on the outer walls of the drive wheel (203) and the drive wheel (205).

10. The reaction vessel for producing mycotoxin-degrading enzymes as described in claim 9, characterized in that: The rinsing mechanism (400) includes an annular tube (402), which is installed at the inner edge of the top of the reactor body (100). A water inlet pipe (401) is inserted into the surface of the annular tube (402), with one end of the water inlet pipe (401) located above the reactor body (100). Multiple nozzles (403) are installed on the bottom outer wall of the annular tube (402) at equal intervals.

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