Card type glass inoculation bottle convenient to observe

Abstract

The utility model discloses a clamping type glass inoculation bottle convenient to observe. The clamping type glass inoculation bottle comprises a bottle body and a bottle cap which are matched with each other for use, an inoculation liquid feeding opening for feeding materials into the bottle body is formed in the bottle opening, and a plurality of convex lenses are embedded in the middle of the bottle body; the bottle cap comprises a cap body and a cap top fixed to the top face of the cap body, a sampling opening is formed in the cap top, and a sampling puncture silica gel plug is embedded in the sampling opening. The shortest distance from the axis of the inoculation liquid feeding port to the edge of the bottle opening is equal to the shortest distance from the axis of the sampling port to the edge of the cap top, and the axes of the inoculation liquid feeding port and the sampling port do not coincide with the axis of the bottle body or the bottle cap. By means of the design that the convex lenses are symmetrically welded to the two sides, visual distortion during single-side observation is effectively eliminated, dead-angle-free amplification observation is provided, the curvature radius of each convex lens is 5-15 mm, 2-4 times of optical amplification can be achieved, and microbial colonies of 50-200 microns can be clearly observed without external equipment.

Description

Technical Field

[0001] This utility model relates to the field of experimental equipment technology, specifically to a cartridge-type glass inoculation bottle that is easy to observe. Background Technology

[0002] In the fields of biotechnology such as microbial culture, cell culture and vaccine preparation, inoculation bottles are one of the basic pieces of equipment commonly used in laboratories. Traditional glass inoculation bottles are usually cylindrical or conical in design, which has problems such as inconvenient observation and cumbersome sampling operations, making it difficult to meet the needs of modern biological experiments for efficient and precise operation.

[0003] Currently, the inoculation bottles on the market mainly suffer from the following technical defects:

[0004] (1) Observation limitations: Due to the refraction effect of the curved surface, ordinary glass bottles are prone to visual distortion when observing the growth of microorganisms or cells inside the bottle, which affects the accurate judgment of the culture state. In particular, the observation of tiny colonies or cell clusters often requires the use of external magnification equipment.

[0005] (2) Inconvenient sampling: Conventional designs require the bottle cap to be fully opened during sampling, which is not only cumbersome but also increases the risk of culture contamination. Although some products use rubber stopper puncture sampling, most of them have problems such as decreased sealing after puncture and loss of elasticity after repeated use.

[0006] Therefore, there is an urgent need to develop a new type of inoculation bottle that integrates high-definition observation, convenient sampling, and reliable sealing to meet the increasingly demanding technical requirements of modern biological experiments for culture containers. Utility Model Content

[0007] The technical problem to be solved by this invention is to provide a convenient and easy-to-observe cartridge glass inoculation bottle, addressing the shortcomings of existing technologies.

[0008] The technical problem to be solved by this utility model is achieved through the following technical solution: a cartridge glass inoculation bottle that is easy to observe, which includes a bottle body and a bottle cap that are used in conjunction with each other.

[0009] The bottle body has a sealed bottom and bottle mouth. An inoculation port for adding the inoculation solution into the bottle is opened at the bottle mouth. Several convex lenses are embedded in the middle of the bottle body to facilitate observation of the inoculation status of the inoculation solution in the bottle.

[0010] The bottle cap includes a cap body and a cap top fixed to the top surface of the cap body. The inner circumferential surface of the cap body is threadedly connected to the outer circumferential surface of the bottle mouth. A sampling port is provided on the cap top, which can be moved to the top of the inoculation solution feeding port during the screwing of the cap. A sampling puncture silicone plug is embedded in the sampling port.

[0011] The shortest distance from the axis of the inoculation solution feeding port to the edge of the bottle mouth is equal to the shortest distance from the axis of the sampling port to the edge of the cap. The axes of the inoculation solution feeding port and the sampling port do not coincide with the axis of the bottle body or the cap.

[0012] The technical problem to be solved by this utility model can also be achieved through the following technical solution: the above-mentioned easy-to-observe cartridge glass inoculation bottle has a gripping concave surface formed at the bottle mouth to facilitate holding the bottle.

[0013] The technical problem to be solved by this utility model can also be achieved through the following technical solution: the above-mentioned easy-to-observe cartridge glass inoculation bottle has millimeter scale lines with length units of millimeters vertically sprayed on the outer peripheral surface of the bottle body.

[0014] The technical problem to be solved by this utility model can also be achieved through the following technical solution: the above-mentioned easy-to-observe card-type glass inoculation bottle, wherein a pair of convex lenses are provided, and the pair of convex lenses are symmetrically embedded on both sides of the bottle body by means of fusion welding.

[0015] The technical problem to be solved by this utility model can also be achieved through the following technical solution: the above-mentioned easy-to-observe cartridge glass inoculation bottle has an O-ring fixed between the top of the cap and the body of the cap.

[0016] Compared with the prior art, the beneficial technical effects of this utility model are:

[0017] (1) By using a double-sided symmetrical fused convex lens design, visual distortion during single-sided observation is effectively eliminated, providing magnified observation without blind spots. The radius of curvature of the convex lens is 5-15mm, which can achieve 2-4 times optical magnification. Microbial colonies of 50-200μm can be clearly observed without external equipment.

[0018] (2) The inoculation solution feeding port and sampling port are linked by an eccentric setting. The feeding, culture and sampling modes can be switched by simply rotating the bottle cap. It is highly operable and has good sealing performance. The three-layer sealing system: threaded connection + O-ring seal + puncture silicone plug, so that the air tightness meets the ISO 8362-1 standard. The sampling puncture silicone plug can withstand ≥50 punctures and still maintain a seal. The puncture force retention rate is >90%, which meets the ASTM D412 standard for elastomer tensile properties in the industry, that is, after high-pressure sterilization at 121℃, the elastic modulus change rate is still <5%.

[0019] (3) The concave surface of the bottle mouth is ergonomically designed to provide a stable torque support point, reducing the opening torque by 35%. The external millimeter-level scale line supports direct volume measurement and avoids secondary transfer contamination. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the full cross-sectional structure of the bottle body and cap of this utility model;

[0021] Figure 2 This is a top view of the bottle cap structure of this utility model;

[0022] Figure 3 This is a top view of the bottle body of this utility model.

[0023] Figure label:

[0024] 1. Bottle body; 2. Inoculation solution inlet; 3. Convex lens; 4. Grip concave surface; 5. Cap body; 6. Cap top; 7. O-ring seal; 8. Puncture silicone stopper. Detailed Implementation

[0025] The specific technical solutions of this utility model are further described below with reference to the accompanying drawings, so as to enable those skilled in the art to further understand this utility model, without constituting a limitation on its rights.

[0026] Examples 1-3: A cartridge-type glass inoculation bottle for easy observation, comprising a bottle body 1 and a bottle cap that cooperate with each other, the material of which can be borosilicate glass;

[0027] The bottle body 1 has a sealed bottom and bottle mouth. An inoculation solution inlet 2 is provided at the bottle mouth for adding inoculation solution into the bottle body 1. The inoculation solution inlet 2 can be a round hole. Inoculation solution can be injected into the bottle body 1 through the inoculation solution inlet 2. Several convex lenses 3 are embedded in the middle of the bottle body 1 to facilitate observation of the inoculation status of the inoculation solution in the bottle body 1. The size and specifications of the convex lenses 3 can be selected according to the usage requirements. The colonies in the inoculation bottle can be observed through the convex lenses 3. The convex lenses 3 are provided in pairs. The pair of convex lenses 3 are symmetrically embedded on both sides of the bottle body 1 by welding. The use of a pair of convex lenses 3 can eliminate the visual distortion when observing from one side and provide the effect of magnified observation without blind spots.

[0028] In order to make the bottle body 1 easier to hold, a gripping concave surface 4 is formed at the mouth of the bottle body 1 to facilitate gripping the bottle body 1. It is formed in a roughly arc-shaped structure.

[0029] The bottle cap includes a cap body 5 and a cap top 6 fixed to the top surface of the cap body 5. The inner circumferential surface of the cap body 5 is threadedly connected to the outer circumferential surface of the bottle mouth of the bottle body 1. A sampling port is provided on the cap top 6, which can be moved to the top of the inoculation solution feeding port 2 during the tightening of the cap. The sampling port is formed as a roughly circular hole, and a sampling puncture silicone plug 8 is embedded in the sampling port. It should be noted that when the cap is completely tightened on the bottle body 1, the sampling port and the inoculation solution feeding port 2 are misaligned. When the cap is rotated counterclockwise by a certain angle, such as 90°, the axis of the sampling port and the inoculation solution feeding port 2 can be aligned, and sampling can then begin.

[0030] The shortest distance from the axis of the inoculation solution feeding port 2 to the edge of the bottle mouth is equal to the shortest distance from the axis of the sampling port to the edge of the cap 6. The specific distance value can be selected according to the usage requirements. The axes of the inoculation solution feeding port 2 and the sampling port do not coincide with the axis of the bottle body 1 or the bottle cap. In addition, in order to facilitate the observation of the liquid level in the inoculation bottle, the outer circumferential surface of the bottle body 1 is vertically sprayed with millimeter scale lines with a length unit of millimeters (not shown in the figure).

[0031] To improve the sealing effect between the bottle body 1 and the bottle cap, an O-ring 7 is fixed between the cap top 6 and the cap body 5.

[0032] The process of using the easy-to-observe cartridge glass inoculation bottle in Example 1 is as follows:

[0033] 1. Preparation before use: Place the assembled inoculation bottle, including bottle body 1, bottle cap and silicone stopper, in an autoclave and sterilize at 121℃ for 20-30 minutes. After cooling to room temperature, place it on a clean bench for later use.

[0034] 2. Adding procedure: Use a pipette to inject the culture medium through the filling port. Control the liquid level by the 1 mm mark on the bottle body. It is recommended to fill the bottle to 30-70% of the total volume of the bottle body to avoid the influence of the lens area on the liquid surface. After adding the medium, screw the cap on the bottle body at the mouth.

[0035] 3. Inoculation and culture: Use a puncture needle to puncture the silicone stopper and transfer the microbial sample into the inoculation bottle. The inoculation distribution can be observed in real time through the convex lens 3.

[0036] 4. Sampling procedure: Open the sampling channel and rotate the bottle cap 90° counterclockwise so that the sampling port coincides with the axis of the inoculation solution feeding port 2. Sampling can then begin. When sampling, wipe the top 6 area of ​​the bottle cap with 75% alcohol. Use a sterile syringe (1-5mL recommended) to puncture the silicone stopper 8 vertically and slowly extract the required amount of sample.

Claims

1. A cartridge-type glass inoculation bottle for easy observation, characterized in that: It includes the bottle body and cap that work together; The bottle body has a sealed bottom and bottle mouth. An inoculation port for adding the inoculation solution into the bottle is opened at the bottle mouth. Several convex lenses are embedded in the middle of the bottle body to facilitate observation of the inoculation status of the inoculation solution in the bottle. The bottle cap includes a cap body and a cap top fixed to the top surface of the cap body. The inner circumferential surface of the cap body is threadedly connected to the outer circumferential surface of the bottle mouth. A sampling port is provided on the cap top, which can be moved to the top of the inoculation solution feeding port during the screwing of the cap. A sampling puncture silicone plug is embedded in the sampling port. The shortest distance from the axis of the inoculation solution feeding port to the edge of the bottle mouth is equal to the shortest distance from the axis of the sampling port to the edge of the cap. The axes of the inoculation solution feeding port and the sampling port do not coincide with the axis of the bottle body or the cap.

2. The easy-to-observe cartridge glass inoculation bottle according to claim 1, characterized in that: The bottle body has a gripping concave surface at the bottle mouth to facilitate holding the bottle body.

3. The easy-to-observe cartridge glass inoculation bottle according to claim 1, characterized in that: The outer circumferential surface of the bottle is vertically sprayed with millimeter-scale lines with a length unit of millimeters.

4. The easy-to-observe cartridge glass inoculation bottle according to claim 1, characterized in that: The convex lens is provided in a pair, and the pair of convex lenses are symmetrically embedded on both sides of the bottle body by means of welding.

5. The easy-to-observe cartridge glass inoculation bottle according to claim 1, characterized in that: The radius of curvature of a convex lens is 5mm-15mm.

6. The easy-to-observe cartridge glass inoculation bottle according to claim 1, characterized in that: An O-ring is fixed between the top of the cover and the body of the cover.

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