Device for filling magnetic bead liquid

Abstract

The utility model belongs to the technical field of biomedical engineering, and particularly relates to a magnetic bead liquid filling device which comprises a base, and a stirring mechanism, a filling mechanism and a moving mechanism are arranged on the base. The stirring mechanism comprises a stirring barrel, a motor is installed in the middle of the top end of the stirring barrel, an output shaft of the motor penetrates through the top wall of the stirring barrel and is sequentially provided with an upper stirring assembly, a reciprocating assembly and a lower stirring assembly, a liquid inlet pipe is arranged at the top end of the stirring barrel, and a liquid outlet is formed in the bottom end of the stirring barrel; the filling mechanism comprises a downward pressing assembly, a liquid injection assembly is arranged at the execution end of the downward pressing assembly and communicates with the liquid outlet end of a peristaltic pump, the liquid inlet end of the peristaltic pump communicates with the liquid outlet, a hole plate is arranged on the moving mechanism in a limiting mode, and the liquid injection assembly and the hole plate are correspondingly arranged. The magnetic bead liquid mixing device is simple in structure, small in size, low in manufacturing cost and capable of improving the mixing uniformity of magnetic bead liquid.

Description

Technical Field

[0001] This utility model belongs to the field of biomedical engineering technology, and in particular relates to a device for filling magnetic bead liquid. Background Technology

[0002] Nucleic acid extraction is an important step in molecular biology experiments. It can isolate pure DNA or RNA from various biological samples for subsequent detection or analysis. There are many methods for nucleic acid extraction, one of the most commonly used being the magnetic bead method.

[0003] Magnetic bead-based nucleic acid extraction reagents utilize magnetic beads to achieve nucleic acid separation and purification. Magnetic beads are nanoscale magnetic particles with functional groups labeled on their surface, capable of specifically adsorbing onto nucleic acids under specific conditions. The aggregation and dispersion of the magnetic beads are controlled by an external magnetic field. The magnetic bead method mainly achieves rapid and efficient extraction and purification of nucleic acids through four steps: lysis, adsorption, washing, and elution. It has advantages such as high throughput, simple operation, safety and non-toxicity, and high extraction purity.

[0004] Currently, when filling magnetic bead solutions, the magnetic beads settle quickly, requiring frequent manual shaking during the filling process. This not only increases the workload but also greatly reduces work efficiency. Although some automated filling equipment has appeared on the market, it occupies a large area, is expensive, and is not easy to move.

[0005] Therefore, it is necessary to design a device for filling magnetic beads to solve the above-mentioned technical problems. Utility Model Content

[0006] The purpose of this invention is to provide a device for filling magnetic bead liquid to solve the problems existing in the prior art.

[0007] To achieve the above objectives, this utility model provides a device for filling magnetic bead liquid, including a base, on which a stirring mechanism, a filling mechanism, and a moving mechanism are arranged; the stirring mechanism includes a stirring tank, a motor is installed at the top center of the stirring tank, the output shaft of the motor passes through the top wall of the stirring tank and is sequentially arranged with an upper stirring component, a reciprocating component, and a lower stirring component, the top of the stirring tank is provided with a liquid inlet pipe, and the bottom of the stirring tank is provided with a liquid outlet; the filling mechanism includes a pressing component, the execution end of the pressing component is provided with a liquid injection component, the liquid injection component is connected to the liquid outlet of a peristaltic pump, the liquid inlet of the peristaltic pump is connected to the liquid outlet, the upper limit of the moving mechanism is provided with an orifice plate, and the liquid injection component is correspondingly arranged with the orifice plate.

[0008] Preferably, the upper stirring assembly includes a rotating rod two connected to the output shaft of the motor, and a plurality of stirring blades are circumferentially connected to the outer wall of the rotating rod two. A scraper is connected to the end of the stirring blades away from the rotating rod two, and the scraper is in frictional contact with the inner wall of the stirring tank.

[0009] Preferably, the reciprocating assembly includes a reciprocating threaded rod connected to the bottom end of the rotating rod, a convection plate coaxially threaded onto the reciprocating threaded rod, the convection plate being slidably limited on the inner wall of the mixing tank, and the convection plate having multiple through holes, the cross-section of which is trapezoidal.

[0010] Preferably, the lower stirring assembly includes a rotating rod connected to the bottom end of the reciprocating threaded rod, and a spiral blade is wound around the rotating rod.

[0011] Preferably, the pressing component includes a slide rod one and a slide rod two vertically fixedly connected to the top of the base. A slide platform is slidably sleeved between the slide rod two and the slide rod one. The liquid injection component is disposed on the side of the slide platform near the moving mechanism. The lower part of the operating lever is hinged to the end of the slide platform near the liquid injection component. A hinge seat is connected to the base. One end of an arc-shaped connecting plate is hinged to the hinge seat. The other end of the arc-shaped connecting plate is hinged to the bottom end of the operating lever. An adjusting block is disposed at the lower part of the slide rod two. A spring one is slidably sleeved on the slide rod two. The top end of the spring one abuts against the bottom end of the slide platform, and the other end of the spring one abuts against the top end of the adjusting block.

[0012] Preferably, the injection assembly includes a pressure plate connected to the bottom of the slide table, and a plurality of liquid storage tubes are connected at equal intervals to the bottom of the pressure plate. The liquid storage tubes are connected to the outlet of the peristaltic pump through a pipeline. A flow valve is provided on the pipeline. A plurality of injection needle valves are provided at equal intervals to the bottom of the liquid storage tubes. The injection needle valves are provided corresponding to the holes on the orifice plate.

[0013] Preferably, the injection needle valve includes an injection tube connected to the bottom end of the reservoir tube, a pin slidably disposed inside the injection tube, the bottom end of the pin penetrating the bottom wall of the injection tube and connected to a small ball, a valve core fixedly sleeved on the pin, the valve core having a conical cross-section, a conical groove adapted to the valve core being opened on the inner bottom wall of the injection tube, a plurality of injection holes being opened laterally on the groove wall of the conical groove, a bracket slidably sleeved on the upper part of the pin, the bracket being fixedly connected to the inner wall of the injection tube, a second spring slidably sleeved on the pin, the top end of the second spring abutting against the bracket, and the bottom end of the second spring abutting against the top end of the valve core.

[0014] Preferably, the moving mechanism includes a sliding plate slidably disposed on the base, a positioning frame connected to the top of the sliding plate, and the perforated plate being positioned on the sliding plate through the positioning frame.

[0015] Compared with the prior art, the present invention has the following advantages and technical effects:

[0016] This invention provides a device for filling magnetic bead liquid. A motor 8 drives an upper and lower stirring assembly to stir the magnetic bead liquid in a stirring tank 5. Simultaneously, a reciprocating assembly moves up and down along the inner wall of the stirring tank 5, changing the flow rate of the magnetic bead liquid and facilitating thorough mixing. A moving mechanism positions the orifice plate 16 and pushes it directly below the injection assembly. A peristaltic pump 2 pumps the stirred magnetic bead liquid onto the injection assembly. A pressing assembly then pushes the injection assembly against the orifice plate 16, injecting the magnetic bead liquid from the injection assembly into the holes of the orifice plate 16.

[0017] This invention has a simple structure, small size, and low cost, while also improving the uniformity of magnetic bead liquid mixing. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the structure of a device for filling magnetic bead liquid according to the present invention;

[0020] Figure 2 This is a schematic diagram of the stirring mechanism in this utility model;

[0021] Figure 3 This is a cross-sectional view of the needle valve in this utility model;

[0022] Figure 4 This is a top view of the moving mechanism in this utility model;

[0023] The components are as follows: 1. Base; 2. Peristaltic pump; 3. Adjusting block; 4. Arc-shaped connecting plate; 5. Stirring tank; 6. Slide rod one; 7. Inlet pipe; 8. Motor; 9. Slide table; 10. Control lever; 11. Slide rod two; 12. Pressure plate; 13. Storage pipe; 14. Injection pipe; 15. Spring one; 16. Orifice plate; 17. Slide plate; 18. Spiral blade; 19. Rotating rod one; 20. Convection plate; 21. Through hole; 22. Stirring blade; 23. Scraper; 24. Rotating rod two; 25. Reciprocating threaded rod; 26. Slider; 27. Guide groove; 28. Outlet; 29. ​​Support; 30. Spring two; 31. Valve core; 32. Injection hole; 33. Pin; 34. Small ball; 35. Pulley; 36. Slide groove; 37. Positioning frame; 38. Hinge seat. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0025] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0026] The following explanations are provided for the technical terms used in the instruction manual:

[0027] The core components of a peristaltic pump include a pump head, a hose, and a drive. The pump head contains rollers or sliders, and the drive rotates or slides these components, periodically squeezing the hose. When the hose returns to its original shape after squeezing, a negative pressure is created, drawing in fluid; when squeezed again, the fluid is propelled to the outlet. Throughout the process, the fluid only comes into contact with the inner wall of the hose, preventing contamination and leakage.

[0028] Magnetic bead liquid is a suspension formed by dispersing magnetic microparticles (such as nano-iron oxide) in a specific liquid medium. It combines the magnetic response characteristics of magnetic materials with the fluidity of liquids, and is widely used in biomedicine, analytical testing, materials science, and other fields. The following is a detailed introduction to magnetic bead liquid:

[0029] I. Composition and Properties of Magnetic Bead Liquid

[0030] Magnetic particles:

[0031] The core component of magnetic bead liquid is nano- or micro-sized magnetic particles, such as nano-iron oxide (Fe3O4). These particles are superparamagnetic, meaning they can be rapidly magnetized under the influence of an external magnetic field, and the magnetism disappears after the magnetic field is removed, leaving no residual magnetism.

[0032] Dispersion medium:

[0033] Magnetic microparticles are typically dispersed in water, organic solvents, or biocompatible liquids to form stable suspensions. The choice of dispersion medium depends on the application requirements; for example, water or buffer solutions are often used in the biomedical field to ensure the stability of bioactive molecules.

[0034] Surface finishing:

[0035] To prevent the aggregation of magnetic particles and enhance their functionality, their surfaces are often chemically modified, such as by carboxylation, amination, or coating with biomolecules (e.g., antibodies, nucleic acid aptamers). These modifications enable the magnetic beads to specifically bind to target molecules, achieving separation, enrichment, or detection.

[0036] II. Application areas of magnetic bead liquid

[0037] Biomedicine:

[0038] Nucleic acid extraction: After the magnetic beads bind to the nucleic acid, they are rapidly separated by an external magnetic field, achieving high-throughput and automated nucleic acid extraction.

[0039] Protein purification: Using antibody- or ligand-modified magnetic beads to specifically capture target proteins, simplifying the purification process.

[0040] Cell separation: Rapid separation and enrichment of cells are achieved through magnetic beads labeled with cell-specific antigens on their surface.

[0041] Analysis and testing:

[0042] Immunoassay: Magnetic beads serve as a solid-phase carrier, binding antigens or antibodies for chemiluminescence detection, etc.

[0043] Biosensors: Combining magnetic beads with bio-recognition elements to construct highly sensitive biosensors for detecting biomolecules or environmental pollutants.

[0044] Materials Science:

[0045] Magnetic composite materials: Magnetic beads are used as functional fillers to prepare magnetic composite materials for catalysis, adsorption or drug delivery.

[0046] Nanofluids: Magnetic beads serve as the mobile phase in microfluidic systems to enable sample manipulation and separation.

[0047] III. Advantages of Magnetic Bead Liquid

[0048] Highly efficient separation: Under the action of an external magnetic field, the magnetic bead liquid can quickly separate the target molecules, shortening the operation time.

[0049] Easy to automate: Magnetic bead liquid is compatible with automated equipment and is suitable for high-throughput, standardized bioanalytical processes.

[0050] Biocompatibility: Surface-modified magnetic beads can maintain the stability of bioactive molecules, making them suitable for biomedical applications.

[0051] Multifunctionality: The performance of magnetic bead liquid can be customized by adjusting the size, surface properties and dispersion medium of the magnetic particles.

[0052] IV. Preparation and Precautions of Magnetic Bead Solution

[0053] Preparation method

[0054] The preparation of magnetic bead solutions typically involves the synthesis, surface modification, and dispersion of magnetic microparticles. It is essential to ensure uniform dispersion of the magnetic microparticles, avoid aggregation, and maintain their magnetic responsiveness and biological activity.

[0055] Precautions

[0056] Stability: The magnetic bead liquid needs to be stored under specific conditions to avoid sedimentation or aggregation of magnetic particles.

[0057] Safety: Magnetic microparticles may be biotoxic, and the safety of their surface modification and dispersion media must be ensured.

[0058] Compatibility: Select magnetic bead solutions that are compatible with the target molecules and detection methods to avoid non-specific binding or interference.

[0059] Reference Figures 1 to 4 As shown, this utility model provides a device for filling magnetic bead liquid, including a base 1, on which a stirring mechanism, a filling mechanism and a moving mechanism are provided; the stirring mechanism includes a stirring tank 5, a motor 8 is installed at the top center of the stirring tank 5, the output shaft of the motor 8 passes through the top wall of the stirring tank 5 and is sequentially provided with an upper stirring component, a reciprocating component and a lower stirring component, an inlet pipe 7 is provided at the top of the stirring tank 5 and an outlet 28 is provided at the bottom of the stirring tank 5; the filling mechanism includes a pressing component, an injection component is provided at the execution end of the pressing component, the injection component is connected to the outlet end of a peristaltic pump 2, the inlet end of the peristaltic pump 2 is connected to the outlet 28, and an orifice plate 16 is provided at the upper limit of the moving mechanism, and the injection component is correspondingly provided with the orifice plate 16.

[0060] The upper and lower stirring components, driven by the motor 8, stir the magnetic bead liquid in the stirring tank 5. Simultaneously, a reciprocating component moves up and down along the inner wall of the stirring tank 5, changing the flow rate of the magnetic bead liquid and promoting thorough mixing. A moving mechanism positions the orifice plate 16 and pushes it directly below the injection component. A peristaltic pump 2 pumps the stirred magnetic bead liquid onto the injection component. A pressing component then pushes the injection component against the orifice plate 16, injecting the magnetic bead liquid from the injection component into the holes of the orifice plate 16.

[0061] This invention has a simple structure, small size, and low cost, while also improving the uniformity of magnetic bead liquid mixing.

[0062] Furthermore, the upper stirring assembly includes a rotating rod 24 connected to the output shaft of the motor 8. Multiple stirring blades 22 are circumferentially connected to the outer wall of the rotating rod 24. A scraper 23 is connected to the end of the stirring blade 22 away from the rotating rod 24. The scraper 23 is in frictional contact with the inner wall of the stirring tank 5.

[0063] The motor 8 drives the rotating rod 24 to rotate, and the rotating rod 24 drives the stirring blade 22 to rotate, thereby stirring the upper magnetic bead liquid in the upper stirring tank 5. The set scraper 23 can remove the magnetic bead liquid on the inner wall of the stirring tank 5.

[0064] Furthermore, the reciprocating assembly includes a reciprocating threaded rod 25 connected to the bottom end of the rotating rod 24. A convection plate 20 is coaxially threaded onto the reciprocating threaded rod 25. The convection plate 20 is slidably limited on the inner wall of the mixing tank 5. Multiple through holes 21 are provided on the convection plate 20, and the cross-section of the through holes 21 is trapezoidal.

[0065] In this embodiment, two guide grooves 27 are formed on the inner wall of the mixing tank 5 in the vertical direction, and two sliders 26 are connected to the edge of the convection plate 20. The sliders 26 are slidably limited in the guide grooves 27.

[0066] The motor 8 drives the rotating rod 24 and the reciprocating threaded rod 25 to rotate. The convection plate 20 moves up and down along the inside of the mixing tank 5 under the rotation of the reciprocating threaded rod 25. The flow rate of the magnetic bead liquid can be changed through the through hole 21 on the convection plate 20, and the magnetic bead liquid stirred by the upper stirring component can enter the lower stirring component for further stirring, thereby improving the uniformity of the stirring of the magnetic bead liquid.

[0067] Furthermore, the lower stirring assembly includes a rotating rod 19 connected to the bottom end of the reciprocating threaded rod 25, with a spiral blade 18 wound around the rotating rod 19.

[0068] The motor 8 drives the second rotating rod 24, the reciprocating threaded rod 25 and the first rotating rod 19 to rotate synchronously. The rotating rod 1 drives the spiral blade 18 to rotate, thereby realizing the secondary stirring of the magnetic bead liquid.

[0069] Furthermore, the pressing component includes a slide rod 6 and a slide rod 11 vertically fixed to the top of the base 1. A slide table 9 is slidably sleeved between the slide rod 11 and the slide rod 6. The liquid injection component is located on the side of the slide table 9 near the moving mechanism. The lower part of the control lever 10 is hinged to the end of the slide table 9 near the liquid injection component. A hinge seat 38 is connected to the base 1. One end of an arc-shaped connecting plate 4 is hinged to the hinge seat 38. The other end of the arc-shaped connecting plate 4 is hinged to the bottom end of the control lever 10. An adjusting block 3 is provided at the lower part of the slide rod 11. A spring 15 is slidably sleeved on the slide rod 11. The top end of the spring 15 abuts against the bottom end of the slide table 9, and the other end of the spring 15 abuts against the top end of the adjusting block 3.

[0070] In this embodiment, the adjusting block 3 is slidably sleeved on the slide rod 11. The adjusting block 3 is threaded with a locking bolt, which can fix the adjusting block 3 at a certain position on the slide rod 11 to adjust the compression of the spring 15. By pressing down the operating lever 10, the operating lever 10 rotates on the slide table 9. At the same time, the bottom end of the operating lever 10 drives the arc-shaped connecting plate 4 to rotate on the hinge seat 38, and forces the slide table 9 to overcome the elastic force of the spring 15 and move smoothly downward along the slide rod 11, thereby driving the liquid injection assembly to press against the orifice plate 16.

[0071] Furthermore, the injection assembly includes a pressure plate 12 connected to the bottom of the slide table 9. Multiple liquid storage tubes 13 are connected at equal intervals to the bottom of the pressure plate 12. The liquid storage tubes 13 are connected to the outlet of the peristaltic pump 2 through pipelines. A flow valve is installed on the pipelines. Multiple injection needle valves are installed at equal intervals to the bottom of the liquid storage tubes 13. The injection needle valves are corresponding to the holes on the orifice plate 16.

[0072] In this embodiment, a total of 96 holes are provided on the orifice plate 16. The bottom end of the pressure plate 12 is connected to 8 liquid storage tubes 13. Each liquid storage tube 13 is threaded with 12 injection needle valves. Each injection needle valve is set to correspond to each hole on the orifice plate 16. The injection needle valve contacts the bottom wall of the hole and triggers the injection needle valve to open, so that the magnetic bead liquid in the liquid storage tube 13 is injected into the hole on the orifice plate 16.

[0073] According to actual usage needs, the injection valve needles corresponding to the holes that do not need to be injected with magnetic beads can be removed and sealed with thread plugs. Alternatively, the flow valves on the corresponding pipelines of each liquid storage tube 13 can be closed. For example, if it is necessary to inject magnetic beads into the second and eighth rows of holes on the orifice plate 16, it is only necessary to close the flow valves of other pipelines.

[0074] Furthermore, the injection needle valve includes an injection tube 14 connected to the bottom end of the reservoir tube 13. A needle 33 is slidably disposed inside the injection tube 14. The bottom end of the needle 33 penetrates the bottom wall of the injection tube 14 and is connected to a small ball 34. A valve core 31 is fixedly sleeved on the needle 33. The cross-section of the valve core 31 is conical. A conical groove adapted to the valve core 31 is opened on the inner bottom wall of the injection tube 14. Multiple injection holes 32 are opened laterally on the groove wall of the conical groove. A bracket 29 is slidably sleeved on the upper part of the needle 33. The bracket 29 is fixedly connected to the inner wall of the injection tube 14. A second spring 30 is slidably sleeved on the needle 33. The top end of the second spring 30 abuts against the bracket 29, and the bottom end of the second spring 30 abuts against the top end of the valve core 31.

[0075] The small ball 34 facilitates the smooth entry of the ejector pin 33 into the hole on the orifice plate 16. When the pressure plate 12 is pressed down further, the small ball 34 of the ejector pin 33 contacts the bottom of the hole on the orifice plate 16, and the valve core 31 moves upward against the elastic force of the second spring 30. The valve core 31 separates from the conical groove and loses its sealing function. The magnetic bead liquid is injected into the hole on the orifice plate 16 through the injection hole 32. It should be noted that the bracket 29 is small enough to ensure that the magnetic bead liquid in the injection tube 14 flows smoothly to the injection hole 32. After the injection is completed, the operating lever 10 is slowly released, and the slide table 9 is reset under the reaction force of the first spring 15. The reset slide table 9 drives the injection needle valve to separate from the orifice plate 16.

[0076] Furthermore, the moving mechanism includes a slide plate 17 that is slidably mounted on the base 1, with a positioning frame 37 connected to the top of the slide plate 17, and the perforated plate 16 is positioned on the slide plate 17 through the positioning frame 37.

[0077] In this embodiment, a notch is provided on one side of the base 1, which allows the slide plate 17 to be inserted. A groove 36 is provided on the opposite side of the notch. Rollers 35 are provided on both sides of the slide plate 17. The rollers 35 are slidably disposed in the groove 36 to realize the pulling of the slide plate 17, which facilitates the handling of the orifice plate 16. The positioning frame 37 is provided to position the orifice plate 16, thereby ensuring that the holes on the orifice plate 16 are aligned with the injection needle valve.

[0078] The device for filling magnetic bead liquid provided by this utility model works as follows: During use, magnetic bead liquid is injected into the stirring tank 5 through the inlet pipe 7. The motor 8 is started for thorough stirring. Then, the peristaltic pump 2 is turned on. The outlet end of the peristaltic pump 2 is connected to the main pipe and is connected to the corresponding storage pipe 13 through 8 branch pipes. The flow rate is set through the flow valve on the branch pipe. The orifice plate 16 to be injected with magnetic bead liquid is placed in the positioning frame 37. Then, the sliding plate 17 is pushed to its maximum position, at which point the holes on the orifice plate 16 are aligned with the injection needle valve. Then, the operating lever 1 is pressed down. 0. The control lever 10 drives the slide 9 to press down the ejector pin 33. The ejector pin 33 drives the valve core 31 to move upward against the elastic force of the second spring 30. The valve core 31 separates from the conical groove and loses its sealing function. The magnetic bead liquid is injected into the hole on the orifice plate 16 through the injection hole 32. After the injection is completed, the control lever 10 is slowly released. The slide 9 is reset under the reaction force of the first spring 15. The reset slide 9 drives the injection needle valve to separate from the orifice plate 16. Then, the slide plate 17 is pulled outward to make the orifice plate 16 filled with magnetic bead liquid deviate from directly below the injection assembly. The orifice plate 16 is then removed.

[0079] In the description of this utility model, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0080] The above are merely preferred embodiments of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application.

Claims

1. An apparatus for filling magnetic bead liquid, characterized in that, The system includes a base (1), on which a stirring mechanism, a filling mechanism, and a moving mechanism are provided. The stirring mechanism includes a stirring tank (5), on which a motor (8) is installed at the top center. The output shaft of the motor (8) passes through the top wall of the stirring tank (5) and is sequentially provided with an upper stirring component, a reciprocating component, and a lower stirring component. The top of the stirring tank (5) is provided with a liquid inlet pipe (7), and the bottom of the stirring tank (5) is provided with a liquid outlet (28). The filling mechanism includes a pressing component, on which an injection component is provided at the execution end of the pressing component. The injection component is connected to the liquid outlet of a peristaltic pump (2), and the liquid inlet of the peristaltic pump (2) is connected to the liquid outlet (28). The moving mechanism is provided with an orifice plate (16) at its upper limit, and the injection component is correspondingly provided with the orifice plate (16).

2. The apparatus for filling magnetic bead liquid according to claim 1, characterized in that, The upper stirring assembly includes a rotating rod two (24) connected to the output shaft of the motor (8). Multiple stirring blades (22) are connected circumferentially on the outer wall of the rotating rod two (24). A scraper (23) is connected to one end of the stirring blade (22) away from the rotating rod two (24). The scraper (23) is in frictional contact with the inner wall of the stirring tank (5).

3. The apparatus for filling magnetic bead liquid according to claim 2, characterized in that, The reciprocating assembly includes a reciprocating threaded rod (25) connected to the bottom end of the rotating rod (24). A convection plate (20) is coaxially threaded onto the reciprocating threaded rod (25). The convection plate (20) is slidably limited on the inner wall of the mixing tank (5). The convection plate (20) has multiple through holes (21), and the cross-section of the through holes (21) is trapezoidal.

4. The apparatus for filling magnetic bead liquid according to claim 3, characterized in that, The lower stirring assembly includes a rotating rod (19) connected to the bottom end of the reciprocating threaded rod (25), and a spiral blade (18) is wound around the rotating rod (19).

5. The apparatus for filling magnetic bead liquid according to claim 1, characterized in that, The pressing assembly includes a slide rod 1 (6) and a slide rod 2 (11) vertically fixedly connected to the top of the base (1). A slide table (9) is slidably sleeved between the slide rod 2 (11) and the slide rod 1 (6). The liquid injection assembly is located on the side of the slide table (9) near the moving mechanism. The lower part of the control lever (10) is hinged to one end of the slide table (9) near the liquid injection assembly. A hinge seat (38) is connected to the base (1). One end of an arc-shaped connecting plate (4) is hinged to the hinge seat (38). The other end of the arc-shaped connecting plate (4) is hinged to the bottom end of the control lever (10). An adjusting block (3) is provided at the lower part of the slide rod 2 (11). A spring 1 (15) is slidably sleeved on the slide rod 2 (11). The top end of the spring 1 (15) abuts against the bottom end of the slide table (9). The other end of the spring 1 (15) abuts against the top end of the adjusting block (3).

6. The apparatus for filling magnetic bead liquid according to claim 5, characterized in that, The injection assembly includes a pressure plate (12) connected to the bottom of the slide (9). Multiple liquid storage tubes (13) are connected at equal intervals at the bottom of the pressure plate (12). The liquid storage tubes (13) are connected to the outlet of the peristaltic pump (2) through pipelines. A flow valve is provided on the pipelines. Multiple injection needle valves are provided at equal intervals at the bottom of the liquid storage tubes (13). The injection needle valves are provided in correspondence with the holes on the orifice plate (16).

7. The apparatus for filling magnetic bead liquid according to claim 6, characterized in that, The injection needle valve includes an injection tube (14) connected to the bottom end of the reservoir tube (13). A needle (33) is slidably disposed inside the injection tube (14). The bottom end of the needle (33) penetrates the bottom wall of the injection tube (14) and is connected to a small ball (34). A valve core (31) is fixedly sleeved on the needle (33). The cross-section of the valve core (31) is conical. The inner bottom wall of the injection tube (14) has an opening adapted to the valve core (31). The conical groove is provided, and multiple injection holes (32) are opened horizontally on the groove wall. A bracket (29) is slidably sleeved on the upper part of the ejector pin (33). The bracket (29) is fixedly connected to the inner wall of the injection tube (14). A second spring (30) is slidably sleeved on the ejector pin (33). The top end of the second spring (30) abuts against the bracket (29), and the bottom end of the second spring (30) abuts against the top end of the valve core (31).

8. The apparatus for filling magnetic bead liquid according to claim 1, characterized in that, The moving mechanism includes a sliding plate (17) slidably disposed on the base (1), and a positioning frame (37) is connected to the top of the sliding plate (17). The perforated plate (16) is positioned on the sliding plate (17) through the positioning frame (37).

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