Staining device for sequencing sample preparation system and sequencing sample preparation system
By designing an automated staining device, the sequencing sample preparation process was streamlined, solving the problems of cumbersome manual operation and contamination risks, improving staining effect and efficiency, and reducing costs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SANSURE BIOTECH INC
- Filing Date
- 2025-05-13
- Publication Date
- 2026-04-24
AI Technical Summary
In the current sequencing sample preparation process, the manual staining process is cumbersome, and it is difficult to accurately control the number of reagent additions and washing cycles, resulting in unstable staining results and risks of sample damage, reagent waste, and contamination.
Design a staining device that includes a test tube rack, a stage, a washing station, and a slide rack module. The device achieves automated staining and washing through a sliding contact structure, reducing manual intervention, and uses multiple washing tanks to reduce the risk of contamination.
It improved the staining effect of sequencing samples, reduced labor costs, shortened the disease diagnosis cycle, increased preparation efficiency and success rate, and reduced the risk of contamination.
Smart Images
Figure CN224163443U_ABST
Abstract
Description
Technical Field
[0001] This application belongs to the technical field of sequencing sample preparation systems, specifically relating to a staining device and a sequencing sample preparation system. Background Technology
[0002] In the preparation of existing sequencing samples (such as spatial transcriptome sequencing samples), the staining process (such as H&E staining) is mostly done manually. However, manual operation of the above process is quite cumbersome, specifically in the following ways: First, the staining process involves multiple manual liquid addition operations, requiring precise control of the progressive replacement of reagents such as hematoxylin and eosin. However, it is difficult to precisely control the liquid addition time and the number of washes, and human error is prone to occur during operation, which can easily lead to unstable staining results. Second, multiple independent washing steps and frequent liquid replacement not only increase reagent consumption, but also easily cause tissue sections to detach or fold, posing a risk of sample damage, and also easily causing reagent waste and contamination. Utility Model Content
[0003] The purpose of this application is to provide a staining device and a sequencing sample preparation system for use in a sequencing sample preparation system. The staining device and the sequencing sample preparation system for use in a sequencing sample preparation system have the advantages of simple structure, which helps to reduce labor costs and improve the staining effect of sequencing samples.
[0004] To achieve the above objectives, a first aspect of this application provides a staining apparatus for a sequencing sample preparation system, the staining apparatus comprising:
[0005] A test tube rack is used to hold multiple reagent tubes, which are used to hold different types of staining reagents.
[0006] A shelf is placed on one side of the test tube rack;
[0007] A cleaning station is located on the side of the shelf away from the test tube rack and includes a cleaning tank and multiple chute assemblies. The cleaning tank has multiple cleaning tanks arranged in sequence and each containing a cleaning medium. The multiple chute assemblies are located above the cleaning tank and correspond to the multiple cleaning tanks. The chute assemblies have vertically distributed chute grooves.
[0008] The slide holder module is used to hold slides containing sequencing sample tissues. The slide holder module forms sliding contact with the top surface of the stage, the top surface of the slide body assembly, and the wall of the slide.
[0009] In this configuration, with the slide holder module in a state where it is slid to the bottom of the trough, both the slide holder module and the slides are vertically distributed and at least some of the slides are immersed in the cleaning medium.
[0010] In the embodiments of this application, multiple cleaning tanks are arranged sequentially along the longitudinal direction. The chute assembly includes a first chute body and a second chute body respectively disposed on the upper walls of the tanks on both sides of the transverse direction of the cleaning tank. A chute is formed on both the first chute body and the second chute body. The chute passes through the transverse direction. A receiving space for accommodating part of the slide holder module is formed between the chute of the first chute body and the chute of the second chute body.
[0011] In the embodiments of this application, the slide holder module includes a holder assembly, a first sliding rod assembly, and a second sliding rod assembly. The holder assembly is used to clamp the slides. The first sliding rod assembly and the second sliding rod assembly are respectively disposed on the lateral sides of the holder assembly and form sliding contact with the top surface of the platform, the top surface of the slide body assembly, and the wall of the slide. The lateral width of the holder assembly is smaller than the lateral width of the cleaning tank.
[0012] In the embodiments of this application, both the first sliding rod assembly and the second sliding rod assembly include a first connecting rod, a second connecting rod, and a third connecting rod. The first connecting rod is distributed laterally and connected to the bracket assembly. The second connecting rod is distributed vertically and connected to the end of the first connecting rod away from the bracket assembly. The third connecting rod is rotatably connected to the end of the second connecting rod away from the first connecting rod. The length of the third connecting rod is less than the length of the first connecting rod.
[0013] In embodiments of this application, the first sliding rod assembly and the second sliding rod assembly each include a first ball and a second ball, the first ball being rotatably mounted on the first connecting rod, and the second ball being rotatably mounted on the third connecting rod.
[0014] In embodiments of this application, the staining apparatus further includes a waste liquid collection platform disposed between the test tube rack and the stage, and a waste liquid collection tank is formed on the waste liquid collection platform.
[0015] In the embodiments of this application, the top surface of the waste liquid collection platform, the top surface of the placement platform, and the top surface of the chute assembly are together formed with a guide groove for guiding the first ball and the second ball.
[0016] In embodiments of this application, the staining apparatus further includes a waste liquid collection platform disposed between the test tube rack and the stage, and a waste liquid collection tank is formed on the waste liquid collection platform.
[0017] In the embodiments of this application, a detachable connection is formed between the waste liquid collection platform and the storage platform.
[0018] In the embodiments of this application, the washing table and the storage table are detachably connected, the washing tank includes a plurality of washing tank sections, the washing tank is formed on the washing tank sections, and a detachable link is formed between two adjacent washing tank sections.
[0019] A second aspect of this application provides a sequencing sample preparation system, which includes the staining device described above for a sequencing sample preparation system.
[0020] As can be seen from the above technical solution, the staining device includes a test tube rack, a stage, a washing stage, and a slide holder module. The stage is located on one side of the test tube rack. The washing stage is located on the side of the stage away from the test tube rack and includes a washing tank and multiple sliding chute assemblies. Multiple washing tanks arranged sequentially and each containing washing medium are formed on the washing tank. Multiple sliding chute assemblies are located above the washing tank and correspond to the multiple washing tanks. Slide chute assemblies have vertically distributed slides. The slide holder module is used to hold slides containing sequencing sample tissues. The slide holder module forms sliding contact with the top surface of the stage, the top surface of the sliding chute assemblies, and the wall of the slide chute. When the slide holder module is in the state of sliding to the bottom of the slide chute, both the slide holder module and the slides are vertically distributed and at least part of the slides are immersed in the washing medium. This staining device has a simple structure. The sliding contact between the slide holder module and the top surface of the stage, the top surface of the slide assembly, and the wall of the slide allows the operator to easily change the position of the slide holder module, which can improve the efficiency of sequencing sample preparation and shorten the disease diagnosis cycle. The distribution of the tube rack, stage, and washing station also helps to streamline the staining process, reduce manual intervention, and improve the success rate of sequencing sample preparation. The multiple washing tanks can reduce the risk of contamination.
[0021] Other features and advantages of the embodiments of this application will be described in detail in the following detailed description section. Attached Figure Description
[0022] The accompanying drawings are provided to further illustrate the embodiments of this application and form part of the specification. They are used together with the following detailed description to explain the embodiments of this application, but do not constitute a limitation on the embodiments of this application. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without any inventive effort. In the drawings:
[0023] Figure 1 This is a schematic diagram of the dyeing apparatus in an embodiment of this application.
[0024] Explanation of reference numerals in the attached figures
[0025] 1-Test tube rack; 101-Reagent tube mounting cavity; 2-Placement stage; 3-Washing stage; 301-Washing tank body; 302-Slide body assembly; 303-Washing tank; 304-Slide; 305-First slide body; 306-Second slide body; 307-Washing tank section; 4-Slide holder module; 401-Holding assembly; 402-First sliding rod assembly; 403-Second sliding rod assembly; 404-First connecting rod; 405-Second connecting rod; 406-Third connecting rod; 407-First ball bearing; 408-Second ball bearing; 5-Slide; 6-Waste liquid collection stage; 601-Waste liquid collection tank; 7-Slide holder. Detailed Implementation
[0026] The specific embodiments of this application will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit this application.
[0027] Embodiments of this application provide a staining apparatus for a sequencing sample preparation system, such as... Figure 1 As shown, the staining apparatus includes:
[0028] Test tube rack 1 is used to hold multiple reagent tubes, which are used to hold different types of staining reagents.
[0029] The shelf 2 is set on one side of the test tube rack 1;
[0030] The cleaning table 3 is located on the side of the platform 2 away from the test tube rack 1 and includes a cleaning tank 301 and multiple sliding chute assemblies 302. Multiple cleaning tanks 303 are formed on the cleaning tank 301, arranged in sequence and each containing a cleaning medium. Multiple sliding chute assemblies 302 are all located above the cleaning tank 301 and correspond to the multiple cleaning tanks 303. Sliding chute 304 are formed on the sliding chute assemblies 302 along the vertical direction.
[0031] The slide holder module 4 is used to hold and place the slides 5 containing sequencing sample tissues. The slide holder module 4 forms sliding contact with the top surface of the stage 2, the top surface of the slide body assembly 302, and the wall of the slide 304.
[0032] When the slide holder module 4 is slid to the bottom of the slide groove 304, both the slide holder module 4 and the slides 5 are vertically distributed and at least some of the slides 5 are immersed in the cleaning medium.
[0033] Specifically, in this embodiment, the sequencing sample can be a spatial transcriptome sequencing sample. The staining device is used to perform H&E staining (i.e., hematoxylin-eosin staining) on the spatial transcriptome sequencing sample tissue during the preparation of the spatial transcriptome sequencing sample. The test tube rack 1 is distributed horizontally and forms multiple reagent tube mounting cavities 101. Multiple reagent tubes can be placed one-to-one on the reagent tube mounting cavities 101. In this embodiment, the multiple reagent tubes can be selected as three and are respectively loaded with hematoxylin, eosin, and reverse blue solution as staining reagents. The stage 2 is set on one longitudinal side of the test tube rack 1. The slide holder module 4 is set horizontally and can slide longitudinally on the top surface of the stage 2. The slide holder module 4 is provided with multiple slide clips 7. The slides 5 can be clamped horizontally on the slide holder module 4 through the slide clips 7. The sequencing sample tissue on the slide 5 is the sequencing sample tissue that has been dewaxed. The cleaning tank 301 is recessed from top to bottom to form a cleaning tank 303. Multiple cleaning tanks 303 are arranged parallel to each other in the longitudinal direction, and individual cleaning tanks 303 are distributed in the transverse direction. In this embodiment, the cleaning medium can be pure water. The top surface of the slide assembly 302 is flush with the top surface of the platform 2. Further, in this embodiment, the number of cleaning tanks 303 is six, including a first cleaning tank, a second cleaning tank, a third cleaning tank, a fourth cleaning tank, a fifth cleaning tank, and a sixth cleaning tank arranged sequentially in the longitudinal direction.
[0034] Operators can use the staining device in this embodiment to stain the sequencing sample tissue on slide 5 using the following steps (between performing the following steps, multiple reagent tubes contain hematoxylin, eosin, and reverse blue staining reagents, and six washing tanks 303 each contain pure water):
[0035] Step S101: The dewaxed glass slide 5 is clamped onto the glass slide holder module 4;
[0036] Step S102: Apply a horizontal force (horizontal push or horizontal pull) to the slide holder module 4 to slide the slide holder module 4 to a position close to the test tube rack 1, add 1 mL of hematoxylin to the sequencing sample tissue on each slide 5 and keep it at room temperature for 3 min;
[0037] Step S103: Perform a first removal process on the excess hematoxylin on each slide 5;
[0038] Step S104: Apply a horizontal force (horizontal push or horizontal pull) to the slide holder module 4 to make the slide holder module 4 slide from the top surface of the stage 2 to the top surface of the slide body assembly 302 corresponding to the first cleaning tank. Then apply a flipping force to the slide holder module 4 to make the slide holder module 4 flip from horizontal distribution to vertical distribution and enter the slide 304. After the slide holder module 4 enters the slide 304 corresponding to the first cleaning tank, repeatedly apply a downward vertical force or an upward vertical force to the slide holder module 4 to make the slide holder module 4 slide vertically repeatedly in the slide 304. During the process of the slide holder module 4 sliding vertically repeatedly in the slide 304, part of the slide 5 (such as 2 / 3 slide 5) on the slide holder module 4 is repeatedly (such as 5 times) immersed in the pure water of the first cleaning tank to clean the slide 5.
[0039] Step S105: Apply an upward vertical force to the slide holder module 4 to make the slide holder module 4 slide out of the slide groove 304 corresponding to the first cleaning tank. Then apply a horizontal force (horizontal pushing or horizontal pulling force) to the slide holder module 4 to make the slide holder module 4 slide from the top surface of the slide groove body assembly 302 corresponding to the first cleaning tank to the top surface of the slide groove body assembly 302 corresponding to the second cleaning tank. Then apply a flipping force to the slide holder module 4 to flip the slide holder module 4 from a horizontal distribution to a vertical distribution and enter the second cleaning tank. In the slide 304 corresponding to the washing tank, after the slide holder module 4 enters the slide 304 corresponding to the second washing tank, a downward vertical force or an upward vertical force is repeatedly applied to the slide holder module 4 so that the slide holder module 4 slides vertically repeatedly in the slide 304. During the process of the slide holder module 4 sliding vertically repeatedly in the slide 304, a portion of the slide 5 (such as 2 / 3 of the slide 5) on the slide holder module 4 is repeatedly (such as 15 times) immersed in the pure water of the second washing tank to clean the slide 5.
[0040] Step S106: Apply an upward vertical force to the slide holder module 4 to make the slide holder module 4 slide out of the slide groove 304 corresponding to the second cleaning tank. Then apply a horizontal force (horizontal pushing or horizontal pulling force) to the slide holder module 4 to make the slide holder module 4 slide from the top surface of the slide groove body assembly 302 corresponding to the second cleaning tank to the top surface of the slide groove body assembly 302 corresponding to the third cleaning tank. Then apply a flipping force to the slide holder module 4 to flip the slide holder module 4 from a horizontal distribution to a vertical distribution and enter the third cleaning tank. In the slide 304 corresponding to the washing tank, after the slide holder module 4 enters the slide 304 corresponding to the third washing tank, a downward vertical force or an upward vertical force is repeatedly applied to the slide holder module 4 so that the slide holder module 4 slides vertically repeatedly in the slide 304. During the process of the slide holder module 4 sliding vertically repeatedly in the slide 304, a portion of the slide 5 (such as 2 / 3 of the slide 5) on the slide holder module 4 is repeatedly (such as 15 times) immersed in the pure water of the third washing tank to clean the slide 5.
[0041] Step S107: Perform a reset operation on the slide holder module 4 so that the slide holder module 4 slides out of the slide groove 304 corresponding to the third cleaning tank and slides to a position close to the test tube rack 1. Add 1 mL of inverted blue solution to the sequencing sample tissue on each slide 5 and keep it at room temperature for 1 min.
[0042] Step S108: Perform a second removal process on the excess blue solution on each slide 5;
[0043] Step S109: Apply a horizontal force (horizontal push or horizontal pull) to the slide holder module 4 to make the slide holder module 4 slide from the top surface of the stage 2 to the top surface of the slide body assembly 302 corresponding to the fourth cleaning tank. Then apply a flipping force to the slide holder module 4 to make the slide holder module 4 flip from horizontal distribution to vertical distribution and enter the slide 304. After the slide holder module 4 enters the slide 304 corresponding to the fourth cleaning tank, repeatedly apply a downward vertical force or an upward vertical force to the slide holder module 4 to make the slide holder module 4 slide vertically repeatedly in the slide 304. During the process of the slide holder module 4 sliding vertically repeatedly in the slide 304, a portion of the slides 5 on the slide holder module 4 (such as 2 / 3 of the slides 5) are repeatedly (such as 15 times) immersed in the pure water of the fourth cleaning tank to clean the slides 5.
[0044] Step S110: Perform a reset operation on the slide holder module 4 again so that the slide holder module 4 slides out of the slide groove 304 corresponding to the fourth cleaning tank and slides to a position close to the test tube rack 1. Add eosin (the amount of eosin is enough to immerse the sequencing sample tissue) to the sequencing sample tissue on each slide 5 and keep it at room temperature for 2 minutes.
[0045] Step S111: Perform a third removal process on the excess eosin on each slide 5;
[0046] Step S112: Apply a horizontal force (horizontal push or horizontal pull) to the slide holder module 4 to make the slide holder module 4 slide from the top surface of the stage 2 to the top surface of the slide body assembly 302 corresponding to the fifth cleaning tank. Then apply a flipping force to the slide holder module 4 to make the slide holder module 4 flip from horizontal distribution to vertical distribution and enter the slide 304. After the slide holder module 4 enters the slide 304 corresponding to the fifth cleaning tank, repeatedly apply a downward vertical force to the slide holder module 4 to make the slide holder module 4 slide to the bottom of the slide 304. The sequencing sample tissue on the slide 5 is completely immersed in the pure water of the fifth cleaning tank for 30 seconds.
[0047] Step S113: Apply a horizontal force (horizontal push or horizontal pull) to the slide holder module 4 to make the slide holder module 4 slide from the top surface of the stage 2 to the top surface of the slide body assembly 302 corresponding to the sixth cleaning tank. Then apply a flipping force to the slide holder module 4 to flip it from a horizontal distribution to a vertical distribution and enter the slide 304. After the slide holder module 4 enters the slide 304 corresponding to the sixth cleaning tank, repeatedly apply a downward vertical force or an upward vertical force to the slide holder module 4 to make the slide holder module 4 slide vertically repeatedly in the slide 304. During the process of the slide holder module 4 sliding vertically repeatedly in the slide 304, a portion of the slide 5 on the slide holder module 4 (such as 2 / 3 slide 5) is repeatedly (e.g., 15 times) immersed in the pure water of the sixth cleaning tank to clean the slide 5. At this point, the staining treatment of the sequencing sample tissue on the slide 5 is completed.
[0048] The staining apparatus in this embodiment has a simple structure. The sliding contact between the slide holder module 4 and the top surface of the stage 2, the top surface of the slide body assembly 302, and the wall of the slide 304 allows the operator to easily change the position of the slide holder module 4, which can improve the efficiency of sequencing sample preparation and shorten the disease diagnosis cycle. The distribution of the test tube rack 1, stage 2, and washing station 3 also helps to streamline the staining process, reduce manual intervention, and improve the success rate of sequencing sample preparation. The multiple washing tanks 303 can reduce the risk of contamination.
[0049] In one embodiment of this application, a plurality of cleaning tanks 303 are arranged sequentially along the longitudinal direction. The chute assembly 302 includes a first chute body 305 and a second chute body 306 respectively disposed on the upper walls of the tanks on both sides of the transverse direction of the cleaning tank 303. A groove 304 is formed on both the first chute body 305 and the second chute body 306. The groove 304 extends transversely. A receiving space for accommodating part of the slide holder module 4 is formed between the groove 304 of the first chute body 305 and the groove 304 of the second chute body 306.
[0050] Specifically, both the first slide body 305 and the second slide body 306 are vertically arranged and each includes a first slide 304 portion and a second slide 304 portion. The first slide 304 portion and the second slide 304 portion are spaced apart in the longitudinal direction. The slide 304 is formed between the first slide 304 portion and the second slide 304 portion. The top surfaces of the first slide 304 portion, the second slide 304 portion, and the top surface of the stage 2 are flush. The overall width of the slide holder module 4 is greater than the width between the first slide body 305 and the second slide body 306. After the slide holder module 4 slides over the top surface of the first slide 304 portion or the top surface of the second slide 304 portion and enters the slide 304, the middle part of the slide holder module 4 and the slide 5 on it are in the receiving space. When the slide holder module 4 moves vertically repeatedly in the slide 304, the middle part of the slide holder module 4 and the slide 5 on it move vertically repeatedly in the receiving space.
[0051] In one embodiment of this application, the slide holder module 4 includes a holder assembly 401, a first sliding rod assembly 402, and a second sliding rod assembly 403. The holder assembly 401 is used to clamp the slide 5. The first sliding rod assembly 402 and the second sliding rod assembly 403 are respectively disposed on the lateral sides of the holder assembly 401 and form sliding contact with the top surface of the stage 2, the top surface of the slide body assembly 302, and the wall of the slide 304. The lateral width of the holder assembly 401 is smaller than the lateral width of the cleaning tank 303.
[0052] Specifically, the mounting assembly 401 includes a first rod, a second rod, and multiple third rods. The first and second rods are distributed laterally and spaced apart longitudinally. The two ends of the third rods are connected to the first and second rods, respectively. The multiple third rods are staggered between the first and second rods and together form a grid. The first, second, and third rods together form a slide clamping area for clamping the slides 5. The slide clamp 7 is rotatably mounted on the first or second rod. After the first sliding rod assembly 402 and the second sliding rod assembly 403 slide into the respective grooves 304 of the first and second sliding groove bodies 305 and 306, the mounting assembly 401 and the slides 5 on it are located in the receiving space.
[0053] In one embodiment of this application, both the first sliding rod assembly 402 and the second sliding rod assembly 403 include a first connecting rod 404, a second connecting rod 405, and a third connecting rod 406. The first connecting rod 404 is distributed laterally and connected to the bracket assembly 401. The second connecting rod 405 is distributed vertically and connected to the end of the first connecting rod 404 away from the bracket assembly 401. The third connecting rod 406 is rotatably connected to the end of the second connecting rod 405 away from the first connecting rod 404. The length of the third connecting rod 406 is less than the length of the first connecting rod 404.
[0054] Specifically, the first connecting rod 404 is connected to the first rod body, and part of the first connecting rod 404 protrudes laterally from the platform 2 and the washing table 3. When the third connecting rod 406 is rotated to a position parallel to the first connecting rod 404, there is a gap between the third connecting rod 406 and the mounting assembly 401. When the slide 5 mounting assembly 401 needs to slide longitudinally, the operator rotates the third connecting rod 406 to a position parallel to the first connecting rod 404. Both the first connecting rod 404 and the third connecting rod 406 can form sliding contact with the top surface of the platform 2, the top surface of the slide chute assembly 302, and the wall of the slide chute 304. The above arrangement can prevent the slide mounting module 4 from flipping when it moves longitudinally in the horizontal plane. When the slide mounting module 4 needs to move vertically, the operator rotates the third connecting rod 406 to a position parallel to the first connecting rod 404. The vertical position of the connecting rod 404 prevents interference between the third connecting rod 406 and the slide body assembly 302 when the slide holder module 4 moves vertically. Then, the first connecting rod 404 slides into the slide 304. Furthermore, when the first connecting rod 404 moves in the slide 304, the second rod is located below the first rod. The above arrangement allows the first connecting rod 404 to slide to the lowest end of the slide 304, thereby allowing the holder assembly 401 and the slides 5 on it to be immersed in pure water as much as possible to complete the cleaning of the slides 5.
[0055] In one embodiment of this application, both the first sliding rod assembly 402 and the second sliding rod assembly 403 further include a first ball bearing 407 and a second ball bearing 408. The first ball bearing 407 is rotatably mounted on the first connecting rod 404, and the second ball bearing 408 is rotatably mounted on the third connecting rod 406. Specifically, the first ball bearing 407 and the second ball bearing 408 can roll on the top surface of the shelf 2, the top surface of the slide body assembly 302, and the groove wall of the slide 304. This arrangement further improves the sliding convenience of the slide holder module 4 and helps to further reduce labor intensity.
[0056] In one embodiment of this application, the staining apparatus further includes a waste liquid collection platform 6 disposed between the test tube rack 1 and the stage 2, and a waste liquid collection tank 601 is formed on the waste liquid collection platform 6.
[0057] Specifically, the top surface of the waste liquid collection platform 6 is flush with the top surface of the platform 2. The top surface of the waste liquid collection platform 6 is recessed downwards to form a waste liquid collection tank 601. The first sliding rod assembly 402 and the second sliding rod assembly 403 form a rolling contact with the top surface of the waste liquid collection platform 6, that is, the first ball bearing 407 and the second ball bearing 408 can move the holder assembly 401 and the glass slide 5 on it to the top of the waste liquid collection tank 601 so that the operator can add staining reagent to the sequencing sample tissue. Further, in steps S103, S108 and S111, when removing excess staining reagent from the glass slide 5, the operator applies a flipping force to the glass slide holder module 4 so that one longitudinal side of the glass slide holder module 4 flips relative to the other longitudinal side of the glass slide holder module 4, so that the excess staining reagent on the glass slide 5 flows into the waste liquid collection tank 601, realizing centralized management of waste liquid and reducing the risk of contamination.
[0058] In one embodiment of this application, the top surface of the waste liquid collection platform 6, the top surface of the placement platform 2, and the top surface of the slide body assembly 302 are jointly formed with a guide groove for guiding the first ball 407 and the second ball 408. The guide groove is distributed longitudinally. When the first ball 407 and the second ball 408 roll in the guide groove, the slide holder module 4 moves longitudinally, which improves the stability of the movement trajectory of the slide holder module 4.
[0059] In one embodiment of this application, the waste liquid collection platform 6 and the storage platform 2 are detachably connected.
[0060] Specifically, a first groove is formed on the side wall of the waste liquid collection platform 6 facing the placement platform 2, with the opening of the first groove facing the placement platform 2. A first protrusion is formed on the side wall of the placement platform 2 facing the waste liquid collection platform 6 for engaging with the first groove. By engaging the first protrusion into the first groove, the waste liquid collection platform 6 and the placement platform 2 can be connected. By removing the first protrusion from the first groove, the waste liquid collection platform 6 and the placement platform 2 can be disassembled. This arrangement facilitates targeted repair or replacement of the waste liquid collection platform 6 or the placement platform 2 when damaged. It also allows for increasing or decreasing the number of waste liquid collection platforms 6 and the placement platform 2 according to actual needs, which is beneficial for expanding the applicability of the staining device and promoting the miniaturization and modularization of staining devices and sequencing sample preparation systems, thereby reducing the cost of high-throughput experiments. Furthermore, in this embodiment, the waste liquid collection platform 6 and the placement platform 2 can also be connected by other connection methods besides the first protrusion and the first groove, such as a hinge locking assembly or a door bolt assembly.
[0061] In one embodiment of this application, the washing table 3 and the storage table 2 are detachably connected. The washing tank 301 includes a plurality of washing tank sections 307, and the washing tank 303 is formed on the washing tank section 307. A detachable connection is formed between two adjacent washing tank sections 307.
[0062] Specifically, a second groove is formed on the side wall of the washing station 3 facing the placement platform 2, with the opening of the second groove facing the placement platform 2. A second protrusion is formed on the side wall of the washing station 3 facing the waste liquid collection platform 6 for engaging with the second groove. By engaging the second protrusion into the second groove, the washing station 3 and the placement platform 2 can be connected. By removing the second protrusion from the second groove, the washing station 3 and the placement platform 2 can be disassembled. This design facilitates targeted repair or replacement of the washing station 3 or the placement platform 2 when damaged. It also allows for increasing or decreasing the number of washing stations 3 and the placement platform 2 according to actual needs, which is beneficial for expanding the applicability and functionality of the staining device. Furthermore, it promotes the miniaturization and modularization of staining devices and sequencing sample preparation systems, reducing the cost of high-throughput experiments.
[0063] Furthermore, in this embodiment, the washing table 3 and the storage table 2 can also be connected by other connection methods besides the second protrusion and the second groove, such as a hinge locking assembly or a door bolt assembly.
[0064] Similarly, one of two adjacent cleaning tank sections 307 has a vertically distributed third groove, and the other has a third protrusion on its side wall for engaging with the third groove. Engaging the third protrusion into the third groove connects the two cleaning tank sections 307, and removing the third protrusion from the third groove allows for disassembly. This design facilitates targeted repair or replacement of a single cleaning tank section 307 when damaged, and also allows for increasing or decreasing the number of cleaning tank sections 307 according to actual needs. This expands the applicability and functionality of the staining device, and promotes the miniaturization and modularization of staining devices and sequencing sample preparation systems, reducing the cost of high-throughput experiments. Furthermore, in this embodiment, the two cleaning tank sections 307 can also be connected using other methods besides the third protrusion and third groove, such as hinge locking assemblies or door bolt assemblies.
[0065] Another embodiment of this application provides a sequencing sample preparation system, which includes the staining device for sequencing sample preparation system described in the above embodiments.
[0066] In the description of this application, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.
[0067] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between components; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.
[0068] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.
[0069] Although embodiments of this application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this application. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this application.
Claims
1. A staining apparatus for a sequencing sample preparation system, characterized in that, The staining apparatus includes: Test tube rack (1) for holding multiple reagent tubes, the multiple reagent tubes being used to hold different types of staining reagents respectively; A shelf (2) is provided on one side of the test tube rack (1); A cleaning station (3) is located on the side of the platform (2) away from the test tube rack (1) and includes a cleaning tank (301) and a plurality of chute assemblies (302). The cleaning tank (301) has a plurality of cleaning tanks (303) arranged in sequence and each containing a cleaning medium. The plurality of chute assemblies (302) are located above the cleaning tank (301) and correspond to the plurality of cleaning tanks (303). The chute assemblies (302) have vertically distributed chutes (304). A slide holder module (4) is used to hold a slide (5) containing sequencing sample tissue. The slide holder module (4) forms a sliding contact with the top surface of the stage (2), the top surface of the slide body assembly (302), and the wall of the slide (304). When the slide holder module (4) is slid to the bottom of the slide groove (304), both the slide holder module (4) and the slides (5) are vertically distributed and at least part of the slides (5) are immersed in the cleaning medium.
2. The staining apparatus for a sequencing sample preparation system according to claim 1, characterized in that, Multiple cleaning tanks (303) are arranged longitudinally in sequence. The chute assembly (302) includes a first chute body (305) and a second chute body (306) respectively disposed on the upper walls of the tanks on both sides of the lateral direction of the cleaning tank (303). Both the first chute body (305) and the second chute body (306) have grooves (304) formed on them. The grooves (304) extend laterally. A receiving space for accommodating part of the slide holder module (4) is formed between the grooves (304) of the first chute body (305) and the grooves (304) of the second chute body (306).
3. The staining apparatus for a sequencing sample preparation system according to claim 1, characterized in that, The slide holder module (4) includes a holder assembly (401), a first sliding rod assembly (402), and a second sliding rod assembly (403). The holder assembly (401) is used to clamp the slide (5). The first sliding rod assembly (402) and the second sliding rod assembly (403) are respectively disposed on the lateral sides of the holder assembly (401) and form sliding contact with the top surface of the stage (2), the top surface of the slide body assembly (302), and the wall of the slide (304). The lateral width of the holder assembly (401) is smaller than the lateral width of the cleaning tank (303).
4. The staining apparatus for a sequencing sample preparation system according to claim 3, characterized in that, Both the first sliding rod assembly (402) and the second sliding rod assembly (403) include a first connecting rod (404), a second connecting rod (405), and a third connecting rod (406). The first connecting rod (404) is distributed laterally and connected to the bracket assembly (401). The second connecting rod (405) is distributed vertically and connected to the end of the first connecting rod (404) away from the bracket assembly (401). The third connecting rod (406) is rotatably connected to the end of the second connecting rod (405) away from the first connecting rod (404). The length of the third connecting rod (406) is less than the length of the first connecting rod (404).
5. The staining apparatus for a sequencing sample preparation system according to claim 4, characterized in that, The first sliding rod assembly (402) and the second sliding rod assembly (403) each include a first ball (407) and a second ball (408). The first ball (407) is rotatably sleeved on the first connecting rod (404), and the second ball (408) is rotatably sleeved on the third connecting rod (406).
6. The staining apparatus for a sequencing sample preparation system according to claim 5, characterized in that, The staining apparatus also includes a waste liquid collection platform (6) disposed between the test tube rack (1) and the stage (2), and a waste liquid collection tank (601) is formed on the waste liquid collection platform (6).
7. The staining apparatus for a sequencing sample preparation system according to claim 6, characterized in that, The top surface of the waste liquid collection platform (6), the top surface of the placement platform (2), and the top surface of the chute assembly (302) are together formed with a guide groove for guiding the first ball (407) and the second ball (408).
8. The staining apparatus for a sequencing sample preparation system according to claim 6, characterized in that, The waste liquid collection platform (6) and the storage platform (2) are detachably connected.
9. The staining apparatus for a sequencing sample preparation system according to any one of claims 1-8, characterized in that, The cleaning table (3) is detachably connected to the storage table (2). The cleaning tank (301) includes a plurality of cleaning tank sections (307). The cleaning tank (303) is formed on the cleaning tank section (307). A detachable link is formed between two adjacent cleaning tank sections (307).
10. A sequencing sample preparation system, characterized in that, The sequencing sample preparation system includes a staining device for a sequencing sample preparation system according to any one of claims 1-9.