Detection kit for detecting content of chlorogenic acid in sample

Abstract

The utility model relates to the technical field of traditional Chinese medicinal material detection, in particular to a detection kit for detecting the content of chlorogenic acid in a sample. The kit comprises a kit body, a lifting device is arranged on the inner surface of the kit body, the lifting device comprises a concentric-square-shaped frame, and the concentric-square-shaped frame is connected with the inner surface of the kit body in a sliding mode. The problem that when a worker needs to use reagents in a kit body to detect the content of chlorogenic acid in samples such as Chinese patent medicines, traditional Chinese medicinal materials, health care products and cosmetics, the worker is difficult to observe the remaining amount of each reagent in the kit body or whether the reagent is opened is solved. The problems that the chlorogenic acid content detection reagent taken out by a worker at a time is insufficient or a new chlorogenic acid content detection reagent is unconsciously opened, so that the worker needs to take the medium chlorogenic acid content detection reagent for multiple times or the medium chlorogenic acid content detection reagent is wasted are solved.

Description

Technical Field

[0001] This utility model relates to the field of traditional Chinese medicine detection technology, and in particular to a detection kit for detecting the chlorogenic acid content in a sample. Background Technology

[0002] Chlorogenic acid is an organic compound and one of the main effective antibacterial and antiviral pharmacological components of honeysuckle. While it has broad-spectrum antibacterial activity, it can be inactivated by proteins in vivo. Therefore, when detecting the chlorogenic acid content in samples such as traditional Chinese medicine preparations, medicinal herbs, health products, and cosmetics, detection reagents are often required. These reagents are typically stored in kits, which are boxes used to hold chemical reagents for detecting chemical components, drug residues, virus types, etc. These kits come in various sizes and are often made of paper. High-performance liquid chromatography (HPLC), ultraviolet-visible spectrophotometry (UV-Vis spectrophotometry), and thin-layer chromatography (TLC) are commonly used methods for detecting chlorogenic acid content.

[0003] The principle of high performance liquid chromatography (HPLC): Chlorogenic acid in the sample is carried by the mobile phase through a chromatographic column packed with a stationary phase. Due to the different interactions between chlorogenic acid and the stationary and mobile phases, it is separated from other components. Then, its peak area is detected at a specific wavelength by an ultraviolet detector, and quantification is performed by comparing the peak area with that of a standard.

[0004] The principle of ultraviolet-visible spectrophotometry: Chlorogenic acid has a characteristic absorption peak in the ultraviolet-visible region. According to the Lambert-Beer law, the content of chlorogenic acid is calculated by measuring the absorbance of the sample solution at a certain wavelength.

[0005] Thin-layer chromatography principle: The adsorbent is evenly spread on a glass plate to form a thin layer as the stationary phase. The sample is spotted at one end of the thin-layer plate and placed in a developing tank containing the developing solvent. The developing solvent moves the sample. Chlorogenic acid is separated from other components due to the different partition coefficients in the stationary phase and the developing solvent. Its position and spot size are determined by color development, thereby performing qualitative and semi-quantitative analysis.

[0006] Chinese patent application CN202320099938.8 discloses a rapid test kit. The key technical points of the kit are: the sealing cap is flipped up to be perpendicular to the box body, then the test reagent paper is placed in the placement slot, and after opening the door panel to take out other test tools, the test reagent paper can be used for rapid testing. A garbage bag is placed between the two sets of first and second long plates, so that the garbage bag is open, making it easy for the testing personnel to throw the waste test reagent paper and disposable test tools into the garbage bag for storage, avoiding the pollution of the environment by littering waste test reagent paper and disposable test tools. After the test is completed, the testing personnel collect the garbage bag.

[0007] Regarding the aforementioned and existing related technologies, the inventors believe that the following defects often exist: High-performance liquid chromatography, ultraviolet-visible spectrophotometry, and thin-layer chromatography are commonly used methods for detecting chlorogenic acid content. Chlorogenic acid reagent kits come in various sizes and are often made of paper. When staff need to use the reagents in the kit to detect the chlorogenic acid content in samples such as traditional Chinese medicine, medicinal herbs, health products, and cosmetics, it is difficult for staff to observe the remaining amount of each chlorogenic acid detection reagent or whether the reagent has been opened. This leads to staff not taking out enough chlorogenic acid detection reagent at one time or unintentionally opening new reagents, resulting in staff needing to take out chlorogenic acid detection reagent multiple times or wasting chlorogenic acid detection reagent. Therefore, to address the above problems, a detection kit for detecting the chlorogenic acid content in samples is proposed. Utility Model Content

[0008] The purpose of this invention is to address the shortcomings of existing technologies where staff may not be able to take out enough reagents at once or may unintentionally open new reagents, resulting in staff having to take out reagents multiple times or wasting reagents. Therefore, this invention proposes a reagent kit for detecting the chlorogenic acid content in samples.

[0009] To achieve the above objectives, the present invention adopts the following technical solution: a detection kit for detecting chlorogenic acid content in a sample, comprising a kit body, a lifting device provided on the inner surface of the kit body, the lifting device including a U-shaped frame, the U-shaped frame being slidably connected to the inner surface of the kit body, five chlorogenic acid standard solutions of different concentrations being slidably inserted in the U-shaped frame, an enzyme label being slidably inserted in the U-shaped frame, an anti-chlorogenic acid monoclonal antibody solution being slidably inserted in the U-shaped frame, a colorimetric reagent being slidably inserted in the U-shaped frame, a stop solution being slidably inserted in the U-shaped frame, a sliding block being fixedly connected to one side of the U-shaped frame, a sliding groove being opened on one side of the kit body, the sliding block being slidably connected to the inner surface of the sliding groove of the kit body, a push-pull plate being fixedly connected to the side of the sliding block away from the U-shaped frame, the push-pull plate being slidably connected to the kit body on the side near the sliding block, a plurality of stabilizing rings being fixedly connected to the inner surface of the U-shaped frame, and an enzyme-labeled plate coated with chlorogenic acid A antigen or goat anti-mouse secondary antibody being adhered to one side of the kit body.

[0010] The aforementioned components achieve the following effect: By setting up a lifting device, it facilitates the lifting of all chlorogenic acid content detection reagents within the reagent kit, making it easier for staff to observe and select. This avoids situations where staff, when using reagents from the reagent kit to test the chlorogenic acid content in samples such as traditional Chinese medicine, medicinal herbs, health products, and cosmetics, find it difficult to observe the remaining amount of each chlorogenic acid detection reagent or whether the reagents have been turned on. This prevents staff from taking out insufficient chlorogenic acid detection reagents at once or unintentionally turning on new reagents, resulting in the need for multiple chlorogenic acid detection reagents or wasting reagents. This improves the practicality of the device.

[0011] Preferably, a fixing rope is fixedly connected to the upper surface of the push-pull plate, and a hook is fixedly connected to one side of the reagent kit body.

[0012] The aforementioned components achieve the following effect: they secure the paperclip, allowing staff to free their hands to remove reagents from the paperclip, thus improving the device's convenience.

[0013] Preferably, a first spring is fixedly connected to the side of the push-pull plate away from the fixed rope, and a semi-circular plate is fixedly connected to the end of the first spring away from the push-pull plate. The semi-circular plate is fixedly connected to one side of the reagent kit body.

[0014] The effect achieved by the above components is that the first spring automatically resets the reagents on the retractable rack, thereby reducing the number of steps required for the operator to operate the device and improving the operator's work efficiency.

[0015] Preferably, a soft pad is fixedly connected to the inner surface of the stabilizing ring, and the size of the soft pad is adapted to the size of the stabilizing ring.

[0016] The effect achieved by the above components is that the soft pad reduces the collision between the stabilizing ring and the reagents on the rack, thereby improving the lifespan of the reagent kit body and the reagents on the rack.

[0017] Preferably, the inner surface of the reagent kit body is provided with a protective device, which includes four L-shaped plates. The four L-shaped plates are fixedly connected to the inner surface of the reagent kit body. Four protective blocks are slidably inserted into the reagent kit body. The surfaces of the four protective blocks are slidably connected to the four L-shaped plates respectively. A protective plate is fixedly connected to one side of each of the four protective blocks. A winding rope is fixedly connected to both sides of each protective plate. A winding rod is fixedly connected to both sides of the reagent kit body. The arc surface of the winding rope is slidably connected to the winding rod.

[0018] The aforementioned components achieve the following effect: by setting up protective devices, the lower surface and four corners of the reagent kit body are supported and protected, preventing the reagent kit body from being squeezed by external forces or dropped on the ground during transportation, which could cause deformation of the reagent kit body and damage to the reagents inside the reagent kit body, thereby improving the safety of the device.

[0019] Preferably, the four L-shaped plates are grouped in pairs, and two support rods are fixedly connected to the side of each pair of L-shaped plates that are close to each other.

[0020] The aforementioned components achieve the following effect: the support rod protects the internal structure of the reagent kit, preventing damage to the reagents inside the kit when the kit is bumped against a sharp corner (where the corner size is smaller than the kit size), thus preventing the kit from being unusable by staff.

[0021] Preferably, four rubber stoppers are fixedly connected to one side of the reagent kit body, and the size of the rubber stoppers is adapted to the size of the L-shaped plate.

[0022] The effect achieved by the above components is that the rubber stopper enhances the stability of the reagent kit body when closed, preventing the reagent kit body from opening automatically and causing the reagents inside the reagent kit body to fall out.

[0023] In summary, the beneficial effects of this utility model are as follows:

[0024] 1. In this utility model, by setting up a lifting device, it is possible for staff to easily pull up all the chlorogenic acid content detection reagents in the reagent kit body, making it convenient for staff to observe and select. This avoids the situation where staff need to use the chlorogenic acid content detection reagents in the reagent kit body to detect the chlorogenic acid content in samples such as traditional Chinese medicine, Chinese medicinal materials, health products, and cosmetics, but it is difficult to observe the remaining amount of each chlorogenic acid content detection reagent in the reagent kit body or whether the chlorogenic acid content detection reagent has been turned on. This can lead to staff not taking out enough chlorogenic acid content detection reagents at one time or unintentionally turning on new chlorogenic acid content detection reagents, resulting in staff having to take out chlorogenic acid content detection reagents multiple times or wasting chlorogenic acid content detection reagents. This improves the practicality of the device.

[0025] 2. In this utility model, by setting up a protective device, the lower surface and four corners of the reagent kit body are supported and protected, which avoids the reagent kit body from being squeezed by external forces or dropped on the ground during transportation, which would cause deformation of the reagent kit body and damage to the reagent kit body and the reagents inside the reagent kit body, thereby improving the safety of the device. Attached Figure Description

[0026] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0027] Figure 2 In this utility model Figure 1 Side view;

[0028] Figure 3 This is a schematic diagram of the lifting device in this utility model;

[0029] Figure 4 In this utility model Figure 3 Enlarged view of point A;

[0030] Figure 5 This is a schematic diagram of the protective device in this utility model;

[0031] Figure 6 In this utility model Figure 2 Enlarged view of point B.

[0032] Legend: 1. Reagent kit body; 2. Pull-out device; 3. Protective device; 4. Chlorogenic acid standard solutions of different concentrations; 5. Enzyme label; 6. Anti-chlorogenic acid monoclonal antibody solution; 7. Chromogenic reagent; 8. Stop solution; 9. Enzyme-labeled plate coated with chlorogenic acid A antigen or goat anti-mouse secondary antibody; 21. Ring frame; 22. Sliding block; 23. Push-pull plate; 24. Stabilizing ring; 25. Fixing rope; 26. Hook; 27. First spring; 28. Semicircular plate; 29. ​​Soft pad; 31. L-shaped plate; 32. Protective block; 33. Protective plate; 34. Winding rope; 35. Winding rod; 36. Support rod; 37. Rubber stopper. Detailed Implementation

[0033] Reference Figure 1As shown, this utility model provides a technical solution: a test kit for detecting chlorogenic acid content in samples, including a kit body 1. The inner surface of the kit body 1 is provided with a lifting device 2. By setting the lifting device 2, it is possible for staff to easily pull up all the chlorogenic acid content detection reagents in the kit body 1, making it convenient for staff to observe and select. This avoids the situation where staff need to use the reagents in the kit body 1 to detect the chlorogenic acid content in samples such as traditional Chinese medicine, medicinal herbs, health products, and cosmetics, and it is difficult to observe the remaining amount of each chlorogenic acid content detection reagent or whether the chlorogenic acid content detection reagent is turned on, leading to difficulties for staff. To prevent situations where staff members take out insufficient chlorogenic acid content detection reagents at one time or unintentionally open new chlorogenic acid content detection reagents, resulting in staff needing to take out multiple chlorogenic acid content detection reagents or wasting reagents, the device's practicality is improved. The inner surface of the reagent kit body 1 is equipped with a protective device 3. By setting the protective device 3, the lower surface and four corners of the reagent kit body 1 are supported and protected, preventing the reagent kit body 1 from being squeezed by external forces or dropped on the ground during transportation, which could cause deformation of the reagent kit body 1 and damage to the reagents inside, thus improving the safety of the device.

[0034] The following section will explain the specific design and function of its lifting device 2 and protective device 3.

[0035] Reference Figure 2 , Figure 3 and Figure 4As shown, in this embodiment: the lifting device 2 includes a ring-shaped frame 21, which is slidably connected to the inner surface of the reagent kit body 1. Five chlorogenic acid standard solutions 4 of different concentrations are slidably inserted into the ring-shaped frame 21. An enzyme label 5 is slidably inserted into the ring-shaped frame 21. An anti-chlorogenic acid monoclonal antibody solution 6 is slidably inserted into the ring-shaped frame 21. A colorimetric reagent 7 is slidably inserted into the ring-shaped frame 21. A stop solution 8 is slidably inserted into the ring-shaped frame 21. A sliding block 22 is fixedly connected to one side of the ring-shaped frame 21. A sliding groove is opened on one side of the reagent kit body 1. The sliding block 22 is slidably connected to the inner surface of the sliding groove of the reagent kit body 1. A push-pull plate 23 is fixedly connected to the side of the sliding block 22 away from the ring-shaped frame 21. The side of plate 23 closest to sliding block 22 is slidably connected to reagent kit body 1. Several stabilizing rings 24 are fixedly connected to the inner surface of the ring-shaped frame 21. An enzyme-labeled plate 9 coated with chlorogenic acid A antigen or goat anti-mouse secondary antibody is adhered to one side of reagent kit body 1. A fixing rope 25 is fixedly connected to the upper surface of the push-pull plate 23, and a hook 26 is fixedly connected to one side of reagent kit body 1. When the staff pulls the ring-shaped frame 21 out of reagent kit body 1 with the reagent, they pull the fixing rope 25, which hangs on the inner surface of the hook 26. This achieves the effect of fixing the push-pull plate 23, thereby achieving the effect of fixing the ring-shaped frame 21, so that the staff can free up their hands to remove the reagent from the ring-shaped frame 21. The device is easy to remove, thus improving its convenience. A first spring 27 is fixedly connected to the side of the push-pull plate 23 away from the fixing rope 25. A semi-circular plate 28 is fixedly connected to the end of the first spring 27 away from the push-pull plate 23. The semi-circular plate 28 is fixedly connected to one side of the reagent kit body 1. When the staff needs to put the retractable frame 21 back into the reagent kit body 1, they pull the fixing rope 25 to disengage it from the hook 26. At this time, the rebound force of the first spring 27 causes the push-pull plate 23 to slide on one side of the reagent kit body 1. The push-pull plate 23 causes the sliding block 22 to slide in the sliding groove of the reagent kit body 1. At the same time, the sliding block 22 causes the retractable frame 21 to move downward until the retractable frame 21 is flush with the bottom of the inner surface of the reagent kit body 1. When the reagents are fully engaged, the first spring 27 automatically resets the reagents on the rack 21, reducing the number of steps required for operation and improving work efficiency. A soft pad 29 is fixedly connected to the inner surface of the stabilizing ring 24, and the size of the soft pad 29 is compatible with the size of the stabilizing ring 24. When the operator releases the limiting force of the fixing rope 25 on the rack 21, the rebound force of the first spring 27 resets the rack 21. The stabilizing ring 24 inside the rack 21 collides with the reagents placed on the rack 21. At this time, the soft pad 29 reduces the collision between the stabilizing ring 24 and the reagents on the rack 21, thereby improving the service life of the reagent kit body 1 and the reagents on the rack 21.

[0036] Reference Figure 2 , Figure 4 , Figure 5 and Figure 6 As shown, specifically, the protective device 3 includes four L-shaped plates 31, all of which are fixedly connected to the inner surface of the reagent kit body 1. Four protective blocks 32 are slidably inserted into the reagent kit body 1, with their surfaces slidably connected to the four L-shaped plates 31 respectively. A protective plate 33 is fixedly connected to one side of each of the four protective blocks 32, and a winding rope 34 is fixedly connected to both sides of the protective plate 33. A winding rod 35 is fixedly connected to both sides of the reagent kit body 1, with the arc surface of the winding rope 34 slidably connected to the winding rod 35. The four L-shaped plates 31 are arranged in pairs, with two support rods 36 fixedly connected to the side of each pair of L-shaped plates 31 closest to each other. When the reagent kit body 1 is dropped onto a sharp corner, the support rods 36 provide support to the reagent kit body. The internal structure of reagent kit 1 provides protection, preventing damage to the reagents inside when the kit body 1 is bumped against sharp corners (where the corner size is smaller than the kit body size), thus rendering the kit unusable. Four rubber stoppers 37 are fixedly connected to one side of reagent kit body 1. The size of the rubber stoppers 37 is adapted to the size of the L-shaped plate 31. When the staff needs to close reagent kit body 1, the rubber stoppers 37 on reagent kit body 1 can be inserted into the space created by the L-shaped plate 31 and the inner surface of reagent kit body 1. At this time, the rubber stoppers 37 achieve the effect of enhancing the stability of reagent kit body 1 when closed, preventing reagents inside reagent kit body 1 from falling out due to automatic opening.

[0037] Working principle: When staff need to use the reagents in reagent kit 1 to test the chlorogenic acid content in samples such as traditional Chinese medicine, medicinal herbs, health products, and cosmetics, the chlorogenic acid content detection reagent needs to be removed from reagent kit 1. At this time, the staff opens reagent kit 1 and pulls the fixing rope 25 upwards. The fixing rope 25 drives the push-pull plate 23 to move, stretching the first spring 27. The push-pull plate 23 drives the sliding block 22 to slide upwards within the sliding groove of reagent kit 1. Simultaneously, the sliding block 22 drives the ring frame 21 to slide within reagent kit 1, carrying the reagent placed on it upwards until the staff can clearly observe the chlorogenic acid content detection reagent. At this point, the fixing rope 25 is hung on the hook 26, and the staff can then remove the reagent from the ring frame 21. The device allows for the convenient use of chlorogenic acid content detection reagents. This facilitates the staff in fully extending the reagents within the kit body 1, making observation and selection easier. It avoids the difficulties staff face when testing chlorogenic acid content in samples such as traditional Chinese medicine, medicinal herbs, health products, and cosmetics. Previously, staff struggled to observe the remaining amount of each reagent or whether it was activated, leading to insufficient reagent usage or unintentional activation of new reagents, resulting in multiple reagent withdrawals or wasted reagents. This significantly improves the device's practicality.

[0038] When staff need to protect the reagent kit body 1 and its internal reagents, they push the protective plate 33, which in turn moves the protective block 32, causing it to slide on the inner surface of the L-shaped plate 31 until the protective plate 33 is in contact with the lower surface of the reagent kit body 1. At this point, they pull the winding rope 34, which wraps around the arc surface of the winding rod 35. Simultaneously, they press the reagent kit body 1, causing the rubber stopper 37 to be inserted into the space created by the L-shaped plate 31 and the inner surface of the reagent kit body 1. This achieves the effect of supporting and protecting the lower surface and four corners of the reagent kit body 1, preventing the reagent kit body 1 from being squeezed by external forces or dropped on the ground during transportation, which could cause deformation of the reagent kit body 1 and damage to the reagents inside, thus improving the safety of the device.

[0039] By preparing a monoclonal antibody solution specifically targeting small molecule compounds of traditional Chinese medicine (TCM) against chlorogenic acid, a simple, rapid, and sensitive immunoassay method for TCM can be established. Among the immunoassay methods, ELISA is the most commonly used. ELISA has the characteristics of high specificity, simple operation, high sensitivity, and easy automation. It can be used not only for the determination of monoclonal antibody solutions against chlorogenic acid, but also for the determination of antigens in samples. Its basic principle is as follows: (1) The antigen or monoclonal antibody solution against chlorogenic acid is physically adsorbed on the surface of the solid-phase carrier (ELISA plate) and maintains its immunological activity; (2) The antigen or monoclonal antibody solution against chlorogenic acid binds to the enzyme to form an enzyme conjugate and maintains immunological and enzyme activity; (3) The enzyme conjugate binds to the corresponding antigen or monoclonal antibody solution against chlorogenic acid. When a substrate is added, a color reaction occurs. Since the intensity of the color of the reactant is directly proportional to the amount of the corresponding antigen or monoclonal antibody solution against chlorogenic acid in the sample, the test results can be displayed according to the degree of substrate color development.

[0040] The detection method is as follows:

[0041] (1) Preparation of cleaning solution: Dilute the 10-fold concentrated cleaning solution with deionized water at a volume ratio of 1:9 according to the required amount (e.g., take 10 mL of 10-fold concentrated cleaning solution into 90 mL of distilled water) and mix well before use.

[0042] (2) Take out the required number of enzyme-labeled strips and insert them into the enzyme-labeled plate holder. Record the position of each standard and sample solution. To reduce the fluctuation of the detection value, perform a two-well parallel experiment.

[0043] (3) Add 50 μL of standard solution / sample solution to the corresponding microwell, then add 50 μL of enzyme label / well, 50 μL of antichlorogenic acid monoclonal antibody solution working solution / well, gently shake for 5 seconds to mix, cover the plate and place in a 25°C dark environment for 15 min to react.

[0044] (4) Remove the cover film, shake off the liquid in the hole, add 300μL of cleaning solution per hole, wash 4 times, pour out the cleaning solution, and pat dry with absorbent paper.

[0045] (5) Add 100 μL of substrate colorimetric solution per well, mix well, cover the plate and incubate in a dark environment at 25°C for 15 min.

[0046] (6) Add 100 μL of stop solution per well, set the microplate reader to 450 nm, and measure the OD value.

[0047] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

Claims

1. A detection kit for detecting chlorogenic acid content in a sample, comprising a kit body (1), characterized in that: The inner surface of the reagent kit body (1) is provided with a lifting device (2), the lifting device (2) includes a ring (21), the ring (21) is slidably connected to the inner surface of the reagent kit body (1), five chlorogenic acid standard solutions (4) of different concentrations are slidably inserted in the ring (21), an enzyme label (5) is slidably inserted in the ring (21), an anti-chlorogenic acid monoclonal antibody solution (6) is slidably inserted in the ring (21), a chromogenic agent (7) is slidably inserted in the ring (21), and a stop solution (8) is slidably inserted in the ring (21). A sliding block (22) is fixedly connected to one side of the kit body (1). A sliding groove is provided on one side of the kit body (1). The sliding block (22) is slidably connected to the inner surface of the sliding groove of the kit body (1). A push-pull plate (23) is fixedly connected to the side of the sliding block (22) away from the ring frame (21). The push-pull plate (23) is slidably connected to the kit body (1) on the side close to the sliding block (22). Several stabilizing rings (24) are fixedly connected to the inner surface of the ring frame (21). An enzyme-labeled plate (9) coated with chlorogenic acid A antigen or goat anti-mouse secondary antibody is adhered to one side of the kit body (1).

2. The detection kit for detecting chlorogenic acid content in a sample according to claim 1, characterized in that: A fixing rope (25) is fixedly connected to the upper surface of the push-pull plate (23), and a hook (26) is fixedly connected to one side of the reagent kit body (1).

3. The detection kit for detecting chlorogenic acid content in a sample according to claim 2, characterized in that: A first spring (27) is fixedly connected to the side of the push-pull plate (23) away from the fixed rope (25). A semi-circular plate (28) is fixedly connected to the end of the first spring (27) away from the push-pull plate (23). The semi-circular plate (28) is fixedly connected to one side of the reagent kit body (1).

4. The detection kit for detecting chlorogenic acid content in a sample according to claim 1, characterized in that: A soft pad (29) is fixedly connected to the inner surface of the stabilizing ring (24), and the size of the soft pad (29) is adapted to the size of the stabilizing ring (24).

5. The detection kit for detecting chlorogenic acid content in a sample according to claim 1, characterized in that: The inner surface of the reagent kit body (1) is provided with a protective device (3). The protective device (3) includes four L-shaped plates (31). The four L-shaped plates (31) are fixedly connected to the inner surface of the reagent kit body (1). Four protective blocks (32) are slidably inserted into the reagent kit body (1). The surfaces of the four protective blocks (32) are slidably connected to the four L-shaped plates (31) respectively. A protective plate (33) is fixedly connected to one side of the four protective blocks (32). A winding rope (34) is fixedly connected to both sides of the protective plate (33). A winding rod (35) is fixedly connected to both sides of the reagent kit body (1). The arc surface of the winding rope (34) is slidably connected to the winding rod (35).

6. The detection kit for detecting chlorogenic acid content in a sample according to claim 5, characterized in that: The four L-shaped plates (31) are arranged in pairs, and two support rods (36) are fixedly connected to the side of each pair of L-shaped plates (31) that are close to each other.

7. The detection kit for detecting chlorogenic acid content in a sample according to claim 5, characterized in that: Four rubber stoppers (37) are fixedly connected to one side of the reagent kit body (1), and the size of the rubber stoppers (37) is adapted to the size of the L-shaped plate (31).

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