Liquid cover film

Abstract

The utility model relates to a liquid cover film which comprises a tail plate, a cover plate and a connecting plate, the tail plate and the connecting plate are respectively arranged at two ends of the cover plate, a reaction cavity is formed between the cover plate and a glass slide, and the liquid cover film further comprises a drainage plate arranged in the middle of the tail plate; the first end face is arranged on the lower end face of the tail plate and connected with the end, facing the cover plate, of the drainage plate. The second end face is adjacent to the first end face; the liquid homogenizing bulges are arranged on the second end surface, and the number of the liquid homogenizing bulges is multiple; the multiple drainage grooves are formed in the first end face and formed in the two sides of the drainage plate. Due to the fact that the drainage groove is formed in the first end face of the tail plate, reaction liquid can rapidly flow into the reaction cavity along the drainage groove in the reaction liquid adding stage, and rapid filling of the reaction cavity is promoted; and the second end face is also provided with a liquid homogenizing bulge, so that the liquid homogenizing bulge can uniformly distribute the reaction liquid on the glass slide when the liquid cover film is dragged by the full-automatic immunohistochemical instrument, and the uniform mixing of the reaction liquid is accelerated.

Description

Technical Field

[0001] This utility model relates to the field of biological sample slide processing technology, and in particular to a liquid capping membrane. Background Technology

[0002] During the processing of biological sample slides, a reaction solution needs to be added to the reaction chamber between the liquid cover membrane and the glass slide, so that the biological sample and the reaction solution can fully react in the reaction chamber, and the antigenic peptides and proteins in the biological sample tissue sections can be quantitatively analyzed.

[0003] Current technologies often place the liquid capping film at a certain angle to the plane of the glass slide, then add the reaction liquid to one end. Gravity forces the reaction liquid into the reaction chamber, expelling any gas and filling the chamber completely. Chinese Patent CN111562164A discloses a liquid capping film with a drainage column at the front center to guide the reaction liquid to a liquid distribution section. When adding the reaction liquid, it is added to the drainage column, ensuring the liquid reaches the middle of the horizontal position on the glass slide. Due to the special structure of the cone, the liquid diffuses evenly from the center downwards, quickly filling the liquid distribution section. Then, the reaction liquid spreads evenly and rapidly throughout the reaction chamber, uniformly covering the biological sample and promoting a thorough reaction between the sample and the reaction liquid. However, the reaction solution can only flow into the reaction chamber along the drainage column. This means that the amount of reaction solution added instantaneously cannot be too large; it must be ensured that the reaction solution flows only downwards along the drainage column. This increases the time required to add the reaction solution, and it does not reduce the time required for the reaction solution to diffuse evenly into the reaction chamber. The reaction solution flowing into the reaction chamber still diffuses within the chamber by gravity. Furthermore, excessive stirring of the reaction solution in the reaction chamber can easily damage the tissue sections within it.

[0004] Therefore, this utility model provides a liquid capping membrane. Utility Model Content

[0005] In view of the above-mentioned shortcomings and deficiencies of the prior art, the present invention provides a liquid cap membrane, thereby solving the technical problem of how to make the reaction liquid in the reaction chamber spread rapidly and uniformly.

[0006] To achieve the above objectives, this utility model provides a liquid cover membrane, including a tail plate, a cover plate, and a connecting plate, wherein the tail plate and the connecting plate are respectively disposed at both ends of the cover plate, and a reaction chamber is formed between the cover plate and the glass slide. The membrane also includes: a flow guide plate disposed in the middle of the tail plate; a first end face disposed on the lower end face of the tail plate and connected to the end of the flow guide plate facing the cover plate; a second end face disposed adjacent to the first end face; multiple flow uniform protrusions disposed on the second end face; and multiple flow channels formed on the first end face and disposed on both sides of the flow guide plate.

[0007] Optionally, the number of uniform liquid protrusions is even, and the even number of uniform liquid protrusions are symmetrically arranged on both sides of the drainage plate.

[0008] Optionally, an even number of uniformly spaced liquid-distributing protrusions are arranged on the second end face.

[0009] Optionally, support columns are installed on the tail plate, and there are two support columns, which are symmetrically arranged on both sides of the diversion plate; in the horizontal direction, the length of the support column is greater than that of the tail plate.

[0010] Optionally, there are two diversion channels, which are symmetrically arranged on both sides of the diversion plate.

[0011] Optionally, the opening of the diversion channel gradually increases from top to bottom along the extension direction of the channel.

[0012] Optionally, the direction of the drainage channel is to extend gradually away from the drainage plate.

[0013] The height of the uniform liquid protrusion can be selected as 0.05mm-0.1mm.

[0014] The beneficial effects of this utility model are:

[0015] This invention provides a liquid cap membrane. Because multiple drainage grooves are formed on the first end face of the tail plate, during the addition of reaction liquid to the reaction chamber, the reaction liquid can not only flow downwards along the drainage plate but also rapidly diffuse into the reaction chamber along the drainage grooves. Since the drainage grooves are located on both sides of the drainage plate, the distance between the reaction liquid flowing down the drainage grooves and the edge of the reaction chamber is shorter, facilitating the rapid filling of the reaction chamber in the width direction. Furthermore, by setting multiple uniform liquid protrusions on the second end face of the tail plate, the liquid cap membrane is moved by the fully automated immunohistochemistry instrument... During the process, multiple uniform liquid protrusions provide the reaction liquid with a force to diffuse around the reaction chamber. On the one hand, as the liquid cover film is dragged, the multiple uniform liquid protrusions accelerate the diffusion of the reaction liquid along the length of the reaction chamber. On the other hand, in the width direction of the reaction chamber, the multiple uniform liquid protrusions also occupy a certain volume of the reaction liquid during the filling of the reaction liquid, further promoting the diffusion of the reaction liquid in the width direction of the reaction chamber. In summary, by setting multiple uniform liquid protrusions, the diffusion speed of the reaction liquid is accelerated, so that the reaction liquid in the reaction chamber is quickly and evenly distributed on the glass slide, and the mixing of the reaction liquid is accelerated. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the liquid cap membrane of this utility model;

[0017] Figure 2 In order to be in Figure 1 Enlarged diagram of point A in the middle.

[0018] Explanation of reference numerals in the attached figures:

[0019] 1. Tail plate; 2. Cover plate; 3. Connecting plate; 4. First end face; 5. Second end face; 6. Uniform liquid protrusion; 7. Drainage plate; 8. Drainage groove; 9. Reaction chamber; 10. Support column. Detailed Implementation

[0020] To better understand the above technical solutions, exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although exemplary embodiments of the present invention are shown in the drawings, it should be understood that the present invention can be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that the present invention can be understood more clearly and thoroughly, and that the scope of the present invention can be fully conveyed to those skilled in the art.

[0021] To facilitate demonstration of the structure of the reaction chamber 9 side in the liquid cap membrane of this invention, this invention... Figure 1 and Figure 2 These are all schematic diagrams showing the reaction chamber 9 with the marked position facing upwards. In actual use, the liquid cover membrane... Figure 1 The position of reaction chamber 9 marked in the middle is downward.

[0022] An embodiment of this utility model provides a liquid capping membrane, such as... Figure 1 and Figure 2 As shown, the device includes a tail plate 1, a cover plate 2, and a connecting plate 3. The tail plate 1 and the connecting plate 3 are respectively disposed at both ends of the cover plate 2. A reaction chamber 9 is formed between the cover plate 2 and the glass slide. The device also includes: a flow guide plate 7 disposed in the middle of the tail plate 1; a first end face 4 disposed on the lower end face of the tail plate 1 and connected to the end of the flow guide plate 7 facing the cover plate 2; a second end face 5 disposed adjacent to the first end face 4; a liquid uniforming protrusion 6 disposed on the second end face 5, and there are multiple liquid uniforming protrusions 6; and a flow guide groove 8 formed on the first end face 4 and disposed on both sides of the flow guide plate 7, and there are multiple flow guide grooves 8.

[0023] For example, the homogenizing protrusion 6 and the tail plate 1 are integrally formed and connected, and the shape of the homogenizing protrusion 6 facing the glass slide is hemispherical, thereby ensuring that when the homogenizing protrusion 6 diffuses the reaction liquid in the reaction chamber 9, the smooth surface of the hemispherical protrusion faces the glass slide, avoiding damage to the tissue section in the reaction liquid by the homogenizing protrusion 6 during the homogenization process.

[0024] For example, the guide plate 7 has a conical structure, which facilitates the rapid flow of the reaction liquid along the cone towards the glass slide. Since the guide plate 7 is positioned in the middle of the tail plate 1, it ensures that most of the reaction liquid flows into the reaction chamber 9 at the middle of the width of the reaction chamber 9, facilitating the diffusion of the reaction liquid from the middle of the glass slide to the surrounding area. Because there is a distance between the middle position and the side of the reaction chamber 9, by providing guide grooves 8 on both sides of the guide plate 7, excess reaction liquid can flow along the guide grooves 8 towards the reaction chamber 9 during the addition of reaction liquid. This avoids all the reaction liquid flowing only along the conical guide plate 7, accelerating the flow rate of the reaction liquid towards the side of the reaction chamber 9, shortening the time it takes for the reaction liquid to fill the reaction chamber 9, and further improving the efficiency of filling the reaction chamber 9.

[0025] In one possible embodiment, such as Figure 1 and Figure 2 As shown, the number of uniform liquid protrusions 6 is even, and the even number of uniform liquid protrusions 6 are symmetrically arranged on both sides of the diversion plate 7.

[0026] For example, in this embodiment, the number of uniform liquid protrusions 6 is 4, such as... Figure 2 As shown, since the drainage plate 7 is located in the middle of the tail plate 1, correspondingly, two homogenizing protrusions 6 are located on the left side of the drainage plate 7, and the other two homogenizing protrusions 6 are located on the right side of the drainage plate 7. The connecting plate 3 at the end of the liquid cover film is connected to the power end of the fully automated immunohistochemistry instrument. In this way, when the liquid cover film is dragged by the fully automated immunohistochemistry instrument, the four homogenizing protrusions 6 provide a force for the reaction liquid to diffuse around the reaction chamber 9. That is, two homogenizing protrusions 6 accelerate the flow speed of the reaction liquid near the left side of the reaction chamber 9, and the other two homogenizing protrusions 6 accelerate the flow speed of the reaction liquid near the right side of the reaction chamber 9. Thus, the reaction liquid in the reaction chamber 9 is uniformly and quickly distributed on the glass slide, which accelerates the mixing of the reaction liquid.

[0027] In one possible embodiment, such as Figure 1 and Figure 2 As shown, an even number of uniform liquid protrusions 6 are evenly spaced on the second end face 5.

[0028] For example, in this embodiment, there are two uniform liquid protrusions 6. The two uniform liquid protrusions 6 are respectively facing the two diversion channels 8. That is, the uniform liquid protrusions 6 located on the same side of the diversion plate 7 correspond to the positions of the diversion channels 8. This arrangement allows the reaction liquid flowing into the diversion channels 8 to diffuse more quickly through the corresponding uniform liquid protrusions 6, further accelerating the diffusion and mixing speed of the reaction liquid.

[0029] For example, in this embodiment, there are four uniform liquid protrusions 6. The four uniform liquid protrusions 6 are respectively positioned opposite the edges of the two flow channels 8. Each flow channel 8 has two edges in the direction of reaction liquid flow. By aligning the positions of the two uniform liquid protrusions 6 on the same side with the two edges of the flow channel 8, the reaction liquid is more likely to flow along the edges of the flow channel 8. This arrangement allows the reaction liquid flowing into the flow channel 8 to diffuse more quickly through the corresponding uniform liquid protrusions 6, further accelerating the diffusion and mixing speed of the reaction liquid.

[0030] For example, in this embodiment, there are 6 uniform liquid protrusions 6. In the width direction of the reaction chamber 9, during the uniform liquid process, the 6 uniform liquid protrusions 6 can occupy a larger volume of the reaction liquid. This arrangement allows the reaction liquid flowing in from the drainage channel 8 to diffuse more quickly in the width direction of the reaction chamber 9 through the action of the uniform liquid protrusions 6, thereby further accelerating the diffusion and mixing speed of the reaction liquid.

[0031] In one possible embodiment, such as Figure 1 and Figure 2 As shown, there are two support columns 10, which are symmetrically arranged on both sides of the diversion plate 7. In the horizontal direction, the length of the support column 10 is greater than that of the tail plate 1.

[0032] For example, before adding the reaction liquid to the reaction chamber 9, the connecting plate 3 of the liquid cover membrane is raised to a certain height, so that the second end face 5 gradually approaches or touches the glass slide. At this time, the connecting plate 3 is raised further, and the liquid cover membrane as a whole contacts the glass slide with the two support columns 10 as support points. At this time, the tail plate 1 is in the middle position of the glass slide, and then the reaction liquid is added. The addition position is mainly the guide plate 7. Since the guide groove 8 is close to the guide plate 7, some of the reaction liquid flows towards the glass slide in a diffused manner along the guide groove 8, thereby accelerating the speed of uniform diffusion of the reaction liquid and accelerating the mixing of the reaction liquid.

[0033] For example, the first end face 4 and the second end face 5 are both elongated end faces disposed between the two support columns 10, that is, the first end face 4 and the second end face 5 are both end faces disposed along the width direction of the reaction chamber 9. The first end face 4 is in contact with the guide plate 7. Therefore, a guide groove 8 is provided on the first end face 4 to facilitate the reaction liquid flowing down along the guide plate 7 to flow to the glass slide through the guide groove 8.

[0034] In one possible embodiment, such as Figure 1 and Figure 2 As shown, there are two diversion channels 8, which are symmetrically arranged on both sides of the diversion plate 7.

[0035] For example, if the two drainage channels 8 are symmetrically arranged, the reaction liquid flowing into the two drainage channels 8 will have the same degree of diffusion, which is beneficial to the uniform diffusion of the reaction liquid after entering the reaction chamber 9.

[0036] In one possible embodiment, such as Figure 1 and Figure 2 As shown, along the extension direction of the diversion channel 8, from top to bottom, the opening of the diversion channel 8 gradually increases.

[0037] For example, according to the actual placement direction of the liquid cap membrane, the opening of the upper part of the drainage channel 8 is relatively small, while the opening of the lower part is relatively large. This setting allows the reaction liquid flowing into the drainage channel 8 to diffuse into the reaction chamber 9, thereby increasing the diffusion rate of the reaction liquid in the reaction chamber 9.

[0038] In one possible embodiment, such as Figure 1 and Figure 2 As shown, the extension direction of the drainage groove 8 is towards the direction of gradually moving away from the drainage plate 7.

[0039] For example, the extension direction of the flow channel 8 is inclined from the position close to the flow plate 7 to the position away from the flow plate 7, that is, gradually away from the flow plate 7. This setting is conducive to the diffusion of the reaction liquid from the middle position of the reaction chamber 9 to the edges on both sides of the reaction chamber 9, thereby accelerating the diffusion speed of the reaction liquid in the reaction chamber 9.

[0040] In one possible embodiment, the height of the uniform liquid protrusion 6 in the direction toward the reaction chamber 9 is 0.05mm-0.1mm.

[0041] For example, the reaction chamber 9 serves as a cavity for containing the reaction liquid. In this embodiment, the height of the reaction chamber 9 itself is 2mm. Therefore, to ensure a seal, the height of the liquid-uniforming protrusion 6 in the vertical direction cannot be lower than the lower edge of the reaction chamber 9. The reaction chamber 9 is composed of a liquid cover film located above and a glass slide located below. Therefore, the four circumferential edges of the reaction chamber 9 are the three edges of the cover plate 2 and the edge of the tail plate 1, respectively. That is, the liquid-uniforming protrusion 6 in the vertical direction cannot be lower than the three edges of the cover plate 2, nor can it be lower than the edge of the tail plate 1, to ensure that the three edges of the cover plate 2 and the edge of the tail plate 1 can be completely fitted with the glass slide.

[0042] For example, in order to avoid the homogenizing protrusion 6 from touching the tissue section and causing damage to the tissue section, the height of the homogenizing protrusion 6 should not be too high. In this embodiment, the height of the homogenizing protrusion 6 is 0.05 mm. Correspondingly, the concentration of the added reaction solution is relatively small, the viscosity of the reaction solution is also relatively small, and the fluidity of the reaction solution is good. Therefore, the homogenizing protrusion 6 is set to 0.05 mm.

[0043] For example, the height of the uniform liquid protrusion 6 in this embodiment is 0.1 mm. Correspondingly, the concentration of the added reaction solution is relatively large, the viscosity of the reaction solution is also large, and the fluidity of the reaction solution is relatively poor. Therefore, the uniform liquid protrusion 6 is set to 0.1 mm to increase the uniform liquid protrusion 6 on the reaction solution, thereby accelerating the mixing of the reaction solution.

[0044] For example, the height of the uniform liquid protrusion 6 can also be 0.07 mm, and the specific height of the uniform liquid protrusion 6 depends on the actual situation.

[0045] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood according to the specific circumstances.

[0046] In this utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "beneath" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0047] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make modifications, alterations, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A liquid capping membrane, comprising a tail plate, a cover plate, and a connecting plate, wherein the tail plate and the connecting plate are respectively disposed at both ends of the cover plate, and a reaction chamber is formed between the cover plate and a glass slide, characterized in that, Also includes: A diversion plate is disposed in the middle of the tail plate; The first end face is located on the lower end face of the tail plate and is connected to the end of the diversion plate facing the cover plate. The second end face is disposed adjacent to the first end face; A liquid homogenizing protrusion is provided on the second end face, and the number of the liquid homogenizing protrusion is multiple; A flow channel is formed on the first end face and disposed on both sides of the flow channel plate, and there are multiple flow channels.

2. The liquid capping membrane as described in claim 1, characterized in that: The number of the liquid-dispersing protrusions is even, and the even number of liquid-dispersing protrusions are symmetrically arranged on both sides of the drainage plate.

3. The liquid capping membrane as described in claim 2, characterized in that, An even number of the uniform liquid protrusions are evenly spaced on the second end face.

4. The liquid capping membrane as described in claim 1, characterized in that, Also includes: Support columns are provided on the tail plate, and there are two support columns, which are symmetrically arranged on both sides of the diversion plate; In the horizontal direction, the length of the support column is greater than that of the tail plate.

5. The liquid cap membrane as described in claim 1, characterized in that, There are two drainage channels, which are symmetrically arranged on both sides of the drainage plate.

6. The liquid capping membrane as described in claim 5, characterized in that, Along the extension direction of the drainage channel, from top to bottom, the opening of the drainage channel gradually increases.

7. The liquid capping membrane as described in claim 6, characterized in that, The direction in which the drainage channel extends is towards gradually moving away from the drainage plate.

8. The liquid cap membrane according to any one of claims 1 to 7, characterized in that, The height of the uniform liquid protrusion is 0.05mm-0.1mm.

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