Light shielding box for reaction reagent
By designing a light-proof reaction reagent box, the problem of easy contamination of the colorimetric solution was solved, enabling efficient and safe ELISA experimental operations and improving the flexibility and convenience of the laboratory.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- GUANGZHOU JINYILI PHARM TECH CO LTD
- Filing Date
- 2025-03-31
- Publication Date
- 2026-05-08
AI Technical Summary
The current ELISA experiments lack sufficient protection of the chromogenic solution, making the experimental results susceptible to interference from contaminants, resulting in low operational efficiency and poor flexibility.
A light-proof reagent box was designed, including a light-proof box body and a light-proof cover, which are sealed by a locking assembly. It has a material dispensing hole and is made of transparent brown and black plastic. Together with the light-proof cylinder, it is used to store the colorimetric solution in the dark and facilitates the operation of the sample dispenser.
It effectively protects the colorimetric solution from contamination, improves the efficiency and safety of experimental operations, and enhances the flexibility and convenience of operation.
Smart Images
Figure CN224211554U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of molecular biology equipment technology, and in particular to a light-proof box for reaction reagents. Background Technology
[0002] Enzyme-linked immunosorbent assay (ELISA) is a widely used qualitative and quantitative detection method in the field of immunology, detecting antigens through the specific binding reaction of antibodies. One of the key steps in an ELISA experiment is adding the chromogenic reagent to the microplate, where a colorimetric reaction generates a signal value proportional to the amount of analyte being detected. During this process, the chromogenic reagent and the colorimetric reaction must be protected from light to ensure the accuracy and reliability of the experimental results.
[0003] When performing multiple ELISA experiments, automated sample dispensers are often used for batch operations. To effectively protect the sample from light, laboratories typically employ the following methods: First, turning off the fluorescent lights and turning on red lights. This method partially reduces light interference but decreases experimental efficiency, and prolonged exposure to red light may negatively impact the eyesight of laboratory personnel. Second, placing the sample dispenser in a light-proof enclosure. While this effectively blocks light, the enclosure occupies considerable space and places high demands on the electrical layout of the lab bench, limiting the flexibility and convenience of the experiment. Third, storing the chromogenic solution in open-top containers facilitates the dispenser's aspiration and transfer to the microplate. However, this open container has significant drawbacks: the chromogenic solution is not effectively protected and is susceptible to interference from environmental contaminants; contaminant spillage can lead to inaccurate experimental results.
[0004] Therefore, existing technologies still have certain shortcomings in terms of protecting the colorimetric solution, improving the efficiency of experimental operations, and optimizing light-protection conditions. For this reason, a novel light-protection box for reaction reagents is urgently needed. Utility Model Content
[0005] The purpose of this invention is to provide a light-proof box for reaction reagents, which aims to solve the problems mentioned above. Specifically, it can effectively protect the colorimetric solution from contamination, improve the efficiency and safety of experimental operations, and enhance the flexibility and convenience of experimental operations.
[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows:
[0007] This application provides a light-proof container for reaction reagents, comprising:
[0008] The light-proof box has an opening at the top and an internal storage space for storing reagents in the dark.
[0009] The light-shielding cover has a first end hinged to the opening of the light-shielding box, and a second end provided with a locking component for locking to the light-shielding box;
[0010] Several dispensing holes are located on the top of the light-proof cover, for pipette tips to pass through the light-proof box to extract reagents.
[0011] In one possible implementation, the locking assembly includes a buckle and a slot, the buckle is mounted on the second end of the light-shielding cover, the slot is disposed on the light-shielding box, and the buckle is detachably snapped into the slot;
[0012] Alternatively, the locking assembly includes a first latch and a second latch, with the first latch installed on the second end of the light-shielding cover and the second latch disposed on the light-shielding box, and the first latch and the second latch being detachably locked together.
[0013] In one possible implementation, the light-shielding box is made of transparent brown plastic.
[0014] In one possible implementation, liquid markings are provided on the outer side of the light-shielding box.
[0015] In one possible implementation, the light-shielding cover is made of black, opaque plastic.
[0016] In one possible implementation, the bottom side of the light-shielding box is provided with locking feet.
[0017] In one possible implementation, a handle is fixedly mounted on the light-shielding cover.
[0018] In one possible implementation, the first end of the light-shielding cover is hinged to the opening of the light-shielding box via a hinge.
[0019] In one possible implementation, each of the feeding holes has a vertically downward light-blocking cylinder at its bottom, the size of which is adapted to the pipette tip to reduce the amount of light source entering the light-blocking box along the upper side of the light-blocking cover.
[0020] In one possible implementation, the number of feed holes is several and they are arranged in a matrix-like spacing.
[0021] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are:
[0022] In this invention, the combination of the light-shielding box and the light-shielding cover can effectively protect the color-developing liquid inside the light-shielding box from light, preventing the color-developing liquid from being interfered with by environmental pollutants and preventing pollutants from falling and causing deviations in experimental results. At the same time, this design improves the efficiency and safety of experimental operations. The light-shielding box is small in size and occupies little space, further enhancing the flexibility and convenience of experimental operations. Attached Figure Description
[0023] Figure 1 This is a schematic diagram of the structure of this utility model;
[0024] Figure 2 This is a schematic diagram of the structure of the light-blocking cylinder 7 of this utility model.
[0025] Marked in the image:
[0026] 1. Light-proof box body; 2. Clamping feet; 3. Light-proof cover body; 4. Handle; 5. Liquid scale; 6. Dispensing port; 7. Light-proof cylinder body; 8. Pipette tip; 9. Light source. Detailed Implementation
[0027] To make the objectives, technical solutions, and advantages of this application clearer, the embodiments of this application will be described in further detail below with reference to the accompanying drawings.
[0028] In related technologies, automated sample dispensers are often used for batch operations when performing multiple ELISA experiments. To achieve effective light protection during this process, laboratories typically employ the following methods: First, turning off the fluorescent lights and turning on red lights. This method can partially reduce light interference, but it also reduces experimental efficiency, and prolonged exposure to red light may negatively impact the eyesight of laboratory personnel. Second, placing the sample dispenser in a light-proof enclosure. While this effectively blocks light, the enclosure occupies considerable space and places high demands on the electrical layout of the laboratory table, limiting the flexibility and convenience of the experimental operation. Third, the chromogenic solution is usually stored in an open box container for easy aspiration and transfer to the microplate by the sample dispenser; however, this open container has significant drawbacks: the chromogenic solution is not effectively protected and is susceptible to interference from environmental contaminants, which can lead to deviations in experimental results.
[0029] To address the aforementioned problems, a light-proof container for the reaction reagents is proposed. (Using...) Figure 1 The overall structure of the light-proof box for the reaction reagents will be described. Figure 1 This is a schematic diagram of the structure of this utility model.
[0030] like Figure 1 As shown, the light-proof reagent box includes a light-proof box body 1, a light-proof cover 3, and a dispensing port 6, wherein:
[0031] The light-proof box 1 is made of transparent brown plastic. The top of the box 1 has an opening, and the interior has a hollow structure; therefore, the light-proof box 1 has a storage space for storing reagents in the dark. During ELISA experiments, the chromogenic solution (reagent) can be contained in a container and then placed in the storage space of the light-proof box 1, thus protecting the chromogenic solution from light and facilitating subsequent aspiration and transfer by the pipette tip 8 on the pipette.
[0032] The light-shielding cover 3 is made of black opaque plastic. The first end of the light-shielding cover 3 is hinged to the opening of the light-shielding box 1. Specifically, the first end of the light-shielding cover 3 can be hinged to the opening of the light-shielding box 1. A locking component is provided on the second end of the light-shielding cover 3 to lock it onto the light-shielding box 1. When the locking component is in the unlocked state, the light-shielding cover 3 can be hinged and rotated, allowing the top opening of the light-shielding box 1 to open. At this time, a container holding the color developing solution can be passed through the top opening of the light-shielding box 1 and placed inside the light-shielding box 1. Then, the light-shielding cover 3 is closed, and the locking component is set to the locked state.
[0033] There are several material extraction holes 6, which are located on the top of the light-proof cover 3. Specifically, there are 96 material extraction holes 6, which are arranged in a matrix. The material extraction holes 6 are used for pipette tips 8 to pass through the light-proof box 1 to extract reagents.
[0034] In this embodiment, through the cooperation of the light-shielding box 1 and the light-shielding cover 3, when the pipette is to be used for sample addition that requires light protection, the light-shielding box 1 is placed on the movable working plate position, and then the light-shielding cover 3 is opened to add the reagent that needs to be protected from light, i.e., the colorimetric solution, into the light-shielding box 1; then the light-shielding cover 3 is closed, and the light-shielding cover 3 can be fixedly installed on the light-shielding box 1 by the locking assembly; after the pipette is started, the pipette inserts the 96 matching pipette tips 8 into the light-shielding box 1 through the 96 dispensing holes 6 on the light-shielding cover 3 to draw up the reagent, thereby completing the pipetting operation;
[0035] Compared to existing light-shielding methods, the combination of the light-shielding box 1 and the light-shielding cover 3 can protect the colorimetric solution inside the light-shielding box 1 from light, preventing interference from environmental pollutants and avoiding deviations in experimental results caused by pollutants falling into the colorimetric solution. Compared to the light-shielding method of turning off the fluorescent lights and turning on the red lights in the laboratory, it can improve the efficiency and safety of experimental operations. Compared to the light-shielding method of placing the sample dispenser in the light-shielding box, the light-shielding box 1 is smaller in size and occupies less space, thereby improving the flexibility and convenience of experimental operations.
[0036] In some embodiments, such as Figure 1 As shown. Figure 1 This is a schematic diagram of the structure of this utility model. The locking assembly includes a buckle and a slot (not shown in the figure). The buckle is installed on the second end of the light-shielding cover 3, and the slot is provided on the light-shielding box 1. The buckle can be detachably engaged in the slot. When the buckle is engaged in the slot, the light-shielding cover 3 can be fixedly installed on the opening of the light-shielding box 1. When the buckle is removed from the slot, the light-shielding cover 3 can be opened from the opening of the light-shielding box 1.
[0037] Alternatively, the locking assembly includes a first latch and a second latch (not shown in the figure). The first latch is installed on the second end of the light-shielding cover 3, and the second latch is located on the light-shielding box 1. The first latch and the second latch are detachably locked together. When the first latch and the second latch are locked together, the light-shielding cover 3 can be fixedly installed on the opening of the light-shielding box 1. When the first latch and the second latch are detached, the light-shielding cover 3 can be opened from the opening of the light-shielding box 1. In this embodiment, the locking assembly has a simple structure, is easy to assemble and disassemble, and facilitates the detachable installation of the light-shielding cover 3 onto the opening of the light-shielding box 1.
[0038] In some embodiments, such as Figure 1 As shown. Figure 1 This is a schematic diagram of the structure of this utility model. As described above, the light-shielding box 1 is made of transparent brown plastic, and a liquid scale 5 can be provided on the outer side of the light-shielding box 1. In this embodiment, because the light-shielding box 1 is made of transparent brown plastic, the color developing liquid inside the light-shielding box 1 can be observed, and the remaining amount of color developing liquid can be easily observed through the liquid scale 5.
[0039] In some embodiments, such as Figure 1 As shown. Figure 1 This is a schematic diagram of the structure of this utility model. In existing light-shielding boxes 1, when placed on a movable working plate, the working plate and the container are not in close contact, causing the light-shielding box 1 to slide relative to each other, resulting in spillage of the developing solution. To solve this technical problem, a retaining foot 2 can be provided on the bottom side of the light-shielding box 1. The retaining foot 2 has a certain degree of elasticity and can be made of plastic. When using the light-shielding box 1, the first step is to open the retaining foot 2, creating a recessed groove on the surface of the movable working plate. The retaining foot 2 can abut against the groove wall, thus ensuring the light-shielding box 1 is tightly positioned and installed in the working plate. The second step is to close the light-shielding cover 3 and then lock the locking component on the second end of the light-shielding cover 3. These first and second steps effectively prevent reagent spillage.
[0040] In some embodiments, such as Figure 1 As shown,Figure 1 This is a schematic diagram of the structure of the present invention. In order to facilitate the opening of the light-shielding cover 3 under force, a handle 4 is fixedly installed on the light-shielding cover 3. Specifically, there is at least one handle 4, and the handle 4 is fixed to the side of the light-shielding cover 3.
[0041] In some embodiments, such as Figure 2 As shown. Figure 2 This is a schematic diagram of the structure of the light-blocking cylinder 7 in this utility model. Each material feeding hole 6 has a vertically downward light-blocking cylinder 7 at its bottom. The length of the light-blocking cylinder 7 is appropriate so that its light-blocking cover 3 can be opened reasonably along the top opening of the light-blocking box 1.
[0042] The size of the light-blocking cylinder 7 is adapted to the pipette tip 8. The light-blocking cylinder 7 can be used to reduce the light source 9 from entering the light-blocking box 1 along the upper side of the light-blocking cover 3, thereby ensuring that the light-blocking box 1 and the light-blocking cover 3 can effectively prevent the color developing liquid from being affected by light, and further improve the light-blocking effect.
[0043] In the description of this utility model, it should be noted that the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element.
[0044] Furthermore, in the description of this utility model, the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.
[0045] On the other hand, it should be noted that, unless otherwise explicitly specified and limited, the terms "located at," "installed," "connected," and "linked" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium; and they can refer to the internal connection of two components. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood according to the specific circumstances.
Claims
1. A light-proof container for reaction reagents, characterized in that, include: The light-proof box (1) has an opening at the top and an internal storage space for storing reagents in the dark. The light-shielding cover (3) has its first end hinged to the opening of the light-shielding box (1), and its second end is provided with a locking assembly for locking to the light-shielding box (1); Several material extraction holes (6) are provided on the top of the light-proof cover (3) for pipette tips (8) to pass through the light-proof box (1) to extract reagents.
2. The light-proof reagent box according to claim 1, characterized in that, The locking assembly includes a buckle and a slot. The buckle is installed on the second end of the light-shielding cover (3), and the slot is located on the light-shielding box (1). The buckle is detachably snapped into the slot. Alternatively, the locking assembly includes a first latch and a second latch, with the first latch installed on the second end of the light-shielding cover (3) and the second latch located on the light-shielding box (1), and the first latch and the second latch being detachably locked together.
3. The light-proof reagent box according to claim 1, characterized in that, The light-shielding box (1) is made of transparent brown plastic.
4. The light-proof reagent box according to claim 3, characterized in that, The light-proof box (1) has liquid scales (5) on its outer side.
5. The light-proof reagent box according to claim 1, characterized in that, The light-shielding cover (3) is made of black opaque plastic.
6. The light-proof reagent box according to claim 1, characterized in that, The bottom side of the light-shielding box (1) is provided with a locking foot (2).
7. The light-proof reagent box according to claim 1, characterized in that, A handle (4) is fixedly installed on the light-proof cover (3).
8. The light-proof reagent box according to claim 1, characterized in that, The first end of the light-shielding cover (3) is hinged to the opening of the light-shielding box (1) via a hinge.
9. The light-proof reagent box according to claim 1, characterized in that, Each of the feeding holes (6) has a vertically downward light-blocking cylinder (7) at its bottom. The size of the light-blocking cylinder (7) is adapted to the pipette tip (8) to reduce the light source (9) from entering the light-blocking box (1) along the upper side of the light-blocking cover (3).
10. The light-proof reagent box according to claim 1 or 9, characterized in that, The number of material feeding holes (6) is 96, and they are arranged in a matrix-like interval.