Large organism stress and culture device based on underwater vehicle wireless communication

Abstract

The utility model relates to a large-scale organism stress and culture device based on wireless communication of an underwater vehicle, which comprises a wireless communication subsystem, a control and power supply subsystem and a stress and culture subsystem, and communication between the underwater vehicle and the large-scale organism stress and culture device is realized by using a lora underwater antenna; logic control of stress and culture is realized by using the control and power supply subsystem; a multi-channel valve and a peristaltic pump are used for realizing fluid and culture of a solution; the hot stab male head and the hot stab female head are used for achieving communication of solution channels, and the solution replacement requirement is met. The deep-sea large-scale organism in-situ experimental device integrates wireless communication, organism acquisition, organism culture and stress, and meets the experimental requirements of environment stress and long-time sequence culture of large-scale organisms in a deep-sea ecosystem; and the large biological stress and culture device can realize underwater wireless communication, process change can be carried out according to requirements, meanwhile, solution replacement can be carried out, and a more flexible operation scheme can be realized.

Description

Technical Field

[0001] This invention belongs to the technical field of in-situ experimental research on large-scale biological stress and culture in the deep sea, specifically a large-scale biological stress and culture device based on wireless communication from an underwater vehicle. Background Technology

[0002] The deep sea lacks sunlight and has high hydrostatic pressure, creating a dark, cold, and high-pressure environment. Because photosynthesis is impossible, deep-sea ecosystems lack photosynthetic plants and herbivores, consisting only of detritivores and carnivores, heterotrophic microorganisms, and a small number of filter feeders. Deep-sea ecosystems discovered by humans include: deep-sea seamount ecosystems, deep-sea chemosynthetic ecosystems, abyssal ecosystems, submarine volcanic ecosystems, and submarine lake ecosystems.

[0003] The deep sea harbors immense marine biodiversity. 90% of the species found in deep-sea samples are novel, making the deep sea a vast treasure trove of marine biodiversity and a crucial site for research into new theories of biological evolution. The deep sea is an important area for enhancing scientific understanding, a new avenue for the discovery and utilization of strategic resources, and a significant window showcasing national marine science and technology strength. However, there are relatively few domestic projects on stress and culture devices for deep-sea organisms. For example, the paper "A Large-Scale In-situ Deep-Sea Organism Culture Device Based on ROV and Its Usage Method," published on April 9, 2021 (publication number CN112616757A), involves manual solution injection and external communication. The paper "A Long-Term Deep-Sea In-situ Large-Scale Organism Stress Culture Device and Its Usage Method," published on January 15, 2021 (publication number CN112219780A), involves automatic solution injection and external communication, but lacks underwater communication, requires setting the process on shore, and cannot change the process. Furthermore, the solution cannot be refilled or replaced after use. Utility Model Content

[0004] In response to the current technical bottlenecks encountered in deep-sea life science research, the purpose of this invention is to provide a large-scale biological stress and cultivation device based on wireless communication from an underwater vehicle.

[0005] The objective of this utility model is achieved through the following technical solution:

[0006] This invention includes a wireless communication subsystem, a control and power supply subsystem, and a stress and cultivation subsystem. The wireless communication subsystem includes a LoRa underwater antenna A, a LoRa control system and power module at the submersible end, and the submersible system itself. The LoRa control system and power module at the submersible end are connected to the LoRa underwater antenna A and the submersible system respectively via cables. The control and power supply subsystem includes a LoRa underwater antenna B, a control system and power module, an electromagnetic switch, a multi-channel valve, and a peristaltic pump. The control system and power module are connected to the LoRa underwater antenna B, the electromagnetic switch, the multi-channel valve, and the peristaltic pump respectively via cables. The pumps are connected, and the multi-channel valve has one multi-channel valve universal port and multiple multi-channel valve outlets. The peristaltic pump inlet is connected to the multi-channel valve universal port, and the peristaltic pump outlet is connected to a hotstab male connector through a solution output pipeline. One outlet of each multi-channel valve is connected to the outside, and multiple outlets are connected to a pre-stored solution through solution pipelines. The stress and culture subsystem includes multiple individual stress and culture chambers, and each individual stress and culture chamber includes a stress culture tank, which is connected to a hotstab female connector through a stress culture tank pipeline.

[0007] The control and power subsystem further includes a preparatory solution, which is connected to another hotstab male connector via a preparatory solution pipeline; another outlet of the multi-channel valve is connected to another hotstab female connector, which is paired with another hotstab male connector.

[0008] The Hotstab male connector includes a T-shaped handle, an upper wire rope fixing block, a wire rope, a lower wire rope fixing block, a fixing lug, a male connector body, and a nylon post. The upper wire rope fixing block is connected to the lower end of the T-shaped handle. The lower wire rope fixing block is detachably installed on the upper end of the male connector body. The lower end of the male connector body is threaded with a nylon post. The upper and lower ends of the wire rope are respectively connected to the upper and lower wire rope fixing blocks. The male connector body has a fixing lug for locking with the Hotstab female connector. A channel opening is provided on the male connector body below the fixing lug. O-rings A and B are respectively fitted on the upper and lower sides of the channel opening. An input port is installed on the lower wire rope fixing block, which passes through the male connector body and communicates with the channel opening.

[0009] The upper end fixing block of the wire rope has an internal hollow structure. A wire rope fixing hole communicating with the interior is opened radially on the upper end fixing block. A wire rope fixing bolt for fixing the upper end of the wire rope is provided in the wire rope fixing hole. The lower end of the wire rope is inserted into the lower end fixing block and fixed by the lower end fixing bolt.

[0010] The male steel body has symmetrically fixed lugs on both sides of its axial cross section, and the axial center line of the fixed lugs intersects perpendicularly with the axial center line of the male steel body.

[0011] The portion of the male steel body that is inserted into the hotstab female head is also fitted with an O-ring for a secure connection with the hotstab female head.

[0012] The Hotstab female connector includes a female connector steel body, which has an internal hollow structure and an axial through hole. A flow channel is formed radially on the female connector steel body, one end of which is connected to the through hole, and the other end of which is connected to an external pipeline interface. A fixing groove is formed on the top of the female connector steel body for locking and connecting with the Hotstab male connector.

[0013] The fixing groove is L-shaped, and the top of the vertical side of the L-shape is flared.

[0014] The advantages and positive effects of this utility model are as follows:

[0015] This invention is a large-scale in-situ experimental device for deep-sea organisms that integrates wireless communication, biological acquisition, biological culture, and stress testing. It meets the needs of environmental stress and long-term culture experiments for large organisms in deep-sea ecosystems. Furthermore, the large-scale biological stress and culture device can achieve underwater wireless communication, and the process can be modified according to requirements. It can also replace solutions, enabling a more flexible operating scheme. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the wireless communication subsystem of this utility model;

[0017] Figure 2 This is a schematic diagram of the control and power supply subsystem of this utility model;

[0018] Figure 3 This is a schematic diagram of the stress and culture subsystem of this utility model;

[0019] Figure 4 This is a three-dimensional structural diagram of the Hotstab male and Hotstab female connectors of this utility model in the unconnected state.

[0020] Figure 5 This is a front view of the structure of the Hotstab male and Hotstab female connectors of this utility model in the unconnected state.

[0021] Figure 6 for Figure 5 The right view;

[0022] Figure 7This is a three-dimensional structural diagram of the Hotstab male and Hotstab female connectors of this utility model in the plugging state.

[0023] Figure 8 This is a front view of the structure of the Hotstab male and Hotstab female connectors in the plug-in state of this utility model;

[0024] Figure 9 for Figure 8 The right view;

[0025] Figure 10 This is a cross-sectional view of the internal structure of the hotstab female connector of this utility model;

[0026] Wherein: 51 is the wireless communication subsystem, 1 is the LoRa underwater antenna A, 2 is the LoRa control system and power module at the submersible end, and 3 is the submersible system.

[0027] 52 is the control and power subsystem, 4 is the Lora underwater antenna B, 5 is the control system and power module, 6 is the electromagnetic switch, 9 is the external pipeline, 10 is the multi-channel valve, 11 is the multi-channel valve port, 12 is the multi-channel valve universal port, 13 is the pre-stored solution, 14 is the solution pipeline, 15 is the female Hotstab connector, 16 is the male Hotstab connector, 17 is the preparatory solution pipeline, 18 is the preparatory solution, 19 is the peristaltic pump inlet, 20 is the peristaltic pump, 21 is the peristaltic pump outlet, and 22 is the solution output pipeline.

[0028] 53 is the stress and culture subsystem, 23 is the stress culture tank piping, 24 is the stress culture tank, and 25 is the individual stress and culture chamber.

[0029] 26 is a T-shaped handle, 27 is a wire rope upper end fixing block, 28 is an input port, 29 is a wire rope upper end fixing bolt, 30 is a wire rope, 31 is a fixing bolt, 32 is a wire rope lower end fixing bolt, 33 is a wire rope lower end fixing block, 34 is a fixing ear, 35 is a male steel body, 36 is an O-ring A, 37 is an O-ring B, 38 is an O-ring C, 39 is an O-ring D, 40 is a nylon column, 41 is a channel opening, 42 is a fixing groove, 43 is a female steel body, 44 is an external pipeline interface, 45 is a female guide surface, 46 is a flow channel, 47 is a sealing surface A, 48 is a solution flow chamber, and 49 is a sealing surface B. Detailed Implementation

[0030] The present invention will now be described in further detail with reference to the accompanying drawings.

[0031] like Figures 1-3As shown, this utility model includes a wireless communication subsystem 51, a control and power supply subsystem 52, and a stress and culture subsystem 53. The wireless communication subsystem 51 includes a LoRa underwater antenna A1, a LoRa control system and power module 2 at the submersible end, and a submersible system 3. The LoRa underwater antenna A1 is connected to the LoRa control system and power module 2 at the submersible end via a coaxial cable. The LoRa control system and power module 2 at the submersible end are connected to the submersible system 3 via an 8-core cable. The control and power supply subsystem 52 is used for solution storage and solution flow setting. The control and power supply subsystem 52 includes a LoRa underwater antenna B4, a control system and power module 5, an electromagnetic switch 6, a multi-channel valve 10, and a peristaltic pump 20. The LoRa underwater antenna B4 is connected to the control system and power module 5 via a coaxial cable. The control system and power module 53 are connected to the LoRa control system and power module 53. The source module 5 is connected to the electromagnetic switch 6, the multi-channel valve 10, and the peristaltic pump 20 via multi-core cables. The multi-channel valve 10 has one multi-channel valve universal port 12 and multiple multi-channel valve outlets 11. The peristaltic pump inlet 19 of the peristaltic pump 20 is connected to the multi-channel valve universal port 12. The peristaltic pump outlet 21 of the peristaltic pump 20 is connected to a hotstab male connector 16 via a solution output pipeline 22. One of the outlets 11 of each multi-channel valve is connected to the outside, and multiple outlets are connected to the pre-stored solution 13 via solution pipelines 14. The stress and culture subsystem 53 includes multiple individual stress and culture chambers 25 for the stress and culture of organisms. Each individual stress and culture chamber 25 includes a stress culture tank 24, which is connected to a hotstab female connector 15 via a stress culture tank pipeline 23.

[0032] In this embodiment, the multi-channel valve 10 is an eight-channel valve with eight outlets. The first outlet is connected to the outside via an external pipe 9. The second to fifth outlets are each connected to a pre-stored solution 13 via a solution pipe 14. The sixth outlet is connected to another hotstab connector 15. The seventh and eighth outlets are either standby or each connected to a pre-stored solution 13 via a solution pipe 14. The eight-channel valve and peristaltic pump 20 complete the flow and switching of the pre-stored solution, allowing for the replacement and injection of the pre-stored solution 13 according to settings.

[0033] The control and power subsystem 52 of this embodiment also includes a reserve solution 18. The reserve solution 18 is connected to another hotstab male connector 16 via a reserve solution pipeline 17. The other hotstab male connector 16 is paired with another hotstab female connector 15 connected to the outlet of the sixth channel valve of the eight-channel valve. The reserve solution 18 is a backup solution. When the pre-stored solution 13 is insufficient to complete the solution filling according to the predetermined plan, the reserve solution 18 is activated to replenish the solution or set a new plan. The reserve solution 18 is carried into the water later by the submersible for replenishment and reserve for new plans. In this embodiment, the pre-stored solution 13 and the reserve solution 18 can be mercury solutions.

[0034] In this embodiment, the LoRa underwater antenna A1 and LoRa underwater antenna B2 are used in pairs. LoRa underwater antenna A1 is connected to the submersible system 3, and its communication and control are handled by the submersible system 3. LoRa underwater antenna B2 is connected to the control system and power module 5, and its communication and control are handled by the control system and power module 5. Using LoRa underwater antennas A1 and B2 enables communication between the submersible system 3 and the control system and power module 5. The submersible system 3, control system, and power module 5 in this embodiment are all existing technologies and will not be described in detail here.

[0035] The multi-channel valve 10 in this embodiment is prior art and can be the "multi-channel rotary valve" disclosed in CN216778182U, published on June 21, 2022. The electromagnetic switch 6 in this embodiment is prior art and can be the "press-type electromagnetic switch device" disclosed in CN112311377A, published on February 2, 2021. The stress culture tank 24 in this embodiment is prior art and can be the "large-scale in-situ deep-sea biological stress device based on ROV" disclosed in CN112189615A, published on January 8, 2021.

[0036] like Figures 4-10As shown, the Hotstab male connector 16 in this embodiment includes a T-shaped handle 26, an upper wire rope fixing block 27, a wire rope 30, a lower wire rope fixing block 33, a fixing lug 34, a male connector steel body 35, and a nylon post 40. The topmost part is the T-shaped handle 26. The upper wire rope fixing block 27 is welded and fixed to the lower end of the T-shaped handle 26. The upper wire rope fixing block 27 has an internally hollow structure, and radially arranged wire rope fixing holes communicating with the interior are provided on the upper wire rope fixing block 27. The upper end of the wire rope 30 is inserted into the upper wire rope fixing block 27. A lower wire rope fixing bolt 32 for fixing the upper end of the wire rope 30 is provided in the wire rope fixing hole. The lower wire rope fixing block 33 and the male connector steel body 35 are separate structures. Four fixing bolts 31 are installed on the lower wire rope fixing block 33, and the four fixing bolts 31 are detachably connected to the upper end of the male connector steel body 35. The lower end of the wire rope 30 is inserted into the lower wire rope fixing block 33 and fixed by the lower wire rope fixing bolts 32. A nylon post 40 is threaded onto the lower end of the male connector steel body 35. Fixing ears 34 are symmetrically fixed to both sides of the axial section of the male connector steel body 35. In this embodiment, the fixing ears 34 are cylindrical and are used to connect and lock with the hotstab female connector 15. The axial center line of the fixing ears 34 intersects perpendicularly with the axial center line of the male connector steel body 35. A channel opening 41 is provided on the male steel body 35 below the fixing lug 34. O-rings A36 and B37 are respectively fitted on the male steel body 35 on the upper and lower sides of the channel opening 41. An input port 28 is installed on the fixing block 33 at the lower end of the wire rope. One end of the input port 28 is connected to the solution output pipeline 22 or the preparatory solution pipeline 17, and the other end of the input port 28 passes through the male steel body 35 and communicates with the channel opening 41. The part of the male steel body 35 inserted into the hotstab female head 15 is also fitted with O-rings C38 and D39 for a secure connection with the hotstab female head 15. In this embodiment, the wire rope 30 is a short 316 stainless steel wire rope welded at both ends, which plays a buffering role when the robot arm operates the T-shaped handle 26 to perform a twisting action.

[0037] The Hotstab female connector 15 in this embodiment includes a female connector steel body 43, which has an internal hollow structure and an axial through hole. A flow channel 46 is radially formed on the female connector steel body 43. One end of the flow channel 46 is connected to the through hole, and the other end of the flow channel 46 is connected to an external pipeline interface 44. The external pipeline interface 44 is used to connect to the stress culture tank pipeline 23 or the multi-channel valve 10. A fixing groove 42 is formed on the top of the female connector steel body 43 for connecting and locking with the Hotstab male connector 16. In this embodiment, there are two fixing grooves 42, which are symmetrically arranged on opposite sides of the Hotstab female connector 15. Each fixing groove 42 corresponds to a fixing lug 34. Each fixing groove 42 is "L" shaped, and the top of the vertical side of the "L" shape is flared. When the hotstab male connector 16 and the hotstab female connector 15 are connected, the retaining lug 34 is inserted from the flared end to the bottom of the vertical side of the "L" shape. Using the T-shaped handle 26, the hotstab male connector 16 is twisted, causing the retaining lug 34 to slide within the retaining groove 42 to the end of the horizontal side of the "L" shape, thus locking the hotstab male connector 16 and the hotstab female connector 15. To unlock, first twist the hotstab male connector 16 in the opposite direction, causing the retaining lug 34 to rotate to the bottom of the vertical side of the "L" shape, then pull out the hotstab male connector 16 to complete the unlocking. In this embodiment, the through-hole inside the hotstab female connector 15 has, from top to bottom, a female connector guide surface 45, a sealing surface A47, a solution flow chamber 48, and a sealing surface B49. One end of the flow channel 46 connects to the solution flow chamber 48. The female connector guide surface 45 is an inwardly sloping surface from top to bottom. The hardness of the nylon column 40 is much less than that of the female steel body 43. When the hotstab male connector 16 and the hotstab female connector 15 are inserted, the design of the female connector guide surface 45 plays a guiding role, which facilitates the insertion of the hotstab male connector 16. When the hotstab male connector 16 and the hotstab female connector 15 are locked, the channel opening 41 is located in the solution flow chamber 48. O-rings A36 and B37 are respectively sealed and abutted against the sealing surface A47 and are located on the upper and lower sides of the solution flow chamber 48. O-rings C38 and D39 are respectively sealed and abutted against the sealing surface B49 and are located below the O-ring B37.

[0038] The method of using this utility model includes the following steps:

[0039] Step A: The shore-based end is cleaned for the pipelines and each individual stress and culture chamber 25;

[0040] Step B: After cleaning, in the initial state, each stress culture tank 24 is filled with seawater and the pre-stored solution 13 is installed; the wireless communication subsystem 51 is installed on the submersible, and the LoRa underwater antenna A1 is attached to the submersible's robotic arm.

[0041] Step C: Use the submersible to carry the control and power subsystem 52 and the stress and culture subsystem 53 to the seabed respectively. Use the robotic arm on the submersible to insert the hotstab male connector 16 in the control and power subsystem 52, which is connected to the solution output pipeline 22, into the hotstab female connector 15 in the single stress and culture chamber 25.

[0042] Step D: Use the robotic arm on the submersible from Step C to open the stress culture tank 24 in the single stress and culture chamber 25, grab a large organism and put it into the stress culture tank 24, and then close the stress culture tank 24.

[0043] Step E: The control system and power module 5 perform large-scale biological stress culture according to the pre-set operating procedure. A peristaltic pump 20 injects a pre-stored solution 13 into the stress culture tank 24 via solution pipeline 14, multi-channel valve universal port 12, peristaltic pump 20, solution output pipeline 22, hotstab male connector 16, hotstab female connector 15, and stress culture tank pipeline 23. During the culture process, when one pre-stored solution 13 is depleted, the multi-channel valve 10 is rotated to switch to other pre-stored solutions 13. After a... After the set time, the manipulator on the submersible in step C is used to pull out the male hotstab connector 16 connected to the solution output line 22 in the control and power subsystem 52, so that the monomer stress and culture chamber 25 injected with the pre-stored solution are independent and brought back to the deck by the submersible; at the same time, the manipulator on the submersible in step C is used to grab the male hotstab connector 16 connected to the solution output line 22 in the control and power subsystem 52 and insert it into the female hotstab connector 15 connected to another monomer stress and culture chamber 25, and the experiment continues;

[0044] Step F: Change to another predetermined process; one way to change is to use the robotic arm on the submersible in step C to press the button on the electromagnetic switch 6, and the control system and power module 5 change the corresponding predetermined process; the second way to change is to use the Lora underwater antenna A1 attached to the robotic arm of the submersible in step B and the Lora underwater antenna B4 on the submersible in step C to communicate, reset the process, and proceed to the next process setting.

[0045] Step G: After the entire operation is completed, use the robotic arm on the submersible from Step C to close the stress culture tank 24 into which the large organism was placed, detach all hotstab female heads 15 and hotstab male heads 16, so that the wireless communication subsystem 51, control and power subsystem 52, and stress and culture subsystem 53 become independent systems, and bring them back to the deck in batches for sampling.

[0046] In step E, if the pre-stored solution 13 in the pipeline has been completely consumed, or if a new solution is needed, the submersible brings the pre-prepared solution 18 to the seabed. The robotic arm on the submersible grabs the pre-prepared solution 18 and inserts it into the hotstab male connector 16 connected to the pre-prepared solution pipeline 17 and the hotstab female connector 15 connected to the multi-channel valve 10 to continue the solution replacement. Then, follow step F to reset the process and proceed to the next process setting.

[0047] The submersibles used in the above steps can be different submersibles, which can enter the sea at the same time or one after the other.

[0048] This invention addresses the current technological bottlenecks in deep-sea life science research by overcoming key technological challenges, including short-range wireless communication and solutions flow and underwater solution replacement in in-situ closed systems. Ultimately, it enables the stress and culture of organisms, resolving the issues of insufficient in-situ large-scale biological experiments and time efficiency in my country, which hinders the in-situ stress culture of large organisms. The stress culture device of this invention is designed for underwater vehicle operation, using ROV or HOV for easy portability, and is suitable for complex seabed environments at depths of up to 6000m, varying with temperature and ocean currents. It meets the needs of environmental stress and long-term culture experiments for large organisms in deep-sea ecosystems. Furthermore, the large-scale biological stress and culture device enables underwater wireless communication, allowing for process modifications and solution replacement as needed, resulting in a more flexible operational approach.

Claims

1. A large-scale biological stress and culture device based on underwater vehicle wireless communication, characterized in that: The system includes a wireless communication subsystem (51), a control and power supply subsystem (52), and a stress and cultivation subsystem (53). The wireless communication subsystem (51) includes a Lora underwater antenna A (1), a Lora control system and power module (2) at the submersible end, and a submersible system (3). The Lora control system and power module (2) at the submersible end are connected to the Lora underwater antenna A (1) and the submersible system (3) respectively via cables. The control and power supply subsystem (52) includes a Lora underwater antenna B (4), a control system and power module (5), an electromagnetic switch (6), a multi-channel valve (10), and a peristaltic pump (20). The control system and power module (5) are connected to the Lora underwater antenna B (4), the electromagnetic switch (6), the multi-channel valve (10), and the peristaltic pump (20) respectively via cables. The channel valve (10) has one multi-channel valve universal port (12) and multiple multi-channel valve outlets (11). The peristaltic pump inlet (19) of the peristaltic pump (20) is connected to the multi-channel valve universal port (12). The peristaltic pump outlet (21) of the peristaltic pump (20) is connected to a hotstab male connector (16) through a solution output pipeline (22). One of the outlets (11) of each multi-channel valve is connected to the outside, and multiple outlets are connected to the pre-stored solution (13) through solution pipelines (14). The stress and culture subsystem (53) includes multiple individual stress and culture chambers (25). Each individual stress and culture chamber (25) includes a stress culture tank (24). The stress culture tank (24) is connected to a hotstab female connector (15) through a stress culture tank pipeline (23).

2. The large-scale biological stress and culture device based on underwater vehicle wireless communication according to claim 1, characterized in that: The control and power subsystem (52) also includes a preparatory solution (18) which is connected to another hotstab male connector (16) via a preparatory solution line (17); another channel of the multi-channel valve outlet (11) is connected to another hotstab female connector (15), which is paired with another hotstab male connector (16).

3. The large-scale biological stress and culture device based on underwater vehicle wireless communication according to claim 1, characterized in that: The Hotstab male connector (16) includes a T-shaped handle (26), an upper wire rope fixing block (27), a wire rope (30), a lower wire rope fixing block (33), a fixing lug (34), a male connector body (35), and a nylon post (40). The upper wire rope fixing block (27) is connected to the lower end of the T-shaped handle (26). The lower wire rope fixing block (33) is detachably installed on the upper end of the male connector body (35). The lower end of the male connector body (35) is threadedly connected to the nylon post (40). The upper and lower ends of the wire rope (30) are respectively fixed to the upper end of the wire rope. The block (27) and the lower end fixing block (33) of the wire rope are connected; the male steel body (35) is provided with a fixing ear (34) for connecting and locking with the hotstab female head (15), and a channel opening (41) is opened on the male steel body (35) below the fixing ear (34). O-rings A (36) and O-rings B (37) are respectively fitted on the male steel body (35) on the upper and lower sides of the channel opening (41); an input port (28) is installed on the lower end fixing block (33) of the wire rope, and the input port (28) passes through the male steel body (35) and communicates with the channel opening (41).

4. The large-scale biological stress and culture device based on underwater vehicle wireless communication according to claim 3, characterized in that: The upper end fixing block (27) of the wire rope has a hollow internal structure. The upper end fixing block (27) of the wire rope has a wire rope fixing hole that communicates with the interior along the radial direction. The upper end fixing hole of the wire rope is provided with a lower end fixing bolt (32) for fixing the upper end of the wire rope (30). The lower end of the wire rope (30) is inserted into the lower end fixing block (33) of the wire rope and fixed by the lower end fixing bolt (32).

5. The large-scale biological stress and culture device based on underwater vehicle wireless communication according to claim 3, characterized in that: The male steel body (35) has symmetrical fixed ears (34) on both sides of its axial cross section, and the axial center line of the fixed ears (34) intersects perpendicularly with the axial center line of the male steel body (35).

6. The large-scale biological stress and culture device based on underwater vehicle wireless communication according to claim 3, characterized in that: The portion of the male steel body (35) inserted into the hotstab female head (15) is also fitted with an O-ring for secure connection with the hotstab female head (15).

7. The large-scale biological stress and culture device based on underwater vehicle wireless communication according to claim 1, characterized in that: The Hotstab female connector (15) includes a female connector steel body (43), which has an internal hollow structure and a through hole along the axial direction. A flow channel (46) is provided on the female connector steel body (43) along the radial direction. One end of the flow channel (46) is connected to the through hole, and the other end of the flow channel (46) is connected to an external pipeline interface (44). A fixing groove (42) for connecting and locking with the Hotstab male connector (16) is provided on the top of the female connector steel body (43).

8. The large-scale biological stress and culture device based on underwater vehicle wireless communication according to claim 7, characterized in that: The fixing groove (42) is "L" shaped, and the top of the vertical side of the "L" shape is flared.

Images